Dissociable contributions of the amygdala and ventral hippocampus to stress-induced changes in defensive behavior

Pennington ZT, LaBanca AR, Sompolpong P, Christenson Wick Z, Feng Y, Francisco TR, Chen L, Fulton SL, Maze I, Dong Z, Cai DJ (2023)

Nash Family Department of Neuroscience, Icahn School of Medicine at Mount Sinai

Data and Analysis

All data and analysis are available here for reproduction and further analysis.

Analysis files

All analysis were conducted using RStudio, using the following packages: ggplot2, ez, tidyr, dplyr, purrr, lmtest, car, knitr.

Each experimental folder has a single analysis file (.rmd). When the working directory is set to .rmd location, file can be run to load in all experimental data and reproduce analysis/plots.

A brief description of each experiment is provided below.

For each figure, we highlight the respective experiments included.

Figures

List of experiments in each figure

Figure 1

• 1c1d (panels B-E)
• 10j10e3 (panels F-H)

Figure 2

• 10g2g3 (panels B-E)
• 10j10e3 (panels F-J)

Figure 3

• 10k6 (panels A, F-J)
• 10k9 (panels B)
• 10k6c (panels C-E)

Figure 4

• 10n5b (all panels)

Figure S1

• 1c1d (all panels)

Figure S2

• 1f (all panels)

Figure S3

• 10j10e3 (all panels)

Figure S4

• 10j10e3 (all panels)

Figure S5

• 10e (all panels)

Figure S6

• 10e (all panels)

Figure S7

• 10k6 (all panels)

Figure S8

• 10k5 (panels B-C)
• 10k (panels E-H)

Figure S9

• 10k6b (panels B-D)
• 10k2 (panels E-F)

Experiments

A very brief description of experiments. See manuscript for details.

1c1d

Basic behavioral effects showing influence of initial stressor/trauma is amplitude dependent and not influenced by sex

1f

Learning rate analysis

10e

Exps demonstrating anisomycin in amygdala blocks learning without altering expression, and also blocks IEG protein expression.

10g2g3

Anisomycin given systemically after trauma. A week later, animals tested in light-dark test, trauma memory recall, and novel stressor and subsequent novel stressor recall. Note that although this experiment includes animals given anisomycin either 2 or 3 times after trauma, only animals receiving 3 injections of anisomycin are included in manuscript.

10j10e3

Anisomycin in either the BLA (10e3) or vHC (10j) just after trauma. A week later, animals tested in light-dark test, trauma memory recall, and novel stressor test and subsequent novel stressor recall. Additionally, controls from these experiemnts given vehicle were utilized to show normative effects and relationships between phenotypes. For main analysis, controls with vehicle in vHC or BLA were collapsed after being shown to not be different.

10k

Effect of inhibiting either BLA or vHC on light-dark test and novel stressor using PSAM.

10k2

Effect of inhibiting either BLA or vHC on light-dark, open field, and elevated plus maze tests using PSAM.

10k5

Effect of inhibiting either BLA or vHC during trauma memory recall using PSAM. Note animals also received uPSEM or vehicle with novel stressor at end of experiment (currently low N).

10k6

Effect of inhibiting either BLA or vHC on light-dark test and novel stressor using HM4D.

10k6b

Effect of inhibiting either BLA or vHC on light-dark, open field, and elevated plus maze tests using HM4D.

10k6c

Effect of inhibiting either BLA or vHC on fear memory recall.

10k9

Validation of HM4Di-mediated inhbition of BLA/vHC with in slice.

10n5b

Inhibition of BLA->vHC and vHC->BLA projections on light-dark test and novel stressor using HM4D.