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Peptide counts for multiple label-free sample comparisons #15

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potockym opened this issue Jun 15, 2024 · 1 comment
Open

Peptide counts for multiple label-free sample comparisons #15

potockym opened this issue Jun 15, 2024 · 1 comment

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@potockym
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Hello, I want to use DEqMS to analyze my LFQ dataset, which consists of five samples (each in triplicate) and analyze the DEPS among all ten possible comparison pairs. Should I use the values from the total dataset for the peptide min count, or should I always use minimum peptide counts relevant only for the compared sample pairs?
Thanks
Martin Potocky

@yafeng
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yafeng commented Jun 18, 2024

@potockym Hi, I recommend to use minimum peptide counts relevant only for the compared sample pairs.

Yafeng

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