From 8739b9401eff7cd7408301448ef11fb7fa51e7ce Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Tue, 12 Dec 2023 10:54:00 +0100 Subject: [PATCH 001/226] Change the workflow to take MAGeCK MLE as a default and add tests --- .github/workflows/ci.yml | 2 ++ conf/modules.config | 8 +++++ conf/test_screening.config | 3 +- conf/test_screening_rra.config | 29 +++++++++++++++++ modules/local/matricescreation.nf | 53 +++++++++++++++++++++++++++++++ nextflow.config | 4 ++- nextflow_schema.json | 6 +++- workflows/crisprseq_screening.nf | 27 ++++++++++++---- 8 files changed, 122 insertions(+), 10 deletions(-) create mode 100644 conf/test_screening_rra.config create mode 100644 modules/local/matricescreation.nf diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 133e6739..9a7737fc 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -29,8 +29,10 @@ jobs: ANALYSIS: - "test_screening" - "test_screening_paired" + - "test_screening_rra" - "test_targeted" - "test_umis" + steps: - name: Check out pipeline code uses: actions/checkout@v3 diff --git a/conf/modules.config b/conf/modules.config index afae335d..f1c930e4 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -173,6 +173,14 @@ process { ] } + withName: MATRICESCREATION { + publishDir = [ + path: { "${params.outdir}/design_matrix" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + withName: MINIMAP2_ALIGN_UMI_1 { ext.args = '-x map-ont' ext.prefix = { "${reads.baseName}_cycle1" } diff --git a/conf/test_screening.config b/conf/test_screening.config index 7661aacf..0b5c5b70 100644 --- a/conf/test_screening.config +++ b/conf/test_screening.config @@ -23,7 +23,6 @@ params { input = 'https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/samplesheet_test.csv' analysis = 'screening' crisprcleanr = "Brunello_Library" - mle_design_matrix = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/design_matrix.txt" library = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/brunello_target_sequence.txt" - rra_contrasts = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/rra_contrasts.txt" + contrasts = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/rra_contrasts.txt" } diff --git a/conf/test_screening_rra.config b/conf/test_screening_rra.config new file mode 100644 index 00000000..5042ec35 --- /dev/null +++ b/conf/test_screening_rra.config @@ -0,0 +1,29 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/crisprseq -profile test_screening_rra, --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test screening profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' + + // Input data + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/samplesheet_test.csv' + analysis = 'screening' + crisprcleanr = "Brunello_Library" + library = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/brunello_target_sequence.txt" + contrasts = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/rra_contrasts.txt" + rra = true +} diff --git a/modules/local/matricescreation.nf b/modules/local/matricescreation.nf new file mode 100644 index 00000000..cb1005ef --- /dev/null +++ b/modules/local/matricescreation.nf @@ -0,0 +1,53 @@ +process MATRICESCREATION { + label 'process_single' + + conda 'r-ggplot2=3.4.3 bioconductor-shortread=1.58.0 r-ggpubr=0.6.0 r-ggmsa=1.0.2 r-seqmagick=0.1.6 r-tidyr=1.3.0 r-ggseqlogo=0.1 r-cowplot=1.1.1 r-seqinr=4.2_30 r-optparse=1.7.3 r-dplyr=1.1.2 r-plyr=1.8.8 r-stringr=1.5.0 r-plotly=4.10.2' + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/mulled-v2-6de07928379e6eface08a0019c4a1d6b5192e805:0d77388f37ddd923a087f7792e30e83ab54c918c-0' : + 'biocontainers/mulled-v2-6de07928379e6eface08a0019c4a1d6b5192e805:0d77388f37ddd923a087f7792e30e83ab54c918c-0' }" + + input: + path(contrasts) + + output: + path("*.txt"), emit: design_matrix + // path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + + """ + #!/usr/bin/env Rscript + #### author: Laurence Kuhlburger + #### Released under the MIT license. See git repository (https://github.com/nf-core/crisprseq) for full license text. + #### + #### Orient a reference sequence according to reads orientation. + + data <- read.table("$contrasts", header = TRUE, sep = ";", stringsAsFactors = FALSE) + print(data) + # Loop through each row in the data + for (i in 1:nrow(data)) { + # Extract control and treatment samples for the current row + control_samples <- unlist(strsplit(data\$reference[i], ",")) + treatment_samples <- unlist(strsplit(data\$treatment[i], ",")) + + # Create a vector of all unique samples + all_samples <- unique(c(control_samples, treatment_samples)) + + # Initialize a matrix to store the design matrix + design_matrix <- data.frame(matrix(0, nrow = length(all_samples), ncol = 3, + dimnames = list(all_samples, c("Samples", "baseline", paste0(gsub(',', '_', data\$treatment[i] ),"_vs_", data\$reference[i]))))) + + # Set baseline and treatment values in the design matrix + design_matrix[, "Samples"] <- rownames(design_matrix) + design_matrix\$baseline <- 1 + design_matrix[treatment_samples, paste0(gsub(',', '_', data\$treatment[1] ),"_vs_",gsub(",","_",data\$reference[i]))] <- 1 + + # Print the design matrix to a file + output_file <- paste0(gsub(',', '_', data\$treatment[1] ),"_vs_",gsub(",","_",data\$reference[i]),".txt") + write.table(design_matrix, output_file, sep = "\t", quote = FALSE, row.names=FALSE) + } + """ +} diff --git a/nextflow.config b/nextflow.config index 5f6dc691..0f86bcbb 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,11 +16,12 @@ params { protospacer = null library = null crisprcleanr = null - rra_contrasts = null + contrasts = null mle_design_matrix = null count_table = null min_reads = 30 min_targeted_genes = 3 + rra = false bagel_reference_essentials = 'https://raw.githubusercontent.com/hart-lab/bagel/master/CEGv2.txt' bagel_reference_nonessentials = 'https://raw.githubusercontent.com/hart-lab/bagel/master/NEGv1.txt' @@ -199,6 +200,7 @@ profiles { test_screening_full { includeConfig 'conf/test_screening_full.config' } test_screening { includeConfig 'conf/test_screening.config' } test_screening_paired { includeConfig 'conf/test_screening_paired.config' } + test_screening_rra { includeConfig 'conf/test_screening_rra.config' } } // Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile diff --git a/nextflow_schema.json b/nextflow_schema.json index ddc1856e..58a4d67c 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -156,12 +156,16 @@ "exists": true, "description": "Design matrix used for MAGeCK MLE to call essential genes under multiple conditions while considering sgRNA knockout efficiency" }, - "rra_contrasts": { + "contrasts": { "type": "string", "format": "file-path", "exists": true, "description": "Comma-separated file with the conditions to be compared. The first one will be the reference (control)" }, + "rra": { + "type": "boolean", + "description": "Parameter in case MAGeCK RRA should be ran instead of MAGeCK MLE." + }, "count_table": { "type": "string", "format": "file-path", diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 83cd51b9..32b02298 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -24,6 +24,14 @@ if(params.mle_design_matrix) { .set { ch_design } } +if(params.rra && params.mle_design_matrix) { + warning "mle_design_matrix will only be used for the MAGeCK MLE computations" + } + +if(params.rra && !params.contrasts) { + error "Please also provide the contrasts table to compare the samples for MAGeCK RRA" + } + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ CONFIG FILES @@ -66,6 +74,7 @@ include { BAGEL2_FC } from '../modules/local/bagel2/fc' include { BAGEL2_BF } from '../modules/local/bagel2/bf' include { BAGEL2_PR } from '../modules/local/bagel2/pr' include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' +include { MATRICESCREATION } from '../modules/local/matricescreation' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -157,8 +166,8 @@ workflow CRISPRSEQ_SCREENING { }.set { ch_counts } } - if(params.rra_contrasts) { - Channel.fromPath(params.rra_contrasts) + if(params.rra) { + Channel.fromPath(params.contrasts) .splitCsv(header:true, sep:';' ) .set { ch_contrasts } counts = ch_contrasts.combine(ch_counts) @@ -175,8 +184,8 @@ workflow CRISPRSEQ_SCREENING { ch_versions = ch_versions.mix(MAGECK_GRAPHRRA.out.versions) } - if(params.rra_contrasts) { - Channel.fromPath(params.rra_contrasts) + if(params.contrasts && params.rra) { + Channel.fromPath(params.contrasts) .splitCsv(header:true, sep:';' ) .set { ch_bagel } counts = ch_bagel.combine(ch_counts) @@ -216,8 +225,14 @@ workflow CRISPRSEQ_SCREENING { } - if(params.mle_design_matrix) { - ch_mle = ch_counts.combine(ch_design) + if((params.mle_design_matrix) || (params.contrasts && !rra)) { + if(params.mle_design_matrix) { + ch_mle = ch_counts.combine(ch_design) + } + if(params.contrasts) { + MATRICESCREATION(params.contrasts) + ch_mle = ch_counts.combine(MATRICESCREATION.out.design_matrix) + } ch_mle.map { it -> [[id: it[1].getBaseName()], it[0], it[1]] }.set { ch_designed_mle } From 71f06be3772097e7a1f433ab223a9a7631bae2ba Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Tue, 12 Dec 2023 11:04:35 +0100 Subject: [PATCH 002/226] Fix spacing indent --- modules/local/matricescreation.nf | 1 - workflows/crisprseq_screening.nf | 2 +- 2 files changed, 1 insertion(+), 2 deletions(-) diff --git a/modules/local/matricescreation.nf b/modules/local/matricescreation.nf index cb1005ef..6523e722 100644 --- a/modules/local/matricescreation.nf +++ b/modules/local/matricescreation.nf @@ -11,7 +11,6 @@ process MATRICESCREATION { output: path("*.txt"), emit: design_matrix - // path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 32b02298..04313669 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -184,7 +184,7 @@ workflow CRISPRSEQ_SCREENING { ch_versions = ch_versions.mix(MAGECK_GRAPHRRA.out.versions) } - if(params.contrasts && params.rra) { + if(params.contrasts) { Channel.fromPath(params.contrasts) .splitCsv(header:true, sep:';' ) .set { ch_bagel } From 950c07a00e4bf5d8501bd46596a6b0b1a7476925 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Tue, 12 Dec 2023 11:17:12 +0100 Subject: [PATCH 003/226] Remove the fastqc module that was there twice in the workflow --- workflows/crisprseq_screening.nf | 11 ----------- 1 file changed, 11 deletions(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 676f6eda..cdc55549 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -150,17 +150,6 @@ workflow CRISPRSEQ_SCREENING { .last() .set { joined } - // - // MODULE: Run FastQC - // - FASTQC ( - ch_input - ) - - - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - - // // MODULE: Run mageck count From 63bc7f1e581b2b789e77534bf7996308f7a7063c Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Tue, 12 Dec 2023 12:43:56 +0100 Subject: [PATCH 004/226] fix typo --- workflows/crisprseq_screening.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index cdc55549..b8c92aaf 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -246,7 +246,7 @@ workflow CRISPRSEQ_SCREENING { } - if((params.mle_design_matrix) || (params.contrasts && !rra)) { + if((params.mle_design_matrix) || (params.contrasts && !params.rra)) { if(params.mle_design_matrix) { ch_mle = ch_counts.combine(ch_design) } From 0ef0f1ff6014c4230b758426e87a36b8a9d66528 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 18 Dec 2023 11:37:49 +0100 Subject: [PATCH 005/226] bump version dev --- CHANGELOG.md | 3 +++ assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 3 files changed, 6 insertions(+), 3 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ed770355..129234e4 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,9 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). + +## [v2.2.0dev] + ## [v2.1.1 - Jamon Salas - patch](https://github.com/nf-core/crisprseq/releases/tag/2.1.1) - [14.12.2023] ### Added diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index baec67f1..368215a8 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/crisprseq + This report has been generated by the nf-core/crisprseq analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-crisprseq-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index 6dd777af..89349877 100644 --- a/nextflow.config +++ b/nextflow.config @@ -267,7 +267,7 @@ manifest { description = """Pipeline for the analysis of CRISPR data""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '2.1.1' + version = '2.2.0dev' doi = 'https://doi.org/10.5281/zenodo.7598496' } From 23e241a5a50b328b566d90282e233601818192cc Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Tue, 19 Dec 2023 11:41:14 +0100 Subject: [PATCH 006/226] Add function to pass library.txt into library.fasta --- workflows/crisprseq_screening.nf | 38 ++++++++++++++++++++++++++++++-- 1 file changed, 36 insertions(+), 2 deletions(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 7607a4c6..c4e3f8db 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -15,8 +15,38 @@ log.info logo + paramsSummaryLog(workflow) + citation WorkflowCrisprseq.initialise(params, log) +def text_to_fasta(lines) { + String line1 + String sequence1 + String sequences = "" + String id1 + lines.withReader { + while ( line1=it.readLine() ) { + if (line1.size() >= 2) { + def fields = line1.split() + sequence1 = "> ${fields[0]}" + //println(sequence1) + id1 = fields[1] + sequences = sequences + sequence1 + "\n" + id1 + "\n" + } + } + } + return sequences + } + // Set screening parameters and channels -if (params.library) { ch_library = file(params.library) } +if (params.library) { + ch_library = Channel.fromPath(params.library) + ch_library.map { test -> + fasta_line = text_to_fasta(test) + [fasta_line] + }.collectFile(name: "test.fa") + .view() + .set{ ch_fasta } + + ch_fasta.dump(tag: "test") +} + if (params.crisprcleanr) { ch_crisprcleanr = Channel.value(params.crisprcleanr) } if(params.mle_design_matrix) { @@ -67,7 +97,7 @@ include { BAGEL2_FC } from '../modules/local/bagel2/fc' include { BAGEL2_BF } from '../modules/local/bagel2/bf' include { BAGEL2_PR } from '../modules/local/bagel2/pr' include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' - +include { BOWTIE2_BUILD } from '../modules/nf-core/bowtie2/build/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ RUN MAIN WORKFLOW @@ -92,6 +122,10 @@ workflow CRISPRSEQ_SCREENING { .set { ch_input } + ch_text_to_fasta = Channel.of([id: "text to fasta"]) + ch_text_to_fasta.concat(ch_fasta).collect().dump(tag: "DUMPING CHANNEL") + BOWTIE2_BUILD(ch_text_to_fasta.concat(ch_fasta).collect()) + // // MODULE: Run FastQC // From 0aa777d1a09a9c5899ab47c05f77bd05ae374790 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Tue, 19 Dec 2023 16:41:14 +0100 Subject: [PATCH 007/226] Add bowtie alignment --- workflows/crisprseq_screening.nf | 29 +++++++++++++---------------- 1 file changed, 13 insertions(+), 16 deletions(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index c4e3f8db..49bb00b0 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -24,9 +24,8 @@ def text_to_fasta(lines) { while ( line1=it.readLine() ) { if (line1.size() >= 2) { def fields = line1.split() - sequence1 = "> ${fields[0]}" - //println(sequence1) - id1 = fields[1] + sequence1 = "> ${fields[1]}" + id1 = fields[0] sequences = sequences + sequence1 + "\n" + id1 + "\n" } } @@ -34,18 +33,7 @@ def text_to_fasta(lines) { return sequences } -// Set screening parameters and channels -if (params.library) { - ch_library = Channel.fromPath(params.library) - ch_library.map { test -> - fasta_line = text_to_fasta(test) - [fasta_line] - }.collectFile(name: "test.fa") - .view() - .set{ ch_fasta } - - ch_fasta.dump(tag: "test") -} + if (params.crisprcleanr) { ch_crisprcleanr = Channel.value(params.crisprcleanr) } @@ -121,9 +109,18 @@ workflow CRISPRSEQ_SCREENING { return [ meta + [ single_end:fastq_2?false:true ], fastq_2?[ fastq_1, fastq_2 ]:[ fastq_1 ] ] } .set { ch_input } + if (params.library) { + library_file = file(params.library) + ch_library = Channel.fromPath(library_file) + ch_library.map { test -> + fasta_line = text_to_fasta(test) + fasta_line + }.collectFile(name: 'library.fa', newLine: true).set{ ch_fasta } + + } ch_text_to_fasta = Channel.of([id: "text to fasta"]) - ch_text_to_fasta.concat(ch_fasta).collect().dump(tag: "DUMPING CHANNEL") + test = ch_text_to_fasta.concat(ch_fasta).collect() BOWTIE2_BUILD(ch_text_to_fasta.concat(ch_fasta).collect()) // From 9ba2c1b0772be92f7edd28b782a5f2ecb17e75f9 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 19 Dec 2023 19:03:39 +0000 Subject: [PATCH 008/226] Template update for nf-core/tools version 2.11 --- .github/CONTRIBUTING.md | 3 + .github/PULL_REQUEST_TEMPLATE.md | 1 + .github/workflows/ci.yml | 2 +- .github/workflows/fix-linting.yml | 4 +- .github/workflows/linting.yml | 12 +- .gitpod.yml | 4 +- CHANGELOG.md | 2 +- README.md | 17 +-- assets/multiqc_config.yml | 6 +- assets/slackreport.json | 2 +- conf/modules.config | 2 +- docs/usage.md | 4 +- lib/NfcoreTemplate.groovy | 32 ++--- modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 7 ++ .../custom/dumpsoftwareversions/main.nf | 6 +- .../custom/dumpsoftwareversions/meta.yml | 7 +- .../dumpsoftwareversions/tests/main.nf.test | 38 ++++++ .../tests/main.nf.test.snap | 27 +++++ .../dumpsoftwareversions/tests/tags.yml | 2 + modules/nf-core/fastqc/environment.yml | 7 ++ modules/nf-core/fastqc/main.nf | 10 +- modules/nf-core/fastqc/meta.yml | 5 + modules/nf-core/fastqc/tests/main.nf.test | 109 ++++++++++++++++++ .../nf-core/fastqc/tests/main.nf.test.snap | 10 ++ modules/nf-core/fastqc/tests/tags.yml | 2 + modules/nf-core/multiqc/environment.yml | 7 ++ modules/nf-core/multiqc/main.nf | 8 +- modules/nf-core/multiqc/meta.yml | 11 +- modules/nf-core/multiqc/tests/main.nf.test | 63 ++++++++++ modules/nf-core/multiqc/tests/tags.yml | 2 + nextflow.config | 12 +- 32 files changed, 363 insertions(+), 67 deletions(-) create mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml create mode 100644 modules/nf-core/fastqc/environment.yml create mode 100644 modules/nf-core/fastqc/tests/main.nf.test create mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastqc/tests/tags.yml create mode 100644 modules/nf-core/multiqc/environment.yml create mode 100644 modules/nf-core/multiqc/tests/main.nf.test create mode 100644 modules/nf-core/multiqc/tests/tags.yml diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 212cbdbe..3de1bef3 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -27,6 +27,9 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests +You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to +receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. + When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 22f5beff..1ae44009 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -19,6 +19,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/cris - [ ] If necessary, also make a PR on the nf-core/crisprseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. - [ ] `CHANGELOG.md` is updated. diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 277385ff..fbb59dab 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,7 +28,7 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index d5db7392..2007e653 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -13,7 +13,7 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 with: token: ${{ secrets.nf_core_bot_auth_token }} @@ -24,7 +24,7 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install Prettier run: npm install -g prettier @prettier/plugin-php diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index b8bdd214..905c58e4 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,9 +14,9 @@ jobs: EditorConfig: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install editorconfig-checker run: npm install -g editorconfig-checker @@ -27,9 +27,9 @@ jobs: Prettier: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install Prettier run: npm install -g prettier @@ -40,7 +40,7 @@ jobs: PythonBlack: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - name: Check code lints with Black uses: psf/black@stable @@ -71,7 +71,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 diff --git a/.gitpod.yml b/.gitpod.yml index 25488dcc..acf72695 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -4,7 +4,9 @@ tasks: command: | pre-commit install --install-hooks nextflow self-update - + - name: unset JAVA_TOOL_OPTIONS + command: | + unset JAVA_TOOL_OPTIONS vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/CHANGELOG.md b/CHANGELOG.md index d48d4ecb..4bfca101 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.1.0dev - [date] +## v2.1.1 - [date] Initial release of nf-core/crisprseq, created with the [nf-core](https://nf-co.re/) template. diff --git a/README.md b/README.md index d10e9a88..4e25b29c 100644 --- a/README.md +++ b/README.md @@ -30,11 +30,8 @@ ## Usage -:::note -If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how -to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) -with `-profile test` before running the workflow on actual data. -::: +> [!NOTE] +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. - + diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index b14d4038..e7d12291 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,9 @@ report_comment: > - This report has been generated by the nf-core/crisprseq + + This report has been generated by the nf-core/crisprseq analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. + report_section_order: "nf-core-crisprseq-methods-description": order: -1000 diff --git a/assets/slackreport.json b/assets/slackreport.json index a3cdd5e4..5206f763 100644 --- a/assets/slackreport.json +++ b/assets/slackreport.json @@ -3,7 +3,7 @@ { "fallback": "Plain-text summary of the attachment.", "color": "<% if (success) { %>good<% } else { %>danger<%} %>", - "author_name": "nf-core/crisprseq v${version} - ${runName}", + "author_name": "nf-core/crisprseq ${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/conf/modules.config b/conf/modules.config index 39e81386..d91c6aba 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -39,7 +39,7 @@ process { } withName: 'MULTIQC' { - ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' + ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ path: { "${params.outdir}/multiqc" }, mode: params.publish_dir_mode, diff --git a/docs/usage.md b/docs/usage.md index 6ce5fadc..2247d5ba 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -20,7 +20,7 @@ You will need to create a samplesheet with information about the samples you wou The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz @@ -33,7 +33,7 @@ The pipeline will auto-detect whether a sample is single- or paired-end using th A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index 01b8653d..e248e4c3 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -4,6 +4,7 @@ import org.yaml.snakeyaml.Yaml import groovy.json.JsonOutput +import nextflow.extension.FilesEx class NfcoreTemplate { @@ -141,12 +142,14 @@ class NfcoreTemplate { try { if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } [ 'sendmail', '-t' ].execute() << sendmail_html log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" } catch (all) { // Catch failures and try with plaintext def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { mail_cmd += [ '-A', mqc_report ] } mail_cmd.execute() << email_html @@ -155,14 +158,16 @@ class NfcoreTemplate { } // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() } // @@ -227,15 +232,14 @@ class NfcoreTemplate { // Dump pipeline parameters in a json file // public static void dump_parameters(workflow, params) { - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def output_pf = new File(output_d, "params_${timestamp}.json") + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") def jsonStr = JsonOutput.toJson(params) - output_pf.text = JsonOutput.prettyPrint(jsonStr) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() } // diff --git a/modules.json b/modules.json index 24fe422e..b220a458 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "bd8092b67b5103bdd52e300f75889442275c3117", + "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "4ab13872435962dadc239979554d13709e20bf29", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml new file mode 100644 index 00000000..f0c63f69 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -0,0 +1,7 @@ +name: custom_dumpsoftwareversions +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.17 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index ebc87273..7685b33c 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : + 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml index c32657de..5f15a5fd 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -1,4 +1,4 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: custom_dumpsoftwareversions description: Custom module used to dump software versions within the nf-core pipeline template keywords: @@ -16,7 +16,6 @@ input: type: file description: YML file containing software versions pattern: "*.yml" - output: - yml: type: file @@ -30,7 +29,9 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@drpatelh" - "@grst" +maintainers: + - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test new file mode 100644 index 00000000..eec1db10 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -0,0 +1,38 @@ +nextflow_process { + + name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" + script "../main.nf" + process "CUSTOM_DUMPSOFTWAREVERSIONS" + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "dumpsoftwareversions" + tag "custom/dumpsoftwareversions" + + test("Should run without failures") { + when { + process { + """ + def tool1_version = ''' + TOOL1: + tool1: 0.11.9 + '''.stripIndent() + + def tool2_version = ''' + TOOL2: + tool2: 1.9 + '''.stripIndent() + + input[0] = Channel.of(tool1_version, tool2_version).collectFile() + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap new file mode 100644 index 00000000..4274ed57 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -0,0 +1,27 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ], + "1": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "2": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "mqc_yml": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "versions": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "yml": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ] + } + ], + "timestamp": "2023-11-03T14:43:22.157011" + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml new file mode 100644 index 00000000..405aa24a --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml @@ -0,0 +1,2 @@ +custom/dumpsoftwareversions: + - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml new file mode 100644 index 00000000..1787b38a --- /dev/null +++ b/modules/nf-core/fastqc/environment.yml @@ -0,0 +1,7 @@ +name: fastqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 249f9064..9e19a74c 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -2,10 +2,10 @@ process FASTQC { tag "$meta.id" label 'process_medium' - conda "bioconda::fastqc=0.11.9" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' : - 'biocontainers/fastqc:0.11.9--0' }" + 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : + 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" input: tuple val(meta), path(reads) @@ -37,7 +37,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ @@ -49,7 +49,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ } diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml index 4da5bb5a..ee5507e0 100644 --- a/modules/nf-core/fastqc/meta.yml +++ b/modules/nf-core/fastqc/meta.yml @@ -50,3 +50,8 @@ authors: - "@grst" - "@ewels" - "@FelixKrueger" +maintainers: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test new file mode 100644 index 00000000..b9e8f926 --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -0,0 +1,109 @@ +nextflow_process { + + name "Test Process FASTQC" + script "../main.nf" + process "FASTQC" + tag "modules" + tag "modules_nfcore" + tag "fastqc" + + test("Single-Read") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id: 'test', single_end:true ], + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:
Mon 2 Oct 2023
test.gz
+ // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, + { assert path(process.out.html.get(0).get(1)).getText().contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + ) + } + } +// TODO +// // +// // Test with paired-end data +// // +// workflow test_fastqc_paired_end { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with interleaved data +// // +// workflow test_fastqc_interleaved { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with bam data +// // +// workflow test_fastqc_bam { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with multiple samples +// // +// workflow test_fastqc_multiple { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with custom prefix +// // +// workflow test_fastqc_custom_prefix { +// input = [ +// [ id:'mysample', single_end:true ], // meta map +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } +} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap new file mode 100644 index 00000000..636a32ce --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -0,0 +1,10 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2023-10-09T23:40:54+0000" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml new file mode 100644 index 00000000..7834294b --- /dev/null +++ b/modules/nf-core/fastqc/tests/tags.yml @@ -0,0 +1,2 @@ +fastqc: + - modules/nf-core/fastqc/** diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml new file mode 100644 index 00000000..bc0bdb5b --- /dev/null +++ b/modules/nf-core/multiqc/environment.yml @@ -0,0 +1,7 @@ +name: multiqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.18 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1fc387be..00cc48d2 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -1,10 +1,10 @@ process MULTIQC { label 'process_single' - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : + 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -25,12 +25,14 @@ process MULTIQC { def args = task.ext.args ?: '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' + def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' """ multiqc \\ --force \\ $args \\ $config \\ $extra_config \\ + $logo \\ . cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f93b5ee5..f1aa660e 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,5 +1,5 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json -name: MultiQC +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: - QC @@ -13,7 +13,6 @@ tools: homepage: https://multiqc.info/ documentation: https://multiqc.info/docs/ licence: ["GPL-3.0-or-later"] - input: - multiqc_files: type: file @@ -31,7 +30,6 @@ input: type: file description: Optional logo file for MultiQC pattern: "*.{png}" - output: - report: type: file @@ -54,3 +52,8 @@ authors: - "@bunop" - "@drpatelh" - "@jfy133" +maintainers: + - "@abhi18av" + - "@bunop" + - "@drpatelh" + - "@jfy133" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test new file mode 100644 index 00000000..c2dad217 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -0,0 +1,63 @@ +nextflow_process { + + name "Test Process MULTIQC" + script "../main.nf" + process "MULTIQC" + tag "modules" + tag "modules_nfcore" + tag "multiqc" + + test("MULTIQC: FASTQC") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } + + test("MULTIQC: FASTQC and a config file") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } +} diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml new file mode 100644 index 00000000..bea6c0d3 --- /dev/null +++ b/modules/nf-core/multiqc/tests/tags.yml @@ -0,0 +1,2 @@ +multiqc: + - modules/nf-core/multiqc/** diff --git a/nextflow.config b/nextflow.config index 8f99598f..5a70b423 100644 --- a/nextflow.config +++ b/nextflow.config @@ -14,7 +14,7 @@ params { input = null // References genome = null - igenomes_base = 's3://ngi-igenomes/igenomes' + igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false @@ -82,6 +82,7 @@ profiles { dumpHashes = true process.beforeScript = 'echo $HOSTNAME' cleanup = false + nextflow.enable.configProcessNamesValidation = true } conda { conda.enabled = true @@ -104,13 +105,13 @@ profiles { } docker { docker.enabled = true - docker.userEmulation = true conda.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false + runOptions = '-u $(id -u):$(id -g)' } arm { docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' @@ -181,7 +182,7 @@ singularity.registry = 'quay.io' // Nextflow plugins plugins { - id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet } // Load igenomes.config if required @@ -204,6 +205,9 @@ env { // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] +// Disable process selector warnings by default. Use debug profile to enable warnings. +nextflow.enable.configProcessNamesValidation = false + def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true @@ -229,7 +233,7 @@ manifest { description = """Pipeline for the analysis of CRISPR data""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '2.1.0dev' + version = '2.1.1' doi = '' } From 64dd96c4edaf09540297c76c20392368ccb0269c Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 20 Dec 2023 15:55:47 +0000 Subject: [PATCH 009/226] Template update for nf-core/tools version 2.11.1 --- .../{release-announcments.yml => release-announcements.yml} | 0 nextflow.config | 4 ++-- 2 files changed, 2 insertions(+), 2 deletions(-) rename .github/workflows/{release-announcments.yml => release-announcements.yml} (100%) diff --git a/.github/workflows/release-announcments.yml b/.github/workflows/release-announcements.yml similarity index 100% rename from .github/workflows/release-announcments.yml rename to .github/workflows/release-announcements.yml diff --git a/nextflow.config b/nextflow.config index 5a70b423..37b455f2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -111,10 +111,10 @@ profiles { shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false - runOptions = '-u $(id -u):$(id -g)' + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { singularity.enabled = true From a9fa4b9f03046ba4caa36c6e50197bee6dd9d59f Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 21 Dec 2023 13:19:33 +0000 Subject: [PATCH 010/226] [automated] Fix linting with Prettier --- assets/multiqc_config.yml | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 32a68e14..f39ea3b8 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,9 +1,9 @@ report_comment: > - + This report has been generated by the nf-core/crisprseq analysis pipeline. For information about how to interpret these results, please see the documentation. - + report_section_order: "nf-core-crisprseq-methods-description": order: -1000 From 292da8c0f41d5443dcdf50af44cbc295cd28e6b1 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Thu, 21 Dec 2023 13:26:43 +0000 Subject: [PATCH 011/226] update fastqc and multiqc --- modules.json | 4 +- modules/nf-core/fastqc/tests/main.nf.test | 68 +++++++++++++++++++++++ modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- 4 files changed, 73 insertions(+), 5 deletions(-) diff --git a/modules.json b/modules.json index 0adbc8ec..582acec2 100644 --- a/modules.json +++ b/modules.json @@ -49,7 +49,7 @@ }, "fastqc": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", "installed_by": ["modules"] }, "mageck/count": { @@ -89,7 +89,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "214d575774c172062924ad3564b4f66655600730", + "git_sha": "4ab13872435962dadc239979554d13709e20bf29", "installed_by": ["modules"] }, "pear": { diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 6437a144..b9e8f926 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -38,4 +38,72 @@ nextflow_process { ) } } +// TODO +// // +// // Test with paired-end data +// // +// workflow test_fastqc_paired_end { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with interleaved data +// // +// workflow test_fastqc_interleaved { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with bam data +// // +// workflow test_fastqc_bam { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with multiple samples +// // +// workflow test_fastqc_multiple { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with custom prefix +// // +// workflow test_fastqc_custom_prefix { +// input = [ +// [ id:'mysample', single_end:true ], // meta map +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } } diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index d2a9f21a..bc0bdb5b 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.17 + - bioconda::multiqc=1.18 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 0f6e3f62..00cc48d2 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : + 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" From d4680d4d85279c6c5ec9db9fee589486265a85c5 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Wed, 3 Jan 2024 08:41:25 +0100 Subject: [PATCH 012/226] Update workflows/crisprseq_screening.nf MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Júlia Mir Pedrol --- workflows/crisprseq_screening.nf | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 49bb00b0..996e0c71 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -113,8 +113,7 @@ workflow CRISPRSEQ_SCREENING { library_file = file(params.library) ch_library = Channel.fromPath(library_file) ch_library.map { test -> - fasta_line = text_to_fasta(test) - fasta_line + text_to_fasta(test) }.collectFile(name: 'library.fa', newLine: true).set{ ch_fasta } } From 84ec7bdd9548b9f516e0338a4bade875dffb9d74 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Wed, 3 Jan 2024 08:41:35 +0100 Subject: [PATCH 013/226] Update workflows/crisprseq_screening.nf MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Júlia Mir Pedrol --- workflows/crisprseq_screening.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 996e0c71..dcf198e5 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -114,7 +114,7 @@ workflow CRISPRSEQ_SCREENING { ch_library = Channel.fromPath(library_file) ch_library.map { test -> text_to_fasta(test) - }.collectFile(name: 'library.fa', newLine: true).set{ ch_fasta } + }.collectFile(name: 'library.fa', newLine: true).set{ ch_fasta } } From 6c340f20b59a82de9304543ec48d420037bb3bb1 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 10 Jan 2024 08:34:45 +0000 Subject: [PATCH 014/226] [automated] Fix linting with Prettier --- CHANGELOG.md | 1 - 1 file changed, 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 129234e4..52d8c949 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,6 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). - ## [v2.2.0dev] ## [v2.1.1 - Jamon Salas - patch](https://github.com/nf-core/crisprseq/releases/tag/2.1.1) - [14.12.2023] From 34cf80d6b13b5d5ec45cfaa8a7b6ad531d8117ac Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Wed, 10 Jan 2024 09:15:29 +0000 Subject: [PATCH 015/226] update changelog --- CHANGELOG.md | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 52d8c949..3c7b6bb3 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -5,6 +5,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## [v2.2.0dev] +### Added + +- Template update to 2.11.1 ([#105](https://github.com/nf-core/crisprseq/pull/105)) + ## [v2.1.1 - Jamon Salas - patch](https://github.com/nf-core/crisprseq/releases/tag/2.1.1) - [14.12.2023] ### Added From 224e52dfca4e6d20810db25a5bb3ae3b7c339c1c Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Wed, 10 Jan 2024 09:22:03 +0000 Subject: [PATCH 016/226] update multiqc_config.yml according to the template --- assets/multiqc_config.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 368215a8..1e3b44b6 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,5 +1,5 @@ report_comment: > - This report has been generated by the nf-core/crisprseq + This report has been generated by the nf-core/crisprseq analysis pipeline. For information about how to interpret these results, please see the documentation. report_section_order: From 3fd7819b2a51e1d5a65d5e04d8eb8490f78514c3 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 15 Jan 2024 13:18:40 +0100 Subject: [PATCH 017/226] work on bowtie --- nextflow_schema.json | 11 +++-- workflows/crisprseq_screening.nf | 85 ++++++++++++++++++-------------- 2 files changed, 57 insertions(+), 39 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index c2da2600..a2ac961f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -190,12 +190,12 @@ "min_reads": { "type": "number", "description": "a filter threshold value for sgRNAs, based on their average counts in the control sample", - "default": 30.0 + "default": 30 }, "min_targeted_genes": { "type": "number", "description": "Minimal number of different genes targeted by sgRNAs in a biased segment in order for the corresponding counts to be corrected for CRISPRcleanR", - "default": 3.0 + "default": 3 }, "bagel_reference_essentials": { "type": "string", @@ -477,5 +477,10 @@ { "$ref": "#/definitions/generic_options" } - ] + ], + "properties": { + "fasta": { + "type": "string" + } + } } diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index dcf198e5..08fdb460 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -86,6 +86,8 @@ include { BAGEL2_BF } from '../modules/local/bagel2/bf' include { BAGEL2_PR } from '../modules/local/bagel2/pr' include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' include { BOWTIE2_BUILD } from '../modules/nf-core/bowtie2/build/main' +include { BOWTIE2_ALIGN } from '../modules/nf-core/bowtie2/align/main' + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ RUN MAIN WORKFLOW @@ -101,42 +103,45 @@ workflow CRISPRSEQ_SCREENING { if(!params.count_table){ // - // Create input channel from input file provided through params.input + // If bowtie aligner is used, convert the library in text format to a fasta file // - Channel.fromSamplesheet("input") - .map{ meta, fastq_1, fastq_2, x, y, z -> - // x (reference), y (protospacer), and z (template) are part of the targeted workflows and we don't need them - return [ meta + [ single_end:fastq_2?false:true ], fastq_2?[ fastq_1, fastq_2 ]:[ fastq_1 ] ] } - .set { ch_input } - if (params.library) { - library_file = file(params.library) - ch_library = Channel.fromPath(library_file) - ch_library.map { test -> - text_to_fasta(test) - }.collectFile(name: 'library.fa', newLine: true).set{ ch_fasta } - + ch_library = Channel.fromPath(params.library) + ch_library.map { line -> + fasta_line = text_to_fasta(line) + fasta_line + }.collectFile(name: 'library.fa', newLine: true).set{ ch_fasta } } - ch_text_to_fasta = Channel.of([id: "text to fasta"]) - test = ch_text_to_fasta.concat(ch_fasta).collect() + if(params.fasta){ ch_fasta = Channel.fromPath(params.fasta) } + + // create bowtie index BOWTIE2_BUILD(ch_text_to_fasta.concat(ch_fasta).collect()) + //ch_versions = ch_versions.mix(BOWTIE2_BUILD.out.versions) // - // MODULE: Run FastQC + // Create input channel from input file provided through params.input // - FASTQC ( - ch_input - ) - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + Channel.fromSamplesheet("input") + .map{ meta, fastq_1, fastq_2, x, y, z -> + // x (reference), y (protospacer), and z (template) are part of the targeted workflows and we don't need them + return [ meta + [ single_end:fastq_2?false:true ], fastq_2?[ fastq_1, fastq_2 ]:[ fastq_1 ] ] } + .set { ch_input } + ch_input.dump(tag: "ch_input") + BOWTIE2_ALIGN ( + ch_input, + BOWTIE2_BUILD.out.index, + false, + false + ) ch_input_cutadapt = ch_input.combine(Channel.value([[]])) - if(params.cutadapt) { - CUTADAPT( - ch_input_cutadapt - ) + if(params.cutadapt) { + CUTADAPT( + ch_input_cutadapt + ) ch_versions = ch_versions.mix(CUTADAPT.out.versions) CUTADAPT.out.reads @@ -146,6 +151,14 @@ workflow CRISPRSEQ_SCREENING { .set { ch_input } } + // + // MODULE: Run FastQC + // + FASTQC ( + ch_input + ) + ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + // this is to concatenate everything for mageck count ch_input @@ -280,9 +293,9 @@ workflow CRISPRSEQ_SCREENING { } - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique{ it.text }.collectFile(name: 'collated_versions.yml') - ) + //CUSTOM_DUMPSOFTWAREVERSIONS ( + // ch_versions.unique{ it.text }.collectFile(name: 'collated_versions.yml') + //) // // MODULE: MultiQC @@ -296,21 +309,21 @@ workflow CRISPRSEQ_SCREENING { ch_multiqc_files = Channel.empty() ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) + //ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) if(!params.count_table) { ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) } else { ch_multiqc_files = channel.empty() } - MULTIQC ( - ch_multiqc_files.collect(), - ch_multiqc_config.collect().ifEmpty([]), - ch_multiqc_custom_config.collect().ifEmpty([]), - ch_multiqc_logo.collect().ifEmpty([]) - ) - multiqc_report = MULTIQC.out.report.toList() - ch_versions = ch_versions.mix(MULTIQC.out.versions) + //MULTIQC ( + // ch_multiqc_files.collect(), + // ch_multiqc_config.collect().ifEmpty([]), + //ch_multiqc_custom_config.collect().ifEmpty([]), + //ch_multiqc_logo.collect().ifEmpty([]) + //) + //multiqc_report = MULTIQC.out.report.toList() + //h_versions = ch_versions.mix(MULTIQC.out.versions) } /* From 32493db4d2835b3a039970de09c9a4555ab99ee5 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 15 Jan 2024 13:47:10 +0100 Subject: [PATCH 018/226] Update nextflow_schema.json MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Júlia Mir Pedrol --- nextflow_schema.json | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 9e8d5fd9..0fc99468 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -164,7 +164,8 @@ }, "rra": { "type": "boolean", - "description": "Parameter in case MAGeCK RRA should be ran instead of MAGeCK MLE." + "description": "Parameter indicating if MAGeCK RRA should be ran instead of MAGeCK MLE.", + "default": false }, "count_table": { "type": "string", From f6f539c4d00ef0aeeda75ecb9a831866ced13352 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 15 Jan 2024 13:48:05 +0100 Subject: [PATCH 019/226] Update modules/local/matricescreation.nf --- modules/local/matricescreation.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/modules/local/matricescreation.nf b/modules/local/matricescreation.nf index 6523e722..43867fa6 100644 --- a/modules/local/matricescreation.nf +++ b/modules/local/matricescreation.nf @@ -25,7 +25,6 @@ process MATRICESCREATION { #### Orient a reference sequence according to reads orientation. data <- read.table("$contrasts", header = TRUE, sep = ";", stringsAsFactors = FALSE) - print(data) # Loop through each row in the data for (i in 1:nrow(data)) { # Extract control and treatment samples for the current row From 3cb4046b07582e3d24566e61a8dc8d332a29b4bf Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 15 Jan 2024 14:34:01 +0100 Subject: [PATCH 020/226] Update documentation --- CHANGELOG.md | 2 ++ docs/usage/screening.md | 40 ++++++++++++++++++++++++++-------------- 2 files changed, 28 insertions(+), 14 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 3613a432..5d959c27 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,6 +8,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Added - Update all modules to the last version in nf-core/modules ([#92](https://github.com/nf-core/crisprseq/pull/92)) +- More documentation for screening analysis. ([#99](https://github.com/nf-core/crisprseq/pull/99)) +- Contrasts are now given under a different flag and MAGeCK MLE and BAGEL2 are automatically run instead of MAGeCK RRA. ([#99](https://github.com/nf-core/crisprseq/pull/99)) ## [v2.1.0 - Jamon Salas](https://github.com/nf-core/crisprseq/releases/tag/2.1.0) - [14.11.2023] diff --git a/docs/usage/screening.md b/docs/usage/screening.md index 34325513..18fcf36d 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -21,6 +21,7 @@ nextflow run nf-core/crisprseq --analysis screening --input samplesheet.csv --li ``` The following required parameters are here described. + If you wish to input a raw count or normalized table, you can skip the samplesheet parameter as well as the library one and directly input your table using count_table `--count_table your_count_table`. If your count table is normalized, be sure to set the normalization method to none in MAGeCK MLE or MAGeCK RRA using a config file. ### Full samplesheet @@ -41,16 +42,29 @@ SRR8983580,SRR8983580.small.fastq.gz,,treatment An [example samplesheet](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/samplesheet_test.csv) has been provided with the pipeline. + +### cutadapt + +MAGeCK count which is the main alignment software used is normally able to automatically determine the trimming length and sgRNA length, in most cases. Therefore, you don't need to go to this step unless MAGeCK fails to do so by itself. If the nucleotide length in front of sgRNA varies between different reads, you can use cutadapt to remove the adaptor sequences by using the flag `--cutadapt ADAPTER`. + ### library If you are running the pipeline with fastq files and wish to obtain a count table, the library parameter is needed. The library table has three mandatory columns : id, target transcript (or gRNA sequence) and gene symbol. An [example](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/brunello_target_sequence.txt) has been provided with the pipeline. Many libraries can be found on [addgene](https://www.addgene.org/). -After the alignment step, the pipeline currently supports 3 algorithms to detect gene essentiality, MAGeCK rra, MAGeCK mle and BAGEL2. MAGeCK MLE (Maximum Likelihood Estimation) and MAGeCK RRA (Robust Ranking Aggregation) are two different methods provided by the MAGeCK software package to analyze CRISPR-Cas9 screens. BAGEL2 identifies gene essentiality through Bayesian Analysis. +After the alignment step, if you are performing KO (Knock-Out) screens, you can choose to correction of gene independent cell responses to CRISPR-cas9 targeting using crisprcleanr. If you are performing a CRISPR interference or activation screen, this step is not needed. -### MAGeCK rra +The pipeline currently supports 3 algorithms to detect gene essentiality, MAGeCK rra, MAGeCK mle and BAGEL2. MAGeCK MLE (Maximum Likelihood Estimation) and MAGeCK RRA (Robust Ranking Aggregation) are two different methods provided by the MAGeCK software package to analyze CRISPR-Cas9 screens. BAGEL2 identifies gene essentiality through Bayesian Analysis. +We recommend to run MAGeCK MLE and BAGEL2 as these are the most used and most recent algorithms to determine gene essentiality. + +### Running CRISPRcleanR + +CRISPRcleanR is used for gene count normalization and the removal of biases for genomic segments for which copy numbers are amplified. Currently, the pipeline only supports annotation libraries already present in the R package and which can be found [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). -MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, `--rra_contrasts` contains two columns : treatment and reference. These two columns should be separated with a dot comma (;) and contain the `csv` extension. You can also integrate several samples/conditions by comma separating them. Please find an example here below : + +### Running MAGeCK MLE and BAGEL2 with a contrast file + +To run both MAGeCK MLE and BAGEL2, you can provide a contrast file with the flag `--contrasts` with the mandatory headers "treatment" and "reference". These two columns should be separated with a dot comma (;) and contain the `csv` extension. You can also integrate several samples/conditions by comma separating them in each column. Please find an example here below : | reference | treatment | | ----------------- | --------------------- | @@ -59,24 +73,22 @@ MAGeCK RRA performs robust ranking aggregation to identify genes that are consis A full example can be found [here](https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/full_test/samplesheet_full.csv). -### cutadapt +### Running MAGeCK RRA only -MAGeCK is normally able to automatically determine the trimming length and sgRNA length, in most cases. Therefore, you don't need to go to this step unless MAGeCK fails to do so by itself. If the nucleotide length in front of sgRNA varies between different reads, you can use cutadapt to remove the adaptor sequences by using the flag `--cutadapt ADAPTER`. +MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section and also specify `--rra` . -### MAGeCK mle +### Running MAGeCK MLE only -MAGeCK MLE uses a maximum likelihood estimation approach to estimate the effects of gene knockout on cell fitness. It models the read count data of guide RNAs targeting each gene and estimates the dropout probability for each gene. MAGeCK mle requires a design matrix. The design matrix is a `txt` file indicating the effects of different conditions on different samples. -An [example design matrix](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/design_matrix.txt) has been provided with the pipeline. +If you wish to run MAGeCK MLE only, you can specify several design matrices (where you state which comparisons you wish to run) with the flag `--mle_design_matrix`. +MAGeCK MLE uses a maximum likelihood estimation approach to estimate the effects of gene knockout on cell fitness. It models the read count data of guide RNAs targeting each gene and estimates the dropout probability for each gene. +MAGeCK MLE requires one or several design matrices. The design matrix is a `txt` file indicating the effects of different conditions on different samples. +An [example design matrix](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/design_matrix.txt) has been provided with the pipeline. The row names need to match the condition stated in the sample sheet. If there are several designs to be run, you can input a folder containing all the design matrices. The output results will automatically take the name of the design matrix, so make sure you give a meaningful name to the file, for instance "Drug_vs_control.txt". -### Running CRISPRcleanR - -CRISPRcleanR is used for gene count normalization and the removal of biases for genomic segments for which copy numbers are amplified. Currently, the pipeline only supports annotation libraries already present in the R package and which can be found [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). - -### BAGEL2 +### Running BAGEL2 BAGEL2 (Bayesian Analysis of Gene Essentiality with Location) is a computational tool developed by the Hart Lab at Harvard University. It is designed for analyzing large-scale genetic screens, particularly CRISPR-Cas9 screens, to identify genes that are essential for the survival or growth of cells under different conditions. BAGEL2 integrates information about the location of guide RNAs within a gene and leverages this information to improve the accuracy of gene essentiality predictions. -BAGEL2 uses the same contrasts from `--rra_contrasts`. +BAGEL2 uses the same contrasts from `--contrasts`. Note that the pipeline will create the following files in your working directory: From 19fb34c8a6da86e2f90e6595c8758bc8a9077284 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 15 Jan 2024 15:14:04 +0100 Subject: [PATCH 021/226] commit --- modules/local/matricescreation.nf | 2 +- workflows/crisprseq_screening.nf | 34 +++++++++++++++++++++++-------- 2 files changed, 26 insertions(+), 10 deletions(-) diff --git a/modules/local/matricescreation.nf b/modules/local/matricescreation.nf index 43867fa6..40a7efa4 100644 --- a/modules/local/matricescreation.nf +++ b/modules/local/matricescreation.nf @@ -22,7 +22,7 @@ process MATRICESCREATION { #### author: Laurence Kuhlburger #### Released under the MIT license. See git repository (https://github.com/nf-core/crisprseq) for full license text. #### - #### Orient a reference sequence according to reads orientation. + #### Create design matrices data <- read.table("$contrasts", header = TRUE, sep = ";", stringsAsFactors = FALSE) # Loop through each row in the data diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index b8c92aaf..00f77b53 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -17,7 +17,13 @@ WorkflowCrisprseq.initialise(params, log) // Set screening parameters and channels if (params.library) { ch_library = file(params.library) } -if (params.crisprcleanr) { ch_crisprcleanr = Channel.value(params.crisprcleanr) } +if (params.crisprcleanr) { + if(params.crisprcleanr.endsWith(".csv")) { + ch_crisprcleanr_file = file(params.crisprcleanr) + } else { + ch_crisprcleanr_value = Channel.value(params.crisprcleanr) + } + } if(params.mle_design_matrix) { Channel.fromPath(params.mle_design_matrix) @@ -170,14 +176,25 @@ workflow CRISPRSEQ_SCREENING { .set { ch_counts } } - if(params.crisprcleanr) { - ch_crispr_normalize = Channel.of([id: "count_table_normalize"]) - CRISPRCLEANR_NORMALIZE( - ch_crispr_normalize.concat(ch_counts,ch_crisprcleanr).collect(), - params.min_reads, - params.min_targeted_genes - ) + ch_crispr_normalize = Channel.of([id: "count_table_normalize"]).concat(ch_counts) + + if(params.crisprcleanr.endsWith(".csv")) { + CRISPRCLEANR_NORMALIZE( + ch_crispr_normalize.collect(), + ch_crisprcleanr_file, + params.min_reads, + params.min_targeted_genes + ) } else + { + ch_crispr_normalize = Channel.of([id: "count_table_normalize"]).concat(ch_counts) + CRISPRCLEANR_NORMALIZE( + ch_crispr_normalize.collect(), + ch_crisprcleanr_value, + Channel.empty(), + params.min_reads, + params.min_targeted_genes) + } ch_versions = ch_versions.mix(CRISPRCLEANR_NORMALIZE.out.versions) @@ -264,7 +281,6 @@ workflow CRISPRSEQ_SCREENING { ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) - } CUSTOM_DUMPSOFTWAREVERSIONS ( From 77dd701efdf3e15a37a1d999b28198b2b3ea3712 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Tue, 16 Jan 2024 08:08:13 +0000 Subject: [PATCH 022/226] fix version reporting --- modules/local/bagel2/graph.nf | 4 ++-- modules/local/bagel2/pr.nf | 6 +++--- 2 files changed, 5 insertions(+), 5 deletions(-) diff --git a/modules/local/bagel2/graph.nf b/modules/local/bagel2/graph.nf index 32c96046..d7aad230 100644 --- a/modules/local/bagel2/graph.nf +++ b/modules/local/bagel2/graph.nf @@ -64,8 +64,8 @@ process BAGEL2_GRAPH { # alas, no `pyyaml` pre-installed in the cellranger container with open("versions.yml", "w") as f: f.write('"${task.process}":\\n') - f.write(f' pandas: "{version}"\\n') - f.write(f' matplotlib.pyplot: "{matplotlib_version}"\\n') + f.write(f' pandas: {version}\\n') + f.write(f' matplotlib.pyplot: {matplotlib_version}\\n') """ diff --git a/modules/local/bagel2/pr.nf b/modules/local/bagel2/pr.nf index e27366d6..e2290ede 100644 --- a/modules/local/bagel2/pr.nf +++ b/modules/local/bagel2/pr.nf @@ -26,9 +26,9 @@ process BAGEL2_PR { BAGEL.py pr -i $bf -o '${meta.treatment}_vs_${meta.reference}.pr' -e $reference_essentials -n $reference_nonessentials $args cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - BAGEL2: \$( BAGEL.py version | grep -o 'Version: [0-9.]*' | awk '{print \$2}' | grep -v '^\$') + "${task.process}": + python: \$(python --version | sed 's/Python //g') + BAGEL2: \$( BAGEL.py version | grep -o 'Version: [0-9.]*' | awk '{print \$2}' | grep -v '^\$') END_VERSIONS """ From 2877b97bda3629a999e2ec460706e28ed82b6728 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Tue, 16 Jan 2024 08:12:12 +0000 Subject: [PATCH 023/226] run prettier --- CHANGELOG.md | 3 +-- docs/usage/screening.md | 6 ++---- 2 files changed, 3 insertions(+), 6 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index f328a8df..66fcc36f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -14,7 +14,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Added - Update all modules to the last version in nf-core/modules ([#92](https://github.com/nf-core/crisprseq/pull/92)) -- More documentation for screening analysis. ([#99](https://github.com/nf-core/crisprseq/pull/99)) +- More documentation for screening analysis. ([#99](https://github.com/nf-core/crisprseq/pull/99)) - Contrasts are now given under a different flag and MAGeCK MLE and BAGEL2 are automatically run instead of MAGeCK RRA. ([#99](https://github.com/nf-core/crisprseq/pull/99)) - Added cutadapt for screening analysis ([#95](https://github.com/nf-core/crisprseq/pull/95)) @@ -22,7 +22,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Fixed paired-end for screening analysis ([#94](https://github.com/nf-core/crisprseq/pull/94)) - ## [v2.1.0 - Jamon Salas](https://github.com/nf-core/crisprseq/releases/tag/2.1.0) - [14.11.2023] ### Added diff --git a/docs/usage/screening.md b/docs/usage/screening.md index 18fcf36d..b0c6dcac 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -21,7 +21,7 @@ nextflow run nf-core/crisprseq --analysis screening --input samplesheet.csv --li ``` The following required parameters are here described. - If you wish to input a raw count or normalized table, you can skip the samplesheet parameter as well as the library one and directly input your table using count_table `--count_table your_count_table`. If your count table is normalized, be sure to set the normalization method to none in MAGeCK MLE or MAGeCK RRA using a config file. +If you wish to input a raw count or normalized table, you can skip the samplesheet parameter as well as the library one and directly input your table using count_table `--count_table your_count_table`. If your count table is normalized, be sure to set the normalization method to none in MAGeCK MLE or MAGeCK RRA using a config file. ### Full samplesheet @@ -42,7 +42,6 @@ SRR8983580,SRR8983580.small.fastq.gz,,treatment An [example samplesheet](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/samplesheet_test.csv) has been provided with the pipeline. - ### cutadapt MAGeCK count which is the main alignment software used is normally able to automatically determine the trimming length and sgRNA length, in most cases. Therefore, you don't need to go to this step unless MAGeCK fails to do so by itself. If the nucleotide length in front of sgRNA varies between different reads, you can use cutadapt to remove the adaptor sequences by using the flag `--cutadapt ADAPTER`. @@ -61,7 +60,6 @@ We recommend to run MAGeCK MLE and BAGEL2 as these are the most used and most re CRISPRcleanR is used for gene count normalization and the removal of biases for genomic segments for which copy numbers are amplified. Currently, the pipeline only supports annotation libraries already present in the R package and which can be found [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). - ### Running MAGeCK MLE and BAGEL2 with a contrast file To run both MAGeCK MLE and BAGEL2, you can provide a contrast file with the flag `--contrasts` with the mandatory headers "treatment" and "reference". These two columns should be separated with a dot comma (;) and contain the `csv` extension. You can also integrate several samples/conditions by comma separating them in each column. Please find an example here below : @@ -75,7 +73,7 @@ A full example can be found [here](https://raw.githubusercontent.com/nf-core/tes ### Running MAGeCK RRA only -MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section and also specify `--rra` . +MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section and also specify `--rra` . ### Running MAGeCK MLE only From 14f2545c681be406c8f4fe76de234d77d3fb17cd Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Tue, 16 Jan 2024 13:13:48 +0100 Subject: [PATCH 024/226] Venn diagram first try --- modules/local/bagel2/pr.nf | 4 +- modules/local/matricescreation.nf | 2 +- modules/local/venndiagram.nf | 79 +++++++++++++++++++++++++++++++ workflows/crisprseq_screening.nf | 16 ++++++- 4 files changed, 96 insertions(+), 5 deletions(-) create mode 100644 modules/local/venndiagram.nf diff --git a/modules/local/bagel2/pr.nf b/modules/local/bagel2/pr.nf index e27366d6..12f5e251 100644 --- a/modules/local/bagel2/pr.nf +++ b/modules/local/bagel2/pr.nf @@ -12,7 +12,7 @@ process BAGEL2_PR { tuple val(meta), path(bf), path(reference_essentials), path(reference_nonessentials) output: - tuple val(meta), path("*.pr") , emit: pr + tuple val(meta), path("*.tsv") , emit: pr path "versions.yml" , emit: versions when: @@ -23,7 +23,7 @@ process BAGEL2_PR { def prefix = task.ext.prefix ?: "${meta.id}" """ - BAGEL.py pr -i $bf -o '${meta.treatment}_vs_${meta.reference}.pr' -e $reference_essentials -n $reference_nonessentials $args + BAGEL.py pr -i $bf -o '${meta.treatment}_vs_${meta.reference}.tsv' -e $reference_essentials -n $reference_nonessentials $args cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/local/matricescreation.nf b/modules/local/matricescreation.nf index 43867fa6..24fcbbde 100644 --- a/modules/local/matricescreation.nf +++ b/modules/local/matricescreation.nf @@ -22,7 +22,7 @@ process MATRICESCREATION { #### author: Laurence Kuhlburger #### Released under the MIT license. See git repository (https://github.com/nf-core/crisprseq) for full license text. #### - #### Orient a reference sequence according to reads orientation. + #### data <- read.table("$contrasts", header = TRUE, sep = ";", stringsAsFactors = FALSE) # Loop through each row in the data diff --git a/modules/local/venndiagram.nf b/modules/local/venndiagram.nf new file mode 100644 index 00000000..25f00edc --- /dev/null +++ b/modules/local/venndiagram.nf @@ -0,0 +1,79 @@ +process VENNDIAGRAM { + // tag "$meta.treatment" + label 'process_low' + + + conda "bioconda::r-venndiagram=1.6.16" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/r-venndiagram:1.6.16--r3.3.2_0': + 'biocontainers/r-venndiagram:1.6.16--r3.3.2_0' }" + + input: + tuple val(meta), path(bagel_pr), path(gene_summary) + + output: + tuple val(meta), path("*.txt"), emit: common_list + tuple val(meta), path("*.png"), emit: venn_diagram + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.treatment}" + + """ + #!/usr/bin/env Rscript + + #### author: Laurence Kuhlburger + #### Released under the MIT license. See git repository (https://github.com/nf-core/crisprseq) for full license text. + #### + #### produce a venn diagram + + library(VennDiagram) + mle = read.table('$gene_summary', sep = "\t", + header=TRUE) + bagel = read.table('$bagel_pr', sep = "\t", + header=TRUE) + + filtered_precision_recall <- subset(bagel, FDR > 0.1) + name <- paste0('${meta.id}',".fdr") + filtered_mageck_mle <- subset(mle,name > 0.1) + common_genes <- intersect(filtered_mageck_mle\$Gene, + filtered_precision_recall\$Gene) + + write.table(common_genes, paste0('${meta.id}',"_common_genes_bagel_mle.txt"),sep = "\t", quote = FALSE, row.names=FALSE) + + venn.diagram( + x = list(Set1 = filtered_mageck_mle\$Gene, Set2 = filtered_precision_recall\$Gene), + category.names = c("MAGeCK MLE", "BAGEL2"), + filename = '14_venn_diagramm.png', + output=FALSE + ) + # venn.plot <- venn.diagram(list(Set1 = filtered_mageck_mle\$Gene, Set2 = filtered_precision_recall\$Gene), + # filename= paste0('${meta.id}',"_venn.svg"), category.names = c("MAGeCK MLE", "BAGEL2"), + # # Output features + # imagetype = "svg", + # height = 480, + # width = 480, + # resolution = 300, + # compression = "lzw", + + # # Circles + # lwd = 2, + # lty = 'blank', + # fill = c("#999999", "#E69F00"), + + # # Numbers + # cex = .6, + # fontface = "bold", + # fontfamily = "sans", + # cat.cex = 0.4, + # cat.dist = c(-0.05, -0.02) + #) + + + """ + + +} diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 8eb56572..dc9d8ce9 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -76,6 +76,7 @@ include { BAGEL2_BF } from '../modules/local/bagel2/bf' include { BAGEL2_PR } from '../modules/local/bagel2/pr' include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' include { MATRICESCREATION } from '../modules/local/matricescreation' +include { VENNDIAGRAM } from '../modules/local/venndiagram' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -249,19 +250,30 @@ workflow CRISPRSEQ_SCREENING { ch_mle = ch_counts.combine(ch_design) } if(params.contrasts) { - MATRICESCREATION(params.contrasts) + MATRICESCREATION(Channel.fromPath(params.contrasts)) ch_mle = ch_counts.combine(MATRICESCREATION.out.design_matrix) } ch_mle.map { it -> [[id: it[1].getBaseName()], it[0], it[1]] }.set { ch_designed_mle } + ch_designed_mle.dump(tag: "ch_designed_mle") + //TODO TIDY UP THIS CODE + BAGEL2_PR.out.pr.map { + it -> [[id: it[1].getBaseName()], it[1]] + }.set { ch_testing } + //ch_testing.dump(tag: "BAGEL2 out PR") MAGECK_MLE ( ch_designed_mle ) - ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) + ch_venndiagram = ch_testing.join(MAGECK_MLE.out.gene_summary) + MAGECK_MLE.out.gene_summary.dump(tag: "MLE") + ch_venndiagram.dump(tag: "Venn") + VENNDIAGRAM(ch_venndiagram) + + } From 877617bedc160f6017f40c4370e67c88171324cf Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Thu, 18 Jan 2024 15:46:59 +0000 Subject: [PATCH 025/226] remove docker runOptions for module MAGECK_GRAPHRRA --- conf/modules.config | 6 ++++++ 1 file changed, 6 insertions(+) diff --git a/conf/modules.config b/conf/modules.config index 98a4963f..0c6e4af1 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -299,6 +299,12 @@ process { ] } + withName: 'MAGECK_GRAPHRRA' { + docker { + runOptions = '' + } + } + withName: CUSTOM_DUMPSOFTWAREVERSIONS { publishDir = [ path: { "${params.outdir}/pipeline_info" }, From 9248ab3026cf794076639fc460e0fc8c6b2af4c8 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 18 Jan 2024 15:48:32 +0000 Subject: [PATCH 026/226] [automated] Fix linting with Prettier --- .devcontainer/devcontainer.json | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe3..4a9bc5c7 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,11 +18,11 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.linting.pylintPath": "/opt/conda/bin/pylint", }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] - } - } + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], + }, + }, } From 51f8248ddd874142c678b044d4ab3b0966e4d51b Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Fri, 19 Jan 2024 13:05:34 +0100 Subject: [PATCH 027/226] Fix magecl mle process and matrix creation --- modules/local/matricescreation.nf | 46 ++++++++++++++---------------- modules/nf-core/mageck/mle/main.nf | 2 +- workflows/crisprseq_screening.nf | 30 ++++++++++++------- 3 files changed, 41 insertions(+), 37 deletions(-) diff --git a/modules/local/matricescreation.nf b/modules/local/matricescreation.nf index 43867fa6..9a6d8b56 100644 --- a/modules/local/matricescreation.nf +++ b/modules/local/matricescreation.nf @@ -7,10 +7,10 @@ process MATRICESCREATION { 'biocontainers/mulled-v2-6de07928379e6eface08a0019c4a1d6b5192e805:0d77388f37ddd923a087f7792e30e83ab54c918c-0' }" input: - path(contrasts) + val(meta) output: - path("*.txt"), emit: design_matrix + tuple val(meta), path("*.txt"), emit: design_matrix when: task.ext.when == null || task.ext.when @@ -22,30 +22,26 @@ process MATRICESCREATION { #### author: Laurence Kuhlburger #### Released under the MIT license. See git repository (https://github.com/nf-core/crisprseq) for full license text. #### - #### Orient a reference sequence according to reads orientation. + #### - data <- read.table("$contrasts", header = TRUE, sep = ";", stringsAsFactors = FALSE) # Loop through each row in the data - for (i in 1:nrow(data)) { - # Extract control and treatment samples for the current row - control_samples <- unlist(strsplit(data\$reference[i], ",")) - treatment_samples <- unlist(strsplit(data\$treatment[i], ",")) - - # Create a vector of all unique samples - all_samples <- unique(c(control_samples, treatment_samples)) - - # Initialize a matrix to store the design matrix - design_matrix <- data.frame(matrix(0, nrow = length(all_samples), ncol = 3, - dimnames = list(all_samples, c("Samples", "baseline", paste0(gsub(',', '_', data\$treatment[i] ),"_vs_", data\$reference[i]))))) - - # Set baseline and treatment values in the design matrix - design_matrix[, "Samples"] <- rownames(design_matrix) - design_matrix\$baseline <- 1 - design_matrix[treatment_samples, paste0(gsub(',', '_', data\$treatment[1] ),"_vs_",gsub(",","_",data\$reference[i]))] <- 1 - - # Print the design matrix to a file - output_file <- paste0(gsub(',', '_', data\$treatment[1] ),"_vs_",gsub(",","_",data\$reference[i]),".txt") - write.table(design_matrix, output_file, sep = "\t", quote = FALSE, row.names=FALSE) - } + control_samples <- unlist(strsplit('${meta.reference}', ",")) + treatment_samples <- unlist(strsplit('$meta.treatment', ",")) + all_samples <- unique(c(control_samples, treatment_samples)) + design_matrix <- data.frame(matrix(0, nrow = length(all_samples), ncol = 3, + dimnames = list(all_samples, + c("Samples", "baseline", + paste0(gsub(',', '_', '$meta.treatment'),"_vs_",gsub(',','_','$meta.reference')))))) + name = paste0(gsub(',', '_', '$meta.treatment' ),"_vs_", gsub(',', '_','$meta.reference')) + # Set baseline and treatment values in the design matrix + design_matrix[, "Samples"] <- rownames(design_matrix) + design_matrix\$baseline <- 1 + design_matrix[treatment_samples, name] <- 1 + design_matrix[treatment_samples, paste0(gsub(',', '_', '$meta.treatment'),"_vs_",gsub(",","_",'$meta.reference'))] <- 1 + + # Print the design matrix to a file + output_file <- paste0(gsub(',', '_', '$meta.treatment' ),"_vs_",gsub(",","_",'$meta.reference'),".txt") + write.table(design_matrix, output_file, sep = "\t", quote = FALSE, row.names=FALSE) + """ } diff --git a/modules/nf-core/mageck/mle/main.nf b/modules/nf-core/mageck/mle/main.nf index 75ff06f2..dedee3cf 100644 --- a/modules/nf-core/mageck/mle/main.nf +++ b/modules/nf-core/mageck/mle/main.nf @@ -8,7 +8,7 @@ process MAGECK_MLE { 'biocontainers/mageck:0.5.9--py37h6bb024c_0' }" input: - tuple val(meta), path(count_table), path(design_matrix) + tuple val(meta), path(design_matrix), path(count_table) output: tuple val(meta), path("*.gene_summary.txt") , emit: gene_summary diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 8eb56572..4408c005 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -206,8 +206,8 @@ workflow CRISPRSEQ_SCREENING { if(params.contrasts) { Channel.fromPath(params.contrasts) .splitCsv(header:true, sep:';' ) - .set { ch_bagel } - counts = ch_bagel.combine(ch_counts) + .set { ch_contrasts } + counts = ch_contrasts.combine(ch_counts) //Define non essential and essential genes channels for bagel2 ch_bagel_reference_essentials= Channel.value(params.bagel_reference_essentials) @@ -249,18 +249,26 @@ workflow CRISPRSEQ_SCREENING { ch_mle = ch_counts.combine(ch_design) } if(params.contrasts) { - MATRICESCREATION(params.contrasts) - ch_mle = ch_counts.combine(MATRICESCREATION.out.design_matrix) + MATRICESCREATION(ch_contrasts) + ch_mle = MATRICESCREATION.out.design_matrix.combine(ch_counts) + MAGECK_MLE (ch_mle) + ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) } - ch_mle.map { - it -> [[id: it[1].getBaseName()], it[0], it[1]] - }.set { ch_designed_mle } - MAGECK_MLE ( - ch_designed_mle - ) - ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) + // ch_test.map { + // it -> [[id: it[0].getBaseName()], it] + //}.set { ch_designed_test } + //ch_designed_test.dump(tag: "ch_test design") + // ch_mle.map { + // it -> [[id: it[1].getBaseName()], it[0], it[1]] + //}.set { ch_designed_mle } + + // MAGECK_MLE ( + // ch_designed_mle + //) + + // } From cace7ab7c32c6b32772867db976a54c95e2458e1 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Fri, 19 Jan 2024 12:24:17 +0000 Subject: [PATCH 028/226] add seed to bagel2_bf to avoid random errors due to a too small sample --- conf/test_screening.config | 6 ++++++ conf/test_screening_full.config | 6 ++++++ conf/test_screening_paired.config | 6 ++++++ conf/test_screening_rra.config | 6 ++++++ 4 files changed, 24 insertions(+) diff --git a/conf/test_screening.config b/conf/test_screening.config index 0b5c5b70..8a6cfc9e 100644 --- a/conf/test_screening.config +++ b/conf/test_screening.config @@ -26,3 +26,9 @@ params { library = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/brunello_target_sequence.txt" contrasts = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/rra_contrasts.txt" } + +process { + withName: BAGEL2_BF { + ext.args = '-s 3' // Seed to avoid random errors due to a too small sample + } +} diff --git a/conf/test_screening_full.config b/conf/test_screening_full.config index 57c0f066..21709de9 100644 --- a/conf/test_screening_full.config +++ b/conf/test_screening_full.config @@ -21,3 +21,9 @@ params { mle_design_matrix = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/full_test/drugA_drugB_vs_treatment.txt" library = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/brunello_target_sequence.txt" } + +process { + withName: BAGEL2_BF { + ext.args = '-s 3' // Seed to avoid random errors due to a too small sample + } +} diff --git a/conf/test_screening_paired.config b/conf/test_screening_paired.config index d8874eff..90867efd 100644 --- a/conf/test_screening_paired.config +++ b/conf/test_screening_paired.config @@ -24,3 +24,9 @@ params { analysis = 'screening' library = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/brunello_target_sequence.txt" } + +process { + withName: BAGEL2_BF { + ext.args = '-s 3' // Seed to avoid random errors due to a too small sample + } +} diff --git a/conf/test_screening_rra.config b/conf/test_screening_rra.config index 5042ec35..bdda3a07 100644 --- a/conf/test_screening_rra.config +++ b/conf/test_screening_rra.config @@ -27,3 +27,9 @@ params { contrasts = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/rra_contrasts.txt" rra = true } + +process { + withName: BAGEL2_BF { + ext.args = '-s 3' // Seed to avoid random errors due to a too small sample + } +} From b3f7f05db3a79f7a0ac4ca3650ff5e419834f448 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Fri, 19 Jan 2024 15:21:10 +0000 Subject: [PATCH 029/226] replace docker.runOptions by process.containerOptions --- conf/modules.config | 10 ++++------ nextflow.config | 18 +++++++++--------- 2 files changed, 13 insertions(+), 15 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 0c6e4af1..0c057c08 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -122,6 +122,10 @@ process { ] } + withName: MAGECK_GRAPHRRA { + containerOptions = '' + } + withName: CRISPRCLEANR_NORMALIZE { publishDir = [ path: { "${params.outdir}/crisprcleanr/normalization/" }, @@ -299,12 +303,6 @@ process { ] } - withName: 'MAGECK_GRAPHRRA' { - docker { - runOptions = '' - } - } - withName: CUSTOM_DUMPSOFTWAREVERSIONS { publishDir = [ path: { "${params.outdir}/pipeline_info" }, diff --git a/nextflow.config b/nextflow.config index f4722221..cf47750c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -131,17 +131,17 @@ profiles { apptainer.enabled = false } docker { - docker.enabled = true - conda.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false - docker.runOptions = '-u $(id -u):$(id -g)' + docker.enabled = true + conda.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false + process.containerOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + process.containerOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { singularity.enabled = true From 33fd577c3ab954f6d5c18110304051a94da70226 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 22 Jan 2024 12:35:12 +0100 Subject: [PATCH 030/226] Fix matrices creation --- workflows/crisprseq_screening.nf | 10 ++++++---- 1 file changed, 6 insertions(+), 4 deletions(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 4408c005..aa9770e6 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -245,17 +245,19 @@ workflow CRISPRSEQ_SCREENING { } if((params.mle_design_matrix) || (params.contrasts && !params.rra)) { + //TODO FINISH THIS PART!! If you see this in a PR please point it out to me if(params.mle_design_matrix) { - ch_mle = ch_counts.combine(ch_design) - } + ch_mle = ch_design.combine.ch_counts + ch_design.map { + it -> [[id: it.getBaseName()], it] + }.set { ch_designed_test } + } if(params.contrasts) { MATRICESCREATION(ch_contrasts) ch_mle = MATRICESCREATION.out.design_matrix.combine(ch_counts) MAGECK_MLE (ch_mle) ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) } - - // ch_test.map { // it -> [[id: it[0].getBaseName()], it] //}.set { ch_designed_test } From 0b04c09ae4f4f6443179413a6934eb3a7df0eb2c Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Tue, 23 Jan 2024 14:12:02 +0100 Subject: [PATCH 031/226] Trying to fix crisprcleanr --- .../nf-core/crisprcleanr/normalize/main.nf | 35 ++----------------- workflows/crisprseq_screening.nf | 3 +- 2 files changed, 5 insertions(+), 33 deletions(-) diff --git a/modules/nf-core/crisprcleanr/normalize/main.nf b/modules/nf-core/crisprcleanr/normalize/main.nf index 33e4ecd1..69d12808 100644 --- a/modules/nf-core/crisprcleanr/normalize/main.nf +++ b/modules/nf-core/crisprcleanr/normalize/main.nf @@ -8,7 +8,7 @@ process CRISPRCLEANR_NORMALIZE { 'biocontainers/r-crisprcleanr:3.0.0--r42hdfd78af_1' }" input: - tuple val(meta), path(count_file), val(library_file) + tuple val(meta), path(count_file), val(library_value), path(library_file) val(min_reads) val(min_targeted_genes) @@ -28,38 +28,9 @@ process CRISPRCLEANR_NORMALIZE { #!