BRILIA v3.5.9

B-cell Repertoire Inductive Lineage and Immunosequence Annotator

BRILIA is designed to be a single-platform software capable of processing B-cell receptor sequences, annotating VDJ junctions, assigning B-cell lineages/clonotypes, and characerizing B-cell repertoires.

REFERENCE
Lee, D.W., I. Khavrutskii, A. Wallqvist, S. Bavari, C. Cooper, and S. Chaudhury. BRILIA: Integrated Tool for High-Throughput Annotation and Lineage Tree Assembly of B-Cell Repertoires. Frontiers in Immunology, 2017. 7(681).

CONTACT
E-mail: brilia@bhsai.org (address to Donald Lee for BRILIA help, or Sid Chuadhury for all other questions)
Hours: M-F, 9am-5pm EST, off on federal holidays

Inputs

• Accepts DNA/RNA sequence files with the following extensions:
  • .fasta or .fa
  • .fastq (see MinQuality input parameter to treat low-quality base reads as "N" bases)
  • .csv (comma-delimited)
  • .ssv (semicolon-delimited)
  • .tsv (tab-delimited)
• Does not accept unassembled, paired-end reads. These should be assembled using another software.
• Non-ACGTU letters are treated as wildcard "N" bases. Special letters are not used yet (Ex: R for A/G will be treated as N)
• For delimited files, make sure that the first row is the data header. Ex: "SeqName, H-Seq, L-Seq, TemplateCount"
  • SeqName: the name of the sequence
  • H-Seq: heavy chain sequence
  • L-Seq: light chain sequence
  • TemplateCount: the number of sequence copies (Optional)
• See example input files

Outputs

• Returns 3 delimited csv file:
  • [output_file_name].BRILIAv3.csv : final annotation and phylogeny data of productive V(D)J sequences
  • [output_file_name].BRILIAv3.Raw.csv : initial annotation of V(D)J sequences without lineage-base annotation correction.
  • [output_file_name].BRILIAv3.Err.csv : non-productive VDJ sequences and any sequences that could not be annotated fully.
• If the output file is not specified, files will be stored in a subfolder with the same name as the input file.
• See output file header info
• See example output files

Installing BRILIA

1. Dowload & install the MATLAB Runtime R2017a(9.2) from MathWorks into a folder, referred to as RUNTIME_FOLDER.
2. Download & unzip the BRILIA exe files into a folder, referred to as BRILIA_FOLDER.
3. Open the OS terminal and navigate to BRILIA_FOLDER.
4. Run BRILIA. If the OS asks for permission to modify files, "cancel" should work. See here for more details.
   Windows> BRILIA.exe
Linux$ run_BRILIA.sh RUNTIME_FOLDER
   You should end up with a prompt like this:
   BRILIA>

Using BRILIA

Assuming the files are structured as such:  
/home/user/my files/file1.fastq
                    file2.fastq
                    fileN.fastq
                    
Output files will be placed as such:
/home/user/my files/file1/file1.BRILIAvN.csv
                    file2/file2.BRILIAvN.csv
                    fileN/fileN.BRILIAvN.csv


BRILIA> ?    (see help)
BRILIA> cd "/home/user/my files"    (go to sequece file parent folder. Use quotes "" if there is a space in the path)
BRILIA> "./file*.fastq" -chain h -species mouse -strain balb    (process heavy chain for mouse balb/c)

 Developed at BHSAI (bhsai.org)
 Website: https://github.com/bhsai/brilia
 Status: ...
 Status: Finished in 3.3 sec.

BRILIA> mergeSimilarSeq "./*" 0.01  (optional: parent-child sequences with < 1% Hamming distance will be merged under the parent)

BRILIA> runAnalysis "./*"   (run analysis on all output files in subfolder of this current folder)

  Status: Drawing 46 trees.
  Status: ...

BRILIA> exit 

Tips

1. Consider adding BRILIA_FOLDER ( and RUNTIME_FOLDER for Linux ) to the OS PATH to call BRILIA easily.
2. You could skip entering the BRILIA> prompt by adding inputs after the command in step 4. Example:
   Windows> BRILIA.exe species mouse chain h ...
Linux$ run_BRILIA.sh RUNTIME_FOLDER species mouse chain h ...
3. To test if BRILIA is working, try the following:
   BRILIA> test all
   A folder called Examples will be generated in the BRILIA_FOLDER and BRILIA will process these folders.
4. For Linux, make sure executable files have read (r) + execute (x) permissions. Add permissions if needed.

   BRILIA_FOLDER$ chmod +rx BRILIA
   BRILIA_FOLDER$ chmod +rx run_BRILIA.sh
   

License, Patches, and Ongoing Developements

• The program is distributed under the GNU General Public License.
• BRILIA patch info.
• Feature requests and open issues.