Skip to content

Commit

Permalink
Update README.md
Browse files Browse the repository at this point in the history
  • Loading branch information
terrimporter authored Oct 30, 2019
1 parent 2cbe887 commit 1ee74e1
Showing 1 changed file with 1 addition and 1 deletion.
2 changes: 1 addition & 1 deletion README.md
Original file line number Diff line number Diff line change
Expand Up @@ -28,7 +28,7 @@ If you are comfortable reading code, read through the snakefile to see how the p

Raw paired-end reads are merged using SEQPREP v1.3.2 from bioconda (St. John, 2016). This step looks for a minimum Phred quality score of 20 in the overlap region, requires at least 25bp overlap.

Primers are trimmed in two steps using CUTADAPT v2.4 from bioconda (Martin, 2011). This step looks for a minimum Phred quality score of at least 20 at the ends, forward primer is trimmed first, no more than 3 N's allowed, trimmed reads need to be at least 150 bp, untrimmed reads are discarded. The output from the first step, is used as in put for the second step. This step looks for a minimum Phred quality score of at least 20 at the ends, the reverse primer is trimmed, no more than 3 N's allowed, trimmed reads need to be at least 150 bp, untrimmed reads are discarded.
Primers are trimmed in two steps using CUTADAPT v2.6 from bioconda (Martin, 2011). This step looks for a minimum Phred quality score of at least 20 at the ends, forward primer is trimmed first, no more than 3 N's allowed, trimmed reads need to be at least 150 bp, untrimmed reads are discarded. The output from the first step, is used as in put for the second step. This step looks for a minimum Phred quality score of at least 20 at the ends, the reverse primer is trimmed, no more than 3 N's allowed, trimmed reads need to be at least 150 bp, untrimmed reads are discarded.

Files are reformatted and samples are combined for a global analysis.

Expand Down

0 comments on commit 1ee74e1

Please sign in to comment.