/usr/bin/env Rscript library(CRISPRcleanR) library(dplyr) - data('${library_file}') - count_file <- read.delim('${count_file}',header=T,sep = "\t") - count_file_to_normalize <- count_file %>% dplyr::left_join(get('${library_file}'), by=c("sgRNA"="Target.Context.Sequence"),multiple = "all") + + - count_file_to_normalize <- count_file_to_normalize %>% - dplyr::select(colnames(count_file),CODE,-sgRNA) - - names(count_file_to_normalize)[names(count_file_to_normalize) == 'Gene'] <- 'gene' - names(count_file_to_normalize)[names(count_file_to_normalize) == 'CODE'] <- 'sgRNA' - count_file_to_normalize <- count_file_to_normalize %>% dplyr::select(sgRNA, gene, everything()) - - #crisprcleanr function - normANDfcs <- ccr.NormfoldChanges(Dframe=count_file_to_normalize,saveToFig = FALSE,min_reads=${min_reads},EXPname="${prefix}", libraryAnnotation=get('${library_file}'),display=FALSE) - gwSortedFCs <- ccr.logFCs2chromPos(normANDfcs[["logFCs"]],get('${library_file}')) - correctedFCs <- ccr.GWclean(gwSortedFCs,display=FALSE,label='${meta}') - correctedCounts <- ccr.correctCounts('${meta}', - normANDfcs[["norm_counts"]], - correctedFCs, - get('${library_file}'), - minTargetedGenes=${min_targeted_genes}, - OutDir='./') - - write.table(correctedCounts, file=paste0("${prefix}","_norm_table.tsv"),row.names=FALSE,quote=FALSE,sep="\t") - - #version - version_file_path <- "versions.yml" - version_crisprcleanr <- paste(unlist(packageVersion("CRISPRcleanR")), collapse = ".") - f <- file(version_file_path, "w") - writeLines('"${task.process}":', f) - writeLines(" crisprcleanr: ", f, sep = "") - writeLines(version_crisprcleanr, f) - close(f) """ } diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 4cf51906..215e2adc 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -21,7 +21,7 @@ if (params.crisprcleanr) { if(params.crisprcleanr.endsWith(".csv")) { ch_crisprcleanr_file = file(params.crisprcleanr) } else { - ch_crisprcleanr_value = Channel.value(params.crisprcleanr) + ch_crisprcleanr_value = Channel.value(params.crisprcleanr) } } @@ -180,6 +180,7 @@ workflow CRISPRSEQ_SCREENING { if(params.crisprcleanr.endsWith(".csv")) { CRISPRCLEANR_NORMALIZE( ch_crispr_normalize.collect(), + Channel.empty(), ch_crisprcleanr_file, params.min_reads, params.min_targeted_genes From d815aea314516df01be712054ee8449bb7d3e24a Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Thu, 25 Jan 2024 08:47:49 +0100 Subject: [PATCH 032/226] Fix crisprcleanr --- docs/usage/screening.md | 13 ++++- .../nf-core/crisprcleanr/normalize/main.nf | 53 +++++++++++++++++-- modules/nf-core/mageck/mle/main.nf | 4 +- workflows/crisprseq_screening.nf | 8 +-- 4 files changed, 67 insertions(+), 11 deletions(-) diff --git a/docs/usage/screening.md b/docs/usage/screening.md index b0c6dcac..171b4781 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -21,7 +21,7 @@ nextflow run nf-core/crisprseq --analysis screening --input samplesheet.csv --li ``` The following required parameters are here described. -If you wish to input a raw count or normalized table, you can skip the samplesheet parameter as well as the library one and directly input your table using count_table `--count_table your_count_table`. If your count table is normalized, be sure to set the normalization method to none in MAGeCK MLE or MAGeCK RRA using a config file. +If you wish to input a raw count or normalized table, you can skip the samplesheet parameter as well as the library one and directly input your table using count_table `--count_table your_count_table`. Your count table should contain the following columns : sgRNA and gene. If your count table is normalized, be sure to set the normalization method to none in MAGeCK MLE or MAGeCK RRA using a config file. ### Full samplesheet @@ -58,7 +58,16 @@ We recommend to run MAGeCK MLE and BAGEL2 as these are the most used and most re ### Running CRISPRcleanR -CRISPRcleanR is used for gene count normalization and the removal of biases for genomic segments for which copy numbers are amplified. Currently, the pipeline only supports annotation libraries already present in the R package and which can be found [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). +CRISPRcleanR is used for gene count normalization and the removal of biases for genomic segments for which copy numbers are amplified. Currently, the pipeline supports annotation libraries already present in the R package or a annotation file the user can provide. +Most used library already have an annotation dataset which you can find [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). +Otherwise, if you wish to provide your own file, please provide it in csv form, and make sure it follows the following format : + +,CODE,GENES,EXONE,CHRM,STRAND,STARTpos,ENDpos +AAAAAAAAAAAATGCATTCT,NM_183035.1,Defb34,ex2,8,-,19126349,19126369 +AAAAAAAAATAAGCTCACCC,NM_001170853.1,Mndal,ex5,1,+,173872968,173872988 +AAAAAAAATCCTGTCGCCCA,NM_001039049.1,Cox8c,ex1,12,+,102899487,102899507 +AAAAAAATCGGCATACCATG,NM_178627.3,Poldip3,ex4,15,-,83135295,83135315 +AAAAAAATGACATTACTGCA,NM_026602.3,Bcas2,ex4,3,+,103174386,103174406 ### Running MAGeCK MLE and BAGEL2 with a contrast file diff --git a/modules/nf-core/crisprcleanr/normalize/main.nf b/modules/nf-core/crisprcleanr/normalize/main.nf index 69d12808..d8969379 100644 --- a/modules/nf-core/crisprcleanr/normalize/main.nf +++ b/modules/nf-core/crisprcleanr/normalize/main.nf @@ -8,7 +8,9 @@ process CRISPRCLEANR_NORMALIZE { 'biocontainers/r-crisprcleanr:3.0.0--r42hdfd78af_1' }" input: - tuple val(meta), path(count_file), val(library_value), path(library_file) + tuple val(meta), path(count_file) + val(library_value) + path(library_file) val(min_reads) val(min_targeted_genes) @@ -28,9 +30,54 @@ process CRISPRCLEANR_NORMALIZE { #!/usr/bin/env Rscript library(CRISPRcleanR) library(dplyr) - - + print('${library_value}') + count_file <- read.delim('${count_file}',header=T,sep = "\t") + count_file <- count_file[!duplicated(count_file\$sgRNA), ] + if('${library_file}' == "") { + data('${library_value}') + library <- as.data.frame(get('${library_value}')) + #colnames(library) + #print(head(count_file)) + #print(head(library)) + count_file_to_normalize <- count_file %>% dplyr::left_join(library, by=c("sgRNA"="seq"),multiple = "all") + count_file_to_normalize <- count_file_to_normalize %>% + dplyr::select(colnames(count_file),CODE,-sgRNA) + + names(count_file_to_normalize)[names(count_file_to_normalize) == 'Gene'] <- 'gene' + names(count_file_to_normalize)[names(count_file_to_normalize) == 'CODE'] <- 'sgRNA' + count_file_to_normalize <- count_file_to_normalize %>% dplyr::select(sgRNA, gene, everything()) + } else { + try(library <- read.delim('${library_file}',header=T,sep = ",")) + duplicates <- duplicated(library[, 1]) + unique_rows <- !duplicates + library <- library[unique_rows, , drop = FALSE] + rownames(library) = library[,1] + library = library[order(rownames(library)),] + library = library[,-1] + count_file_to_normalize <- count_file + } + + normANDfcs <- ccr.NormfoldChanges(Dframe=count_file_to_normalize,saveToFig = FALSE,min_reads=${min_reads},EXPname="${prefix}", libraryAnnotation=library,display=FALSE) + gwSortedFCs <- ccr.logFCs2chromPos(normANDfcs[["logFCs"]],library) + correctedFCs <- ccr.GWclean(gwSortedFCs,display=FALSE,label='crisprcleanr') + correctedCounts <- ccr.correctCounts('crisprcleanr', + normANDfcs[["norm_counts"]], + correctedFCs, + library, + minTargetedGenes=${min_targeted_genes}, + OutDir='./') + + write.table(correctedCounts, file=paste0("crisprcleanr","_norm_table.tsv"),row.names=FALSE,quote=FALSE,sep="\t") + + #version + version_file_path <- "versions.yml" + version_crisprcleanr <- paste(unlist(packageVersion("CRISPRcleanR")), collapse = ".") + f <- file(version_file_path, "w") + writeLines('"${task.process}":', f) + writeLines(" crisprcleanr: ", f, sep = "") + writeLines(version_crisprcleanr, f) + close(f) """ } diff --git a/modules/nf-core/mageck/mle/main.nf b/modules/nf-core/mageck/mle/main.nf index dedee3cf..462274ae 100644 --- a/modules/nf-core/mageck/mle/main.nf +++ b/modules/nf-core/mageck/mle/main.nf @@ -1,5 +1,5 @@ process MAGECK_MLE { - tag "$meta.id" + tag "${meta.treatment}_vs_${meta.reference}" label 'process_high' conda "${moduleDir}/environment.yml" @@ -29,7 +29,7 @@ process MAGECK_MLE { --threads $task.cpus \\ -k $count_table \\ -d $design_matrix \\ - -n $prefix + -n "${meta.treatment}_vs_${meta.reference}" cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index b8ba275d..6855d6ac 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -180,7 +180,7 @@ workflow CRISPRSEQ_SCREENING { if(params.crisprcleanr.endsWith(".csv")) { CRISPRCLEANR_NORMALIZE( ch_crispr_normalize.collect(), - Channel.empty(), + '', ch_crisprcleanr_file, params.min_reads, params.min_targeted_genes @@ -190,7 +190,7 @@ workflow CRISPRSEQ_SCREENING { CRISPRCLEANR_NORMALIZE( ch_crispr_normalize.collect(), ch_crisprcleanr_value, - Channel.empty(), + [], params.min_reads, params.min_targeted_genes) } @@ -228,8 +228,8 @@ workflow CRISPRSEQ_SCREENING { counts = ch_contrasts.combine(ch_counts) //Define non essential and essential genes channels for bagel2 - ch_bagel_reference_essentials= Channel.value(params.bagel_reference_essentials) - ch_bagel_reference_nonessentials= Channel.value(params.bagel_reference_nonessentials) + ch_bagel_reference_essentials= Channel.fromPath(params.bagel_reference_essentials) + ch_bagel_reference_nonessentials= Channel.fromPath(params.bagel_reference_nonessentials) BAGEL2_FC ( counts From 5ea4d21b93d94b30305200227e4f6a27431e23f2 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Fri, 26 Jan 2024 14:14:29 +0100 Subject: [PATCH 033/226] fix bagel tentative --- conf/modules.config | 2 +- modules/local/bagel2/bf.nf | 4 ++-- workflows/crisprseq_screening.nf | 4 ++-- 3 files changed, 5 insertions(+), 5 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 0c057c08..2c6330b3 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -104,7 +104,7 @@ process { withName: MAGECK_MLE { publishDir = [ - path: { "${params.outdir}/mageck/mle/${meta.id}/" }, + path: { "${params.outdir}/mageck/mle/${meta.treatment}_vs_${meta.reference}/" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] diff --git a/modules/local/bagel2/bf.nf b/modules/local/bagel2/bf.nf index 3ff86603..91ab780c 100644 --- a/modules/local/bagel2/bf.nf +++ b/modules/local/bagel2/bf.nf @@ -11,8 +11,8 @@ process BAGEL2_BF { input: tuple val(meta), path(foldchange) - path(reference_essentials) - path(reference_nonessentials) + val(reference_essentials) + val(reference_nonessentials) output: tuple val(meta), path("*.bf"), emit: bf diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 6855d6ac..7095d96c 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -228,8 +228,8 @@ workflow CRISPRSEQ_SCREENING { counts = ch_contrasts.combine(ch_counts) //Define non essential and essential genes channels for bagel2 - ch_bagel_reference_essentials= Channel.fromPath(params.bagel_reference_essentials) - ch_bagel_reference_nonessentials= Channel.fromPath(params.bagel_reference_nonessentials) + ch_bagel_reference_essentials= Channel.from(params.bagel_reference_essentials).first() + ch_bagel_reference_nonessentials= Channel.from(params.bagel_reference_nonessentials).first() BAGEL2_FC ( counts From 450541aaedd3b401711e5cff264aeed930c58f3b Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 29 Jan 2024 15:19:42 +0100 Subject: [PATCH 034/226] add nf --- workflows/crisprseq_screening.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 7095d96c..797e5443 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -228,8 +228,8 @@ workflow CRISPRSEQ_SCREENING { counts = ch_contrasts.combine(ch_counts) //Define non essential and essential genes channels for bagel2 - ch_bagel_reference_essentials= Channel.from(params.bagel_reference_essentials).first() - ch_bagel_reference_nonessentials= Channel.from(params.bagel_reference_nonessentials).first() + ch_bagel_reference_essentials= Channel.value(params.bagel_reference_essentials) + ch_bagel_reference_nonessentials= Channel.value(params.bagel_reference_nonessentials) BAGEL2_FC ( counts From f27bf8ac64b83b71d6f034df23c02741989e748c Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 29 Jan 2024 15:30:50 +0100 Subject: [PATCH 035/226] fix markdown table? --- docs/usage/screening.md | 13 +++++++------ 1 file changed, 7 insertions(+), 6 deletions(-) diff --git a/docs/usage/screening.md b/docs/usage/screening.md index 171b4781..b4b7c11b 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -62,12 +62,13 @@ CRISPRcleanR is used for gene count normalization and the removal of biases for Most used library already have an annotation dataset which you can find [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). Otherwise, if you wish to provide your own file, please provide it in csv form, and make sure it follows the following format : -,CODE,GENES,EXONE,CHRM,STRAND,STARTpos,ENDpos -AAAAAAAAAAAATGCATTCT,NM_183035.1,Defb34,ex2,8,-,19126349,19126369 -AAAAAAAAATAAGCTCACCC,NM_001170853.1,Mndal,ex5,1,+,173872968,173872988 -AAAAAAAATCCTGTCGCCCA,NM_001039049.1,Cox8c,ex1,12,+,102899487,102899507 -AAAAAAATCGGCATACCATG,NM_178627.3,Poldip3,ex4,15,-,83135295,83135315 -AAAAAAATGACATTACTGCA,NM_026602.3,Bcas2,ex4,3,+,103174386,103174406 +| ,CODE | GENES | EXONE | CHRM | STRAND | STARTpos | ENDpos | +|----------------------|-------------------|-------|------|--------|-----------|-----------| +| AAAAAAAAAAAATGCATTCT | NM_183035.1 | Defb34| ex2 | 8 | - | 19126349 | 19126369 | +| AAAAAAAAATAAGCTCACCC | NM_001170853.1 | Mndal | ex5 | 1 | + | 173872968 | 173872988 | +| AAAAAAAATCCTGTCGCCCA | NM_001039049.1 | Cox8c | ex1 | 12 | + | 102899487 | 102899507 | +| AAAAAAATCGGCATACCATG | NM_178627.3 | Poldip3| ex4 | 15 | - | 83135295 | 83135315 | +| AAAAAAATGACATTACTGCA | NM_026602.3 | Bcas2 | ex4 | 3 | + | 103174386 | 103174406 | ### Running MAGeCK MLE and BAGEL2 with a contrast file From 21da1446414037efdba5fb7752cafb1b0db824b7 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 29 Jan 2024 14:50:24 +0000 Subject: [PATCH 036/226] Template update for nf-core/tools version 2.12 --- .editorconfig | 8 + .github/workflows/awsfulltest.yml | 2 +- .github/workflows/awstest.yml | 2 +- .github/workflows/branch.yml | 2 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 67 +++++ .github/workflows/fix-linting.yml | 72 +++-- .github/workflows/linting.yml | 61 +--- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 4 +- .gitpod.yml | 1 + .pre-commit-config.yaml | 7 +- CHANGELOG.md | 2 +- README.md | 8 +- assets/email_template.html | 2 +- assets/email_template.txt | 2 +- assets/multiqc_config.yml | 6 +- assets/nf-core-crisprseq_logo_light.png | Bin 74216 -> 77402 bytes docs/images/nf-core-crisprseq_logo_dark.png | Bin 74344 -> 29473 bytes docs/images/nf-core-crisprseq_logo_light.png | Bin 74216 -> 25158 bytes lib/WorkflowMain.groovy | 16 +- lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes main.nf | 2 +- modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 2 +- .../custom/dumpsoftwareversions/main.nf | 4 +- .../dumpsoftwareversions/tests/main.nf.test | 7 +- .../tests/main.nf.test.snap | 50 ++-- modules/nf-core/fastqc/tests/main.nf.test | 265 ++++++++++++------ .../nf-core/fastqc/tests/main.nf.test.snap | 12 +- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 6 +- modules/nf-core/multiqc/meta.yml | 1 - modules/nf-core/multiqc/tests/main.nf.test | 48 +++- .../nf-core/multiqc/tests/main.nf.test.snap | 21 ++ nextflow.config | 5 +- pyproject.toml | 17 +- workflows/crisprseq.nf | 7 + 38 files changed, 494 insertions(+), 227 deletions(-) create mode 100644 .github/workflows/download_pipeline.yml delete mode 100644 lib/nfcore_external_java_deps.jar create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap diff --git a/.editorconfig b/.editorconfig index b6b31907..9b990088 100644 --- a/.editorconfig +++ b/.editorconfig @@ -22,3 +22,11 @@ indent_size = unset [/assets/email*] indent_size = unset + +# ignore Readme +[README.md] +indent_style = unset + +# ignore python +[*.{py}] +indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 92ebecb0..b393817c 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 0d650d8e..f46f773a 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 4880de34..29192d3d 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v1 + uses: mshick/add-pr-comment@v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index 694e90ec..e37cfda5 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v7 + - uses: actions/stale@v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml new file mode 100644 index 00000000..8611458a --- /dev/null +++ b/.github/workflows/download_pipeline.yml @@ -0,0 +1,67 @@ +name: Test successful pipeline download with 'nf-core download' + +# Run the workflow when: +# - dispatched manually +# - when a PR is opened or reopened to master branch +# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. +on: + workflow_dispatch: + pull_request: + types: + - opened + branches: + - master + pull_request_target: + branches: + - master + +env: + NXF_ANSI_LOG: false + +jobs: + download: + runs-on: ubuntu-latest + steps: + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + + - uses: actions/setup-python@v5 + with: + python-version: "3.11" + architecture: "x64" + - uses: eWaterCycle/setup-singularity@v7 + with: + singularity-version: 3.8.3 + + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install git+https://github.com/nf-core/tools.git@dev + + - name: Get the repository name and current branch set as environment variable + run: | + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + + - name: Download the pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + run: | + nf-core download ${{ env.REPO_LOWERCASE }} \ + --revision ${{ env.REPO_BRANCH }} \ + --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ + --compress "none" \ + --container-system 'singularity' \ + --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-cache-utilisation 'amend' \ + --download-configuration + + - name: Inspect download + run: tree ./${{ env.REPOTITLE_LOWERCASE }} + + - name: Run the downloaded pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 2007e653..9fc36a6c 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -4,7 +4,7 @@ on: types: [created] jobs: - deploy: + fix-linting: # Only run if comment is on a PR with the main repo, and if it contains the magic keywords if: > contains(github.event.comment.html_url, '/pull/') && @@ -13,10 +13,17 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} + # indication that the linting is being fixed + - name: React on comment + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: eyes + # Action runs on the issue comment, so we don't get the PR by default # Use the gh cli to check out the PR - name: Checkout Pull Request @@ -24,32 +31,59 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v4 + # Install and run pre-commit + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + with: + python-version: 3.11 - - name: Install Prettier - run: npm install -g prettier @prettier/plugin-php + - name: Install pre-commit + run: pip install pre-commit - # Check that we actually need to fix something - - name: Run 'prettier --check' - id: prettier_status - run: | - if prettier --check ${GITHUB_WORKSPACE}; then - echo "result=pass" >> $GITHUB_OUTPUT - else - echo "result=fail" >> $GITHUB_OUTPUT - fi + - name: Run pre-commit + id: pre-commit + run: pre-commit run --all-files + continue-on-error: true - - name: Run 'prettier --write' - if: steps.prettier_status.outputs.result == 'fail' - run: prettier --write ${GITHUB_WORKSPACE} + # indication that the linting has finished + - name: react if linting finished succesfully + if: steps.pre-commit.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: "+1" - name: Commit & push changes - if: steps.prettier_status.outputs.result == 'fail' + id: commit-and-push + if: steps.pre-commit.outcome == 'failure' run: | git config user.email "core@nf-co.re" git config user.name "nf-core-bot" git config push.default upstream git add . git status - git commit -m "[automated] Fix linting with Prettier" + git commit -m "[automated] Fix code linting" git push + + - name: react if linting errors were fixed + id: react-if-fixed + if: steps.commit-and-push.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: hooray + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: confused + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + issue-number: ${{ github.event.issue.number }} + body: | + @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually. + See [CI log](https://github.com/nf-core/crisprseq/actions/runs/${{ github.run_id }}) for more details. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 905c58e4..81cd098e 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,61 +11,22 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - uses: actions/checkout@v4 - - uses: actions/setup-node@v4 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - uses: actions/setup-node@v4 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.11 + uses: actions/setup-python@v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: 3.11 + cache: "pip" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest @@ -76,7 +37,7 @@ jobs: - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.11" architecture: "x64" @@ -99,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 0bbcd30f..147bcd10 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v2 + uses: dawidd6/action-download-artifact@v3 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 6ad33927..21ac3f06 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -24,7 +24,7 @@ jobs: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +56,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.0.2 + - uses: zentered/bluesky-post-action@v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/.gitpod.yml b/.gitpod.yml index acf72695..363d5b1d 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -7,6 +7,7 @@ tasks: - name: unset JAVA_TOOL_OPTIONS command: | unset JAVA_TOOL_OPTIONS + vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 0c31cdb9..af57081f 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -1,5 +1,10 @@ repos: - repo: https://github.com/pre-commit/mirrors-prettier - rev: "v2.7.1" + rev: "v3.1.0" hooks: - id: prettier + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python + rev: "2.7.3" + hooks: + - id: editorconfig-checker + alias: ec diff --git a/CHANGELOG.md b/CHANGELOG.md index 4bfca101..3b744cbd 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.1.1 - [date] +## v2.2.0dev - [date] Initial release of nf-core/crisprseq, created with the [nf-core](https://nf-co.re/) template. diff --git a/README.md b/README.md index 4e25b29c..177d1c85 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,9 @@ -# ![nf-core/crisprseq](docs/images/nf-core-crisprseq_logo_light.png#gh-light-mode-only) ![nf-core/crisprseq](docs/images/nf-core-crisprseq_logo_dark.png#gh-dark-mode-only) - +

+ + + nf-core/crisprseq + +

[![GitHub Actions CI Status](https://github.com/nf-core/crisprseq/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/crisprseq/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/crisprseq/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/crisprseq/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/crisprseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) diff --git a/assets/email_template.html b/assets/email_template.html index ec162f42..07ce59a6 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -12,7 +12,7 @@ -

nf-core/crisprseq v${version}

+

nf-core/crisprseq ${version}

Run Name: $runName

<% if (!success){ diff --git a/assets/email_template.txt b/assets/email_template.txt index da4d1c29..0061a387 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -4,7 +4,7 @@ |\\ | |__ __ / ` / \\ |__) |__ } { | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, `._,._,' - nf-core/crisprseq v${version} + nf-core/crisprseq ${version} ---------------------------------------------------- Run Name: $runName diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index e7d12291..1e43bf26 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,9 +1,7 @@ report_comment: > - - This report has been generated by the nf-core/crisprseq + This report has been generated by the nf-core/crisprseq analysis pipeline. 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-24,7 +24,7 @@ class WorkflowMain { // // Validate parameters and print summary to screen // - public static void initialise(workflow, params, log) { + public static void initialise(workflow, params, log, args) { // Print workflow version and exit on --version if (params.version) { @@ -35,6 +35,8 @@ class WorkflowMain { // Check that a -profile or Nextflow config has been provided to run the pipeline NfcoreTemplate.checkConfigProvided(workflow, log) + // Check that the profile doesn't contain spaces and doesn't end with a trailing comma + checkProfile(workflow.profile, args, log) // Check that conda channels are set-up correctly if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { @@ -60,4 +62,16 @@ class WorkflowMain { } return null } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } } diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar deleted file mode 100644 index 805c8bb5e4fd43a12a5891eea5a68788309629b0..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX zni_mKLGakt&P12{r^KCD0GPa9sIuy`fu+agt?8O-hY9i{WloGe}y@kSsDEY_Fud9 z-?2ab4J)MQXe4E3Xl-U<>PRMQZ)0m@@90KnV5#Te5Tz`pvZ0Lj4F<9%Flh(`Qb;Tn zMV!iFmH{S^fF*j#MCKRUd`7m>9||*;WE32I_6pg4-Q1C#ke$$aVmP-TtfJ+j+N9;Q z>bxN=bCVQkg$Jzty71(+~A9}ZfANuYIVnTdW!??Sj)Ti-Qpk8XgPDab8pk2QD&dwUl=iFhd1c$C2uMVagr zWc@z9s$#g__{3?VGb#)^fSNp^lZh zU>MI!D?+L)nyth{@@L0+B&_M8iR08L)G6#PZQLFmvz-9DlZkwRWigRIyI4xYr7>tRJ-N6MlBB`nJQ!!8D)mI2lB3PP;dC{<{Td>^ 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b/modules.json index b220a458..c91b896f 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", + "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "4ab13872435962dadc239979554d13709e20bf29", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index f0c63f69..9b3272bc 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.17 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 7685b33c..f2187611 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test index eec1db10..b1e1630b 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -31,7 +31,12 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } ) } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap index 4274ed57..5f59a936 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -1,27 +1,33 @@ { "Should run without failures": { "content": [ - { - "0": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ], - "1": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "2": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "mqc_yml": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "versions": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "yml": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ] - } + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] ], - "timestamp": "2023-11-03T14:43:22.157011" + "timestamp": "2024-01-09T23:01:18.710682" } -} +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index b9e8f926..1f21c664 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -3,24 +3,20 @@ nextflow_process { name "Test Process FASTQC" script "../main.nf" process "FASTQC" + tag "modules" tag "modules_nfcore" tag "fastqc" - test("Single-Read") { + test("sarscov2 single-end [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ - input[0] = [ + input[0] = Channel.of([ [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) """ } } @@ -28,82 +24,189 @@ nextflow_process { then { assertAll ( { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. // looks like this:
Mon 2 Oct 2023
test.gz
// https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, - { assert path(process.out.html.get(0).get(1)).getText().contains("") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 paired-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 interleaved [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } ) } } -// TODO -// // -// // Test with paired-end data -// // -// workflow test_fastqc_paired_end { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with interleaved data -// // -// workflow test_fastqc_interleaved { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with bam data -// // -// workflow test_fastqc_bam { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with multiple samples -// // -// workflow test_fastqc_multiple { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with custom prefix -// // -// workflow test_fastqc_custom_prefix { -// input = [ -// [ id:'mysample', single_end:true ], // meta map -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } + + test("sarscov2 paired-end [bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 multiple [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + { assert path(process.out.html[0][1][2]).text.contains("") }, + { assert path(process.out.html[0][1][3]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 custom_prefix") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastq] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.html.collect { file(it[1]).getName() } + + process.out.zip.collect { file(it[1]).getName() } + + process.out.versions ).match() } + ) + } + } + } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 636a32ce..5d624bb8 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,10 +1,20 @@ { + "sarscov2 single-end [fastq] - stub": { + "content": [ + [ + "test.html", + "test.zip", + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2024-01-17T18:40:57.254299" + }, "versions": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2023-10-09T23:40:54+0000" + "timestamp": "2024-01-17T18:36:50.033627" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index bc0bdb5b..7625b752 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.18 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 00cc48d2..1b9f7c43 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : - 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -43,7 +43,7 @@ process MULTIQC { stub: """ - touch multiqc_data + mkdir multiqc_data touch multiqc_plots touch multiqc_report.html diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f1aa660e..45a9bc35 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,4 +1,3 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index c2dad217..d0438eda 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -7,12 +7,9 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" - test("MULTIQC: FASTQC") { + test("sarscov2 single-end [fastqc]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -26,20 +23,17 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } ) } } - test("MULTIQC: FASTQC and a config file") { + test("sarscov2 single-end [fastqc] [config]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -53,9 +47,35 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastqc] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.report.collect { file(it).getName() } + + process.out.data.collect { file(it).getName() } + + process.out.plots.collect { file(it).getName() } + + process.out.versions ).match() } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap new file mode 100644 index 00000000..d37e7304 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -0,0 +1,21 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:02:49.911994" + }, + "sarscov2 single-end [fastqc] - stub": { + "content": [ + [ + "multiqc_report.html", + "multiqc_data", + "multiqc_plots", + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:03:14.524346" + } +} \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 37b455f2..d9fae6c8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -71,7 +71,7 @@ try { } // Load nf-core/crisprseq custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs! +// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! // try { // includeConfig "${params.custom_config_base}/pipeline/crisprseq.config" // } catch (Exception e) { @@ -91,6 +91,7 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false + channels = ['conda-forge', 'bioconda', 'defaults'] apptainer.enabled = false } mamba { @@ -233,7 +234,7 @@ manifest { description = """Pipeline for the analysis of CRISPR data""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '2.1.1' + version = '2.2.0dev' doi = '' } diff --git a/pyproject.toml b/pyproject.toml index 0d62beb6..7d08e1c8 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,10 +1,13 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] +[tool.ruff] line-length = 120 -target_version = ["py37", "py38", "py39", "py310"] +target-version = "py38" +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] +cache-dir = "~/.cache/ruff" -[tool.isort] -profile = "black" -known_first_party = ["nf_core"] -multi_line_output = 3 +[tool.ruff.isort] +known-first-party = ["nf_core"] + +[tool.ruff.per-file-ignores] +"__init__.py" = ["E402", "F401"] diff --git a/workflows/crisprseq.nf b/workflows/crisprseq.nf index 0cc1d334..d49e6e2b 100644 --- a/workflows/crisprseq.nf +++ b/workflows/crisprseq.nf @@ -127,6 +127,13 @@ workflow.onComplete { } } +workflow.onError { + if (workflow.errorReport.contains("Process requirement exceeds available memory")) { + println("🛑 Default resources exceed availability 🛑 ") + println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") + } +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END From b6bce4b61765b2429106a58a8623cab761fc9d94 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Wed, 31 Jan 2024 10:47:18 +0100 Subject: [PATCH 037/226] Fix bagel2 --- workflows/crisprseq_screening.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 8eb56572..a00bbc02 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -210,8 +210,8 @@ workflow CRISPRSEQ_SCREENING { counts = ch_bagel.combine(ch_counts) //Define non essential and essential genes channels for bagel2 - ch_bagel_reference_essentials= Channel.value(params.bagel_reference_essentials) - ch_bagel_reference_nonessentials= Channel.value(params.bagel_reference_nonessentials) + ch_bagel_reference_essentials= Channel.fromPath(params.bagel_reference_essentials).first() + ch_bagel_reference_nonessentials= Channel.fromPath(params.bagel_reference_nonessentials).first() BAGEL2_FC ( counts From 67a5f28291b851af7e1b284645536fc34a5555fd Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 31 Jan 2024 10:37:16 +0000 Subject: [PATCH 038/226] [automated] Fix linting with Prettier --- .devcontainer/devcontainer.json | 8 ++++---- CHANGELOG.md | 3 +-- docs/usage/screening.md | 6 ++---- 3 files changed, 7 insertions(+), 10 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe3..4a9bc5c7 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,11 +18,11 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.linting.pylintPath": "/opt/conda/bin/pylint", }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] - } - } + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], + }, + }, } diff --git a/CHANGELOG.md b/CHANGELOG.md index 682c8d01..7e1f9303 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,7 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Added - Update all modules to the last version in nf-core/modules ([#92](https://github.com/nf-core/crisprseq/pull/92)) -- More documentation for screening analysis. ([#99](https://github.com/nf-core/crisprseq/pull/99)) +- More documentation for screening analysis. ([#99](https://github.com/nf-core/crisprseq/pull/99)) - Contrasts are now given under a different flag and MAGeCK MLE and BAGEL2 are automatically run instead of MAGeCK RRA. ([#99](https://github.com/nf-core/crisprseq/pull/99)) - Added cutadapt for screening analysis ([#95](https://github.com/nf-core/crisprseq/pull/95)) @@ -18,7 +18,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Fixed paired-end for screening analysis ([#94](https://github.com/nf-core/crisprseq/pull/94)) - ## [v2.1.0 - Jamon Salas](https://github.com/nf-core/crisprseq/releases/tag/2.1.0) - [14.11.2023] ### Added diff --git a/docs/usage/screening.md b/docs/usage/screening.md index 18fcf36d..b0c6dcac 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -21,7 +21,7 @@ nextflow run nf-core/crisprseq --analysis screening --input samplesheet.csv --li ``` The following required parameters are here described. - If you wish to input a raw count or normalized table, you can skip the samplesheet parameter as well as the library one and directly input your table using count_table `--count_table your_count_table`. If your count table is normalized, be sure to set the normalization method to none in MAGeCK MLE or MAGeCK RRA using a config file. +If you wish to input a raw count or normalized table, you can skip the samplesheet parameter as well as the library one and directly input your table using count_table `--count_table your_count_table`. If your count table is normalized, be sure to set the normalization method to none in MAGeCK MLE or MAGeCK RRA using a config file. ### Full samplesheet @@ -42,7 +42,6 @@ SRR8983580,SRR8983580.small.fastq.gz,,treatment An [example samplesheet](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/samplesheet_test.csv) has been provided with the pipeline. - ### cutadapt MAGeCK count which is the main alignment software used is normally able to automatically determine the trimming length and sgRNA length, in most cases. Therefore, you don't need to go to this step unless MAGeCK fails to do so by itself. If the nucleotide length in front of sgRNA varies between different reads, you can use cutadapt to remove the adaptor sequences by using the flag `--cutadapt ADAPTER`. @@ -61,7 +60,6 @@ We recommend to run MAGeCK MLE and BAGEL2 as these are the most used and most re CRISPRcleanR is used for gene count normalization and the removal of biases for genomic segments for which copy numbers are amplified. Currently, the pipeline only supports annotation libraries already present in the R package and which can be found [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). - ### Running MAGeCK MLE and BAGEL2 with a contrast file To run both MAGeCK MLE and BAGEL2, you can provide a contrast file with the flag `--contrasts` with the mandatory headers "treatment" and "reference". These two columns should be separated with a dot comma (;) and contain the `csv` extension. You can also integrate several samples/conditions by comma separating them in each column. Please find an example here below : @@ -75,7 +73,7 @@ A full example can be found [here](https://raw.githubusercontent.com/nf-core/tes ### Running MAGeCK RRA only -MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section and also specify `--rra` . +MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section and also specify `--rra` . ### Running MAGeCK MLE only From 2bdb9f8c1999016a5a6c4fe552cda03a0a63214e Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Wed, 31 Jan 2024 12:37:21 +0100 Subject: [PATCH 039/226] Ran prettier --- CHANGELOG.md | 3 +-- docs/usage/screening.md | 6 ++---- 2 files changed, 3 insertions(+), 6 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 682c8d01..7e1f9303 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,7 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Added - Update all modules to the last version in nf-core/modules ([#92](https://github.com/nf-core/crisprseq/pull/92)) -- More documentation for screening analysis. ([#99](https://github.com/nf-core/crisprseq/pull/99)) +- More documentation for screening analysis. ([#99](https://github.com/nf-core/crisprseq/pull/99)) - Contrasts are now given under a different flag and MAGeCK MLE and BAGEL2 are automatically run instead of MAGeCK RRA. ([#99](https://github.com/nf-core/crisprseq/pull/99)) - Added cutadapt for screening analysis ([#95](https://github.com/nf-core/crisprseq/pull/95)) @@ -18,7 +18,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Fixed paired-end for screening analysis ([#94](https://github.com/nf-core/crisprseq/pull/94)) - ## [v2.1.0 - Jamon Salas](https://github.com/nf-core/crisprseq/releases/tag/2.1.0) - [14.11.2023] ### Added diff --git a/docs/usage/screening.md b/docs/usage/screening.md index 18fcf36d..b0c6dcac 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -21,7 +21,7 @@ nextflow run nf-core/crisprseq --analysis screening --input samplesheet.csv --li ``` The following required parameters are here described. - If you wish to input a raw count or normalized table, you can skip the samplesheet parameter as well as the library one and directly input your table using count_table `--count_table your_count_table`. If your count table is normalized, be sure to set the normalization method to none in MAGeCK MLE or MAGeCK RRA using a config file. +If you wish to input a raw count or normalized table, you can skip the samplesheet parameter as well as the library one and directly input your table using count_table `--count_table your_count_table`. If your count table is normalized, be sure to set the normalization method to none in MAGeCK MLE or MAGeCK RRA using a config file. ### Full samplesheet @@ -42,7 +42,6 @@ SRR8983580,SRR8983580.small.fastq.gz,,treatment An [example samplesheet](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/samplesheet_test.csv) has been provided with the pipeline. - ### cutadapt MAGeCK count which is the main alignment software used is normally able to automatically determine the trimming length and sgRNA length, in most cases. Therefore, you don't need to go to this step unless MAGeCK fails to do so by itself. If the nucleotide length in front of sgRNA varies between different reads, you can use cutadapt to remove the adaptor sequences by using the flag `--cutadapt ADAPTER`. @@ -61,7 +60,6 @@ We recommend to run MAGeCK MLE and BAGEL2 as these are the most used and most re CRISPRcleanR is used for gene count normalization and the removal of biases for genomic segments for which copy numbers are amplified. Currently, the pipeline only supports annotation libraries already present in the R package and which can be found [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). - ### Running MAGeCK MLE and BAGEL2 with a contrast file To run both MAGeCK MLE and BAGEL2, you can provide a contrast file with the flag `--contrasts` with the mandatory headers "treatment" and "reference". These two columns should be separated with a dot comma (;) and contain the `csv` extension. You can also integrate several samples/conditions by comma separating them in each column. Please find an example here below : @@ -75,7 +73,7 @@ A full example can be found [here](https://raw.githubusercontent.com/nf-core/tes ### Running MAGeCK RRA only -MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section and also specify `--rra` . +MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section and also specify `--rra` . ### Running MAGeCK MLE only From b82c6b20f1c2a273c80722e2f26ac487b1d702c8 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Tue, 6 Feb 2024 13:19:25 +0100 Subject: [PATCH 040/226] ran nf-core modules patch --- .../normalize/crisprcleanr-normalize.diff | 64 ++++++++++++------- modules/nf-core/mageck/mle/mageck-mle.diff | 14 +++- 2 files changed, 53 insertions(+), 25 deletions(-) diff --git a/modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff b/modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff index 50dc1a76..daa9446f 100644 --- a/modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff +++ b/modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff @@ -1,12 +1,14 @@ Changes in module 'nf-core/crisprcleanr/normalize' --- modules/nf-core/crisprcleanr/normalize/main.nf +++ modules/nf-core/crisprcleanr/normalize/main.nf -@@ -8,12 +8,13 @@ +@@ -8,12 +8,15 @@ 'biocontainers/r-crisprcleanr:3.0.0--r42hdfd78af_1' }" input: - tuple val(meta), path(count_file), path(library_file) -+ tuple val(meta), path(count_file), val(library_file) ++ tuple val(meta), path(count_file) ++ val(library_value) ++ path(library_file) val(min_reads) val(min_targeted_genes) @@ -16,42 +18,58 @@ Changes in module 'nf-core/crisprcleanr/normalize' path "versions.yml", emit: versions when: -@@ -26,20 +27,32 @@ +@@ -26,20 +29,48 @@ """ #!/usr/bin/env Rscript library(CRISPRcleanR) - library <- read.delim('${library_file}', header=T,sep="\t") - row.names(library) <- library[["CODE"]] - normANDfcs <- ccr.NormfoldChanges('${count_file}',saveToFig = FALSE,min_reads=${min_reads},EXPname='${meta.id}', libraryAnnotation=library,display=FALSE) -- gwSortedFCs <- ccr.logFCs2chromPos(normANDfcs[["logFCs"]],library) -- correctedFCs <- ccr.GWclean(gwSortedFCs,display=FALSE,label='${meta.id}') -- correctedCounts <- ccr.correctCounts('${meta.id}', + library(dplyr) -+ data('${library_file}') -+ count_file <- read.delim('${count_file}',header=T,sep = "\t") -+ count_file_to_normalize <- count_file %>% dplyr::left_join(get('${library_file}'), by=c("sgRNA"="Target.Context.Sequence"),multiple = "all") + -+ count_file_to_normalize <- count_file_to_normalize %>% -+ dplyr::select(colnames(count_file),CODE,-sgRNA) ++ print('${library_value}') ++ count_file <- read.delim('${count_file}',header=T,sep = "\t") ++ count_file <- count_file[!duplicated(count_file\$sgRNA), ] ++ if('${library_file}' == "") { ++ data('${library_value}') ++ library <- as.data.frame(get('${library_value}')) ++ #colnames(library) ++ #print(head(count_file)) ++ #print(head(library)) ++ count_file_to_normalize <- count_file %>% dplyr::left_join(library, by=c("sgRNA"="seq"),multiple = "all") ++ count_file_to_normalize <- count_file_to_normalize %>% ++ dplyr::select(colnames(count_file),CODE,-sgRNA) + -+ names(count_file_to_normalize)[names(count_file_to_normalize) == 'Gene'] <- 'gene' -+ names(count_file_to_normalize)[names(count_file_to_normalize) == 'CODE'] <- 'sgRNA' -+ count_file_to_normalize <- count_file_to_normalize %>% dplyr::select(sgRNA, gene, everything()) ++ names(count_file_to_normalize)[names(count_file_to_normalize) == 'Gene'] <- 'gene' ++ names(count_file_to_normalize)[names(count_file_to_normalize) == 'CODE'] <- 'sgRNA' ++ count_file_to_normalize <- count_file_to_normalize %>% dplyr::select(sgRNA, gene, everything()) ++ } else { ++ try(library <- read.delim('${library_file}',header=T,sep = ",")) ++ duplicates <- duplicated(library[, 1]) ++ unique_rows <- !duplicates ++ library <- library[unique_rows, , drop = FALSE] ++ rownames(library) = library[,1] ++ library = library[order(rownames(library)),] ++ library = library[,-1] ++ count_file_to_normalize <- count_file ++ } + -+ #crisprcleanr function -+ normANDfcs <- ccr.NormfoldChanges(Dframe=count_file_to_normalize,saveToFig = FALSE,min_reads=${min_reads},EXPname="${prefix}", libraryAnnotation=get('${library_file}'),display=FALSE) -+ gwSortedFCs <- ccr.logFCs2chromPos(normANDfcs[["logFCs"]],get('${library_file}')) -+ correctedFCs <- ccr.GWclean(gwSortedFCs,display=FALSE,label='${meta}') -+ correctedCounts <- ccr.correctCounts('${meta}', ++ normANDfcs <- ccr.NormfoldChanges(Dframe=count_file_to_normalize,saveToFig = FALSE,min_reads=${min_reads},EXPname="${prefix}", libraryAnnotation=library,display=FALSE) + gwSortedFCs <- ccr.logFCs2chromPos(normANDfcs[["logFCs"]],library) +- correctedFCs <- ccr.GWclean(gwSortedFCs,display=FALSE,label='${meta.id}') +- correctedCounts <- ccr.correctCounts('${meta.id}', ++ correctedFCs <- ccr.GWclean(gwSortedFCs,display=FALSE,label='crisprcleanr') ++ correctedCounts <- ccr.correctCounts('crisprcleanr', normANDfcs[["norm_counts"]], correctedFCs, -- library, -+ get('${library_file}'), + library, minTargetedGenes=${min_targeted_genes}, OutDir='./') - write.table(correctedCounts, file=paste0("${prefix}","_norm_table.tsv"),row.names=FALSE,quote=FALSE,sep="\t") - +- write.table(correctedCounts, file=paste0("${prefix}","_norm_table.tsv"),row.names=FALSE,quote=FALSE,sep="\t") +- ++ write.table(correctedCounts, file=paste0("crisprcleanr","_norm_table.tsv"),row.names=FALSE,quote=FALSE,sep="\t") ++ + #version version_file_path <- "versions.yml" version_crisprcleanr <- paste(unlist(packageVersion("CRISPRcleanR")), collapse = ".") diff --git a/modules/nf-core/mageck/mle/mageck-mle.diff b/modules/nf-core/mageck/mle/mageck-mle.diff index f5d5c324..485ceb04 100644 --- a/modules/nf-core/mageck/mle/mageck-mle.diff +++ b/modules/nf-core/mageck/mle/mageck-mle.diff @@ -3,8 +3,9 @@ Changes in module 'nf-core/mageck/mle' +++ modules/nf-core/mageck/mle/main.nf @@ -1,6 +1,6 @@ process MAGECK_MLE { - tag "$meta.id" +- tag "$meta.id" - label 'process_medium' ++ tag "${meta.treatment}_vs_${meta.reference}" + label 'process_high' conda "${moduleDir}/environment.yml" @@ -15,9 +16,18 @@ Changes in module 'nf-core/mageck/mle' input: - tuple val(meta), path(count_table) - path(design_matrix) -+ tuple val(meta), path(count_table), path(design_matrix) ++ tuple val(meta), path(design_matrix), path(count_table) output: tuple val(meta), path("*.gene_summary.txt") , emit: gene_summary +@@ -30,7 +29,7 @@ + --threads $task.cpus \\ + -k $count_table \\ + -d $design_matrix \\ +- -n $prefix ++ -n "${meta.treatment}_vs_${meta.reference}" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": ************************************************************ From e3f4c103cf9530db0c27362e0b2e33d591e9c063 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Wed, 7 Feb 2024 09:28:19 +0100 Subject: [PATCH 041/226] Adding the design_matrice option --- docs/usage/screening.md | 2 +- modules/local/bagel2/bf.nf | 6 +-- modules/local/bagel2/pr.nf | 2 +- modules/nf-core/mageck/mle/mageck-mle.diff | 19 ++++---- modules/nf-core/mageck/mle/main.nf | 7 +-- workflows/crisprseq_screening.nf | 56 +++++++++------------- 6 files changed, 42 insertions(+), 50 deletions(-) diff --git a/docs/usage/screening.md b/docs/usage/screening.md index b4b7c11b..82e5df0c 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -83,7 +83,7 @@ A full example can be found [here](https://raw.githubusercontent.com/nf-core/tes ### Running MAGeCK RRA only -MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section and also specify `--rra` . +MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section (with a `.txt` extension) and also specify `--rra` . ### Running MAGeCK MLE only diff --git a/modules/local/bagel2/bf.nf b/modules/local/bagel2/bf.nf index 91ab780c..e2db4e55 100644 --- a/modules/local/bagel2/bf.nf +++ b/modules/local/bagel2/bf.nf @@ -11,8 +11,8 @@ process BAGEL2_BF { input: tuple val(meta), path(foldchange) - val(reference_essentials) - val(reference_nonessentials) + path(reference_essentials) + path(reference_nonessentials) output: tuple val(meta), path("*.bf"), emit: bf @@ -23,7 +23,7 @@ process BAGEL2_BF { script: def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.treatment}" + def prefix = task.ext.prefix ?: "${meta.treatment}_vs_${meta.reference}" """ BAGEL.py bf -i $foldchange -o '${meta.treatment}_vs_${meta.reference}.bf' $args -e $reference_essentials -n $reference_nonessentials -c ${meta.treatment} diff --git a/modules/local/bagel2/pr.nf b/modules/local/bagel2/pr.nf index e2290ede..53a1f2fb 100644 --- a/modules/local/bagel2/pr.nf +++ b/modules/local/bagel2/pr.nf @@ -1,5 +1,5 @@ process BAGEL2_PR { - tag "$meta.treatment" + tag "${meta.treatment}_vs_${meta.reference}" label 'process_single' conda "python=3.11.4 pandas=2.0.3 numpy=1.25.1 scikit-learn=1.3.0 click=8.1.6" diff --git a/modules/nf-core/mageck/mle/mageck-mle.diff b/modules/nf-core/mageck/mle/mageck-mle.diff index 485ceb04..c830b3fe 100644 --- a/modules/nf-core/mageck/mle/mageck-mle.diff +++ b/modules/nf-core/mageck/mle/mageck-mle.diff @@ -5,7 +5,7 @@ Changes in module 'nf-core/mageck/mle' process MAGECK_MLE { - tag "$meta.id" - label 'process_medium' -+ tag "${meta.treatment}_vs_${meta.reference}" ++ tag "$prefix" + label 'process_high' conda "${moduleDir}/environment.yml" @@ -20,14 +20,15 @@ Changes in module 'nf-core/mageck/mle' output: tuple val(meta), path("*.gene_summary.txt") , emit: gene_summary -@@ -30,7 +29,7 @@ - --threads $task.cpus \\ - -k $count_table \\ - -d $design_matrix \\ -- -n $prefix -+ -n "${meta.treatment}_vs_${meta.reference}" +@@ -21,7 +20,8 @@ - cat <<-END_VERSIONS > versions.yml - "${task.process}": + script: + def args = task.ext.args ?: '' +- def prefix = task.ext.prefix ?: "${meta.id}" ++ prefix = meta.id ?: "${meta.treatment}_vs_${meta.treatment}" ++ + + """ + mageck \\ ************************************************************ diff --git a/modules/nf-core/mageck/mle/main.nf b/modules/nf-core/mageck/mle/main.nf index 462274ae..4dbe4953 100644 --- a/modules/nf-core/mageck/mle/main.nf +++ b/modules/nf-core/mageck/mle/main.nf @@ -1,5 +1,5 @@ process MAGECK_MLE { - tag "${meta.treatment}_vs_${meta.reference}" + tag "$prefix" label 'process_high' conda "${moduleDir}/environment.yml" @@ -20,7 +20,8 @@ process MAGECK_MLE { script: def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" + prefix = meta.id ?: "${meta.treatment}_vs_${meta.treatment}" + """ mageck \\ @@ -29,7 +30,7 @@ process MAGECK_MLE { --threads $task.cpus \\ -k $count_table \\ -d $design_matrix \\ - -n "${meta.treatment}_vs_${meta.reference}" + -n $prefix cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 797e5443..4b63faee 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -53,8 +53,16 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT LOCAL MODULES/SUBWORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + + */ +include { BAGEL2_FC } from '../modules/local/bagel2/fc' +include { BAGEL2_BF } from '../modules/local/bagel2/bf' +include { BAGEL2_PR } from '../modules/local/bagel2/pr' +include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' +include { MATRICESCREATION } from '../modules/local/matricescreation' + // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // @@ -68,20 +76,16 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // MODULE: Installed directly from nf-core/modules // -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { CUTADAPT } from '../modules/nf-core/cutadapt/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { MAGECK_COUNT } from '../modules/nf-core/mageck/count/main' -include { MAGECK_MLE } from '../modules/nf-core/mageck/mle/main' -include { MAGECK_TEST } from '../modules/nf-core/mageck/test/main' -include { MAGECK_GRAPHRRA } from '../modules/local/mageck/graphrra' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' -include { CRISPRCLEANR_NORMALIZE } from '../modules/nf-core/crisprcleanr/normalize/main' -include { BAGEL2_FC } from '../modules/local/bagel2/fc' -include { BAGEL2_BF } from '../modules/local/bagel2/bf' -include { BAGEL2_PR } from '../modules/local/bagel2/pr' -include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' -include { MATRICESCREATION } from '../modules/local/matricescreation' +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { CUTADAPT } from '../modules/nf-core/cutadapt/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { MAGECK_COUNT } from '../modules/nf-core/mageck/count/main' +include { MAGECK_MLE } from '../modules/nf-core/mageck/mle/main' +include { MAGECK_TEST } from '../modules/nf-core/mageck/test/main' +include { MAGECK_GRAPHRRA } from '../modules/local/mageck/graphrra' +include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' +include { CRISPRCLEANR_NORMALIZE } from '../modules/nf-core/crisprcleanr/normalize/main' +include { MAGECK_MLE as MAGECK_MLE_MATRIX } from '../modules/nf-core/mageck/mle/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -115,7 +119,6 @@ workflow CRISPRSEQ_SCREENING { ) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - ch_input_cutadapt = ch_input.combine(Channel.value([[]])) if(params.cutadapt) { @@ -132,7 +135,6 @@ workflow CRISPRSEQ_SCREENING { } // this is to concatenate everything for mageck count - ch_input .map { meta, fastqs -> if(fastqs.size() == 1){ @@ -228,8 +230,8 @@ workflow CRISPRSEQ_SCREENING { counts = ch_contrasts.combine(ch_counts) //Define non essential and essential genes channels for bagel2 - ch_bagel_reference_essentials= Channel.value(params.bagel_reference_essentials) - ch_bagel_reference_nonessentials= Channel.value(params.bagel_reference_nonessentials) + ch_bagel_reference_essentials= Channel.fromPath(params.bagel_reference_essentials).first() + ch_bagel_reference_nonessentials= Channel.fromPath(params.bagel_reference_nonessentials).first() BAGEL2_FC ( counts @@ -265,10 +267,12 @@ workflow CRISPRSEQ_SCREENING { if((params.mle_design_matrix) || (params.contrasts && !params.rra)) { //TODO FINISH THIS PART!! If you see this in a PR please point it out to me if(params.mle_design_matrix) { - ch_mle = ch_design.combine.ch_counts ch_design.map { it -> [[id: it.getBaseName()], it] }.set { ch_designed_test } + ch_mle = ch_designed_test.combine(ch_counts) + ch_mle.dump(tag: "ch_mle") + MAGECK_MLE_MATRIX (ch_mle) } if(params.contrasts) { MATRICESCREATION(ch_contrasts) @@ -276,20 +280,6 @@ workflow CRISPRSEQ_SCREENING { MAGECK_MLE (ch_mle) ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) } - // ch_test.map { - // it -> [[id: it[0].getBaseName()], it] - //}.set { ch_designed_test } - //ch_designed_test.dump(tag: "ch_test design") - // ch_mle.map { - // it -> [[id: it[1].getBaseName()], it[0], it[1]] - //}.set { ch_designed_mle } - - // MAGECK_MLE ( - // ch_designed_mle - //) - - // - } CUSTOM_DUMPSOFTWAREVERSIONS ( From c0ee52c4e59358b2c67a2f1aa72adca854844576 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Wed, 7 Feb 2024 09:30:42 +0100 Subject: [PATCH 042/226] Modified changelog --- CHANGELOG.md | 3 +++ 1 file changed, 3 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 66fcc36f..589d56a5 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,6 +8,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Added - Template update to 2.11.1 ([#105](https://github.com/nf-core/crisprseq/pull/105)) +- Added a csv input option for crisprcleanr ([#105](https://github.com/nf-core/crisprseq/pull/105)) +- Added a contrasts parameter so the pipeline automatically creates design matrices and MAGeCK MLE ([#109](https://github.com/nf-core/crisprseq/pull/109)) + ## [v2.1.1 - Jamon Salas - patch](https://github.com/nf-core/crisprseq/releases/tag/2.1.1) - [14.12.2023] From 4fc737a31215df520ffdb2d3c4222345fb545389 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Fri, 9 Feb 2024 14:58:38 +0100 Subject: [PATCH 043/226] Apply suggestions from code review MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Júlia Mir Pedrol --- workflows/crisprseq_screening.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 4b63faee..6ae562e0 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -22,8 +22,8 @@ if (params.crisprcleanr) { ch_crisprcleanr_file = file(params.crisprcleanr) } else { ch_crisprcleanr_value = Channel.value(params.crisprcleanr) - } } +} if(params.mle_design_matrix) { Channel.fromPath(params.mle_design_matrix) From ec303659e3201cc51e5179a511ebae0835f2a74d Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Fri, 9 Feb 2024 15:04:25 +0100 Subject: [PATCH 044/226] Update workflows/crisprseq_screening.nf --- workflows/crisprseq_screening.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 6ae562e0..44c141cd 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -265,7 +265,6 @@ workflow CRISPRSEQ_SCREENING { } if((params.mle_design_matrix) || (params.contrasts && !params.rra)) { - //TODO FINISH THIS PART!! If you see this in a PR please point it out to me if(params.mle_design_matrix) { ch_design.map { it -> [[id: it.getBaseName()], it] From 1a80acd057ad9cd8336e8735db35e1394242bbe8 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Fri, 9 Feb 2024 15:06:18 +0100 Subject: [PATCH 045/226] Update workflows/crisprseq_screening.nf --- workflows/crisprseq_screening.nf | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 44c141cd..e000a907 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -268,9 +268,8 @@ workflow CRISPRSEQ_SCREENING { if(params.mle_design_matrix) { ch_design.map { it -> [[id: it.getBaseName()], it] - }.set { ch_designed_test } - ch_mle = ch_designed_test.combine(ch_counts) - ch_mle.dump(tag: "ch_mle") + }.set { ch_designed_mle } + ch_mle = ch_designed_mle.combine(ch_counts) MAGECK_MLE_MATRIX (ch_mle) } if(params.contrasts) { From a8e15bf2deabfe0fabae2cf1246252c34934acc4 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Fri, 9 Feb 2024 15:56:54 +0100 Subject: [PATCH 046/226] Add example in docs --- docs/usage/screening.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage/screening.md b/docs/usage/screening.md index 82e5df0c..a455a871 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -21,7 +21,7 @@ nextflow run nf-core/crisprseq --analysis screening --input samplesheet.csv --li ``` The following required parameters are here described. -If you wish to input a raw count or normalized table, you can skip the samplesheet parameter as well as the library one and directly input your table using count_table `--count_table your_count_table`. Your count table should contain the following columns : sgRNA and gene. If your count table is normalized, be sure to set the normalization method to none in MAGeCK MLE or MAGeCK RRA using a config file. +If you wish to input a raw count or normalized table, you can skip the samplesheet parameter as well as the library one and directly input your table using count_table `--count_table your_count_table`. Your count table should contain the following columns : sgRNA and gene. You can find an example [here](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/count_table.csv) If your count table is normalized, be sure to set the normalization method to none in MAGeCK MLE or MAGeCK RRA using a config file. ### Full samplesheet From 3481769d8387e6da84748dd745362114351e872a Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Tue, 13 Feb 2024 09:27:58 +0100 Subject: [PATCH 047/226] fix download_pipeline GHA --- .github/workflows/download_pipeline.yml | 9 +++++++-- 1 file changed, 7 insertions(+), 2 deletions(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458a..f560a627 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -6,6 +6,11 @@ name: Test successful pipeline download with 'nf-core download' # - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. on: workflow_dispatch: + inputs: + testbranch: + description: "The specific branch you wish to utilize for the test execution of nf-core download." + required: true + default: "dev" pull_request: types: - opened @@ -42,7 +47,7 @@ jobs: run: | echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} - echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + echo "{% raw %}REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} - name: Download the pipeline env: @@ -64,4 +69,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results {% endraw %} From 1d22c25a997d9c4d7536601bd7da70b69abd8b56 Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Tue, 13 Feb 2024 09:34:33 +0100 Subject: [PATCH 048/226] fix deafult values --- nextflow_schema.json | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 0e551a9e..1fc7ad67 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -82,7 +82,7 @@ }, "medaka_model": { "type": "string", - "default": "r941_min_high_g360", + "default": "r941_min_high_g303", "fa_icon": "fas fa-font", "description": "Medaka model (-m) to use according to the basecaller used." } @@ -429,7 +429,7 @@ }, "validationSchemaIgnoreParams": { "type": "string", - "default": "genomes", + "default": "genomes,igenomes_base", "description": "Ignore JSON schema validation of the following params", "fa_icon": "fas fa-ban", "hidden": true From cafc55e4ac344514748c6b7e761b2f6d0398954c Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Tue, 13 Feb 2024 09:46:59 +0100 Subject: [PATCH 049/226] run prettier --- .devcontainer/devcontainer.json | 8 +- bin/BAGEL.py | 235 +++++++++++++----- bin/extract_umis.py | 42 +++- .../templates/dumpsoftwareversions.py | 4 +- templates/alignment_summary.py | 8 +- templates/preprocessing_summary.py | 8 +- 6 files changed, 223 insertions(+), 82 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4a9bc5c7..4ecfbfe3 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,11 +18,11 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint", + "python.linting.pylintPath": "/opt/conda/bin/pylint" }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], - }, - }, + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] + } + } } diff --git a/bin/BAGEL.py b/bin/BAGEL.py index 205fbb44..aa2f5f97 100755 --- a/bin/BAGEL.py +++ b/bin/BAGEL.py @@ -209,8 +209,8 @@ def report_bagel_version(): """ print( "Bayesian Analysis of Gene EssentiaLity (BAGEL) suite:\n" - "Version: {VERSION}\n" - "Build: {BUILD}".format(VERSION=VERSION, BUILD=BUILD) + f"Version: {VERSION}\n" + f"Build: {BUILD}" ) @@ -220,7 +220,9 @@ def report_bagel_version(): @click.option("-c", "--control-columns", required=True) @click.option("-m", "--min-reads", type=int, default=0) @click.option("-Np", "--pseudo-count", type=int, default=5) -def calculate_fold_change(read_count_file, output_label, control_columns, min_reads, pseudo_count): +def calculate_fold_change( + read_count_file, output_label, control_columns, min_reads, pseudo_count +): """ \b Calculate fold changes from read count data outputting a fold change column: @@ -294,7 +296,9 @@ def calculate_fold_change(read_count_file, output_label, control_columns, min_re normed = pd.DataFrame(index=reads.index.values) normed["GENE"] = reads.iloc[:, 0] # first column is gene name normed = ( - reads.iloc[:, list(range(1, numColumns))] / np.tile(sumReads, [numClones, 1]) * 10000000 + reads.iloc[:, list(range(1, numColumns))] + / np.tile(sumReads, [numClones, 1]) + * 10000000 ) # normalize to 10M reads # @@ -334,14 +338,26 @@ def calculate_fold_change(read_count_file, output_label, control_columns, min_re @click.option("-i", "--fold-change", required=True, type=click.Path(exists=True)) @click.option("-o", "--output-file", required=True) @click.option("-e", "--essential-genes", required=True, type=click.Path(exists=True)) -@click.option("-n", "--non-essential-genes", required=True, type=click.Path(exists=True)) +@click.option( + "-n", "--non-essential-genes", required=True, type=click.Path(exists=True) +) @click.option("-c", "--columns-to-test", required=True) -@click.option("-w", "--network-file", metavar="[network File]", default=None, type=click.Path(exists=True)) +@click.option( + "-w", + "--network-file", + metavar="[network File]", + default=None, + type=click.Path(exists=True), +) @click.option("-m", "--filter-multi-target", is_flag=True) @click.option("-m0", "--loci-without-mismatch", type=int, default=10) @click.option("-m1", "--loci-with-mismatch", type=int, default=10) @click.option( - "--align-info", metavar="--align-info [File]", default=None, type=click.Path(exists=True), cls=OptionRequiredIf + "--align-info", + metavar="--align-info [File]", + default=None, + type=click.Path(exists=True), + cls=OptionRequiredIf, ) @click.option("-b", "--use-bootstrapping", is_flag=True) @click.option("-NS", "--no-resampling", is_flag=True) @@ -474,7 +490,9 @@ def calculate_bayes_factors( if filter_multi_target: try: - aligninfo = pd.read_csv(align_info, header=None, index_col=0, sep="\t").fillna("") + aligninfo = pd.read_csv( + align_info, header=None, index_col=0, sep="\t" + ).fillna("") for seqid in aligninfo.index: perfectmatch = 0 mismatch_1bp = 0 @@ -488,7 +506,10 @@ def calculate_bayes_factors( mismatch_1bp = len(aligninfo[3][seqid].split(",")) if aligninfo[4][seqid] != "": mismatch_1bp_gene = len(aligninfo[4][seqid].split(",")) - if perfectmatch > loci_without_mismatch or mismatch_1bp > loci_with_mismatch: + if ( + perfectmatch > loci_without_mismatch + or mismatch_1bp > loci_with_mismatch + ): multi_targeting_sgrnas[seqid] = True elif perfectmatch > 1 or mismatch_1bp > 0: multi_targeting_sgrnas_info[seqid] = ( @@ -502,7 +523,9 @@ def calculate_bayes_factors( print("Please check align-info file") sys.exit(1) - print("Total %d multi-targeting gRNAs are discarded" % len(multi_targeting_sgrnas)) + print( + "Total %d multi-targeting gRNAs are discarded" % len(multi_targeting_sgrnas) + ) # # LOAD FOLDCHANGES @@ -521,7 +544,9 @@ def calculate_bayes_factors( except ValueError: column_labels = columns column_list = [ - x for x in range(len(fieldname) - 1) if fieldname[x + 1] in column_labels + x + for x in range(len(fieldname) - 1) + if fieldname[x + 1] in column_labels ] # +1 because of First column start 2 print("Using column: " + ", ".join(column_labels)) # print "Using column: " + ", ".join(map(str,column_list)) @@ -549,7 +574,9 @@ def calculate_bayes_factors( rna2gene[rnatag] = gsym fc[rnatag] = {} for i in column_list: - fc[rnatag][i] = float(fields[i + 1]) # per user docs, GENE is column 0, first data column is col 1. + fc[rnatag][i] = float( + fields[i + 1] + ) # per user docs, GENE is column 0, first data column is col 1. genes_array = np.array(list(genes.keys())) gene_idx = np.arange(len(genes)) @@ -590,7 +617,9 @@ def calculate_bayes_factors( for i in [0, 1]: if linearray[i] not in network: network[linearray[i]] = {} - network[linearray[i]][linearray[-1 * (i - 1)]] = 1 # save edge information + network[linearray[i]][ + linearray[-1 * (i - 1)] + ] = 1 # save edge information edgecount += 1 print("Number of network edges: " + str(edgecount)) @@ -602,7 +631,9 @@ def calculate_bayes_factors( # Define foldchange dynamic threshold. logarithm decay. # Parameters are defined by regression (achilles data) 2**-7 was used in previous version. - FC_THRESH = 2 ** (-1.1535 * np.log(len(np.intersect1d(genes_array, nonEss)) + 13.324) + 0.7728) + FC_THRESH = 2 ** ( + -1.1535 * np.log(len(np.intersect1d(genes_array, nonEss)) + 13.324) + 0.7728 + ) bf = {} boostedbf = {} for g in genes_array: @@ -619,12 +650,17 @@ def calculate_bayes_factors( # training_data = Training(setdiff1d(gene_idx,np.where(in1d(genes_array,coreEss))),cvnum=NUMCV) # declare training class (only for Gold-standard gene set) training_data = Training( - np.where(np.in1d(genes_array, np.union1d(coreEss, nonEss)))[0], cvnum=no_of_cross_validations + np.where(np.in1d(genes_array, np.union1d(coreEss, nonEss)))[0], + cvnum=no_of_cross_validations, ) # all non-goldstandards - all_non_gs = np.where(np.logical_not(np.in1d(genes_array, np.union1d(coreEss, nonEss))))[0] + all_non_gs = np.where( + np.logical_not(np.in1d(genes_array, np.union1d(coreEss, nonEss))) + )[0] else: - training_data = Training(gene_idx, cvnum=no_of_cross_validations) # declare training class + training_data = Training( + gene_idx, cvnum=no_of_cross_validations + ) # declare training class if train_method == 0: LOOPCOUNT = bootstrap_iterations @@ -665,7 +701,12 @@ def calculate_bayes_factors( if run_test_mode: fp.write( - "%d\n%s\n%s\n" % (loop, ",".join(genes_array[gene_train_idx]), ",".join(genes_array[gene_test_idx])) + "%d\n%s\n%s\n" + % ( + loop, + ",".join(genes_array[gene_train_idx]), + ",".join(genes_array[gene_test_idx]), + ) ) train_ess = np.where(np.in1d(genes_array[gene_train_idx], coreEss))[0] @@ -679,16 +720,28 @@ def calculate_bayes_factors( # define ess_train: vector of observed fold changes of essential genes in training set # ess_train_fc_list_of_lists = [ - fc[rnatag] for g in genes_array[gene_train_idx[train_ess]] for rnatag in gene2rna[g] + fc[rnatag] + for g in genes_array[gene_train_idx[train_ess]] + for rnatag in gene2rna[g] + ] + ess_train_fc_flat_list = [ + obs + for sublist in ess_train_fc_list_of_lists + for obs in list(sublist.values()) ] - ess_train_fc_flat_list = [obs for sublist in ess_train_fc_list_of_lists for obs in list(sublist.values())] # # define non_train vector of observed fold changes of nonessential genes in training set # non_train_fc_list_of_lists = [ - fc[rnatag] for g in genes_array[gene_train_idx[train_non]] for rnatag in gene2rna[g] + fc[rnatag] + for g in genes_array[gene_train_idx[train_non]] + for rnatag in gene2rna[g] + ] + non_train_fc_flat_list = [ + obs + for sublist in non_train_fc_list_of_lists + for obs in list(sublist.values()) ] - non_train_fc_flat_list = [obs for sublist in non_train_fc_list_of_lists for obs in list(sublist.values())] # # calculate empirical fold change distributions for both # @@ -713,7 +766,9 @@ def calculate_bayes_factors( # logratio_lookup = {} for i in np.arange(xmin, xmax + 0.01, 0.01): - logratio_lookup[np.around(i * 100)] = np.log2(kess.evaluate(i) / knon.evaluate(i)) + logratio_lookup[np.around(i * 100)] = np.log2( + kess.evaluate(i) / knon.evaluate(i) + ) # # calculate BFs from lookup table for withheld test set # @@ -729,7 +784,9 @@ def calculate_bayes_factors( testx.append(np.around(foldchange * 100) / 100) testy.append(logratio_lookup[np.around(foldchange * 100)][0]) try: - slope, intercept, r_value, p_value, std_err = stats.linregress(np.array(testx), np.array(testy)) + slope, intercept, r_value, p_value, std_err = stats.linregress( + np.array(testx), np.array(testy) + ) except: print("Regression failed. Check quality of the screen") sys.exit(1) @@ -798,15 +855,23 @@ def calculate_bayes_factors( else: onlytarget.append(seqid) - if len(onlytarget) > 0: # comparsion between sgRNAs targeting one locus and multiple loci + if ( + len(onlytarget) > 0 + ): # comparsion between sgRNAs targeting one locus and multiple loci if len(multitarget) > 0: - bf_only = np.mean([sum(list(bf_mean_rna_rep[seqid].values())) for seqid in onlytarget]) + bf_only = np.mean( + [ + sum(list(bf_mean_rna_rep[seqid].values())) + for seqid in onlytarget + ] + ) for seqid in onlytarget: trainset[seqid] = [1, 0, 0] for seqid in multitarget: if ( - multi_targeting_sgrnas_info[seqid][2] > 1 or multi_targeting_sgrnas_info[seqid][3] > 0 + multi_targeting_sgrnas_info[seqid][2] > 1 + or multi_targeting_sgrnas_info[seqid][3] > 0 ): # train model using multi-targeting only targeting one protein coding gene continue @@ -821,7 +886,9 @@ def calculate_bayes_factors( if count < 10: print("Not enough train set for calculating multi-targeting effect.\n") - print("It may cause due to unmatched gRNA names between the foldchange file and the align info file.\n") + print( + "It may cause due to unmatched gRNA names between the foldchange file and the align info file.\n" + ) print("Filtering is not finished\n") filter_multi_target = False @@ -835,7 +902,9 @@ def calculate_bayes_factors( coeff_df = pd.DataFrame(regressor.coef_, X.columns, columns=["Coefficient"]) for i in [0, 1]: if coeff_df["Coefficient"][i] < 0: - print("Regression coefficient is below than zero. Substituted to zero\n") + print( + "Regression coefficient is below than zero. Substituted to zero\n" + ) coeff_df["Coefficient"][i] = 0.0 print( "Multiple effects from perfect matched loci = %.3f and 1bp mis-matched loci = %.3f" @@ -848,8 +917,10 @@ def calculate_bayes_factors( for seqid in gene2rna[g]: if seqid in multi_targeting_sgrnas_info: penalty += ( - float(multi_targeting_sgrnas_info[seqid][0] - 1) * coeff_df["Coefficient"][0] - + float(multi_targeting_sgrnas_info[seqid][1]) * coeff_df["Coefficient"][1] + float(multi_targeting_sgrnas_info[seqid][0] - 1) + * coeff_df["Coefficient"][0] + + float(multi_targeting_sgrnas_info[seqid][1]) + * coeff_df["Coefficient"][1] ) bf_multi_corrected_gene[g] = bf_mean[g] - penalty else: @@ -857,12 +928,16 @@ def calculate_bayes_factors( for seqid in gene2rna[g]: if seqid in multi_targeting_sgrnas_info: penalty = ( - float(multi_targeting_sgrnas_info[seqid][0] - 1) * coeff_df["Coefficient"][0] - + float(multi_targeting_sgrnas_info[seqid][1]) * coeff_df["Coefficient"][1] + float(multi_targeting_sgrnas_info[seqid][0] - 1) + * coeff_df["Coefficient"][0] + + float(multi_targeting_sgrnas_info[seqid][1]) + * coeff_df["Coefficient"][1] ) else: penalty = 0.0 - bf_multi_corrected_rna[seqid] = sum(list(bf_mean_rna_rep[seqid].values())) - penalty + bf_multi_corrected_rna[seqid] = ( + sum(list(bf_mean_rna_rep[seqid].values())) - penalty + ) # # NORMALIZE sgRNA COUNT @@ -889,7 +964,9 @@ def calculate_bayes_factors( # calculate network scores # - if network_boost == True and rna_level == False: # Network boost is only working for gene level + if ( + network_boost == True and rna_level == False + ): # Network boost is only working for gene level if run_test_mode == True: # TEST MODE fp = open(output_file + ".netscore", "w") print("\nNetwork score calculation start\n") @@ -931,8 +1008,16 @@ def calculate_bayes_factors( # # calculate Network BF for test set # - ess_ns_list = [networkscores[x] for x in genes_array[gene_train_idx[train_ess]] if x in networkscores] - non_ns_list = [networkscores[x] for x in genes_array[gene_train_idx[train_non]] if x in networkscores] + ess_ns_list = [ + networkscores[x] + for x in genes_array[gene_train_idx[train_ess]] + if x in networkscores + ] + non_ns_list = [ + networkscores[x] + for x in genes_array[gene_train_idx[train_non]] + if x in networkscores + ] kess = stats.gaussian_kde(ess_ns_list) knon = stats.gaussian_kde(non_ns_list) @@ -950,7 +1035,10 @@ def calculate_bayes_factors( if density_ess == 0.0 or density_non == 0.0: continue - if np.log2(density_ess / density_non) > -5 and networkscore < np.array(ess_ns_list).mean(): # reverse + if ( + np.log2(density_ess / density_non) > -5 + and networkscore < np.array(ess_ns_list).mean() + ): # reverse xmin = min(xmin, networkscore) for networkscore in np.arange(min(non_ns_list), max(non_ns_list), 0.01): @@ -958,7 +1046,10 @@ def calculate_bayes_factors( density_non = knon.evaluate(networkscore)[0] if density_ess == 0.0 or density_non == 0.0: continue - if np.log2(density_ess / density_non) < 5 and networkscore > np.array(non_ns_list).mean(): # reverse + if ( + np.log2(density_ess / density_non) < 5 + and networkscore > np.array(non_ns_list).mean() + ): # reverse xmax = max(xmax, networkscore) # # liner regression @@ -969,14 +1060,28 @@ def calculate_bayes_factors( if g in networkscores: if networkscores[g] >= xmin and networkscores[g] <= xmax: testx.append(np.around(networkscores[g] * 100) / 100) - testy.append(np.log2(kess.evaluate(networkscores[g])[0] / knon.evaluate(networkscores[g])[0])) + testy.append( + np.log2( + kess.evaluate(networkscores[g])[0] + / knon.evaluate(networkscores[g])[0] + ) + ) - slope, intercept, r_value, p_value, std_err = stats.linregress(np.array(testx), np.array(testy)) + slope, intercept, r_value, p_value, std_err = stats.linregress( + np.array(testx), np.array(testy) + ) for g in genes_array[gene_test_idx]: if g in networkscores: if run_test_mode == True: - fp.write("%s\t%f\t%f\n" % (g, networkscores[g], slope * networkscores[g] + intercept)) + fp.write( + "%s\t%f\t%f\n" + % ( + g, + networkscores[g], + slope * networkscores[g] + intercept, + ) + ) nbf = slope * networkscores[g] + intercept else: nbf = 0.0 @@ -1003,7 +1108,7 @@ def calculate_bayes_factors( if rna_level == True: fout.write("RNA\tGENE") for i in range(len(column_list)): - fout.write("\t{0:s}".format(column_labels[i])) + fout.write(f"\t{column_labels[i]:s}") if train_method == 0: fout.write("\t{0:s}".format(column_labels[i] + "_STD")) fout.write("\tBF") @@ -1013,28 +1118,30 @@ def calculate_bayes_factors( for rnatag in sorted(bf.keys()): # RNA tag - fout.write("{0:s}\t".format(rnatag)) + fout.write(f"{rnatag:s}\t") # Gene gene = rna2gene[rnatag] - fout.write("{0:s}\t".format(gene)) + fout.write(f"{gene:s}\t") # BF of replicates for rep in column_list: - fout.write("{0:4.3f}\t".format(bf_mean_rna_rep[rnatag][rep])) + fout.write(f"{bf_mean_rna_rep[rnatag][rep]:4.3f}\t") if train_method == 0: - fout.write("{0:4.3f}\t".format(bf_std_rna_rep[rnatag][rep])) + fout.write(f"{bf_std_rna_rep[rnatag][rep]:4.3f}\t") # Sum BF of replicates if filter_multi_target == True: fout.write( - "{0:4.3f}".format(float(bf_multi_corrected_rna[rnatag]) * eqf) + f"{float(bf_multi_corrected_rna[rnatag]) * eqf:4.3f}" ) # eqf = equalizing factor for the number of replicates else: - fout.write("{0:4.3f}".format(float(sum(list(bf_mean_rna_rep[rnatag].values()))) * eqf)) + fout.write( + f"{float(sum(list(bf_mean_rna_rep[rnatag].values()))) * eqf:4.3f}" + ) # Num obs if train_method == 0: - fout.write("\t{0:d}".format(num_obs[gene])) + fout.write(f"\t{num_obs[gene]:d}") fout.write("\n") else: fout.write("GENE") @@ -1051,27 +1158,27 @@ def calculate_bayes_factors( for g in sorted(genes.keys()): # Gene - fout.write("{0:s}".format(g)) + fout.write(f"{g:s}") if network_boost == True: boostedbf_mean = np.mean(boostedbf[g]) boostedbf_std = np.std(boostedbf[g]) - fout.write("\t{0:4.3f}".format(float(boostedbf_mean) * eqf)) + fout.write(f"\t{float(boostedbf_mean) * eqf:4.3f}") if train_method == 0: - fout.write("\t{0:4.3f}".format(float(boostedbf_std) * eqf)) + fout.write(f"\t{float(boostedbf_std) * eqf:4.3f}") # BF if filter_multi_target == True: fout.write( - "\t{0:4.3f}".format(float(bf_multi_corrected_gene[g]) * eqf) + f"\t{float(bf_multi_corrected_gene[g]) * eqf:4.3f}" ) # eqf = equalizing factor for the number of replicates else: - fout.write("\t{0:4.3f}".format(float(bf_mean[g]) * eqf)) + fout.write(f"\t{float(bf_mean[g]) * eqf:4.3f}") # STD, Count if train_method == 0: - fout.write("\t{0:4.3f}\t{1:d}".format(float(bf_std[g]), num_obs[g])) + fout.write(f"\t{float(bf_std[g]):4.3f}\t{num_obs[g]:d}") # Normalized BF if flat_sgrna == True: - fout.write("\t{0:4.3f}".format(float(bf_norm[g]))) + fout.write(f"\t{float(bf_norm[g]):4.3f}") fout.write("\n") @@ -1080,9 +1187,13 @@ def calculate_bayes_factors( @click.option("-i", "--bayes-factors", required=True, type=click.Path(exists=True)) @click.option("-o", "--output-file", required=True) @click.option("-e", "--essential-genes", required=True, type=click.Path(exists=True)) -@click.option("-n", "--non-essential-genes", required=True, type=click.Path(exists=True)) +@click.option( + "-n", "--non-essential-genes", required=True, type=click.Path(exists=True) +) @click.option("-k", "--use-column", default=None) -def calculate_precision_recall(bayes_factors, output_file, essential_genes, non_essential_genes, use_column): +def calculate_precision_recall( + bayes_factors, output_file, essential_genes, non_essential_genes, use_column +): """ Calculate precision-recall from an input Bayes Factors file: @@ -1102,7 +1213,7 @@ def calculate_precision_recall(bayes_factors, output_file, essential_genes, non_ \b Example: - BAGEL.py pr -i input.bf -o output.PR -e ref_essentials.txt -n ref_nonessentials.txt + BAGEL.py pr -i input.bf -o output.PR -e ref_essentials.txt -n ref_nonessentials.txt """ # @@ -1146,9 +1257,7 @@ def calculate_precision_recall(bayes_factors, output_file, essential_genes, non_ if (cumulative_tp > 0) | (cumulative_fp > 0): precision = cumulative_tp / (cumulative_tp + cumulative_fp) fout.write( - "{0:s}\t{1:4.3f}\t{2:4.3f}\t{3:4.3f}\t{4:4.3f}\n".format( - g, bf.loc[g, bf_column], recall, precision, 1.0 - precision - ) + f"{g:s}\t{bf.loc[g, bf_column]:4.3f}\t{recall:4.3f}\t{precision:4.3f}\t{1.0 - precision:4.3f}\n" ) diff --git a/bin/extract_umis.py b/bin/extract_umis.py index e4792d0f..95a186d7 100755 --- a/bin/extract_umis.py +++ b/bin/extract_umis.py @@ -36,7 +36,9 @@ def parse_args(argv): :type argv: List """ usage = "Command line interface to telemap" - parser = argparse.ArgumentParser(description=usage, formatter_class=argparse.RawDescriptionHelpFormatter) + parser = argparse.ArgumentParser( + description=usage, formatter_class=argparse.RawDescriptionHelpFormatter + ) parser.add_argument( "-l", "--log", @@ -78,8 +80,12 @@ def parse_args(argv): default=1, help="Number of threads.", ) - parser.add_argument("--tsv", dest="TSV", type=str, required=False, help="TSV output file") - parser.add_argument("-o", "--output", dest="OUT", type=str, required=False, help="FASTA output file") + parser.add_argument( + "--tsv", dest="TSV", type=str, required=False, help="TSV output file" + ) + parser.add_argument( + "-o", "--output", dest="OUT", type=str, required=False, help="FASTA output file" + ) parser.add_argument( "--fwd-context", dest="FWD_CONTEXT", @@ -108,7 +114,9 @@ def parse_args(argv): default="AAABBBBAABBBBAABBBBAABBBBAAA", help="Reverse UMI sequence", ) - parser.add_argument("INPUT_FA", type=str, nargs="+", default="/dev/stdin", help="Detected UMIs") + parser.add_argument( + "INPUT_FA", type=str, nargs="+", default="/dev/stdin", help="Detected UMIs" + ) args = parser.parse_args(argv) @@ -271,17 +279,25 @@ def extract_umis( for entry in fh: pbar.update(1) - read_5p_seq, read_3p_seq = extract_adapters(entry, max_adapter_length) + read_5p_seq, read_3p_seq = extract_adapters( + entry, max_adapter_length + ) if not read_5p_seq or not read_3p_seq: continue - strand = detect_read_strand(read_5p_seq, upstream_context_fwd, upstream_context_rev) + strand = detect_read_strand( + read_5p_seq, upstream_context_fwd, upstream_context_rev + ) strand_stats[strand] += 1 # Extract fwd UMI - result_5p_fwd_dist, result_5p_fwd_seq = align(read_5p_seq, pattern_fwd, max_pattern_dist) + result_5p_fwd_dist, result_5p_fwd_seq = align( + read_5p_seq, pattern_fwd, max_pattern_dist + ) # Extract rev UMI - result_3p_rev_dist, result_3p_rev_seq = align(read_3p_seq, pattern_rev, max_pattern_dist) + result_3p_rev_dist, result_3p_rev_seq = align( + read_3p_seq, pattern_rev, max_pattern_dist + ) if not result_5p_fwd_seq or not result_3p_rev_seq: continue @@ -302,11 +318,17 @@ def extract_umis( if strand_stats["-"]: fwd_rev_ratio = strand_stats["+"] / strand_stats["-"] logging.info( - "Found {} fwd and {} rev reads (ratio: {})".format(strand_stats["+"], strand_stats["-"], fwd_rev_ratio) + "Found {} fwd and {} rev reads (ratio: {})".format( + strand_stats["+"], strand_stats["-"], fwd_rev_ratio + ) ) if n_read: perc = 100.0 * n_both_umi / n_read - logging.info("{}% of reads contained both UMIs with max {} mismatches".format(perc, max_pattern_dist)) + logging.info( + "{}% of reads contained both UMIs with max {} mismatches".format( + perc, max_pattern_dist + ) + ) if tsv: print( output_file, diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py index e55b8d43..7ee62bfe 100755 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py @@ -59,7 +59,9 @@ def main(): } with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module + versions_by_process = ( + yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module + ) # aggregate versions by the module name (derived from fully-qualified process name) versions_by_module = {} diff --git a/templates/alignment_summary.py b/templates/alignment_summary.py index 035e7b90..1af7a9f6 100644 --- a/templates/alignment_summary.py +++ b/templates/alignment_summary.py @@ -24,10 +24,14 @@ if "aligned-reads" not in line: output_file.write(line) else: - output_file.write(f"aligned-reads, {mapped_reads_count} ({round(mapped_reads_percentage, 1)}%)\\n") + output_file.write( + f"aligned-reads, {mapped_reads_count} ({round(mapped_reads_percentage, 1)}%)\\n" + ) add_line = False if add_line: - output_file.write(f"aligned-reads, {mapped_reads_count} ({round(mapped_reads_percentage, 1)}%)\\n") + output_file.write( + f"aligned-reads, {mapped_reads_count} ({round(mapped_reads_percentage, 1)}%)\\n" + ) with open("versions.yml", "w") as f: f.write('"${task.process}":\\n') diff --git a/templates/preprocessing_summary.py b/templates/preprocessing_summary.py index 13c698ce..122733d1 100755 --- a/templates/preprocessing_summary.py +++ b/templates/preprocessing_summary.py @@ -18,7 +18,9 @@ assembled_reads_count = 0 else: with gzip.open("$assembled_reads", "rt") as handle: - assembled_reads_count = len(list(SeqIO.parse(handle, "fastq"))) # Merged reads R1+R2 + assembled_reads_count = len( + list(SeqIO.parse(handle, "fastq")) + ) # Merged reads R1+R2 with gzip.open("$trimmed_reads", "rt") as handle: trimmed_reads_count = len(list(SeqIO.parse(handle, "fastq"))) # Filtered reads @@ -34,7 +36,9 @@ if field.isdigit(): adapters_count = field # reads with adapters if "%" in field: - adapters_percentage = field # percentage of reads with adapters: ex. "(100.0%)" + adapters_percentage = ( + field # percentage of reads with adapters: ex. "(100.0%)" + ) if "$task.ext.prefix" != "null": prefix = "$task.ext.prefix" From 0c2a01774b0ef3f4503e48e2e9b57c4ebfce921b Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Tue, 13 Feb 2024 09:03:22 +0000 Subject: [PATCH 050/226] fix editorconfig padding --- docs/usage.md | 58 +++++++++++++++++++++++++-------------------------- 1 file changed, 29 insertions(+), 29 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index e95bbe73..ef535a89 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -197,35 +197,35 @@ The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementatio 2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags) 3. Create the custom config accordingly: - - For Docker: - - ```nextflow - process { - withName: PANGOLIN { - container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0' - } - } - ``` - - - For Singularity: - - ```nextflow - process { - withName: PANGOLIN { - container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0' - } - } - ``` - - - For Conda: - - ```nextflow - process { - withName: PANGOLIN { - conda = 'bioconda::pangolin=3.0.5' - } - } - ``` +- For Docker: + + ```nextflow + process { + withName: PANGOLIN { + container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0' + } + } + ``` + +- For Singularity: + + ```nextflow + process { + withName: PANGOLIN { + container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0' + } + } + ``` + +- For Conda: + + ```nextflow + process { + withName: PANGOLIN { + conda = 'bioconda::pangolin=3.0.5' + } + } + ``` :::info If you wish to periodically update individual tool-specific results (e.g. Pangolin) generated by the pipeline then you must ensure to keep the `work/` directory otherwise the `-resume` ability of the pipeline will be compromised and it will restart from scratch. From 021416180724956d59903d5391cd17907c4eadea Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Tue, 13 Feb 2024 16:51:26 +0100 Subject: [PATCH 051/226] fix syntax error with elvis operator and lists --- workflows/crisprseq_screening.nf | 8 +++++++- workflows/crisprseq_targeted.nf | 7 ++++++- 2 files changed, 13 insertions(+), 2 deletions(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 8eb56572..fbaa87ea 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -97,7 +97,13 @@ workflow CRISPRSEQ_SCREENING { Channel.fromSamplesheet("input") .map{ meta, fastq_1, fastq_2, x, y, z -> // x (reference), y (protospacer), and z (template) are part of the targeted workflows and we don't need them - return [ meta + [ single_end:fastq_2?false:true ], fastq_2?