This code is used to calculate the differential genes of exosomes in the plasma of normal volunteers and osteosarcoma patients. The statistical method is DESeq2, and the results are presented using heat maps and volcanic maps. The raw data was saved in GEO: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE218526 The analysis result was saved in figshare: https://doi.org/10.6084/m9.figshare.22659328.v1 The BAM data was in SRA: https://identifiers.org/ncbi/insdc.sra:SRP409185