Poreplex 0.3

New features in 0.3

• Reads now can be demultiplexed without basecalling.
• With --polya turned on, poly(A) tail dwell time is written to sequencing_summary.txt. More detailed information is written to the dumped events by the --dump-basecalled-events switch.
• Shows detailed error messages in poreplex.log.
• The files generated by --dump-basecalled-events now includes the attributes for signal scaling parameters and the last position that DNA adapter ends within the signal.
• Extremely short sequences are now sorted into failed reads. The minimum required length can be changed with the --minimum-length switch. (Suggested by Nathan Roach)

Changes in 0.3

• Fixed a segmentation fault when using albacore 2.3.3.
• Fixed an error that stops overall process by an invalid FAST5 file.
• filename in sequencing_summary.txt is now shown as a relative path from the output directory, not from the subdirectory for a read group.
• Fixed a problem that separate lines of FASTA, FASTQ or sequencing-summary.txt are mixed up in the output file sometimes.
• Turned off the chimeric read filter by default. Now the --filter-chimera option turns it back on.
• Updated the neural network model for barcode demultiplexing for even less false positives using randomly stitched signal fragments as a background.