[ fastq_1, fastq_2 ]:[ fastq_1 ] ] } + if (fastq_2) { + files = [ fastq_1, fastq_2 ] + } else { + files = [ fastq_1 ] + } + return [ meta + [ single_end:fastq_2?false:true ], files ] + } .set { ch_input } diff --git a/workflows/crisprseq_targeted.nf b/workflows/crisprseq_targeted.nf index abf67bb2..4138470f 100644 --- a/workflows/crisprseq_targeted.nf +++ b/workflows/crisprseq_targeted.nf @@ -140,7 +140,12 @@ workflow CRISPRSEQ_TARGETED { Channel.fromSamplesheet("input") .multiMap { meta, fastq_1, fastq_2, reference, protospacer, template -> // meta.condition is part of the screening workflow and we need to remove it - reads: [ meta.id, meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], fastq_2?[ fastq_1, fastq_2 ]:[ fastq_1 ] ] + if (fastq_2) { + files = [ fastq_1, fastq_2 ] + } else { + files = [ fastq_1 ] + } + reads: [ meta.id, meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], files ] reference: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], reference] protospacer: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], protospacer] template: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], template] From c4e9954d99fb8735431de714bf183df6589c3de4 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Wed, 14 Feb 2024 10:43:41 +0100 Subject: [PATCH 052/226] Update workflows/crisprseq_screening.nf MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Júlia Mir Pedrol --- workflows/crisprseq_screening.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 12d80d2a..0f9789d3 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -19,7 +19,7 @@ WorkflowCrisprseq.initialise(params, log) if (params.library) { ch_library = file(params.library) } if (params.crisprcleanr) { if(params.crisprcleanr.endsWith(".csv")) { - ch_crisprcleanr_file = file(params.crisprcleanr) + ch_crisprcleanr_file = Channel.fromPath(params.crisprcleanr) } else { ch_crisprcleanr_value = Channel.value(params.crisprcleanr) } From d2053cdb2caa65bbc814b9642a259ca34cbaa2be Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Thu, 15 Feb 2024 16:05:28 +0100 Subject: [PATCH 053/226] Add mageckFlute --- conf/modules.config | 4 ++ modules/local/mageck/flutemle.nf | 58 ++++++++++++++++++++++ modules/nf-core/mageck/mle/mageck-mle.diff | 2 +- modules/nf-core/mageck/mle/main.nf | 2 +- workflows/crisprseq_screening.nf | 4 ++ 5 files changed, 68 insertions(+), 2 deletions(-) create mode 100644 modules/local/mageck/flutemle.nf diff --git a/conf/modules.config b/conf/modules.config index 2c6330b3..354ff8bf 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -126,6 +126,10 @@ process { containerOptions = '' } + withName: MAGECK_FLUTEMLE { + containerOptions = '' + } + withName: CRISPRCLEANR_NORMALIZE { publishDir = [ path: { "${params.outdir}/crisprcleanr/normalization/" }, diff --git a/modules/local/mageck/flutemle.nf b/modules/local/mageck/flutemle.nf new file mode 100644 index 00000000..efe8ac9e --- /dev/null +++ b/modules/local/mageck/flutemle.nf @@ -0,0 +1,58 @@ + +process MAGECK_FLUTEMLE { + tag "$prefix" + label 'process_high' + + conda "bioconda::bioconductor-mageckflute=2.2.0" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/mageckflute:2.2.0--r42hdfd78af_0': + 'biocontainers/bioconductor-mageckflute:2.2.0--r42hdfd78af_0' }" + + input: + tuple val(meta), path(gene_summary) + + output: + tuple val(meta), path("MAGeCKFlute_*/Enrichment/*"), emit: enrich + tuple val(meta), path("MAGeCKFlute_*/QC/*"), emit: qc + tuple val(meta), path("MAGeCKFlute_*/Selection/*"), emit: select + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + prefix = meta.id ?: "${meta.treatment}_vs_${meta.reference}" + + """ + #!/usr/bin/env Rscript + #### author: Laurence Kuhlburger + #### Released under the MIT license. See git repository (https://github.com/nf-core/crisprseq) for full license text. + #### + #### graphs mageck MLE + + library(MAGeCKFlute) + library(clusterProfiler) + library(ggplot2) + + #library(pathview) + options(ggrepel.max.overlaps = Inf) + mle <- read.table("${gene_summary}", header = TRUE, sep = "\t", stringsAsFactors = FALSE) + print(head(mle)) + colnames(mle) + #stop(colnames(mle)) + FluteMLE(mle, treatname= "${prefix}", proj="${prefix}", pathview.top=0, $args) + + version_file_path <- "versions.yml" + version_flute <- paste(unlist(packageVersion("MAGeCKFlute")), collapse = ".") + version_ggplot <- paste(unlist(packageVersion("ggplot2")), collapse = ".") + + f <- file(version_file_path, "w") + writeLines('"${task.process}":', f) + writeLines(" MAGeCKFlute: ", f, sep = "") + writeLines(version_flute, f) + writeLines(" ggplot2: ", f, sep = "") + writeLines(version_ggplot, f) + close(f) + """ +} diff --git a/modules/nf-core/mageck/mle/mageck-mle.diff b/modules/nf-core/mageck/mle/mageck-mle.diff index c830b3fe..e7b6f9ec 100644 --- a/modules/nf-core/mageck/mle/mageck-mle.diff +++ b/modules/nf-core/mageck/mle/mageck-mle.diff @@ -25,7 +25,7 @@ Changes in module 'nf-core/mageck/mle' script: def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" -+ prefix = meta.id ?: "${meta.treatment}_vs_${meta.treatment}" ++ prefix = meta.id ?: "${meta.treatment}_vs_${meta.reference}" + """ diff --git a/modules/nf-core/mageck/mle/main.nf b/modules/nf-core/mageck/mle/main.nf index 4dbe4953..a08a0431 100644 --- a/modules/nf-core/mageck/mle/main.nf +++ b/modules/nf-core/mageck/mle/main.nf @@ -20,7 +20,7 @@ process MAGECK_MLE { script: def args = task.ext.args ?: '' - prefix = meta.id ?: "${meta.treatment}_vs_${meta.treatment}" + prefix = meta.id ?: "${meta.treatment}_vs_${meta.reference}" """ diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index e000a907..f9925709 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -62,6 +62,7 @@ include { BAGEL2_BF } from '../modules/local/bagel2/bf' include { BAGEL2_PR } from '../modules/local/bagel2/pr' include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' include { MATRICESCREATION } from '../modules/local/matricescreation' +include { MAGECK_FLUTEMLE } from '../modules/local/mageck/flutemle' // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules @@ -271,12 +272,15 @@ workflow CRISPRSEQ_SCREENING { }.set { ch_designed_mle } ch_mle = ch_designed_mle.combine(ch_counts) MAGECK_MLE_MATRIX (ch_mle) + MAGECK_FLUTEMLE(MAGECK_MLE.out.gene_summary) + } if(params.contrasts) { MATRICESCREATION(ch_contrasts) ch_mle = MATRICESCREATION.out.design_matrix.combine(ch_counts) MAGECK_MLE (ch_mle) ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) + MAGECK_FLUTEMLE(MAGECK_MLE.out.gene_summary) } } From 437c0dfb48bd5dadba653a1bc09d061e00287907 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Thu, 15 Feb 2024 16:14:05 +0100 Subject: [PATCH 054/226] Change name of crisprcleanr channel --- workflows/crisprseq_screening.nf | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 0f9789d3..a4c4a518 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -19,9 +19,9 @@ WorkflowCrisprseq.initialise(params, log) if (params.library) { ch_library = file(params.library) } if (params.crisprcleanr) { if(params.crisprcleanr.endsWith(".csv")) { - ch_crisprcleanr_file = Channel.fromPath(params.crisprcleanr) + ch_crisprcleanr = Channel.fromPath(params.crisprcleanr) } else { - ch_crisprcleanr_value = Channel.value(params.crisprcleanr) + ch_crisprcleanr = Channel.value(params.crisprcleanr) } } @@ -189,7 +189,7 @@ workflow CRISPRSEQ_SCREENING { CRISPRCLEANR_NORMALIZE( ch_crispr_normalize.collect(), '', - ch_crisprcleanr_file, + ch_crisprcleanr, params.min_reads, params.min_targeted_genes ) } else @@ -197,7 +197,7 @@ workflow CRISPRSEQ_SCREENING { ch_crispr_normalize = Channel.of([id: "count_table_normalize"]).concat(ch_counts) CRISPRCLEANR_NORMALIZE( ch_crispr_normalize.collect(), - ch_crisprcleanr_value, + ch_crisprcleanr, [], params.min_reads, params.min_targeted_genes) From b73a0d4912d1206b61362f41490bdfba91c20c27 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Thu, 15 Feb 2024 16:16:05 +0100 Subject: [PATCH 055/226] run pre commit --- CHANGELOG.md | 1 - docs/usage/screening.md | 16 ++++++++-------- 2 files changed, 8 insertions(+), 9 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 589d56a5..4785e9fa 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -11,7 +11,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Added a csv input option for crisprcleanr ([#105](https://github.com/nf-core/crisprseq/pull/105)) - Added a contrasts parameter so the pipeline automatically creates design matrices and MAGeCK MLE ([#109](https://github.com/nf-core/crisprseq/pull/109)) - ## [v2.1.1 - Jamon Salas - patch](https://github.com/nf-core/crisprseq/releases/tag/2.1.1) - [14.12.2023] ### Added diff --git a/docs/usage/screening.md b/docs/usage/screening.md index a455a871..ba7fcc47 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -62,13 +62,13 @@ CRISPRcleanR is used for gene count normalization and the removal of biases for Most used library already have an annotation dataset which you can find [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). Otherwise, if you wish to provide your own file, please provide it in csv form, and make sure it follows the following format : -| ,CODE | GENES | EXONE | CHRM | STRAND | STARTpos | ENDpos | -|----------------------|-------------------|-------|------|--------|-----------|-----------| -| AAAAAAAAAAAATGCATTCT | NM_183035.1 | Defb34| ex2 | 8 | - | 19126349 | 19126369 | -| AAAAAAAAATAAGCTCACCC | NM_001170853.1 | Mndal | ex5 | 1 | + | 173872968 | 173872988 | -| AAAAAAAATCCTGTCGCCCA | NM_001039049.1 | Cox8c | ex1 | 12 | + | 102899487 | 102899507 | -| AAAAAAATCGGCATACCATG | NM_178627.3 | Poldip3| ex4 | 15 | - | 83135295 | 83135315 | -| AAAAAAATGACATTACTGCA | NM_026602.3 | Bcas2 | ex4 | 3 | + | 103174386 | 103174406 | +| ,CODE | GENES | EXONE | CHRM | STRAND | STARTpos | ENDpos | +| -------------------- | -------------- | ------- | ---- | ------ | -------- | --------- | --------- | +| AAAAAAAAAAAATGCATTCT | NM_183035.1 | Defb34 | ex2 | 8 | - | 19126349 | 19126369 | +| AAAAAAAAATAAGCTCACCC | NM_001170853.1 | Mndal | ex5 | 1 | + | 173872968 | 173872988 | +| AAAAAAAATCCTGTCGCCCA | NM_001039049.1 | Cox8c | ex1 | 12 | + | 102899487 | 102899507 | +| AAAAAAATCGGCATACCATG | NM_178627.3 | Poldip3 | ex4 | 15 | - | 83135295 | 83135315 | +| AAAAAAATGACATTACTGCA | NM_026602.3 | Bcas2 | ex4 | 3 | + | 103174386 | 103174406 | ### Running MAGeCK MLE and BAGEL2 with a contrast file @@ -83,7 +83,7 @@ A full example can be found [here](https://raw.githubusercontent.com/nf-core/tes ### Running MAGeCK RRA only -MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section (with a `.txt` extension) and also specify `--rra` . +MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section (with a `.txt` extension) and also specify `--rra` . ### Running MAGeCK MLE only From bd7c1b1e24417e3a3a5afee4294da7ed21761c10 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Thu, 15 Feb 2024 16:38:58 +0100 Subject: [PATCH 056/226] Ran pre commit --- CHANGELOG.md | 1 - docs/usage/screening.md | 16 ++++++++-------- 2 files changed, 8 insertions(+), 9 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 589d56a5..4785e9fa 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -11,7 +11,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Added a csv input option for crisprcleanr ([#105](https://github.com/nf-core/crisprseq/pull/105)) - Added a contrasts parameter so the pipeline automatically creates design matrices and MAGeCK MLE ([#109](https://github.com/nf-core/crisprseq/pull/109)) - ## [v2.1.1 - Jamon Salas - patch](https://github.com/nf-core/crisprseq/releases/tag/2.1.1) - [14.12.2023] ### Added diff --git a/docs/usage/screening.md b/docs/usage/screening.md index a455a871..ba7fcc47 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -62,13 +62,13 @@ CRISPRcleanR is used for gene count normalization and the removal of biases for Most used library already have an annotation dataset which you can find [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). Otherwise, if you wish to provide your own file, please provide it in csv form, and make sure it follows the following format : -| ,CODE | GENES | EXONE | CHRM | STRAND | STARTpos | ENDpos | -|----------------------|-------------------|-------|------|--------|-----------|-----------| -| AAAAAAAAAAAATGCATTCT | NM_183035.1 | Defb34| ex2 | 8 | - | 19126349 | 19126369 | -| AAAAAAAAATAAGCTCACCC | NM_001170853.1 | Mndal | ex5 | 1 | + | 173872968 | 173872988 | -| AAAAAAAATCCTGTCGCCCA | NM_001039049.1 | Cox8c | ex1 | 12 | + | 102899487 | 102899507 | -| AAAAAAATCGGCATACCATG | NM_178627.3 | Poldip3| ex4 | 15 | - | 83135295 | 83135315 | -| AAAAAAATGACATTACTGCA | NM_026602.3 | Bcas2 | ex4 | 3 | + | 103174386 | 103174406 | +| ,CODE | GENES | EXONE | CHRM | STRAND | STARTpos | ENDpos | +| -------------------- | -------------- | ------- | ---- | ------ | -------- | --------- | --------- | +| AAAAAAAAAAAATGCATTCT | NM_183035.1 | Defb34 | ex2 | 8 | - | 19126349 | 19126369 | +| AAAAAAAAATAAGCTCACCC | NM_001170853.1 | Mndal | ex5 | 1 | + | 173872968 | 173872988 | +| AAAAAAAATCCTGTCGCCCA | NM_001039049.1 | Cox8c | ex1 | 12 | + | 102899487 | 102899507 | +| AAAAAAATCGGCATACCATG | NM_178627.3 | Poldip3 | ex4 | 15 | - | 83135295 | 83135315 | +| AAAAAAATGACATTACTGCA | NM_026602.3 | Bcas2 | ex4 | 3 | + | 103174386 | 103174406 | ### Running MAGeCK MLE and BAGEL2 with a contrast file @@ -83,7 +83,7 @@ A full example can be found [here](https://raw.githubusercontent.com/nf-core/tes ### Running MAGeCK RRA only -MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section (with a `.txt` extension) and also specify `--rra` . +MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section (with a `.txt` extension) and also specify `--rra` . ### Running MAGeCK MLE only From 0fd221ca6ac6ba75c7966cd0b62339de07ba8086 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 19 Feb 2024 16:32:10 +0100 Subject: [PATCH 057/226] Add 3' and 5' for cutadapt --- conf/modules.config | 7 ++- nextflow.config | 4 +- nextflow_schema.json | 46 +++++++++--------- workflows/crisprseq_screening.nf | 80 +++++++++++++++++--------------- 4 files changed, 73 insertions(+), 64 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 2c6330b3..03c310ae 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -137,8 +137,11 @@ process { if(params.analysis == 'targeted') { ext.args = '-g file:overrepresented.fasta -N' } - if(params.analysis == 'screening' && params.cutadapt) { - ext.args = "-a ${params.cutadapt}" + if(params.analysis == 'screening' && params.three_prime_adapter) { + ext.args = "-a ${params.three_prime_adapter}" + } + if(params.analysis == 'screening' && params.five_prime_adapter) { + ext.args = "-g ${params.five_prime_adapter}" } publishDir = [ path: { "${params.outdir}/preprocessing/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, diff --git a/nextflow.config b/nextflow.config index 96c19bb8..4ca1a12e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -17,9 +17,11 @@ params { library = null crisprcleanr = null contrasts = null - cutadapt = null mle_design_matrix = null count_table = null + fasta = null + five_prime_adapter = null + three_prime_adapter = null min_reads = 30 min_targeted_genes = 3 rra = false diff --git a/nextflow_schema.json b/nextflow_schema.json index ee6eafb1..06dada55 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -150,6 +150,25 @@ "description": "Parameters used for functional genomic screenings", "default": "", "properties": { + "library": { + "type": "string", + "format": "file-path", + "pattern": "^\\S+\\.(tsv|txt)$", + "mimetype": "text/tsv", + "exists": true, + "fa_icon": "far fa-address-book", + "description": "sgRNA and targetting genes, tab separated" + }, + "five_prime_adapter": { + "type": "string" + }, + "three_prime_adapter": { + "type": "string", + "description": "Sequencing adapter sequence to use for trimming." + }, + "fasta": { + "type": "string" + }, "mle_design_matrix": { "type": "string", "format": "file-path", @@ -164,8 +183,7 @@ }, "rra": { "type": "boolean", - "description": "Parameter indicating if MAGeCK RRA should be ran instead of MAGeCK MLE.", - "default": false + "description": "Parameter indicating if MAGeCK RRA should be ran instead of MAGeCK MLE." }, "count_table": { "type": "string", @@ -175,32 +193,19 @@ "exists": true, "description": "Please provide your count table if the mageck test should be skipped." }, - "library": { - "type": "string", - "format": "file-path", - "pattern": "^\\S+\\.(tsv|txt)$", - "mimetype": "text/tsv", - "exists": true, - "fa_icon": "far fa-address-book", - "description": "sgRNA and targetting genes, tab separated" - }, "crisprcleanr": { "type": "string", "description": "sgRNA library annotation for crisprcleanR" }, - "cutadapt": { - "type": "string", - "description": "cut adapter for screening analysis" - }, "min_reads": { "type": "number", "description": "a filter threshold value for sgRNAs, based on their average counts in the control sample", - "default": 30 + "default": 30.0 }, "min_targeted_genes": { "type": "number", "description": "Minimal number of different genes targeted by sgRNAs in a biased segment in order for the corresponding counts to be corrected for CRISPRcleanR", - "default": 3 + "default": 3.0 }, "bagel_reference_essentials": { "type": "string", @@ -482,10 +487,5 @@ { "$ref": "#/definitions/generic_options" } - ], - "properties": { - "fasta": { - "type": "string" - } - } + ] } diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 298d36ac..0c56af2d 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -34,8 +34,12 @@ if(params.rra && params.mle_design_matrix) { warning "mle_design_matrix will only be used for the MAGeCK MLE computations" } -if(params.rra && !params.contrasts) { - error "Please also provide the contrasts table to compare the samples for MAGeCK RRA" +if(params.fasta && params.count_table) { + error "Please provide either a fasta file or a count_table" + } + +if(params.fasta && !params.library) { + error "Please provide a fasta file and the library file" } if(params.rra && params.mle_design_matrix) { @@ -85,7 +89,8 @@ include { MATRICESCREATION } from '../modules/local/matricescreation' // MODULE: Installed directly from nf-core/modules // include { FASTQC } from '../modules/nf-core/fastqc/main' -include { CUTADAPT } from '../modules/nf-core/cutadapt/main' +include { CUTADAPT as CUTADAPT_THREE_PRIME } from '../modules/nf-core/cutadapt/main' +include { CUTADAPT as CUTADAPT_FIVE_PRIME } from '../modules/nf-core/cutadapt/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' include { MAGECK_COUNT } from '../modules/nf-core/mageck/count/main' include { MAGECK_MLE } from '../modules/nf-core/mageck/mle/main' @@ -94,8 +99,8 @@ include { MAGECK_GRAPHRRA } from '../modules/local/mageck/grap include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' include { CRISPRCLEANR_NORMALIZE } from '../modules/nf-core/crisprcleanr/normalize/main' include { MAGECK_MLE as MAGECK_MLE_MATRIX } from '../modules/nf-core/mageck/mle/main' -include { BOWTIE2_BUILD } from '../modules/nf-core/bowtie2/build/main' -include { BOWTIE2_ALIGN } from '../modules/nf-core/bowtie2/align/main' +include { BOWTIE2_BUILD } from '../modules/nf-core/bowtie2/build/main' +include { BOWTIE2_ALIGN } from '../modules/nf-core/bowtie2/align/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -111,23 +116,6 @@ workflow CRISPRSEQ_SCREENING { ch_versions = Channel.empty() if(!params.count_table){ - // - // If bowtie aligner is used, convert the library in text format to a fasta file - // - if (params.library) { - ch_library = Channel.fromPath(params.library) - ch_library.map { line -> - fasta_line = text_to_fasta(line) - fasta_line - }.collectFile(name: 'library.fa', newLine: true).set{ ch_fasta } - } - ch_text_to_fasta = Channel.of([id: "text to fasta"]) - if(params.fasta){ ch_fasta = Channel.fromPath(params.fasta) } - - // create bowtie index - BOWTIE2_BUILD(ch_text_to_fasta.concat(ch_fasta).collect()) - //ch_versions = ch_versions.mix(BOWTIE2_BUILD.out.versions) - // // Create input channel from input file provided through params.input // @@ -142,28 +130,44 @@ workflow CRISPRSEQ_SCREENING { return [ meta + [ single_end:fastq_2?false:true ], files ] } .set { ch_input } - ch_input.dump(tag: "ch_input") - BOWTIE2_ALIGN ( - ch_input, - BOWTIE2_BUILD.out.index, - false, - false - ) + //set adapter seq to null to make it compatible with crispr targeted + ch_cutadapt = ch_input.combine(Channel.value([[]])) - ch_input_cutadapt = ch_input.combine(Channel.value([[]])) - - if(params.cutadapt) { - CUTADAPT( + if(params.three_prime_adapter) { + CUTADAPT_THREE_PRIME( ch_input_cutadapt ) - ch_versions = ch_versions.mix(CUTADAPT.out.versions) + ch_cutadapt = CUTADAPT_THREE_PRIME.out.reads + ch_versions = ch_versions.mix(CUTADAPT_THREE_PRIME.out.versions) + } + if(params.five_prime_adapter) { + CUTADAPT_FIVE_PRIME( + ch_cutadapt + ) + ch_cutadapt = CUTADAPT_FIVE_PRIME.out.reads + ch_versions = ch_versions.mix(CUTADAPT.out.versions) + } - CUTADAPT.out.reads - .map{ meta, fastq -> - [meta, [fastq]] + if(params.five_prime_adapter || params.three_prime_adapter) { + ch_cutadapt + .map{ meta, fastq -> + [meta, [fastq]] + } + .set { ch_input } } - .set { ch_input } + + if(params.fasta){ + ch_fasta = Channel.fromPath(params.fasta) + BOWTIE2_BUILD(ch_fasta) + ch_versions = ch_versions.mix(BOWTIE2_BUILD.out.versions) + + BOWTIE2_ALIGN ( + ch_input, + BOWTIE2_BUILD.out.index, + false, + false + ) } // From 23031ff21d9ef1c46f15260d8002809363a4e431 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 20 Feb 2024 15:28:05 +0000 Subject: [PATCH 058/226] Template update for nf-core/tools version 2.13 --- .editorconfig | 9 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/branch.yml | 2 +- .github/workflows/ci.yml | 7 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 17 +- .github/workflows/linting.yml | 12 +- .github/workflows/linting_comment.yml | 4 +- .github/workflows/release-announcements.yml | 11 +- README.md | 5 +- assets/multiqc_config.yml | 2 + assets/schema_input.json | 21 +- bin/check_samplesheet.py | 259 ----------- conf/modules.config | 8 - lib/NfcoreTemplate.groovy | 356 -------------- lib/Utils.groovy | 47 -- lib/WorkflowCrisprseq.groovy | 122 ----- lib/WorkflowMain.groovy | 77 --- main.nf | 100 ++-- modules.json | 28 +- modules/local/samplesheet_check.nf | 31 -- .../dumpsoftwareversions/environment.yml | 7 - .../custom/dumpsoftwareversions/main.nf | 24 - .../custom/dumpsoftwareversions/meta.yml | 37 -- .../templates/dumpsoftwareversions.py | 102 ---- .../dumpsoftwareversions/tests/main.nf.test | 43 -- .../tests/main.nf.test.snap | 33 -- .../dumpsoftwareversions/tests/tags.yml | 2 - modules/nf-core/fastqc/tests/main.nf.test | 14 +- .../nf-core/fastqc/tests/main.nf.test.snap | 76 ++- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- modules/nf-core/multiqc/tests/main.nf.test | 13 +- .../nf-core/multiqc/tests/main.nf.test.snap | 32 +- nextflow.config | 5 +- nextflow_schema.json | 1 + pyproject.toml | 8 +- subworkflows/local/input_check.nf | 44 -- .../utils_nfcore_crisprseq_pipeline/main.nf | 247 ++++++++++ .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 +++ .../tests/main.function.nf.test.snap | 12 + .../tests/main.workflow.nf.test | 123 +++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 440 ++++++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 ++++++ .../tests/main.function.nf.test.snap | 138 ++++++ .../tests/main.workflow.nf.test | 29 ++ .../tests/main.workflow.nf.test.snap | 15 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 +++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 ++++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + workflows/crisprseq.nf | 133 ++---- 61 files changed, 2108 insertions(+), 1406 deletions(-) delete mode 100755 bin/check_samplesheet.py delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100644 lib/Utils.groovy delete mode 100755 lib/WorkflowCrisprseq.groovy delete mode 100755 lib/WorkflowMain.groovy delete mode 100644 modules/local/samplesheet_check.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml delete mode 100644 subworkflows/local/input_check.nf create mode 100644 subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/.editorconfig b/.editorconfig index 9b990088..dd9ffa53 100644 --- a/.editorconfig +++ b/.editorconfig @@ -18,7 +18,12 @@ end_of_line = unset insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset -indent_size = unset +[/subworkflows/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset [/assets/email*] indent_size = unset @@ -28,5 +33,5 @@ indent_size = unset indent_style = unset # ignore python -[*.{py}] +[*.{py,md}] indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index b393817c..82f78840 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index f46f773a..539cd12f 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 29192d3d..856008b0 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v2 + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index fbb59dab..f3fb085a 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,13 +28,16 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 with: version: "${{ matrix.NXF_VER }}" + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: Run pipeline with test data # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index e37cfda5..0b6b1f27 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v9 + - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458a..f823210d 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -6,6 +6,11 @@ name: Test successful pipeline download with 'nf-core download' # - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. on: workflow_dispatch: + inputs: + testbranch: + description: "The specific branch you wish to utilize for the test execution of nf-core download." + required: true + default: "dev" pull_request: types: - opened @@ -23,13 +28,13 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" - - uses: eWaterCycle/setup-singularity@v7 + - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: singularity-version: 3.8.3 @@ -42,13 +47,13 @@ jobs: run: | echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} - echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} - name: Download the pipeline env: NXF_SINGULARITY_CACHEDIR: ./ run: | - nf-core download ${{ env.REPO_LOWERCASE }} \ + nf-core download ${{ env.REPO_LOWERCASE }} \ --revision ${{ env.REPO_BRANCH }} \ --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ --compress "none" \ @@ -64,4 +69,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 81cd098e..748b4311 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,10 +14,10 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Set up Python 3.11 - uses: actions/setup-python@v5 + uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: 3.11 cache: "pip" @@ -32,12 +32,12 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v4 + uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 147bcd10..b706875f 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v3 + uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 with: workflow: linting.yml workflow_conclusion: completed @@ -21,7 +21,7 @@ jobs: run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@v2 + uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 21ac3f06..c3674af2 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -9,6 +9,11 @@ jobs: toot: runs-on: ubuntu-latest steps: + - name: get topics and convert to hashtags + id: get_topics + run: | + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + - uses: rzr/fediverse-action@master with: access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} @@ -20,11 +25,13 @@ jobs: Please see the changelog: ${{ github.event.release.html_url }} + ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics + send-tweet: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +63,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.1.0 + - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/README.md b/README.md index 177d1c85..9e23efde 100644 --- a/README.md +++ b/README.md @@ -4,8 +4,9 @@ nf-core/crisprseq -[![GitHub Actions CI Status](https://github.com/nf-core/crisprseq/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/crisprseq/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/nf-core/crisprseq/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/crisprseq/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/crisprseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) + +[![GitHub Actions CI Status](https://github.com/nf-core/crisprseq/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/crisprseq/actions/workflows/ci.yml) +[![GitHub Actions Linting Status](https://github.com/nf-core/crisprseq/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/crisprseq/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/crisprseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 1e43bf26..e500d031 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -11,3 +11,5 @@ report_section_order: order: -1002 export_plots: true + +disable_version_detection: true diff --git a/assets/schema_input.json b/assets/schema_input.json index 7327787f..957a041f 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,25 +10,22 @@ "sample": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] }, "fastq_1": { "type": "string", + "format": "file-path", + "exists": true, "pattern": "^\\S+\\.f(ast)?q\\.gz$", "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" }, "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" } }, "required": ["sample", "fastq_1"] diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index 4a758fe0..00000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,259 +0,0 @@ -#!/usr/bin/env python - - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging -import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ) - - def __init__( - self, - sample_col="sample", - first_col="fastq_1", - second_col="fastq_2", - single_col="single_end", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - first_col (str): The name of the column that contains the first (or only) - FASTQ file path (default "fastq_1"). - second_col (str): The name of the column that contains the second (if any) - FASTQ file path (default "fastq_2"). - single_col (str): The name of the new column that will be inserted and - records whether the sample contains single- or paired-end sequencing - reads (default "single_end"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._first_col = first_col - self._second_col = second_col - self._single_col = single_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_first(row) - self._validate_second(row) - self._validate_pair(row) - self._seen.add((row[self._sample_col], row[self._first_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_first(self, row): - """Assert that the first FASTQ entry is non-empty and has the right format.""" - if len(row[self._first_col]) <= 0: - raise AssertionError("At least the first FASTQ file is required.") - self._validate_fastq_format(row[self._first_col]) - - def _validate_second(self, row): - """Assert that the second FASTQ entry has the right format if it exists.""" - if len(row[self._second_col]) > 0: - self._validate_fastq_format(row[self._second_col]) - - def _validate_pair(self, row): - """Assert that read pairs have the same file extension. Report pair status.""" - if row[self._first_col] and row[self._second_col]: - row[self._single_col] = False - first_col_suffix = Path(row[self._first_col]).suffixes[-2:] - second_col_suffix = Path(row[self._second_col]).suffixes[-2:] - if first_col_suffix != second_col_suffix: - raise AssertionError("FASTQ pairs must have the same file extensions.") - else: - row[self._single_col] = True - - def _validate_fastq_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The FASTQ file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. - - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" - - -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) - - -def sniff_format(handle): - """ - Detect the tabular format. - - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - dialect = sniffer.sniff(peek) - return dialect - - -def check_samplesheet(file_in, file_out): - """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. Also add - an additional column which records whether one or two FASTQ reads were found. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - - """ - required_columns = {"sample", "fastq_1", "fastq_2"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") - sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - header.insert(1, "single_end") - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) - - -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - return parser.parse_args(argv) - - -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/conf/modules.config b/conf/modules.config index d91c6aba..e3ea8fa6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,14 +18,6 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: SAMPLESHEET_CHECK { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: FASTQC { ext.args = '--quiet' } diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index e248e4c3..00000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,356 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") - sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); - output_hf.delete() - - // Write summary e-mail TXT to a file - def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); - output_tf.delete() - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Dump pipeline parameters in a json file - // - public static void dump_parameters(workflow, params) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) - - FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") - temp_pf.delete() - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100644 index 8d030f4e..00000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowCrisprseq.groovy b/lib/WorkflowCrisprseq.groovy deleted file mode 100755 index 9fdb71d2..00000000 --- a/lib/WorkflowCrisprseq.groovy +++ /dev/null @@ -1,122 +0,0 @@ -// -// This file holds several functions specific to the workflow/crisprseq.nf in the nf-core/crisprseq pipeline -// - -import nextflow.Nextflow -import groovy.text.SimpleTemplateEngine - -class WorkflowCrisprseq { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - - genomeExistsError(params, log) - - - if (!params.fasta) { - Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - } - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

$group

\n" - summary_section += "
\n" - for (param in group_params.keySet()) { - summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" - } - summary_section += "
\n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Generate methods description for MultiQC - // - - public static String toolCitationText(params) { - - // TODO nf-core: Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text - } - - public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text - } - - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file - def meta = [:] - meta.workflow = run_workflow.toMap() - meta["manifest_map"] = run_workflow.manifest.toMap() - - // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " - - // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) - - - def methods_text = mqc_methods_yaml.text - - def engine = new SimpleTemplateEngine() - def description_html = engine.createTemplate(methods_text).make(meta) - - return description_html - } - - // - // Exit pipeline if incorrect --genome key provided - // - private static void genomeExistsError(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - Nextflow.error(error_string) - } - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index 46389206..00000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,77 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the nf-core/crisprseq pipeline -// - -import nextflow.Nextflow - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log, args) { - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - // Check that the profile doesn't contain spaces and doesn't end with a trailing comma - checkProfile(workflow.profile, args, log) - - // Check that conda channels are set-up correctly - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") - } - } - // - // Get attribute from genome config file e.g. fasta - // - public static Object getGenomeAttribute(params, attribute) { - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - return params.genomes[ params.genome ][ attribute ] - } - } - return null - } - - // - // Exit pipeline if --profile contains spaces - // - private static void checkProfile(profile, args, log) { - if (profile.endsWith(',')) { - Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - if (args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - } -} diff --git a/main.nf b/main.nf index 1219869f..b682cefa 100644 --- a/main.nf +++ b/main.nf @@ -13,66 +13,96 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// TODO nf-core: Remove this line if you don't need a FASTA file -// This is an example of how to use getGenomeAttribute() to fetch parameters -// from igenomes.config using `--genome` -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +include { CRISPRSEQ } from './workflows/crisprseq' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_crisprseq_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_crisprseq_pipeline' + +include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_crisprseq_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE & PRINT PARAMETER SUMMARY + GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { validateParameters; paramsHelp } from 'plugin/nf-validation' - -// Print help message if needed -if (params.help) { - def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) - def citation = '\n' + WorkflowMain.citation(workflow) + '\n' - def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" - log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) - System.exit(0) -} - -// Validate input parameters -if (params.validate_params) { - validateParameters() -} - -WorkflowMain.initialise(workflow, params, log, args) +// TODO nf-core: Remove this line if you don't need a FASTA file +// This is an example of how to use getGenomeAttribute() to fetch parameters +// from igenomes.config using `--genome` +params.fasta = getGenomeAttribute('fasta') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - NAMED WORKFLOW FOR PIPELINE + NAMED WORKFLOWS FOR PIPELINE ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { CRISPRSEQ } from './workflows/crisprseq' - // -// WORKFLOW: Run main nf-core/crisprseq analysis pipeline +// WORKFLOW: Run main analysis pipeline depending on type of input // workflow NFCORE_CRISPRSEQ { - CRISPRSEQ () -} + take: + samplesheet // channel: samplesheet read in from --input + + main: + + // + // WORKFLOW: Run pipeline + // + CRISPRSEQ ( + samplesheet + ) + + emit: + multiqc_report = CRISPRSEQ.out.multiqc_report // channel: /path/to/multiqc_report.html + +} /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN ALL WORKFLOWS + RUN MAIN WORKFLOW ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// WORKFLOW: Execute a single named workflow for the pipeline -// See: https://github.com/nf-core/rnaseq/issues/619 -// workflow { - NFCORE_CRISPRSEQ () + + main: + + // + // SUBWORKFLOW: Run initialisation tasks + // + PIPELINE_INITIALISATION ( + params.version, + params.help, + params.validate_params, + params.monochrome_logs, + args, + params.outdir, + params.input + ) + + // + // WORKFLOW: Run main workflow + // + NFCORE_CRISPRSEQ ( + PIPELINE_INITIALISATION.out.samplesheet + ) + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + NFCORE_CRISPRSEQ.out.multiqc_report + ) } /* diff --git a/modules.json b/modules.json index c91b896f..8d813172 100644 --- a/modules.json +++ b/modules.json @@ -5,22 +5,36 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, "fastqc": { "branch": "master", - "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", + "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", "installed_by": ["modules"] } } + }, + "subworkflows": { + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + } + } } } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index ec0e11b2..00000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -process SAMPLESHEET_CHECK { - tag "$samplesheet" - label 'process_single' - - conda "conda-forge::python=3.8.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" - - input: - path samplesheet - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // This script is bundled with the pipeline, in nf-core/crisprseq/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml deleted file mode 100644 index 9b3272bc..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: custom_dumpsoftwareversions -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf deleted file mode 100644 index f2187611..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ /dev/null @@ -1,24 +0,0 @@ -process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_single' - - // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" - - input: - path versions - - output: - path "software_versions.yml" , emit: yml - path "software_versions_mqc.yml", emit: mqc_yml - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - template 'dumpsoftwareversions.py' -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml deleted file mode 100644 index 5f15a5fd..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ /dev/null @@ -1,37 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: custom_dumpsoftwareversions -description: Custom module used to dump software versions within the nf-core pipeline template -keywords: - - custom - - dump - - version -tools: - - custom: - description: Custom module used to dump software versions within the nf-core pipeline template - homepage: https://github.com/nf-core/tools - documentation: https://github.com/nf-core/tools - licence: ["MIT"] -input: - - versions: - type: file - description: YML file containing software versions - pattern: "*.yml" -output: - - yml: - type: file - description: Standard YML file containing software versions - pattern: "software_versions.yml" - - mqc_yml: - type: file - description: MultiQC custom content YML file containing software versions - pattern: "software_versions_mqc.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" -maintainers: - - "@drpatelh" - - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py deleted file mode 100755 index e55b8d43..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ /dev/null @@ -1,102 +0,0 @@ -#!/usr/bin/env python - - -"""Provide functions to merge multiple versions.yml files.""" - - -import platform -from textwrap import dedent - -import yaml - - -def _make_versions_html(versions): - """Generate a tabular HTML output of all versions for MultiQC.""" - html = [ - dedent( - """\\ - -
    Process Name \\", + " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    - - - - - - - - """ - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f"""\\ - - - - - - """ - ) - ) - html.append("") - html.append("
    Process Name Software Version
    {process if (i == 0) else ''}{tool}{version}
    ") - return "\\n".join(html) - - -def main(): - """Load all version files and generate merged output.""" - versions_this_module = {} - versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, - } - - with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - - # aggregate versions by the module name (derived from fully-qualified process name) - versions_by_module = {} - for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - - versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", - } - - versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), - } - - with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) - with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - - with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) - - -if __name__ == "__main__": - main() diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test deleted file mode 100644 index b1e1630b..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ /dev/null @@ -1,43 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" - script "../main.nf" - process "CUSTOM_DUMPSOFTWAREVERSIONS" - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "dumpsoftwareversions" - tag "custom/dumpsoftwareversions" - - test("Should run without failures") { - when { - process { - """ - def tool1_version = ''' - TOOL1: - tool1: 0.11.9 - '''.stripIndent() - - def tool2_version = ''' - TOOL2: - tool2: 1.9 - '''.stripIndent() - - input[0] = Channel.of(tool1_version, tool2_version).collectFile() - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.versions, - file(process.out.mqc_yml[0]).readLines()[0..10], - file(process.out.yml[0]).readLines()[0..7] - ).match() - } - ) - } - } -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap deleted file mode 100644 index 5f59a936..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ /dev/null @@ -1,33 +0,0 @@ -{ - "Should run without failures": { - "content": [ - [ - "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" - ], - [ - "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", - " \\n\\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n \\n \\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n\\n\\n \\n\\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\" - ], - [ - "CUSTOM_DUMPSOFTWAREVERSIONS:", - " python: 3.11.7", - " yaml: 5.4.1", - "TOOL1:", - " tool1: 0.11.9", - "TOOL2:", - " tool2: '1.9'", - "Workflow:" - ] - ], - "timestamp": "2024-01-09T23:01:18.710682" - } -} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml deleted file mode 100644 index 405aa24a..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/dumpsoftwareversions: - - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 1f21c664..70edae4d 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -33,7 +33,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_single") } ) } } @@ -63,7 +63,7 @@ nextflow_process { { assert path(process.out.html[0][1][0]).text.contains("") }, { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } ) } } @@ -89,7 +89,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } ) } } @@ -115,7 +115,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } ) } } @@ -153,7 +153,7 @@ nextflow_process { { assert path(process.out.html[0][1][2]).text.contains("") }, { assert path(process.out.html[0][1][3]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } ) } } @@ -179,7 +179,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } ) } } @@ -204,7 +204,7 @@ nextflow_process { { assert process.success }, { assert snapshot(process.out.html.collect { file(it[1]).getName() } + process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match() } + process.out.versions ).match("fastqc_stub") } ) } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 5d624bb8..86f7c311 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,5 +1,17 @@ { - "sarscov2 single-end [fastq] - stub": { + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { "content": [ [ "test.html", @@ -7,14 +19,70 @@ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:40:57.254299" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" }, - "versions": { + "fastqc_versions_custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:36:50.033627" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 7625b752..2212096a 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.19 + - bioconda::multiqc=1.20 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1b9f7c43..354f4430 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : + 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index d0438eda..f1c4242e 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -3,6 +3,7 @@ nextflow_process { name "Test Process MULTIQC" script "../main.nf" process "MULTIQC" + tag "modules" tag "modules_nfcore" tag "multiqc" @@ -12,7 +13,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -25,7 +26,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_single") } ) } @@ -36,7 +37,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] @@ -49,7 +50,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_config") } ) } } @@ -61,7 +62,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -75,7 +76,7 @@ nextflow_process { { assert snapshot(process.out.report.collect { file(it).getName() } + process.out.data.collect { file(it).getName() } + process.out.plots.collect { file(it).getName() } + - process.out.versions ).match() } + process.out.versions ).match("multiqc_stub") } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index d37e7304..c204b488 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -1,21 +1,41 @@ { - "versions": { + "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:02:49.911994" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:28:51.744211298" }, - "sarscov2 single-end [fastqc] - stub": { + "multiqc_stub": { "content": [ [ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:03:14.524346" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:28.847433492" + }, + "multiqc_versions_config": { + "content": [ + [ + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:13.223621555" } } \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index d9fae6c8..817d2a71 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,9 +16,7 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - - - // MultiQC options + fasta = null// MultiQC options multiqc_config = null multiqc_title = null multiqc_logo = null @@ -43,7 +41,6 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - // Max resource options // Defaults only, expecting to be overwritten diff --git a/nextflow_schema.json b/nextflow_schema.json index 1c1a6e97..bfea1e1d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -16,6 +16,7 @@ "type": "string", "format": "file-path", "exists": true, + "schema": "assets/schema_input.json", "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", "description": "Path to comma-separated file containing information about the samples in the experiment.", diff --git a/pyproject.toml b/pyproject.toml index 7d08e1c8..56110621 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -3,11 +3,13 @@ [tool.ruff] line-length = 120 target-version = "py38" -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] cache-dir = "~/.cache/ruff" -[tool.ruff.isort] +[tool.ruff.lint] +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] + +[tool.ruff.lint.isort] known-first-party = ["nf_core"] -[tool.ruff.per-file-ignores] +[tool.ruff.lint.per-file-ignores] "__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf deleted file mode 100644 index 0aecf87f..00000000 --- a/subworkflows/local/input_check.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// Check input samplesheet and get read channels -// - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta -} diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf new file mode 100644 index 00000000..dc90ad90 --- /dev/null +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -0,0 +1,247 @@ +// +// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { fromSamplesheet } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir // string: The output directory where the results will be saved + input // string: Path to input samplesheet + + main: + + ch_versions = Channel.empty() + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = nfCoreLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + // + // Custom validation for pipeline parameters + // + validateInputParameters() + + // + // Create channel from input file provided through params.input + // + Channel + .fromSamplesheet("input") + .map { + meta, fastq_1, fastq_2 -> + if (!fastq_2) { + return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] + } else { + return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] + } + } + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .map { + meta, fastqs -> + return [ meta, fastqs.flatten() ] + } + .set { ch_samplesheet } + + emit: + samplesheet = ch_samplesheet + versions = ch_versions +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ +// +// Check and validate pipeline parameters +// +def validateInputParameters() { + genomeExistsError() +}// +// Validate channels from input samplesheet +// +def validateInputSamplesheet(input) { + def (metas, fastqs) = input[1..2] + + // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + if (!endedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") + } + + return [ metas[0], fastqs ] +} +// +// Get attribute from genome config file e.g. fasta +// +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} + +// +// Exit pipeline if incorrect --genome key provided +// +def genomeExistsError() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } +}// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + + + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 00000000..ac31f28f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 00000000..e5c3a0a8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..8ed4310c --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..db2030f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,12 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "timestamp": "2024-01-19T11:32:36.031083" + }, + "Test Function checkCondaChannels": { + "content": null, + "timestamp": "2024-01-19T11:32:50.456" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..f7c54bc6 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,123 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = params.outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = null + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 00000000..53574ffe --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 00000000..f8476112 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 00000000..a8b55d6f --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,440 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " ${workflow.manifest.doi}\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 00000000..d08d2434 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..1dc317f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..10f948e6 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,138 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "timestamp": "2024-02-09T15:43:55.145717" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "timestamp": "2024-01-19T11:34:13.548431224" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "timestamp": "2024-01-19T11:34:38.840454873" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "timestamp": "2024-01-19T11:34:22.24352016" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "timestamp": "2024-01-19T11:35:04.418416984" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "timestamp": "2024-01-19T11:34:55.420000755" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "timestamp": "2024-01-19T11:35:13.436366565" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..8940d32d --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 00000000..d07ce54c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,15 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "timestamp": "2024-01-19T11:35:22.538940073" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 00000000..d0a926bf --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 00000000..ac8523c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 00000000..2585b65d --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 00000000..3d4a6b04 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 00000000..517ee54e --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 00000000..7626c1c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 00000000..60b1cfff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/workflows/crisprseq.nf b/workflows/crisprseq.nf index d49e6e2b..467da216 100644 --- a/workflows/crisprseq.nf +++ b/workflows/crisprseq.nf @@ -1,54 +1,15 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - PRINT PARAMS SUMMARY + IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -WorkflowCrisprseq.initialise(params, log) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT LOCAL MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT NF-CORE MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// MODULE: Installed directly from nf-core/modules -// -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_crisprseq_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -56,50 +17,45 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// Info required for completion email and summary -def multiqc_report = [] - workflow CRISPRSEQ { - ch_versions = Channel.empty() + take: + ch_samplesheet // channel: samplesheet read in from --input - // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files - // - INPUT_CHECK ( - file(params.input) - ) - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema + main: + + ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() // // MODULE: Run FastQC // FASTQC ( - INPUT_CHECK.out.reads + ch_samplesheet ) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') - ) + // + // Collate and save software versions + // + softwareVersionsToYAML(ch_versions) + .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) + .set { ch_collated_versions } // // MODULE: MultiQC // - workflow_summary = WorkflowCrisprseq.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowCrisprseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) MULTIQC ( ch_multiqc_files.collect(), @@ -107,31 +63,10 @@ workflow CRISPRSEQ { ch_multiqc_custom_config.toList(), ch_multiqc_logo.toList() ) - multiqc_report = MULTIQC.out.report.toList() -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.dump_parameters(workflow, params) - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) - } -} -workflow.onError { - if (workflow.errorReport.contains("Process requirement exceeds available memory")) { - println("🛑 Default resources exceed availability 🛑 ") - println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") - } + emit: + multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [ path(versions.yml) ] } /* From 23f261c7e16c571cfc3111f9d2c5f4d93a042d4d Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Wed, 21 Feb 2024 11:21:10 +0100 Subject: [PATCH 059/226] Add bowtie --- .../nf-core/mageck/count/mageck-count.diff | 18 +++---- modules/nf-core/mageck/count/main.nf | 2 +- workflows/crisprseq_screening.nf | 54 ++++++++++++------- 3 files changed, 42 insertions(+), 32 deletions(-) diff --git a/modules/nf-core/mageck/count/mageck-count.diff b/modules/nf-core/mageck/count/mageck-count.diff index 9becf832..70e991e0 100644 --- a/modules/nf-core/mageck/count/mageck-count.diff +++ b/modules/nf-core/mageck/count/mageck-count.diff @@ -9,49 +9,43 @@ Changes in module 'nf-core/mageck/count' conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - @@ -8,13 +8,16 @@ 'biocontainers/mageck:0.5.9--py37h6bb024c_0' }" input: - tuple val(meta), path(inputfile) + tuple val(meta), path(fastq1), path(fastq2) - path(library) output: - tuple val(meta), path("*count*.txt"), emit: count -+ tuple val(meta), path("*count.txt"), emit: count ++ tuple val(meta), path("*count.txt") , emit: count tuple val(meta), path("*.count_normalized.txt"), emit: norm - path "versions.yml" , emit: versions -+ tuple val(meta), path("*.countsummary.txt"), emit: summary ++ tuple val(meta), path("*.countsummary.txt") , emit: summary + tuple val(meta), path("*.count_normalized.txt"), emit: normalized -+ tuple val(meta), path("*.log"), emit: logs -+ path "versions.yml", emit: versions ++ tuple val(meta), path("*.log") , emit: logs ++ path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when -@@ -22,9 +25,15 @@ - +@@ -22,8 +25,14 @@ script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def input_file = ("$inputfile".endsWith(".fastq.gz")) ? "--fastq ${inputfile}" : "-k ${inputfile}" - def sample_label = ("$inputfile".endsWith(".fastq.gz") || "$inputfile".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' -- + // def input_file = ("$inputfile".endsWith(".fastq.gz") || "$inputfile".endsWith(".fq.gz")) ? "--fastq ${inputfile}" : "-k ${inputfile}" + def sample_label = ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' + -+ if (meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz"))) { ++ if (meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz") || "$fastq1".endsWith(".bam"))) { + input = "--fastq $fastq1" - + } else { + input = "--fastq $fastq1 --fastq-2 $fastq2" + } """ mageck \\ - count \\ @@ -32,7 +41,7 @@ -l $library \\ -n $prefix \\ diff --git a/modules/nf-core/mageck/count/main.nf b/modules/nf-core/mageck/count/main.nf index d48d9a30..7adf037e 100644 --- a/modules/nf-core/mageck/count/main.nf +++ b/modules/nf-core/mageck/count/main.nf @@ -28,7 +28,7 @@ process MAGECK_COUNT { // def input_file = ("$inputfile".endsWith(".fastq.gz") || "$inputfile".endsWith(".fq.gz")) ? "--fastq ${inputfile}" : "-k ${inputfile}" def sample_label = ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' - if (meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz"))) { + if (meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz") || "$fastq1".endsWith(".bam"))) { input = "--fastq $fastq1" } else { input = "--fastq $fastq1 --fastq-2 $fastq2" diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 0c56af2d..22082195 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -131,34 +131,52 @@ workflow CRISPRSEQ_SCREENING { } .set { ch_input } + // + // MODULE: Run FastQC + // + FASTQC ( + ch_input + ) + ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + //set adapter seq to null to make it compatible with crispr targeted ch_cutadapt = ch_input.combine(Channel.value([[]])) - if(params.three_prime_adapter) { - CUTADAPT_THREE_PRIME( - ch_input_cutadapt - ) - ch_cutadapt = CUTADAPT_THREE_PRIME.out.reads - ch_versions = ch_versions.mix(CUTADAPT_THREE_PRIME.out.versions) - } if(params.five_prime_adapter) { CUTADAPT_FIVE_PRIME( ch_cutadapt ) - ch_cutadapt = CUTADAPT_FIVE_PRIME.out.reads - ch_versions = ch_versions.mix(CUTADAPT.out.versions) + CUTADAPT_FIVE_PRIME.out.reads.combine(Channel.value([[]])).set { ch_cutadapt } + ch_cutadapt.map{ meta, fastq, proto -> + meta.id = "${meta.id}_trim" + [meta, [fastq], proto] + }.set { ch_cutadapt } + + ch_versions = ch_versions.mix(CUTADAPT_FIVE_PRIME.out.versions) } + if(params.three_prime_adapter) { + CUTADAPT_THREE_PRIME( + ch_cutadapt + ) + ch_cutadapt = CUTADAPT_THREE_PRIME.out.reads.combine(Channel.value([[]])) + ch_versions = ch_versions.mix(CUTADAPT_THREE_PRIME.out.versions) + } + + if(params.five_prime_adapter || params.three_prime_adapter) { ch_cutadapt - .map{ meta, fastq -> + .map{ meta, fastq, empty -> [meta, [fastq]] } .set { ch_input } } if(params.fasta){ - ch_fasta = Channel.fromPath(params.fasta) + Channel.of("fasta") + .combine(Channel.fromPath(params.fasta)) + .set{ ch_fasta } + BOWTIE2_BUILD(ch_fasta) ch_versions = ch_versions.mix(BOWTIE2_BUILD.out.versions) @@ -168,15 +186,11 @@ workflow CRISPRSEQ_SCREENING { false, false ) - } - // - // MODULE: Run FastQC - // - FASTQC ( - ch_input - ) - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + BOWTIE2_ALIGN.out.aligned.map{ meta, bam -> + [meta, [bam]] + }.set{ch_input} + } // this is to concatenate everything for mageck count ch_input @@ -201,6 +215,8 @@ workflow CRISPRSEQ_SCREENING { .last() .set { joined } + joined.dump(tag: "DUMP") + // // MODULE: Run mageck count // From 61a05dcf96a22fc6da7eceae982f484056070e24 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Wed, 21 Feb 2024 14:21:42 +0100 Subject: [PATCH 060/226] Add docs --- docs/output/screening.md | 20 +++++++++++++++++--- docs/usage/screening.md | 7 ++++++- 2 files changed, 23 insertions(+), 4 deletions(-) diff --git a/docs/output/screening.md b/docs/output/screening.md index aa163333..746e276a 100644 --- a/docs/output/screening.md +++ b/docs/output/screening.md @@ -17,14 +17,16 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Preprocessing](#preprocessing) - [FastQC](#fastqc) - Read Quality Control - [cutadapt](#cutadapt) - Trimming reads from fastq files +- [Mapping](#mapping) - bowtie2 aligned reads - [Counting](#counting) - - [MAGeCK count](#mageck-count) - Mapping reads to reference + - [MAGeCK count](#mageck-count) - Mapping reads to reference library - [CNV correction](#cnv-correction)) - [CRISPRcleanR](#crisprcleanr-normalization) - Copy Number Variation correction and read normalization in case of knock-out screens. - [Gene essentiality](#gene-essentiality-computation) - [MAGeCK rra](#mageck-rra) - modified robust ranking aggregation (RRA) algorithm - [MAGeCK mle](#mageck-mle) - maximum-likelihood estimation (MLE) for robust identification of CRISPR-screen hits - [BAGEL2](#BAGEL2) - Bayes Factor to identify essential genes + - [MAGeCKFlute](#flutemle) - graphics to visualise MAGECK MLE output - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution @@ -57,6 +59,18 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d [cutadapt](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/). Cutadapt finds and removes adapter sequences, primers, poly-A tails and other types of unwanted sequence from your high-throughput sequencing reads. MAGeCK count normally automatically detects adapter sequences and trims, however if trimming lengths are different, cutadapt can be used, as mentioned [here](https://sourceforge.net/p/mageck/wiki/advanced_tutorial/). For further reading and documentation see the [cutadapt helper page](https://cutadapt.readthedocs.io/en/stable/guide.html). +## Alignment + +
    +Output files + +- `bowtie2/` + - `*.log`: log file of the command ran and the output + - `*.bam`: bam file + - `*.bowtie2`: index from bowtie2 from the provided fasta file + +
    + ## Counting ### MAGeCK count @@ -108,8 +122,8 @@ For further reading and documentation see the [cutadapt helper page](https://cut - `*_count_sgrna_summary.txt`: sgRNA ranking results, tab separated file containing means, p-values - `*.report.Rmd`: markdown report recapping essential genes - `*_count_table.log`: log of the run - - `*_scatterview.png`: scatter view of the targeted genes and their logFC - - `*_rank.png`: rank view of the targeted genes + - `*_scatterview.png`: scatter view of the targeted genes in the library and their logFC + - `*_rank.png`: rank view of the targeted genes in the library diff --git a/docs/usage/screening.md b/docs/usage/screening.md index ba7fcc47..3ab85d80 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -44,7 +44,12 @@ An [example samplesheet](https://github.com/nf-core/test-datasets/blob/crisprseq ### cutadapt -MAGeCK count which is the main alignment software used is normally able to automatically determine the trimming length and sgRNA length, in most cases. Therefore, you don't need to go to this step unless MAGeCK fails to do so by itself. If the nucleotide length in front of sgRNA varies between different reads, you can use cutadapt to remove the adaptor sequences by using the flag `--cutadapt ADAPTER`. +MAGeCK count which is the main alignment software used is normally able to automatically determine the trimming length and sgRNA length, in most cases. Therefore, you don't need to go to this step unless MAGeCK fails to do so by itself. If the nucleotide length in front of sgRNA varies between different reads, you can use cutadapt to remove the adaptor sequences by using the flag `--five_prime_adapter` or `--three_prime_adapter` . + + +### bowtie2 + +The MAGeCK count module supports bam files, which allows you to align with bowtie2 first. If you wish to do so (for instance to allow library with mismatches or to set the aligner with specific flags) you can provide a fasta file with `--fasta`. Currently, you also still need to provide the library file. ### library From 4a57dbd6bd661ac461fe81598963293e01eec3b2 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 21 Feb 2024 13:49:43 +0000 Subject: [PATCH 061/226] [automated] Fix linting with Prettier --- docs/usage/screening.md | 1 - 1 file changed, 1 deletion(-) diff --git a/docs/usage/screening.md b/docs/usage/screening.md index 3ab85d80..68bc145d 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -46,7 +46,6 @@ An [example samplesheet](https://github.com/nf-core/test-datasets/blob/crisprseq MAGeCK count which is the main alignment software used is normally able to automatically determine the trimming length and sgRNA length, in most cases. Therefore, you don't need to go to this step unless MAGeCK fails to do so by itself. If the nucleotide length in front of sgRNA varies between different reads, you can use cutadapt to remove the adaptor sequences by using the flag `--five_prime_adapter` or `--three_prime_adapter` . - ### bowtie2 The MAGeCK count module supports bam files, which allows you to align with bowtie2 first. If you wish to do so (for instance to allow library with mismatches or to set the aligner with specific flags) you can provide a fasta file with `--fasta`. Currently, you also still need to provide the library file. From 0e9d858dae476609e051102fe09652c0ab6d99f0 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Wed, 21 Feb 2024 14:51:04 +0100 Subject: [PATCH 062/226] Ran pre commit --- docs/usage/screening.md | 1 - workflows/crisprseq_screening.nf | 22 +++++++++++----------- 2 files changed, 11 insertions(+), 12 deletions(-) diff --git a/docs/usage/screening.md b/docs/usage/screening.md index 3ab85d80..68bc145d 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -46,7 +46,6 @@ An [example samplesheet](https://github.com/nf-core/test-datasets/blob/crisprseq MAGeCK count which is the main alignment software used is normally able to automatically determine the trimming length and sgRNA length, in most cases. Therefore, you don't need to go to this step unless MAGeCK fails to do so by itself. If the nucleotide length in front of sgRNA varies between different reads, you can use cutadapt to remove the adaptor sequences by using the flag `--five_prime_adapter` or `--three_prime_adapter` . - ### bowtie2 The MAGeCK count module supports bam files, which allows you to align with bowtie2 first. If you wish to do so (for instance to allow library with mismatches or to set the aligner with specific flags) you can provide a fasta file with `--fasta`. Currently, you also still need to provide the library file. diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 22082195..4dd75ac9 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -340,9 +340,9 @@ workflow CRISPRSEQ_SCREENING { } } - //CUSTOM_DUMPSOFTWAREVERSIONS ( - // ch_versions.unique{ it.text }.collectFile(name: 'collated_versions.yml') - //) + CUSTOM_DUMPSOFTWAREVERSIONS ( + ch_versions.unique{ it.text }.collectFile(name: 'collated_versions.yml') + ) // // MODULE: MultiQC @@ -363,14 +363,14 @@ workflow CRISPRSEQ_SCREENING { ch_multiqc_files = channel.empty() } - //MULTIQC ( - // ch_multiqc_files.collect(), - // ch_multiqc_config.collect().ifEmpty([]), - //ch_multiqc_custom_config.collect().ifEmpty([]), - //ch_multiqc_logo.collect().ifEmpty([]) - //) - //multiqc_report = MULTIQC.out.report.toList() - //h_versions = ch_versions.mix(MULTIQC.out.versions) + MULTIQC ( + ch_multiqc_files.collect(), + ch_multiqc_config.collect().ifEmpty([]), + ch_multiqc_custom_config.collect().ifEmpty([]), + ch_multiqc_logo.collect().ifEmpty([]) + ) + multiqc_report = MULTIQC.out.report.toList() + ch_versions = ch_versions.mix(MULTIQC.out.versions) } /* From 246a04045ccb00cc6ff19a524b9bc0dc94fe33cf Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Thu, 22 Feb 2024 14:30:44 +0100 Subject: [PATCH 063/226] Update docs/output/screening.md MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Júlia Mir Pedrol --- docs/output/screening.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/output/screening.md b/docs/output/screening.md index 746e276a..27bbf16f 100644 --- a/docs/output/screening.md +++ b/docs/output/screening.md @@ -17,7 +17,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Preprocessing](#preprocessing) - [FastQC](#fastqc) - Read Quality Control - [cutadapt](#cutadapt) - Trimming reads from fastq files -- [Mapping](#mapping) - bowtie2 aligned reads +- [Mapping](#alignment) - bowtie2 aligned reads - [Counting](#counting) - [MAGeCK count](#mageck-count) - Mapping reads to reference library - [CNV correction](#cnv-correction)) From 7edd959414d83e91e7932e265a82ca394bb4e361 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Thu, 22 Feb 2024 14:32:00 +0100 Subject: [PATCH 064/226] Update workflows/crisprseq_screening.nf --- workflows/crisprseq_screening.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 4dd75ac9..0ee48b2c 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -215,7 +215,7 @@ workflow CRISPRSEQ_SCREENING { .last() .set { joined } - joined.dump(tag: "DUMP") + // // MODULE: Run mageck count From 408375d9d479ab57b4b81d89adfebca7f955582c Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Fri, 23 Feb 2024 10:59:43 +0100 Subject: [PATCH 065/226] Update workflows/crisprseq_screening.nf MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Júlia Mir Pedrol --- workflows/crisprseq_screening.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 0ee48b2c..03b7fc23 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -356,7 +356,7 @@ workflow CRISPRSEQ_SCREENING { ch_multiqc_files = Channel.empty() ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - //ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) + ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) if(!params.count_table) { ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) } else { From f6b3687259089b8c167c7a2ca9ca5fcc05f34f8a Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Fri, 23 Feb 2024 11:03:13 +0100 Subject: [PATCH 066/226] Change suggested --- CHANGELOG.md | 1 + nextflow_schema.json | 12 +++++++----- workflows/crisprseq_screening.nf | 5 ++++- 3 files changed, 12 insertions(+), 6 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 4785e9fa..10d60e89 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,6 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Template update to 2.11.1 ([#105](https://github.com/nf-core/crisprseq/pull/105)) - Added a csv input option for crisprcleanr ([#105](https://github.com/nf-core/crisprseq/pull/105)) - Added a contrasts parameter so the pipeline automatically creates design matrices and MAGeCK MLE ([#109](https://github.com/nf-core/crisprseq/pull/109)) +- Added bowtie2 and three prime and five prime adapter trimming ([#103](https://github.com/nf-core/crisprseq/pull/103)) ## [v2.1.1 - Jamon Salas - patch](https://github.com/nf-core/crisprseq/releases/tag/2.1.1) - [14.12.2023] diff --git a/nextflow_schema.json b/nextflow_schema.json index 06dada55..09140482 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -160,14 +160,16 @@ "description": "sgRNA and targetting genes, tab separated" }, "five_prime_adapter": { - "type": "string" + "type": "string", + "description": "Sequencing adapter sequence to use for trimming on the 5' end" }, "three_prime_adapter": { "type": "string", - "description": "Sequencing adapter sequence to use for trimming." + "description": "Sequencing adapter sequence to use for trimming on the 3' end" }, "fasta": { - "type": "string" + "type": "string", + "description": "Fasta file in case you want to map with bowtie2 and then MAGeCK count" }, "mle_design_matrix": { "type": "string", @@ -200,12 +202,12 @@ "min_reads": { "type": "number", "description": "a filter threshold value for sgRNAs, based on their average counts in the control sample", - "default": 30.0 + "default": 30 }, "min_targeted_genes": { "type": "number", "description": "Minimal number of different genes targeted by sgRNAs in a biased segment in order for the corresponding counts to be corrected for CRISPRcleanR", - "default": 3.0 + "default": 3 }, "bagel_reference_essentials": { "type": "string", diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 03b7fc23..8560079a 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -187,6 +187,9 @@ workflow CRISPRSEQ_SCREENING { false ) + ch_versions = ch_versions.mix(BOWTIE2_ALIGN.out.versions) + + BOWTIE2_ALIGN.out.aligned.map{ meta, bam -> [meta, [bam]] }.set{ch_input} @@ -215,7 +218,7 @@ workflow CRISPRSEQ_SCREENING { .last() .set { joined } - + // // MODULE: Run mageck count From f73a24314340fe2d5d8351e8a575e0410de12961 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 29 Feb 2024 16:10:12 +0000 Subject: [PATCH 067/226] Template update for nf-core/tools version 2.13.1 --- .devcontainer/devcontainer.json | 10 +---- .github/CONTRIBUTING.md | 14 ++++--- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/ci.yml | 2 +- .github/workflows/download_pipeline.yml | 2 +- .github/workflows/linting.yml | 2 +- .github/workflows/release-announcements.yml | 2 +- .gitpod.yml | 6 +-- README.md | 3 +- modules.json | 8 ++-- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- .../nf-core/multiqc/tests/main.nf.test.snap | 12 +++--- .../utils_nfcore_crisprseq_pipeline/main.nf | 10 +++-- .../tests/main.function.nf.test | 2 +- .../tests/main.function.nf.test.snap | 12 +++++- .../tests/main.workflow.nf.test | 20 ++------- .../tests/nextflow.config | 2 +- .../tests/main.function.nf.test.snap | 42 +++++++++++++++---- .../tests/main.workflow.nf.test.snap | 6 ++- .../tests/main.nf.test | 2 +- 23 files changed, 99 insertions(+), 74 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe3..b290e090 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -10,15 +10,7 @@ "vscode": { // Set *default* container specific settings.json values on container create. "settings": { - "python.defaultInterpreterPath": "/opt/conda/bin/python", - "python.linting.enabled": true, - "python.linting.pylintEnabled": true, - "python.formatting.autopep8Path": "/opt/conda/bin/autopep8", - "python.formatting.yapfPath": "/opt/conda/bin/yapf", - "python.linting.flake8Path": "/opt/conda/bin/flake8", - "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", - "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.defaultInterpreterPath": "/opt/conda/bin/python" }, // Add the IDs of extensions you want installed when the container is created. diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 3de1bef3..600f7055 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,9 +9,8 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -:::info -If you need help using or modifying nf-core/crisprseq then the best place to ask is on the nf-core Slack [#crisprseq](https://nfcore.slack.com/channels/crisprseq) channel ([join our Slack here](https://nf-co.re/join/slack)). -::: +> [!NOTE] +> If you need help using or modifying nf-core/crisprseq then the best place to ask is on the nf-core Slack [#crisprseq](https://nfcore.slack.com/channels/crisprseq) channel ([join our Slack here](https://nf-co.re/join/slack)). ## Contribution workflow @@ -27,8 +26,11 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests -You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to -receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. +You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: + +```bash +nf-test test --profile debug,test,docker --verbose +``` When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -90,7 +92,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. ### Naming schemes diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 1ae44009..c6709972 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/cris - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/crisprseq/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/crisprseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 82f78840..b393817c 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 539cd12f..f46f773a 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index f3fb085a..f7646db6 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -31,7 +31,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 with: version: "${{ matrix.NXF_VER }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index f823210d..08622fd5 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -28,7 +28,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 748b4311..073e1876 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -35,7 +35,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index c3674af2..d468aeaa 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -12,7 +12,7 @@ jobs: - name: get topics and convert to hashtags id: get_topics run: | - curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT - uses: rzr/fediverse-action@master with: diff --git a/.gitpod.yml b/.gitpod.yml index 363d5b1d..105a1821 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -10,13 +10,11 @@ tasks: vscode: extensions: # based on nf-core.nf-core-extensionpack - - codezombiech.gitignore # Language support for .gitignore files - # - cssho.vscode-svgviewer # SVG viewer - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code - - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar - mechatroner.rainbow-csv # Highlight columns in csv files in different colors - # - nextflow.nextflow # Nextflow syntax highlighting + # - nextflow.nextflow # Nextflow syntax highlighting - oderwat.indent-rainbow # Highlight indentation level - streetsidesoftware.code-spell-checker # Spelling checker for source code + - charliermarsh.ruff # Code linter Ruff diff --git a/README.md b/README.md index 9e23efde..f5ad96c3 100644 --- a/README.md +++ b/README.md @@ -7,12 +7,13 @@ [![GitHub Actions CI Status](https://github.com/nf-core/crisprseq/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/crisprseq/actions/workflows/ci.yml) [![GitHub Actions Linting Status](https://github.com/nf-core/crisprseq/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/crisprseq/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/crisprseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/crisprseq) +[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/crisprseq) [![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23crisprseq-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/crisprseq)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) diff --git a/modules.json b/modules.json index 8d813172..609fe705 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", "installed_by": ["modules"] } } @@ -21,17 +21,17 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] } } diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 2212096a..ca39fb67 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.20 + - bioconda::multiqc=1.21 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 354f4430..47ac352f 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : - 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : + 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index c204b488..bfebd802 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:28:51.744211298" + "timestamp": "2024-02-29T08:48:55.657331" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:28.847433492" + "timestamp": "2024-02-29T08:49:49.071937" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:13.223621555" + "timestamp": "2024-02-29T08:49:25.457567" } } \ No newline at end of file diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index dc90ad90..42992053 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -1,5 +1,5 @@ // -// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// Subworkflow with functionality specific to the nf-core/crisprseq pipeline // /* @@ -152,7 +152,9 @@ workflow PIPELINE_COMPLETION { // def validateInputParameters() { genomeExistsError() -}// +} + +// // Validate channels from input samplesheet // def validateInputSamplesheet(input) { @@ -190,7 +192,9 @@ def genomeExistsError() { "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" error(error_string) } -}// +} + +// // Generate methods description for MultiQC // def toolCitationText() { diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test index 8ed4310c..68718e4f 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -51,4 +51,4 @@ nextflow_function { ) } } -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap index db2030f8..e3f0baf4 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -3,10 +3,18 @@ "content": [ "v9.9.9" ], - "timestamp": "2024-01-19T11:32:36.031083" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:05.308243" }, "Test Function checkCondaChannels": { "content": null, - "timestamp": "2024-01-19T11:32:50.456" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:12.425833" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test index f7c54bc6..ca964ce8 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -11,9 +11,6 @@ nextflow_workflow { test("Should run no inputs") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = false @@ -39,9 +36,6 @@ nextflow_workflow { test("Should print version") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = true @@ -68,19 +62,16 @@ nextflow_workflow { test("Should dump params") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = 'results' check_conda_channels = false input[0] = false input[1] = true - input[2] = params.outdir + input[2] = outdir input[3] = false """ } @@ -96,19 +87,16 @@ nextflow_workflow { test("Should not create params JSON if no output directory") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = null check_conda_channels = false input[0] = false input[1] = true - input[2] = null + input[2] = outdir input[3] = false """ } diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index 53574ffe..d0a926bf 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -6,4 +6,4 @@ manifest { nextflowVersion = '!>=23.04.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap index 10f948e6..1037232c 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -1,25 +1,41 @@ { "Test Function checkProfileProvided": { "content": null, - "timestamp": "2024-02-09T15:43:55.145717" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:03.360873" }, "Test Function checkConfigProvided": { "content": [ true ], - "timestamp": "2024-01-19T11:34:13.548431224" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:59.729647" }, "Test Function nfCoreLogo": { "content": [ "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" ], - "timestamp": "2024-01-19T11:34:38.840454873" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:10.562934" }, "Test Function workflowCitation": { "content": [ "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" ], - "timestamp": "2024-01-19T11:34:22.24352016" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:07.019761" }, "Test Function without logColours": { "content": [ @@ -73,13 +89,21 @@ "biwhite": "" } ], - "timestamp": "2024-01-19T11:35:04.418416984" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:17.969323" }, "Test Function dashedLine": { "content": [ "-\u001b[2m----------------------------------------------------\u001b[0m-" ], - "timestamp": "2024-01-19T11:34:55.420000755" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:14.366181" }, "Test Function with logColours": { "content": [ @@ -133,6 +157,10 @@ "biwhite": "\u001b[1;97m" } ], - "timestamp": "2024-01-19T11:35:13.436366565" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:21.714424" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap index d07ce54c..859d1030 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -10,6 +10,10 @@ ] } ], - "timestamp": "2024-01-19T11:35:22.538940073" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:25.726491" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test index 517ee54e..5784a33f 100644 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -197,4 +197,4 @@ nextflow_workflow { ) } } -} \ No newline at end of file +} From 401e90629589d4b3cd11fb858c5937f8d48e1f1a Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Wed, 6 Mar 2024 11:00:10 +0100 Subject: [PATCH 068/226] adapt cutadapt to work with single and paired end again --- .github/workflows/awsfulltest.yml | 2 +- .nf-core.yml | 4 +- conf/modules.config | 28 ++++++++----- ..._full.config => test_targeted_full.config} | 3 ++ modules/nf-core/cutadapt/cutadapt.diff | 39 ++++++------------- modules/nf-core/cutadapt/main.nf | 8 ++-- nextflow.config | 3 +- workflows/crisprseq_targeted.nf | 5 +++ 8 files changed, 50 insertions(+), 42 deletions(-) rename conf/{test_full.config => test_targeted_full.config} (95%) diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 391c769b..ddca75d7 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -27,7 +27,7 @@ jobs: "hook_url": "${{ secrets.MEGATESTS_ALERTS_SLACK_HOOK_URL }}", "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/crisprseq/results-${{ github.sha }}/targeted_test" } - profiles: test_full + profiles: test_targeted_full - uses: actions/upload-artifact@v4 with: diff --git a/.nf-core.yml b/.nf-core.yml index 085dbd0a..2c087061 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,6 +1,8 @@ repository_type: pipeline lint: files_exist: - - conf/test.config # We skip these linting as we have splitted tests between targeted and screening + # We skip the linting of these files as we have splitted tests between targeted and screening + - conf/test.config + - conf/test_full.config files_unchanged: - lib/NfcoreTemplate.groovy # Introduced a change ahead of the nf-core/tools release diff --git a/conf/modules.config b/conf/modules.config index 03c310ae..4abae14e 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -134,15 +134,25 @@ process { } withName: CUTADAPT { - if(params.analysis == 'targeted') { - ext.args = '-g file:overrepresented.fasta -N' - } - if(params.analysis == 'screening' && params.three_prime_adapter) { - ext.args = "-a ${params.three_prime_adapter}" - } - if(params.analysis == 'screening' && params.five_prime_adapter) { - ext.args = "-g ${params.five_prime_adapter}" - } + ext.args = '-g file:overrepresented.fasta -N' + publishDir = [ + path: { "${params.outdir}/preprocessing/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: CUTADAPT_FIVE_PRIME { + ext.args = "-g ${params.five_prime_adapter}" + publishDir = [ + path: { "${params.outdir}/preprocessing/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: CUTADAPT_THREE_PRIME { + ext.args = "-a ${params.three_prime_adapter}" publishDir = [ path: { "${params.outdir}/preprocessing/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, mode: params.publish_dir_mode, diff --git a/conf/test_full.config b/conf/test_targeted_full.config similarity index 95% rename from conf/test_full.config rename to conf/test_targeted_full.config index ba79a208..f3622137 100644 --- a/conf/test_full.config +++ b/conf/test_targeted_full.config @@ -20,4 +20,7 @@ params { // Aligner aligner = 'minimap2' + + // Steps + overrepresented = true } diff --git a/modules/nf-core/cutadapt/cutadapt.diff b/modules/nf-core/cutadapt/cutadapt.diff index a69969df..5ccfbfe2 100644 --- a/modules/nf-core/cutadapt/cutadapt.diff +++ b/modules/nf-core/cutadapt/cutadapt.diff @@ -1,7 +1,7 @@ Changes in module 'nf-core/cutadapt' --- modules/nf-core/cutadapt/main.nf +++ modules/nf-core/cutadapt/main.nf -@@ -8,11 +8,11 @@ +@@ -8,7 +8,7 @@ 'biocontainers/cutadapt:3.4--py39h38f01e4_1' }" input: @@ -9,36 +9,21 @@ Changes in module 'nf-core/cutadapt' + tuple val(meta), path(reads), path(adapter_seq) output: -- tuple val(meta), path('*.trim.fastq.gz'), emit: reads -- tuple val(meta), path('*.log') , emit: log -+ tuple val(meta), path('*.trim.fastq.gz'), optional: true, emit: reads -+ tuple val(meta), path('*.log') , optional: true, emit: log - path "versions.yml" , emit: versions - - when: -@@ -21,14 +21,21 @@ - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" -- def trimmed = meta.single_end ? "-o ${prefix}.trim.fastq.gz" : "-o ${prefix}_1.trim.fastq.gz -p ${prefix}_2.trim.fastq.gz" -+ if (adapter_seq != []) - """ - cutadapt \\ - --cores $task.cpus \\ - $args \\ -- $trimmed \\ -+ -o ${prefix}.trim.fastq.gz \\ + tuple val(meta), path('*.trim.fastq.gz'), emit: reads +@@ -29,12 +29,13 @@ + $trimmed \\ $reads \\ > ${prefix}.cutadapt.log -+ cat <<-END_VERSIONS > versions.yml -+ "${task.process}": -+ cutadapt: \$(cutadapt --version) -+ END_VERSIONS -+ """ -+ else -+ """ ++ cat <<-END_VERSIONS > versions.yml "${task.process}": cutadapt: \$(cutadapt --version) + END_VERSIONS + """ +- ++ + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def trimmed = meta.single_end ? "${prefix}.trim.fastq.gz" : "${prefix}_1.trim.fastq.gz ${prefix}_2.trim.fastq.gz" ************************************************************ diff --git a/modules/nf-core/cutadapt/main.nf b/modules/nf-core/cutadapt/main.nf index 2c6bf763..1a0145e4 100644 --- a/modules/nf-core/cutadapt/main.nf +++ b/modules/nf-core/cutadapt/main.nf @@ -11,8 +11,8 @@ process CUTADAPT { tuple val(meta), path(reads), path(adapter_seq) output: - tuple val(meta), path('*.trim.fastq.gz'), optional: true, emit: reads - tuple val(meta), path('*.log') , optional: true, emit: log + tuple val(meta), path('*.trim.fastq.gz'), emit: reads + tuple val(meta), path('*.log') , emit: log path "versions.yml" , emit: versions when: @@ -21,13 +21,15 @@ process CUTADAPT { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + def trimmed = meta.single_end ? "-o ${prefix}.trim.fastq.gz" : "-o ${prefix}_1.trim.fastq.gz -p ${prefix}_2.trim.fastq.gz" """ cutadapt \\ --cores $task.cpus \\ $args \\ - -o ${prefix}.trim.fastq.gz \\ + $trimmed \\ $reads \\ > ${prefix}.cutadapt.log + cat <<-END_VERSIONS > versions.yml "${task.process}": cutadapt: \$(cutadapt --version) diff --git a/nextflow.config b/nextflow.config index 4ca1a12e..2334082b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -200,7 +200,8 @@ profiles { } test { includeConfig 'conf/test_targeted.config' } test_targeted { includeConfig 'conf/test_targeted.config' } - test_full { includeConfig 'conf/test_full.config' } + test_full { includeConfig 'conf/test_targeted_full.config' } + test_targeted_full { includeConfig 'conf/test_targeted_full.config' } test_umis { includeConfig 'conf/test_umis.config' } test_screening_full { includeConfig 'conf/test_screening_full.config' } test_screening { includeConfig 'conf/test_screening.config' } diff --git a/workflows/crisprseq_targeted.nf b/workflows/crisprseq_targeted.nf index 4138470f..c3048aa7 100644 --- a/workflows/crisprseq_targeted.nf +++ b/workflows/crisprseq_targeted.nf @@ -278,6 +278,11 @@ workflow CRISPRSEQ_TARGETED { ch_cat_fastq.paired ) .assembled + .map { + // Set single_end to true for the assembled reads + meta, assembled -> + return [ meta - meta.subMap('single_end') + [ single_end:true ], assembled ] + } .mix( ch_cat_fastq.single ) .set { ch_pear_fastq } ch_versions = ch_versions.mix(PEAR.out.versions) From e7f3f24ec880ad2effecaf9629300fcd95e09589 Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Wed, 6 Mar 2024 11:03:53 +0100 Subject: [PATCH 069/226] update changelog --- CHANGELOG.md | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 10d60e89..d3ed94aa 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -12,6 +12,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Added a contrasts parameter so the pipeline automatically creates design matrices and MAGeCK MLE ([#109](https://github.com/nf-core/crisprseq/pull/109)) - Added bowtie2 and three prime and five prime adapter trimming ([#103](https://github.com/nf-core/crisprseq/pull/103)) +### Fixed + +- Adapt cutadapt module to work with single-end and paired-end reads again ([#121](https://github.com/nf-core/crisprseq/pull/121)) + ## [v2.1.1 - Jamon Salas - patch](https://github.com/nf-core/crisprseq/releases/tag/2.1.1) - [14.12.2023] ### Added From 12552d20f2617f5c912df1cfa77b547342a3d18c Mon Sep 17 00:00:00 2001 From: Laurence Kuhlburger Date: Fri, 8 Mar 2024 14:19:19 +0100 Subject: [PATCH 070/226] fix cutadapt --- modules.json | 90 ++++++++++++++----- .../nf-core/mageck/count/mageck-count.diff | 2 +- modules/nf-core/mageck/count/main.nf | 2 +- workflows/crisprseq_screening.nf | 10 +-- 4 files changed, 72 insertions(+), 32 deletions(-) diff --git a/modules.json b/modules.json index b7928c26..44d4fbc9 100644 --- a/modules.json +++ b/modules.json @@ -8,120 +8,164 @@ "bowtie2/align": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bowtie2/build": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/mem": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cat/fastq": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cutadapt": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/cutadapt/cutadapt.diff" }, "fastqc": { "branch": "master", "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "mageck/count": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/count/mageck-count.diff" }, "mageck/mle": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" }, "mageck/test": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/test/mageck-test.diff" }, "medaka": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/medaka/medaka.diff" }, "minimap2/align": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" }, "minimap2/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "multiqc": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "pear": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "racon": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/index": { "branch": "master", "git_sha": "5394565c5fe4c760e5b35977ec7607c62e81d1f8", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "seqtk/seq": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "vsearch/cluster": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" }, "vsearch/sort": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] } } }, @@ -130,4 +174,4 @@ } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/mageck/count/mageck-count.diff b/modules/nf-core/mageck/count/mageck-count.diff index 70e991e0..541f87cc 100644 --- a/modules/nf-core/mageck/count/mageck-count.diff +++ b/modules/nf-core/mageck/count/mageck-count.diff @@ -38,7 +38,7 @@ Changes in module 'nf-core/mageck/count' + // def input_file = ("$inputfile".endsWith(".fastq.gz") || "$inputfile".endsWith(".fq.gz")) ? "--fastq ${inputfile}" : "-k ${inputfile}" + def sample_label = ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' + -+ if (meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz") || "$fastq1".endsWith(".bam"))) { ++ if ((meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) || "$fastq1".endsWith(".bam"))) { + input = "--fastq $fastq1" + } else { + input = "--fastq $fastq1 --fastq-2 $fastq2" diff --git a/modules/nf-core/mageck/count/main.nf b/modules/nf-core/mageck/count/main.nf index 7adf037e..ee98ed29 100644 --- a/modules/nf-core/mageck/count/main.nf +++ b/modules/nf-core/mageck/count/main.nf @@ -28,7 +28,7 @@ process MAGECK_COUNT { // def input_file = ("$inputfile".endsWith(".fastq.gz") || "$inputfile".endsWith(".fq.gz")) ? "--fastq ${inputfile}" : "-k ${inputfile}" def sample_label = ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' - if (meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz") || "$fastq1".endsWith(".bam"))) { + if ((meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) || "$fastq1".endsWith(".bam"))) { input = "--fastq $fastq1" } else { input = "--fastq $fastq1 --fastq-2 $fastq2" diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 8560079a..1c6a94a6 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -141,17 +141,15 @@ workflow CRISPRSEQ_SCREENING { //set adapter seq to null to make it compatible with crispr targeted ch_cutadapt = ch_input.combine(Channel.value([[]])) - if(params.five_prime_adapter) { CUTADAPT_FIVE_PRIME( ch_cutadapt ) CUTADAPT_FIVE_PRIME.out.reads.combine(Channel.value([[]])).set { ch_cutadapt } - ch_cutadapt.map{ meta, fastq, proto -> + ch_cutadapt.map{ meta, fastq, proto -> meta.id = "${meta.id}_trim" - [meta, [fastq], proto] + [meta, fastq, proto] }.set { ch_cutadapt } - ch_versions = ch_versions.mix(CUTADAPT_FIVE_PRIME.out.versions) } @@ -167,7 +165,7 @@ workflow CRISPRSEQ_SCREENING { if(params.five_prime_adapter || params.three_prime_adapter) { ch_cutadapt .map{ meta, fastq, empty -> - [meta, [fastq]] + [meta, fastq] } .set { ch_input } } @@ -218,8 +216,6 @@ workflow CRISPRSEQ_SCREENING { .last() .set { joined } - - // // MODULE: Run mageck count // From 7be435ef908b98046b50fdbbd9d62932864b5374 Mon Sep 17 00:00:00 2001 From: Laurence Kuhlburger Date: Fri, 8 Mar 2024 14:24:43 +0100 Subject: [PATCH 071/226] Add PR in changelog --- CHANGELOG.md | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index d3ed94aa..0479264f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,7 +10,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Template update to 2.11.1 ([#105](https://github.com/nf-core/crisprseq/pull/105)) - Added a csv input option for crisprcleanr ([#105](https://github.com/nf-core/crisprseq/pull/105)) - Added a contrasts parameter so the pipeline automatically creates design matrices and MAGeCK MLE ([#109](https://github.com/nf-core/crisprseq/pull/109)) -- Added bowtie2 and three prime and five prime adapter trimming ([#103](https://github.com/nf-core/crisprseq/pull/103)) +- Added bowtie2 and three prime and five prime adapter trimming ([#103](https://github.com/nf-core/crisprseq/pull/103) and [#123](https://github.com/nf-core/crisprseq/pull/123) +) ### Fixed From bf462dfe612d0cf0718eec2cc40789292d1b948c Mon Sep 17 00:00:00 2001 From: Laurence Kuhlburger Date: Fri, 8 Mar 2024 14:31:06 +0100 Subject: [PATCH 072/226] Ran pre commit --- CHANGELOG.md | 2 +- modules.json | 90 ++++++++------------------------ workflows/crisprseq_screening.nf | 2 +- 3 files changed, 25 insertions(+), 69 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 0479264f..794ea696 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -11,7 +11,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Added a csv input option for crisprcleanr ([#105](https://github.com/nf-core/crisprseq/pull/105)) - Added a contrasts parameter so the pipeline automatically creates design matrices and MAGeCK MLE ([#109](https://github.com/nf-core/crisprseq/pull/109)) - Added bowtie2 and three prime and five prime adapter trimming ([#103](https://github.com/nf-core/crisprseq/pull/103) and [#123](https://github.com/nf-core/crisprseq/pull/123) -) + ) ### Fixed diff --git a/modules.json b/modules.json index 44d4fbc9..b7928c26 100644 --- a/modules.json +++ b/modules.json @@ -8,164 +8,120 @@ "bowtie2/align": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bowtie2/build": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cat/fastq": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cutadapt": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/cutadapt/cutadapt.diff" }, "fastqc": { "branch": "master", "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "mageck/count": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/count/mageck-count.diff" }, "mageck/mle": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" }, "mageck/test": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/test/mageck-test.diff" }, "medaka": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/medaka/medaka.diff" }, "minimap2/align": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" }, "minimap2/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "multiqc": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "pear": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "racon": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/index": { "branch": "master", "git_sha": "5394565c5fe4c760e5b35977ec7607c62e81d1f8", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "seqtk/seq": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "vsearch/cluster": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" }, "vsearch/sort": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } }, @@ -174,4 +130,4 @@ } } } -} \ No newline at end of file +} diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 1c6a94a6..a2905b90 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -146,7 +146,7 @@ workflow CRISPRSEQ_SCREENING { ch_cutadapt ) CUTADAPT_FIVE_PRIME.out.reads.combine(Channel.value([[]])).set { ch_cutadapt } - ch_cutadapt.map{ meta, fastq, proto -> + ch_cutadapt.map{ meta, fastq, proto -> meta.id = "${meta.id}_trim" [meta, fastq, proto] }.set { ch_cutadapt } From 1ec01d993940a3df8aa09b49bdf0c7d48bec7daf Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Fri, 8 Mar 2024 16:06:21 +0100 Subject: [PATCH 073/226] update both workflows according to the template update --- conf/base.config | 3 - modules.json | 40 ------ workflows/crisprseq_screening.nf | 211 +++++++++++-------------------- workflows/crisprseq_targeted.nf | 146 ++++++--------------- 4 files changed, 112 insertions(+), 288 deletions(-) diff --git a/conf/base.config b/conf/base.config index a0ac558c..5ff6a2fb 100644 --- a/conf/base.config +++ b/conf/base.config @@ -59,7 +59,4 @@ process { errorStrategy = 'retry' maxRetries = 2 } - withName:CUSTOM_DUMPSOFTWAREVERSIONS { - cache = false - } } diff --git a/modules.json b/modules.json index 59ce0e23..3846e879 100644 --- a/modules.json +++ b/modules.json @@ -36,11 +36,6 @@ "installed_by": ["modules"], "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" }, - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, "cutadapt": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", @@ -87,41 +82,6 @@ "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "mageck/count": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/count/mageck-count.diff" - }, - "mageck/mle": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" - }, - "mageck/test": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/test/mageck-test.diff" - }, - "medaka": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/medaka/medaka.diff" - }, - "minimap2/align": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" - }, - "minimap2/index": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 8560079a..6eb4234e 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -1,93 +1,14 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - PRINT PARAMS SUMMARY + IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap; fromSamplesheet } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -WorkflowCrisprseq.initialise(params, log) - -// Set screening parameters and channels -if (params.library) { ch_library = file(params.library) } -if (params.crisprcleanr) { - if(params.crisprcleanr.endsWith(".csv")) { - ch_crisprcleanr = Channel.fromPath(params.crisprcleanr) - } else { - ch_crisprcleanr = Channel.value(params.crisprcleanr) - } -} - -if(params.mle_design_matrix) { - Channel.fromPath(params.mle_design_matrix) - .set { ch_design } -} - -if(params.rra && params.mle_design_matrix) { - warning "mle_design_matrix will only be used for the MAGeCK MLE computations" - } - -if(params.fasta && params.count_table) { - error "Please provide either a fasta file or a count_table" - } - -if(params.fasta && !params.library) { - error "Please provide a fasta file and the library file" - } - -if(params.rra && params.mle_design_matrix) { - warning "mle_design_matrix will only be used for the MAGeCK MLE computations" - } - -if(params.rra && !params.contrasts) { - error "Please also provide the contrasts table to compare the samples for MAGeCK RRA" - } - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT LOCAL MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - - -*/ - -include { BAGEL2_FC } from '../modules/local/bagel2/fc' -include { BAGEL2_BF } from '../modules/local/bagel2/bf' -include { BAGEL2_PR } from '../modules/local/bagel2/pr' -include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' -include { MATRICESCREATION } from '../modules/local/matricescreation' - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT NF-CORE MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// MODULE: Installed directly from nf-core/modules -// +include { BAGEL2_FC } from '../modules/local/bagel2/fc' +include { BAGEL2_BF } from '../modules/local/bagel2/bf' +include { BAGEL2_PR } from '../modules/local/bagel2/pr' +include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' +include { MATRICESCREATION } from '../modules/local/matricescreation' include { FASTQC } from '../modules/nf-core/fastqc/main' include { CUTADAPT as CUTADAPT_THREE_PRIME } from '../modules/nf-core/cutadapt/main' include { CUTADAPT as CUTADAPT_FIVE_PRIME } from '../modules/nf-core/cutadapt/main' @@ -96,7 +17,6 @@ include { MAGECK_COUNT } from '../modules/nf-core/mageck/co include { MAGECK_MLE } from '../modules/nf-core/mageck/mle/main' include { MAGECK_TEST } from '../modules/nf-core/mageck/test/main' include { MAGECK_GRAPHRRA } from '../modules/local/mageck/graphrra' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' include { CRISPRCLEANR_NORMALIZE } from '../modules/nf-core/crisprcleanr/normalize/main' include { MAGECK_MLE as MAGECK_MLE_MATRIX } from '../modules/nf-core/mageck/mle/main' include { BOWTIE2_BUILD } from '../modules/nf-core/bowtie2/build/main' @@ -108,18 +28,53 @@ include { BOWTIE2_ALIGN } from '../modules/nf-core/bowtie2/a ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// Info required for completion email and summary -def multiqc_report = [] - workflow CRISPRSEQ_SCREENING { + take: + ch_samplesheet // channel: samplesheet read in from --input + + main: + + // Set screening parameters and channels ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() + + if (params.library) { ch_library = file(params.library) } + if (params.crisprcleanr) { + if(params.crisprcleanr.endsWith(".csv")) { + ch_crisprcleanr = Channel.fromPath(params.crisprcleanr) + } else { + ch_crisprcleanr = Channel.value(params.crisprcleanr) + } + } + + if(params.mle_design_matrix) { + Channel.fromPath(params.mle_design_matrix) + .set { ch_design } + } + + if(params.rra && params.mle_design_matrix) { + warning "mle_design_matrix will only be used for the MAGeCK MLE computations" + } + + if(params.fasta && params.count_table) { + error "Please provide either a fasta file or a count_table" + } + + if(params.fasta && !params.library) { + error "Please provide a fasta file and the library file" + } + + if(params.rra && params.mle_design_matrix) { + warning "mle_design_matrix will only be used for the MAGeCK MLE computations" + } + + if(params.rra && !params.contrasts) { + error "Please also provide the contrasts table to compare the samples for MAGeCK RRA" + } if(!params.count_table){ - // - // Create input channel from input file provided through params.input - // - Channel.fromSamplesheet("input") + ch_samplesheet .map{ meta, fastq_1, fastq_2, x, y, z -> // x (reference), y (protospacer), and z (template) are part of the targeted workflows and we don't need them if (fastq_2) { @@ -137,6 +92,7 @@ workflow CRISPRSEQ_SCREENING { FASTQC ( ch_input ) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) //set adapter seq to null to make it compatible with crispr targeted @@ -152,6 +108,7 @@ workflow CRISPRSEQ_SCREENING { [meta, [fastq], proto] }.set { ch_cutadapt } + ch_multiqc_files = ch_multiqc_files.mix(CUTADAPT.out.log.collect{it[1]}) ch_versions = ch_versions.mix(CUTADAPT_FIVE_PRIME.out.versions) } @@ -160,6 +117,7 @@ workflow CRISPRSEQ_SCREENING { ch_cutadapt ) ch_cutadapt = CUTADAPT_THREE_PRIME.out.reads.combine(Channel.value([[]])) + ch_multiqc_files = ch_multiqc_files.mix(CUTADAPT.out.log.collect{it[1]}) ch_versions = ch_versions.mix(CUTADAPT_THREE_PRIME.out.versions) } @@ -343,58 +301,35 @@ workflow CRISPRSEQ_SCREENING { } } - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique{ it.text }.collectFile(name: 'collated_versions.yml') - ) + // + // Collate and save software versions + // + softwareVersionsToYAML(ch_versions) + .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) + .set { ch_collated_versions } // // MODULE: MultiQC // - workflow_summary = WorkflowCrisprseq.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowCrisprseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - if(!params.count_table) { - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - } else { - ch_multiqc_files = channel.empty() - } + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) MULTIQC ( ch_multiqc_files.collect(), - ch_multiqc_config.collect().ifEmpty([]), - ch_multiqc_custom_config.collect().ifEmpty([]), - ch_multiqc_logo.collect().ifEmpty([]) + ch_multiqc_config.toList(), + ch_multiqc_custom_config.toList(), + ch_multiqc_logo.toList() ) - multiqc_report = MULTIQC.out.report.toList() - ch_versions = ch_versions.mix(MULTIQC.out.versions) -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.dump_parameters(workflow, params) - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.adaptivecard(workflow, params, summary_params, projectDir, log) - } + emit: + multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [ path(versions.yml) ] } - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - THE END -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ diff --git a/workflows/crisprseq_targeted.nf b/workflows/crisprseq_targeted.nf index c3048aa7..c7b52d64 100644 --- a/workflows/crisprseq_targeted.nf +++ b/workflows/crisprseq_targeted.nf @@ -1,65 +1,20 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - PRINT PARAMS SUMMARY + IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap; fromSamplesheet } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -WorkflowCrisprseq.initialise(params, log) -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT LOCAL MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// - -// -// MODULE -// -include { FIND_ADAPTERS } from '../modules/local/find_adapters' -include { EXTRACT_UMIS } from '../modules/local/extract_umis' -include { ORIENT_REFERENCE } from '../modules/local/orient_reference' -include { CIGAR_PARSER } from '../modules/local/cigar_parser' -include { PREPROCESSING_SUMMARY } from '../modules/local/preprocessing_summary' -include { CLUSTERING_SUMMARY } from '../modules/local/clustering_summary' -include { ALIGNMENT_SUMMARY } from '../modules/local/alignment_summary' -include { TEMPLATE_REFERENCE } from '../modules/local/template_reference' -include { CRISPRSEQ_PLOTTER } from '../modules/local/crisprseq_plotter' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT NF-CORE MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// MODULE: Installed directly from nf-core/modules -// +include { FIND_ADAPTERS } from '../modules/local/find_adapters' +include { EXTRACT_UMIS } from '../modules/local/extract_umis' +include { ORIENT_REFERENCE } from '../modules/local/orient_reference' +include { CIGAR_PARSER } from '../modules/local/cigar_parser' +include { PREPROCESSING_SUMMARY } from '../modules/local/preprocessing_summary' +include { CLUSTERING_SUMMARY } from '../modules/local/clustering_summary' +include { ALIGNMENT_SUMMARY } from '../modules/local/alignment_summary' +include { TEMPLATE_REFERENCE } from '../modules/local/template_reference' +include { CRISPRSEQ_PLOTTER } from '../modules/local/crisprseq_plotter' include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' include { PEAR } from '../modules/nf-core/pear/main' include { CAT_FASTQ } from '../modules/nf-core/cat/fastq/main' include { SEQTK_SEQ as SEQTK_SEQ_MASK } from '../modules/nf-core/seqtk/seq/main' @@ -81,7 +36,6 @@ include { MEDAKA } from '../modules/nf-core/m include { CUTADAPT } from '../modules/nf-core/cutadapt/main' include { SAMTOOLS_INDEX } from '../modules/nf-core/samtools/index/main' - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ DEFINE GROOVY FUNCTIONS @@ -127,17 +81,17 @@ def umi_to_sequence_centroid(cluster) { ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// Info required for completion email and summary -def multiqc_report = [] - workflow CRISPRSEQ_TARGETED { + take: + ch_samplesheet // channel: samplesheet read in from --input + + main: + ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() - // - // Create input channel from input file provided through params.input - // - Channel.fromSamplesheet("input") + ch_samplesheet .multiMap { meta, fastq_1, fastq_2, reference, protospacer, template -> // meta.condition is part of the screening workflow and we need to remove it if (fastq_2) { @@ -293,6 +247,7 @@ workflow CRISPRSEQ_TARGETED { FASTQC ( ch_pear_fastq ) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) ch_trimmed = Channel.empty() @@ -324,6 +279,7 @@ workflow CRISPRSEQ_TARGETED { CUTADAPT ( ch_adapter_seqs.adapters ) + ch_multiqc_files = ch_multiqc_files.mix(CUTADAPT.out.log.collect{it[1]}) ch_versions = ch_versions.mix(CUTADAPT.out.versions) ch_adapter_seqs.no_adapters @@ -748,6 +704,9 @@ workflow CRISPRSEQ_TARGETED { CIGAR_PARSER ( ch_to_parse_cigar ) + ch_multiqc_files = ch_multiqc_files.mix(CIGAR_PARSER.out.processing.collect{it[1]}) + ch_multiqc_files = ch_multiqc_files.mix(CIGAR_PARSER.out.edition.collect{it[2]}) + ch_multiqc_files = ch_multiqc_files.mix(CIGAR_PARSER.out.qcindels.collect{it[1]}) ch_versions = ch_versions.mix(CIGAR_PARSER.out.versions.first()) @@ -763,32 +722,25 @@ workflow CRISPRSEQ_TARGETED { // - // MODULE: Dump software versions + // Collate and save software versions // - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique{ it.text }.collectFile(name: 'collated_versions.yml') - ) + softwareVersionsToYAML(ch_versions) + .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) + .set { ch_collated_versions } // // MODULE: MultiQC // - workflow_summary = WorkflowCrisprseq.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowCrisprseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CIGAR_PARSER.out.processing.collect{it[1]}.ifEmpty([])) - ch_multiqc_files = ch_multiqc_files.mix(CIGAR_PARSER.out.edition.collect{it[2]}.ifEmpty([])) - ch_multiqc_files = ch_multiqc_files.mix(CIGAR_PARSER.out.qcindels.collect{it[1]}.ifEmpty([])) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - if (params.overrepresented) { - ch_multiqc_files = ch_multiqc_files.mix(CUTADAPT.out.log.collect{it[1]}.ifEmpty([])) - } + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) MULTIQC ( ch_multiqc_files.collect(), @@ -796,28 +748,8 @@ workflow CRISPRSEQ_TARGETED { ch_multiqc_custom_config.toList(), ch_multiqc_logo.toList() ) - multiqc_report = MULTIQC.out.report.toList() -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.dump_parameters(workflow, params) - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) - } + emit: + multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [ path(versions.yml) ] } - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - THE END -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ From d7a93386f3f545386d3204a0198823692276697b Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Mon, 11 Mar 2024 11:54:35 +0100 Subject: [PATCH 074/226] use utils_nfcore_criprseq_pipeline to format input channel --- conf/modules.config | 8 --- main.nf | 25 +++++++-- .../utils_nfcore_crisprseq_pipeline/main.nf | 53 +++++++++++++------ workflows/crisprseq_screening.nf | 16 +++--- workflows/crisprseq_targeted.nf | 52 ++++++++---------- 5 files changed, 87 insertions(+), 67 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 4abae14e..bb43c626 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -316,14 +316,6 @@ process { ] } - withName: CUSTOM_DUMPSOFTWAREVERSIONS { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - pattern: '*_versions.yml' - ] - } - withName: 'MULTIQC' { ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ diff --git a/main.nf b/main.nf index 56a67b3c..f1a43efe 100644 --- a/main.nf +++ b/main.nf @@ -44,20 +44,31 @@ include { CRISPRSEQ_SCREENING } from './workflows/crisprseq_screening' workflow NFCORE_CRISPRSEQ { take: - samplesheet // channel: samplesheet read in from --input + reads_targeted // channel: fastqc files read in from --input + reads_screening // channel: fastqc files read in from --input + reference // channel: reference sequence read from --input + protospacer // channel: protospacer sequence read from --input + template // channel: template sequence read from --input main: // // WORKFLOW: Run pipeline // if ( params.analysis == "targeted" ) { - CRISPRSEQ_TARGETED (samplesheet) + CRISPRSEQ_TARGETED ( + reads_targeted, + reference, + template, + protospacer + ) + multiqc_report_ch = CRISPRSEQ_TARGETED.out.multiqc_report } else if ( params.analysis == "screening" ) { - CRISPRSEQ_SCREENING (samplesheet) + CRISPRSEQ_SCREENING (reads_screening) + multiqc_report_ch = CRISPRSEQ_SCREENING.out.multiqc_report } emit: - multiqc_report = CRISPRSEQ.out.multiqc_report // channel: /path/to/multiqc_report.html + multiqc_report = multiqc_report_ch // channel: /path/to/multiqc_report.html } /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -86,7 +97,11 @@ workflow { // WORKFLOW: Run main workflow // NFCORE_CRISPRSEQ ( - PIPELINE_INITIALISATION.out.samplesheet + PIPELINE_INITIALISATION.out.reads_targeted, + PIPELINE_INITIALISATION.out.fastqc_screening, + PIPELINE_INITIALISATION.out.reference, + PIPELINE_INITIALISATION.out.protospacer, + PIPELINE_INITIALISATION.out.template ) // diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index 42992053..4fb60d5f 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -82,26 +82,37 @@ workflow PIPELINE_INITIALISATION { // Channel .fromSamplesheet("input") - .map { - meta, fastq_1, fastq_2 -> - if (!fastq_2) { - return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] + .multiMap { + meta, fastq_1, fastq_2, reference, protospacer, template -> + if (fastq_2) { + files = [ fastq_1, fastq_2 ] } else { - return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] + files = [ fastq_1 ] } + reads_targeted: [ meta.id, meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], files ] + reads_screening:[ meta + [ single_end:fastq_2?false:true ], files ] + reference: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], reference] + protospacer: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], protospacer] + template: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], template] } - .groupTuple() - .map { - validateInputSamplesheet(it) - } - .map { - meta, fastqs -> - return [ meta, fastqs.flatten() ] - } - .set { ch_samplesheet } + .set { ch_input } + + // + // Validate input samplesheet + // + ch_input.reads_targeted + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .set { reads_targeted } emit: - samplesheet = ch_samplesheet + reads_targeted = reads_targeted + fastqc_screening = ch_input.reads_screening + reference = ch_input.reference + protospacer = ch_input.protospacer + template = ch_input.template versions = ch_versions } @@ -166,6 +177,18 @@ def validateInputSamplesheet(input) { error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") } + // Check that multiple runs of the same sample contain a reference or not + def reference_ok = metas.collect{ it.self_reference }.unique().size == 1 + if (!reference_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must all contain a reference or not: ${metas[0].id}") + } + + // Check that multiple runs of the same sample contain a template or not + def template_ok = metas.collect{ it.template }.unique().size == 1 + if (!template_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must all contain a template or not: ${metas[0].id}") + } + return [ metas[0], fastqs ] } // diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 6eb4234e..8130d861 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -4,11 +4,13 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ +// Local modules include { BAGEL2_FC } from '../modules/local/bagel2/fc' include { BAGEL2_BF } from '../modules/local/bagel2/bf' include { BAGEL2_PR } from '../modules/local/bagel2/pr' include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' include { MATRICESCREATION } from '../modules/local/matricescreation' +// nf-core modules include { FASTQC } from '../modules/nf-core/fastqc/main' include { CUTADAPT as CUTADAPT_THREE_PRIME } from '../modules/nf-core/cutadapt/main' include { CUTADAPT as CUTADAPT_FIVE_PRIME } from '../modules/nf-core/cutadapt/main' @@ -21,6 +23,11 @@ include { CRISPRCLEANR_NORMALIZE } from '../modules/nf-core/crisprcle include { MAGECK_MLE as MAGECK_MLE_MATRIX } from '../modules/nf-core/mageck/mle/main' include { BOWTIE2_BUILD } from '../modules/nf-core/bowtie2/build/main' include { BOWTIE2_ALIGN } from '../modules/nf-core/bowtie2/align/main' +// Functions +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_crisprseq_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -75,15 +82,6 @@ workflow CRISPRSEQ_SCREENING { if(!params.count_table){ ch_samplesheet - .map{ meta, fastq_1, fastq_2, x, y, z -> - // x (reference), y (protospacer), and z (template) are part of the targeted workflows and we don't need them - if (fastq_2) { - files = [ fastq_1, fastq_2 ] - } else { - files = [ fastq_1 ] - } - return [ meta + [ single_end:fastq_2?false:true ], files ] - } .set { ch_input } // diff --git a/workflows/crisprseq_targeted.nf b/workflows/crisprseq_targeted.nf index c7b52d64..5cbf3ecb 100644 --- a/workflows/crisprseq_targeted.nf +++ b/workflows/crisprseq_targeted.nf @@ -4,6 +4,7 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ +// Local modules include { FIND_ADAPTERS } from '../modules/local/find_adapters' include { EXTRACT_UMIS } from '../modules/local/extract_umis' include { ORIENT_REFERENCE } from '../modules/local/orient_reference' @@ -13,6 +14,7 @@ include { CLUSTERING_SUMMARY } from '../modules/local/clu include { ALIGNMENT_SUMMARY } from '../modules/local/alignment_summary' include { TEMPLATE_REFERENCE } from '../modules/local/template_reference' include { CRISPRSEQ_PLOTTER } from '../modules/local/crisprseq_plotter' +// nf-core modules include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' include { PEAR } from '../modules/nf-core/pear/main' @@ -35,6 +37,11 @@ include { MINIMAP2_INDEX } from '../modules/nf-core/m include { MEDAKA } from '../modules/nf-core/medaka/main' include { CUTADAPT } from '../modules/nf-core/cutadapt/main' include { SAMTOOLS_INDEX } from '../modules/nf-core/samtools/index/main' +// Functions +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_crisprseq_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -84,35 +91,21 @@ def umi_to_sequence_centroid(cluster) { workflow CRISPRSEQ_TARGETED { take: - ch_samplesheet // channel: samplesheet read in from --input + ch_input_reads // channel: input reads read in from --input + ch_input_reference // channel: reference sequence read in from --input + ch_input_template // channel: template sequence read in from --input + ch_input_protospacer // channel: protospacer sequence read in from --input main: ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() - ch_samplesheet - .multiMap { meta, fastq_1, fastq_2, reference, protospacer, template -> - // meta.condition is part of the screening workflow and we need to remove it - if (fastq_2) { - files = [ fastq_1, fastq_2 ] - } else { - files = [ fastq_1 ] - } - reads: [ meta.id, meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], files ] - reference: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], reference] - protospacer: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], protospacer] - template: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], template] - } - .set { ch_input } - - ch_input - .reads - .groupTuple() - .map { - WorkflowCrisprseq.validateInput(it) - } + // // Separate samples by the ones containing all reads in one file or the ones with many files to be concatenated + // + ch_input_reads + .groupTuple() .branch { meta, fastqs -> single : fastqs.size() == 1 @@ -122,11 +115,10 @@ workflow CRISPRSEQ_TARGETED { } .set { ch_fastq } - // // Add reference sequences to file // - ch_input.reference + ch_input_reference .tap{ meta_reference } .filter{ meta, sequence -> sequence instanceof String } .collectFile() { meta, reference -> @@ -149,7 +141,7 @@ workflow CRISPRSEQ_TARGETED { // // Add template sequences to file // - ch_input.template + ch_input_template .tap{ meta_template } .filter{ meta, sequence -> sequence instanceof String } .collectFile() { meta, template -> @@ -173,7 +165,7 @@ workflow CRISPRSEQ_TARGETED { // to channel: [ meta, reference, protospacer] if (!params.reference_fasta && !params.protospacer) { ch_seq_reference - .join(ch_input.protospacer) + .join(ch_input_protospacer) .set{ reference_protospacer } } else if (!params.reference_fasta) { // If a protospacer was provided through the --protospacer param instead of the samplesheet @@ -184,13 +176,13 @@ workflow CRISPRSEQ_TARGETED { } else if (!params.protospacer) { // If a reference was provided through a fasta file or igenomes instead of the samplesheet ch_reference = Channel.fromPath(params.reference_fasta) - ch_input.protospacer + ch_input_protospacer .combine(ch_reference) .set{ reference_protospacer } } else { ch_reference = Channel.fromPath(params.reference_fasta) ch_protospacer = Channel.of(params.protospacer) - ch_input.reads + ch_input_reads .combine(ch_reference) .combine(ch_protospacer) .set{ reference_protospacer } @@ -678,7 +670,7 @@ workflow CRISPRSEQ_TARGETED { ch_mapped_bam .join(SAMTOOLS_INDEX.out.bai) .join(ORIENT_REFERENCE.out.reference) - .join(ch_input.protospacer) + .join(ch_input_protospacer) .join(ch_seq_template, remainder: true) .join(ch_template_bam, remainder: true) .join(TEMPLATE_REFERENCE.out.fasta, remainder: true) @@ -716,7 +708,7 @@ workflow CRISPRSEQ_TARGETED { CRISPRSEQ_PLOTTER ( CIGAR_PARSER.out.indels .join(ORIENT_REFERENCE.out.reference) - .join(ch_input.protospacer) + .join(ch_input_protospacer) ) ch_versions = ch_versions.mix(CRISPRSEQ_PLOTTER.out.versions.first()) From e10878f04ff22a2a0f43ec38bedc50fe8c59b31f Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Mon, 11 Mar 2024 14:17:51 +0100 Subject: [PATCH 075/226] create channel creation subworkflows in utils_nf-core_Crisprseq_pipeline --- .../utils_nfcore_crisprseq_pipeline/main.nf | 169 ++++++++++++++++++ workflows/crisprseq_screening.nf | 94 ++++------ workflows/crisprseq_targeted.nf | 101 ++--------- 3 files changed, 216 insertions(+), 148 deletions(-) diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index 4fb60d5f..4b3c7b89 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -116,6 +116,153 @@ workflow PIPELINE_INITIALISATION { versions = ch_versions } +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE CHANNELS - SCREENING +======================================================================================== +*/ + +workflow INITIALISATION_CHANNEL_CREATION_SCREENING { + + take: + + main: + + // Library + if (params.library) { + ch_library = file(params.library) + } + + // Crisprcleanr + if (params.crisprcleanr) { + if(params.crisprcleanr.endsWith(".csv")) { + ch_crisprcleanr = Channel.fromPath(params.crisprcleanr) + } else { + ch_crisprcleanr = Channel.value(params.crisprcleanr) + } + } + + // MLE design matrix + if(params.mle_design_matrix) { + ch_design = Channel.fromPath(params.mle_design_matrix) + } + + emit: + library = ch_library // channel: library file + crisprcleanr = ch_crisprcleanr // channel: crisprcleanr file or value + design = ch_design // channel: design matrix file +} + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE CHANNELS - TARGETED +======================================================================================== +*/ + +workflow INITIALISATION_CHANNEL_CREATION_TARGETED { + + take: + input_reads + input_reference + input_template + + main: + + // + // Separate samples by the ones containing all reads in one file or the ones with many files to be concatenated + // + input_reads + .groupTuple() + .branch { + meta, fastqs -> + single : fastqs.size() == 1 + return [ meta, fastqs.flatten() ] + multiple: fastqs.size() > 1 + return [ meta, fastqs.flatten() ] + } + .set { ch_fastq } + + // + // Add reference sequences to file + // + input_reference + .tap{ meta_reference } + .filter{ meta, sequence -> sequence instanceof String } + .collectFile() { meta, reference -> + [ "${meta.id}_reference.fasta", ">${meta.id}\n${reference}\n" ] // Write each reference sequence to a file + } + .map{ new_file -> + [new_file.baseName.split("_reference")[0], new_file] // create a channel with the meta.id and the new file + } + .join(meta_reference + .map{ meta, reference -> + [meta.id, meta] // Join the channel by meta.id with the meta map + } + ) + .map{ metaid, new_file, meta -> + [meta, new_file] // Obtain the final channel with meta map and the new file + } + .set{ ch_seq_reference } + + + // + // Add template sequences to file + // + input_template + .tap{ meta_template } + .filter{ meta, sequence -> sequence instanceof String } + .collectFile() { meta, template -> + [ "${meta.id}_template.fasta", ">${meta.id}\n${template}\n" ] // Write each template sequence to a file + } + .map{ new_file -> + [new_file.baseName.split("_template")[0], new_file] // create a channel with the meta.id and the new file + } + .join(meta_template + .map{ meta, template -> + [meta.id, meta] // Join the channel by meta.id with the meta map + } + ) + .map{ metaid, new_file, meta -> + [meta, new_file] // Obtain the final channel with meta map and the new file + } + .set{ ch_seq_template } + + + // Join channels with reference and protospacer + // to channel: [ meta, reference, protospacer] + if (!params.reference_fasta && !params.protospacer) { + ch_seq_reference + .join(ch_input_protospacer) + .set{ reference_protospacer } + } else if (!params.reference_fasta) { + // If a protospacer was provided through the --protospacer param instead of the samplesheet + ch_protospacer = Channel.of(params.protospacer) + ch_seq_reference + .combine(ch_protospacer) + .set{ reference_protospacer } + } else if (!params.protospacer) { + // If a reference was provided through a fasta file or igenomes instead of the samplesheet + ch_reference = Channel.fromPath(params.reference_fasta) + ch_input_protospacer + .combine(ch_reference) + .set{ reference_protospacer } + } else { + ch_reference = Channel.fromPath(params.reference_fasta) + ch_protospacer = Channel.of(params.protospacer) + ch_input_reads + .combine(ch_reference) + .combine(ch_protospacer) + .set{ reference_protospacer } + } + + emit: + fastq_multiple = ch_fastq.multiple // [ meta, fastqs ] // Channel with the samples with multiple files + fastq_single = ch_fastq.single // [ meta, fastqs ] // Channel with the samples with only one file + template = ch_seq_template // [ meta, template ] // Channel with the template sequences + reference_protospacer = reference_protospacer // [ meta, reference, protospacer] // Channel with the reference and protospacer sequences + +} + /* ======================================================================================== SUBWORKFLOW FOR PIPELINE COMPLETION @@ -272,3 +419,25 @@ def methodsDescriptionText(mqc_methods_yaml) { return description_html.toString() } + +def validateParametersScreening() { + if(params.rra && params.mle_design_matrix) { + warning "mle_design_matrix will only be used for the MAGeCK MLE computations" + } + + if(params.fasta && params.count_table) { + error "Please provide either a fasta file or a count_table" + } + + if(params.fasta && !params.library) { + error "Please provide a fasta file and the library file" + } + + if(params.rra && params.mle_design_matrix) { + warning "mle_design_matrix will only be used for the MAGeCK MLE computations" + } + + if(params.rra && !params.contrasts) { + error "Please also provide the contrasts table to compare the samples for MAGeCK RRA" + } +} diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 8130d861..be6c662e 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -5,29 +5,32 @@ */ // Local modules -include { BAGEL2_FC } from '../modules/local/bagel2/fc' -include { BAGEL2_BF } from '../modules/local/bagel2/bf' -include { BAGEL2_PR } from '../modules/local/bagel2/pr' -include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' -include { MATRICESCREATION } from '../modules/local/matricescreation' +include { BAGEL2_FC } from '../modules/local/bagel2/fc' +include { BAGEL2_BF } from '../modules/local/bagel2/bf' +include { BAGEL2_PR } from '../modules/local/bagel2/pr' +include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' +include { MATRICESCREATION } from '../modules/local/matricescreation' // nf-core modules -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { CUTADAPT as CUTADAPT_THREE_PRIME } from '../modules/nf-core/cutadapt/main' -include { CUTADAPT as CUTADAPT_FIVE_PRIME } from '../modules/nf-core/cutadapt/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { MAGECK_COUNT } from '../modules/nf-core/mageck/count/main' -include { MAGECK_MLE } from '../modules/nf-core/mageck/mle/main' -include { MAGECK_TEST } from '../modules/nf-core/mageck/test/main' -include { MAGECK_GRAPHRRA } from '../modules/local/mageck/graphrra' -include { CRISPRCLEANR_NORMALIZE } from '../modules/nf-core/crisprcleanr/normalize/main' -include { MAGECK_MLE as MAGECK_MLE_MATRIX } from '../modules/nf-core/mageck/mle/main' -include { BOWTIE2_BUILD } from '../modules/nf-core/bowtie2/build/main' -include { BOWTIE2_ALIGN } from '../modules/nf-core/bowtie2/align/main' +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { CUTADAPT as CUTADAPT_THREE_PRIME } from '../modules/nf-core/cutadapt/main' +include { CUTADAPT as CUTADAPT_FIVE_PRIME } from '../modules/nf-core/cutadapt/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { MAGECK_COUNT } from '../modules/nf-core/mageck/count/main' +include { MAGECK_MLE } from '../modules/nf-core/mageck/mle/main' +include { MAGECK_TEST } from '../modules/nf-core/mageck/test/main' +include { MAGECK_GRAPHRRA } from '../modules/local/mageck/graphrra' +include { CRISPRCLEANR_NORMALIZE } from '../modules/nf-core/crisprcleanr/normalize/main' +include { MAGECK_MLE as MAGECK_MLE_MATRIX } from '../modules/nf-core/mageck/mle/main' +include { BOWTIE2_BUILD } from '../modules/nf-core/bowtie2/build/main' +include { BOWTIE2_ALIGN } from '../modules/nf-core/bowtie2/align/main' +// Local subworkflows +include { INITIALISATION_CHANNEL_CREATION_SCREENING } from '../subworkflows/local/utils_nfcore_crisprseq_pipeline' // Functions -include { paramsSummaryMap } from 'plugin/nf-validation' -include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' -include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' -include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_crisprseq_pipeline' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_crisprseq_pipeline' +include { validateParametersScreening } from '../subworkflows/local/utils_nfcore_crisprseq_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -46,43 +49,16 @@ workflow CRISPRSEQ_SCREENING { ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() - if (params.library) { ch_library = file(params.library) } - if (params.crisprcleanr) { - if(params.crisprcleanr.endsWith(".csv")) { - ch_crisprcleanr = Channel.fromPath(params.crisprcleanr) - } else { - ch_crisprcleanr = Channel.value(params.crisprcleanr) - } - } - - if(params.mle_design_matrix) { - Channel.fromPath(params.mle_design_matrix) - .set { ch_design } - } - - if(params.rra && params.mle_design_matrix) { - warning "mle_design_matrix will only be used for the MAGeCK MLE computations" - } - - if(params.fasta && params.count_table) { - error "Please provide either a fasta file or a count_table" - } - - if(params.fasta && !params.library) { - error "Please provide a fasta file and the library file" - } - - if(params.rra && params.mle_design_matrix) { - warning "mle_design_matrix will only be used for the MAGeCK MLE computations" - } + // Validate parameters specific to the screening subworkflow + validateParametersScreening() - if(params.rra && !params.contrasts) { - error "Please also provide the contrasts table to compare the samples for MAGeCK RRA" - } + // + // Initialise channels + // + INITIALISATION_CHANNEL_CREATION_SCREENING() if(!params.count_table){ - ch_samplesheet - .set { ch_input } + ch_input = ch_samplesheet // // MODULE: Run FastQC @@ -181,7 +157,7 @@ workflow CRISPRSEQ_SCREENING { // MAGECK_COUNT ( joined, - ch_library + INITIALISATION_CHANNEL_CREATION_SCREENING.out.library ) ch_versions = ch_versions.mix(MAGECK_COUNT.out.versions.first()) @@ -202,7 +178,7 @@ workflow CRISPRSEQ_SCREENING { CRISPRCLEANR_NORMALIZE( ch_crispr_normalize.collect(), '', - ch_crisprcleanr, + INITIALISATION_CHANNEL_CREATION_SCREENING.out.crisprcleanr, params.min_reads, params.min_targeted_genes ) } else @@ -210,7 +186,7 @@ workflow CRISPRSEQ_SCREENING { ch_crispr_normalize = Channel.of([id: "count_table_normalize"]).concat(ch_counts) CRISPRCLEANR_NORMALIZE( ch_crispr_normalize.collect(), - ch_crisprcleanr, + INITIALISATION_CHANNEL_CREATION_SCREENING.out.crisprcleanr, [], params.min_reads, params.min_targeted_genes) @@ -285,7 +261,7 @@ workflow CRISPRSEQ_SCREENING { if((params.mle_design_matrix) || (params.contrasts && !params.rra)) { if(params.mle_design_matrix) { - ch_design.map { + INITIALISATION_CHANNEL_CREATION_SCREENING.out.design.map { it -> [[id: it.getBaseName()], it] }.set { ch_designed_mle } ch_mle = ch_designed_mle.combine(ch_counts) diff --git a/workflows/crisprseq_targeted.nf b/workflows/crisprseq_targeted.nf index 5cbf3ecb..f9be3c9e 100644 --- a/workflows/crisprseq_targeted.nf +++ b/workflows/crisprseq_targeted.nf @@ -37,6 +37,8 @@ include { MINIMAP2_INDEX } from '../modules/nf-core/m include { MEDAKA } from '../modules/nf-core/medaka/main' include { CUTADAPT } from '../modules/nf-core/cutadapt/main' include { SAMTOOLS_INDEX } from '../modules/nf-core/samtools/index/main' +// Local subworkflows +include { INITIALISATION_CHANNEL_CREATION_TARGETED } from '../subworkflows/local/utils_nfcore_crisprseq_pipeline' // Functions include { paramsSummaryMap } from 'plugin/nf-validation' include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' @@ -102,98 +104,19 @@ workflow CRISPRSEQ_TARGETED { ch_multiqc_files = Channel.empty() // - // Separate samples by the ones containing all reads in one file or the ones with many files to be concatenated + // Initialise channels // - ch_input_reads - .groupTuple() - .branch { - meta, fastqs -> - single : fastqs.size() == 1 - return [ meta, fastqs.flatten() ] - multiple: fastqs.size() > 1 - return [ meta, fastqs.flatten() ] - } - .set { ch_fastq } - - // - // Add reference sequences to file - // - ch_input_reference - .tap{ meta_reference } - .filter{ meta, sequence -> sequence instanceof String } - .collectFile() { meta, reference -> - [ "${meta.id}_reference.fasta", ">${meta.id}\n${reference}\n" ] // Write each reference sequence to a file - } - .map{ new_file -> - [new_file.baseName.split("_reference")[0], new_file] // create a channel with the meta.id and the new file - } - .join(meta_reference - .map{ meta, reference -> - [meta.id, meta] // Join the channel by meta.id with the meta map - } + INITIALISATION_CHANNEL_CREATION_TARGETED( + ch_input_reads, + ch_input_reference, + ch_input_template ) - .map{ metaid, new_file, meta -> - [meta, new_file] // Obtain the final channel with meta map and the new file - } - .set{ ch_seq_reference } - - - // - // Add template sequences to file - // - ch_input_template - .tap{ meta_template } - .filter{ meta, sequence -> sequence instanceof String } - .collectFile() { meta, template -> - [ "${meta.id}_template.fasta", ">${meta.id}\n${template}\n" ] // Write each template sequence to a file - } - .map{ new_file -> - [new_file.baseName.split("_template")[0], new_file] // create a channel with the meta.id and the new file - } - .join(meta_template - .map{ meta, template -> - [meta.id, meta] // Join the channel by meta.id with the meta map - } - ) - .map{ metaid, new_file, meta -> - [meta, new_file] // Obtain the final channel with meta map and the new file - } - .set{ ch_seq_template } - - - // Join channels with reference and protospacer - // to channel: [ meta, reference, protospacer] - if (!params.reference_fasta && !params.protospacer) { - ch_seq_reference - .join(ch_input_protospacer) - .set{ reference_protospacer } - } else if (!params.reference_fasta) { - // If a protospacer was provided through the --protospacer param instead of the samplesheet - ch_protospacer = Channel.of(params.protospacer) - ch_seq_reference - .combine(ch_protospacer) - .set{ reference_protospacer } - } else if (!params.protospacer) { - // If a reference was provided through a fasta file or igenomes instead of the samplesheet - ch_reference = Channel.fromPath(params.reference_fasta) - ch_input_protospacer - .combine(ch_reference) - .set{ reference_protospacer } - } else { - ch_reference = Channel.fromPath(params.reference_fasta) - ch_protospacer = Channel.of(params.protospacer) - ch_input_reads - .combine(ch_reference) - .combine(ch_protospacer) - .set{ reference_protospacer } - } - // // MODULE: Prepare reference sequence // ORIENT_REFERENCE ( - reference_protospacer + INITIALISATION_CHANNEL_CREATION_TARGETED.out.reference_protospacer ) ch_versions = ch_versions.mix(ORIENT_REFERENCE.out.versions) @@ -201,11 +124,11 @@ workflow CRISPRSEQ_TARGETED { // MODULE: Concatenate FastQ files from same sample if required // CAT_FASTQ ( - ch_fastq.multiple + INITIALISATION_CHANNEL_CREATION_TARGETED.out.fastq_multiple ) .reads .groupTuple(by: [0]) - .mix(ch_fastq.single) + .mix(INITIALISATION_CHANNEL_CREATION_TARGETED.out.fastq_single) // Separate samples by paired-end or single-end .branch { meta, fastq -> @@ -638,7 +561,7 @@ workflow CRISPRSEQ_TARGETED { // TEMPLATE_REFERENCE ( ORIENT_REFERENCE.out.reference - .join(ch_seq_template) + .join(INITIALISATION_CHANNEL_CREATION_TARGETED.out.template) ) ch_versions = ch_versions.mix(TEMPLATE_REFERENCE.out.versions.first()) @@ -671,7 +594,7 @@ workflow CRISPRSEQ_TARGETED { .join(SAMTOOLS_INDEX.out.bai) .join(ORIENT_REFERENCE.out.reference) .join(ch_input_protospacer) - .join(ch_seq_template, remainder: true) + .join(INITIALISATION_CHANNEL_CREATION_TARGETED.out.template, remainder: true) .join(ch_template_bam, remainder: true) .join(TEMPLATE_REFERENCE.out.fasta, remainder: true) .join(ALIGNMENT_SUMMARY.out.summary) From f7453de2b76af854b47615c59259c925700a5776 Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Mon, 11 Mar 2024 14:40:16 +0100 Subject: [PATCH 076/226] declare empty channels --- .../local/utils_nfcore_crisprseq_pipeline/main.nf | 11 ++++++++--- workflows/crisprseq_targeted.nf | 3 ++- 2 files changed, 10 insertions(+), 4 deletions(-) diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index 4b3c7b89..c7e437fc 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -128,9 +128,13 @@ workflow INITIALISATION_CHANNEL_CREATION_SCREENING { main: + ch_library = Channel.empty() + ch_crisprcleanr = Channel.empty() + ch_design = Channel.empty() + // Library if (params.library) { - ch_library = file(params.library) + ch_library = Channel.fromPath(params.library) } // Crisprcleanr @@ -165,6 +169,7 @@ workflow INITIALISATION_CHANNEL_CREATION_TARGETED { input_reads input_reference input_template + input_protospacer main: @@ -232,7 +237,7 @@ workflow INITIALISATION_CHANNEL_CREATION_TARGETED { // to channel: [ meta, reference, protospacer] if (!params.reference_fasta && !params.protospacer) { ch_seq_reference - .join(ch_input_protospacer) + .join(input_protospacer) .set{ reference_protospacer } } else if (!params.reference_fasta) { // If a protospacer was provided through the --protospacer param instead of the samplesheet @@ -243,7 +248,7 @@ workflow INITIALISATION_CHANNEL_CREATION_TARGETED { } else if (!params.protospacer) { // If a reference was provided through a fasta file or igenomes instead of the samplesheet ch_reference = Channel.fromPath(params.reference_fasta) - ch_input_protospacer + input_protospacer .combine(ch_reference) .set{ reference_protospacer } } else { diff --git a/workflows/crisprseq_targeted.nf b/workflows/crisprseq_targeted.nf index f9be3c9e..add1f805 100644 --- a/workflows/crisprseq_targeted.nf +++ b/workflows/crisprseq_targeted.nf @@ -109,7 +109,8 @@ workflow CRISPRSEQ_TARGETED { INITIALISATION_CHANNEL_CREATION_TARGETED( ch_input_reads, ch_input_reference, - ch_input_template + ch_input_template, + ch_input_protospacer ) // From 11e4985d4be29b899ef474373936c4385a2daa6a Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Mon, 11 Mar 2024 14:50:39 +0100 Subject: [PATCH 077/226] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index d3ed94aa..babd4ae2 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -11,6 +11,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Added a csv input option for crisprcleanr ([#105](https://github.com/nf-core/crisprseq/pull/105)) - Added a contrasts parameter so the pipeline automatically creates design matrices and MAGeCK MLE ([#109](https://github.com/nf-core/crisprseq/pull/109)) - Added bowtie2 and three prime and five prime adapter trimming ([#103](https://github.com/nf-core/crisprseq/pull/103)) +- Template update to 2.13.1 ([#124](https://github.com/nf-core/crisprseq/pull/124)) ### Fixed From 78e5a5e234d7fab98532a9f63035ac780f822bbe Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Tue, 12 Mar 2024 16:54:05 +0100 Subject: [PATCH 078/226] Update modules/nf-core/mageck/count/main.nf MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Júlia Mir Pedrol --- modules/nf-core/mageck/count/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/nf-core/mageck/count/main.nf b/modules/nf-core/mageck/count/main.nf index ee98ed29..119ea961 100644 --- a/modules/nf-core/mageck/count/main.nf +++ b/modules/nf-core/mageck/count/main.nf @@ -28,7 +28,7 @@ process MAGECK_COUNT { // def input_file = ("$inputfile".endsWith(".fastq.gz") || "$inputfile".endsWith(".fq.gz")) ? "--fastq ${inputfile}" : "-k ${inputfile}" def sample_label = ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' - if ((meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) || "$fastq1".endsWith(".bam"))) { + if (meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) || "$fastq1".endsWith(".bam")) { input = "--fastq $fastq1" } else { input = "--fastq $fastq1 --fastq-2 $fastq2" From df0281649076ef702882e2d904b554f6555eb5c1 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Thu, 14 Mar 2024 12:02:32 +0100 Subject: [PATCH 079/226] Adding day0 as an option for the user --- conf/modules.config | 9 +++++++++ modules/local/mageck/flutemle.nf | 1 - modules/nf-core/mageck/mle/main.nf | 6 ++++-- nextflow.config | 1 + nextflow_schema.json | 4 ++++ workflows/crisprseq_screening.nf | 14 ++++++++++++-- 6 files changed, 30 insertions(+), 5 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 6e9eda03..46e51e67 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -130,6 +130,15 @@ process { containerOptions = '' } + withName: MAGECK_MLE_DAY0 { + ext.args = "--day0-label '${params.day0_label}'" + publishDir = [ + path: { "${params.outdir}/mageck/mle/day0/" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + withName: CRISPRCLEANR_NORMALIZE { publishDir = [ path: { "${params.outdir}/crisprcleanr/normalization/" }, diff --git a/modules/local/mageck/flutemle.nf b/modules/local/mageck/flutemle.nf index efe8ac9e..06416fca 100644 --- a/modules/local/mageck/flutemle.nf +++ b/modules/local/mageck/flutemle.nf @@ -39,7 +39,6 @@ process MAGECK_FLUTEMLE { options(ggrepel.max.overlaps = Inf) mle <- read.table("${gene_summary}", header = TRUE, sep = "\t", stringsAsFactors = FALSE) print(head(mle)) - colnames(mle) #stop(colnames(mle)) FluteMLE(mle, treatname= "${prefix}", proj="${prefix}", pathview.top=0, $args) diff --git a/modules/nf-core/mageck/mle/main.nf b/modules/nf-core/mageck/mle/main.nf index a08a0431..5f5bfdad 100644 --- a/modules/nf-core/mageck/mle/main.nf +++ b/modules/nf-core/mageck/mle/main.nf @@ -21,6 +21,7 @@ process MAGECK_MLE { script: def args = task.ext.args ?: '' prefix = meta.id ?: "${meta.treatment}_vs_${meta.reference}" + def design_command = design_matrix ? "-d $design_matrix" : '' """ @@ -29,8 +30,9 @@ process MAGECK_MLE { $args \\ --threads $task.cpus \\ -k $count_table \\ - -d $design_matrix \\ - -n $prefix + -n $prefix \\ + $design_command + cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/nextflow.config b/nextflow.config index 2334082b..5dc59e1a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -21,6 +21,7 @@ params { count_table = null fasta = null five_prime_adapter = null + day0_label = null three_prime_adapter = null min_reads = 30 min_targeted_genes = 3 diff --git a/nextflow_schema.json b/nextflow_schema.json index 09140482..f2034403 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -171,6 +171,10 @@ "type": "string", "description": "Fasta file in case you want to map with bowtie2 and then MAGeCK count" }, + "day0_label": { + "type": "string", + "description": "Specify the label for control sample (usually day 0 or plasmid). For every other sample label, the module will treat it as a treatment condition and compare with control sample for MAGeCK MLE" + }, "mle_design_matrix": { "type": "string", "format": "file-path", diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index e71637b3..1e4d1dd6 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -42,6 +42,10 @@ if(params.fasta && !params.library) { error "Please provide a fasta file and the library file" } +if(params.day0_label && params.mle_design_matrix) { + warning "MAGeCK MLE module will be ran twice, once with the design matrices and once with day0-label" + } + if(params.rra && params.mle_design_matrix) { warning "mle_design_matrix will only be used for the MAGeCK MLE computations" } @@ -100,6 +104,7 @@ include { MAGECK_GRAPHRRA } from '../modules/local/mageck/grap include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' include { CRISPRCLEANR_NORMALIZE } from '../modules/nf-core/crisprcleanr/normalize/main' include { MAGECK_MLE as MAGECK_MLE_MATRIX } from '../modules/nf-core/mageck/mle/main' +include { MAGECK_MLE as MAGECK_MLE_DAY0 } from '../modules/nf-core/mageck/mle/main' include { BOWTIE2_BUILD } from '../modules/nf-core/bowtie2/build/main' include { BOWTIE2_ALIGN } from '../modules/nf-core/bowtie2/align/main' @@ -324,7 +329,7 @@ workflow CRISPRSEQ_SCREENING { } - if((params.mle_design_matrix) || (params.contrasts && !params.rra)) { + if((params.mle_design_matrix) || (params.contrasts && !params.rra) || (params.day0_label)) { if(params.mle_design_matrix) { ch_design.map { it -> [[id: it.getBaseName()], it] @@ -332,7 +337,6 @@ workflow CRISPRSEQ_SCREENING { ch_mle = ch_designed_mle.combine(ch_counts) MAGECK_MLE_MATRIX (ch_mle) MAGECK_FLUTEMLE(MAGECK_MLE.out.gene_summary) - } if(params.contrasts) { MATRICESCREATION(ch_contrasts) @@ -341,6 +345,12 @@ workflow CRISPRSEQ_SCREENING { ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) MAGECK_FLUTEMLE(MAGECK_MLE.out.gene_summary) } + if(params.day0_label) { + ch_mle = Channel.of([id: "day0"]).merge(Channel.of([[]])).merge(ch_counts) + MAGECK_MLE_DAY0 (ch_mle) + ch_versions = ch_versions.mix(MAGECK_MLE_DAY0.out.versions) + MAGECK_FLUTEMLE(MAGECK_MLE_DAY0.out.gene_summary) + } } CUSTOM_DUMPSOFTWAREVERSIONS ( From c66c1ef564e06c89b1ff32dba7047d334a68cfaf Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Fri, 15 Mar 2024 16:51:05 +0100 Subject: [PATCH 080/226] add fluteMLE --- modules/local/mageck/flutemle.nf | 13 +++++++++++-- 1 file changed, 11 insertions(+), 2 deletions(-) diff --git a/modules/local/mageck/flutemle.nf b/modules/local/mageck/flutemle.nf index 06416fca..c45e53c3 100644 --- a/modules/local/mageck/flutemle.nf +++ b/modules/local/mageck/flutemle.nf @@ -35,11 +35,20 @@ process MAGECK_FLUTEMLE { library(clusterProfiler) library(ggplot2) - #library(pathview) + library(pathview) options(ggrepel.max.overlaps = Inf) mle <- read.table("${gene_summary}", header = TRUE, sep = "\t", stringsAsFactors = FALSE) - print(head(mle)) + #print(head(mle)) #stop(colnames(mle)) + + if("${prefix}" == "day0") { + print("true") + beta_strings <- grep("$\\.beta", colnames(mle), value = TRUE) + before_beta <- sub("$\\.beta.*", "", beta_strings) + unique_strings <- unique(before_beta) + print(unique_strings) + print(column_names) + } FluteMLE(mle, treatname= "${prefix}", proj="${prefix}", pathview.top=0, $args) version_file_path <- "versions.yml" From 4aa4ffa36f24fa4b8c87c5dac1beed413c917ae7 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 18 Mar 2024 09:16:42 +0100 Subject: [PATCH 081/226] Adding day 0 and fluteMLE --- conf/modules.config | 9 +++++++++ modules/nf-core/mageck/mle/main.nf | 7 +++++-- nextflow.config | 1 + nextflow_schema.json | 4 ++++ workflows/crisprseq_screening.nf | 14 ++++++++++++-- 5 files changed, 31 insertions(+), 4 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index bfbfc2fd..bdde0e9d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -130,6 +130,15 @@ process { containerOptions = '' } + withName: MAGECK_MLE_DAY0 { + ext.args = "--day0-label '${params.day0_label}'" + publishDir = [ + path: { "${params.outdir}/mageck/mle/day0/" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + withName: CRISPRCLEANR_NORMALIZE { publishDir = [ path: { "${params.outdir}/crisprcleanr/normalization/" }, diff --git a/modules/nf-core/mageck/mle/main.nf b/modules/nf-core/mageck/mle/main.nf index a08a0431..2b401de3 100644 --- a/modules/nf-core/mageck/mle/main.nf +++ b/modules/nf-core/mageck/mle/main.nf @@ -21,6 +21,8 @@ process MAGECK_MLE { script: def args = task.ext.args ?: '' prefix = meta.id ?: "${meta.treatment}_vs_${meta.reference}" + def design_command = design_matrix ? "-d $design_matrix" : '' + prefix = meta.id ?: "${meta.treatment}_vs_${meta.treatment}" """ @@ -29,8 +31,9 @@ process MAGECK_MLE { $args \\ --threads $task.cpus \\ -k $count_table \\ - -d $design_matrix \\ - -n $prefix + -n $prefix \\ + $design_command + cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/nextflow.config b/nextflow.config index 4ca1a12e..ea092b2d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -21,6 +21,7 @@ params { count_table = null fasta = null five_prime_adapter = null + day0_label = null three_prime_adapter = null min_reads = 30 min_targeted_genes = 3 diff --git a/nextflow_schema.json b/nextflow_schema.json index 09140482..f2034403 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -171,6 +171,10 @@ "type": "string", "description": "Fasta file in case you want to map with bowtie2 and then MAGeCK count" }, + "day0_label": { + "type": "string", + "description": "Specify the label for control sample (usually day 0 or plasmid). For every other sample label, the module will treat it as a treatment condition and compare with control sample for MAGeCK MLE" + }, "mle_design_matrix": { "type": "string", "format": "file-path", diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 4f379f97..b47b8a17 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -42,6 +42,10 @@ if(params.fasta && !params.library) { error "Please provide a fasta file and the library file" } +if(params.day0_label && params.mle_design_matrix) { + warning "MAGeCK MLE module will be ran twice, once with the design matrices and once with day0-label" + } + if(params.rra && params.mle_design_matrix) { warning "mle_design_matrix will only be used for the MAGeCK MLE computations" } @@ -100,6 +104,7 @@ include { MAGECK_GRAPHRRA } from '../modules/local/mageck/grap include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' include { CRISPRCLEANR_NORMALIZE } from '../modules/nf-core/crisprcleanr/normalize/main' include { MAGECK_MLE as MAGECK_MLE_MATRIX } from '../modules/nf-core/mageck/mle/main' +include { MAGECK_MLE as MAGECK_MLE_DAY0 } from '../modules/nf-core/mageck/mle/main' include { BOWTIE2_BUILD } from '../modules/nf-core/bowtie2/build/main' include { BOWTIE2_ALIGN } from '../modules/nf-core/bowtie2/align/main' @@ -328,7 +333,7 @@ workflow CRISPRSEQ_SCREENING { } - if((params.mle_design_matrix) || (params.contrasts && !params.rra)) { + if((params.mle_design_matrix) || (params.contrasts && !params.rra) || (params.day0_label)) { if(params.mle_design_matrix) { ch_design.map { it -> [[id: it.getBaseName()], it] @@ -336,7 +341,6 @@ workflow CRISPRSEQ_SCREENING { ch_mle = ch_designed_mle.combine(ch_counts) MAGECK_MLE_MATRIX (ch_mle) MAGECK_FLUTEMLE(MAGECK_MLE.out.gene_summary) - } if(params.contrasts) { MATRICESCREATION(ch_contrasts) @@ -345,6 +349,12 @@ workflow CRISPRSEQ_SCREENING { ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) MAGECK_FLUTEMLE(MAGECK_MLE.out.gene_summary) } + if(params.day0_label) { + ch_mle = Channel.of([id: "day0"]).merge(Channel.of([[]])).merge(ch_counts) + MAGECK_MLE_DAY0 (ch_mle) + ch_versions = ch_versions.mix(MAGECK_MLE_DAY0.out.versions) + MAGECK_FLUTEMLE(MAGECK_MLE_DAY0.out.gene_summary) + } } CUSTOM_DUMPSOFTWAREVERSIONS ( From 1fee89af957218c40ca9ec94ed5f300351cd7473 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Mon, 18 Mar 2024 13:25:33 +0100 Subject: [PATCH 082/226] Add day0 in fluteMLE --- modules/local/mageck/flutemle.nf | 49 ++++---------------------------- templates/template_fluteMLE.R | 41 ++++++++++++++++++++++++++ 2 files changed, 46 insertions(+), 44 deletions(-) create mode 100644 templates/template_fluteMLE.R diff --git a/modules/local/mageck/flutemle.nf b/modules/local/mageck/flutemle.nf index c45e53c3..59f3346f 100644 --- a/modules/local/mageck/flutemle.nf +++ b/modules/local/mageck/flutemle.nf @@ -13,54 +13,15 @@ process MAGECK_FLUTEMLE { output: tuple val(meta), path("MAGeCKFlute_*/Enrichment/*"), emit: enrich - tuple val(meta), path("MAGeCKFlute_*/QC/*"), emit: qc - tuple val(meta), path("MAGeCKFlute_*/Selection/*"), emit: select - path "versions.yml" , emit: versions + tuple val(meta), path("MAGeCKFlute_*/QC/*"), emit: qc + tuple val(meta), path("MAGeCKFlute_*/Selection/*"), emit: select + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' + args = task.ext.args ?: ' ' prefix = meta.id ?: "${meta.treatment}_vs_${meta.reference}" - - """ - #!/usr/bin/env Rscript - #### author: Laurence Kuhlburger - #### Released under the MIT license. See git repository (https://github.com/nf-core/crisprseq) for full license text. - #### - #### graphs mageck MLE - - library(MAGeCKFlute) - library(clusterProfiler) - library(ggplot2) - - library(pathview) - options(ggrepel.max.overlaps = Inf) - mle <- read.table("${gene_summary}", header = TRUE, sep = "\t", stringsAsFactors = FALSE) - #print(head(mle)) - #stop(colnames(mle)) - - if("${prefix}" == "day0") { - print("true") - beta_strings <- grep("$\\.beta", colnames(mle), value = TRUE) - before_beta <- sub("$\\.beta.*", "", beta_strings) - unique_strings <- unique(before_beta) - print(unique_strings) - print(column_names) - } - FluteMLE(mle, treatname= "${prefix}", proj="${prefix}", pathview.top=0, $args) - - version_file_path <- "versions.yml" - version_flute <- paste(unlist(packageVersion("MAGeCKFlute")), collapse = ".") - version_ggplot <- paste(unlist(packageVersion("ggplot2")), collapse = ".") - - f <- file(version_file_path, "w") - writeLines('"${task.process}":', f) - writeLines(" MAGeCKFlute: ", f, sep = "") - writeLines(version_flute, f) - writeLines(" ggplot2: ", f, sep = "") - writeLines(version_ggplot, f) - close(f) - """ + template 'template_fluteMLE.R' } diff --git a/templates/template_fluteMLE.R b/templates/template_fluteMLE.R new file mode 100644 index 00000000..ab680af0 --- /dev/null +++ b/templates/template_fluteMLE.R @@ -0,0 +1,41 @@ + #!/usr/bin/env Rscript + #### author: Laurence Kuhlburger + #### Released under the MIT license. See git repository (https://github.com/nf-core/crisprseq) for full license text. + #### + #### graphs mageck MLE + + library(MAGeCKFlute) + library(clusterProfiler) + library(ggplot2) + + library(pathview) + options(ggrepel.max.overlaps = Inf) + mle <- read.table("${gene_summary}", header = TRUE, sep = "\t", stringsAsFactors = FALSE) + #print(head(mle)) + #stop(colnames(mle)) + + if("${prefix}" == "day0") { + print("true") + beta_strings <- grep("\\\\.beta", colnames(mle), value = TRUE) + before_beta <- sub("\\\\.beta.*", "", beta_strings) + unique_strings <- unique(before_beta) + print(unique_strings) + for(i in unique_strings) { + FluteMLE(mle, treatname= i, proj="${prefix}", pathview.top=0) + } + #print(column_names) + } else { + FluteMLE(mle, treatname= "${prefix}", proj="${prefix}", ${args}, pathview.top=0) + } + + version_file_path <- "versions.yml" + version_flute <- paste(unlist(packageVersion("MAGeCKFlute")), collapse = ".") + version_ggplot <- paste(unlist(packageVersion("ggplot2")), collapse = ".") + + f <- file(version_file_path, "w") + writeLines('"${task.process}":', f) + writeLines(" MAGeCKFlute: ", f, sep = "") + writeLines(version_flute, f) + writeLines(" ggplot2: ", f, sep = "") + writeLines(version_ggplot, f) + close(f) From 2e87fd46d995e843e253dc11db3b6fa2a9a7d233 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Mon, 18 Mar 2024 13:41:32 +0100 Subject: [PATCH 083/226] Fix typo in fluteMLE --- templates/template_fluteMLE.R | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/templates/template_fluteMLE.R b/templates/template_fluteMLE.R index ab680af0..59bd3822 100644 --- a/templates/template_fluteMLE.R +++ b/templates/template_fluteMLE.R @@ -21,7 +21,7 @@ unique_strings <- unique(before_beta) print(unique_strings) for(i in unique_strings) { - FluteMLE(mle, treatname= i, proj="${prefix}", pathview.top=0) + FluteMLE(mle, treatname= i, proj=i, pathview.top=0) } #print(column_names) } else { From e2c55015eb2d0368cef26a2e26de0b7e375d682c Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Mon, 18 Mar 2024 13:50:09 +0100 Subject: [PATCH 084/226] Remove prints --- templates/template_fluteMLE.R | 3 --- 1 file changed, 3 deletions(-) diff --git a/templates/template_fluteMLE.R b/templates/template_fluteMLE.R index 59bd3822..de5ef71c 100644 --- a/templates/template_fluteMLE.R +++ b/templates/template_fluteMLE.R @@ -15,15 +15,12 @@ #stop(colnames(mle)) if("${prefix}" == "day0") { - print("true") beta_strings <- grep("\\\\.beta", colnames(mle), value = TRUE) before_beta <- sub("\\\\.beta.*", "", beta_strings) unique_strings <- unique(before_beta) - print(unique_strings) for(i in unique_strings) { FluteMLE(mle, treatname= i, proj=i, pathview.top=0) } - #print(column_names) } else { FluteMLE(mle, treatname= "${prefix}", proj="${prefix}", ${args}, pathview.top=0) } From 81c83e4634a97954829080921e1c7ccc69158f38 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 20 Mar 2024 14:09:36 +0100 Subject: [PATCH 085/226] Fix day0 and fluteMLE --- modules/local/mageckflute.nf | 91 ++++++++++++++++++++++++++++++++++++ 1 file changed, 91 insertions(+) create mode 100644 modules/local/mageckflute.nf diff --git a/modules/local/mageckflute.nf b/modules/local/mageckflute.nf new file mode 100644 index 00000000..bf1c685f --- /dev/null +++ b/modules/local/mageckflute.nf @@ -0,0 +1,91 @@ +// TODO nf-core: If in doubt look at other nf-core/modules to see how we are doing things! :) +// https://github.com/nf-core/modules/tree/master/modules/nf-core/ +// You can also ask for help via your pull request or on the #modules channel on the nf-core Slack workspace: +// https://nf-co.re/join +// TODO nf-core: A module file SHOULD only define input and output files as command-line parameters. +// All other parameters MUST be provided using the "task.ext" directive, see here: +// https://www.nextflow.io/docs/latest/process.html#ext +// where "task.ext" is a string. +// Any parameters that need to be evaluated in the context of a particular sample +// e.g. single-end/paired-end data MUST also be defined and evaluated appropriately. +// TODO nf-core: Software that can be piped together SHOULD be added to separate module files +// unless there is a run-time, storage advantage in implementing in this way +// e.g. it's ok to have a single module for bwa to output BAM instead of SAM: +// bwa mem | samtools view -B -T ref.fasta +// TODO nf-core: Optional inputs are not currently supported by Nextflow. However, using an empty +// list (`[]`) instead of a file can be used to work around this issue. + +process MAGECKFLUTE { + tag "$meta.id" + label 'process_single' + + // TODO nf-core: List required Conda package(s). + // Software MUST be pinned to channel (i.e. "bioconda"), version (i.e. "1.10"). + // For Conda, the build (i.e. "h9402c20_2") must be EXCLUDED to support installation on different operating systems. + // TODO nf-core: See section in main README for further information regarding finding and adding container addresses to the section below. + conda "bioconda::bioconductor-mageckflute=2.6.0" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/YOUR-TOOL-HERE': + 'biocontainers/YOUR-TOOL-HERE' }" + + input: + // TODO nf-core: Where applicable all sample-specific information e.g. "id", "single_end", "read_group" + // MUST be provided as an input via a Groovy Map called "meta". + // This information may not be required in some instances e.g. indexing reference genome files: + // https://github.com/nf-core/modules/blob/master/modules/nf-core/bwa/index/main.nf + // TODO nf-core: Where applicable please provide/convert compressed files as input/output + // e.g. "*.fastq.gz" and NOT "*.fastq", "*.bam" and NOT "*.sam" etc. + tuple val(meta), path(bam) + + output: + // TODO nf-core: Named file extensions MUST be emitted for ALL output channels + tuple val(meta), path("*.bam"), emit: bam + // TODO nf-core: List additional required output channels/values here + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + // TODO nf-core: Where possible, a command MUST be provided to obtain the version number of the software e.g. 1.10 + // If the software is unable to output a version number on the command-line then it can be manually specified + // e.g. https://github.com/nf-core/modules/blob/master/modules/nf-core/homer/annotatepeaks/main.nf + // Each software used MUST provide the software name and version number in the YAML version file (versions.yml) + // TODO nf-core: It MUST be possible to pass additional parameters to the tool as a command-line string via the "task.ext.args" directive + // TODO nf-core: If the tool supports multi-threading then you MUST provide the appropriate parameter + // using the Nextflow "task" variable e.g. "--threads $task.cpus" + // TODO nf-core: Please replace the example samtools command below with your module's command + // TODO nf-core: Please indent the command appropriately (4 spaces!!) to help with readability ;) + """ + samtools \\ + sort \\ + $args \\ + -@ $task.cpus \\ + -o ${prefix}.bam \\ + -T $prefix \\ + $bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + : \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' )) + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + // TODO nf-core: A stub section should mimic the execution of the original module as best as possible + // Have a look at the following examples: + // Simple example: https://github.com/nf-core/modules/blob/818474a292b4860ae8ff88e149fbcda68814114d/modules/nf-core/bcftools/annotate/main.nf#L47-L63 + // Complex example: https://github.com/nf-core/modules/blob/818474a292b4860ae8ff88e149fbcda68814114d/modules/nf-core/bedtools/split/main.nf#L38-L54 + """ + touch ${prefix}.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + : \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' )) + END_VERSIONS + """ +} From 137b1af9b9b85b2d57837ce7b01ed4773c9f2ecd Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 20 Mar 2024 15:05:43 +0100 Subject: [PATCH 086/226] Add day0 label, fix mageck MLE and add fluteMLE --- CHANGELOG.md | 4 +- CITATIONS.md | 3 + conf/modules.config | 5 ++ docs/output/screening.md | 17 ++++ docs/usage/screening.md | 16 ++-- modules.json | 90 ++++++++++++++++------ modules/local/mageck/flutemle.nf | 1 + modules/nf-core/mageck/mle/mageck-mle.diff | 22 +++++- modules/nf-core/mageck/mle/main.nf | 4 +- templates/template_fluteMLE.R | 6 +- 10 files changed, 126 insertions(+), 42 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 794ea696..198a28a8 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,8 +10,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Template update to 2.11.1 ([#105](https://github.com/nf-core/crisprseq/pull/105)) - Added a csv input option for crisprcleanr ([#105](https://github.com/nf-core/crisprseq/pull/105)) - Added a contrasts parameter so the pipeline automatically creates design matrices and MAGeCK MLE ([#109](https://github.com/nf-core/crisprseq/pull/109)) -- Added bowtie2 and three prime and five prime adapter trimming ([#103](https://github.com/nf-core/crisprseq/pull/103) and [#123](https://github.com/nf-core/crisprseq/pull/123) - ) +- Added bowtie2 and three prime and five prime adapter trimming ([#103](https://github.com/nf-core/crisprseq/pull/103) and [#123](https://github.com/nf-core/crisprseq/pull/123)) +- Added --day0_label and FluteMLE for MLE data [#126](https://github.com/nf-core/crisprseq/pull/126) ### Fixed diff --git a/CITATIONS.md b/CITATIONS.md index 6e78897d..3b2e1e90 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -45,6 +45,9 @@ - [Samtools](10.1093/bioinformatics/btp352) > Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. doi: 10.1093/bioinformatics/btp352. Epub 2009 Jun 8. PMID: 19505943; PMCID: PMC2723002. +- [MAGeCKFlute](https://doi.org/10.1038/s41596-018-0113-7) + > Wang, B., Wang, M., Zhang, W. et al. Integrative analysis of pooled CRISPR genetic screens using MAGeCKFlute. Nat Protoc 14, 756–780 (2019). + ## Software packaging/containerisation tools - [Anaconda](https://anaconda.com) diff --git a/conf/modules.config b/conf/modules.config index 46e51e67..f644a82e 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -128,6 +128,11 @@ process { withName: MAGECK_FLUTEMLE { containerOptions = '' + publishDir = [ + path: { "${params.outdir}/mageck/FluteMLE/" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } withName: MAGECK_MLE_DAY0 { diff --git a/docs/output/screening.md b/docs/output/screening.md index 27bbf16f..e964991b 100644 --- a/docs/output/screening.md +++ b/docs/output/screening.md @@ -148,6 +148,23 @@ For further reading and documentation see the [cutadapt helper page](https://cut [bagel2](https://github.com/hart-lab/bagel) is a computational tool to identify important essential genes for CRISPR-Cas9 screening experiments. +## Gene essentiality computation + +### MAGeCKFlute + +- `FluteMLE/QC` + - `*.txt` : Quality control tables + - `*.png` : Quality control plots +- `FluteMLE/Selection` + - `*.txt`: Positive selection and negative selection. + - `*.png`: Rank and scatter view for positive and negative selection +- `FluteMLE/Enrichment` + - `*.txt`: Enrichment analysis for positive and negative selection genes. + - `*.png`: Enrichment analysis plots for positive and negative selection genes. +- `FluteMLE/PathwayView` + - `*.txt`: Pathway view for top enriched pathways. + - `*.png`: Pathway view for top enriched pathways. + ## MultiQC
    diff --git a/docs/usage/screening.md b/docs/usage/screening.md index 68bc145d..e8e99500 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -66,13 +66,11 @@ CRISPRcleanR is used for gene count normalization and the removal of biases for Most used library already have an annotation dataset which you can find [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). Otherwise, if you wish to provide your own file, please provide it in csv form, and make sure it follows the following format : -| ,CODE | GENES | EXONE | CHRM | STRAND | STARTpos | ENDpos | -| -------------------- | -------------- | ------- | ---- | ------ | -------- | --------- | --------- | -| AAAAAAAAAAAATGCATTCT | NM_183035.1 | Defb34 | ex2 | 8 | - | 19126349 | 19126369 | -| AAAAAAAAATAAGCTCACCC | NM_001170853.1 | Mndal | ex5 | 1 | + | 173872968 | 173872988 | -| AAAAAAAATCCTGTCGCCCA | NM_001039049.1 | Cox8c | ex1 | 12 | + | 102899487 | 102899507 | -| AAAAAAATCGGCATACCATG | NM_178627.3 | Poldip3 | ex4 | 15 | - | 83135295 | 83135315 | -| AAAAAAATGACATTACTGCA | NM_026602.3 | Bcas2 | ex4 | 3 | + | 103174386 | 103174406 | +| ,CODE | GENES | EXONE | CHRM | STRAND | STARTpos | ENDpos | +|---------------|-------------|-------------|------|--------|----------|----------| +| ATGGTGTCCATTATAGCCAT | NM_021446.2 | 0610007P14Rik | ex2 | 12 | + | 85822165 | 85822185 | +| CTCTACGAGAAGCTCTACAC | NM_021446.2 | 0610007P14Rik | ex2 | 12 | + | 85822108 | 85822128 | +| GACTCTATCACATCACACTG | NM_021446.2 | 0610007P14Rik | ex4 | 12 | + | 85816419 | 85816439 | ### Running MAGeCK MLE and BAGEL2 with a contrast file @@ -102,6 +100,10 @@ If there are several designs to be run, you can input a folder containing all th BAGEL2 (Bayesian Analysis of Gene Essentiality with Location) is a computational tool developed by the Hart Lab at Harvard University. It is designed for analyzing large-scale genetic screens, particularly CRISPR-Cas9 screens, to identify genes that are essential for the survival or growth of cells under different conditions. BAGEL2 integrates information about the location of guide RNAs within a gene and leverages this information to improve the accuracy of gene essentiality predictions. BAGEL2 uses the same contrasts from `--contrasts`. +### MAGECKFlute + +The downstream analysis involves distinguishing essential, non-essential, and target-associated genes. Additionally, it encompasses conducting biological functional category analysis and pathway enrichment analysis for these genes. Furthermore, the function provides visualization of genes within pathways, enhancing user exploration of screening data. MAGECKFlute is run automatically after MAGeCK MLE and for each MLE design matrice. If you have used the `--day0_label`, MAGeCKFlute will be ran on all the other conditions. Please note that the DepMap data is used for these plots. + Note that the pipeline will create the following files in your working directory: ```bash diff --git a/modules.json b/modules.json index b7928c26..44d4fbc9 100644 --- a/modules.json +++ b/modules.json @@ -8,120 +8,164 @@ "bowtie2/align": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bowtie2/build": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/mem": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cat/fastq": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cutadapt": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/cutadapt/cutadapt.diff" }, "fastqc": { "branch": "master", "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "mageck/count": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/count/mageck-count.diff" }, "mageck/mle": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" }, "mageck/test": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/test/mageck-test.diff" }, "medaka": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/medaka/medaka.diff" }, "minimap2/align": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" }, "minimap2/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "multiqc": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "pear": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "racon": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/index": { "branch": "master", "git_sha": "5394565c5fe4c760e5b35977ec7607c62e81d1f8", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "seqtk/seq": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "vsearch/cluster": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" }, "vsearch/sort": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] } } }, @@ -130,4 +174,4 @@ } } } -} +} \ No newline at end of file diff --git a/modules/local/mageck/flutemle.nf b/modules/local/mageck/flutemle.nf index 59f3346f..5ee890fd 100644 --- a/modules/local/mageck/flutemle.nf +++ b/modules/local/mageck/flutemle.nf @@ -15,6 +15,7 @@ process MAGECK_FLUTEMLE { tuple val(meta), path("MAGeCKFlute_*/Enrichment/*"), emit: enrich tuple val(meta), path("MAGeCKFlute_*/QC/*"), emit: qc tuple val(meta), path("MAGeCKFlute_*/Selection/*"), emit: select + tuple val(meta), path("MAGeCKFlute_*/PathwayView/*"), emit: pathwayview path "versions.yml" , emit: versions when: diff --git a/modules/nf-core/mageck/mle/mageck-mle.diff b/modules/nf-core/mageck/mle/mageck-mle.diff index e7b6f9ec..d8a988f1 100644 --- a/modules/nf-core/mageck/mle/mageck-mle.diff +++ b/modules/nf-core/mageck/mle/mageck-mle.diff @@ -1,7 +1,7 @@ Changes in module 'nf-core/mageck/mle' --- modules/nf-core/mageck/mle/main.nf +++ modules/nf-core/mageck/mle/main.nf -@@ -1,6 +1,6 @@ +@@ -1,15 +1,14 @@ process MAGECK_MLE { - tag "$meta.id" - label 'process_medium' @@ -10,8 +10,10 @@ Changes in module 'nf-core/mageck/mle' conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? -@@ -8,8 +8,7 @@ - 'biocontainers/mageck:0.5.9--py37h6bb024c_0' }" +- 'https://depot.galaxyproject.org/singularity/mageck:0.5.9--py37h6bb024c_0': +- 'biocontainers/mageck:0.5.9--py37h6bb024c_0' }" ++ 'https://depot.galaxyproject.org/singularity/mageck:0.5.9.5--py39h1f90b4d_3': ++ 'biocontainers/mageck:0.5.9.5--py39h1f90b4d_3' }" input: - tuple val(meta), path(count_table) @@ -26,9 +28,21 @@ Changes in module 'nf-core/mageck/mle' def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" + prefix = meta.id ?: "${meta.treatment}_vs_${meta.reference}" -+ ++ def design_command = design_matrix ? "-d $design_matrix" : '' """ mageck \\ +@@ -29,8 +29,9 @@ + $args \\ + --threads $task.cpus \\ + -k $count_table \\ +- -d $design_matrix \\ +- -n $prefix ++ -n $prefix \\ ++ $design_command ++ + + cat <<-END_VERSIONS > versions.yml + "${task.process}": ************************************************************ diff --git a/modules/nf-core/mageck/mle/main.nf b/modules/nf-core/mageck/mle/main.nf index 82e6cb61..049254a3 100644 --- a/modules/nf-core/mageck/mle/main.nf +++ b/modules/nf-core/mageck/mle/main.nf @@ -4,8 +4,8 @@ process MAGECK_MLE { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mageck:0.5.9--py37h6bb024c_0': - 'biocontainers/mageck:0.5.9--py37h6bb024c_0' }" + 'https://depot.galaxyproject.org/singularity/mageck:0.5.9.5--py39h1f90b4d_3': + 'biocontainers/mageck:0.5.9.5--py39h1f90b4d_3' }" input: tuple val(meta), path(design_matrix), path(count_table) diff --git a/templates/template_fluteMLE.R b/templates/template_fluteMLE.R index de5ef71c..e30c63bd 100644 --- a/templates/template_fluteMLE.R +++ b/templates/template_fluteMLE.R @@ -11,18 +11,16 @@ library(pathview) options(ggrepel.max.overlaps = Inf) mle <- read.table("${gene_summary}", header = TRUE, sep = "\t", stringsAsFactors = FALSE) - #print(head(mle)) - #stop(colnames(mle)) if("${prefix}" == "day0") { beta_strings <- grep("\\\\.beta", colnames(mle), value = TRUE) before_beta <- sub("\\\\.beta.*", "", beta_strings) unique_strings <- unique(before_beta) for(i in unique_strings) { - FluteMLE(mle, treatname= i, proj=i, pathview.top=0) + FluteMLE(mle, treatname= i, proj=i, pathview.top=5) } } else { - FluteMLE(mle, treatname= "${prefix}", proj="${prefix}", ${args}, pathview.top=0) + FluteMLE(mle, treatname= "${prefix}", proj="${prefix}", ${args}, pathview.top=5) } version_file_path <- "versions.yml" From ce0ea5190f517f39089226986f7c6ee7c2cd5978 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 20 Mar 2024 15:34:58 +0100 Subject: [PATCH 087/226] run pre commit and prettier --- CITATIONS.md | 1 + docs/usage/screening.md | 4 +- modules.json | 90 +++++++++++------------------------------ 3 files changed, 26 insertions(+), 69 deletions(-) diff --git a/CITATIONS.md b/CITATIONS.md index 3b2e1e90..887c4621 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -43,6 +43,7 @@ > Martin, M. (2011). Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet.journal, 17(1), pp. 10-12. doi:https://doi.org/10.14806/ej.17.1.200 - [Samtools](10.1093/bioinformatics/btp352) + > Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. doi: 10.1093/bioinformatics/btp352. Epub 2009 Jun 8. PMID: 19505943; PMCID: PMC2723002. - [MAGeCKFlute](https://doi.org/10.1038/s41596-018-0113-7) diff --git a/docs/usage/screening.md b/docs/usage/screening.md index e8e99500..f7781d4f 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -66,8 +66,8 @@ CRISPRcleanR is used for gene count normalization and the removal of biases for Most used library already have an annotation dataset which you can find [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). Otherwise, if you wish to provide your own file, please provide it in csv form, and make sure it follows the following format : -| ,CODE | GENES | EXONE | CHRM | STRAND | STARTpos | ENDpos | -|---------------|-------------|-------------|------|--------|----------|----------| +| ,CODE | GENES | EXONE | CHRM | STRAND | STARTpos | ENDpos | +| -------------------- | ----------- | ------------- | ---- | ------ | -------- | -------- | -------- | | ATGGTGTCCATTATAGCCAT | NM_021446.2 | 0610007P14Rik | ex2 | 12 | + | 85822165 | 85822185 | | CTCTACGAGAAGCTCTACAC | NM_021446.2 | 0610007P14Rik | ex2 | 12 | + | 85822108 | 85822128 | | GACTCTATCACATCACACTG | NM_021446.2 | 0610007P14Rik | ex4 | 12 | + | 85816419 | 85816439 | diff --git a/modules.json b/modules.json index 44d4fbc9..b7928c26 100644 --- a/modules.json +++ b/modules.json @@ -8,164 +8,120 @@ "bowtie2/align": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bowtie2/build": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cat/fastq": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cutadapt": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/cutadapt/cutadapt.diff" }, "fastqc": { "branch": "master", "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "mageck/count": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/count/mageck-count.diff" }, "mageck/mle": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" }, "mageck/test": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/test/mageck-test.diff" }, "medaka": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/medaka/medaka.diff" }, "minimap2/align": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" }, "minimap2/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "multiqc": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "pear": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "racon": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/index": { "branch": "master", "git_sha": "5394565c5fe4c760e5b35977ec7607c62e81d1f8", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "seqtk/seq": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "vsearch/cluster": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" }, "vsearch/sort": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } }, @@ -174,4 +130,4 @@ } } } -} \ No newline at end of file +} From 5549383f77377e9957a7b8e6b08d7d483f37c2b0 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Thu, 21 Mar 2024 08:26:50 +0100 Subject: [PATCH 088/226] Delete modules/local/mageckflute.nf --- modules/local/mageckflute.nf | 91 ------------------------------------ 1 file changed, 91 deletions(-) delete mode 100644 modules/local/mageckflute.nf diff --git a/modules/local/mageckflute.nf b/modules/local/mageckflute.nf deleted file mode 100644 index bf1c685f..00000000 --- a/modules/local/mageckflute.nf +++ /dev/null @@ -1,91 +0,0 @@ -// TODO nf-core: If in doubt look at other nf-core/modules to see how we are doing things! :) -// https://github.com/nf-core/modules/tree/master/modules/nf-core/ -// You can also ask for help via your pull request or on the #modules channel on the nf-core Slack workspace: -// https://nf-co.re/join -// TODO nf-core: A module file SHOULD only define input and output files as command-line parameters. -// All other parameters MUST be provided using the "task.ext" directive, see here: -// https://www.nextflow.io/docs/latest/process.html#ext -// where "task.ext" is a string. -// Any parameters that need to be evaluated in the context of a particular sample -// e.g. single-end/paired-end data MUST also be defined and evaluated appropriately. -// TODO nf-core: Software that can be piped together SHOULD be added to separate module files -// unless there is a run-time, storage advantage in implementing in this way -// e.g. it's ok to have a single module for bwa to output BAM instead of SAM: -// bwa mem | samtools view -B -T ref.fasta -// TODO nf-core: Optional inputs are not currently supported by Nextflow. However, using an empty -// list (`[]`) instead of a file can be used to work around this issue. - -process MAGECKFLUTE { - tag "$meta.id" - label 'process_single' - - // TODO nf-core: List required Conda package(s). - // Software MUST be pinned to channel (i.e. "bioconda"), version (i.e. "1.10"). - // For Conda, the build (i.e. "h9402c20_2") must be EXCLUDED to support installation on different operating systems. - // TODO nf-core: See section in main README for further information regarding finding and adding container addresses to the section below. - conda "bioconda::bioconductor-mageckflute=2.6.0" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/YOUR-TOOL-HERE': - 'biocontainers/YOUR-TOOL-HERE' }" - - input: - // TODO nf-core: Where applicable all sample-specific information e.g. "id", "single_end", "read_group" - // MUST be provided as an input via a Groovy Map called "meta". - // This information may not be required in some instances e.g. indexing reference genome files: - // https://github.com/nf-core/modules/blob/master/modules/nf-core/bwa/index/main.nf - // TODO nf-core: Where applicable please provide/convert compressed files as input/output - // e.g. "*.fastq.gz" and NOT "*.fastq", "*.bam" and NOT "*.sam" etc. - tuple val(meta), path(bam) - - output: - // TODO nf-core: Named file extensions MUST be emitted for ALL output channels - tuple val(meta), path("*.bam"), emit: bam - // TODO nf-core: List additional required output channels/values here - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - // TODO nf-core: Where possible, a command MUST be provided to obtain the version number of the software e.g. 1.10 - // If the software is unable to output a version number on the command-line then it can be manually specified - // e.g. https://github.com/nf-core/modules/blob/master/modules/nf-core/homer/annotatepeaks/main.nf - // Each software used MUST provide the software name and version number in the YAML version file (versions.yml) - // TODO nf-core: It MUST be possible to pass additional parameters to the tool as a command-line string via the "task.ext.args" directive - // TODO nf-core: If the tool supports multi-threading then you MUST provide the appropriate parameter - // using the Nextflow "task" variable e.g. "--threads $task.cpus" - // TODO nf-core: Please replace the example samtools command below with your module's command - // TODO nf-core: Please indent the command appropriately (4 spaces!!) to help with readability ;) - """ - samtools \\ - sort \\ - $args \\ - -@ $task.cpus \\ - -o ${prefix}.bam \\ - -T $prefix \\ - $bam - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - : \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' )) - END_VERSIONS - """ - - stub: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - // TODO nf-core: A stub section should mimic the execution of the original module as best as possible - // Have a look at the following examples: - // Simple example: https://github.com/nf-core/modules/blob/818474a292b4860ae8ff88e149fbcda68814114d/modules/nf-core/bcftools/annotate/main.nf#L47-L63 - // Complex example: https://github.com/nf-core/modules/blob/818474a292b4860ae8ff88e149fbcda68814114d/modules/nf-core/bedtools/split/main.nf#L38-L54 - """ - touch ${prefix}.bam - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - : \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' )) - END_VERSIONS - """ -} From 62facd2fc39888ea4579330d6676530f44e02a39 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 21 Mar 2024 09:05:47 +0100 Subject: [PATCH 089/226] finish doc --- docs/usage/screening.md | 6 ++++++ 1 file changed, 6 insertions(+) diff --git a/docs/usage/screening.md b/docs/usage/screening.md index f7781d4f..922cb671 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -89,12 +89,18 @@ MAGeCK RRA performs robust ranking aggregation to identify genes that are consis ### Running MAGeCK MLE only +#### With design matrices + If you wish to run MAGeCK MLE only, you can specify several design matrices (where you state which comparisons you wish to run) with the flag `--mle_design_matrix`. MAGeCK MLE uses a maximum likelihood estimation approach to estimate the effects of gene knockout on cell fitness. It models the read count data of guide RNAs targeting each gene and estimates the dropout probability for each gene. MAGeCK MLE requires one or several design matrices. The design matrix is a `txt` file indicating the effects of different conditions on different samples. An [example design matrix](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/design_matrix.txt) has been provided with the pipeline. The row names need to match the condition stated in the sample sheet. If there are several designs to be run, you can input a folder containing all the design matrices. The output results will automatically take the name of the design matrix, so make sure you give a meaningful name to the file, for instance "Drug_vs_control.txt". +#### With the day0 label + +If you wish to run MAGeCK MLE with the day0 label you can do so by specifying `--day0_label` and the sample names that should be used as day0. + ### Running BAGEL2 BAGEL2 (Bayesian Analysis of Gene Essentiality with Location) is a computational tool developed by the Hart Lab at Harvard University. It is designed for analyzing large-scale genetic screens, particularly CRISPR-Cas9 screens, to identify genes that are essential for the survival or growth of cells under different conditions. BAGEL2 integrates information about the location of guide RNAs within a gene and leverages this information to improve the accuracy of gene essentiality predictions. From 9bab82c84dcb88a18075f95409f6b9efccd336a3 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Thu, 21 Mar 2024 11:22:55 +0100 Subject: [PATCH 090/226] Update workflows/crisprseq_screening.nf MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- workflows/crisprseq_screening.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 1e4d1dd6..19f7c5c7 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -43,7 +43,7 @@ if(params.fasta && !params.library) { } if(params.day0_label && params.mle_design_matrix) { - warning "MAGeCK MLE module will be ran twice, once with the design matrices and once with day0-label" + warning "MAGeCK MLE module will be run twice, once with the design matrix and once with day0-label" } if(params.rra && params.mle_design_matrix) { From fde49d5308d534c739da9bea30857eb1c62ff692 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Thu, 21 Mar 2024 11:23:04 +0100 Subject: [PATCH 091/226] Update CHANGELOG.md MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 198a28a8..f4ddd1d4 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -11,7 +11,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Added a csv input option for crisprcleanr ([#105](https://github.com/nf-core/crisprseq/pull/105)) - Added a contrasts parameter so the pipeline automatically creates design matrices and MAGeCK MLE ([#109](https://github.com/nf-core/crisprseq/pull/109)) - Added bowtie2 and three prime and five prime adapter trimming ([#103](https://github.com/nf-core/crisprseq/pull/103) and [#123](https://github.com/nf-core/crisprseq/pull/123)) -- Added --day0_label and FluteMLE for MLE data [#126](https://github.com/nf-core/crisprseq/pull/126) +- Added `--day0_label` and `FluteMLE` for MLE data [#126](https://github.com/nf-core/crisprseq/pull/126) ### Fixed From 442d5f3473688d64ebbbfed8f718b311ddb64a67 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Thu, 21 Mar 2024 11:23:13 +0100 Subject: [PATCH 092/226] Update conf/modules.config MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- conf/modules.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index f644a82e..a8431a39 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -136,8 +136,8 @@ process { } withName: MAGECK_MLE_DAY0 { - ext.args = "--day0-label '${params.day0_label}'" - publishDir = [ + ext.args = "--day0-label '${params.day0_label}'" + publishDir = [ path: { "${params.outdir}/mageck/mle/day0/" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } From 3498ec094079051a7e2e775a87e7d2c8391a94aa Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 21 Mar 2024 11:29:23 +0100 Subject: [PATCH 093/226] Add version of clusterprofiler --- templates/template_fluteMLE.R | 3 +++ 1 file changed, 3 insertions(+) diff --git a/templates/template_fluteMLE.R b/templates/template_fluteMLE.R index e30c63bd..087aaa09 100644 --- a/templates/template_fluteMLE.R +++ b/templates/template_fluteMLE.R @@ -26,6 +26,7 @@ version_file_path <- "versions.yml" version_flute <- paste(unlist(packageVersion("MAGeCKFlute")), collapse = ".") version_ggplot <- paste(unlist(packageVersion("ggplot2")), collapse = ".") + version_clusterprofiler <- paste(unlist(packageVersion("clusterProfiler")), collapse = ".") f <- file(version_file_path, "w") writeLines('"${task.process}":', f) @@ -33,4 +34,6 @@ writeLines(version_flute, f) writeLines(" ggplot2: ", f, sep = "") writeLines(version_ggplot, f) + writeLines(" clusterProfiler: ", f, sep = "") + writeLines(version_clusterprofiler, f) close(f) From 1858b5e4a28e1a04846caaf826e9db089278a322 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 21 Mar 2024 11:30:51 +0100 Subject: [PATCH 094/226] Add version of clusterprofiler --- templates/template_fluteMLE.R | 3 +++ 1 file changed, 3 insertions(+) diff --git a/templates/template_fluteMLE.R b/templates/template_fluteMLE.R index 087aaa09..b942c898 100644 --- a/templates/template_fluteMLE.R +++ b/templates/template_fluteMLE.R @@ -27,6 +27,7 @@ version_flute <- paste(unlist(packageVersion("MAGeCKFlute")), collapse = ".") version_ggplot <- paste(unlist(packageVersion("ggplot2")), collapse = ".") version_clusterprofiler <- paste(unlist(packageVersion("clusterProfiler")), collapse = ".") + version_pathview <- paste(unlist(packageVersion("pathview")), collapse = ".") f <- file(version_file_path, "w") writeLines('"${task.process}":', f) @@ -36,4 +37,6 @@ writeLines(version_ggplot, f) writeLines(" clusterProfiler: ", f, sep = "") writeLines(version_clusterprofiler, f) + writeLines(" pathview: ", f, sep = "") + writeLines(version_pathview, f) close(f) From ce83b98bd29df83c845054a0b1639c5a6fbcfa57 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Thu, 21 Mar 2024 16:35:15 +0100 Subject: [PATCH 095/226] Apply suggestions from code review - PR testing command Co-authored-by: LaurenceKuhl --- .github/PULL_REQUEST_TEMPLATE.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index c6709972..1ae44009 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/cris - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/crisprseq/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/crisprseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). +- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. From a97ba0d1473d397a2b268d1967ee5a0ca2968b3a Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 21 Mar 2024 19:38:09 +0100 Subject: [PATCH 096/226] Fix bug bc i put the channels at the wrong place --- .nf-core.yml | 2 ++ .../utils_nfcore_crisprseq_pipeline/main.nf | 1 + workflows/crisprseq_screening.nf | 21 +++++++++++++------ 3 files changed, 18 insertions(+), 6 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index 2c087061..bdbcee24 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -4,5 +4,7 @@ lint: # We skip the linting of these files as we have splitted tests between targeted and screening - conf/test.config - conf/test_full.config + # We skip this linting because we have not finished exporting to nf-test + - .github/PULL_REQUEST_TEMPLATE.md files_unchanged: - lib/NfcoreTemplate.groovy # Introduced a change ahead of the nf-core/tools release diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index 2053827a..4929ab09 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -151,6 +151,7 @@ workflow INITIALISATION_CHANNEL_CREATION_SCREENING { ch_design = Channel.fromPath(params.mle_design_matrix) } + emit: library = ch_library // channel: library file crisprcleanr = ch_crisprcleanr // channel: crisprcleanr file or value diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index ada954d3..15940e11 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -224,8 +224,12 @@ workflow CRISPRSEQ_SCREENING { counts = ch_contrasts.combine(ch_counts) //Define non essential and essential genes channels for bagel2 - ch_bagel_reference_essentials= Channel.fromPath(params.bagel_reference_essentials).first() - ch_bagel_reference_nonessentials= Channel.fromPath(params.bagel_reference_nonessentials).first() + if(params.bagel_reference_essentials) { + bagel_reference_essentials= Channel.fromPath(params.bagel_reference_essentials).first() + } + if(params.bagel_reference_nonessentials) { + bagel_reference_nonessentials= Channel.fromPath(params.bagel_reference_nonessentials).first() + } BAGEL2_FC ( counts @@ -234,15 +238,15 @@ workflow CRISPRSEQ_SCREENING { BAGEL2_BF ( BAGEL2_FC.out.foldchange, - ch_bagel_reference_essentials, - ch_bagel_reference_nonessentials + bagel_reference_essentials, + bagel_reference_nonessentials ) ch_versions = ch_versions.mix(BAGEL2_BF.out.versions) - ch_bagel_pr = BAGEL2_BF.out.bf.combine(ch_bagel_reference_essentials) - .combine(ch_bagel_reference_nonessentials) + ch_bagel_pr = BAGEL2_BF.out.bf.combine(bagel_reference_essentials) + .combine(bagel_reference_nonessentials) BAGEL2_PR ( ch_bagel_pr @@ -263,9 +267,12 @@ workflow CRISPRSEQ_SCREENING { INITIALISATION_CHANNEL_CREATION_SCREENING.out.design.map { it -> [[id: it.getBaseName()], it] }.set { ch_designed_mle } + ch_mle = ch_designed_mle.combine(ch_counts) MAGECK_MLE_MATRIX (ch_mle) + ch_versions = ch_versions.mix(MAGECK_MLE_MATRIX.out.versions) MAGECK_FLUTEMLE(MAGECK_MLE.out.gene_summary) + ch_versions = ch_versions.mix(MAGECK_FLUTEMLE.out.versions) } if(params.contrasts) { MATRICESCREATION(ch_contrasts) @@ -273,12 +280,14 @@ workflow CRISPRSEQ_SCREENING { MAGECK_MLE (ch_mle) ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) MAGECK_FLUTEMLE(MAGECK_MLE.out.gene_summary) + ch_versions = ch_versions.mix(MAGECK_FLUTEMLE.out.versions) } if(params.day0_label) { ch_mle = Channel.of([id: "day0"]).merge(Channel.of([[]])).merge(ch_counts) MAGECK_MLE_DAY0 (ch_mle) ch_versions = ch_versions.mix(MAGECK_MLE_DAY0.out.versions) MAGECK_FLUTEMLE(MAGECK_MLE_DAY0.out.gene_summary) + ch_versions = ch_versions.mix(MAGECK_FLUTEMLE.out.versions) } } From 19bdc0ee1a6b77b9b2c79e0fb63e953280cce2f9 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 22 Mar 2024 09:41:03 +0100 Subject: [PATCH 097/226] fix linting --- .nf-core.yml | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index bdbcee24..1b63fd63 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -4,7 +4,6 @@ lint: # We skip the linting of these files as we have splitted tests between targeted and screening - conf/test.config - conf/test_full.config - # We skip this linting because we have not finished exporting to nf-test - - .github/PULL_REQUEST_TEMPLATE.md files_unchanged: - lib/NfcoreTemplate.groovy # Introduced a change ahead of the nf-core/tools release + - .github/PULL_REQUEST_TEMPLATE.md From 01fd057af066904844a85ed4ad23f29dda2a0428 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 22 Mar 2024 09:47:04 +0100 Subject: [PATCH 098/226] fix bagel essential channels --- workflows/crisprseq_screening.nf | 18 +++++++----------- 1 file changed, 7 insertions(+), 11 deletions(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 15940e11..e2ad8cb1 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -223,13 +223,9 @@ workflow CRISPRSEQ_SCREENING { .set { ch_contrasts } counts = ch_contrasts.combine(ch_counts) - //Define non essential and essential genes channels for bagel2 - if(params.bagel_reference_essentials) { - bagel_reference_essentials= Channel.fromPath(params.bagel_reference_essentials).first() - } - if(params.bagel_reference_nonessentials) { - bagel_reference_nonessentials= Channel.fromPath(params.bagel_reference_nonessentials).first() - } + //Define non essential and essential genes channels for bagel2 + ch_bagel_reference_essentials= Channel.fromPath(params.bagel_reference_essentials).first() + ch_bagel_reference_nonessentials= Channel.fromPath(params.bagel_reference_nonessentials).first() BAGEL2_FC ( counts @@ -238,15 +234,15 @@ workflow CRISPRSEQ_SCREENING { BAGEL2_BF ( BAGEL2_FC.out.foldchange, - bagel_reference_essentials, - bagel_reference_nonessentials + ch_bagel_reference_essentials, + ch_bagel_reference_nonessentials ) ch_versions = ch_versions.mix(BAGEL2_BF.out.versions) - ch_bagel_pr = BAGEL2_BF.out.bf.combine(bagel_reference_essentials) - .combine(bagel_reference_nonessentials) + ch_bagel_pr = BAGEL2_BF.out.bf.combine(ch_bagel_reference_essentials) + .combine(ch_bagel_reference_nonessentials) BAGEL2_PR ( ch_bagel_pr From 298be8963a240ccbddbeda17505f34bad8657895 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 22 Mar 2024 09:47:50 +0100 Subject: [PATCH 099/226] fix space --- workflows/crisprseq_screening.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index e2ad8cb1..a78bf732 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -223,7 +223,7 @@ workflow CRISPRSEQ_SCREENING { .set { ch_contrasts } counts = ch_contrasts.combine(ch_counts) - //Define non essential and essential genes channels for bagel2 + //Define non essential and essential genes channels for bagel2 ch_bagel_reference_essentials= Channel.fromPath(params.bagel_reference_essentials).first() ch_bagel_reference_nonessentials= Channel.fromPath(params.bagel_reference_nonessentials).first() From 73811a9c8a805b3e937ae990090c5d6e88ac4ff6 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 27 Mar 2024 10:36:01 +0100 Subject: [PATCH 100/226] First try for a venn diagram --- modules/local/bagel2/bf.nf | 2 +- modules/local/bagel2/graph.nf | 2 +- modules/local/bagel2/pr.nf | 2 +- workflows/crisprseq_screening.nf | 45 ++++++++++---------------------- 4 files changed, 17 insertions(+), 34 deletions(-) diff --git a/modules/local/bagel2/bf.nf b/modules/local/bagel2/bf.nf index e2db4e55..f4e8b5f5 100644 --- a/modules/local/bagel2/bf.nf +++ b/modules/local/bagel2/bf.nf @@ -1,5 +1,5 @@ process BAGEL2_BF { - tag "$meta.treatment" + tag "${meta.treatment}_${meta.reference}" label 'process_single' diff --git a/modules/local/bagel2/graph.nf b/modules/local/bagel2/graph.nf index d7aad230..c95dc3fc 100644 --- a/modules/local/bagel2/graph.nf +++ b/modules/local/bagel2/graph.nf @@ -19,7 +19,7 @@ process BAGEL2_GRAPH { script: def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" + def prefix = task.ext.prefix ?: "${meta.treatment}_${meta.reference}" """ #!/usr/bin/env python3 diff --git a/modules/local/bagel2/pr.nf b/modules/local/bagel2/pr.nf index 2b8b8d5a..7b3c4955 100644 --- a/modules/local/bagel2/pr.nf +++ b/modules/local/bagel2/pr.nf @@ -20,7 +20,7 @@ process BAGEL2_PR { script: def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" + def prefix = task.ext.prefix ?: "${meta.treatment}_vs_${meta.reference}" """ BAGEL.py pr -i $bf -o '${meta.treatment}_vs_${meta.reference}.tsv' -e $reference_essentials -n $reference_nonessentials $args diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index c65dea38..fe68748c 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -11,6 +11,7 @@ include { BAGEL2_PR } from '../modules/local/ include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' include { MATRICESCREATION } from '../modules/local/matricescreation' include { MAGECK_FLUTEMLE } from '../modules/local/mageck/flutemle' +include { VENNDIAGRAM } from '../modules/local/venndiagram' // nf-core modules include { FASTQC } from '../modules/nf-core/fastqc/main' include { CUTADAPT as CUTADAPT_THREE_PRIME } from '../modules/nf-core/cutadapt/main' @@ -223,6 +224,7 @@ workflow CRISPRSEQ_SCREENING { .set { ch_contrasts } counts = ch_contrasts.combine(ch_counts) + //Define non essential and essential genes channels for bagel2 ch_bagel_reference_essentials= Channel.fromPath(params.bagel_reference_essentials).first() ch_bagel_reference_nonessentials= Channel.fromPath(params.bagel_reference_nonessentials).first() @@ -247,6 +249,7 @@ workflow CRISPRSEQ_SCREENING { BAGEL2_PR ( ch_bagel_pr ) + BAGEL2_PR.out.pr.dump(tag: "COUNTS") ch_versions = ch_versions.mix(BAGEL2_PR.out.versions) @@ -260,36 +263,6 @@ workflow CRISPRSEQ_SCREENING { if((params.mle_design_matrix) || (params.contrasts && !params.rra) || (params.day0_label)) { if(params.mle_design_matrix) { -<<<<<<< HEAD - ch_mle = ch_counts.combine(ch_design) - } - if(params.contrasts) { - MATRICESCREATION(Channel.fromPath(params.contrasts)) - ch_mle = ch_counts.combine(MATRICESCREATION.out.design_matrix) - } - ch_mle.map { - it -> [[id: it[1].getBaseName()], it[0], it[1]] - }.set { ch_designed_mle } - ch_designed_mle.dump(tag: "ch_designed_mle") - //TODO TIDY UP THIS CODE - BAGEL2_PR.out.pr.map { - it -> [[id: it[1].getBaseName()], it[1]] - }.set { ch_testing } - //ch_testing.dump(tag: "BAGEL2 out PR") - - MAGECK_MLE ( - ch_designed_mle - ) - ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) - - ch_venndiagram = ch_testing.join(MAGECK_MLE.out.gene_summary) - MAGECK_MLE.out.gene_summary.dump(tag: "MLE") - ch_venndiagram.dump(tag: "Venn") - VENNDIAGRAM(ch_venndiagram) - - - -======= INITIALISATION_CHANNEL_CREATION_SCREENING.out.design.map { it -> [[id: it.getBaseName()], it] }.set { ch_designed_mle } @@ -307,6 +280,17 @@ workflow CRISPRSEQ_SCREENING { ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) MAGECK_FLUTEMLE(MAGECK_MLE.out.gene_summary) ch_versions = ch_versions.mix(MAGECK_FLUTEMLE.out.versions) + + //test Venn Diagram + BAGEL2_PR.out.pr.map { + it -> [[id: it[1].getBaseName()], it[1]] + }.set { ch_testing } + ch_testing.dump(tag: "TEST") + ch_venndiagram = BAGEL2_PR.out.pr.join(MAGECK_MLE.out.gene_summary) + MAGECK_MLE.out.gene_summary.dump(tag: "MAGECK MLE OUT") + ch_venndiagram.dump(tag: "Venn") + VENNDIAGRAM(ch_venndiagram) + } if(params.day0_label) { ch_mle = Channel.of([id: "day0"]).merge(Channel.of([[]])).merge(ch_counts) @@ -315,7 +299,6 @@ workflow CRISPRSEQ_SCREENING { MAGECK_FLUTEMLE(MAGECK_MLE_DAY0.out.gene_summary) ch_versions = ch_versions.mix(MAGECK_FLUTEMLE.out.versions) } ->>>>>>> nf-core/dev } // From 3c6bac755c8134863f5423565082d50b293cc20f Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 27 Mar 2024 10:45:02 +0100 Subject: [PATCH 101/226] Ran pre commit --- modules/local/venndiagram.nf | 30 +++++++++++++++--------------- 1 file changed, 15 insertions(+), 15 deletions(-) diff --git a/modules/local/venndiagram.nf b/modules/local/venndiagram.nf index 25f00edc..c75cc037 100644 --- a/modules/local/venndiagram.nf +++ b/modules/local/venndiagram.nf @@ -1,5 +1,5 @@ process VENNDIAGRAM { - // tag "$meta.treatment" + // tag "$meta.treatment" label 'process_low' @@ -50,25 +50,25 @@ process VENNDIAGRAM { filename = '14_venn_diagramm.png', output=FALSE ) - # venn.plot <- venn.diagram(list(Set1 = filtered_mageck_mle\$Gene, Set2 = filtered_precision_recall\$Gene), + # venn.plot <- venn.diagram(list(Set1 = filtered_mageck_mle\$Gene, Set2 = filtered_precision_recall\$Gene), # filename= paste0('${meta.id}',"_venn.svg"), category.names = c("MAGeCK MLE", "BAGEL2"), - # # Output features - # imagetype = "svg", - # height = 480, + # # Output features + # imagetype = "svg", + # height = 480, # width = 480, - # resolution = 300, - # compression = "lzw", + # resolution = 300, + # compression = "lzw", - # # Circles - # lwd = 2, - # lty = 'blank', - # fill = c("#999999", "#E69F00"), + # # Circles + # lwd = 2, + # lty = 'blank', + # fill = c("#999999", "#E69F00"), - # # Numbers + # # Numbers # cex = .6, - # fontface = "bold", - # fontfamily = "sans", - # cat.cex = 0.4, + # fontface = "bold", + # fontfamily = "sans", + # cat.cex = 0.4, # cat.dist = c(-0.05, -0.02) #) From 4e49371be748964e80a023daa75485e959b5e05e Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 27 Mar 2024 13:05:58 +0100 Subject: [PATCH 102/226] Add docs and functionning code --- conf/modules.config | 8 ++++++ docs/output/screening.md | 11 ++++++++- docs/usage/screening.md | 4 +++ modules/local/venndiagram.nf | 48 ++++++++---------------------------- 4 files changed, 32 insertions(+), 39 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 59b9c7ca..525408d8 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -135,6 +135,14 @@ process { ] } + withName: VENNDIAGRAM { + publishDir = [ + path: { "${params.outdir}/venndiagram/${meta.treatment}_vs_${meta.reference}/" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + withName: MAGECK_MLE_DAY0 { ext.args = "--day0-label '${params.day0_label}'" publishDir = [ diff --git a/docs/output/screening.md b/docs/output/screening.md index e964991b..8c1cd6e5 100644 --- a/docs/output/screening.md +++ b/docs/output/screening.md @@ -148,7 +148,16 @@ For further reading and documentation see the [cutadapt helper page](https://cut [bagel2](https://github.com/hart-lab/bagel) is a computational tool to identify important essential genes for CRISPR-Cas9 screening experiments. -## Gene essentiality computation +### Venn diagram + +
    +Output files + +- `venndiagram` + - `*_common_genes_bagel_mle.txt`: common essential genes between BAGEL2 and MAGeCK MLE + - `*_venn_bagel2_mageckmle.png`: Venn diagram common essential genes between BAGEL2 and MAGeCK MLE + +## Gene essentiality functional analysis ### MAGeCKFlute diff --git a/docs/usage/screening.md b/docs/usage/screening.md index 922cb671..3213993e 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -83,6 +83,10 @@ To run both MAGeCK MLE and BAGEL2, you can provide a contrast file with the flag A full example can be found [here](https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/full_test/samplesheet_full.csv). +#### Venn diagram + +Running MAGeCK MLE and BAGEL2 with a contrast file will also output a Venn diagram showing common genes having an FDR < 0.1 + ### Running MAGeCK RRA only MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section (with a `.txt` extension) and also specify `--rra` . diff --git a/modules/local/venndiagram.nf b/modules/local/venndiagram.nf index c75cc037..7da3332d 100644 --- a/modules/local/venndiagram.nf +++ b/modules/local/venndiagram.nf @@ -1,12 +1,10 @@ process VENNDIAGRAM { - // tag "$meta.treatment" + tag "${meta.treatment}_vs_${meta.reference}" label 'process_low' conda "bioconda::r-venndiagram=1.6.16" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/r-venndiagram:1.6.16--r3.3.2_0': - 'biocontainers/r-venndiagram:1.6.16--r3.3.2_0' }" + container "ghcr.io/qbic-pipelines/rnadeseq:dev" input: tuple val(meta), path(bagel_pr), path(gene_summary) @@ -20,7 +18,7 @@ process VENNDIAGRAM { script: def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.treatment}" + def prefix = task.ext.prefix ?: "${meta.treatment}_vs_${meta.reference}" """ #!/usr/bin/env Rscript @@ -29,49 +27,23 @@ process VENNDIAGRAM { #### Released under the MIT license. See git repository (https://github.com/nf-core/crisprseq) for full license text. #### #### produce a venn diagram - - library(VennDiagram) + library(ggvenn) mle = read.table('$gene_summary', sep = "\t", header=TRUE) bagel = read.table('$bagel_pr', sep = "\t", header=TRUE) filtered_precision_recall <- subset(bagel, FDR > 0.1) - name <- paste0('${meta.id}',".fdr") + name <- paste0('${prefix}',".fdr") filtered_mageck_mle <- subset(mle,name > 0.1) common_genes <- intersect(filtered_mageck_mle\$Gene, filtered_precision_recall\$Gene) + data <- list(Bagel2 = filtered_precision_recall\$Gene, + MAGeCK_MLE = filtered_mageck_mle\$Gene) - write.table(common_genes, paste0('${meta.id}',"_common_genes_bagel_mle.txt"),sep = "\t", quote = FALSE, row.names=FALSE) - - venn.diagram( - x = list(Set1 = filtered_mageck_mle\$Gene, Set2 = filtered_precision_recall\$Gene), - category.names = c("MAGeCK MLE", "BAGEL2"), - filename = '14_venn_diagramm.png', - output=FALSE - ) - # venn.plot <- venn.diagram(list(Set1 = filtered_mageck_mle\$Gene, Set2 = filtered_precision_recall\$Gene), - # filename= paste0('${meta.id}',"_venn.svg"), category.names = c("MAGeCK MLE", "BAGEL2"), - # # Output features - # imagetype = "svg", - # height = 480, - # width = 480, - # resolution = 300, - # compression = "lzw", - - # # Circles - # lwd = 2, - # lty = 'blank', - # fill = c("#999999", "#E69F00"), - - # # Numbers - # cex = .6, - # fontface = "bold", - # fontfamily = "sans", - # cat.cex = 0.4, - # cat.dist = c(-0.05, -0.02) - #) - + plot_test <- ggvenn(data) + ggsave("venn_bagel2_mageckmle.png",plot_test) + write.table(common_genes, paste0('${prefix}',"_common_genes_bagel_mle.txt"),sep = "\t", quote = FALSE, row.names=FALSE) """ From afbd021c9e4e5909128d99bc29a7bdd7a4354d14 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 27 Mar 2024 13:25:26 +0100 Subject: [PATCH 103/226] Add versions --- modules/local/venndiagram.nf | 10 ++++++++++ workflows/crisprseq_screening.nf | 12 +----------- 2 files changed, 11 insertions(+), 11 deletions(-) diff --git a/modules/local/venndiagram.nf b/modules/local/venndiagram.nf index 7da3332d..b97c2f2c 100644 --- a/modules/local/venndiagram.nf +++ b/modules/local/venndiagram.nf @@ -12,6 +12,7 @@ process VENNDIAGRAM { output: tuple val(meta), path("*.txt"), emit: common_list tuple val(meta), path("*.png"), emit: venn_diagram + path "versions.yml", emit: versions when: task.ext.when == null || task.ext.when @@ -45,6 +46,15 @@ process VENNDIAGRAM { ggsave("venn_bagel2_mageckmle.png",plot_test) write.table(common_genes, paste0('${prefix}',"_common_genes_bagel_mle.txt"),sep = "\t", quote = FALSE, row.names=FALSE) + #version + version_file_path <- "versions.yml" + version_ggvenn <- paste(unlist(packageVersion("ggvenn")), collapse = ".") + f <- file(version_file_path, "w") + writeLines('"${task.process}":', f) + writeLines(" ggvenn: ", f, sep = "") + writeLines(version_ggvenn, f) + close(f) + """ diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index fe68748c..cd4716da 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -249,8 +249,6 @@ workflow CRISPRSEQ_SCREENING { BAGEL2_PR ( ch_bagel_pr ) - BAGEL2_PR.out.pr.dump(tag: "COUNTS") - ch_versions = ch_versions.mix(BAGEL2_PR.out.versions) BAGEL2_GRAPH ( @@ -280,17 +278,9 @@ workflow CRISPRSEQ_SCREENING { ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) MAGECK_FLUTEMLE(MAGECK_MLE.out.gene_summary) ch_versions = ch_versions.mix(MAGECK_FLUTEMLE.out.versions) - - //test Venn Diagram - BAGEL2_PR.out.pr.map { - it -> [[id: it[1].getBaseName()], it[1]] - }.set { ch_testing } - ch_testing.dump(tag: "TEST") ch_venndiagram = BAGEL2_PR.out.pr.join(MAGECK_MLE.out.gene_summary) - MAGECK_MLE.out.gene_summary.dump(tag: "MAGECK MLE OUT") - ch_venndiagram.dump(tag: "Venn") VENNDIAGRAM(ch_venndiagram) - + ch_versions = ch_versions.mix(VENNDIAGRAM.out.versions) } if(params.day0_label) { ch_mle = Channel.of([id: "day0"]).merge(Channel.of([[]])).merge(ch_counts) From ead92ec8f9d16834e5a6667eaa6779c5b37debf9 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Wed, 27 Mar 2024 13:55:28 +0100 Subject: [PATCH 104/226] Update modules/local/matricescreation.nf MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- modules/local/matricescreation.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/modules/local/matricescreation.nf b/modules/local/matricescreation.nf index 9a6d8b56..001d8f1a 100644 --- a/modules/local/matricescreation.nf +++ b/modules/local/matricescreation.nf @@ -22,7 +22,6 @@ process MATRICESCREATION { #### author: Laurence Kuhlburger #### Released under the MIT license. See git repository (https://github.com/nf-core/crisprseq) for full license text. #### - #### # Loop through each row in the data control_samples <- unlist(strsplit('${meta.reference}', ",")) From 3e35be62983588f8345d4f5007251be47c9290c7 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Wed, 27 Mar 2024 14:04:03 +0100 Subject: [PATCH 105/226] Update docs/usage/screening.md MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- docs/usage/screening.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage/screening.md b/docs/usage/screening.md index 3213993e..d9939bf4 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -85,7 +85,7 @@ A full example can be found [here](https://raw.githubusercontent.com/nf-core/tes #### Venn diagram -Running MAGeCK MLE and BAGEL2 with a contrast file will also output a Venn diagram showing common genes having an FDR < 0.1 +Running MAGeCK MLE 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An example is shown here below + +![Venn diagram](docs/images/venn.png) ## Gene essentiality functional analysis From d290ce2ae661290d5a24149d6d3d5d627b9c34db Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 11 Apr 2024 15:51:34 +0200 Subject: [PATCH 107/226] Configure multiqc for mageck --- assets/multiqc_config.yml | 36 ++++++++++++++++++++++++++++++++ workflows/crisprseq_screening.nf | 1 + 2 files changed, 37 insertions(+) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 1e3b44b6..55527360 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -143,6 +143,39 @@ custom_data: min: 0 scale: "RdYlGn" + # MAGeCK count table + mageck_count: + id: "mageck_count" + section_name: "MAGeCK count table" + plot_type: "table" + description: | + Table showing ... + pconfig: + id: "mageck_count" + namespace: "MAGeCK count table" + table_title: "MAGeCK count table" + headers: + File: + title: "File" + description: "File name" + Label: + title: "Label" + description: "Label name" + format: "{:,.0f}" + Reads: + title: "Reads" + description: "Number of reads" + format: "{:,.0f}" + Mapped: + title: "Mapped" + description: "Number of reads mapped" + format: "{:,.0f}" + Percentage: + title: "Percentage mapped" + description: "Percentage mapped" + max: 1 + min: 0 + sp: edition_plot: fn: "*_edits.csv" @@ -152,12 +185,15 @@ sp: fn: "*_reads-summary.csv" cutadapt: fn: "*.cutadapt.log" + mageck_count: + fn: "*.countsummary.txt" # Define the order of sections module_order: - fastqc - cutadapt - custom_content + - mageck_count # Set the order of custom code plots and tables custom_content: diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index a2905b90..23bdc7d9 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -355,6 +355,7 @@ workflow CRISPRSEQ_SCREENING { ch_multiqc_files = Channel.empty() ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(MAGECK_COUNT.out.summary.collect{it[1]}.ifEmpty([])) ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) if(!params.count_table) { ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) From 421c1a772afac8396f54e85c0920f11603754f71 Mon Sep 17 00:00:00 2001 From: mashehu Date: Fri, 12 Apr 2024 10:54:46 +0200 Subject: [PATCH 108/226] fix link to samplesheet --- docs/usage/targeted.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/docs/usage/targeted.md b/docs/usage/targeted.md index 4c20f20f..93a28756 100644 --- a/docs/usage/targeted.md +++ b/docs/usage/targeted.md @@ -35,7 +35,7 @@ CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz,G The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 6 columns to match those defined in the table below. -A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 3 samples, where `chr6` is single-end and has a template sequence _(this is a reduced samplesheet, please refer to the [pipeline example saplesheet](https://nf-co.re/crisprseq/1.0/assets/samplesheet.csv) to see the full version)_. +A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 3 samples, where `chr6` is single-end and has a template sequence _(this is a reduced samplesheet, please refer to the [pipeline example saplesheet](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/samplesheet_test.csv) to see the full version)_. ```console sample,fastq_1,fastq_2,reference,protospacer,template @@ -53,7 +53,7 @@ chr6,chr6-61942198-61942498_R1.fastq.gz,,CAA...GGA,TTTTATGATATTTATCTTTT,TTC...CA | `protospacer` | Sequence of the protospacer used for CRISPR editing. Must not includ the PAM. | | `template` | Sequence of the template used in templet-based editing experiments. 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index 73aa4d58..483e98ed 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -13,6 +13,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Added bowtie2 and three prime and five prime adapter trimming ([#103](https://github.com/nf-core/crisprseq/pull/103) and [#123](https://github.com/nf-core/crisprseq/pull/123)) - Added `--day0_label` and `FluteMLE` for MLE data [#126](https://github.com/nf-core/crisprseq/pull/126) - Template update to 2.13.1 ([#124](https://github.com/nf-core/crisprseq/pull/124)) +- Metromap added in the docs ### Fixed diff --git a/README.md b/README.md index 315ec773..d4e46566 100644 --- a/README.md +++ b/README.md @@ -34,11 +34,9 @@ On release, automated continuous integration tests run the pipeline on a full-si ## Pipeline summary -For crispr targeted: - - - Text changing depending on mode. Light: 'So light!' Dark: 'So dark!' + + Text changing depending on mode. Light: 'So light!' Dark: 'So dark!' 1. Merge paired-end reads ([`Pear`](https://cme.h-its.org/exelixis/web/software/pear/doc.html)) From 4cdcd6ba66545f6a713ec1b77f4a0b74eb1cdf68 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Matthias=20H=C3=B6rtenhuber?= Date: Fri, 12 Apr 2024 11:05:58 +0200 Subject: [PATCH 111/226] Update docs/usage/targeted.md Co-authored-by: LaurenceKuhl --- docs/usage/targeted.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage/targeted.md b/docs/usage/targeted.md index 93a28756..d1ddad37 100644 --- a/docs/usage/targeted.md +++ b/docs/usage/targeted.md @@ -35,7 +35,7 @@ CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz,G The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 6 columns to match those defined in the table below. -A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 3 samples, where `chr6` is single-end and has a template sequence _(this is a reduced samplesheet, please refer to the [pipeline example saplesheet](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/samplesheet_test.csv) to see the full version)_. +A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 3 samples, where `chr6` is single-end and has a template sequence _(this is a reduced samplesheet, please refer to the [pipeline example samplesheet](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/samplesheet_test.csv) to see the full version)_. ```console sample,fastq_1,fastq_2,reference,protospacer,template From bf52a4bd0f9b04be1a0ff9ce31cec88df74c5676 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 12 Apr 2024 11:11:23 +0200 Subject: [PATCH 112/226] With the PR number --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 483e98ed..3fcd12ff 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -13,7 +13,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Added bowtie2 and three prime and five prime adapter trimming ([#103](https://github.com/nf-core/crisprseq/pull/103) and [#123](https://github.com/nf-core/crisprseq/pull/123)) - Added `--day0_label` and `FluteMLE` for MLE data [#126](https://github.com/nf-core/crisprseq/pull/126) - Template update to 2.13.1 ([#124](https://github.com/nf-core/crisprseq/pull/124)) -- Metromap added in the docs +- Metromap added in the docs ([#128](https://github.com/nf-core/crisprseq/pull/128)) ### Fixed From 532a724419da3c5c7cad9b3482ad65c049cb521e Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Mon, 15 Apr 2024 16:41:29 +0200 Subject: [PATCH 113/226] modifications suggested --- docs/images/crisprseq_metromap_all.png | Bin 301683 -> 295213 bytes 1 file changed, 0 insertions(+), 0 deletions(-) diff --git a/docs/images/crisprseq_metromap_all.png b/docs/images/crisprseq_metromap_all.png index 458d7c8c86c1294547f0cf2e2bf6b7c1aa16c03f..35fbea25e170a3400932c5427dcf066ef44fceba 100644 GIT binary patch literal 295213 zcmZs@cOaH+{5F1 z5?R0F>UqEK?~nKUM?Fv6_jO;_d49(6IgaDJA1W!H-?o)yD~Uwfc0pd|Dv3nRN+MC{ zZP|>Uj9(9s!GEZ&rDBu>%=nX@X6(Gy?ps56W%l+R92-1DH^K@(?k=^iCbhsh}xW%Dk* zgo=m{!5<7# 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b/README.md @@ -39,6 +39,9 @@ On release, automated continuous integration tests run the pipeline on a full-si Text changing depending on mode. Light: 'So light!' Dark: 'So dark!' + +For crispr targeting : + 1. Merge paired-end reads ([`Pear`](https://cme.h-its.org/exelixis/web/software/pear/doc.html)) 2. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 3. Adapter trimming ([`Cutadapt`](http://dx.doi.org/10.14806/ej.17.1.200)) @@ -65,6 +68,8 @@ For crispr screening: 4. Rank sgRNAs and genes ; a. ([MAGeCK test](https://sourceforge.net/p/mageck/wiki/usage/#test)) b. ([MAGeCK mle](https://sourceforge.net/p/mageck/wiki/Home/#mle)) + c. ([BAGEL2](https://github.com/hart-lab/bagel)) +5. 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Light: 'So light!' Dark: 'So dark!' - For crispr targeting : 1. 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zbm7u=h99=4tn@Fh#eG~5S2cig?iAk2YGSKy$Dr_)X;ss^v|B`XPo~%E{fyl+?ZRbe zw}x*#GfF z?U~!=|NY>w^nYIFzYo9O|Icas=RsKd<$vz=e}5o;-zUBQe*gDZ@ACuO|M!D)rvLY{ m1pfab{@I|$|5tY+;nya0`r2?*$Kpx;n Date: Thu, 18 Apr 2024 15:12:11 +0200 Subject: [PATCH 120/226] Matricescreation fixed --- modules/local/matricescreation.nf | 8 +++++--- 1 file changed, 5 insertions(+), 3 deletions(-) diff --git a/modules/local/matricescreation.nf b/modules/local/matricescreation.nf index 001d8f1a..86c00ee4 100644 --- a/modules/local/matricescreation.nf +++ b/modules/local/matricescreation.nf @@ -16,6 +16,7 @@ process MATRICESCREATION { task.ext.when == null || task.ext.when script: + meta.id = "${meta.treatment}_vs_${meta.reference}" """ #!/usr/bin/env Rscript @@ -24,14 +25,15 @@ process MATRICESCREATION { #### # Loop through each row in the data - control_samples <- unlist(strsplit('${meta.reference}', ",")) + control_samples <- unlist(strsplit('$meta.reference', ",")) treatment_samples <- unlist(strsplit('$meta.treatment', ",")) all_samples <- unique(c(control_samples, treatment_samples)) + name = paste0(gsub(',', '_', '$meta.treatment' ),"_vs_", gsub(',', '_','$meta.reference')) design_matrix <- data.frame(matrix(0, nrow = length(all_samples), ncol = 3, dimnames = list(all_samples, c("Samples", "baseline", - paste0(gsub(',', '_', '$meta.treatment'),"_vs_",gsub(',','_','$meta.reference')))))) - name = paste0(gsub(',', '_', '$meta.treatment' ),"_vs_", gsub(',', '_','$meta.reference')) + name)))) + # Set baseline and treatment values in the design matrix design_matrix[, "Samples"] <- rownames(design_matrix) design_matrix\$baseline <- 1 From 1a930abde26e1f6beb8978b55ba23fc3517519f8 Mon Sep 17 00:00:00 2001 From: Mike Schaffer <134545161+mschaffer-incyte@users.noreply.github.com> Date: Mon, 22 Apr 2024 12:15:26 -0400 Subject: [PATCH 121/226] Update tower.yml with additional reports. --- tower.yml | 31 +++++++++++++++++++++++++++++++ 1 file changed, 31 insertions(+) diff --git a/tower.yml b/tower.yml index 787aedfe..91e8d40e 100644 --- a/tower.yml +++ b/tower.yml @@ -3,3 +3,34 @@ reports: display: "MultiQC HTML report" samplesheet.csv: display: "Auto-created samplesheet with collated metadata and FASTQ paths" + "plots/*accumulative.html": + display: "Accumulative deletions and insertions" + "plots/*delAlleles_plot.png": + display: "Image showing the most common deletions found." + "plots/*Deletions.html": + display: "Interactive barplot showing the percentage of reads showing a deletion for each position and the deletion sizes" + "plots/*Insertions.html": + display: "Interactive barplot showing the percentage of reads showing an insertion for each position as well as the insertion sizes" + "plots/*subs-perc_plot_LOGO.png": + display: "LOGO showing the most represented nucleotide and its percentage (y-axis) for protospacer positions" + "plots/*subs-perc_plot.png": + display: "Barplot showing the most represented nucleotide and its percentage (y-axis and bar tags) for +/-25 positions surrounding the cut site" + "plots/*top-alleles_LOGO.png": + display: "LOGO showing the 4 most represented editions" + "plots/*top.html": + display: "Interactive pie chart showing the percentage of the top 4 editions found" + "cigar/*_cutSite.json": + display: "Contains the protospacer cut site position in the reference." + "cigar/*_edition.html": + display: "Interactive pie chart with the percentage of edition types" + "cigar/*_edits.csv": + display: "Table containing the number of reads classified to each edition type" + "cigar/*_indels.csv": + display: "Table containing information of all reads" + "cigar/*_QC-indels.html": + display: "Interactive pie chart with information about aligned reads" + "cigar/*_reads.html": + display: "Interactive pie chart with percentage of the number of raw reads, reads merged with Pear, reads passing quality filters and UMI clustered reads" + "cigar/*_subs-perc.csv": + display: "Table containing the percentage of each nucleotide found for each reference position" + From b3a5b2f6617e4879422eb10994da0969f5e94b09 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 24 Apr 2024 11:21:22 +0200 Subject: [PATCH 122/226] Fix error on nextflow edge version --- conf/modules.config | 1 + workflows/crisprseq_targeted.nf | 10 ---------- 2 files changed, 1 insertion(+), 10 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 525408d8..044eec00 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -322,6 +322,7 @@ process { withName: MINIMAP2_ALIGN_TEMPLATE { ext.args = '-A 29 -B 17 -O 25 -E 2' + ext.prefix = {"${meta.id}_template-align"} publishDir = [ path: { "${params.outdir}/preprocessing/sequences" }, mode: params.publish_dir_mode, diff --git a/workflows/crisprseq_targeted.nf b/workflows/crisprseq_targeted.nf index add1f805..3e0a02dc 100644 --- a/workflows/crisprseq_targeted.nf +++ b/workflows/crisprseq_targeted.nf @@ -578,16 +578,6 @@ workflow CRISPRSEQ_TARGETED { true ) .bam - .map { - meta, bam -> - if (bam.baseName.contains("template-align")) { - return [ meta, bam ] - } else { - new_file = bam.parent / bam.baseName + "_template-align." + bam.extension - bam.renameTo(new_file) - return[ meta, new_file ] - } - } .set { ch_template_bam } ch_versions = ch_versions.mix(MINIMAP2_ALIGN_TEMPLATE.out.versions) From 1619309b23bda9bc2a5df289d2abd0194970c4c8 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 24 Apr 2024 15:07:40 +0200 Subject: [PATCH 123/226] Fix typos in the doc --- docs/output/screening.md | 2 +- docs/usage/screening.md | 11 ++++++----- 2 files changed, 7 insertions(+), 6 deletions(-) diff --git a/docs/output/screening.md b/docs/output/screening.md index 8685f60f..d4a7978e 100644 --- a/docs/output/screening.md +++ b/docs/output/screening.md @@ -157,7 +157,7 @@ For further reading and documentation see the [cutadapt helper page](https://cut - `*_common_genes_bagel_mle.txt`: common essential genes between BAGEL2 and MAGeCK MLE - `*_venn_bagel2_mageckmle.png`: Venn diagram common essential genes between BAGEL2 and MAGeCK MLE. An example is shown here below -![Venn diagram](docs/images/venn.png) +![Venn diagram](/docs/images/venn.png) ## Gene essentiality functional analysis diff --git a/docs/usage/screening.md b/docs/usage/screening.md index d9939bf4..339a73d6 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -64,13 +64,14 @@ We recommend to run MAGeCK MLE and BAGEL2 as these are the most used and most re CRISPRcleanR is used for gene count normalization and the removal of biases for genomic segments for which copy numbers are amplified. Currently, the pipeline supports annotation libraries already present in the R package or a annotation file the user can provide. Most used library already have an annotation dataset which you can find [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). -Otherwise, if you wish to provide your own file, please provide it in csv form, and make sure it follows the following format : +Otherwise, if you wish to provide your own file, please provide it in csv form, and make sure it follows the following format, with the comma in front of "CODE" included : | ,CODE | GENES | EXONE | CHRM | STRAND | STARTpos | ENDpos | -| -------------------- | ----------- | ------------- | ---- | ------ | -------- | -------- | -------- | -| ATGGTGTCCATTATAGCCAT | NM_021446.2 | 0610007P14Rik | ex2 | 12 | + | 85822165 | 85822185 | -| CTCTACGAGAAGCTCTACAC | NM_021446.2 | 0610007P14Rik | ex2 | 12 | + | 85822108 | 85822128 | -| GACTCTATCACATCACACTG | NM_021446.2 | 0610007P14Rik | ex4 | 12 | + | 85816419 | 85816439 | +| ------------------- | ----------- | ------------- | ---- | ------ | -------- | -------- | +| ATGGTGTCCATTATAGCCAT| NM_021446.2 | 0610007P14Rik | ex2 | 12 | + | 85822165 | +| CTCTACGAGAAGCTCTACAC| NM_021446.2 | 0610007P14Rik | ex2 | 12 | + | 85822108 | +| GACTCTATCACATCACACTG| NM_021446.2 | 0610007P14Rik | ex4 | 12 | + | 85816419 | + ### Running MAGeCK MLE and BAGEL2 with a contrast file From 1e52ca67a616ec2903060c4d8533e82b6ecb2a7b Mon Sep 17 00:00:00 2001 From: Mike Schaffer <134545161+mschaffer-incyte@users.noreply.github.com> Date: Wed, 24 Apr 2024 13:45:33 -0400 Subject: [PATCH 124/226] Update tower.yml with shortened report names --- tower.yml | 45 ++++++++++++++++++++++++++++++--------------- 1 file changed, 30 insertions(+), 15 deletions(-) diff --git a/tower.yml b/tower.yml index 91e8d40e..e45066bf 100644 --- a/tower.yml +++ b/tower.yml @@ -4,33 +4,48 @@ reports: samplesheet.csv: display: "Auto-created samplesheet with collated metadata and FASTQ paths" "plots/*accumulative.html": - display: "Accumulative deletions and insertions" + # Accumulative deletions and insertions + display: "accumulative" "plots/*delAlleles_plot.png": - display: "Image showing the most common deletions found." + # Image showing the most common deletions found + display: "delAlleles_plot" "plots/*Deletions.html": - display: "Interactive barplot showing the percentage of reads showing a deletion for each position and the deletion sizes" + # Interactive barplot showing the percentage of reads showing a deletion for each position and the deletion sizes + display: "Deletions" "plots/*Insertions.html": - display: "Interactive barplot showing the percentage of reads showing an insertion for each position as well as the insertion sizes" + # Interactive barplot showing the percentage of reads showing an insertion for each position as well as the insertion sizes + display: "Insertions" "plots/*subs-perc_plot_LOGO.png": - display: "LOGO showing the most represented nucleotide and its percentage (y-axis) for protospacer positions" + # LOGO showing the most represented nucleotide and its percentage (y-axis) for protospacer positions + display: "subs-perc_plot_LOGO" "plots/*subs-perc_plot.png": - display: "Barplot showing the most represented nucleotide and its percentage (y-axis and bar tags) for +/-25 positions surrounding the cut site" + # Barplot showing the most represented nucleotide and its percentage (y-axis and bar tags) for +/-25 positions surrounding the cut site + display: "subs-perc_plot" "plots/*top-alleles_LOGO.png": - display: "LOGO showing the 4 most represented editions" + # LOGO showing the 4 most represented editions + display: "top-alleles_LOGO" "plots/*top.html": - display: "Interactive pie chart showing the percentage of the top 4 editions found" + # Interactive pie chart showing the percentage of the top 4 editions found + display: "top" "cigar/*_cutSite.json": - display: "Contains the protospacer cut site position in the reference." + # Contains the protospacer cut site position in the reference + display: "cutSite" "cigar/*_edition.html": - display: "Interactive pie chart with the percentage of edition types" + # Interactive pie chart with the percentage of edition types + display: "edition" "cigar/*_edits.csv": - display: "Table containing the number of reads classified to each edition type" + # Table containing the number of reads classified to each edition type + display: "edits" "cigar/*_indels.csv": - display: "Table containing information of all reads" + # Table containing information of all reads + display: "indels" "cigar/*_QC-indels.html": - display: "Interactive pie chart with information about aligned reads" + # Interactive pie chart with information about aligned reads + display: "QC-indels" "cigar/*_reads.html": - display: "Interactive pie chart with percentage of the number of raw reads, reads merged with Pear, reads passing quality filters and UMI clustered reads" + # Interactive pie chart with percentage of the number of raw reads, reads merged with Pear, reads passing quality filters and UMI clustered reads + display: "reads" "cigar/*_subs-perc.csv": - display: "Table containing the percentage of each nucleotide found for each reference position" + # Table containing the percentage of each nucleotide found for each reference position + display: "subs-perc" From b16bca7accc997136249151d136f0e8a15804e71 Mon Sep 17 00:00:00 2001 From: Mike Schaffer <134545161+mschaffer-incyte@users.noreply.github.com> Date: Wed, 24 Apr 2024 16:54:48 -0400 Subject: [PATCH 125/226] Update tower.yml --- tower.yml | 15 --------------- 1 file changed, 15 deletions(-) diff --git a/tower.yml b/tower.yml index e45066bf..a8d4cf52 100644 --- a/tower.yml +++ b/tower.yml @@ -4,48 +4,33 @@ reports: samplesheet.csv: display: "Auto-created samplesheet with collated metadata and FASTQ paths" "plots/*accumulative.html": - # Accumulative deletions and insertions display: "accumulative" "plots/*delAlleles_plot.png": - # Image showing the most common deletions found display: "delAlleles_plot" "plots/*Deletions.html": - # Interactive barplot showing the percentage of reads showing a deletion for each position and the deletion sizes display: "Deletions" "plots/*Insertions.html": - # Interactive barplot showing the percentage of reads showing an insertion for each position as well as the insertion sizes display: "Insertions" "plots/*subs-perc_plot_LOGO.png": - # LOGO showing the most represented nucleotide and its percentage (y-axis) for protospacer positions display: "subs-perc_plot_LOGO" "plots/*subs-perc_plot.png": - # Barplot showing the most represented nucleotide and its percentage (y-axis and bar tags) for +/-25 positions surrounding the cut site display: "subs-perc_plot" "plots/*top-alleles_LOGO.png": - # LOGO showing the 4 most represented editions display: "top-alleles_LOGO" "plots/*top.html": - # Interactive pie chart showing the percentage of the top 4 editions found display: "top" "cigar/*_cutSite.json": - # Contains the protospacer cut site position in the reference display: "cutSite" "cigar/*_edition.html": - # Interactive pie chart with the percentage of edition types display: "edition" "cigar/*_edits.csv": - # Table containing the number of reads classified to each edition type display: "edits" "cigar/*_indels.csv": - # Table containing information of all reads display: "indels" "cigar/*_QC-indels.html": - # Interactive pie chart with information about aligned reads display: "QC-indels" "cigar/*_reads.html": - # Interactive pie chart with percentage of the number of raw reads, reads merged with Pear, reads passing quality filters and UMI clustered reads display: "reads" "cigar/*_subs-perc.csv": - # Table containing the percentage of each nucleotide found for each reference position display: "subs-perc" From 3e449ed52945fac23588a058576ca3e81ee5ec69 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 25 Apr 2024 13:11:32 +0200 Subject: [PATCH 126/226] Fix path metromap --- README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index a38d581e..3fc8da54 100644 --- a/README.md +++ b/README.md @@ -35,8 +35,8 @@ On release, automated continuous integration tests run the pipeline on a full-si ## Pipeline summary - - Text changing depending on mode. Light: 'So light!' Dark: 'So dark!' + + Text changing depending on mode. Light: 'So light!' Dark: 'So dark!' For crispr targeting : From b04d8d9348966084b5d7ba79ff3341e899c0c83a Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 25 Apr 2024 14:55:16 +0200 Subject: [PATCH 127/226] Ran pre commit --- docs/usage/screening.md | 9 ++++----- 1 file changed, 4 insertions(+), 5 deletions(-) diff --git a/docs/usage/screening.md b/docs/usage/screening.md index 339a73d6..4d375b64 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -67,11 +67,10 @@ Most used library already have an annotation dataset which you can find [here](h Otherwise, if you wish to provide your own file, please provide it in csv form, and make sure it follows the following format, with the comma in front of "CODE" included : | ,CODE | GENES | EXONE | CHRM | STRAND | STARTpos | ENDpos | -| ------------------- | ----------- | ------------- | ---- | ------ | -------- | -------- | -| ATGGTGTCCATTATAGCCAT| NM_021446.2 | 0610007P14Rik | ex2 | 12 | + | 85822165 | -| CTCTACGAGAAGCTCTACAC| NM_021446.2 | 0610007P14Rik | ex2 | 12 | + | 85822108 | -| GACTCTATCACATCACACTG| NM_021446.2 | 0610007P14Rik | ex4 | 12 | + | 85816419 | - +| -------------------- | ----------- | ------------- | ---- | ------ | -------- | -------- | +| ATGGTGTCCATTATAGCCAT | NM_021446.2 | 0610007P14Rik | ex2 | 12 | + | 85822165 | +| CTCTACGAGAAGCTCTACAC | NM_021446.2 | 0610007P14Rik | ex2 | 12 | + | 85822108 | +| GACTCTATCACATCACACTG | NM_021446.2 | 0610007P14Rik | ex4 | 12 | + | 85816419 | ### Running MAGeCK MLE and BAGEL2 with a contrast file From 2eb154bb5b55509cf7efd6e5324caa13fb255a99 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Sat, 27 Apr 2024 12:25:54 +0200 Subject: [PATCH 128/226] Add info in the readme --- README.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/README.md b/README.md index 3fc8da54..4d3c009d 100644 --- a/README.md +++ b/README.md @@ -63,6 +63,8 @@ For crispr screening: 1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 2. Read mapping ([`MAGeCK count`](https://sourceforge.net/p/mageck/wiki/usage/#count)) + - ([`MAGeCK count`](https://github.com/lh3/minimap2), _default_) + - ([`bwa`](http://bio-bwa.sourceforge.net/)) 3. Optional: CNV correction and normalization with ([`CRISPRcleanR`](https://github.com/francescojm/CRISPRcleanR)) 4. Rank sgRNAs and genes ; a. ([MAGeCK test](https://sourceforge.net/p/mageck/wiki/usage/#test)) From b5c8b46ba9b076e63ed022fb06092c94dc0761a4 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Sat, 27 Apr 2024 12:34:06 +0200 Subject: [PATCH 129/226] Add CO2 plugin --- nextflow.config | 1 + 1 file changed, 1 insertion(+) diff --git a/nextflow.config b/nextflow.config index a6c8003a..9d79ae9e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -219,6 +219,7 @@ singularity.registry = 'quay.io' // Nextflow plugins plugins { id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-co2footprint@1.0.0-beta' } // Load igenomes.config if required From 85e50c28d424a1df4e8cafc2979af00c3371c8bd Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Sat, 27 Apr 2024 13:29:53 +0200 Subject: [PATCH 130/226] Finish multiqc table --- README.md | 4 ++-- assets/multiqc_config.yml | 19 ++++++++++++++++--- workflows/crisprseq_screening.nf | 1 + 3 files changed, 19 insertions(+), 5 deletions(-) diff --git a/README.md b/README.md index 4d3c009d..cd3e0b78 100644 --- a/README.md +++ b/README.md @@ -64,13 +64,13 @@ For crispr screening: 1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 2. Read mapping ([`MAGeCK count`](https://sourceforge.net/p/mageck/wiki/usage/#count)) - ([`MAGeCK count`](https://github.com/lh3/minimap2), _default_) - - ([`bwa`](http://bio-bwa.sourceforge.net/)) + - ([`bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml)) 3. Optional: CNV correction and normalization with ([`CRISPRcleanR`](https://github.com/francescojm/CRISPRcleanR)) 4. Rank sgRNAs and genes ; a. ([MAGeCK test](https://sourceforge.net/p/mageck/wiki/usage/#test)) b. ([MAGeCK mle](https://sourceforge.net/p/mageck/wiki/Home/#mle)) c. ([BAGEL2](https://github.com/hart-lab/bagel)) -5. Visualise analysis +5. Visualize analysis ## Usage diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 3cb85df1..da1da3e0 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -150,7 +150,7 @@ custom_data: section_name: "MAGeCK count table" plot_type: "table" description: | - Table showing ... + Table showing count quality for MAGeCK count. pconfig: id: "mageck_count" namespace: "MAGeCK count table" @@ -172,10 +172,23 @@ custom_data: description: "Number of reads mapped" format: "{:,.0f}" Percentage: - title: "Percentage mapped" + title: "Percentage mapped from 0 to 1 (100%)" description: "Percentage mapped" max: 1 min: 0 + TotalsgRNAs: + title: "Total number of sgRNAs in the library" + description: "Total number of sgRNAs in the library" + format: "{:,.0f}" + Zerocounts: + title: "Total number of missing sgRNAs" + description: "sgRNAs that have 0 counts, recommended: no more than 1%)" + format: "{:,.0f}" + GiniIndex: + title: "Gini Index" + description: "Measure of statistical dispersion. A smaller value indicates more eveness of the count distribution." + max: 1 + min: 0 sp: edition_plot: @@ -193,8 +206,8 @@ sp: module_order: - fastqc - cutadapt - - custom_content - mageck_count + - custom_content # Set the order of custom code plots and tables custom_content: diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index cd4716da..8d5831da 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -161,6 +161,7 @@ workflow CRISPRSEQ_SCREENING { ) ch_versions = ch_versions.mix(MAGECK_COUNT.out.versions.first()) + ch_multiqc_files = ch_multiqc_files.mix(MAGECK_COUNT.out.summary.collect{it[1]}) MAGECK_COUNT.out.count.map { it -> it[1] From a5c30f4dd12b2c411178c5d25cfff26ef1c16cbc Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Sat, 27 Apr 2024 13:32:46 +0200 Subject: [PATCH 131/226] Update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 3fcd12ff..9450e48d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -14,6 +14,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Added `--day0_label` and `FluteMLE` for MLE data [#126](https://github.com/nf-core/crisprseq/pull/126) - Template update to 2.13.1 ([#124](https://github.com/nf-core/crisprseq/pull/124)) - Metromap added in the docs ([#128](https://github.com/nf-core/crisprseq/pull/128)) +- Added MAGeCK count table in the multiqc ([#131](https://github.com/nf-core/crisprseq/pull/131)) ### Fixed From a183b9312e751511dbd8760064784b5fd4624b5a Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Mon, 29 Apr 2024 16:13:51 +0200 Subject: [PATCH 132/226] Change version --- CHANGELOG.md | 2 +- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 3 files changed, 4 insertions(+), 4 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 3fcd12ff..74a5b5f9 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## [v2.2.0dev] +## [v3.3.0 - Rosmarin Curie] ### Added diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 804c9c98..3dc4ac4f 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/crisprseq + This report has been generated by the nf-core/crisprseq analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-crisprseq-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index 9d79ae9e..7490dae4 100644 --- a/nextflow.config +++ b/nextflow.config @@ -278,7 +278,7 @@ manifest { description = """Pipeline for the analysis of CRISPR data""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '2.2.0dev' + version = '3.0.0' doi = 'https://doi.org/10.5281/zenodo.7598496' } From bca0d330e5fa6d4b74c99c6aeb848f4a3ceecaed Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 2 May 2024 11:54:27 +0200 Subject: [PATCH 133/226] Fix crisprcleanr dir mode --- conf/modules.config | 1 + 1 file changed, 1 insertion(+) diff --git a/conf/modules.config b/conf/modules.config index 044eec00..d0b5594e 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -155,6 +155,7 @@ process { withName: CRISPRCLEANR_NORMALIZE { publishDir = [ path: { "${params.outdir}/crisprcleanr/normalization/" }, + mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } From c5f5ca5855b09b70e36be7b1e378da06a44a3c19 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 2 May 2024 14:56:15 +0200 Subject: [PATCH 134/226] Fix venn diagram --- modules/local/venndiagram.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/modules/local/venndiagram.nf b/modules/local/venndiagram.nf index b97c2f2c..868e6ad7 100644 --- a/modules/local/venndiagram.nf +++ b/modules/local/venndiagram.nf @@ -34,9 +34,9 @@ process VENNDIAGRAM { bagel = read.table('$bagel_pr', sep = "\t", header=TRUE) - filtered_precision_recall <- subset(bagel, FDR > 0.1) - name <- paste0('${prefix}',".fdr") - filtered_mageck_mle <- subset(mle,name > 0.1) + filtered_precision_recall <- subset(bagel, FDR < 0.1) + name <- gsub(",","_",paste0('${prefix}',".fdr")) + filtered_mageck_mle <- mle[mle[, name] < 0.1, ] common_genes <- intersect(filtered_mageck_mle\$Gene, filtered_precision_recall\$Gene) data <- list(Bagel2 = filtered_precision_recall\$Gene, From 60bd75e3fab40cf7a3e31b0391ccaa13a85b1d6b Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 2 May 2024 15:02:53 +0200 Subject: [PATCH 135/226] Force rollback in a very ugly way sorry --- CHANGELOG.md | 2 +- assets/multiqc_config.yml | 4 ++-- nextflow.config | 5 ++--- 3 files changed, 5 insertions(+), 6 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 74a5b5f9..3fcd12ff 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## [v3.3.0 - Rosmarin Curie] +## [v2.2.0dev] ### Added diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 3dc4ac4f..804c9c98 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/crisprseq + This report has been generated by the nf-core/crisprseq analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-crisprseq-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index 7490dae4..c86ccaa3 100644 --- a/nextflow.config +++ b/nextflow.config @@ -219,7 +219,6 @@ singularity.registry = 'quay.io' // Nextflow plugins plugins { id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet - id 'nf-co2footprint@1.0.0-beta' } // Load igenomes.config if required @@ -277,8 +276,8 @@ manifest { homePage = 'https://github.com/nf-core/crisprseq' description = """Pipeline for the analysis of CRISPR data""" mainScript = 'main.nf' - nextflowVersion = '!>=23.04.0' - version = '3.0.0' + nextflowVersion = '!>=23.07.0' + version = '2.2.0dev' doi = 'https://doi.org/10.5281/zenodo.7598496' } From e9ac0cda6ed6308d1811a36b4f1a5412526ca7b7 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 2 May 2024 15:15:34 +0200 Subject: [PATCH 136/226] Up nextflow version --- .../nf-core/utils_nextflow_pipeline/tests/nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index d0a926bf..9ce61c5b 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -3,7 +3,7 @@ manifest { author = """nf-core""" homePage = 'https://127.0.0.1' description = """Dummy pipeline""" - nextflowVersion = '!>=23.04.0' + nextflowVersion = '!>=23.07.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' } From 61f99930019b8e0b3c9dc5d00dfeb369b9d68e5a Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 2 May 2024 15:22:06 +0200 Subject: [PATCH 137/226] Change version in README --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index cd3e0b78..26be58f3 100644 --- a/README.md +++ b/README.md @@ -9,7 +9,7 @@ [![GitHub Actions Linting Status](https://github.com/nf-core/crisprseq/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/crisprseq/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/crisprseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.7598496-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.7598496) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.07.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) From 040fb4f82bd4ad69a2224be419e6128bd64049f4 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 2 May 2024 15:26:36 +0200 Subject: [PATCH 138/226] ughhh --- .../nf-core/utils_nfcore_pipeline/tests/nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config index d0a926bf..9ce61c5b 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -3,7 +3,7 @@ manifest { author = """nf-core""" homePage = 'https://127.0.0.1' description = """Dummy pipeline""" - nextflowVersion = '!>=23.04.0' + nextflowVersion = '!>=23.07.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' } From 1ed627677d8bd0782fb7f26dca4f0b210fdf971e Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 2 May 2024 15:34:41 +0200 Subject: [PATCH 139/226] add the ci.yml with the right NXF versioN --- .github/workflows/ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index e8695997..da71570a 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -24,7 +24,7 @@ jobs: strategy: matrix: NXF_VER: - - "23.04.0" + - "23.07.0" - "latest-everything" ANALYSIS: - "test_screening" From 0aaf5e8bc63aa4c12535e474491205482581c158 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Thu, 2 May 2024 16:31:47 +0200 Subject: [PATCH 140/226] Bump to version 24.07.0 --- .github/workflows/ci.yml | 2 +- README.md | 2 +- nextflow.config | 2 +- 3 files changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index da71570a..8e8c9c7f 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -24,7 +24,7 @@ jobs: strategy: matrix: NXF_VER: - - "23.07.0" + - "24.07.0" - "latest-everything" ANALYSIS: - "test_screening" diff --git a/README.md b/README.md index 26be58f3..a1aa3b58 100644 --- a/README.md +++ b/README.md @@ -9,7 +9,7 @@ [![GitHub Actions Linting Status](https://github.com/nf-core/crisprseq/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/crisprseq/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/crisprseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.7598496-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.7598496) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.07.0-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.07.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) diff --git a/nextflow.config b/nextflow.config index c86ccaa3..df93b9dd 100644 --- a/nextflow.config +++ b/nextflow.config @@ -276,7 +276,7 @@ manifest { homePage = 'https://github.com/nf-core/crisprseq' description = """Pipeline for the analysis of CRISPR data""" mainScript = 'main.nf' - nextflowVersion = '!>=23.07.0' + nextflowVersion = '!>=24.07.0' version = '2.2.0dev' doi = 'https://doi.org/10.5281/zenodo.7598496' } From 20fafccd06221ea326a0b25c0022db602c26d247 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 3 May 2024 10:46:38 +0200 Subject: [PATCH 141/226] Update bump version nextflow in subworkflows --- .../nf-core/utils_nextflow_pipeline/tests/nextflow.config | 2 +- .../nf-core/utils_nfcore_pipeline/tests/nextflow.config | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index 9ce61c5b..3178f1d3 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -3,7 +3,7 @@ manifest { author = """nf-core""" homePage = 'https://127.0.0.1' description = """Dummy pipeline""" - nextflowVersion = '!>=23.07.0' + nextflowVersion = '!>=24.07.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' } diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config index 9ce61c5b..3178f1d3 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -3,7 +3,7 @@ manifest { author = """nf-core""" homePage = 'https://127.0.0.1' description = """Dummy pipeline""" - nextflowVersion = '!>=23.07.0' + nextflowVersion = '!>=24.07.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' } From 05fc059baeb61a8e3defcd13cc07bfbdf2521d63 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 3 May 2024 11:13:13 +0200 Subject: [PATCH 142/226] update to 23.10.1 --- .github/workflows/ci.yml | 2 +- README.md | 2 +- .../nf-core/utils_nextflow_pipeline/tests/nextflow.config | 2 +- .../nf-core/utils_nfcore_pipeline/tests/nextflow.config | 2 +- 4 files changed, 4 insertions(+), 4 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 8e8c9c7f..1396108d 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -24,7 +24,7 @@ jobs: strategy: matrix: NXF_VER: - - "24.07.0" + - "23.10.1" - "latest-everything" ANALYSIS: - "test_screening" diff --git a/README.md b/README.md index a1aa3b58..1b0dceed 100644 --- a/README.md +++ b/README.md @@ -9,7 +9,7 @@ [![GitHub Actions Linting Status](https://github.com/nf-core/crisprseq/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/crisprseq/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/crisprseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.7598496-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.7598496) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.07.0-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.10.1-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index 3178f1d3..8f545add 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -3,7 +3,7 @@ manifest { author = """nf-core""" homePage = 'https://127.0.0.1' description = """Dummy pipeline""" - nextflowVersion = '!>=24.07.0' + nextflowVersion = '!>=23.10.1' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' } diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config index 3178f1d3..8f545add 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -3,7 +3,7 @@ manifest { author = """nf-core""" homePage = 'https://127.0.0.1' description = """Dummy pipeline""" - nextflowVersion = '!>=24.07.0' + nextflowVersion = '!>=23.10.1' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' } From 1f9a4572ea121579226691c8701bcafbb7961533 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 3 May 2024 11:17:52 +0200 Subject: [PATCH 143/226] Forgot the nextflow config --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index df93b9dd..b2fb9a86 100644 --- a/nextflow.config +++ b/nextflow.config @@ -276,7 +276,7 @@ manifest { homePage = 'https://github.com/nf-core/crisprseq' description = """Pipeline for the analysis of CRISPR data""" mainScript = 'main.nf' - nextflowVersion = '!>=24.07.0' + nextflowVersion = '!>=23.10.1' version = '2.2.0dev' doi = 'https://doi.org/10.5281/zenodo.7598496' } From 8baa19d150d9302fef847c17cf2cfe52a661dde1 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 3 May 2024 11:25:57 +0200 Subject: [PATCH 144/226] Let's just stick to the NXF version of the template for now --- .github/workflows/ci.yml | 2 +- README.md | 2 +- nextflow.config | 2 +- 3 files changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 1396108d..e8695997 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -24,7 +24,7 @@ jobs: strategy: matrix: NXF_VER: - - "23.10.1" + - "23.04.0" - "latest-everything" ANALYSIS: - "test_screening" diff --git a/README.md b/README.md index 1b0dceed..cd3e0b78 100644 --- a/README.md +++ b/README.md @@ -9,7 +9,7 @@ [![GitHub Actions Linting Status](https://github.com/nf-core/crisprseq/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/crisprseq/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/crisprseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.7598496-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.7598496) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.10.1-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) diff --git a/nextflow.config b/nextflow.config index b2fb9a86..a6c8003a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -276,7 +276,7 @@ manifest { homePage = 'https://github.com/nf-core/crisprseq' description = """Pipeline for the analysis of CRISPR data""" mainScript = 'main.nf' - nextflowVersion = '!>=23.10.1' + nextflowVersion = '!>=23.04.0' version = '2.2.0dev' doi = 'https://doi.org/10.5281/zenodo.7598496' } From d2267c0ee101cf39ce263086ac97f1eadb36bd30 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 3 May 2024 11:31:18 +0200 Subject: [PATCH 145/226] update version --- .../nf-core/utils_nextflow_pipeline/tests/nextflow.config | 2 +- .../nf-core/utils_nfcore_pipeline/tests/nextflow.config | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index 8f545add..d0a926bf 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -3,7 +3,7 @@ manifest { author = """nf-core""" homePage = 'https://127.0.0.1' description = """Dummy pipeline""" - nextflowVersion = '!>=23.10.1' + nextflowVersion = '!>=23.04.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' } diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config index 8f545add..d0a926bf 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -3,7 +3,7 @@ manifest { author = """nf-core""" homePage = 'https://127.0.0.1' description = """Dummy pipeline""" - nextflowVersion = '!>=23.10.1' + nextflowVersion = '!>=23.04.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' } From 7c90afe4d76ba6fa357d59cb46718fb98f84d51c Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 3 May 2024 12:46:05 +0200 Subject: [PATCH 146/226] Bump version --- CHANGELOG.md | 2 +- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 3 files changed, 4 insertions(+), 4 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 9450e48d..cd0741bf 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## [v2.2.0dev] +## [v3.0.0 - Romarin Curie] ### Added diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index da1da3e0..03089144 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/crisprseq + This report has been generated by the nf-core/crisprseq analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-crisprseq-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index a6c8003a..b85a106b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -277,7 +277,7 @@ manifest { description = """Pipeline for the analysis of CRISPR data""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '2.2.0dev' + version = '3.0.0' doi = 'https://doi.org/10.5281/zenodo.7598496' } From 92cc856a4827a8466568c0fc2ae5523b46384c81 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 3 May 2024 12:52:49 +0200 Subject: [PATCH 147/226] bump to v2.2.0 --- CHANGELOG.md | 2 +- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 3 files changed, 4 insertions(+), 4 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index cd0741bf..c8e1b3aa 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## [v3.0.0 - Romarin Curie] +## [v2.2.0 - Romarin Curie] ### Added diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 03089144..3c60e074 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/crisprseq + This report has been generated by the nf-core/crisprseq analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-crisprseq-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index b85a106b..d098530c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -277,7 +277,7 @@ manifest { description = """Pipeline for the analysis of CRISPR data""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '3.0.0' + version = '2.2.0' doi = 'https://doi.org/10.5281/zenodo.7598496' } From 6e99a3b926819c0d9f6afacf4721d8dbe30efe34 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Mon, 6 May 2024 11:57:00 +0200 Subject: [PATCH 148/226] Update github action to the same in nf-core tools --- .github/workflows/download_pipeline.yml | 4 ++-- workflows/crisprseq_screening.nf | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 08622fd5..7d0279c3 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -30,9 +30,9 @@ jobs: - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: "3.11" + python-version: "3.12" architecture: "x64" - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 8d5831da..14d62be1 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -269,7 +269,7 @@ workflow CRISPRSEQ_SCREENING { ch_mle = ch_designed_mle.combine(ch_counts) MAGECK_MLE_MATRIX (ch_mle) ch_versions = ch_versions.mix(MAGECK_MLE_MATRIX.out.versions) - MAGECK_FLUTEMLE(MAGECK_MLE.out.gene_summary) + MAGECK_FLUTEMLE(MAGECK_MLE_MATRIX.out.gene_summary) ch_versions = ch_versions.mix(MAGECK_FLUTEMLE.out.versions) } if(params.contrasts) { From e247ed8a0cdd6951b9abaaa5c35030bf01d313fa Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Tue, 7 May 2024 12:30:36 +0200 Subject: [PATCH 149/226] create empty -QC-indels.csv file if alignments not found - fix AWS megatests --- bin/cigar_parser.R | 6 ++++++ 1 file changed, 6 insertions(+) diff --git a/bin/cigar_parser.R b/bin/cigar_parser.R index 2018d686..cace0d1c 100755 --- a/bin/cigar_parser.R +++ b/bin/cigar_parser.R @@ -1234,4 +1234,10 @@ if (dim(alignment_info)[1] != 0){ colnames(edit_summary_perc)[1] = results_path # Rename the column to add the sample ID edit_summary_perc <- t(edit_summary_perc) # t() will add classes as columns and counts as values, 1 row per sample write.csv(edit_summary_perc,file=paste0(results_path, "_edits.csv")) + indel_filters + prevc_classes_mqc <- c("Wt passing filter", "Wt NOT passing filter", "Indels NOT passing filter", + "Above error & in pick", "NOT above error & in pick", "NOT above error & NOT in pick", "Above error & NOT in pick") + prevc_counts_mqc <- c(0, 0, 0, 0, 0, 0, 0) + indel_filters <- data.frame(sample = unlist(prevc_counts_mqc), row.names = unlist(prevc_classes_mqc)) + write.csv(indel_filters,file=paste0(results_path, "_QC-indels.csv")) } From 13c792f758963d53ac4d0579ee9d98de8aa46b7a Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Tue, 7 May 2024 12:37:17 +0200 Subject: [PATCH 150/226] udpate changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index c8e1b3aa..7f02f834 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -19,6 +19,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Fixed - Adapt cutadapt module to work with single-end and paired-end reads again ([#121](https://github.com/nf-core/crisprseq/pull/121)) +- Create empty *-QC-indels.csv file if alignments not found. ([#138](https://github.com/nf-core/crisprseq/pull/138)) ## [v2.1.1 - Jamon Salas - patch](https://github.com/nf-core/crisprseq/releases/tag/2.1.1) - [14.12.2023] From e0c3e2d0173a04ecce80816e65b7741e15bb9ccf Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 7 May 2024 10:51:19 +0000 Subject: [PATCH 151/226] [automated] Fix linting with Prettier --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 7f02f834..44224ef2 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -19,7 +19,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Fixed - Adapt cutadapt module to work with single-end and paired-end reads again ([#121](https://github.com/nf-core/crisprseq/pull/121)) -- Create empty *-QC-indels.csv file if alignments not found. ([#138](https://github.com/nf-core/crisprseq/pull/138)) +- Create empty \*-QC-indels.csv file if alignments not found. ([#138](https://github.com/nf-core/crisprseq/pull/138)) ## [v2.1.1 - Jamon Salas - patch](https://github.com/nf-core/crisprseq/releases/tag/2.1.1) - [14.12.2023] From 589a7dad223e0f693641667b185b62aec51ce6cf Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 8 May 2024 13:58:06 +0000 Subject: [PATCH 152/226] Template update for nf-core/tools version 2.14.0 --- .editorconfig | 6 +- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/awsfulltest.yml | 10 +- .github/workflows/awstest.yml | 12 +- .github/workflows/ci.yml | 4 +- .github/workflows/download_pipeline.yml | 22 ++- .github/workflows/fix-linting.yml | 6 +- .github/workflows/linting.yml | 18 +- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 6 +- .nf-core.yml | 1 + .pre-commit-config.yaml | 3 + CHANGELOG.md | 2 +- README.md | 2 +- assets/multiqc_config.yml | 6 +- conf/base.config | 3 - conf/modules.config | 8 - conf/test.config | 2 +- conf/test_full.config | 2 +- docs/usage.md | 2 + modules.json | 4 +- modules/nf-core/fastqc/main.nf | 6 + nextflow.config | 178 +++++++++--------- nextflow_schema.json | 7 + pyproject.toml | 15 -- .../utils_nfcore_crisprseq_pipeline/main.nf | 16 +- .../nf-core/utils_nfcore_pipeline/main.nf | 8 +- workflows/crisprseq.nf | 46 +++-- 28 files changed, 226 insertions(+), 173 deletions(-) delete mode 100644 pyproject.toml diff --git a/.editorconfig b/.editorconfig index dd9ffa53..72dda289 100644 --- a/.editorconfig +++ b/.editorconfig @@ -28,10 +28,6 @@ indent_style = unset [/assets/email*] indent_size = unset -# ignore Readme -[README.md] -indent_style = unset - -# ignore python +# ignore python and markdown [*.{py,md}] indent_style = unset diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index c6709972..1ae44009 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/cris - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/crisprseq/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/crisprseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). +- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index b393817c..9ffd0f54 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -8,12 +8,12 @@ on: types: [published] workflow_dispatch: jobs: - run-tower: + run-platform: name: Run AWS full tests if: github.repository == 'nf-core/crisprseq' runs-on: ubuntu-latest steps: - - name: Launch workflow via tower + - name: Launch workflow via Seqera Platform uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) @@ -33,7 +33,7 @@ jobs: - uses: actions/upload-artifact@v4 with: - name: Tower debug log file + name: Seqera Platform debug log file path: | - tower_action_*.log - tower_action_*.json + seqera_platform_action_*.log + seqera_platform_action_*.json diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index f46f773a..53bbbc4f 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -5,13 +5,13 @@ name: nf-core AWS test on: workflow_dispatch: jobs: - run-tower: + run-platform: name: Run AWS tests if: github.repository == 'nf-core/crisprseq' runs-on: ubuntu-latest steps: - # Launch workflow using Tower CLI tool action - - name: Launch workflow via tower + # Launch workflow using Seqera Platform CLI tool action + - name: Launch workflow via Seqera Platform uses: seqeralabs/action-tower-launch@v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} @@ -27,7 +27,7 @@ jobs: - uses: actions/upload-artifact@v4 with: - name: Tower debug log file + name: Seqera Platform debug log file path: | - tower_action_*.log - tower_action_*.json + seqera_platform_action_*.log + seqera_platform_action_*.json diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index f7646db6..2ac31dcb 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,10 +28,10 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 with: version: "${{ matrix.NXF_VER }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 08622fd5..2d20d644 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -14,6 +14,8 @@ on: pull_request: types: - opened + - edited + - synchronize branches: - master pull_request_target: @@ -28,11 +30,14 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: "3.11" + python-version: "3.12" architecture: "x64" - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: @@ -65,8 +70,17 @@ jobs: - name: Inspect download run: tree ./${{ env.REPOTITLE_LOWERCASE }} - - name: Run the downloaded pipeline + - name: Run the downloaded pipeline (stub) + id: stub_run_pipeline + continue-on-error: true env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + - name: Run the downloaded pipeline (stub run not supported) + id: run_pipeline + if: ${{ job.steps.stub_run_pipeline.status == failure() }} + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 9fc36a6c..a9ddbe9d 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -13,7 +13,7 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} @@ -32,9 +32,9 @@ jobs: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} # Install and run pre-commit - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: 3.11 + python-version: "3.12" - name: Install pre-commit run: pip install pre-commit diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 073e1876..a3fb2541 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,12 +14,12 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - - name: Set up Python 3.11 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - name: Set up Python 3.12 + uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: 3.11 + python-version: "3.12" cache: "pip" - name: Install pre-commit @@ -32,14 +32,14 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: "3.11" + python-version: "3.12" architecture: "x64" - name: Install dependencies @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index b706875f..40acc23f 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 + uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index d468aeaa..03ecfcf7 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -12,7 +12,7 @@ jobs: - name: get topics and convert to hashtags id: get_topics run: | - curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT + echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" >> $GITHUB_OUTPUT - uses: rzr/fediverse-action@master with: @@ -25,13 +25,13 @@ jobs: Please see the changelog: ${{ github.event.release.html_url }} - ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics + ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics send-tweet: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: python-version: "3.10" - name: Install dependencies diff --git a/.nf-core.yml b/.nf-core.yml index 3805dc81..d6daa403 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1 +1,2 @@ repository_type: pipeline +nf_core_version: "2.14.0" diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index af57081f..4dc0f1dc 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -3,6 +3,9 @@ repos: rev: "v3.1.0" hooks: - id: prettier + additional_dependencies: + - prettier@3.2.5 + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python rev: "2.7.3" hooks: diff --git a/CHANGELOG.md b/CHANGELOG.md index 3b744cbd..5c932e79 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.2.0dev - [date] +## v2.2.0 - [date] Initial release of nf-core/crisprseq, created with the [nf-core](https://nf-co.re/) template. diff --git a/README.md b/README.md index f5ad96c3..0ad03b72 100644 --- a/README.md +++ b/README.md @@ -13,7 +13,7 @@ [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/crisprseq) +[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/crisprseq) [![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23crisprseq-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/crisprseq)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index e500d031..56227af8 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,9 @@ report_comment: > - This report has been generated by the nf-core/crisprseq + + This report has been generated by the nf-core/crisprseq analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. + report_section_order: "nf-core-crisprseq-methods-description": order: -1000 diff --git a/conf/base.config b/conf/base.config index c2474343..6198f4f8 100644 --- a/conf/base.config +++ b/conf/base.config @@ -59,7 +59,4 @@ process { errorStrategy = 'retry' maxRetries = 2 } - withName:CUSTOM_DUMPSOFTWAREVERSIONS { - cache = false - } } diff --git a/conf/modules.config b/conf/modules.config index e3ea8fa6..d203d2b6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -22,14 +22,6 @@ process { ext.args = '--quiet' } - withName: CUSTOM_DUMPSOFTWAREVERSIONS { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - pattern: '*_versions.yml' - ] - } - withName: 'MULTIQC' { ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ diff --git a/conf/test.config b/conf/test.config index 5b26eb39..4747af85 100644 --- a/conf/test.config +++ b/conf/test.config @@ -22,7 +22,7 @@ params { // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' // Genome references genome = 'R64-1-1' diff --git a/conf/test_full.config b/conf/test_full.config index 85bc453c..44a536f0 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -17,7 +17,7 @@ params { // Input data for full size test // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' + input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' // Genome references genome = 'R64-1-1' diff --git a/docs/usage.md b/docs/usage.md index 2247d5ba..100156b1 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -156,6 +156,8 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) - `apptainer` - A generic configuration profile to be used with [Apptainer](https://apptainer.org/) +- `wave` + - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. Use together with one of the above (requires Nextflow ` 24.03.0-edge` or later). - `conda` - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer. diff --git a/modules.json b/modules.json index 609fe705..79a99b94 100644 --- a/modules.json +++ b/modules.json @@ -7,7 +7,7 @@ "nf-core": { "fastqc": { "branch": "master", - "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", + "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", "installed_by": ["modules"] }, "multiqc": { @@ -26,7 +26,7 @@ }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 9e19a74c..d79f1c86 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -25,6 +25,11 @@ process FASTQC { def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } def rename_to = old_new_pairs*.join(' ').join(' ') def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') + + def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') + // FastQC memory value allowed range (100 - 10000) + def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb) + """ printf "%s %s\\n" $rename_to | while read old_name new_name; do [ -f "\${new_name}" ] || ln -s \$old_name \$new_name @@ -33,6 +38,7 @@ process FASTQC { fastqc \\ $args \\ --threads $task.cpus \\ + --memory $fastqc_memory \\ $renamed_files cat <<-END_VERSIONS > versions.yml diff --git a/nextflow.config b/nextflow.config index 817d2a71..5a1f1ed2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,7 +16,8 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - fasta = null// MultiQC options + + // MultiQC options multiqc_config = null multiqc_title = null multiqc_logo = null @@ -24,15 +25,16 @@ params { multiqc_methods_description = null // Boilerplate options - outdir = null - publish_dir_mode = 'copy' - email = null - email_on_fail = null - plaintext_email = false - monochrome_logs = false - hook_url = null - help = false - version = false + outdir = null + publish_dir_mode = 'copy' + email = null + email_on_fail = null + plaintext_email = false + monochrome_logs = false + hook_url = null + help = false + version = false + pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' // Config options config_profile_name = null @@ -68,103 +70,109 @@ try { } // Load nf-core/crisprseq custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! -// try { -// includeConfig "${params.custom_config_base}/pipeline/crisprseq.config" -// } catch (Exception e) { -// System.err.println("WARNING: Could not load nf-core/config/crisprseq profiles: ${params.custom_config_base}/pipeline/crisprseq.config") -// } +try { + includeConfig "${params.custom_config_base}/pipeline/crisprseq.config" +} catch (Exception e) { + System.err.println("WARNING: Could not load nf-core/config/crisprseq profiles: ${params.custom_config_base}/pipeline/crisprseq.config") +} profiles { debug { - dumpHashes = true - process.beforeScript = 'echo $HOSTNAME' - cleanup = false + dumpHashes = true + process.beforeScript = 'echo $HOSTNAME' + cleanup = false nextflow.enable.configProcessNamesValidation = true } conda { - conda.enabled = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - channels = ['conda-forge', 'bioconda', 'defaults'] - apptainer.enabled = false + conda.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + conda.channels = ['conda-forge', 'bioconda', 'defaults'] + apptainer.enabled = false } mamba { - conda.enabled = true - conda.useMamba = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + conda.enabled = true + conda.useMamba = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } docker { - docker.enabled = true - conda.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false - docker.runOptions = '-u $(id -u):$(id -g)' + docker.enabled = true + conda.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { - singularity.enabled = true - singularity.autoMounts = true - conda.enabled = false - docker.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + singularity.enabled = true + singularity.autoMounts = true + conda.enabled = false + docker.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } podman { - podman.enabled = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + podman.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } shifter { - shifter.enabled = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - podman.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + shifter.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } charliecloud { - charliecloud.enabled = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - apptainer.enabled = false + charliecloud.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + apptainer.enabled = false } apptainer { - apptainer.enabled = true - apptainer.autoMounts = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false + apptainer.enabled = true + apptainer.autoMounts = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + wave { + apptainer.ociAutoPull = true + singularity.ociAutoPull = true + wave.enabled = true + wave.freeze = true + wave.strategy = 'conda,container' } gitpod { - executor.name = 'local' - executor.cpus = 4 - executor.memory = 8.GB + executor.name = 'local' + executor.cpus = 4 + executor.memory = 8.GB } test { includeConfig 'conf/test.config' } test_full { includeConfig 'conf/test_full.config' } @@ -231,7 +239,7 @@ manifest { description = """Pipeline for the analysis of CRISPR data""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '2.2.0dev' + version = '2.2.0' doi = '' } diff --git a/nextflow_schema.json b/nextflow_schema.json index bfea1e1d..20cad0ca 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -265,6 +265,13 @@ "description": "Validation of parameters in lenient more.", "hidden": true, "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." + }, + "pipelines_testdata_base_path": { + "type": "string", + "fa_icon": "far fa-check-circle", + "description": "Base URL or local path to location of pipeline test dataset files", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/", + "hidden": true } } } diff --git a/pyproject.toml b/pyproject.toml deleted file mode 100644 index 56110621..00000000 --- a/pyproject.toml +++ /dev/null @@ -1,15 +0,0 @@ -# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. -# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.ruff] -line-length = 120 -target-version = "py38" -cache-dir = "~/.cache/ruff" - -[tool.ruff.lint] -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] - -[tool.ruff.lint.isort] -known-first-party = ["nf_core"] - -[tool.ruff.lint.per-file-ignores] -"__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index 42992053..4da12227 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -140,6 +140,10 @@ workflow PIPELINE_COMPLETION { imNotification(summary_params, hook_url) } } + + workflow.onError { + log.error "Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting" + } } /* @@ -230,8 +234,16 @@ def methodsDescriptionText(mqc_methods_yaml) { meta["manifest_map"] = workflow.manifest.toMap() // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "

  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + if (meta.manifest_map.doi) { + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + def temp_doi_ref = "" + String[] manifest_doi = meta.manifest_map.doi.tokenize(",") + for (String doi_ref: manifest_doi) temp_doi_ref += "(doi: ${doi_ref.replace("https://doi.org/", "").replace(" ", "")}), " + meta["doi_text"] = temp_doi_ref.substring(0, temp_doi_ref.length() - 2) + } else meta["doi_text"] = "" + meta["nodoi_text"] = meta.manifest_map.doi ? "" : "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " // Tool references meta["tool_citations"] = "" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index a8b55d6f..14558c39 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -65,9 +65,15 @@ def checkProfileProvided(nextflow_cli_args) { // Citation string for pipeline // def workflowCitation() { + def temp_doi_ref = "" + String[] manifest_doi = workflow.manifest.doi.tokenize(",") + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + for (String doi_ref: manifest_doi) temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + - " ${workflow.manifest.doi}\n\n" + + temp_doi_ref + "\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + diff --git a/workflows/crisprseq.nf b/workflows/crisprseq.nf index 467da216..3ee6cfd5 100644 --- a/workflows/crisprseq.nf +++ b/workflows/crisprseq.nf @@ -40,22 +40,44 @@ workflow CRISPRSEQ { // Collate and save software versions // softwareVersionsToYAML(ch_versions) - .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) - .set { ch_collated_versions } + .collectFile( + storeDir: "${params.outdir}/pipeline_info", + name: 'nf_core_pipeline_software_mqc_versions.yml', + sort: true, + newLine: true + ).set { ch_collated_versions } // // MODULE: MultiQC // - ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) - ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() - ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() - summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") - ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) - ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) + ch_multiqc_config = Channel.fromPath( + "$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? + Channel.fromPath(params.multiqc_config, checkIfExists: true) : + Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? + Channel.fromPath(params.multiqc_logo, checkIfExists: true) : + Channel.empty() + + summary_params = paramsSummaryMap( + workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? + file(params.multiqc_methods_description, checkIfExists: true) : + file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value( + methodsDescriptionText(ch_multiqc_custom_methods_description)) + + ch_multiqc_files = ch_multiqc_files.mix( + ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix( + ch_methods_description.collectFile( + name: 'methods_description_mqc.yaml', + sort: true + ) + ) MULTIQC ( ch_multiqc_files.collect(), From 87b68e01b62f9172ca7dc2ccf3eb1d81c8e8d11e Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 9 May 2024 11:40:13 +0000 Subject: [PATCH 153/226] Template update for nf-core/tools version 2.14.1 --- .github/workflows/linting.yml | 1 - .nf-core.yml | 2 +- 2 files changed, 1 insertion(+), 2 deletions(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index a3fb2541..1fcafe88 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -20,7 +20,6 @@ jobs: uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: python-version: "3.12" - cache: "pip" - name: Install pre-commit run: pip install pre-commit diff --git a/.nf-core.yml b/.nf-core.yml index d6daa403..e0b85a77 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,2 +1,2 @@ repository_type: pipeline -nf_core_version: "2.14.0" +nf_core_version: "2.14.1" From d464da049d2e63e14d24a21670e566f322772d6e Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Fri, 17 May 2024 07:37:50 +0200 Subject: [PATCH 154/226] Update download_pipeline.yml --- .github/workflows/download_pipeline.yml | 18 +++++++++++++++--- 1 file changed, 15 insertions(+), 3 deletions(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 7d0279c3..7cc749f5 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -28,7 +28,10 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 + + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: @@ -64,9 +67,18 @@ jobs: - name: Inspect download run: tree ./${{ env.REPOTITLE_LOWERCASE }} - - - name: Run the downloaded pipeline + - name: Run the downloaded pipeline (stub) + id: stub_run_pipeline + continue-on-error: true env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + + - name: Run the downloaded pipeline (stub run not supported) + id: run_pipeline + if: ${{ job.steps.stub_run_pipeline.status == failure() }} + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results{% endraw %} From 44913f450a50e3513796cbf4ed0f2193b0682b3e Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 09:01:39 +0200 Subject: [PATCH 155/226] Ran pre commit --- .github/workflows/download_pipeline.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 7cc749f5..6dad1232 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -74,7 +74,7 @@ jobs: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results - + - name: Run the downloaded pipeline (stub run not supported) id: run_pipeline if: ${{ job.steps.stub_run_pipeline.status == failure() }} From 97ffcd5aa25054e87038ae043b25d91ee5a47666 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 09:43:06 +0200 Subject: [PATCH 156/226] Add test for count table --- .github/workflows/ci.yml | 1 + conf/test_screening_count_table.config | 29 ++++++++++++++++++++++++++ nextflow.config | 19 +++++++++-------- 3 files changed, 40 insertions(+), 9 deletions(-) create mode 100644 conf/test_screening_count_table.config diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index e8695997..304320ab 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -32,6 +32,7 @@ jobs: - "test_screening_rra" - "test_targeted" - "test_umis" + - "test_screening_count_table" steps: - name: Check out pipeline code diff --git a/conf/test_screening_count_table.config b/conf/test_screening_count_table.config new file mode 100644 index 00000000..0a1f55da --- /dev/null +++ b/conf/test_screening_count_table.config @@ -0,0 +1,29 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/crisprseq -profile test_screening, --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test screening profile with an input count table' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' + + // Input data + count_table = params.pipelines_testdata_base_path + 'crisprseq/testdata/count_table.csv' + analysis = 'screening' + crisprcleanr = "Brunello_Library" + mle_design_matrix = params.pipelines_testdata_base_path + "crisprseq/testdata/design_matrix.txt" + contrasts = params.pipelines_testdata_base_path + "crisprseq/testdata/rra_contrasts.txt" + +} diff --git a/nextflow.config b/nextflow.config index d098530c..d2e1b69d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -197,15 +197,16 @@ profiles { executor.cpus = 4 executor.memory = 8.GB } - test { includeConfig 'conf/test_targeted.config' } - test_targeted { includeConfig 'conf/test_targeted.config' } - test_full { includeConfig 'conf/test_targeted_full.config' } - test_targeted_full { includeConfig 'conf/test_targeted_full.config' } - test_umis { includeConfig 'conf/test_umis.config' } - test_screening_full { includeConfig 'conf/test_screening_full.config' } - test_screening { includeConfig 'conf/test_screening.config' } - test_screening_paired { includeConfig 'conf/test_screening_paired.config' } - test_screening_rra { includeConfig 'conf/test_screening_rra.config' } + test { includeConfig 'conf/test_targeted.config' } + test_targeted { includeConfig 'conf/test_targeted.config' } + test_full { includeConfig 'conf/test_targeted_full.config' } + test_targeted_full { includeConfig 'conf/test_targeted_full.config' } + test_umis { includeConfig 'conf/test_umis.config' } + test_screening_full { includeConfig 'conf/test_screening_full.config' } + test_screening { includeConfig 'conf/test_screening.config' } + test_screening_paired { includeConfig 'conf/test_screening_paired.config' } + test_screening_rra { includeConfig 'conf/test_screening_rra.config' } + test_screening_count_table { includeConfig 'conf/test_screening_count_table.config' } } // Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile From 2558ab97ce18eee91c6256a74377e18e44e6a017 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 10:04:14 +0200 Subject: [PATCH 157/226] Fix path --- conf/test_screening_count_table.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/test_screening_count_table.config b/conf/test_screening_count_table.config index 0a1f55da..2f7ff351 100644 --- a/conf/test_screening_count_table.config +++ b/conf/test_screening_count_table.config @@ -23,7 +23,7 @@ params { count_table = params.pipelines_testdata_base_path + 'crisprseq/testdata/count_table.csv' analysis = 'screening' crisprcleanr = "Brunello_Library" - mle_design_matrix = params.pipelines_testdata_base_path + "crisprseq/testdata/design_matrix.txt" - contrasts = params.pipelines_testdata_base_path + "crisprseq/testdata/rra_contrasts.txt" + mle_design_matrix = params.pipelines_testdata_base_path + 'crisprseq/testdata/design_matrix.txt' + contrasts = params.pipelines_testdata_base_path + 'crisprseq/testdata/rra_contrasts.txt' } From 48b834d8f71c8cea5db38e838485f650007965e8 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 10:16:29 +0200 Subject: [PATCH 158/226] Revert to the old nomenclature for now --- conf/test_screening_count_table.config | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/conf/test_screening_count_table.config b/conf/test_screening_count_table.config index 2f7ff351..d6a9c5cc 100644 --- a/conf/test_screening_count_table.config +++ b/conf/test_screening_count_table.config @@ -20,10 +20,10 @@ params { max_time = '6.h' // Input data - count_table = params.pipelines_testdata_base_path + 'crisprseq/testdata/count_table.csv' + count_table = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/count_table.csv" analysis = 'screening' crisprcleanr = "Brunello_Library" - mle_design_matrix = params.pipelines_testdata_base_path + 'crisprseq/testdata/design_matrix.txt' - contrasts = params.pipelines_testdata_base_path + 'crisprseq/testdata/rra_contrasts.txt' + mle_design_matrix = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/design_matrix.txt" + contrasts = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/rra_contrasts.txt" } From 01b70571b1ef87c00c7d53d78bbfa2c3520f13b3 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 10:35:51 +0200 Subject: [PATCH 159/226] Temporarily use my path until i fix the github issue --- conf/test_screening_count_table.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_screening_count_table.config b/conf/test_screening_count_table.config index d6a9c5cc..1797763f 100644 --- a/conf/test_screening_count_table.config +++ b/conf/test_screening_count_table.config @@ -20,7 +20,7 @@ params { max_time = '6.h' // Input data - count_table = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/count_table.csv" + count_table = "https://raw.githubusercontent.com/LaurenceKuhl/test-datasets/crisprseq/testdata/count_table.tsv" analysis = 'screening' crisprcleanr = "Brunello_Library" mle_design_matrix = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/design_matrix.txt" From bd49779e0b7f70677711cc28be038ebac5dd1b0a Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 13:15:08 +0200 Subject: [PATCH 160/226] Current tries to make the input non mandatory --- .../utils_nfcore_crisprseq_pipeline/main.nf | 48 +++++++++++-------- 1 file changed, 27 insertions(+), 21 deletions(-) diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index 4929ab09..309bc4df 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -80,32 +80,38 @@ workflow PIPELINE_INITIALISATION { // // Create channel from input file provided through params.input // - Channel - .fromSamplesheet("input") - .multiMap { - meta, fastq_1, fastq_2, reference, protospacer, template -> - if (fastq_2) { - files = [ fastq_1, fastq_2 ] - } else { - files = [ fastq_1 ] + if(params.input) { + Channel + .fromSamplesheet("input") + .multiMap { + meta, fastq_1, fastq_2, reference, protospacer, template -> + if (fastq_2) { + files = [ fastq_1, fastq_2 ] + } else { + files = [ fastq_1 ] + } + reads_targeted: [ meta.id, meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], files ] + reads_screening:[ meta + [ single_end:fastq_2?false:true ], files ] + reference: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], reference] + protospacer: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], protospacer] + template: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], template] + } + .set { ch_input } + } else { + ch_input = Channel.empty() } - reads_targeted: [ meta.id, meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], files ] - reads_screening:[ meta + [ single_end:fastq_2?false:true ], files ] - reference: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], reference] - protospacer: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], protospacer] - template: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], template] - } - .set { ch_input } // // Validate input samplesheet // - ch_input.reads_targeted - .groupTuple() - .map { - validateInputSamplesheet(it) - } - .set { reads_targeted } + if(params.input) { + ch_input.reads_targeted + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .set { reads_targeted } + } emit: reads_targeted = reads_targeted From 481a0bb38dc074cb855cc6d9b45986fc51a5e141 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 13:26:35 +0200 Subject: [PATCH 161/226] Fix curly brackets --- .../utils_nfcore_crisprseq_pipeline/main.nf | 75 +++++++++++++------ 1 file changed, 54 insertions(+), 21 deletions(-) diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index 309bc4df..01d4e380 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -83,35 +83,68 @@ workflow PIPELINE_INITIALISATION { if(params.input) { Channel .fromSamplesheet("input") - .multiMap { - meta, fastq_1, fastq_2, reference, protospacer, template -> - if (fastq_2) { - files = [ fastq_1, fastq_2 ] - } else { - files = [ fastq_1 ] - } - reads_targeted: [ meta.id, meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], files ] - reads_screening:[ meta + [ single_end:fastq_2?false:true ], files ] - reference: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], reference] - protospacer: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], protospacer] - template: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], template] + .multiMap { meta, fastq_1, fastq_2, reference, protospacer, template -> + def files = fastq_2 ? [ fastq_1, fastq_2 ] : [ fastq_1 ] + + [ + reads_targeted: [ + meta.id, + meta - meta.subMap('condition') + [ + single_end: !fastq_2, + self_reference: !reference, + template: template + ], + files + ], + reads_screening: [ + meta + [ + single_end: !fastq_2 + ], + files + ], + reference: [ + meta - meta.subMap('condition') + [ + single_end: !fastq_2, + self_reference: !reference, + template: template + ], + reference + ], + protospacer: [ + meta - meta.subMap('condition') + [ + single_end: !fastq_2, + self_reference: !reference, + template: template + ], + protospacer + ], + template: [ + meta - meta.subMap('condition') + [ + single_end: !fastq_2, + self_reference: !reference, + template: template + ], + template + ] + ] } .set { ch_input } - } else { - ch_input = Channel.empty() - } + } else { + ch_input = Channel.empty() + } + // // Validate input samplesheet // - if(params.input) { + if (params.input) { ch_input.reads_targeted - .groupTuple() - .map { - validateInputSamplesheet(it) - } + .groupTuple() + .map { + validateInputSamplesheet(it) + } .set { reads_targeted } - } + } emit: reads_targeted = reads_targeted From 43bd503f9a6e164e29fb05fad7074d9d7903be85 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 13:28:57 +0200 Subject: [PATCH 162/226] Fix curly brackets --- subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index 01d4e380..bae54fdb 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -129,9 +129,9 @@ workflow PIPELINE_INITIALISATION { ] } .set { ch_input } - } else { - ch_input = Channel.empty() - } + } else { + ch_input = Channel.empty() + } // From a065a28d964d6eb5d11c3325ddc1ad4fbb5c104a Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 13:38:19 +0200 Subject: [PATCH 163/226] fix curly brackets.. --- .../utils_nfcore_crisprseq_pipeline/main.nf | 69 +++++-------------- 1 file changed, 18 insertions(+), 51 deletions(-) diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index bae54fdb..eedd2193 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -83,66 +83,33 @@ workflow PIPELINE_INITIALISATION { if(params.input) { Channel .fromSamplesheet("input") - .multiMap { meta, fastq_1, fastq_2, reference, protospacer, template -> - def files = fastq_2 ? [ fastq_1, fastq_2 ] : [ fastq_1 ] - - [ - reads_targeted: [ - meta.id, - meta - meta.subMap('condition') + [ - single_end: !fastq_2, - self_reference: !reference, - template: template - ], - files - ], - reads_screening: [ - meta + [ - single_end: !fastq_2 - ], - files - ], - reference: [ - meta - meta.subMap('condition') + [ - single_end: !fastq_2, - self_reference: !reference, - template: template - ], - reference - ], - protospacer: [ - meta - meta.subMap('condition') + [ - single_end: !fastq_2, - self_reference: !reference, - template: template - ], - protospacer - ], - template: [ - meta - meta.subMap('condition') + [ - single_end: !fastq_2, - self_reference: !reference, - template: template - ], - template - ] - ] + .multiMap { + meta, fastq_1, fastq_2, reference, protospacer, template -> + if (fastq_2) { + files = [ fastq_1, fastq_2 ] + } else { + files = [ fastq_1 ] + } + reads_targeted: [ meta.id, meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], files ] + reads_screening:[ meta + [ single_end:fastq_2?false:true ], files ] + reference: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], reference] + protospacer: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], protospacer] + template: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], template] } .set { ch_input } } else { - ch_input = Channel.empty() + ch_input = Channel.empty() } - // // Validate input samplesheet // - if (params.input) { + if(params.input) { ch_input.reads_targeted - .groupTuple() - .map { - validateInputSamplesheet(it) - } + .groupTuple() + .map { + validateInputSamplesheet(it) + } .set { reads_targeted } } From e9acfb1e34b569fe47c0944ce1e3b716874b271b Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 13:40:32 +0200 Subject: [PATCH 164/226] Trying to add empty channels --- subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf | 2 ++ 1 file changed, 2 insertions(+) diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index eedd2193..fa06ff8c 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -111,6 +111,8 @@ workflow PIPELINE_INITIALISATION { validateInputSamplesheet(it) } .set { reads_targeted } + } else { + reads_targeted = Channel.empty() } emit: From 676a9407a3c4584fd784dce3078b8a2240385c38 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 16:02:54 +0200 Subject: [PATCH 165/226] Fix input count file --- conf/test_screening_count_table.config | 1 - .../utils_nfcore_crisprseq_pipeline/main.nf | 25 +++++++++++++++---- templates/template_fluteMLE.R | 7 +++++- workflows/crisprseq_screening.nf | 10 +++++--- 4 files changed, 32 insertions(+), 11 deletions(-) diff --git a/conf/test_screening_count_table.config b/conf/test_screening_count_table.config index 1797763f..ef6b8442 100644 --- a/conf/test_screening_count_table.config +++ b/conf/test_screening_count_table.config @@ -22,7 +22,6 @@ params { // Input data count_table = "https://raw.githubusercontent.com/LaurenceKuhl/test-datasets/crisprseq/testdata/count_table.tsv" analysis = 'screening' - crisprcleanr = "Brunello_Library" mle_design_matrix = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/design_matrix.txt" contrasts = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/rra_contrasts.txt" diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index fa06ff8c..e52bbb14 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -77,6 +77,16 @@ workflow PIPELINE_INITIALISATION { // validateInputParameters() + + + reads_targeted = Channel.empty() + reads_screening = Channel.empty() + fastqc_screening = Channel.empty() + reference = Channel.empty() + protospacer = Channel.empty() + template = Channel.empty() + versions = Channel.empty() + // // Create channel from input file provided through params.input // @@ -97,6 +107,11 @@ workflow PIPELINE_INITIALISATION { template: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], template] } .set { ch_input } + fastqc_screening = ch_input.reads_screening + fastqc_screening = ch_input.reads_screening + reference = ch_input.reference + protospacer = ch_input.protospacer + template = ch_input.template } else { ch_input = Channel.empty() } @@ -116,11 +131,11 @@ workflow PIPELINE_INITIALISATION { } emit: - reads_targeted = reads_targeted - fastqc_screening = ch_input.reads_screening - reference = ch_input.reference - protospacer = ch_input.protospacer - template = ch_input.template + reads_targeted + fastqc_screening + reference + protospacer + template versions = ch_versions } diff --git a/templates/template_fluteMLE.R b/templates/template_fluteMLE.R index b942c898..1a9c10fd 100644 --- a/templates/template_fluteMLE.R +++ b/templates/template_fluteMLE.R @@ -20,7 +20,12 @@ FluteMLE(mle, treatname= i, proj=i, pathview.top=5) } } else { - FluteMLE(mle, treatname= "${prefix}", proj="${prefix}", ${args}, pathview.top=5) + beta_strings <- grep("\\\\.beta", colnames(mle), value = TRUE) + before_beta <- sub("\\\\.beta.*", "", beta_strings) + unique_strings <- unique(before_beta) + for(i in unique_strings) { + FluteMLE(mle, treatname= i, proj=i, ${args}, pathview.top=5) + } } version_file_path <- "versions.yml" diff --git a/workflows/crisprseq_screening.nf b/workflows/crisprseq_screening.nf index 14d62be1..5ccb9310 100644 --- a/workflows/crisprseq_screening.nf +++ b/workflows/crisprseq_screening.nf @@ -11,6 +11,8 @@ include { BAGEL2_PR } from '../modules/local/ include { BAGEL2_GRAPH } from '../modules/local/bagel2/graph' include { MATRICESCREATION } from '../modules/local/matricescreation' include { MAGECK_FLUTEMLE } from '../modules/local/mageck/flutemle' +include { MAGECK_FLUTEMLE as MAGECK_FLUTEMLE_CONTRASTS } from '../modules/local/mageck/flutemle' +include { MAGECK_FLUTEMLE as MAGECK_FLUTEMLE_DAY0 } from '../modules/local/mageck/flutemle' include { VENNDIAGRAM } from '../modules/local/venndiagram' // nf-core modules include { FASTQC } from '../modules/nf-core/fastqc/main' @@ -277,8 +279,8 @@ workflow CRISPRSEQ_SCREENING { ch_mle = MATRICESCREATION.out.design_matrix.combine(ch_counts) MAGECK_MLE (ch_mle) ch_versions = ch_versions.mix(MAGECK_MLE.out.versions) - MAGECK_FLUTEMLE(MAGECK_MLE.out.gene_summary) - ch_versions = ch_versions.mix(MAGECK_FLUTEMLE.out.versions) + MAGECK_FLUTEMLE_CONTRASTS(MAGECK_MLE.out.gene_summary) + ch_versions = ch_versions.mix(MAGECK_FLUTEMLE_CONTRASTS.out.versions) ch_venndiagram = BAGEL2_PR.out.pr.join(MAGECK_MLE.out.gene_summary) VENNDIAGRAM(ch_venndiagram) ch_versions = ch_versions.mix(VENNDIAGRAM.out.versions) @@ -287,8 +289,8 @@ workflow CRISPRSEQ_SCREENING { ch_mle = Channel.of([id: "day0"]).merge(Channel.of([[]])).merge(ch_counts) MAGECK_MLE_DAY0 (ch_mle) ch_versions = ch_versions.mix(MAGECK_MLE_DAY0.out.versions) - MAGECK_FLUTEMLE(MAGECK_MLE_DAY0.out.gene_summary) - ch_versions = ch_versions.mix(MAGECK_FLUTEMLE.out.versions) + MAGECK_FLUTEMLE_DAY0(MAGECK_MLE_DAY0.out.gene_summary) + ch_versions = ch_versions.mix(MAGECK_FLUTEMLE_DAY0.out.versions) } } From b2844dc41518fdf30c9e38b003d973d71fb10cf1 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 16:15:13 +0200 Subject: [PATCH 166/226] revert back download_pipeline to fix linting issue --- .github/workflows/download_pipeline.yml | 22 +++++----------------- 1 file changed, 5 insertions(+), 17 deletions(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 6dad1232..08622fd5 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -28,14 +28,11 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v2 + uses: nf-core/setup-nextflow@v1 - - name: Disk space cleanup - uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 - - - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: - python-version: "3.12" + python-version: "3.11" architecture: "x64" - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: @@ -67,18 +64,9 @@ jobs: - name: Inspect download run: tree ./${{ env.REPOTITLE_LOWERCASE }} - - name: Run the downloaded pipeline (stub) - id: stub_run_pipeline - continue-on-error: true - env: - NXF_SINGULARITY_CACHEDIR: ./ - NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results - - name: Run the downloaded pipeline (stub run not supported) - id: run_pipeline - if: ${{ job.steps.stub_run_pipeline.status == failure() }} + - name: Run the downloaded pipeline env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results{% endraw %} + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results From ba2b4c36c81710107d6d5e0bb8b4db7b7ccc7b68 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 18:08:14 +0200 Subject: [PATCH 167/226] Fix linting --- .github/workflows/linting.yml | 65 ++++++++++++++++++++++++++++------- 1 file changed, 52 insertions(+), 13 deletions(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 073e1876..b8bdd214 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,33 +11,72 @@ on: types: [published] jobs: - pre-commit: + EditorConfig: runs-on: ubuntu-latest steps: - - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + - uses: actions/checkout@v3 - - name: Set up Python 3.11 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-node@v3 + + - name: Install editorconfig-checker + run: npm install -g editorconfig-checker + + - name: Run ECLint check + run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') + + Prettier: + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v3 + + - uses: actions/setup-node@v3 + + - name: Install Prettier + run: npm install -g prettier + + - name: Run Prettier --check + run: prettier --check ${GITHUB_WORKSPACE} + + PythonBlack: + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v3 + + - name: Check code lints with Black + uses: psf/black@stable + + # If the above check failed, post a comment on the PR explaining the failure + - name: Post PR comment + if: failure() + uses: mshick/add-pr-comment@v1 with: - python-version: 3.11 - cache: "pip" + message: | + ## Python linting (`black`) is failing + + To keep the code consistent with lots of contributors, we run automated code consistency checks. + To fix this CI test, please run: + + * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` + * Fix formatting errors in your pipeline: `black .` + + Once you push these changes the test should pass, and you can hide this comment :+1: - - name: Install pre-commit - run: pip install pre-commit + We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! - - name: Run pre-commit - run: pre-commit run --all-files + Thanks again for your contribution! + repo-token: ${{ secrets.GITHUB_TOKEN }} + allow-repeats: false nf-core: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + uses: actions/checkout@v3 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@v4 with: python-version: "3.11" architecture: "x64" @@ -60,7 +99,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + uses: actions/upload-artifact@v3 with: name: linting-logs path: | From df969dc5d0bdd56dac057eb62b47e50afb6e81f7 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Fri, 24 May 2024 18:10:21 +0200 Subject: [PATCH 168/226] Fixl inting --- .github/workflows/linting.yml | 65 +++++++---------------------------- 1 file changed, 13 insertions(+), 52 deletions(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index b8bdd214..073e1876 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,72 +11,33 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - - uses: actions/setup-node@v3 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v3 - - - uses: actions/setup-node@v3 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v3 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.11 + uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: 3.11 + cache: "pip" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" @@ -99,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: linting-logs path: | From e1afeb118c3780b2dbe377eedfa614ff613d38d4 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Mon, 27 May 2024 10:55:47 +0200 Subject: [PATCH 169/226] Delete useless file --- workflows/crisprseq.nf | 76 ------------------------------------------ 1 file changed, 76 deletions(-) delete mode 100644 workflows/crisprseq.nf diff --git a/workflows/crisprseq.nf b/workflows/crisprseq.nf deleted file mode 100644 index 467da216..00000000 --- a/workflows/crisprseq.nf +++ /dev/null @@ -1,76 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { paramsSummaryMap } from 'plugin/nf-validation' -include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' -include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' -include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_crisprseq_pipeline' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN MAIN WORKFLOW -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -workflow CRISPRSEQ { - - take: - ch_samplesheet // channel: samplesheet read in from --input - - main: - - ch_versions = Channel.empty() - ch_multiqc_files = Channel.empty() - - // - // MODULE: Run FastQC - // - FASTQC ( - ch_samplesheet - ) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - - // - // Collate and save software versions - // - softwareVersionsToYAML(ch_versions) - .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) - .set { ch_collated_versions } - - // - // MODULE: MultiQC - // - ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) - ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() - ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() - summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") - ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) - ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) - - MULTIQC ( - ch_multiqc_files.collect(), - ch_multiqc_config.toList(), - ch_multiqc_custom_config.toList(), - ch_multiqc_logo.toList() - ) - - emit: - multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html - versions = ch_versions // channel: [ path(versions.yml) ] -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - THE END -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ From 29810cbaa3092033da9a87eee7fc6f803c68dff1 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 11:00:11 +0200 Subject: [PATCH 170/226] Update nextflow.config MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Júlia Mir Pedrol --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 4a77bd55..60857ac1 100644 --- a/nextflow.config +++ b/nextflow.config @@ -140,7 +140,7 @@ profiles { shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false - docker.runOptions = '-u $(id -u):$(id -g)' + process.containerOptions = '-u $(id -u):$(id -g)' } arm { docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' From 27531c535190e9146f56affc544fcde53a487aef Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 11:00:30 +0200 Subject: [PATCH 171/226] Update nextflow.config MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Júlia Mir Pedrol --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 60857ac1..f936e3b4 100644 --- a/nextflow.config +++ b/nextflow.config @@ -64,7 +64,7 @@ params { hook_url = null help = false version = false - pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' + pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/' // Config options config_profile_name = null From d5eb5e1c1e8f43236d021dbfdc44a424beb241f3 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Mon, 27 May 2024 11:05:03 +0200 Subject: [PATCH 172/226] Fix duplicates --- modules.json | 159 ++++++++++----------------------------------------- 1 file changed, 30 insertions(+), 129 deletions(-) diff --git a/modules.json b/modules.json index 24afbeba..2fe895af 100644 --- a/modules.json +++ b/modules.json @@ -17,7 +17,7 @@ }, "bwa/index": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "6278bf9afd4a4b2d00fa6052250e73da3d91546f", "installed_by": ["modules"] }, "bwa/mem": { @@ -27,160 +27,48 @@ }, "cat/fastq": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "02fd5bd7275abad27aad32d5c852e0a9b1b98882", "installed_by": ["modules"] }, "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" + "installed_by": ["modules"] }, "cutadapt": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/cutadapt/cutadapt.diff" + "git_sha": "07881e42938b4f0070e864b45d424b01745bc3a4", + "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", + "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", "installed_by": ["modules"] }, "mageck/count": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/count/mageck-count.diff" - }, - "mageck/mle": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" - }, - "mageck/test": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/test/mageck-test.diff" - }, - "medaka": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/medaka/medaka.diff" - }, - "minimap2/align": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" - }, - "minimap2/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "mageck/count": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/count/mageck-count.diff" - }, "mageck/mle": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" - }, - "mageck/test": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/test/mageck-test.diff" - }, - "medaka": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/medaka/medaka.diff" - }, - "minimap2/align": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" - }, - "minimap2/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "mageck/count": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/count/mageck-count.diff" - }, - "mageck/mle": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" - }, "mageck/test": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/test/mageck-test.diff" - }, - "medaka": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/medaka/medaka.diff" - }, - "minimap2/align": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" - }, - "minimap2/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "mageck/count": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/count/mageck-count.diff" - }, - "mageck/mle": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" - }, - "mageck/test": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/test/mageck-test.diff" - }, "medaka": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/medaka/medaka.diff" + "installed_by": ["modules"] }, "minimap2/align": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" + "git_sha": "1a5a9e7b4009dcf34e6867dd1a5a1d9a718b027b", + "installed_by": ["modules"] }, "minimap2/index": { "branch": "master", @@ -199,7 +87,7 @@ }, "racon": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "f5ed3ac0834b68e80a00a06a61d04ce8e896f275", "installed_by": ["modules"] }, "samtools/index": { @@ -214,21 +102,34 @@ }, "vsearch/cluster": { "branch": "master", - "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", - "installed_by": ["subworkflows"] - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" + "git_sha": "d97b335eb448073c1b680710303c02a55f40c77c", + "installed_by": ["modules"] }, "vsearch/sort": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "97cf4c4a37a8931e19ef728de92f930a366669f2", "installed_by": ["modules"] } } }, "subworkflows": { - "nf-core": {} + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + } + } } } } From 107a310ad72ab3bf1784f2b598e459c67cb5098a Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Mon, 27 May 2024 11:08:09 +0200 Subject: [PATCH 173/226] Changes on other awsfull test too --- .github/workflows/awsfulltest_screening.yml | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/.github/workflows/awsfulltest_screening.yml b/.github/workflows/awsfulltest_screening.yml index 8c91e9cf..3e1ecf90 100644 --- a/.github/workflows/awsfulltest_screening.yml +++ b/.github/workflows/awsfulltest_screening.yml @@ -8,12 +8,12 @@ on: types: [published] workflow_dispatch: jobs: - run-tower: + run-platform: name: Run AWS full tests if: github.repository == 'nf-core/crisprseq' runs-on: ubuntu-latest steps: - - name: Launch workflow via tower + - name: Launch workflow via Seqera Platform uses: seqeralabs/action-tower-launch@v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} @@ -31,5 +31,5 @@ jobs: with: name: Tower debug log file path: | - tower_action_*.log - tower_action_*.json + seqera_platform_action_*.log + seqera_platform_action_*.json From dfdb224f78255acdeeecf82b11affb75ea730d35 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Mon, 27 May 2024 11:12:10 +0200 Subject: [PATCH 174/226] Put containerOptions again --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index f936e3b4..46a7701b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -143,7 +143,7 @@ profiles { process.containerOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + process.containerOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { singularity.enabled = true From b77c9b0cd5b996e7f574d9f33e74ef72e57b0201 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Mon, 27 May 2024 11:27:31 +0200 Subject: [PATCH 175/226] Add patches for modules.json --- modules.json | 21 ++++++++++++++------- 1 file changed, 14 insertions(+), 7 deletions(-) diff --git a/modules.json b/modules.json index 2fe895af..a65d57d9 100644 --- a/modules.json +++ b/modules.json @@ -33,12 +33,14 @@ "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" }, "cutadapt": { "branch": "master", "git_sha": "07881e42938b4f0070e864b45d424b01745bc3a4", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/cutadapt/cutadapt.diff" }, "fastqc": { "branch": "master", @@ -48,27 +50,32 @@ "mageck/count": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/count/mageck-count.diff" }, "mageck/mle": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" }, "mageck/test": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/test/mageck-test.diff" }, "medaka": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/medaka/medaka.diff" }, "minimap2/align": { "branch": "master", "git_sha": "1a5a9e7b4009dcf34e6867dd1a5a1d9a718b027b", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" }, "minimap2/index": { "branch": "master", From c2b7185e7de7f766cac30e4e8e5de518d10947ac Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Mon, 27 May 2024 09:41:08 +0000 Subject: [PATCH 176/226] Revert "Add patches for modules.json" This reverts commit b77c9b0cd5b996e7f574d9f33e74ef72e57b0201. --- modules.json | 21 +++++++-------------- 1 file changed, 7 insertions(+), 14 deletions(-) diff --git a/modules.json b/modules.json index a65d57d9..2fe895af 100644 --- a/modules.json +++ b/modules.json @@ -33,14 +33,12 @@ "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" + "installed_by": ["modules"] }, "cutadapt": { "branch": "master", "git_sha": "07881e42938b4f0070e864b45d424b01745bc3a4", - "installed_by": ["modules"], - "patch": "modules/nf-core/cutadapt/cutadapt.diff" + "installed_by": ["modules"] }, "fastqc": { "branch": "master", @@ -50,32 +48,27 @@ "mageck/count": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/count/mageck-count.diff" + "installed_by": ["modules"] }, "mageck/mle": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" + "installed_by": ["modules"] }, "mageck/test": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/test/mageck-test.diff" + "installed_by": ["modules"] }, "medaka": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/medaka/medaka.diff" + "installed_by": ["modules"] }, "minimap2/align": { "branch": "master", "git_sha": "1a5a9e7b4009dcf34e6867dd1a5a1d9a718b027b", - "installed_by": ["modules"], - "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" + "installed_by": ["modules"] }, "minimap2/index": { "branch": "master", From 6f44a1262534db531270df068d95e061a5b1756d Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Mon, 27 May 2024 09:41:49 +0000 Subject: [PATCH 177/226] Revert "Fix duplicates" This reverts commit d5eb5e1c1e8f43236d021dbfdc44a424beb241f3. --- modules.json | 159 +++++++++++++++++++++++++++++++++++++++++---------- 1 file changed, 129 insertions(+), 30 deletions(-) diff --git a/modules.json b/modules.json index 2fe895af..24afbeba 100644 --- a/modules.json +++ b/modules.json @@ -17,7 +17,7 @@ }, "bwa/index": { "branch": "master", - "git_sha": "6278bf9afd4a4b2d00fa6052250e73da3d91546f", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "bwa/mem": { @@ -27,49 +27,161 @@ }, "cat/fastq": { "branch": "master", - "git_sha": "02fd5bd7275abad27aad32d5c852e0a9b1b98882", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" }, "cutadapt": { "branch": "master", - "git_sha": "07881e42938b4f0070e864b45d424b01745bc3a4", - "installed_by": ["modules"] + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/cutadapt/cutadapt.diff" }, "fastqc": { "branch": "master", - "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["modules"] }, "mageck/count": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/count/mageck-count.diff" }, "mageck/mle": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" }, "mageck/test": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/test/mageck-test.diff" + }, + "medaka": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/medaka/medaka.diff" + }, + "minimap2/align": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" + }, + "minimap2/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, + "mageck/count": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/count/mageck-count.diff" + }, + "mageck/mle": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" + }, + "mageck/test": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/test/mageck-test.diff" + }, "medaka": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/medaka/medaka.diff" + }, + "minimap2/align": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" + }, + "minimap2/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, + "mageck/count": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/count/mageck-count.diff" + }, + "mageck/mle": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" + }, + "mageck/test": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/test/mageck-test.diff" + }, + "medaka": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/medaka/medaka.diff" + }, "minimap2/align": { "branch": "master", - "git_sha": "1a5a9e7b4009dcf34e6867dd1a5a1d9a718b027b", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" + }, + "minimap2/index": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, + "mageck/count": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/count/mageck-count.diff" + }, + "mageck/mle": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" + }, + "mageck/test": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/mageck/test/mageck-test.diff" + }, + "medaka": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/medaka/medaka.diff" + }, + "minimap2/align": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" + }, "minimap2/index": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", @@ -87,7 +199,7 @@ }, "racon": { "branch": "master", - "git_sha": "f5ed3ac0834b68e80a00a06a61d04ce8e896f275", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "samtools/index": { @@ -102,34 +214,21 @@ }, "vsearch/cluster": { "branch": "master", - "git_sha": "d97b335eb448073c1b680710303c02a55f40c77c", - "installed_by": ["modules"] + "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", + "installed_by": ["subworkflows"] + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"], + "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" }, "vsearch/sort": { "branch": "master", - "git_sha": "97cf4c4a37a8931e19ef728de92f930a366669f2", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] } } }, "subworkflows": { - "nf-core": { - "utils_nextflow_pipeline": { - "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] - }, - "utils_nfcore_pipeline": { - "branch": "master", - "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", - "installed_by": ["subworkflows"] - }, - "utils_nfvalidation_plugin": { - "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] - } - } + "nf-core": {} } } } From 6525a4f11b9ca3d0b95d83c8f13c199f0cd6747c Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Mon, 27 May 2024 10:02:55 +0000 Subject: [PATCH 178/226] remove changes from modules.json --- modules.json | 107 --------------------------------------------------- 1 file changed, 107 deletions(-) diff --git a/modules.json b/modules.json index 24afbeba..3846e879 100644 --- a/modules.json +++ b/modules.json @@ -82,111 +82,6 @@ "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "mageck/count": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/count/mageck-count.diff" - }, - "mageck/mle": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" - }, - "mageck/test": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/test/mageck-test.diff" - }, - "medaka": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/medaka/medaka.diff" - }, - "minimap2/align": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" - }, - "minimap2/index": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, - "mageck/count": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/count/mageck-count.diff" - }, - "mageck/mle": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" - }, - "mageck/test": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/test/mageck-test.diff" - }, - "medaka": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/medaka/medaka.diff" - }, - "minimap2/align": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" - }, - "minimap2/index": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, - "mageck/count": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/count/mageck-count.diff" - }, - "mageck/mle": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" - }, - "mageck/test": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/mageck/test/mageck-test.diff" - }, - "medaka": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/medaka/medaka.diff" - }, - "minimap2/align": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], - "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" - }, - "minimap2/index": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", @@ -214,8 +109,6 @@ }, "vsearch/cluster": { "branch": "master", - "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", - "installed_by": ["subworkflows"] "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"], "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" From e9d21a3b9d71fd22788582fed648117008c1a8b2 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Mon, 27 May 2024 10:12:31 +0000 Subject: [PATCH 179/226] update all modules --- modules.json | 28 +- modules/nf-core/bowtie2/align/environment.yml | 4 +- modules/nf-core/bowtie2/align/main.nf | 41 +- .../bowtie2/align/tests/cram_crai.config | 5 + .../bowtie2/align/tests/large_index.config | 5 + .../nf-core/bowtie2/align/tests/main.nf.test | 623 ++++++++++++++++++ .../bowtie2/align/tests/main.nf.test.snap | 311 +++++++++ .../nf-core/bowtie2/align/tests/sam.config | 5 + .../nf-core/bowtie2/align/tests/sam2.config | 5 + modules/nf-core/bowtie2/align/tests/tags.yml | 2 + modules/nf-core/bowtie2/build/environment.yml | 2 +- modules/nf-core/bowtie2/build/main.nf | 4 +- .../nf-core/bowtie2/build/tests/main.nf.test | 31 + .../bowtie2/build/tests/main.nf.test.snap | 45 ++ modules/nf-core/bowtie2/build/tests/tags.yml | 2 + modules/nf-core/bwa/index/environment.yml | 2 +- modules/nf-core/bwa/index/main.nf | 4 +- modules/nf-core/bwa/index/meta.yml | 1 + modules/nf-core/bwa/index/tests/main.nf.test | 4 +- .../nf-core/bwa/index/tests/main.nf.test.snap | 10 +- modules/nf-core/bwa/mem/environment.yml | 5 +- modules/nf-core/bwa/mem/main.nf | 35 +- modules/nf-core/bwa/mem/meta.yml | 18 + modules/nf-core/bwa/mem/tests/main.nf.test | 205 +++++- .../nf-core/bwa/mem/tests/main.nf.test.snap | 230 ++++--- modules/nf-core/cat/fastq/environment.yml | 2 +- modules/nf-core/cat/fastq/main.nf | 1 - modules/nf-core/cat/fastq/tests/main.nf.test | 65 +- .../nf-core/cat/fastq/tests/main.nf.test.snap | 185 ++++-- modules/nf-core/cutadapt/cutadapt.diff | 6 +- modules/nf-core/cutadapt/environment.yml | 2 +- modules/nf-core/cutadapt/main.nf | 7 +- modules/nf-core/cutadapt/tests/main.nf.test | 69 ++ .../nf-core/cutadapt/tests/main.nf.test.snap | 46 ++ .../nf-core/cutadapt/tests/nextflow.config | 7 + modules/nf-core/cutadapt/tests/tags.yml | 2 + .../nf-core/minimap2/align/environment.yml | 5 +- modules/nf-core/minimap2/align/main.nf | 29 +- .../minimap2/align/minimap2-align.diff | 26 +- .../nf-core/minimap2/align/tests/main.nf.test | 181 +++++ .../minimap2/align/tests/main.nf.test.snap | 69 ++ modules/nf-core/minimap2/align/tests/tags.yml | 2 + .../nf-core/minimap2/index/environment.yml | 2 +- modules/nf-core/minimap2/index/main.nf | 16 +- .../nf-core/minimap2/index/tests/main.nf.test | 32 + .../minimap2/index/tests/main.nf.test.snap | 68 ++ modules/nf-core/minimap2/index/tests/tags.yml | 2 + modules/nf-core/racon/tests/main.nf.test | 34 + modules/nf-core/racon/tests/main.nf.test.snap | 37 ++ modules/nf-core/racon/tests/tags.yml | 2 + .../nf-core/samtools/index/environment.yml | 3 +- modules/nf-core/samtools/index/main.nf | 4 +- .../nf-core/samtools/index/tests/main.nf.test | 36 +- .../samtools/index/tests/main.nf.test.snap | 54 +- modules/nf-core/seqtk/seq/main.nf | 16 + modules/nf-core/seqtk/seq/tests/main.nf.test | 88 +++ .../nf-core/seqtk/seq/tests/main.nf.test.snap | 101 +++ .../nf-core/seqtk/seq/tests/standard.config | 5 + modules/nf-core/seqtk/seq/tests/tags.yml | 2 + .../vsearch/cluster/tests/main.nf.test | 132 ++++ .../vsearch/cluster/tests/main.nf.test.snap | 487 ++++++++++++++ .../cluster/tests/nextflow_fast.config | 7 + .../cluster/tests/nextflow_size.config | 7 + .../cluster/tests/nextflow_smallmem.config | 7 + .../cluster/tests/nextflow_unoise.config | 7 + .../cluster/tests/nextflow_userout.config | 7 + .../nf-core/vsearch/cluster/tests/tags.yml | 2 + .../nf-core/vsearch/sort/tests/main.nf.test | 60 ++ .../vsearch/sort/tests/main.nf.test.snap | 72 ++ .../vsearch/sort/tests/nextflow.config | 6 + modules/nf-core/vsearch/sort/tests/tags.yml | 2 + 71 files changed, 3317 insertions(+), 312 deletions(-) create mode 100644 modules/nf-core/bowtie2/align/tests/cram_crai.config create mode 100644 modules/nf-core/bowtie2/align/tests/large_index.config create mode 100644 modules/nf-core/bowtie2/align/tests/main.nf.test create mode 100644 modules/nf-core/bowtie2/align/tests/main.nf.test.snap create mode 100644 modules/nf-core/bowtie2/align/tests/sam.config create mode 100644 modules/nf-core/bowtie2/align/tests/sam2.config create mode 100644 modules/nf-core/bowtie2/align/tests/tags.yml create mode 100644 modules/nf-core/bowtie2/build/tests/main.nf.test create mode 100644 modules/nf-core/bowtie2/build/tests/main.nf.test.snap create mode 100644 modules/nf-core/bowtie2/build/tests/tags.yml create mode 100644 modules/nf-core/cutadapt/tests/main.nf.test create mode 100644 modules/nf-core/cutadapt/tests/main.nf.test.snap create mode 100644 modules/nf-core/cutadapt/tests/nextflow.config create mode 100644 modules/nf-core/cutadapt/tests/tags.yml create mode 100644 modules/nf-core/minimap2/align/tests/main.nf.test create mode 100644 modules/nf-core/minimap2/align/tests/main.nf.test.snap create mode 100644 modules/nf-core/minimap2/align/tests/tags.yml create mode 100644 modules/nf-core/minimap2/index/tests/main.nf.test create mode 100644 modules/nf-core/minimap2/index/tests/main.nf.test.snap create mode 100644 modules/nf-core/minimap2/index/tests/tags.yml create mode 100644 modules/nf-core/racon/tests/main.nf.test create mode 100644 modules/nf-core/racon/tests/main.nf.test.snap create mode 100644 modules/nf-core/racon/tests/tags.yml create mode 100644 modules/nf-core/seqtk/seq/tests/main.nf.test create mode 100644 modules/nf-core/seqtk/seq/tests/main.nf.test.snap create mode 100644 modules/nf-core/seqtk/seq/tests/standard.config create mode 100644 modules/nf-core/seqtk/seq/tests/tags.yml create mode 100644 modules/nf-core/vsearch/cluster/tests/main.nf.test create mode 100644 modules/nf-core/vsearch/cluster/tests/main.nf.test.snap create mode 100644 modules/nf-core/vsearch/cluster/tests/nextflow_fast.config create mode 100644 modules/nf-core/vsearch/cluster/tests/nextflow_size.config create mode 100644 modules/nf-core/vsearch/cluster/tests/nextflow_smallmem.config create mode 100644 modules/nf-core/vsearch/cluster/tests/nextflow_unoise.config create mode 100644 modules/nf-core/vsearch/cluster/tests/nextflow_userout.config create mode 100644 modules/nf-core/vsearch/cluster/tests/tags.yml create mode 100644 modules/nf-core/vsearch/sort/tests/main.nf.test create mode 100644 modules/nf-core/vsearch/sort/tests/main.nf.test.snap create mode 100644 modules/nf-core/vsearch/sort/tests/nextflow.config create mode 100644 modules/nf-core/vsearch/sort/tests/tags.yml diff --git a/modules.json b/modules.json index 3846e879..3e576767 100644 --- a/modules.json +++ b/modules.json @@ -7,27 +7,27 @@ "nf-core": { "bowtie2/align": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "0fe30831abbc2ed115e46e92330edf38f56edc3d", "installed_by": ["modules"] }, "bowtie2/build": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "1fea64f5132a813ec97c1c6d3a74e0aee7142b6d", "installed_by": ["modules"] }, "bwa/index": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "086fa66260595e123b0ea47a6512539b72a9afa3", "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "0c34b8159f62cde451c4ff249629c9d0a4f3f9c3", "installed_by": ["modules"] }, "cat/fastq": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "4fc983ad0b30e6e32696fa7d980c76c7bfe1c03e", "installed_by": ["modules"] }, "crisprcleanr/normalize": { @@ -38,13 +38,13 @@ }, "cutadapt": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "6618151ed69274863dc6fe6d2920afa90abaca1f", "installed_by": ["modules"], "patch": "modules/nf-core/cutadapt/cutadapt.diff" }, "fastqc": { "branch": "master", - "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", + "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", "installed_by": ["modules"] }, "mageck/count": { @@ -73,13 +73,13 @@ }, "minimap2/align": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", "installed_by": ["modules"], "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" }, "minimap2/index": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", "installed_by": ["modules"] }, "multiqc": { @@ -94,28 +94,28 @@ }, "racon": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "f5ed3ac0834b68e80a00a06a61d04ce8e896f275", "installed_by": ["modules"] }, "samtools/index": { "branch": "master", - "git_sha": "5394565c5fe4c760e5b35977ec7607c62e81d1f8", + "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", "installed_by": ["modules"] }, "seqtk/seq": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "7f88aae93c69586c0789322b77743ee0ef469502", "installed_by": ["modules"] }, "vsearch/cluster": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "d97b335eb448073c1b680710303c02a55f40c77c", "installed_by": ["modules"], "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" }, "vsearch/sort": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "97cf4c4a37a8931e19ef728de92f930a366669f2", "installed_by": ["modules"] } } diff --git a/modules/nf-core/bowtie2/align/environment.yml b/modules/nf-core/bowtie2/align/environment.yml index afc3ea87..d2796359 100644 --- a/modules/nf-core/bowtie2/align/environment.yml +++ b/modules/nf-core/bowtie2/align/environment.yml @@ -4,6 +4,6 @@ channels: - bioconda - defaults dependencies: - - bioconda::bowtie2=2.4.4 - - bioconda::samtools=1.16.1 + - bioconda::bowtie2=2.5.2 + - bioconda::samtools=1.18 - conda-forge::pigz=2.6 diff --git a/modules/nf-core/bowtie2/align/main.nf b/modules/nf-core/bowtie2/align/main.nf index e67f6a06..96a7027d 100644 --- a/modules/nf-core/bowtie2/align/main.nf +++ b/modules/nf-core/bowtie2/align/main.nf @@ -4,19 +4,24 @@ process BOWTIE2_ALIGN { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' : - 'biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' }" + 'https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:f70b31a2db15c023d641c32f433fb02cd04df5a6-0' : + 'biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:f70b31a2db15c023d641c32f433fb02cd04df5a6-0' }" input: tuple val(meta) , path(reads) tuple val(meta2), path(index) + tuple val(meta3), path(fasta) val save_unaligned val sort_bam output: - tuple val(meta), path("*.{bam,sam}"), emit: aligned + tuple val(meta), path("*.sam") , emit: sam , optional:true + tuple val(meta), path("*.bam") , emit: bam , optional:true + tuple val(meta), path("*.cram") , emit: cram , optional:true + tuple val(meta), path("*.csi") , emit: csi , optional:true + tuple val(meta), path("*.crai") , emit: crai , optional:true tuple val(meta), path("*.log") , emit: log - tuple val(meta), path("*fastq.gz") , emit: fastq, optional:true + tuple val(meta), path("*fastq.gz") , emit: fastq , optional:true path "versions.yml" , emit: versions when: @@ -39,7 +44,10 @@ process BOWTIE2_ALIGN { def samtools_command = sort_bam ? 'sort' : 'view' def extension_pattern = /(--output-fmt|-O)+\s+(\S+)/ - def extension = (args2 ==~ extension_pattern) ? (args2 =~ extension_pattern)[0][2].toLowerCase() : "bam" + def extension_matcher = (args2 =~ extension_pattern) + def extension = extension_matcher.getCount() > 0 ? extension_matcher[0][2].toLowerCase() : "bam" + def reference = fasta && extension=="cram" ? "--reference ${fasta}" : "" + if (!fasta && extension=="cram") error "Fasta reference is required for CRAM output" """ INDEX=`find -L ./ -name "*.rev.1.bt2" | sed "s/\\.rev.1.bt2\$//"` @@ -52,8 +60,8 @@ process BOWTIE2_ALIGN { --threads $task.cpus \\ $unaligned \\ $args \\ - 2> ${prefix}.bowtie2.log \\ - | samtools $samtools_command $args2 --threads $task.cpus -o ${prefix}.${extension} - + 2> >(tee ${prefix}.bowtie2.log >&2) \\ + | samtools $samtools_command $args2 --threads $task.cpus ${reference} -o ${prefix}.${extension} - if [ -f ${prefix}.unmapped.fastq.1.gz ]; then mv ${prefix}.unmapped.fastq.1.gz ${prefix}.unmapped_1.fastq.gz @@ -76,12 +84,27 @@ process BOWTIE2_ALIGN { def prefix = task.ext.prefix ?: "${meta.id}" def extension_pattern = /(--output-fmt|-O)+\s+(\S+)/ def extension = (args2 ==~ extension_pattern) ? (args2 =~ extension_pattern)[0][2].toLowerCase() : "bam" + def create_unmapped = "" + if (meta.single_end) { + create_unmapped = save_unaligned ? "touch ${prefix}.unmapped.fastq.gz" : "" + } else { + create_unmapped = save_unaligned ? "touch ${prefix}.unmapped_1.fastq.gz && touch ${prefix}.unmapped_2.fastq.gz" : "" + } + def reference = fasta && extension=="cram" ? "--reference ${fasta}" : "" + if (!fasta && extension=="cram") error "Fasta reference is required for CRAM output" + + def create_index = "" + if (extension == "cram") { + create_index = "touch ${prefix}.crai" + } else if (extension == "bam") { + create_index = "touch ${prefix}.csi" + } """ touch ${prefix}.${extension} + ${create_index} touch ${prefix}.bowtie2.log - touch ${prefix}.unmapped_1.fastq.gz - touch ${prefix}.unmapped_2.fastq.gz + ${create_unmapped} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/bowtie2/align/tests/cram_crai.config b/modules/nf-core/bowtie2/align/tests/cram_crai.config new file mode 100644 index 00000000..03f1d5e5 --- /dev/null +++ b/modules/nf-core/bowtie2/align/tests/cram_crai.config @@ -0,0 +1,5 @@ +process { + withName: BOWTIE2_ALIGN { + ext.args2 = '--output-fmt cram --write-index' + } +} diff --git a/modules/nf-core/bowtie2/align/tests/large_index.config b/modules/nf-core/bowtie2/align/tests/large_index.config new file mode 100644 index 00000000..fdc1c59d --- /dev/null +++ b/modules/nf-core/bowtie2/align/tests/large_index.config @@ -0,0 +1,5 @@ +process { + withName: BOWTIE2_BUILD { + ext.args = '--large-index' + } +} \ No newline at end of file diff --git a/modules/nf-core/bowtie2/align/tests/main.nf.test b/modules/nf-core/bowtie2/align/tests/main.nf.test new file mode 100644 index 00000000..03aeaf9e --- /dev/null +++ b/modules/nf-core/bowtie2/align/tests/main.nf.test @@ -0,0 +1,623 @@ +nextflow_process { + + name "Test Process BOWTIE2_ALIGN" + script "../main.nf" + process "BOWTIE2_ALIGN" + tag "modules" + tag "modules_nfcore" + tag "bowtie2" + tag "bowtie2/build" + tag "bowtie2/align" + + test("sarscov2 - fastq, index, fasta, false, false - bam") { + + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = [[ id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)] + input[3] = false //save_unaligned + input[4] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, index, fasta, false, false - sam") { + + config "./sam.config" + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = [[ id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)] + input[3] = false //save_unaligned + input[4] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.sam[0][1]).readLines()[0..4], + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, index, fasta, false, false - sam2") { + + config "./sam2.config" + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = [[ id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)] + input[3] = false //save_unaligned + input[4] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.sam[0][1]).readLines()[0..4], + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, index, fasta, false, true - bam") { + + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = [[ id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)] + input[3] = false //save_unaligned + input[4] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, fasta, false, false - bam") { + + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = [[ id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)] + input[3] = false //save_unaligned + input[4] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, fasta, false, true - bam") { + + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = [[ id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)] + input[3] = false //save_unaligned + input[4] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, large_index, fasta, false, false - bam") { + + config "./large_index.config" + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = [[ id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)] + input[3] = false //save_unaligned + input[4] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], large_index, fasta, false, false - bam") { + + config "./large_index.config" + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = [[ id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)] + input[3] = false //save_unaligned + input[4] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, fasta, true, false - bam") { + + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = [[ id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)] + input[3] = false //save_unaligned + input[4] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, index, fasta, true, false - bam") { + + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = [[ id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)] + input[3] = false //save_unaligned + input[4] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, fasta, true, true - cram") { + + config "./cram_crai.config" + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = [[ id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)] + input[3] = false //save_unaligned + input[4] = true //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.cram[0][1]).name, + file(process.out.crai[0][1]).name + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, fasta, false, false - stub") { + + options "-stub" + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = [[ id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)] + input[3] = false //save_unaligned + input[4] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + file(process.out.csi[0][1]).name, + file(process.out.log[0][1]).name, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, index, fasta, true, false - stub") { + + options "-stub" + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = [[ id:'test'], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)] + input[3] = false //save_unaligned + input[4] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + file(process.out.csi[0][1]).name, + file(process.out.log[0][1]).name, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/bowtie2/align/tests/main.nf.test.snap b/modules/nf-core/bowtie2/align/tests/main.nf.test.snap new file mode 100644 index 00000000..028e7da6 --- /dev/null +++ b/modules/nf-core/bowtie2/align/tests/main.nf.test.snap @@ -0,0 +1,311 @@ +{ + "sarscov2 - [fastq1, fastq2], large_index, fasta, false, false - bam": { + "content": [ + "test.bam", + [ + [ + { + "id": "test", + "single_end": false + }, + "test.bowtie2.log:md5,bd89ce1b28c93bf822bae391ffcedd19" + ] + ], + [ + + ], + [ + "versions.yml:md5,01d18ab035146ea790e9a0f70adb758f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T13:19:25.337323" + }, + "sarscov2 - fastq, index, fasta, false, false - sam2": { + "content": [ + [ + "ERR5069949.2151832\t16\tMT192765.1\t17453\t42\t150M\t*\t0\t0\tACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGA\tAAAA versions.yml "${task.process}": @@ -42,9 +53,19 @@ process BWA_MEM { """ stub: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + def samtools_command = sort_bam ? 'sort' : 'view' + def extension = args2.contains("--output-fmt sam") ? "sam" : + args2.contains("--output-fmt cram") ? "cram": + sort_bam && args2.contains("-O cram")? "cram": + !sort_bam && args2.contains("-C") ? "cram": + "bam" """ - touch ${prefix}.bam + touch ${prefix}.${extension} + touch ${prefix}.csi + touch ${prefix}.crai cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/bwa/mem/meta.yml b/modules/nf-core/bwa/mem/meta.yml index 440fb1f9..1532c261 100644 --- a/modules/nf-core/bwa/mem/meta.yml +++ b/modules/nf-core/bwa/mem/meta.yml @@ -37,6 +37,10 @@ input: type: file description: BWA genome index files pattern: "Directory containing BWA index *.{amb,ann,bwt,pac,sa}" + - fasta: + type: file + description: Reference genome in FASTA format + pattern: "*.{fasta,fa}" - sort_bam: type: boolean description: use samtools sort (true) or samtools view (false) @@ -46,6 +50,18 @@ output: type: file description: Output BAM file containing read alignments pattern: "*.{bam}" + - cram: + type: file + description: Output CRAM file containing read alignments + pattern: "*.{cram}" + - csi: + type: file + description: Optional index file for BAM file + pattern: "*.{csi}" + - crai: + type: file + description: Optional index file for CRAM file + pattern: "*.{crai}" - versions: type: file description: File containing software versions @@ -53,6 +69,8 @@ output: authors: - "@drpatelh" - "@jeremy1805" + - "@matthdsm" maintainers: - "@drpatelh" - "@jeremy1805" + - "@matthdsm" diff --git a/modules/nf-core/bwa/mem/tests/main.nf.test b/modules/nf-core/bwa/mem/tests/main.nf.test index b199bb70..463b76f8 100644 --- a/modules/nf-core/bwa/mem/tests/main.nf.test +++ b/modules/nf-core/bwa/mem/tests/main.nf.test @@ -5,6 +5,7 @@ nextflow_process { tag "modules" tag "bwa" tag "bwa/mem" + tag "bwa/index" script "../main.nf" process "BWA_MEM" @@ -17,7 +18,7 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } @@ -30,11 +31,12 @@ nextflow_process { input[0] = [ [ id:'test', single_end:true ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] ] input[1] = BWA_INDEX.out.index - input[2] = false + input[2] = [[id: 'test'],file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + input[3] = false """ } } @@ -42,7 +44,14 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.cram, + process.out.csi, + process.out.crai, + process.out.versions, + file(process.out.bam[0][1]).name + ).match() + } ) } @@ -57,7 +66,7 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } @@ -70,11 +79,12 @@ nextflow_process { input[0] = [ [ id:'test', single_end:true ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] ] input[1] = BWA_INDEX.out.index - input[2] = true + input[2] = [[id: 'test'],file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + input[3] = true """ } } @@ -82,7 +92,14 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.cram, + process.out.csi, + process.out.crai, + process.out.versions, + file(process.out.bam[0][1]).name + ).match() + } ) } @@ -97,7 +114,7 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } @@ -110,12 +127,13 @@ nextflow_process { input[0] = [ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] ] input[1] = BWA_INDEX.out.index - input[2] = false + input[2] = [[id: 'test'],file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + input[3] = false """ } } @@ -123,7 +141,14 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.cram, + process.out.csi, + process.out.crai, + process.out.versions, + file(process.out.bam[0][1]).name + ).match() + } ) } @@ -138,7 +163,56 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = [[id: 'test'],file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + input[3] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.cram, + process.out.csi, + process.out.crai, + process.out.versions, + file(process.out.bam[0][1]).name + ).match() + } + ) + } + + } + + test("Paired-End - no fasta") { + + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } @@ -151,12 +225,60 @@ nextflow_process { input[0] = [ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = [[:],[]] + input[3] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.cram, + process.out.csi, + process.out.crai, + process.out.versions, + file(process.out.bam[0][1]).name + ).match() + } + ) + } + + } + + test("Single-end - stub") { + options "-stub" + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + """ + } + } + } + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] ] input[1] = BWA_INDEX.out.index - input[2] = true + input[2] = [[id: 'test'],file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + input[3] = false """ } } @@ -164,9 +286,56 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + file(process.out.bam[0][1]).name, + file(process.out.csi[0][1]).name, + process.out.versions + ).match() } ) } + } + + test("Paired-end - stub") { + options "-stub" + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + """ + } + } + } + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = [[id: 'test'],file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + input[3] = false + """ + } + } + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + file(process.out.csi[0][1]).name, + process.out.versions + ).match() } + ) + } } } diff --git a/modules/nf-core/bwa/mem/tests/main.nf.test.snap b/modules/nf-core/bwa/mem/tests/main.nf.test.snap index ea3bfed4..038ee7b7 100644 --- a/modules/nf-core/bwa/mem/tests/main.nf.test.snap +++ b/modules/nf-core/bwa/mem/tests/main.nf.test.snap @@ -1,126 +1,140 @@ { "Single-End": { "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": true - }, - "test.bam:md5,3d43027d4163ada97911b814001511e5" - ] - ], - "1": [ - "versions.yml:md5,809f4a8c7f0c8497a9099dab9d6cc71e" - ], - "bam": [ - [ - { - "id": "test", - "single_end": true - }, - "test.bam:md5,3d43027d4163ada97911b814001511e5" - ] - ], - "versions": [ - "versions.yml:md5,809f4a8c7f0c8497a9099dab9d6cc71e" - ] - } + [ + + ], + [ + + ], + [ + + ], + [ + "versions.yml:md5,478b816fbd37871f5e8c617833d51d80" + ], + "test.bam" ], - "timestamp": "2023-10-18T11:02:55.420631681" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-20T08:44:32.953673185" }, "Single-End Sort": { "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": true - }, - "test.bam:md5,61eac1213d2bf5e88e225e545010e9b8" - ] - ], - "1": [ - "versions.yml:md5,809f4a8c7f0c8497a9099dab9d6cc71e" - ], - "bam": [ - [ - { - "id": "test", - "single_end": true - }, - "test.bam:md5,61eac1213d2bf5e88e225e545010e9b8" - ] - ], - "versions": [ - "versions.yml:md5,809f4a8c7f0c8497a9099dab9d6cc71e" - ] - } + [ + + ], + [ + + ], + [ + + ], + [ + "versions.yml:md5,478b816fbd37871f5e8c617833d51d80" + ], + "test.bam" ], - "timestamp": "2023-10-18T11:03:02.646869498" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-20T08:44:45.27066093" }, "Paired-End": { "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": false - }, - "test.bam:md5,809ccfe4300fa5005a9d0d4dc09b1a36" - ] - ], - "1": [ - "versions.yml:md5,809f4a8c7f0c8497a9099dab9d6cc71e" - ], - "bam": [ - [ - { - "id": "test", - "single_end": false - }, - "test.bam:md5,809ccfe4300fa5005a9d0d4dc09b1a36" - ] - ], - "versions": [ - "versions.yml:md5,809f4a8c7f0c8497a9099dab9d6cc71e" - ] - } + [ + + ], + [ + + ], + [ + + ], + [ + "versions.yml:md5,478b816fbd37871f5e8c617833d51d80" + ], + "test.bam" ], - "timestamp": "2023-10-18T11:03:09.793041294" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-20T08:44:57.706852274" }, "Paired-End Sort": { "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": false - }, - "test.bam:md5,2622f4380f992c505af7dab8c256313f" - ] - ], - "1": [ - "versions.yml:md5,809f4a8c7f0c8497a9099dab9d6cc71e" - ], - "bam": [ - [ - { - "id": "test", - "single_end": false - }, - "test.bam:md5,2622f4380f992c505af7dab8c256313f" - ] - ], - "versions": [ - "versions.yml:md5,809f4a8c7f0c8497a9099dab9d6cc71e" - ] - } + [ + + ], + [ + + ], + [ + + ], + [ + "versions.yml:md5,478b816fbd37871f5e8c617833d51d80" + ], + "test.bam" ], - "timestamp": "2023-10-18T11:04:43.662093286" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-20T08:45:10.376505036" + }, + "Single-end - stub": { + "content": [ + "test.bam", + "test.csi", + [ + "versions.yml:md5,478b816fbd37871f5e8c617833d51d80" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-20T08:46:07.182072398" + }, + "Paired-End - no fasta": { + "content": [ + [ + + ], + [ + + ], + [ + + ], + [ + "versions.yml:md5,478b816fbd37871f5e8c617833d51d80" + ], + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-20T08:45:53.813076501" + }, + "Paired-end - stub": { + "content": [ + "test.bam", + "test.csi", + [ + "versions.yml:md5,478b816fbd37871f5e8c617833d51d80" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-20T08:46:18.412916364" } } \ No newline at end of file diff --git a/modules/nf-core/cat/fastq/environment.yml b/modules/nf-core/cat/fastq/environment.yml index bff93add..8c69b121 100644 --- a/modules/nf-core/cat/fastq/environment.yml +++ b/modules/nf-core/cat/fastq/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - conda-forge::sed=4.7 + - conda-forge::coreutils=8.30 diff --git a/modules/nf-core/cat/fastq/main.nf b/modules/nf-core/cat/fastq/main.nf index 3d963784..f132b2ad 100644 --- a/modules/nf-core/cat/fastq/main.nf +++ b/modules/nf-core/cat/fastq/main.nf @@ -76,5 +76,4 @@ process CAT_FASTQ { """ } } - } diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test b/modules/nf-core/cat/fastq/tests/main.nf.test index f5f94182..a71dcb8d 100644 --- a/modules/nf-core/cat/fastq/tests/main.nf.test +++ b/modules/nf-core/cat/fastq/tests/main.nf.test @@ -1,3 +1,5 @@ +// NOTE The version snaps may not be consistant +// https://github.com/nf-core/modules/pull/4087#issuecomment-1767948035 nextflow_process { name "Test Process CAT_FASTQ" @@ -16,11 +18,11 @@ nextflow_process { } process { """ - input[0] = [ - [ id:'test', single_end:true ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true) ] - ] + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)] + ]) """ } } @@ -28,8 +30,7 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out.reads).match() }, - { assert path(process.out.versions.get(0)).getText().contains("cat") } + { assert snapshot(process.out).match() } ) } } @@ -42,13 +43,13 @@ nextflow_process { } process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true)] + ]) """ } } @@ -56,8 +57,7 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out.reads).match() }, - { assert path(process.out.versions.get(0)).getText().contains("cat") } + { assert snapshot(process.out).match() } ) } } @@ -70,11 +70,11 @@ nextflow_process { } process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:true ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)] + ]) """ } } @@ -82,8 +82,7 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out.reads).match() }, - { assert path(process.out.versions.get(0)).getText().contains("cat") } + { assert snapshot(process.out).match() } ) } } @@ -96,13 +95,13 @@ nextflow_process { } process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)] + ]) """ } } @@ -110,8 +109,7 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out.reads).match() }, - { assert path(process.out.versions.get(0)).getText().contains("cat") } + { assert snapshot(process.out).match() } ) } } @@ -124,10 +122,10 @@ nextflow_process { } process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:true ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)] + ]) """ } } @@ -135,8 +133,7 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out.reads).match() }, - { assert path(process.out.versions.get(0)).getText().contains("cat") } + { assert snapshot(process.out).match() } ) } } diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test.snap b/modules/nf-core/cat/fastq/tests/main.nf.test.snap index ec2342e5..43dfe28f 100644 --- a/modules/nf-core/cat/fastq/tests/main.nf.test.snap +++ b/modules/nf-core/cat/fastq/tests/main.nf.test.snap @@ -1,78 +1,169 @@ { "test_cat_fastq_single_end": { "content": [ - [ - [ - { - "id": "test", - "single_end": true - }, - "test.merged.fastq.gz:md5,f9cf5e375f7de81a406144a2c70cc64d" + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.merged.fastq.gz:md5,ee314a9bd568d06617171b0c85f508da" + ] + ], + "1": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" + ], + "reads": [ + [ + { + "id": "test", + "single_end": true + }, + "test.merged.fastq.gz:md5,ee314a9bd568d06617171b0c85f508da" + ] + ], + "versions": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" ] - ] + } ], - "timestamp": "2023-10-17T23:19:12.990284837" + "timestamp": "2024-01-17T17:30:39.816981" }, "test_cat_fastq_single_end_same_name": { "content": [ - [ - [ - { - "id": "test", - "single_end": true - }, - "test.merged.fastq.gz:md5,63f817db7a29a03eb538104495556f66" + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22" + ] + ], + "1": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" + ], + "reads": [ + [ + { + "id": "test", + "single_end": true + }, + "test.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22" + ] + ], + "versions": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" ] - ] + } ], - "timestamp": "2023-10-17T23:19:31.554568147" + "timestamp": "2024-01-17T17:32:35.229332" }, "test_cat_fastq_single_end_single_file": { "content": [ - [ - [ - { - "id": "test", - "single_end": true - }, - "test.merged.fastq.gz:md5,e325ef7deb4023447a1f074e285761af" + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.merged.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "1": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" + ], + "reads": [ + [ + { + "id": "test", + "single_end": true + }, + "test.merged.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "versions": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" ] - ] + } ], - "timestamp": "2023-10-17T23:19:49.629360033" + "timestamp": "2024-01-17T17:34:00.058829" }, "test_cat_fastq_paired_end_same_name": { "content": [ - [ - [ - { - "id": "test", - "single_end": false - }, + { + "0": [ [ - "test_1.merged.fastq.gz:md5,63f817db7a29a03eb538104495556f66", - "test_2.merged.fastq.gz:md5,fe9f266f43a6fc3dcab690a18419a56e" + { + "id": "test", + "single_end": false + }, + [ + "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22", + "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5" + ] ] + ], + "1": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" + ], + "reads": [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22", + "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5" + ] + ] + ], + "versions": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" ] - ] + } ], - "timestamp": "2023-10-17T23:19:40.711617539" + "timestamp": "2024-01-17T17:33:33.031555" }, "test_cat_fastq_paired_end": { "content": [ - [ - [ - { - "id": "test", - "single_end": false - }, + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22", + "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5" + ] + ] + ], + "1": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" + ], + "reads": [ [ - "test_1.merged.fastq.gz:md5,f9cf5e375f7de81a406144a2c70cc64d", - "test_2.merged.fastq.gz:md5,77c8e966e130d8c6b6ec9be52fcb2bda" + { + "id": "test", + "single_end": false + }, + [ + "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22", + "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5" + ] ] + ], + "versions": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" ] - ] + } ], - "timestamp": "2023-10-18T07:53:20.923560211" + "timestamp": "2024-01-17T17:32:02.270935" } } \ No newline at end of file diff --git a/modules/nf-core/cutadapt/cutadapt.diff b/modules/nf-core/cutadapt/cutadapt.diff index 5ccfbfe2..2816f8ac 100644 --- a/modules/nf-core/cutadapt/cutadapt.diff +++ b/modules/nf-core/cutadapt/cutadapt.diff @@ -2,7 +2,7 @@ Changes in module 'nf-core/cutadapt' --- modules/nf-core/cutadapt/main.nf +++ modules/nf-core/cutadapt/main.nf @@ -8,7 +8,7 @@ - 'biocontainers/cutadapt:3.4--py39h38f01e4_1' }" + 'biocontainers/cutadapt:4.6--py39hf95cd2a_1' }" input: - tuple val(meta), path(reads) @@ -10,11 +10,11 @@ Changes in module 'nf-core/cutadapt' output: tuple val(meta), path('*.trim.fastq.gz'), emit: reads -@@ -29,12 +29,13 @@ +@@ -30,12 +30,13 @@ $trimmed \\ $reads \\ > ${prefix}.cutadapt.log -+ ++ cat <<-END_VERSIONS > versions.yml "${task.process}": cutadapt: \$(cutadapt --version) diff --git a/modules/nf-core/cutadapt/environment.yml b/modules/nf-core/cutadapt/environment.yml index d32a8f97..288ea6f0 100644 --- a/modules/nf-core/cutadapt/environment.yml +++ b/modules/nf-core/cutadapt/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::cutadapt=3.4 + - bioconda::cutadapt=4.6 diff --git a/modules/nf-core/cutadapt/main.nf b/modules/nf-core/cutadapt/main.nf index 1a0145e4..128faa88 100644 --- a/modules/nf-core/cutadapt/main.nf +++ b/modules/nf-core/cutadapt/main.nf @@ -4,8 +4,8 @@ process CUTADAPT { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/cutadapt:3.4--py39h38f01e4_1' : - 'biocontainers/cutadapt:3.4--py39h38f01e4_1' }" + 'https://depot.galaxyproject.org/singularity/cutadapt:4.6--py39hf95cd2a_1' : + 'biocontainers/cutadapt:4.6--py39hf95cd2a_1' }" input: tuple val(meta), path(reads), path(adapter_seq) @@ -24,12 +24,13 @@ process CUTADAPT { def trimmed = meta.single_end ? "-o ${prefix}.trim.fastq.gz" : "-o ${prefix}_1.trim.fastq.gz -p ${prefix}_2.trim.fastq.gz" """ cutadapt \\ + -Z \\ --cores $task.cpus \\ $args \\ $trimmed \\ $reads \\ > ${prefix}.cutadapt.log - + cat <<-END_VERSIONS > versions.yml "${task.process}": cutadapt: \$(cutadapt --version) diff --git a/modules/nf-core/cutadapt/tests/main.nf.test b/modules/nf-core/cutadapt/tests/main.nf.test new file mode 100644 index 00000000..b7ea6ef9 --- /dev/null +++ b/modules/nf-core/cutadapt/tests/main.nf.test @@ -0,0 +1,69 @@ +nextflow_process { + + name "Test Process CUTADAPT" + script "../main.nf" + process "CUTADAPT" + tag "modules" + tag "modules_nfcore" + tag "cutadapt" + + test("sarscov2 Illumina single end [fastq]") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id: 'test', single_end:true ], + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert process.out.reads != null }, + { assert process.out.reads.get(0).get(1) ==~ ".*.trim.fastq.gz" }, + { assert snapshot(process.out.versions).match("versions_single_end") }, + { assert snapshot(path(process.out.reads.get(0).get(1)).linesGzip[0]).match() } + ) + } + } + + test("sarscov2 Illumina paired-end [fastq]") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id: 'test', single_end:false ], + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert process.out.reads != null }, + { assert process.out.reads.get(0).get(1).get(0) ==~ ".*.1.trim.fastq.gz" }, + { assert process.out.reads.get(0).get(1).get(1) ==~ ".*.2.trim.fastq.gz" }, + { assert snapshot(path(process.out.reads.get(0).get(1).get(1)).linesGzip[0]).match() }, + { assert snapshot(process.out.versions).match("versions_paired_end") } + ) + } + } +} diff --git a/modules/nf-core/cutadapt/tests/main.nf.test.snap b/modules/nf-core/cutadapt/tests/main.nf.test.snap new file mode 100644 index 00000000..3df7389e --- /dev/null +++ b/modules/nf-core/cutadapt/tests/main.nf.test.snap @@ -0,0 +1,46 @@ +{ + "sarscov2 Illumina single end [fastq]": { + "content": [ + "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-06T10:27:15.235936866" + }, + "sarscov2 Illumina paired-end [fastq]": { + "content": [ + "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/2" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-06T10:27:24.38468252" + }, + "versions_paired_end": { + "content": [ + [ + "versions.yml:md5,bc9892c68bfa7084ec5dbffbb9e8322f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-06T10:27:24.38799189" + }, + "versions_single_end": { + "content": [ + [ + "versions.yml:md5,bc9892c68bfa7084ec5dbffbb9e8322f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-06T10:27:15.219246449" + } +} \ No newline at end of file diff --git a/modules/nf-core/cutadapt/tests/nextflow.config b/modules/nf-core/cutadapt/tests/nextflow.config new file mode 100644 index 00000000..6c3b4253 --- /dev/null +++ b/modules/nf-core/cutadapt/tests/nextflow.config @@ -0,0 +1,7 @@ +process { + + withName: CUTADAPT { + ext.args = '-q 25' + } + +} diff --git a/modules/nf-core/cutadapt/tests/tags.yml b/modules/nf-core/cutadapt/tests/tags.yml new file mode 100644 index 00000000..f64f9975 --- /dev/null +++ b/modules/nf-core/cutadapt/tests/tags.yml @@ -0,0 +1,2 @@ +cutadapt: + - modules/nf-core/cutadapt/** diff --git a/modules/nf-core/minimap2/align/environment.yml b/modules/nf-core/minimap2/align/environment.yml index 60b9a8bf..051ca8ef 100644 --- a/modules/nf-core/minimap2/align/environment.yml +++ b/modules/nf-core/minimap2/align/environment.yml @@ -4,5 +4,6 @@ channels: - bioconda - defaults dependencies: - - bioconda::minimap2=2.24 - - bioconda::samtools=1.14 + - bioconda::minimap2=2.28 + - bioconda::samtools=1.19.2 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/minimap2/align/main.nf b/modules/nf-core/minimap2/align/main.nf index 54683a2c..0b1ae562 100644 --- a/modules/nf-core/minimap2/align/main.nf +++ b/modules/nf-core/minimap2/align/main.nf @@ -1,12 +1,12 @@ process MINIMAP2_ALIGN { tag "$meta.id" - label 'process_medium' + label 'process_high' // Note: the versions here need to match the versions used in the mulled container below and minimap2/index conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' : - 'biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' }" + 'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:3a70f8bc7e17b723591f6132418640cfdbc88246-0' : + 'biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:3a70f8bc7e17b723591f6132418640cfdbc88246-0' }" input: tuple val(meta), path(reads), path(reference) @@ -17,28 +17,43 @@ process MINIMAP2_ALIGN { output: tuple val(meta), path("*.paf"), optional: true, emit: paf tuple val(meta), path("*.bam"), optional: true, emit: bam + tuple val(meta), path("*.csi"), optional: true, emit: csi path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def bam_output = bam_format ? "-a | samtools sort | samtools view -@ ${task.cpus} -b -h -o ${prefix}.bam" : "-o ${prefix}.paf" + def bam_output = bam_format ? "-a | samtools sort -@ ${task.cpus-1} -o ${prefix}.bam ${args2}" : "-o ${prefix}.paf" def cigar_paf = cigar_paf_format && !bam_format ? "-c" : '' def set_cigar_bam = cigar_bam && bam_format ? "-L" : '' """ minimap2 \\ $args \\ -t $task.cpus \\ - "${reference ?: reads}" \\ - "$reads" \\ + ${reference ?: reads} \\ + $reads \\ $cigar_paf \\ $set_cigar_bam \\ $bam_output + cat <<-END_VERSIONS > versions.yml + "${task.process}": + minimap2: \$(minimap2 --version 2>&1) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def output_file = bam_format ? "${prefix}.bam" : "${prefix}.paf" + """ + touch $output_file + touch ${prefix}.csi + cat <<-END_VERSIONS > versions.yml "${task.process}": minimap2: \$(minimap2 --version 2>&1) diff --git a/modules/nf-core/minimap2/align/minimap2-align.diff b/modules/nf-core/minimap2/align/minimap2-align.diff index 6faf1573..13a12457 100644 --- a/modules/nf-core/minimap2/align/minimap2-align.diff +++ b/modules/nf-core/minimap2/align/minimap2-align.diff @@ -1,4 +1,17 @@ Changes in module 'nf-core/minimap2/align' +--- modules/nf-core/minimap2/align/main.nf ++++ modules/nf-core/minimap2/align/main.nf +@@ -9,8 +9,7 @@ + 'biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:3a70f8bc7e17b723591f6132418640cfdbc88246-0' }" + + input: +- tuple val(meta), path(reads) +- tuple val(meta2), path(reference) ++ tuple val(meta), path(reads), path(reference) + val bam_format + val cigar_paf_format + val cigar_bam + --- modules/nf-core/minimap2/align/meta.yml +++ modules/nf-core/minimap2/align/meta.yml @@ -25,11 +25,6 @@ @@ -14,17 +27,4 @@ Changes in module 'nf-core/minimap2/align' type: file description: | ---- modules/nf-core/minimap2/align/main.nf -+++ modules/nf-core/minimap2/align/main.nf -@@ -9,8 +9,7 @@ - 'biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' }" - - input: -- tuple val(meta), path(reads) -- tuple val(meta2), path(reference) -+ tuple val(meta), path(reads), path(reference) - val bam_format - val cigar_paf_format - val cigar_bam - ************************************************************ diff --git a/modules/nf-core/minimap2/align/tests/main.nf.test b/modules/nf-core/minimap2/align/tests/main.nf.test new file mode 100644 index 00000000..83cceeab --- /dev/null +++ b/modules/nf-core/minimap2/align/tests/main.nf.test @@ -0,0 +1,181 @@ +nextflow_process { + + name "Test Process MINIMAP2_ALIGN" + script "../main.nf" + process "MINIMAP2_ALIGN" + + tag "modules" + tag "modules_nfcore" + tag "minimap2" + tag "minimap2/align" + + test("sarscov2 - fastq, fasta, true, false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = true + input[3] = false + input[4] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], fasta, true, false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = true + input[3] = false + input[4] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, [], true, false, false") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + [] + ] + input[2] = true + input[3] = false + input[4] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, fasta, true, false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = true + input[3] = false + input[4] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + file(process.out.csi[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, fasta, false, false, false - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = [ + [ id:'test_ref' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = false + input[3] = false + input[4] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.paf[0][1]).name, + file(process.out.csi[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + +} \ No newline at end of file diff --git a/modules/nf-core/minimap2/align/tests/main.nf.test.snap b/modules/nf-core/minimap2/align/tests/main.nf.test.snap new file mode 100644 index 00000000..19a8f204 --- /dev/null +++ b/modules/nf-core/minimap2/align/tests/main.nf.test.snap @@ -0,0 +1,69 @@ +{ + "sarscov2 - fastq, fasta, true, false, false": { + "content": [ + "test.bam", + [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-05T10:14:18.939731126" + }, + "sarscov2 - fastq, fasta, true, false, false - stub": { + "content": [ + "test.bam", + "test.csi", + [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-05T10:14:34.275879844" + }, + "sarscov2 - fastq, fasta, false, false, false - stub": { + "content": [ + "test.paf", + "test.csi", + [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-05T10:14:39.227958138" + }, + "sarscov2 - [fastq1, fastq2], fasta, true, false, false": { + "content": [ + "test.bam", + [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-05T10:14:24.265054877" + }, + "sarscov2 - fastq, [], true, false, false": { + "content": [ + "test.bam", + [ + "versions.yml:md5,98b8f5f36aa54b82210094f0b0d11938" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-05T10:14:29.27901773" + } +} \ No newline at end of file diff --git a/modules/nf-core/minimap2/align/tests/tags.yml b/modules/nf-core/minimap2/align/tests/tags.yml new file mode 100644 index 00000000..39dba374 --- /dev/null +++ b/modules/nf-core/minimap2/align/tests/tags.yml @@ -0,0 +1,2 @@ +minimap2/align: + - "modules/nf-core/minimap2/align/**" diff --git a/modules/nf-core/minimap2/index/environment.yml b/modules/nf-core/minimap2/index/environment.yml index 2a66e410..8a912a12 100644 --- a/modules/nf-core/minimap2/index/environment.yml +++ b/modules/nf-core/minimap2/index/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::minimap2=2.24 + - bioconda::minimap2=2.28 diff --git a/modules/nf-core/minimap2/index/main.nf b/modules/nf-core/minimap2/index/main.nf index 45e1cec0..38320214 100644 --- a/modules/nf-core/minimap2/index/main.nf +++ b/modules/nf-core/minimap2/index/main.nf @@ -1,11 +1,11 @@ process MINIMAP2_INDEX { - label 'process_medium' + label 'process_low' // Note: the versions here need to match the versions used in minimap2/align conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/minimap2:2.24--h7132678_1' : - 'biocontainers/minimap2:2.24--h7132678_1' }" + 'https://depot.galaxyproject.org/singularity/minimap2:2.28--he4a0461_0' : + 'biocontainers/minimap2:2.28--he4a0461_0' }" input: tuple val(meta), path(fasta) @@ -31,4 +31,14 @@ process MINIMAP2_INDEX { minimap2: \$(minimap2 --version 2>&1) END_VERSIONS """ + + stub: + """ + touch ${fasta.baseName}.mmi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + minimap2: \$(minimap2 --version 2>&1) + END_VERSIONS + """ } diff --git a/modules/nf-core/minimap2/index/tests/main.nf.test b/modules/nf-core/minimap2/index/tests/main.nf.test new file mode 100644 index 00000000..97840ff7 --- /dev/null +++ b/modules/nf-core/minimap2/index/tests/main.nf.test @@ -0,0 +1,32 @@ +nextflow_process { + + name "Test Process MINIMAP2_INDEX" + script "../main.nf" + process "MINIMAP2_INDEX" + + tag "modules" + tag "modules_nfcore" + tag "minimap2" + tag "minimap2/index" + + test("minimap2 index") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + """ + } + } + + then { + assert process.success + assert snapshot(process.out).match() + } + + } + +} \ No newline at end of file diff --git a/modules/nf-core/minimap2/index/tests/main.nf.test.snap b/modules/nf-core/minimap2/index/tests/main.nf.test.snap new file mode 100644 index 00000000..0b098828 --- /dev/null +++ b/modules/nf-core/minimap2/index/tests/main.nf.test.snap @@ -0,0 +1,68 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + [ + { + "id": "test_ref" + }, + "genome.mmi:md5,72e450f12dc691e763c697463bdb1571" + ] + ], + "1": [ + "versions.yml:md5,0fced0ee8015e7f50b82566e3db8f7b0" + ], + "index": [ + [ + { + "id": "test_ref" + }, + "genome.mmi:md5,72e450f12dc691e763c697463bdb1571" + ] + ], + "versions": [ + "versions.yml:md5,0fced0ee8015e7f50b82566e3db8f7b0" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T11:46:30.000058092" + }, + "minimap2 index": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "genome.mmi:md5,72e450f12dc691e763c697463bdb1571" + ] + ], + "1": [ + "versions.yml:md5,2f8340380c6741e9261a284262a90bde" + ], + "index": [ + [ + { + "id": "test" + }, + "genome.mmi:md5,72e450f12dc691e763c697463bdb1571" + ] + ], + "versions": [ + "versions.yml:md5,2f8340380c6741e9261a284262a90bde" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-05T10:58:29.828187662" + } +} \ No newline at end of file diff --git a/modules/nf-core/minimap2/index/tests/tags.yml b/modules/nf-core/minimap2/index/tests/tags.yml new file mode 100644 index 00000000..e5ef8e19 --- /dev/null +++ b/modules/nf-core/minimap2/index/tests/tags.yml @@ -0,0 +1,2 @@ +minimap2/index: + - modules/nf-core/minimap2/index/** diff --git a/modules/nf-core/racon/tests/main.nf.test b/modules/nf-core/racon/tests/main.nf.test new file mode 100644 index 00000000..1f97fb95 --- /dev/null +++ b/modules/nf-core/racon/tests/main.nf.test @@ -0,0 +1,34 @@ +nextflow_process { + + name "Test Process RACON" + script "../main.nf" + process "RACON" + + tag "modules" + tag "modules_nfcore" + tag "racon" + + test("single-end - bacteroides_fragilis - [fastq_gz, fna_gz, paf]") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['bacteroides_fragilis']['nanopore']['test_fastq_gz'], checkIfExists: true), + file(params.test_data['bacteroides_fragilis']['genome']['genome_fna_gz'], checkIfExists: true), + file(params.test_data['bacteroides_fragilis']['genome']['genome_paf'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } +} \ No newline at end of file diff --git a/modules/nf-core/racon/tests/main.nf.test.snap b/modules/nf-core/racon/tests/main.nf.test.snap new file mode 100644 index 00000000..1ac00d5d --- /dev/null +++ b/modules/nf-core/racon/tests/main.nf.test.snap @@ -0,0 +1,37 @@ +{ + "single-end - bacteroides_fragilis - [fastq_gz, fna_gz, paf]": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test_assembly_consensus.fasta.gz:md5,e610bf2e1df1701b88675e36a844baf9" + ] + ], + "1": [ + "versions.yml:md5,7eeab4e50acdc04c648149c2b5a01c84" + ], + "improved_assembly": [ + [ + { + "id": "test", + "single_end": true + }, + "test_assembly_consensus.fasta.gz:md5,e610bf2e1df1701b88675e36a844baf9" + ] + ], + "versions": [ + "versions.yml:md5,7eeab4e50acdc04c648149c2b5a01c84" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.1" + }, + "timestamp": "2024-05-23T11:31:56.975593" + } +} \ No newline at end of file diff --git a/modules/nf-core/racon/tests/tags.yml b/modules/nf-core/racon/tests/tags.yml new file mode 100644 index 00000000..5b883e80 --- /dev/null +++ b/modules/nf-core/racon/tests/tags.yml @@ -0,0 +1,2 @@ +racon: + - modules/nf-core/racon/** diff --git a/modules/nf-core/samtools/index/environment.yml b/modules/nf-core/samtools/index/environment.yml index 3c6f95b2..a5e50649 100644 --- a/modules/nf-core/samtools/index/environment.yml +++ b/modules/nf-core/samtools/index/environment.yml @@ -4,4 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::samtools=1.17 + - bioconda::samtools=1.19.2 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf index 256bd7c4..dc14f98d 100644 --- a/modules/nf-core/samtools/index/main.nf +++ b/modules/nf-core/samtools/index/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_INDEX { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : + 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" input: tuple val(meta), path(input) diff --git a/modules/nf-core/samtools/index/tests/main.nf.test b/modules/nf-core/samtools/index/tests/main.nf.test index c76a9169..bb7756d1 100644 --- a/modules/nf-core/samtools/index/tests/main.nf.test +++ b/modules/nf-core/samtools/index/tests/main.nf.test @@ -8,7 +8,7 @@ nextflow_process { tag "samtools" tag "samtools/index" - test("sarscov2 [BAI]") { + test("bai") { when { params { @@ -16,10 +16,10 @@ nextflow_process { } process { """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) """ } } @@ -28,12 +28,12 @@ nextflow_process { assertAll ( { assert process.success }, { assert snapshot(process.out.bai).match("bai") }, - { assert path(process.out.versions.get(0)).getText().contains("samtools") } + { assert snapshot(process.out.versions).match("bai_versions") } ) } } - test("homo_sapiens [CRAI]") { + test("crai") { when { params { @@ -41,10 +41,10 @@ nextflow_process { } process { """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true) - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true) + ]) """ } } @@ -53,12 +53,12 @@ nextflow_process { assertAll ( { assert process.success }, { assert snapshot(process.out.crai).match("crai") }, - { assert path(process.out.versions.get(0)).getText().contains("samtools") } + { assert snapshot(process.out.versions).match("crai_versions") } ) } } - test("homo_sapiens [CSI]") { + test("csi") { config "./csi.nextflow.config" @@ -68,10 +68,10 @@ nextflow_process { } process { """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) """ } } @@ -80,7 +80,7 @@ nextflow_process { assertAll ( { assert process.success }, { assert path(process.out.csi.get(0).get(1)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("samtools") } + { assert snapshot(process.out.versions).match("csi_versions") } ) } } diff --git a/modules/nf-core/samtools/index/tests/main.nf.test.snap b/modules/nf-core/samtools/index/tests/main.nf.test.snap index b3baee7f..3dc8e7de 100644 --- a/modules/nf-core/samtools/index/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/index/tests/main.nf.test.snap @@ -1,28 +1,74 @@ { + "crai_versions": { + "content": [ + [ + "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:12:00.324667957" + }, + "csi_versions": { + "content": [ + [ + "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:12:07.885103162" + }, "crai": { "content": [ [ [ { - "id": "test" + "id": "test", + "single_end": false }, "test.paired_end.recalibrated.sorted.cram.crai:md5,14bc3bd5c89cacc8f4541f9062429029" ] ] ], - "timestamp": "2023-11-15T15:17:37.30801" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:41:38.446424" }, "bai": { "content": [ [ [ { - "id": "test" + "id": "test", + "single_end": false }, "test.paired_end.sorted.bam.bai:md5,704c10dd1326482448ca3073fdebc2f4" ] ] ], - "timestamp": "2023-11-15T15:17:30.869234" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-12T18:40:46.579747" + }, + "bai_versions": { + "content": [ + [ + "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:11:51.641425452" } } \ No newline at end of file diff --git a/modules/nf-core/seqtk/seq/main.nf b/modules/nf-core/seqtk/seq/main.nf index af085f0d..3199f7ec 100644 --- a/modules/nf-core/seqtk/seq/main.nf +++ b/modules/nf-core/seqtk/seq/main.nf @@ -37,4 +37,20 @@ process SEQTK_SEQ { seqtk: \$(echo \$(seqtk 2>&1) | sed 's/^.*Version: //; s/ .*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + + def extension = "fastq" + if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz/ || "$args" ==~ /\-[aA]/ ) { + extension = "fasta" + } + """ + echo "" | gzip > ${prefix}.seqtk-seq.${extension}.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + seqtk: \$(echo \$(seqtk 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/seqtk/seq/tests/main.nf.test b/modules/nf-core/seqtk/seq/tests/main.nf.test new file mode 100644 index 00000000..82e0a0ce --- /dev/null +++ b/modules/nf-core/seqtk/seq/tests/main.nf.test @@ -0,0 +1,88 @@ +nextflow_process { + + name "Test Process SEQTK_SEQ" + script "modules/nf-core/seqtk/seq/main.nf" + process "SEQTK_SEQ" + config "./standard.config" + + tag "modules" + tag "modules_nfcore" + tag "seqtk" + tag "seqtk/seq" + + test("sarscov2_seq_fa") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.fastx[0][1]).name + ).match("seq_fa") + } + ) + } + + } + + test("sarscov2_seq_fq") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.fastx[0][1]).name + ).match("seq_fq") + } + ) + } + + } + + test("sarscov2_seq_fa_stub") { + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/seqtk/seq/tests/main.nf.test.snap b/modules/nf-core/seqtk/seq/tests/main.nf.test.snap new file mode 100644 index 00000000..2940cde1 --- /dev/null +++ b/modules/nf-core/seqtk/seq/tests/main.nf.test.snap @@ -0,0 +1,101 @@ +{ + "sarscov2_seq_fa": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.seqtk-seq.fasta.gz:md5,e73599798195a519ba2565c3f0275b93" + ] + ], + "1": [ + "versions.yml:md5,d3214d3bd8c0bb57f06550146eab94fa" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.seqtk-seq.fasta.gz:md5,e73599798195a519ba2565c3f0275b93" + ] + ], + "versions": [ + "versions.yml:md5,d3214d3bd8c0bb57f06550146eab94fa" + ] + } + ], + "timestamp": "2024-02-22T15:57:07.883846" + }, + "sarscov2_seq_fq": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.seqtk-seq.fasta.gz:md5,f0c5c9110ce19e9ebbc9a6b6baf9e105" + ] + ], + "1": [ + "versions.yml:md5,d3214d3bd8c0bb57f06550146eab94fa" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.seqtk-seq.fasta.gz:md5,f0c5c9110ce19e9ebbc9a6b6baf9e105" + ] + ], + "versions": [ + "versions.yml:md5,d3214d3bd8c0bb57f06550146eab94fa" + ] + } + ], + "timestamp": "2024-02-22T15:57:16.282429" + }, + "sarscov2_seq_fa_stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.seqtk-seq.fasta.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + "versions.yml:md5,d3214d3bd8c0bb57f06550146eab94fa" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.seqtk-seq.fasta.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,d3214d3bd8c0bb57f06550146eab94fa" + ] + } + ], + "timestamp": "2024-02-22T15:57:24.751443" + }, + "seq_fa": { + "content": [ + "test.seqtk-seq.fasta.gz" + ], + "timestamp": "2024-02-22T15:57:07.936813" + }, + "seq_fq": { + "content": [ + "test.seqtk-seq.fasta.gz" + ], + "timestamp": "2024-02-22T15:57:16.311642" + } +} \ No newline at end of file diff --git a/modules/nf-core/seqtk/seq/tests/standard.config b/modules/nf-core/seqtk/seq/tests/standard.config new file mode 100644 index 00000000..beeffb97 --- /dev/null +++ b/modules/nf-core/seqtk/seq/tests/standard.config @@ -0,0 +1,5 @@ +process { + withName: 'SEQTK_SEQ' { + ext.args = '-A' + } +} \ No newline at end of file diff --git a/modules/nf-core/seqtk/seq/tests/tags.yml b/modules/nf-core/seqtk/seq/tests/tags.yml new file mode 100644 index 00000000..d237d869 --- /dev/null +++ b/modules/nf-core/seqtk/seq/tests/tags.yml @@ -0,0 +1,2 @@ +seqtk/seq: + - "modules/nf-core/seqtk/seq/**" diff --git a/modules/nf-core/vsearch/cluster/tests/main.nf.test b/modules/nf-core/vsearch/cluster/tests/main.nf.test new file mode 100644 index 00000000..1a376793 --- /dev/null +++ b/modules/nf-core/vsearch/cluster/tests/main.nf.test @@ -0,0 +1,132 @@ +nextflow_process { + + name "Test Process VSEARCH_CLUSTER" + script "../main.nf" + process "VSEARCH_CLUSTER" + + tag "modules" + tag "modules_nfcore" + tag "vsearch" + tag "vsearch/cluster" + + test("sarscov2 - fastq - cluster fast") { + + config './nextflow_fast.config' + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - fastq - cluster size") { + + config './nextflow_size.config' + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - fastq - cluster smallmem") { + + config './nextflow_smallmem.config' + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - fastq - cluster unoise") { + + config './nextflow_unoise.config' + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - fastq - cluster userout") { + + config './nextflow_userout.config' + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + 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b/modules/nf-core/vsearch/cluster/tests/nextflow_fast.config new file mode 100644 index 00000000..df76a060 --- /dev/null +++ b/modules/nf-core/vsearch/cluster/tests/nextflow_fast.config @@ -0,0 +1,7 @@ +process { + + ext.args = '--id 0.8' + ext.args2 = "--cluster_fast" + ext.args3 = "--centroids" + +} diff --git a/modules/nf-core/vsearch/cluster/tests/nextflow_size.config b/modules/nf-core/vsearch/cluster/tests/nextflow_size.config new file mode 100644 index 00000000..b650043e --- /dev/null +++ b/modules/nf-core/vsearch/cluster/tests/nextflow_size.config @@ -0,0 +1,7 @@ +process { + + ext.args = '--id 0.8' + ext.args2 = "--cluster_size" + ext.args3 = "--samout" // Test also sam to bam conversion + +} diff --git a/modules/nf-core/vsearch/cluster/tests/nextflow_smallmem.config b/modules/nf-core/vsearch/cluster/tests/nextflow_smallmem.config new file mode 100644 index 00000000..5e4efb8b --- /dev/null +++ b/modules/nf-core/vsearch/cluster/tests/nextflow_smallmem.config @@ -0,0 +1,7 @@ +process { + + ext.args = '--id 0.8 --usersort' + ext.args2 = "--cluster_smallmem" + ext.args3 = "--centroids" + +} diff --git a/modules/nf-core/vsearch/cluster/tests/nextflow_unoise.config b/modules/nf-core/vsearch/cluster/tests/nextflow_unoise.config new file mode 100644 index 00000000..96ccf725 --- /dev/null +++ b/modules/nf-core/vsearch/cluster/tests/nextflow_unoise.config @@ -0,0 +1,7 @@ +process { + + ext.args = '--id 0.8 --minsize 1' + ext.args2 = "--cluster_unoise" + ext.args3 = "--centroids" + +} diff --git a/modules/nf-core/vsearch/cluster/tests/nextflow_userout.config b/modules/nf-core/vsearch/cluster/tests/nextflow_userout.config new file mode 100644 index 00000000..63b04555 --- /dev/null +++ b/modules/nf-core/vsearch/cluster/tests/nextflow_userout.config @@ -0,0 +1,7 @@ +process { + + ext.args = '--id 0.8 --userfields query+target+id' + ext.args2 = "--cluster_fast" + ext.args3 = "--userout" + +} diff --git a/modules/nf-core/vsearch/cluster/tests/tags.yml b/modules/nf-core/vsearch/cluster/tests/tags.yml new file mode 100644 index 00000000..7238024f --- /dev/null +++ b/modules/nf-core/vsearch/cluster/tests/tags.yml @@ -0,0 +1,2 @@ +vsearch/cluster: + - "modules/nf-core/vsearch/cluster/**" diff --git a/modules/nf-core/vsearch/sort/tests/main.nf.test b/modules/nf-core/vsearch/sort/tests/main.nf.test new file mode 100644 index 00000000..db043851 --- /dev/null +++ b/modules/nf-core/vsearch/sort/tests/main.nf.test @@ -0,0 +1,60 @@ +nextflow_process { + + name "Test Process VSEARCH_SORT" + script "../main.nf" + process "VSEARCH_SORT" + + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "vsearch" + tag "vsearch/sort" + + test("vsearch-sort - sort - size") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = "--sortbysize" + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("vsearch-sort - sort - length") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = "--sortbylength" + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/vsearch/sort/tests/main.nf.test.snap b/modules/nf-core/vsearch/sort/tests/main.nf.test.snap new file mode 100644 index 00000000..0a278d10 --- /dev/null +++ b/modules/nf-core/vsearch/sort/tests/main.nf.test.snap @@ -0,0 +1,72 @@ +{ + "vsearch-sort - sort - size": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test_sorted.fasta:md5,86457b12184a7215734ff1e4d868ac51" + ] + ], + "1": [ + "versions.yml:md5,19dba7cb81af6ce4b80f3bf99829ee9d" + ], + "fasta": [ + [ + { + "id": "test", + "single_end": false + }, + "test_sorted.fasta:md5,86457b12184a7215734ff1e4d868ac51" + ] + ], + "versions": [ + "versions.yml:md5,19dba7cb81af6ce4b80f3bf99829ee9d" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.1" + }, + "timestamp": "2024-05-24T13:05:54.524638" + }, + "vsearch-sort - sort - length": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test_sorted.fasta:md5,e733a34d0c655966408e773de876e5fb" + ] + ], + "1": [ + "versions.yml:md5,19dba7cb81af6ce4b80f3bf99829ee9d" + ], + "fasta": [ + [ + { + "id": "test", + "single_end": false + }, + "test_sorted.fasta:md5,e733a34d0c655966408e773de876e5fb" + ] + ], + "versions": [ + "versions.yml:md5,19dba7cb81af6ce4b80f3bf99829ee9d" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.1" + }, + "timestamp": "2024-05-24T13:06:01.210745" + } +} \ No newline at end of file diff --git a/modules/nf-core/vsearch/sort/tests/nextflow.config b/modules/nf-core/vsearch/sort/tests/nextflow.config new file mode 100644 index 00000000..7b3f6a9c --- /dev/null +++ b/modules/nf-core/vsearch/sort/tests/nextflow.config @@ -0,0 +1,6 @@ +process { + + publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" } + ext.prefix = { "${meta.id}_sorted" } + +} diff --git a/modules/nf-core/vsearch/sort/tests/tags.yml b/modules/nf-core/vsearch/sort/tests/tags.yml new file mode 100644 index 00000000..d7220281 --- /dev/null +++ b/modules/nf-core/vsearch/sort/tests/tags.yml @@ -0,0 +1,2 @@ +vsearch/sort: + - modules/nf-core/vsearch/sort/** From db7c406aca4f741f840503f94d7c4768715f90b1 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Mon, 27 May 2024 10:18:04 +0000 Subject: [PATCH 180/226] change pipelines_testdata_base_path default --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 04f35445..e3b7e580 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -463,7 +463,7 @@ "type": "string", "fa_icon": "far fa-check-circle", "description": "Base URL or local path to location of pipeline test dataset files", - "default": "https://raw.githubusercontent.com/nf-core/test-datasets/", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/cirsprseq/", "hidden": true } } From 849c4deffa3a69297e8b81698fc83e209dd20da2 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Mon, 27 May 2024 10:29:13 +0000 Subject: [PATCH 181/226] use pipelines_testdata_base_path in test configs and don't add crisprseq to the param path --- conf/test_screening.config | 6 +++--- conf/test_screening_full.config | 6 +++--- conf/test_screening_paired.config | 4 ++-- conf/test_screening_rra.config | 6 +++--- conf/test_targeted.config | 2 +- conf/test_targeted_full.config | 2 +- conf/test_umis.config | 2 +- nextflow.config | 2 +- nextflow_schema.json | 2 +- 9 files changed, 16 insertions(+), 16 deletions(-) diff --git a/conf/test_screening.config b/conf/test_screening.config index 8a6cfc9e..8cdad143 100644 --- a/conf/test_screening.config +++ b/conf/test_screening.config @@ -20,11 +20,11 @@ params { max_time = '6.h' // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/samplesheet_test.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata/samplesheet_test.csv' analysis = 'screening' crisprcleanr = "Brunello_Library" - library = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/brunello_target_sequence.txt" - contrasts = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/rra_contrasts.txt" + library = params.pipelines_testdata_base_path + "crisprseq/testdata/brunello_target_sequence.txt" + contrasts = params.pipelines_testdata_base_path + "crisprseq/testdata/rra_contrasts.txt" } process { diff --git a/conf/test_screening_full.config b/conf/test_screening_full.config index 21709de9..8a1f37e7 100644 --- a/conf/test_screening_full.config +++ b/conf/test_screening_full.config @@ -15,11 +15,11 @@ params { config_profile_description = 'Full test dataset to check pipeline function' // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/full_test/samplesheet_full.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata/full_test/samplesheet_full.csv' analysis = 'screening' crisprcleanr = "Brunello_Library" - mle_design_matrix = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/full_test/drugA_drugB_vs_treatment.txt" - library = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/brunello_target_sequence.txt" + mle_design_matrix = params.pipelines_testdata_base_path + "crisprseq/testdata/full_test/drugA_drugB_vs_treatment.txt" + library = params.pipelines_testdata_base_path + "crisprseq/testdata/brunello_target_sequence.txt" } process { diff --git a/conf/test_screening_paired.config b/conf/test_screening_paired.config index 90867efd..a01d026b 100644 --- a/conf/test_screening_paired.config +++ b/conf/test_screening_paired.config @@ -20,9 +20,9 @@ params { max_time = '6.h' // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/samplesheet_test_paired.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata/samplesheet_test_paired.csv' analysis = 'screening' - library = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/brunello_target_sequence.txt" + library = params.pipelines_testdata_base_path + "crisprseq/testdata/brunello_target_sequence.txt" } process { diff --git a/conf/test_screening_rra.config b/conf/test_screening_rra.config index bdda3a07..bde9dfce 100644 --- a/conf/test_screening_rra.config +++ b/conf/test_screening_rra.config @@ -20,11 +20,11 @@ params { max_time = '6.h' // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/samplesheet_test.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata/samplesheet_test.csv' analysis = 'screening' crisprcleanr = "Brunello_Library" - library = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/brunello_target_sequence.txt" - contrasts = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/rra_contrasts.txt" + library = params.pipelines_testdata_base_path + "crisprseq/testdata/brunello_target_sequence.txt" + contrasts = params.pipelines_testdata_base_path + "crisprseq/testdata/rra_contrasts.txt" rra = true } diff --git a/conf/test_targeted.config b/conf/test_targeted.config index 9bf6cabc..57f96e98 100644 --- a/conf/test_targeted.config +++ b/conf/test_targeted.config @@ -20,7 +20,7 @@ params { max_time = '6.h' // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata-edition/samplesheet_test.