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I believe this is an intermittent issue since I was able to run this workflow before but it's crashing now. I do see a BismarkIndex directory created under the stage-xxx directory but there's not a working directory created yet for the actual bismark align step.
Here's more information from the .nextflow.log file:
Feb-06 11:47:19.101 [Actor Thread 23] DEBUG nextflow.processor.TaskProcessor - Handling unexpected condition for
task: name=NFCORE_METHYLSEQ:METHYLSEQ:FASTQ_ALIGN_DEDUP_BISMARK:BISMARK_ALIGN (1); work-dir=null
error [com.amazonaws.SdkClientException]: Unable to complete multipart download. Individual part download failed.
Feb-06 11:47:19.423 [Actor Thread 23] ERROR nextflow.processor.TaskProcessor - Error executing process > 'NFCORE_METHYLSEQ:METHYLSEQ:FASTQ_ALIGN_DEDUP_BISMARK:BISMARK_ALIGN (1)'
Caused by:
java.net.SocketTimeoutException: Read timed out
com.amazonaws.SdkClientException: Unable to complete multipart download. Individual part download failed.
at com.amazonaws.services.s3.transfer.internal.CompleteMultipartDownload.call(CompleteMultipartDownload.java:54)
at com.amazonaws.services.s3.transfer.internal.CompleteMultipartDownload.call(CompleteMultipartDownload.java:29)
...
at java.base/java.lang.Thread.run(Thread.java:1583)
Caused by: java.util.concurrent.ExecutionException: java.net.SocketTimeoutException: Read timed out
...
at com.amazonaws.services.s3.transfer.internal.CompleteMultipartDownload.call(CompleteMultipartDownload.java:47)
... 5 common frames omitted
Caused by: java.net.SocketTimeoutException: Read timed out
at java.base/sun.nio.ch.NioSocketImpl.timedRead(NioSocketImpl.java:278)
at java.base/sun.nio.ch.NioSocketImpl.implRead(NioSocketImpl.java:304)
...
... 4 common frames omitted
Feb-06 11:47:19.546 [Actor Thread 23] DEBUG nextflow.Session - Session aborted -- Cause: Unable to complete multipart download. Individual part download failed.
Feb-06 11:47:19.616 [Actor Thread 23] DEBUG nextflow.Session - The following nodes are still active:
[process] NFCORE_METHYLSEQ:METHYLSEQ:FASTQ_ALIGN_DEDUP_BISMARK:BISMARK_ALIGN
status=ACTIVE
port 0: (queue) OPEN ; channel: -
port 1: (value) bound ; channel: -
port 2: (value) bound ; channel: -
port 3: (cntrl) - ; channel: $
Description of the bug
I believe this is an intermittent issue since I was able to run this workflow before but it's crashing now. I do see a BismarkIndex directory created under the stage-xxx directory but there's not a working directory created yet for the actual bismark align step.
Here's more information from the .nextflow.log file:
Command used and terminal output
nextflow run nf-core/methylseq --input samplesheet1.csv --outdir out7 --genome GRCh38 -profile singularity -c custom.config -resume
Relevant files
my config file:
System information
N E X T F L O W ~ version 24.10.4
Launching
https://github.com/nf-core/methylseq
[dreamy_coulomb] DSL2 - revision: 4415e90 [master]The text was updated successfully, but these errors were encountered: