From 1ee74e132d47df6c606dc42fee1506c6624f9489 Mon Sep 17 00:00:00 2001 From: "Teresita M. Porter" Date: Wed, 30 Oct 2019 13:12:41 -0400 Subject: [PATCH] Update README.md --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 1cf80ff..9a206ac 100644 --- a/README.md +++ b/README.md @@ -28,7 +28,7 @@ If you are comfortable reading code, read through the snakefile to see how the p Raw paired-end reads are merged using SEQPREP v1.3.2 from bioconda (St. John, 2016). This step looks for a minimum Phred quality score of 20 in the overlap region, requires at least 25bp overlap. -Primers are trimmed in two steps using CUTADAPT v2.4 from bioconda (Martin, 2011). This step looks for a minimum Phred quality score of at least 20 at the ends, forward primer is trimmed first, no more than 3 N's allowed, trimmed reads need to be at least 150 bp, untrimmed reads are discarded. The output from the first step, is used as in put for the second step. This step looks for a minimum Phred quality score of at least 20 at the ends, the reverse primer is trimmed, no more than 3 N's allowed, trimmed reads need to be at least 150 bp, untrimmed reads are discarded. +Primers are trimmed in two steps using CUTADAPT v2.6 from bioconda (Martin, 2011). This step looks for a minimum Phred quality score of at least 20 at the ends, forward primer is trimmed first, no more than 3 N's allowed, trimmed reads need to be at least 150 bp, untrimmed reads are discarded. The output from the first step, is used as in put for the second step. This step looks for a minimum Phred quality score of at least 20 at the ends, the reverse primer is trimmed, no more than 3 N's allowed, trimmed reads need to be at least 150 bp, untrimmed reads are discarded. Files are reformatted and samples are combined for a global analysis.