Get Data
First step is to import the data to be analysed in the Galaxy Server.
If you don't have an account, please, register and log in. You'll be able to access all platform services, including the list of published workflows, restricted to logged users.
- Click on GET DATA, at the left side and select "Upload File". That will open the next window:
- Choose or drop your local files and define for each file its "Type" (i.e. BAM, RData, GFF) and "Genome" (i.e. Saccharomyces Cerevisiae (R64-1-1 GCA_000146045.2)). MNase-seq aligned reads in a BAM file is the primary data of the Nucleosome Dynamics toolkit.
ℹ️ Learn more about the input files of each Nucleosome Dynamics tool at the landing page
We supply an example dataset for testing Nucleosome Dynamics tools. It corresponds to MNase-seq data for S. cerevisiae cells synchronized in G1 and S phase, as described by Deniz (2016).
- Raw data available under accession number SAMEA2698380
- Deniz, Ö., Flores, O., Aldea, M., Soler-López, M., and Orozco, M. (2016). Nucleosome architecture throughout the cell cycle. Scientific Reports 6, 19729.
- Click on Shared Data -> Data Libraries at the top menu of the page
- Choose "Nucleosome Dynamics: test data"
- Select all the files and add them into your history as is shown at the following image
