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CharlesJB/ChIP-Seq-Analysis
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This pipeline will use previously aligned data and will perform those steps: 1- Peak calling (default: macs) 2- Annotation (R's ChIPpeakAnno) 3- Selection of the regions in the proximal promoter To use the pipeline: 1- Go to the directory where you wish to store the results. 2- Create a file named config.txt. In this file, write the name of the files to analyze. One (treatment only) or two (treatment and control) file per line. 3- Call the init script situated in this repository: i.e.: ~/git-clones/ChIP-Seq-Analysis/init 4- Type "make" If you have many processors avaible, you can use the "-j" option when calling make: make -j4 Dependencies: * Rscript (http://stat.ethz.ch/R-manual/R-patched/library/utils/html/Rscript.html) * MACS (http://liulab.dfci.harvard.edu/MACS/index.html) * ChIPpeakAnno (http://bioconductor.org/packages/release/bioc/html/ChIPpeakAnno.html) Note: All the programs must be installed before starting the pipeline. Your PATH environment variable should contain the path to every dependency (except for R packages).
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