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata-edition/samplesheet_test.csv' analysis = 'targeted' // Aligner diff --git a/conf/test_targeted_full.config b/conf/test_targeted_full.config index f3622137..89b29f05 100644 --- a/conf/test_targeted_full.config +++ b/conf/test_targeted_full.config @@ -15,7 +15,7 @@ params { config_profile_description = 'Full test dataset to check pipeline function' // Input data for full size test - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata-edition/samplesheet_test_full.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata-edition/samplesheet_test_full.csv' analysis = 'targeted' // Aligner diff --git a/conf/test_umis.config b/conf/test_umis.config index d99effcb..0fe58f3b 100644 --- a/conf/test_umis.config +++ b/conf/test_umis.config @@ -20,7 +20,7 @@ params { max_time = '6.h' // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata-edition/samplesheet_test_umis.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata-edition/samplesheet_test_umis.csv' analysis = 'targeted' umi_clustering = true diff --git a/nextflow.config b/nextflow.config index 46a7701b..6fe4ef9c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -64,7 +64,7 @@ params { hook_url = null help = false version = false - pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/' + pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' // Config options config_profile_name = null diff --git a/nextflow_schema.json b/nextflow_schema.json index e3b7e580..04f35445 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -463,7 +463,7 @@ "type": "string", "fa_icon": "far fa-check-circle", "description": "Base URL or local path to location of pipeline test dataset files", - "default": "https://raw.githubusercontent.com/nf-core/test-datasets/cirsprseq/", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/", "hidden": true } } From 7efbadc95278a79040b863e68e779db2d9f85642 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Mon, 27 May 2024 10:31:03 +0000 Subject: [PATCH 182/226] spacing --- nextflow.config | 16 ++++++++-------- 1 file changed, 8 insertions(+), 8 deletions(-) diff --git a/nextflow.config b/nextflow.config index 6fe4ef9c..e25d2aba 100644 --- a/nextflow.config +++ b/nextflow.config @@ -133,14 +133,14 @@ profiles { apptainer.enabled = false } docker { - docker.enabled = true - conda.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false - process.containerOptions = '-u $(id -u):$(id -g)' + docker.enabled = true + conda.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false + process.containerOptions = '-u $(id -u):$(id -g)' } arm { process.containerOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' From 60da01130d7ff81379af96a099a39f20f8210998 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 13:08:54 +0200 Subject: [PATCH 183/226] Update conf/test_screening_rra.config --- conf/test_screening_rra.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_screening_rra.config b/conf/test_screening_rra.config index bde9dfce..fb6b9d3f 100644 --- a/conf/test_screening_rra.config +++ b/conf/test_screening_rra.config @@ -20,7 +20,7 @@ params { max_time = '6.h' // Input data - input = params.pipelines_testdata_base_path + "crisprseq/testdata/samplesheet_test.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata/samplesheet_test.csv" analysis = 'screening' crisprcleanr = "Brunello_Library" library = params.pipelines_testdata_base_path + "crisprseq/testdata/brunello_target_sequence.txt" From cf639295df9693f60b5a097b3f1b5e126007943b Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 13:09:29 +0200 Subject: [PATCH 184/226] Update conf/test_screening.config --- conf/test_screening.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_screening.config b/conf/test_screening.config index 8cdad143..412271a0 100644 --- a/conf/test_screening.config +++ b/conf/test_screening.config @@ -20,7 +20,7 @@ params { max_time = '6.h' // Input data - input = params.pipelines_testdata_base_path + "crisprseq/testdata/samplesheet_test.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata/samplesheet_test.csv" analysis = 'screening' crisprcleanr = "Brunello_Library" library = params.pipelines_testdata_base_path + "crisprseq/testdata/brunello_target_sequence.txt" From f31b13235713b0f37dc5cc93d454c260daff6567 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 13:09:52 +0200 Subject: [PATCH 185/226] Update conf/test_screening_full.config --- conf/test_screening_full.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_screening_full.config b/conf/test_screening_full.config index 8a1f37e7..2f74238c 100644 --- a/conf/test_screening_full.config +++ b/conf/test_screening_full.config @@ -15,7 +15,7 @@ params { config_profile_description = 'Full test dataset to check pipeline function' // Input data - input = params.pipelines_testdata_base_path + "crisprseq/testdata/full_test/samplesheet_full.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata/full_test/samplesheet_full.csv" analysis = 'screening' crisprcleanr = "Brunello_Library" mle_design_matrix = params.pipelines_testdata_base_path + "crisprseq/testdata/full_test/drugA_drugB_vs_treatment.txt" From 7fc91ee751f9295dc36ce8e2822f0e5223a73aaa Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 13:10:19 +0200 Subject: [PATCH 186/226] Update conf/test_screening_paired.config --- conf/test_screening_paired.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_screening_paired.config b/conf/test_screening_paired.config index a01d026b..7ff0a45d 100644 --- a/conf/test_screening_paired.config +++ b/conf/test_screening_paired.config @@ -20,7 +20,7 @@ params { max_time = '6.h' // Input data - input = params.pipelines_testdata_base_path + "crisprseq/testdata/samplesheet_test_paired.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata/samplesheet_test_paired.csv" analysis = 'screening' library = params.pipelines_testdata_base_path + "crisprseq/testdata/brunello_target_sequence.txt" } From 1733c777cdd90194409dbac6723905af0a7e985a Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 13:10:43 +0200 Subject: [PATCH 187/226] Update conf/test_targeted.config --- conf/test_targeted.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_targeted.config b/conf/test_targeted.config index 57f96e98..1906efb2 100644 --- a/conf/test_targeted.config +++ b/conf/test_targeted.config @@ -20,7 +20,7 @@ params { max_time = '6.h' // Input data - input = params.pipelines_testdata_base_path + "crisprseq/testdata-edition/samplesheet_test.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata-edition/samplesheet_test.csv" analysis = 'targeted' // Aligner From ff3fde026a64ebfd3d52850cfbe5d7ebe3fea8cf Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 13:11:08 +0200 Subject: [PATCH 188/226] Update conf/test_targeted_full.config --- conf/test_targeted_full.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_targeted_full.config b/conf/test_targeted_full.config index 89b29f05..ff6f1c17 100644 --- a/conf/test_targeted_full.config +++ b/conf/test_targeted_full.config @@ -15,7 +15,7 @@ params { config_profile_description = 'Full test dataset to check pipeline function' // Input data for full size test - input = params.pipelines_testdata_base_path + "crisprseq/testdata-edition/samplesheet_test_full.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata-edition/samplesheet_test_full.csv" analysis = 'targeted' // Aligner From e92523ba605b8d3039fa053f2f8e05d6c33e8f8f Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 13:11:36 +0200 Subject: [PATCH 189/226] Update conf/test_umis.config --- conf/test_umis.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_umis.config b/conf/test_umis.config index 0fe58f3b..d77b71f8 100644 --- a/conf/test_umis.config +++ b/conf/test_umis.config @@ -20,7 +20,7 @@ params { max_time = '6.h' // Input data - input = params.pipelines_testdata_base_path + "crisprseq/testdata-edition/samplesheet_test_umis.csv' + input = params.pipelines_testdata_base_path + "crisprseq/testdata-edition/samplesheet_test_umis.csv" analysis = 'targeted' umi_clustering = true From 5c7895be07eb38e4ba567d4b3e163ba2ec873cd1 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 27 May 2024 12:33:54 +0000 Subject: [PATCH 190/226] [automated] Fix linting with Prettier --- tower.yml | 1 - 1 file changed, 1 deletion(-) diff --git a/tower.yml b/tower.yml index a8d4cf52..03cbd716 100644 --- a/tower.yml +++ b/tower.yml @@ -33,4 +33,3 @@ reports: display: "reads" "cigar/*_subs-perc.csv": display: "subs-perc" - From 59fd55f38e82804dd36e3a7ecef24d822969c13d Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 15:36:33 +0200 Subject: [PATCH 191/226] Update conf/test_screening_count_table.config --- conf/test_screening_count_table.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_screening_count_table.config b/conf/test_screening_count_table.config index ef6b8442..52a380b9 100644 --- a/conf/test_screening_count_table.config +++ b/conf/test_screening_count_table.config @@ -23,6 +23,6 @@ params { count_table = "https://raw.githubusercontent.com/LaurenceKuhl/test-datasets/crisprseq/testdata/count_table.tsv" analysis = 'screening' mle_design_matrix = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/design_matrix.txt" - contrasts = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/rra_contrasts.txt" + contrasts = params.pipelines_testdata_base_path + "crisprseq/testdata/rra_contrasts.txt" } From fd9e43c9c69c5a710dff7b36031b525ce6834b5c Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 15:36:37 +0200 Subject: [PATCH 192/226] Update conf/test_screening_count_table.config --- conf/test_screening_count_table.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_screening_count_table.config b/conf/test_screening_count_table.config index 52a380b9..bebe8022 100644 --- a/conf/test_screening_count_table.config +++ b/conf/test_screening_count_table.config @@ -22,7 +22,7 @@ params { // Input data count_table = "https://raw.githubusercontent.com/LaurenceKuhl/test-datasets/crisprseq/testdata/count_table.tsv" analysis = 'screening' - mle_design_matrix = "https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata/design_matrix.txt" + mle_design_matrix = params.pipelines_testdata_base_path + "crisprseq/testdata/design_matrix.txt" contrasts = params.pipelines_testdata_base_path + "crisprseq/testdata/rra_contrasts.txt" } From bb48406dba5c9717e14d7f045f27a33946e881b6 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 15:37:18 +0200 Subject: [PATCH 193/226] Update conf/test_screening_count_table.config --- conf/test_screening_count_table.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_screening_count_table.config b/conf/test_screening_count_table.config index bebe8022..e80d9477 100644 --- a/conf/test_screening_count_table.config +++ b/conf/test_screening_count_table.config @@ -20,7 +20,7 @@ params { max_time = '6.h' // Input data - count_table = "https://raw.githubusercontent.com/LaurenceKuhl/test-datasets/crisprseq/testdata/count_table.tsv" + count_table = params.pipelines_testdata_base_path + "crisprseq/testdata/count_table.tsv" analysis = 'screening' mle_design_matrix = params.pipelines_testdata_base_path + "crisprseq/testdata/design_matrix.txt" contrasts = params.pipelines_testdata_base_path + "crisprseq/testdata/rra_contrasts.txt" From 7248ddae2452500b6866c5c4f2739558029e54bb Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 19:43:25 +0200 Subject: [PATCH 194/226] Update conf/test_screening_count_table.config MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Júlia Mir Pedrol --- conf/test_screening_count_table.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_screening_count_table.config b/conf/test_screening_count_table.config index e80d9477..9dfefe96 100644 --- a/conf/test_screening_count_table.config +++ b/conf/test_screening_count_table.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/crisprseq -profile test_screening, --outdir + nextflow run nf-core/crisprseq -profile test_screening_count_table, --outdir ---------------------------------------------------------------------------------------- */ From 1c88a2278e7dae6eb5c4bdbe928c8c258092e0f2 Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Mon, 27 May 2024 19:45:06 +0200 Subject: [PATCH 195/226] Update subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Júlia Mir Pedrol --- .../local/utils_nfcore_crisprseq_pipeline/main.nf | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index 08e346ef..a3e4ee74 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -107,11 +107,11 @@ workflow PIPELINE_INITIALISATION { template: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], template] } .set { ch_input } - fastqc_screening = ch_input.reads_screening - fastqc_screening = ch_input.reads_screening - reference = ch_input.reference - protospacer = ch_input.protospacer - template = ch_input.template + reads_targeted = ch_input. reads_targeted + fastqc_screening = ch_input.reads_screening + reference = ch_input.reference + protospacer = ch_input.protospacer + template = ch_input.template } else { ch_input = Channel.empty() } From fd82bb231310cace0838272fbb40c8a4795017d6 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Tue, 28 May 2024 08:17:41 +0000 Subject: [PATCH 196/226] update changelog and add contribution to readme --- CHANGELOG.md | 1 + README.md | 1 + 2 files changed, 2 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 44224ef2..7b9c212e 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -15,6 +15,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Template update to 2.13.1 ([#124](https://github.com/nf-core/crisprseq/pull/124)) - Metromap added in the docs ([#128](https://github.com/nf-core/crisprseq/pull/128)) - Added MAGeCK count table in the multiqc ([#131](https://github.com/nf-core/crisprseq/pull/131)) +- Added additional plots to Tower output ([#130](https://github.com/nf-core/crisprseq/pull/130)) ### Fixed diff --git a/README.md b/README.md index 7d5dfe4f..38bad46b 100644 --- a/README.md +++ b/README.md @@ -134,6 +134,7 @@ We thank the following people for their extensive assistance in the development - [@mashehu](https://github.com/mashehu) - [@msanvicente](https://github.com/msanvicente) - [@SusiJo](https://github.com/SusiJo) +- [@mschaffer-incyte](https://github.com/mschaffer-incyte) ## Contributions and Support From f77e5e0b71099dcef7b772d25738ddf24c9f40b2 Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Tue, 28 May 2024 11:25:55 +0200 Subject: [PATCH 197/226] fix channels containing reference and protospacer --- subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index 4929ab09..0be98ae5 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -251,13 +251,15 @@ workflow INITIALISATION_CHANNEL_CREATION_TARGETED { ch_reference = Channel.fromPath(params.reference_fasta) input_protospacer .combine(ch_reference) + .map{ meta, protospacer, reference -> [ meta, reference, protospacer ]} // Change the order of the channel .set{ reference_protospacer } } else { ch_reference = Channel.fromPath(params.reference_fasta) ch_protospacer = Channel.of(params.protospacer) - ch_input_reads + input_reads .combine(ch_reference) .combine(ch_protospacer) + .map{ meta, fastqs, reference, protospacer -> [ meta, reference, protospacer ]} // Don't add fastqs to the channel .set{ reference_protospacer } } From e39eb47ded4f6d681bf519a72e1428a4806dd7a2 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Tue, 28 May 2024 11:30:10 +0200 Subject: [PATCH 198/226] Remove if statement --- subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf | 6 +----- 1 file changed, 1 insertion(+), 5 deletions(-) diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index a3e4ee74..666188c7 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -119,16 +119,12 @@ workflow PIPELINE_INITIALISATION { // // Validate input samplesheet // - if(params.input) { - ch_input.reads_targeted + ch_input.reads_targeted .groupTuple() .map { validateInputSamplesheet(it) } .set { reads_targeted } - } else { - reads_targeted = Channel.empty() - } emit: reads_targeted From 6d228d46d6146d628570de58c5bb1f7ccc412a46 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Tue, 28 May 2024 12:15:41 +0200 Subject: [PATCH 199/226] Fix link --- docs/usage/targeted.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage/targeted.md b/docs/usage/targeted.md index d1ddad37..36e68510 100644 --- a/docs/usage/targeted.md +++ b/docs/usage/targeted.md @@ -35,7 +35,7 @@ CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz,G The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 6 columns to match those defined in the table below. -A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 3 samples, where `chr6` is single-end and has a template sequence _(this is a reduced samplesheet, please refer to the [pipeline example samplesheet](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/samplesheet_test.csv) to see the full version)_. +A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 3 samples, where `chr6` is single-end and has a template sequence _(this is a reduced samplesheet, please refer to the [pipeline example samplesheet](https://raw.githubusercontent.com/nf-core/test-datasets/crisprseq/testdata-edition/samplesheet_test_full.csv) to see the full version)_. ```console sample,fastq_1,fastq_2,reference,protospacer,template From 148010f7f959be1335b8d55663f20e9bbd144cde Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Tue, 28 May 2024 12:30:34 +0200 Subject: [PATCH 200/226] Fix the count test fail --- .../utils_nfcore_crisprseq_pipeline/main.nf | 16 +++++++++------- 1 file changed, 9 insertions(+), 7 deletions(-) diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index 666188c7..1c0ceead 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -107,7 +107,7 @@ workflow PIPELINE_INITIALISATION { template: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], template] } .set { ch_input } - reads_targeted = ch_input. reads_targeted + reads_targeted = ch_input.reads_targeted fastqc_screening = ch_input.reads_screening reference = ch_input.reference protospacer = ch_input.protospacer @@ -119,12 +119,14 @@ workflow PIPELINE_INITIALISATION { // // Validate input samplesheet // - ch_input.reads_targeted - .groupTuple() - .map { - validateInputSamplesheet(it) - } - .set { reads_targeted } + if(params.input) { + ch_input.reads_targeted + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .set { reads_targeted } + } emit: reads_targeted From bfe2142643078f9a39a0a404312ec59c8d999c6a Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Tue, 28 May 2024 14:34:00 +0200 Subject: [PATCH 201/226] Fix typos --- docs/usage/targeted.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/docs/usage/targeted.md b/docs/usage/targeted.md index 36e68510..6cb65625 100644 --- a/docs/usage/targeted.md +++ b/docs/usage/targeted.md @@ -50,8 +50,8 @@ chr6,chr6-61942198-61942498_R1.fastq.gz,,CAA...GGA,TTTTATGATATTTATCTTTT,TTC...CA | `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | | `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". (Optional) | | `reference` | Reference sequence of the target region. | -| `protospacer` | Sequence of the protospacer used for CRISPR editing. Must not includ the PAM. | -| `template` | Sequence of the template used in templet-based editing experiments. (Optional) | +| `protospacer` | Sequence of the protospacer used for CRISPR editing. Must not include the PAM. | +| `template` | Sequence of the template used in template-based editing experiments. (Optional) | An [example samplesheet](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/samplesheet_test.csv) has been provided with the pipeline. From 387fb9bcf95a400d145b6ff2c56cab3798ba82c5 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Tue, 28 May 2024 15:01:22 +0200 Subject: [PATCH 202/226] Ran pre commit --- docs/usage/targeted.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/docs/usage/targeted.md b/docs/usage/targeted.md index 6cb65625..fbbbfa35 100644 --- a/docs/usage/targeted.md +++ b/docs/usage/targeted.md @@ -50,8 +50,8 @@ chr6,chr6-61942198-61942498_R1.fastq.gz,,CAA...GGA,TTTTATGATATTTATCTTTT,TTC...CA | `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | | `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". (Optional) | | `reference` | Reference sequence of the target region. | -| `protospacer` | Sequence of the protospacer used for CRISPR editing. Must not include the PAM. | -| `template` | Sequence of the template used in template-based editing experiments. (Optional) | +| `protospacer` | Sequence of the protospacer used for CRISPR editing. Must not include the PAM. | +| `template` | Sequence of the template used in template-based editing experiments. (Optional) | An [example samplesheet](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/samplesheet_test.csv) has been provided with the pipeline. From e7935bd32a63a7a6aad9553d0385e443f11b8b52 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Tue, 28 May 2024 15:12:28 +0200 Subject: [PATCH 203/226] Update download pipeline --- .github/workflows/download_pipeline.yml | 9 ++++++--- 1 file changed, 6 insertions(+), 3 deletions(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 627d9c76..2d20d644 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -30,11 +30,14 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: "3.11" + python-version: "3.12" architecture: "x64" - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: From d0db3abf86794ca5e4f0bcb571dfe0ea90e942e3 Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Tue, 28 May 2024 16:13:16 +0200 Subject: [PATCH 204/226] change single_end from the channels if PEAR was used --- workflows/crisprseq_targeted.nf | 100 ++++++++++++++++++++++++++------ 1 file changed, 82 insertions(+), 18 deletions(-) diff --git a/workflows/crisprseq_targeted.nf b/workflows/crisprseq_targeted.nf index 3e0a02dc..46a2d563 100644 --- a/workflows/crisprseq_targeted.nf +++ b/workflows/crisprseq_targeted.nf @@ -157,6 +157,47 @@ workflow CRISPRSEQ_TARGETED { .set { ch_pear_fastq } ch_versions = ch_versions.mix(PEAR.out.versions) + // Change reference, protospacer and template channels to have the same meta information as the reads + ch_pear_fastq + .map {meta, reads -> + single_end = meta.single_end + return [ meta - meta.subMap('single_end'), reads, single_end ] + } + .tap { no_single_end } + .join( + ORIENT_REFERENCE.out.reference + .map {meta, reference -> + return [ meta - meta.subMap('single_end'), reference ] + } + ) + .map {meta, reads, single_end, reference -> + return [ meta + ["single_end": single_end], reference ] + } + .tap{ ch_oriented_reference } + no_single_end + .join( + INITIALISATION_CHANNEL_CREATION_TARGETED.out.template + .map {meta, template -> + return [ meta - meta.subMap('single_end'), template ] + } + ) + .map {meta, reads, single_end, template -> + return [ meta + ["single_end": single_end], template ] + } + .set{ ch_template } + no_single_end + .join( + INITIALISATION_CHANNEL_CREATION_TARGETED.out.reference_protospacer + .map {meta, reference, protospacer -> + return [ meta - meta.subMap('single_end'), protospacer ] + } + ) + .map {meta, reads, single_end, protospacer -> + return [ meta + ["single_end": single_end], protospacer ] + } + .set{ ch_protospacer } + + // // MODULE: Run FastQC // @@ -223,25 +264,48 @@ workflow CRISPRSEQ_TARGETED { ch_cat_fastq.paired .mix(ch_cat_fastq.single) .join(PEAR.out.assembled, remainder: true) - .join(SEQTK_SEQ_MASK.out.fastx) - .join(CUTADAPT.out.log) - .map { meta, reads, assembled, masked, trimmed -> + .map { meta, reads, assembled -> + return [ meta - meta.subMap('single_end'), reads, assembled] + } + .join( + SEQTK_SEQ_MASK.out.fastx + .map { meta, masked -> + single_end = meta.single_end + return [ meta - meta.subMap('single_end'), masked, single_end] + } + ) + .join( + CUTADAPT.out.log + .map { meta, trimmed -> + return [ meta - meta.subMap('single_end'), trimmed] + } + ) + .map { meta, reads, assembled, masked, single_end, trimmed -> if (assembled == null) { assembled = [] } - return [ meta, reads, assembled, masked, trimmed ] + return [ meta + ["single_end": single_end], reads, assembled, masked, trimmed ] } .set { ch_preprocessing_summary_data } } else { ch_cat_fastq.paired .mix(ch_cat_fastq.single) .join(PEAR.out.assembled, remainder: true) - .join(SEQTK_SEQ_MASK.out.fastx) - .map { meta, reads, assembled, masked -> + .map { meta, reads, assembled -> + return [ meta - meta.subMap('single_end'), reads, assembled] + } + .join( + SEQTK_SEQ_MASK.out.fastx + .map { meta, masked -> + single_end = meta.single_end + return [ meta - meta.subMap('single_end'), masked, single_end] + } + ) + .map { meta, reads, assembled, masked, single_end -> if (assembled == null) { assembled = [] } - return [ meta, reads, assembled, masked, [] ] + return [ meta + ["single_end": single_end], reads, assembled, masked, [] ] } .set { ch_preprocessing_summary_data } } @@ -494,7 +558,7 @@ workflow CRISPRSEQ_TARGETED { if (params.aligner == "minimap2") { MINIMAP2_ALIGN_ORIGINAL ( ch_preprocess_reads - .join(ORIENT_REFERENCE.out.reference), + .join(ch_oriented_reference), true, false, true @@ -508,7 +572,7 @@ workflow CRISPRSEQ_TARGETED { // if (params.aligner == "bwa") { BWA_INDEX ( - ORIENT_REFERENCE.out.reference + ch_oriented_reference ) ch_versions = ch_versions.mix(BWA_INDEX.out.versions) BWA_MEM ( @@ -525,7 +589,7 @@ workflow CRISPRSEQ_TARGETED { // if (params.aligner == "bowtie2") { BOWTIE2_BUILD ( - ORIENT_REFERENCE.out.reference + ch_oriented_reference ) ch_versions = ch_versions.mix(BOWTIE2_BUILD.out.versions) BOWTIE2_ALIGN ( @@ -561,8 +625,8 @@ workflow CRISPRSEQ_TARGETED { // MODULE: Obtain a new reference with the template modification // TEMPLATE_REFERENCE ( - ORIENT_REFERENCE.out.reference - .join(INITIALISATION_CHANNEL_CREATION_TARGETED.out.template) + ch_oriented_reference + .join(ch_template) ) ch_versions = ch_versions.mix(TEMPLATE_REFERENCE.out.versions.first()) @@ -572,7 +636,7 @@ workflow CRISPRSEQ_TARGETED { // MINIMAP2_ALIGN_TEMPLATE ( TEMPLATE_REFERENCE.out.fasta - .join(ORIENT_REFERENCE.out.reference), + .join(ch_oriented_reference), true, false, true @@ -583,9 +647,9 @@ workflow CRISPRSEQ_TARGETED { ch_mapped_bam .join(SAMTOOLS_INDEX.out.bai) - .join(ORIENT_REFERENCE.out.reference) - .join(ch_input_protospacer) - .join(INITIALISATION_CHANNEL_CREATION_TARGETED.out.template, remainder: true) + .join(ch_oriented_reference) + .join(ch_protospacer) + .join(ch_template, remainder: true) .join(ch_template_bam, remainder: true) .join(TEMPLATE_REFERENCE.out.fasta, remainder: true) .join(ALIGNMENT_SUMMARY.out.summary) @@ -621,8 +685,8 @@ workflow CRISPRSEQ_TARGETED { // CRISPRSEQ_PLOTTER ( CIGAR_PARSER.out.indels - .join(ORIENT_REFERENCE.out.reference) - .join(ch_input_protospacer) + .join(ch_oriented_reference) + .join(ch_protospacer) ) ch_versions = ch_versions.mix(CRISPRSEQ_PLOTTER.out.versions.first()) From 93f5d2c25911b74644aad7178e74b0c856edb5d3 Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Tue, 28 May 2024 16:37:43 +0200 Subject: [PATCH 205/226] add pattern for protospacer validation --- nextflow_schema.json | 2 ++ 1 file changed, 2 insertions(+) diff --git a/nextflow_schema.json b/nextflow_schema.json index f2034403..c1943f3d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -104,6 +104,8 @@ }, "protospacer": { "type": "string", + "pattern": "^[ACGTacgt]+$", + "errorMessage": "The protospacer must be a valid DNA sequence.", "fa_icon": "fas fa-grip-lines", "description": "Provide the same protospacer sequence for all samples. Will override protospacer sequences provided by an input samplesheet." } From 0b39e5be3dc6ad906ed04045337ae5e0249f534b Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Tue, 28 May 2024 16:37:53 +0200 Subject: [PATCH 206/226] update changelog --- CHANGELOG.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 44224ef2..ce527a64 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -19,7 +19,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Fixed - Adapt cutadapt module to work with single-end and paired-end reads again ([#121](https://github.com/nf-core/crisprseq/pull/121)) +- Fix premature completion of the pipeline when paired-end reads were merged ([#145](https://github.com/nf-core/crisprseq/pull/145)) - Create empty \*-QC-indels.csv file if alignments not found. ([#138](https://github.com/nf-core/crisprseq/pull/138)) +- Fix `--reference_fasta` and `--protospacer` parameters ([#144](https://github.com/nf-core/crisprseq/pull/144)) ## [v2.1.1 - Jamon Salas - patch](https://github.com/nf-core/crisprseq/releases/tag/2.1.1) - [14.12.2023] From af1cbe46a336a90b1542ae2ba475fb10e86d4a4f Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Tue, 28 May 2024 16:07:51 +0000 Subject: [PATCH 207/226] move validation of input channels to the main if block --- .../utils_nfcore_crisprseq_pipeline/main.nf | 24 +++++++++---------- 1 file changed, 11 insertions(+), 13 deletions(-) diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index cefb5168..a2a5a95f 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -107,25 +107,23 @@ workflow PIPELINE_INITIALISATION { template: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], template] } .set { ch_input } - reads_targeted = ch_input.reads_targeted - fastqc_screening = ch_input.reads_screening - reference = ch_input.reference - protospacer = ch_input.protospacer - template = ch_input.template - } else { - ch_input = Channel.empty() - } - // - // Validate input samplesheet - // - if(params.input) { + // + // Validate input samplesheet + // ch_input.reads_targeted .groupTuple() .map { validateInputSamplesheet(it) } .set { reads_targeted } + + fastqc_screening = ch_input.reads_screening + reference = ch_input.reference + protospacer = ch_input.protospacer + template = ch_input.template + } else { + ch_input = Channel.empty() } emit: @@ -134,7 +132,7 @@ workflow PIPELINE_INITIALISATION { reference protospacer template - versions = ch_versions + versions = ch_versions } /* From 081ca39a0ffc21db0ab0017b2e8e1f1993d354ba Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?J=C3=BAlia=20Mir=20Pedrol?= Date: Wed, 29 May 2024 10:30:46 +0200 Subject: [PATCH 208/226] Apply suggestions from code review --- workflows/crisprseq_targeted.nf | 5 +++++ 1 file changed, 5 insertions(+) diff --git a/workflows/crisprseq_targeted.nf b/workflows/crisprseq_targeted.nf index 46a2d563..857867c7 100644 --- a/workflows/crisprseq_targeted.nf +++ b/workflows/crisprseq_targeted.nf @@ -160,6 +160,7 @@ workflow CRISPRSEQ_TARGETED { // Change reference, protospacer and template channels to have the same meta information as the reads ch_pear_fastq .map {meta, reads -> + // save single_end value and remove the key from the meta map single_end = meta.single_end return [ meta - meta.subMap('single_end'), reads, single_end ] } @@ -167,10 +168,12 @@ workflow CRISPRSEQ_TARGETED { .join( ORIENT_REFERENCE.out.reference .map {meta, reference -> + // Remove single_end from the meta map to allow joining two channels with different single_end values return [ meta - meta.subMap('single_end'), reference ] } ) .map {meta, reads, single_end, reference -> + // Add the correct single_end value to the reference meta map. return [ meta + ["single_end": single_end], reference ] } .tap{ ch_oriented_reference } @@ -265,6 +268,7 @@ workflow CRISPRSEQ_TARGETED { .mix(ch_cat_fastq.single) .join(PEAR.out.assembled, remainder: true) .map { meta, reads, assembled -> + // Remove the single_end key from the meta map to allow joining channels with different single_end values return [ meta - meta.subMap('single_end'), reads, assembled] } .join( @@ -292,6 +296,7 @@ workflow CRISPRSEQ_TARGETED { .mix(ch_cat_fastq.single) .join(PEAR.out.assembled, remainder: true) .map { meta, reads, assembled -> + // Remove the single_end key from the meta map to allow joining channels with different single_end values return [ meta - meta.subMap('single_end'), reads, assembled] } .join( From 6820537bfb9f6041243f447af87c6833846a0800 Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Fri, 31 May 2024 11:05:21 +0200 Subject: [PATCH 209/226] fix bug accessing undefined variable index_filters --- bin/cigar_parser.R | 1 - 1 file changed, 1 deletion(-) diff --git a/bin/cigar_parser.R b/bin/cigar_parser.R index cace0d1c..1cfc04df 100755 --- a/bin/cigar_parser.R +++ b/bin/cigar_parser.R @@ -1234,7 +1234,6 @@ if (dim(alignment_info)[1] != 0){ colnames(edit_summary_perc)[1] = results_path # Rename the column to add the sample ID edit_summary_perc <- t(edit_summary_perc) # t() will add classes as columns and counts as values, 1 row per sample write.csv(edit_summary_perc,file=paste0(results_path, "_edits.csv")) - indel_filters prevc_classes_mqc <- c("Wt passing filter", "Wt NOT passing filter", "Indels NOT passing filter", "Above error & in pick", "NOT above error & in pick", "NOT above error & NOT in pick", "Above error & NOT in pick") prevc_counts_mqc <- c(0, 0, 0, 0, 0, 0, 0) From 930a09ca96753dadc623ce161ff9665dd5d62914 Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Mon, 3 Jun 2024 11:24:03 +0200 Subject: [PATCH 210/226] write empty reads-summary.csv file --- bin/cigar_parser.R | 3 +++ 1 file changed, 3 insertions(+) diff --git a/bin/cigar_parser.R b/bin/cigar_parser.R index 1cfc04df..f13e521c 100755 --- a/bin/cigar_parser.R +++ b/bin/cigar_parser.R @@ -1219,6 +1219,9 @@ if (dim(alignment_info)[1] != 0){ htmlwidgets::saveWidget(plotly::as_widget(fig), paste0(results_path,"_edition.html")) }else{ + reads_classes <- c("Raw reads", "Merged reads", "Quality filtered reads", "Clustered reads", "Aligned reads") + reads_summary <- data.frame(classes = unlist(reads_classes), counts = unlist(reads_counts)) + write.csv(reads_summary,file=paste0(results_path, "_reads-summary.csv")) fig<-empty_plot("No alignments were produced. Please check your files and references") htmlwidgets::saveWidget(plotly::as_widget(fig), paste0(results_path,"_edition.html")) From 9b0b2a71683f7a844b46a683fb2fcd5f50b9dd41 Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Tue, 4 Jun 2024 09:50:47 +0200 Subject: [PATCH 211/226] add empty reads_counts --- bin/cigar_parser.R | 1 + 1 file changed, 1 insertion(+) diff --git a/bin/cigar_parser.R b/bin/cigar_parser.R index f13e521c..2092a926 100755 --- a/bin/cigar_parser.R +++ b/bin/cigar_parser.R @@ -1220,6 +1220,7 @@ if (dim(alignment_info)[1] != 0){ }else{ reads_classes <- c("Raw reads", "Merged reads", "Quality filtered reads", "Clustered reads", "Aligned reads") + reads_counts <- c(0, 0, 0, 0, 0) reads_summary <- data.frame(classes = unlist(reads_classes), counts = unlist(reads_counts)) write.csv(reads_summary,file=paste0(results_path, "_reads-summary.csv")) fig<-empty_plot("No alignments were produced. From d38935f98ec41bbfc41c63d4642e0e7dced508fb Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Wed, 5 Jun 2024 11:31:32 +0200 Subject: [PATCH 212/226] fix prlotter, pattern doesn't exist --- bin/plotter.R | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/bin/plotter.R b/bin/plotter.R index 8d0fd8ea..76d7f821 100755 --- a/bin/plotter.R +++ b/bin/plotter.R @@ -553,7 +553,7 @@ if (dim(data)[2]>3 && length(checkFaulty) == 0 && length(checkEmpty) == 0){ ### templata_based <- data$t_reads[1] ### 0 total_char <- wt + templata_based + dim(data)[1] - delCols_indels <- data %>% group_by(Modification, Start, Length, ins_nt, patterns) %>% dplyr::summarize(freq = n()) + delCols_indels <- data %>% group_by(Modification, Start, Length, ins_nt) %>% dplyr::summarize(freq = n()) unique_variants <- rbind(as.data.frame(delCols_indels), c("wt", 0, 0, NA, NA, wt), c("template-based", 0, 0, NA, NA, templata_based)) uniq_indels_sorted <- unique_variants[order(as.numeric(unique_variants$freq), decreasing = TRUE),] write.csv(uniq_indels_sorted,file=paste0(sample_name, "_unique-variants.csv")) From 3d5cc1d2d438c229d570771731acd0c86f9da2da Mon Sep 17 00:00:00 2001 From: LaurenceKuhl Date: Thu, 6 Jun 2024 11:42:10 +0200 Subject: [PATCH 213/226] Update test_screening_paired.config --- conf/test_screening_paired.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_screening_paired.config b/conf/test_screening_paired.config index 7ff0a45d..1115f2a6 100644 --- a/conf/test_screening_paired.config +++ b/conf/test_screening_paired.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/crisprseq -profile test_screening, --outdir + nextflow run nf-core/crisprseq -profile test_screening_paired, --outdir ---------------------------------------------------------------------------------------- */ From 24486e10982d3b7b6dfea18f5b792e31b3f5ae74 Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Thu, 6 Jun 2024 17:25:12 +0200 Subject: [PATCH 214/226] avoid converting T nucleotides to TRUE --- bin/plotter.R | 9 ++++++++- 1 file changed, 8 insertions(+), 1 deletion(-) diff --git a/bin/plotter.R b/bin/plotter.R index 76d7f821..0cce6b1f 100755 --- a/bin/plotter.R +++ b/bin/plotter.R @@ -54,10 +54,17 @@ gR <- opt$gRNA_sequence substitutions_info <- opt$substitutions_info rel_cut_site <- as.numeric(opt$cut_site) -data <- read.csv(indels_info) +data <- read.csv(indels_info, colClasses = c("character")) ref_seq <- readFasta(reference) subs_plup <- read.csv(substitutions_info, row.names = 1) +data$Start <- as.numeric(data$Start) +data$Length <- as.numeric(data$Length) +data$freq <- as.numeric(data$freq) +data$Perc <- as.numeric(data$Perc) +data$cut_site <- as.numeric(data$cut_site) +data$wt_reads <- as.numeric(data$wt_reads) +data$t_reads <- as.numeric(data$t_reads) ###################### ##### CRISPR-GA-1 like plot https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184265/ From 06ca4b5b9bd94d1ce97a9799100feb470e6cf203 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Mon, 10 Jun 2024 14:20:43 +0200 Subject: [PATCH 215/226] Fix typos --- assets/multiqc_config.yml | 4 ++-- modules/local/mageck/flutemle.nf | 10 +++++----- 2 files changed, 7 insertions(+), 7 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 11624a0b..8ddc9dd0 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -184,11 +184,11 @@ custom_data: format: "{:,.0f}" Zerocounts: title: "Total number of missing sgRNAs" - description: "sgRNAs that have 0 counts, recommended: no more than 1%)" + description: "sgRNAs that have 0 counts, recommended: no more than 1%" format: "{:,.0f}" GiniIndex: title: "Gini Index" - description: "Measure of statistical dispersion. A smaller value indicates more eveness of the count distribution." + description: "Measure of statistical dispersion. A smaller value indicates more evenness of the count distribution." max: 1 min: 0 diff --git a/modules/local/mageck/flutemle.nf b/modules/local/mageck/flutemle.nf index 5ee890fd..e9efbaaa 100644 --- a/modules/local/mageck/flutemle.nf +++ b/modules/local/mageck/flutemle.nf @@ -12,11 +12,11 @@ process MAGECK_FLUTEMLE { tuple val(meta), path(gene_summary) output: - tuple val(meta), path("MAGeCKFlute_*/Enrichment/*"), emit: enrich - tuple val(meta), path("MAGeCKFlute_*/QC/*"), emit: qc - tuple val(meta), path("MAGeCKFlute_*/Selection/*"), emit: select - tuple val(meta), path("MAGeCKFlute_*/PathwayView/*"), emit: pathwayview - path "versions.yml" , emit: versions + tuple val(meta), path("MAGeCKFlute_*/Enrichment/*") , emit: enrich + tuple val(meta), path("MAGeCKFlute_*/QC/*") , emit: qc + tuple val(meta), path("MAGeCKFlute_*/Selection/*") , emit: select + tuple val(meta), path("MAGeCKFlute_*/PathwayView/*"), emit: pathwayview + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when From 1f2cb2dd734c63c96c4e50e82524d3e2166ca379 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Mon, 10 Jun 2024 14:39:27 +0200 Subject: [PATCH 216/226] Fix typos --- modules/local/bagel2/pr.nf | 4 ++-- .../local/utils_nfcore_crisprseq_pipeline/main.nf | 8 +++----- 2 files changed, 5 insertions(+), 7 deletions(-) diff --git a/modules/local/bagel2/pr.nf b/modules/local/bagel2/pr.nf index 7b3c4955..5a8faeff 100644 --- a/modules/local/bagel2/pr.nf +++ b/modules/local/bagel2/pr.nf @@ -12,8 +12,8 @@ process BAGEL2_PR { tuple val(meta), path(bf), path(reference_essentials), path(reference_nonessentials) output: - tuple val(meta), path("*.tsv") , emit: pr - path "versions.yml" , emit: versions + tuple val(meta), path("*.tsv"), emit: pr + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index a2a5a95f..62a06dc6 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -77,8 +77,6 @@ workflow PIPELINE_INITIALISATION { // validateInputParameters() - - reads_targeted = Channel.empty() reads_screening = Channel.empty() fastqc_screening = Channel.empty() @@ -172,9 +170,9 @@ workflow INITIALISATION_CHANNEL_CREATION_SCREENING { emit: - library = ch_library // channel: library file - crisprcleanr = ch_crisprcleanr // channel: crisprcleanr file or value - design = ch_design // channel: design matrix file + library = ch_library // channel: library file + crisprcleanr = ch_crisprcleanr // channel: crisprcleanr file or value + design = ch_design // channel: design matrix file } /* From e0cb0d25199f7bee8628bfc6c13334c6274f015c Mon Sep 17 00:00:00 2001 From: mirpedrol Date: Fri, 14 Jun 2024 10:13:00 +0200 Subject: [PATCH 217/226] add suggestions from code review --- .nf-core.yml | 1 - docs/usage/screening.md | 14 +++++++------- main.nf | 8 ++++---- nextflow_schema.json | 2 +- .../local/utils_nfcore_crisprseq_pipeline/main.nf | 8 ++++---- 5 files changed, 16 insertions(+), 17 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index 3d6cbd9f..edba5fb7 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -5,6 +5,5 @@ lint: - conf/test.config - conf/test_full.config files_unchanged: - - lib/NfcoreTemplate.groovy # Introduced a change ahead of the nf-core/tools release - .github/PULL_REQUEST_TEMPLATE.md nf_core_version: "2.14.1" diff --git a/docs/usage/screening.md b/docs/usage/screening.md index 4d375b64..cc7f3be7 100644 --- a/docs/usage/screening.md +++ b/docs/usage/screening.md @@ -48,23 +48,23 @@ MAGeCK count which is the main alignment software used is normally able to autom ### bowtie2 -The MAGeCK count module supports bam files, which allows you to align with bowtie2 first. If you wish to do so (for instance to allow library with mismatches or to set the aligner with specific flags) you can provide a fasta file with `--fasta`. Currently, you also still need to provide the library file. +The MAGeCK count module supports bam files, which allows you to align with bowtie2 first. If you wish to do so (for instance to allow mapping reads to the library with mismatches or to set the aligner with specific flags) you can provide a fasta file with `--fasta` encoding the library. Currently, you also still need to provide the tab-separated library file with `--library`. ### library If you are running the pipeline with fastq files and wish to obtain a count table, the library parameter is needed. The library table has three mandatory columns : id, target transcript (or gRNA sequence) and gene symbol. An [example](https://github.com/nf-core/test-datasets/blob/crisprseq/testdata/brunello_target_sequence.txt) has been provided with the pipeline. Many libraries can be found on [addgene](https://www.addgene.org/). -After the alignment step, if you are performing KO (Knock-Out) screens, you can choose to correction of gene independent cell responses to CRISPR-cas9 targeting using crisprcleanr. If you are performing a CRISPR interference or activation screen, this step is not needed. +After the alignment step, if you are performing KO (Knock-Out) screens, you can choose to correct gene-independent cell responses to CRISPR-Cas9 targeting using CRISPRcleanR. If you are performing a CRISPR interference or activation screen, this step is not needed. -The pipeline currently supports 3 algorithms to detect gene essentiality, MAGeCK rra, MAGeCK mle and BAGEL2. MAGeCK MLE (Maximum Likelihood Estimation) and MAGeCK RRA (Robust Ranking Aggregation) are two different methods provided by the MAGeCK software package to analyze CRISPR-Cas9 screens. BAGEL2 identifies gene essentiality through Bayesian Analysis. +The pipeline currently supports 3 algorithms to detect gene essentiality, MAGeCK RRA, MAGeCK MLE and BAGEL2. MAGeCK MLE (Maximum Likelihood Estimation) and MAGeCK RRA (Robust Ranking Aggregation) are two different methods provided by the MAGeCK software package to analyze CRISPR-Cas9 screens. BAGEL2 identifies gene essentiality through Bayesian Analysis. We recommend to run MAGeCK MLE and BAGEL2 as these are the most used and most recent algorithms to determine gene essentiality. ### Running CRISPRcleanR -CRISPRcleanR is used for gene count normalization and the removal of biases for genomic segments for which copy numbers are amplified. Currently, the pipeline supports annotation libraries already present in the R package or a annotation file the user can provide. +[CRISPRcleanR](https://github.com/francescojm/CRISPRcleanR) is used for gene count normalization and the removal of biases for genomic segments for which copy numbers are amplified. Currently, the pipeline supports annotation libraries already present in the R package or user-provided annotation files. Most used library already have an annotation dataset which you can find [here](https://github.com/francescojm/CRISPRcleanR/blob/master/Reference_Manual.pdf). To use CRISPRcleanR normalization, use `--crisprcleanr library`, `library` being the exact name as the library in the CRISPRcleanR documentation (e.g: "AVANA_Library"). -Otherwise, if you wish to provide your own file, please provide it in csv form, and make sure it follows the following format, with the comma in front of "CODE" included : +Otherwise, if you wish to provide your own file, please provide it in CSV format, and make sure it follows the following format (with the comma in front of "CODE" included): | ,CODE | GENES | EXONE | CHRM | STRAND | STARTpos | ENDpos | | -------------------- | ----------- | ------------- | ---- | ------ | -------- | -------- | @@ -89,7 +89,7 @@ Running MAGeCK MLE and BAGEL2 with a contrast file will also output a Venn diagr ### Running MAGeCK RRA only -MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK rra, you can define the contrasts as previously stated in the last section (with a `.txt` extension) and also specify `--rra` . +MAGeCK RRA performs robust ranking aggregation to identify genes that are consistently ranked highly across multiple replicate screens. To run MAGeCK RRA, you can define the contrasts as previously stated in the last section (with a `.txt` extension) and also specify `--rra`. ### Running MAGeCK MLE only @@ -112,7 +112,7 @@ BAGEL2 uses the same contrasts from `--contrasts`. ### MAGECKFlute -The downstream analysis involves distinguishing essential, non-essential, and target-associated genes. Additionally, it encompasses conducting biological functional category analysis and pathway enrichment analysis for these genes. Furthermore, the function provides visualization of genes within pathways, enhancing user exploration of screening data. MAGECKFlute is run automatically after MAGeCK MLE and for each MLE design matrice. If you have used the `--day0_label`, MAGeCKFlute will be ran on all the other conditions. Please note that the DepMap data is used for these plots. +The downstream analysis involves distinguishing essential, non-essential, and target-associated genes. Additionally, it encompasses conducting biological functional category analysis and pathway enrichment analysis for these genes. Furthermore, it provides visualization of genes within pathways, enhancing user exploration of screening data. MAGECKFlute is run automatically after MAGeCK MLE and for each MLE design matrice. If you have used the `--day0_label`, MAGeCKFlute will be ran on all the other conditions. Please note that the DepMap data is used for these plots. Note that the pipeline will create the following files in your working directory: diff --git a/main.nf b/main.nf index f1a43efe..e06415e3 100644 --- a/main.nf +++ b/main.nf @@ -44,11 +44,11 @@ include { CRISPRSEQ_SCREENING } from './workflows/crisprseq_screening' workflow NFCORE_CRISPRSEQ { take: - reads_targeted // channel: fastqc files read in from --input + reads_targeted // channel: fastqc files read in from --input reads_screening // channel: fastqc files read in from --input - reference // channel: reference sequence read from --input - protospacer // channel: protospacer sequence read from --input - template // channel: template sequence read from --input + reference // channel: reference sequence read from --input + protospacer // channel: protospacer sequence read from --input + template // channel: template sequence read from --input main: // diff --git a/nextflow_schema.json b/nextflow_schema.json index d183ac67..dc4fe674 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -171,7 +171,7 @@ }, "fasta": { "type": "string", - "description": "Fasta file in case you want to map with bowtie2 and then MAGeCK count" + "description": "Library in fasta file format in case you want to map with bowtie2 and then MAGeCK count" }, "day0_label": { "type": "string", diff --git a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf index 62a06dc6..1a73efd1 100644 --- a/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_crisprseq_pipeline/main.nf @@ -98,11 +98,11 @@ workflow PIPELINE_INITIALISATION { } else { files = [ fastq_1 ] } - reads_targeted: [ meta.id, meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], files ] + reads_targeted: [ meta.id, meta - meta.subMap('condition') + [ single_end : fastq_2 ? false : true, self_reference : reference ? false : true, template : template ? true : false ], files ] reads_screening:[ meta + [ single_end:fastq_2?false:true ], files ] - reference: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], reference] - protospacer: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], protospacer] - template: [meta - meta.subMap('condition') + [ single_end:fastq_2?false:true, self_reference:reference?false:true, template:template?true:false ], template] + reference: [meta - meta.subMap('condition') + [ single_end : fastq_2 ? false : true, self_reference : reference ? false : true, template : template ? true : false ], reference] + protospacer: [meta - meta.subMap('condition') + [ single_end : fastq_2 ? false : true, self_reference : reference ? false : true, template : template ? true : false ], protospacer] + template: [meta - meta.subMap('condition') + [ single_end : fastq_2 ? false : true, self_reference : reference ? false : true, template : template ? true : false ], template] } .set { ch_input } From e3560322a5e2a97385df9594b581df5b98c0fbd7 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 19 Jun 2024 11:01:51 +0200 Subject: [PATCH 218/226] Updating mageck count --- modules.json | 88 +++++++++++---- modules/nf-core/mageck/count/environment.yml | 2 +- .../nf-core/mageck/count/mageck-count.diff | 42 +++++--- modules/nf-core/mageck/count/main.nf | 9 +- modules/nf-core/mageck/count/meta.yml | 4 +- .../nf-core/mageck/count/tests/main.nf.test | 60 +++++++++++ .../mageck/count/tests/main.nf.test.snap | 100 ++++++++++++++++++ modules/nf-core/mageck/count/tests/tags.yml | 2 + 8 files changed, 263 insertions(+), 44 deletions(-) create mode 100644 modules/nf-core/mageck/count/tests/main.nf.test create mode 100644 modules/nf-core/mageck/count/tests/main.nf.test.snap create mode 100644 modules/nf-core/mageck/count/tests/tags.yml diff --git a/modules.json b/modules.json index 3e576767..b2b81fa2 100644 --- a/modules.json +++ b/modules.json @@ -8,115 +8,157 @@ "bowtie2/align": { "branch": "master", "git_sha": "0fe30831abbc2ed115e46e92330edf38f56edc3d", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bowtie2/build": { "branch": "master", "git_sha": "1fea64f5132a813ec97c1c6d3a74e0aee7142b6d", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/index": { "branch": "master", "git_sha": "086fa66260595e123b0ea47a6512539b72a9afa3", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/mem": { "branch": "master", "git_sha": "0c34b8159f62cde451c4ff249629c9d0a4f3f9c3", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cat/fastq": { "branch": "master", "git_sha": "4fc983ad0b30e6e32696fa7d980c76c7bfe1c03e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" }, "cutadapt": { "branch": "master", "git_sha": "6618151ed69274863dc6fe6d2920afa90abaca1f", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/cutadapt/cutadapt.diff" }, "fastqc": { "branch": "master", "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "mageck/count": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "git_sha": "3fd600f14c03766f840acf29e1a6a1bc45d5c9a0", + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/count/mageck-count.diff" }, "mageck/mle": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" }, "mageck/test": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/test/mageck-test.diff" }, "medaka": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/medaka/medaka.diff" }, "minimap2/align": { "branch": "master", "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" }, "minimap2/index": { "branch": "master", "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "pear": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "racon": { "branch": "master", "git_sha": "f5ed3ac0834b68e80a00a06a61d04ce8e896f275", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/index": { "branch": "master", "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "seqtk/seq": { "branch": "master", "git_sha": "7f88aae93c69586c0789322b77743ee0ef469502", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "vsearch/cluster": { "branch": "master", "git_sha": "d97b335eb448073c1b680710303c02a55f40c77c", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" }, "vsearch/sort": { "branch": "master", "git_sha": "97cf4c4a37a8931e19ef728de92f930a366669f2", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] } } }, @@ -125,4 +167,4 @@ } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/mageck/count/environment.yml b/modules/nf-core/mageck/count/environment.yml index 0729b284..c7221d6f 100644 --- a/modules/nf-core/mageck/count/environment.yml +++ b/modules/nf-core/mageck/count/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::mageck=0.5.9 + - bioconda::mageck=0.5.9.5 diff --git a/modules/nf-core/mageck/count/mageck-count.diff b/modules/nf-core/mageck/count/mageck-count.diff index 541f87cc..a7a7d72d 100644 --- a/modules/nf-core/mageck/count/mageck-count.diff +++ b/modules/nf-core/mageck/count/mageck-count.diff @@ -1,16 +1,17 @@ Changes in module 'nf-core/mageck/count' --- modules/nf-core/mageck/count/main.nf +++ modules/nf-core/mageck/count/main.nf -@@ -1,6 +1,6 @@ +@@ -1,19 +1,22 @@ process MAGECK_COUNT { tag "$meta.id" - label 'process_medium' + label 'process_high' - conda "${moduleDir}/environment.yml" +- conda "${moduleDir}/environment.yml" ++ container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? -@@ -8,13 +8,16 @@ - 'biocontainers/mageck:0.5.9--py37h6bb024c_0' }" + 'https://depot.galaxyproject.org/singularity/mageck:0.5.9.5--py39h1f90b4d_3': + 'biocontainers/mageck:0.5.9.5--py39h1f90b4d_3' }" input: - tuple val(meta), path(inputfile) @@ -18,27 +19,24 @@ Changes in module 'nf-core/mageck/count' path(library) output: -- tuple val(meta), path("*count*.txt"), emit: count +- tuple val(meta), path("*count.txt") , emit: count + tuple val(meta), path("*count.txt") , emit: count tuple val(meta), path("*.count_normalized.txt"), emit: norm -- path "versions.yml" , emit: versions + tuple val(meta), path("*.countsummary.txt") , emit: summary + tuple val(meta), path("*.count_normalized.txt"), emit: normalized + tuple val(meta), path("*.log") , emit: logs -+ path "versions.yml" , emit: versions + path "versions.yml" , emit: versions when: - task.ext.when == null || task.ext.when -@@ -22,8 +25,14 @@ +@@ -22,8 +25,13 @@ script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def input_file = ("$inputfile".endsWith(".fastq.gz")) ? "--fastq ${inputfile}" : "-k ${inputfile}" - def sample_label = ("$inputfile".endsWith(".fastq.gz") || "$inputfile".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' -+ // def input_file = ("$inputfile".endsWith(".fastq.gz") || "$inputfile".endsWith(".fq.gz")) ? "--fastq ${inputfile}" : "-k ${inputfile}" + def sample_label = ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' + -+ if ((meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) || "$fastq1".endsWith(".bam"))) { ++ if (meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) || "$fastq1".endsWith(".bam")) { + input = "--fastq $fastq1" + } else { + input = "--fastq $fastq1 --fastq-2 $fastq2" @@ -46,7 +44,7 @@ Changes in module 'nf-core/mageck/count' """ mageck \\ -@@ -32,7 +41,7 @@ +@@ -32,7 +40,7 @@ -l $library \\ -n $prefix \\ $sample_label \\ @@ -55,5 +53,23 @@ Changes in module 'nf-core/mageck/count' cat <<-END_VERSIONS > versions.yml - +@@ -40,17 +48,4 @@ + mageck: \$(mageck -v) + END_VERSIONS + """ +- stub: +- def args = task.ext.args ?: '' +- def prefix = task.ext.prefix ?: "${meta.id}" +- def input_file = ("$inputfile".endsWith(".fastq.gz")) ? "--fastq ${inputfile}" : "-k ${inputfile}" +- def sample_label = ("$inputfile".endsWith(".fastq.gz") || "$inputfile".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' +- """ +- touch ${prefix}.count.txt +- touch ${prefix}.count_normalized.txt +- cat <<-END_VERSIONS > versions.yml +- "${task.process}": +- mageck: \$(mageck -v) +- END_VERSIONS +- """ +-} ++} ************************************************************ diff --git a/modules/nf-core/mageck/count/main.nf b/modules/nf-core/mageck/count/main.nf index 119ea961..f81b1d8e 100644 --- a/modules/nf-core/mageck/count/main.nf +++ b/modules/nf-core/mageck/count/main.nf @@ -2,10 +2,10 @@ process MAGECK_COUNT { tag "$meta.id" label 'process_high' - conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mageck:0.5.9--py37h6bb024c_0': - 'biocontainers/mageck:0.5.9--py37h6bb024c_0' }" + 'https://depot.galaxyproject.org/singularity/mageck:0.5.9.5--py39h1f90b4d_3': + 'biocontainers/mageck:0.5.9.5--py39h1f90b4d_3' }" input: tuple val(meta), path(fastq1), path(fastq2) @@ -25,7 +25,6 @@ process MAGECK_COUNT { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - // def input_file = ("$inputfile".endsWith(".fastq.gz") || "$inputfile".endsWith(".fq.gz")) ? "--fastq ${inputfile}" : "-k ${inputfile}" def sample_label = ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' if (meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) || "$fastq1".endsWith(".bam")) { @@ -49,4 +48,4 @@ process MAGECK_COUNT { mageck: \$(mageck -v) END_VERSIONS """ -} +} \ No newline at end of file diff --git a/modules/nf-core/mageck/count/meta.yml b/modules/nf-core/mageck/count/meta.yml index 3e3ad475..6450de0a 100644 --- a/modules/nf-core/mageck/count/meta.yml +++ b/modules/nf-core/mageck/count/meta.yml @@ -6,12 +6,12 @@ keywords: - sgRNA - CRISPR-Cas9 tools: - - "mageck": + - mageck: description: "MAGeCK (Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout), an algorithm to process, QC, analyze and visualize CRISPR screening data." homepage: "https://sourceforge.net/p/mageck/wiki/Home/" documentation: "https://sourceforge.net/p/mageck/wiki/demo/#step-4-run-the-mageck-count-command" doi: "10.1186/s13059-014-0554-4" - licence: "['BSD License']" + licence: ["BSD License"] input: - meta: type: map diff --git a/modules/nf-core/mageck/count/tests/main.nf.test b/modules/nf-core/mageck/count/tests/main.nf.test new file mode 100644 index 00000000..fbfeaeb6 --- /dev/null +++ b/modules/nf-core/mageck/count/tests/main.nf.test @@ -0,0 +1,60 @@ +nextflow_process { + + name "Test Process MAGECK_COUNT" + script "../main.nf" + process "MAGECK_COUNT" + + tag "modules" + tag "modules_nfcore" + tag "mageck" + tag "mageck/count" + + test("mus_musculus - fastq") { + + when { + process { + """ + input[0] = [ [ id:'test,test2', single_end:true] , // meta map + [file(params.modules_testdata_base_path + 'genomics/mus_musculus/mageck/ERR376998.small.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/mus_musculus/mageck/ERR376999.small.fastq.gz', checkIfExists: true)] + ] + input[1] = file(params.modules_testdata_base_path + 'genomics/mus_musculus/mageck/yusa_library.csv') + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("mus_musculus - fastq - stub" ) { + + options "-stub" + + when { + process { + """ + input[0] = [ [ id:'test,test2', single_end:true] , // meta map + [file(params.modules_testdata_base_path + 'genomics/mus_musculus/mageck/ERR376998.small.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/mus_musculus/mageck/ERR376999.small.fastq.gz', checkIfExists: true)] + ] + input[1] = file(params.modules_testdata_base_path + 'genomics/mus_musculus/mageck/yusa_library.csv') + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() + } + ) + } + + } +} diff --git a/modules/nf-core/mageck/count/tests/main.nf.test.snap b/modules/nf-core/mageck/count/tests/main.nf.test.snap new file mode 100644 index 00000000..31dbc74b --- /dev/null +++ b/modules/nf-core/mageck/count/tests/main.nf.test.snap @@ -0,0 +1,100 @@ +{ + "mus_musculus - fastq": { + "content": [ + { + "0": [ + [ + { + "id": "test,test2", + "single_end": true + }, + "test,test2.count.txt:md5,2759bdc010998f863518cf7a2df812dc" + ] + ], + "1": [ + [ + { + "id": "test,test2", + "single_end": true + }, + "test,test2.count_normalized.txt:md5,d79f3863168ee0f0750a59d94f70ce1a" + ] + ], + "2": [ + "versions.yml:md5,8fd54f61f3c77976003f79397f6b12d9" + ], + "count": [ + [ + { + "id": "test,test2", + "single_end": true + }, + "test,test2.count.txt:md5,2759bdc010998f863518cf7a2df812dc" + ] + ], + "norm": [ + [ + { + "id": "test,test2", + "single_end": true + }, + "test,test2.count_normalized.txt:md5,d79f3863168ee0f0750a59d94f70ce1a" + ] + ], + "versions": [ + "versions.yml:md5,8fd54f61f3c77976003f79397f6b12d9" + ] + } + ], + "timestamp": "2024-06-14T17:31:00.990366" + }, + "mus_musculus - fastq - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test,test2", + "single_end": true + }, + "test,test2.count.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test,test2", + "single_end": true + }, + "test,test2.count_normalized.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,8fd54f61f3c77976003f79397f6b12d9" + ], + "count": [ + [ + { + "id": "test,test2", + "single_end": true + }, + "test,test2.count.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "norm": [ + [ + { + "id": "test,test2", + "single_end": true + }, + "test,test2.count_normalized.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,8fd54f61f3c77976003f79397f6b12d9" + ] + } + ], + "timestamp": "2024-06-14T17:31:12.173269" + } +} \ No newline at end of file diff --git a/modules/nf-core/mageck/count/tests/tags.yml b/modules/nf-core/mageck/count/tests/tags.yml new file mode 100644 index 00000000..b1ee5528 --- /dev/null +++ b/modules/nf-core/mageck/count/tests/tags.yml @@ -0,0 +1,2 @@ +mageck/count: + - "modules/nf-core/mageck/count/**" From 549751d289b09816191ddfbdeb55f0c562b02e2b Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 19 Jun 2024 12:21:52 +0200 Subject: [PATCH 219/226] Update mageck test with nf-test --- modules.json | 2 +- modules/nf-core/mageck/test/environment.yml | 2 +- modules/nf-core/mageck/test/mageck-test.diff | 13 +-- modules/nf-core/mageck/test/main.nf | 19 +++- modules/nf-core/mageck/test/meta.yml | 4 +- .../nf-core/mageck/test/tests/main.nf.test | 92 ++++++++++++++++ .../mageck/test/tests/main.nf.test.snap | 102 ++++++++++++++++++ .../test/tests/nextflow-day0_label.config | 6 ++ .../nf-core/mageck/test/tests/nextflow.config | 6 ++ modules/nf-core/mageck/test/tests/tags.yml | 2 + 10 files changed, 235 insertions(+), 13 deletions(-) create mode 100644 modules/nf-core/mageck/test/tests/main.nf.test create mode 100644 modules/nf-core/mageck/test/tests/main.nf.test.snap create mode 100644 modules/nf-core/mageck/test/tests/nextflow-day0_label.config create mode 100644 modules/nf-core/mageck/test/tests/nextflow.config create mode 100644 modules/nf-core/mageck/test/tests/tags.yml diff --git a/modules.json b/modules.json index b2b81fa2..d0d838f5 100644 --- a/modules.json +++ b/modules.json @@ -81,7 +81,7 @@ }, "mageck/test": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "02ddbebcea3e0aad5ddd090d243e7ceb3ae2f063", "installed_by": [ "modules" ], diff --git a/modules/nf-core/mageck/test/environment.yml b/modules/nf-core/mageck/test/environment.yml index 622038ac..1d056e5a 100644 --- a/modules/nf-core/mageck/test/environment.yml +++ b/modules/nf-core/mageck/test/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::mageck=0.5.9 + - bioconda::mageck=0.5.9.5 diff --git a/modules/nf-core/mageck/test/mageck-test.diff b/modules/nf-core/mageck/test/mageck-test.diff index e4be2cfc..0bab2ebc 100644 --- a/modules/nf-core/mageck/test/mageck-test.diff +++ b/modules/nf-core/mageck/test/mageck-test.diff @@ -8,21 +8,22 @@ Changes in module 'nf-core/mageck/test' label 'process_medium' conda "${moduleDir}/environment.yml" -@@ -14,6 +14,8 @@ +@@ -13,7 +13,9 @@ + output: tuple val(meta), path("*.gene_summary.txt") , emit: gene_summary tuple val(meta), path("*.sgrna_summary.txt") , emit: sgrna_summary - tuple val(meta), path("*.R") , emit: r_script +- tuple val(meta), path("*.R") , emit: r_script, optional: true ++ tuple val(meta), path("*.R") , emit: r_script + tuple val(meta), path("*.Rnw") , emit: r_summary + tuple val(meta), path("*.log") , emit: logs path "versions.yml" , emit: versions when: -@@ -21,14 +23,17 @@ - +@@ -22,13 +24,16 @@ script: def args = task.ext.args ?: '' -+ def args2 = task.ext.args2 ?: '' def prefix = task.ext.prefix ?: "${meta.id}" ++ def args2 = task.ext.args2 ?: '' """ mageck \\ @@ -34,7 +35,7 @@ Changes in module 'nf-core/mageck/test' - -n $prefix + -n ${meta.treatment}_${meta.reference} - cat <<-END_VERSIONS > versions.yml + "${task.process}": ************************************************************ diff --git a/modules/nf-core/mageck/test/main.nf b/modules/nf-core/mageck/test/main.nf index 8f954b80..2036ced6 100644 --- a/modules/nf-core/mageck/test/main.nf +++ b/modules/nf-core/mageck/test/main.nf @@ -4,8 +4,8 @@ process MAGECK_TEST { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mageck:0.5.9--py37h6bb024c_0': - 'biocontainers/mageck:0.5.9--py37h6bb024c_0' }" + 'https://depot.galaxyproject.org/singularity/mageck:0.5.9.5--py39h1f90b4d_3': + 'biocontainers/mageck:0.5.9.5--py39h1f90b4d_3' }" input: tuple val(meta), path(count_table) @@ -23,8 +23,8 @@ process MAGECK_TEST { script: def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + def args2 = task.ext.args2 ?: '' """ mageck \\ @@ -35,6 +35,19 @@ process MAGECK_TEST { -k $count_table \\ -n ${meta.treatment}_${meta.reference} + cat <<-END_VERSIONS > versions.yml + "${task.process}": + mageck: \$(mageck -v) + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.gene_summary.txt + touch ${prefix}.sgrna_summary.txt + touch ${prefix}.R cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/mageck/test/meta.yml b/modules/nf-core/mageck/test/meta.yml index 4749d4d7..d0d93f33 100644 --- a/modules/nf-core/mageck/test/meta.yml +++ b/modules/nf-core/mageck/test/meta.yml @@ -5,13 +5,13 @@ keywords: - rra - CRISPR tools: - - "mageck": + - mageck: description: "MAGeCK (Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout), an algorithm to process, QC, analyze and visualize CRISPR screening data." homepage: "https://sourceforge.net/p/mageck/wiki/Home/#mle" documentation: "https://sourceforge.net/p/mageck/wiki/Home/" tool_dev_url: "https://bitbucket.org/liulab/mageck/src" doi: "10.1186/s13059-015-0843-6" - licence: "['BSD License']" + licence: ["BSD License"] input: - meta: type: map diff --git a/modules/nf-core/mageck/test/tests/main.nf.test b/modules/nf-core/mageck/test/tests/main.nf.test new file mode 100644 index 00000000..45cb3734 --- /dev/null +++ b/modules/nf-core/mageck/test/tests/main.nf.test @@ -0,0 +1,92 @@ +nextflow_process { + + name "Test Process MAGECK_TEST" + script "../main.nf" + process "MAGECK_TEST" + + tag "modules" + tag "modules_nfcore" + tag "mageck" + tag "mageck/test" + + test("mageck-test - csv") { + + config "./nextflow.config" + + when { + process { + """ + input[0] = [ [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/mus_musculus/mageck/count_table.csv', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + file(process.out.gene_summary.get(0).get(1)).readLines()[0], + file(process.out.sgrna_summary.get(0).get(1)).readLines()[0], + file(process.out.r_script.get(0).get(1)).readLines()[0] + ).match() + } + ) + } + + } + + test("mageck-test-day0 - csv") { + + config "./nextflow-day0_label.config" + + when { + process { + """ + input[0] = [ [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/mus_musculus/mageck/count_table.csv', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + file(process.out.gene_summary.get(0).get(1)[0]).readLines()[0], + file(process.out.sgrna_summary.get(0).get(1)[0]).readLines()[0] + ).match() + } + ) + } + + } + + test("mageck-test - csv - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/mus_musculus/mageck/count_table.csv', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/mageck/test/tests/main.nf.test.snap b/modules/nf-core/mageck/test/tests/main.nf.test.snap new file mode 100644 index 00000000..3b69c697 --- /dev/null +++ b/modules/nf-core/mageck/test/tests/main.nf.test.snap @@ -0,0 +1,102 @@ +{ + "mageck-test - csv - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.gene_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.sgrna_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + [ + { + "id": "test", + "single_end": false + }, + "test.R:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,d63cf999d719137c7310a3f5b4e03fd8" + ], + "gene_summary": [ + [ + { + "id": "test", + "single_end": false + }, + "test.gene_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "r_script": [ + [ + { + "id": "test", + "single_end": false + }, + "test.R:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "sgrna_summary": [ + [ + { + "id": "test", + "single_end": false + }, + "test.sgrna_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,d63cf999d719137c7310a3f5b4e03fd8" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.07.0" + }, + "timestamp": "2024-06-17T15:11:41.655784" + }, + "mageck-test - csv": { + "content": [ + [ + "versions.yml:md5,d63cf999d719137c7310a3f5b4e03fd8" + ], + "id\tnum\tneg|score\tneg|p-value\tneg|fdr\tneg|rank\tneg|goodsgrna\tneg|lfc\tpos|score\tpos|p-value\tpos|fdr\tpos|rank\tpos|goodsgrna\tpos|lfc", + "sgrna\tGene\tcontrol_count\ttreatment_count\tcontrol_mean\ttreat_mean\tLFC\tcontrol_var\tadj_var\tscore\tp.low\tp.high\tp.twosided\tFDR\thigh_in_treatment", + "pdf(file='test.pdf',width=4.5,height=4.5);" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.07.0" + }, + "timestamp": "2024-06-17T15:11:06.123141" + }, + "mageck-test-day0 - csv": { + "content": [ + [ + "versions.yml:md5,d63cf999d719137c7310a3f5b4e03fd8" + ], + "id\tnum\tneg|score\tneg|p-value\tneg|fdr\tneg|rank\tneg|goodsgrna\tneg|lfc\tpos|score\tpos|p-value\tpos|fdr\tpos|rank\tpos|goodsgrna\tpos|lfc", + "sgrna\tGene\tcontrol_count\ttreatment_count\tcontrol_mean\ttreat_mean\tLFC\tcontrol_var\tadj_var\tscore\tp.low\tp.high\tp.twosided\tFDR\thigh_in_treatment" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.07.0" + }, + "timestamp": "2024-06-17T15:11:25.915572" + } +} \ No newline at end of file diff --git a/modules/nf-core/mageck/test/tests/nextflow-day0_label.config b/modules/nf-core/mageck/test/tests/nextflow-day0_label.config new file mode 100644 index 00000000..4a33e5fb --- /dev/null +++ b/modules/nf-core/mageck/test/tests/nextflow-day0_label.config @@ -0,0 +1,6 @@ +process { + + withName: 'MAGECK_TEST' { + ext.args = "--day0-label 'HL60.initial' " + } +} diff --git a/modules/nf-core/mageck/test/tests/nextflow.config b/modules/nf-core/mageck/test/tests/nextflow.config new file mode 100644 index 00000000..48f87585 --- /dev/null +++ b/modules/nf-core/mageck/test/tests/nextflow.config @@ -0,0 +1,6 @@ +process { + + withName: 'MAGECK_TEST' { + ext.args = "-t 'HL60.initial' " + } +} diff --git a/modules/nf-core/mageck/test/tests/tags.yml b/modules/nf-core/mageck/test/tests/tags.yml new file mode 100644 index 00000000..1910c717 --- /dev/null +++ b/modules/nf-core/mageck/test/tests/tags.yml @@ -0,0 +1,2 @@ +mageck/test: + - "modules/nf-core/mageck/test/**" From 1eed36c13537bd9ab248769a69ebca681e8bc8c5 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 19 Jun 2024 12:42:09 +0200 Subject: [PATCH 220/226] Update mageck test and mageck mle --- modules.json | 2 +- modules/nf-core/mageck/mle/environment.yml | 2 +- modules/nf-core/mageck/mle/mageck-mle.diff | 37 +++++----- modules/nf-core/mageck/mle/main.nf | 6 +- modules/nf-core/mageck/mle/meta.yml | 2 +- modules/nf-core/mageck/mle/tests/main.nf.test | 67 +++++++++++++++++++ .../mageck/mle/tests/main.nf.test.snap | 67 +++++++++++++++++++ modules/nf-core/mageck/mle/tests/tags.yml | 2 + 8 files changed, 164 insertions(+), 21 deletions(-) create mode 100644 modules/nf-core/mageck/mle/tests/main.nf.test create mode 100644 modules/nf-core/mageck/mle/tests/main.nf.test.snap create mode 100644 modules/nf-core/mageck/mle/tests/tags.yml diff --git a/modules.json b/modules.json index d0d838f5..a1df6e43 100644 --- a/modules.json +++ b/modules.json @@ -73,7 +73,7 @@ }, "mageck/mle": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "a179c03b8933e96cdf232e8de9addf5c83a05d24", "installed_by": [ "modules" ], diff --git a/modules/nf-core/mageck/mle/environment.yml b/modules/nf-core/mageck/mle/environment.yml index 27d8c762..ed2cf001 100644 --- a/modules/nf-core/mageck/mle/environment.yml +++ b/modules/nf-core/mageck/mle/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::mageck=0.5.9 + - bioconda::mageck=0.5.9.5 diff --git a/modules/nf-core/mageck/mle/mageck-mle.diff b/modules/nf-core/mageck/mle/mageck-mle.diff index d8a988f1..1d04b5c4 100644 --- a/modules/nf-core/mageck/mle/mageck-mle.diff +++ b/modules/nf-core/mageck/mle/mageck-mle.diff @@ -1,19 +1,8 @@ Changes in module 'nf-core/mageck/mle' --- modules/nf-core/mageck/mle/main.nf +++ modules/nf-core/mageck/mle/main.nf -@@ -1,15 +1,14 @@ - process MAGECK_MLE { -- tag "$meta.id" -- label 'process_medium' -+ tag "$prefix" -+ label 'process_high' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? -- 'https://depot.galaxyproject.org/singularity/mageck:0.5.9--py37h6bb024c_0': -- 'biocontainers/mageck:0.5.9--py37h6bb024c_0' }" -+ 'https://depot.galaxyproject.org/singularity/mageck:0.5.9.5--py39h1f90b4d_3': -+ 'biocontainers/mageck:0.5.9.5--py39h1f90b4d_3' }" +@@ -8,8 +8,7 @@ + 'biocontainers/mageck:0.5.9.5--py39h1f90b4d_3' }" input: - tuple val(meta), path(count_table) @@ -32,7 +21,7 @@ Changes in module 'nf-core/mageck/mle' """ mageck \\ -@@ -29,8 +29,9 @@ +@@ -29,26 +29,13 @@ $args \\ --threads $task.cpus \\ -k $count_table \\ @@ -44,5 +33,23 @@ Changes in module 'nf-core/mageck/mle' cat <<-END_VERSIONS > versions.yml "${task.process}": - + mageck: \$(mageck -v) + END_VERSIONS + """ +- stub: +- def prefix = task.ext.prefix ?: "${meta.id}" +- +- """ +- touch ${prefix}.gene_summary.txt +- touch ${prefix}.sgrna_summary.txt +- +- cat <<-END_VERSIONS > versions.yml +- "${task.process}": +- mageck: \$(mageck -v) +- END_VERSIONS +- """ +- +- +-} ++} ************************************************************ diff --git a/modules/nf-core/mageck/mle/main.nf b/modules/nf-core/mageck/mle/main.nf index 049254a3..458f7ba4 100644 --- a/modules/nf-core/mageck/mle/main.nf +++ b/modules/nf-core/mageck/mle/main.nf @@ -1,6 +1,6 @@ process MAGECK_MLE { - tag "$prefix" - label 'process_high' + tag "$meta.id" + label 'process_medium' conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? @@ -38,4 +38,4 @@ process MAGECK_MLE { mageck: \$(mageck -v) END_VERSIONS """ -} +} \ No newline at end of file diff --git a/modules/nf-core/mageck/mle/meta.yml b/modules/nf-core/mageck/mle/meta.yml index bdb3d0cb..0b944aaf 100644 --- a/modules/nf-core/mageck/mle/meta.yml +++ b/modules/nf-core/mageck/mle/meta.yml @@ -11,7 +11,7 @@ tools: documentation: "https://sourceforge.net/p/mageck/wiki/Home/" tool_dev_url: "https://bitbucket.org/liulab/mageck/src" doi: "10.1186/s13059-015-0843-6" - licence: "['BSD License']" + licence: ["BSD"] input: - meta: type: map diff --git a/modules/nf-core/mageck/mle/tests/main.nf.test b/modules/nf-core/mageck/mle/tests/main.nf.test new file mode 100644 index 00000000..5b20fd44 --- /dev/null +++ b/modules/nf-core/mageck/mle/tests/main.nf.test @@ -0,0 +1,67 @@ +nextflow_process { + + name "Test Process MAGECK_MLE" + script "../main.nf" + process "MAGECK_MLE" + + tag "modules" + tag "modules_nfcore" + tag "mageck" + tag "mageck/mle" + + test("mageck_mle - csv") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['mus_musculus']['csv']['count_table'], checkIfExists: true) + ] + input[1] = + file(params.test_data['mus_musculus']['txt']['design_matrix'], + checkIfExists: true) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.versions).match() }, + { assert file(process.out.gene_summary[0][1]).readLines().first().contains("Gene") }, + { assert file(process.out.sgrna_summary[0][1]).exists() } + ) + + } + + } + + test("mageck_mle - csv - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['mus_musculus']['csv']['count_table'], checkIfExists: true) + ] + input[1] = + file(params.test_data['mus_musculus']['txt']['design_matrix'], + checkIfExists: true) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/mageck/mle/tests/main.nf.test.snap b/modules/nf-core/mageck/mle/tests/main.nf.test.snap new file mode 100644 index 00000000..6fb2101d --- /dev/null +++ b/modules/nf-core/mageck/mle/tests/main.nf.test.snap @@ -0,0 +1,67 @@ +{ + "mageck_mle - csv - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.gene_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.sgrna_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,d83f4ee1ef0e972c5ea9a8deee7ba3b3" + ], + "gene_summary": [ + [ + { + "id": "test", + "single_end": false + }, + "test.gene_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "sgrna_summary": [ + [ + { + "id": "test", + "single_end": false + }, + "test.sgrna_summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,d83f4ee1ef0e972c5ea9a8deee7ba3b3" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-04T13:27:46.709493836" + }, + "mageck_mle - csv": { + "content": [ + [ + "versions.yml:md5,d83f4ee1ef0e972c5ea9a8deee7ba3b3" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-04T10:55:23.297715246" + } +} \ No newline at end of file diff --git a/modules/nf-core/mageck/mle/tests/tags.yml b/modules/nf-core/mageck/mle/tests/tags.yml new file mode 100644 index 00000000..97929cb1 --- /dev/null +++ b/modules/nf-core/mageck/mle/tests/tags.yml @@ -0,0 +1,2 @@ +mageck/mle: + - "modules/nf-core/mageck/mle/**" From 51edeaa4dd8e793d496995b4ca3d64da1c6720eb Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 19 Jun 2024 12:46:42 +0200 Subject: [PATCH 221/226] Ran pre commit --- modules.json | 86 ++++++++++++++-------------------------------------- 1 file changed, 22 insertions(+), 64 deletions(-) diff --git a/modules.json b/modules.json index a1df6e43..8e33a623 100644 --- a/modules.json +++ b/modules.json @@ -8,157 +8,115 @@ "bowtie2/align": { "branch": "master", "git_sha": "0fe30831abbc2ed115e46e92330edf38f56edc3d", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bowtie2/build": { "branch": "master", "git_sha": "1fea64f5132a813ec97c1c6d3a74e0aee7142b6d", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/index": { "branch": "master", "git_sha": "086fa66260595e123b0ea47a6512539b72a9afa3", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", "git_sha": "0c34b8159f62cde451c4ff249629c9d0a4f3f9c3", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cat/fastq": { "branch": "master", "git_sha": "4fc983ad0b30e6e32696fa7d980c76c7bfe1c03e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" }, "cutadapt": { "branch": "master", "git_sha": "6618151ed69274863dc6fe6d2920afa90abaca1f", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/cutadapt/cutadapt.diff" }, "fastqc": { "branch": "master", "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "mageck/count": { "branch": "master", "git_sha": "3fd600f14c03766f840acf29e1a6a1bc45d5c9a0", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/count/mageck-count.diff" }, "mageck/mle": { "branch": "master", "git_sha": "a179c03b8933e96cdf232e8de9addf5c83a05d24", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" }, "mageck/test": { "branch": "master", "git_sha": "02ddbebcea3e0aad5ddd090d243e7ceb3ae2f063", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/test/mageck-test.diff" }, "medaka": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/medaka/medaka.diff" }, "minimap2/align": { "branch": "master", "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" }, "minimap2/index": { "branch": "master", "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "pear": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "racon": { "branch": "master", "git_sha": "f5ed3ac0834b68e80a00a06a61d04ce8e896f275", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/index": { "branch": "master", "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "seqtk/seq": { "branch": "master", "git_sha": "7f88aae93c69586c0789322b77743ee0ef469502", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "vsearch/cluster": { "branch": "master", "git_sha": "d97b335eb448073c1b680710303c02a55f40c77c", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" }, "vsearch/sort": { "branch": "master", "git_sha": "97cf4c4a37a8931e19ef728de92f930a366669f2", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } }, @@ -167,4 +125,4 @@ } } } -} \ No newline at end of file +} From 1cefe9ab2d870562fbba3ee01b0c7701783df079 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 19 Jun 2024 12:54:46 +0200 Subject: [PATCH 222/226] Update mageck count --- modules.json | 86 ++++++++++++++----- .../nf-core/mageck/count/mageck-count.diff | 14 +-- modules/nf-core/mageck/count/main.nf | 1 + 3 files changed, 72 insertions(+), 29 deletions(-) diff --git a/modules.json b/modules.json index 8e33a623..a1df6e43 100644 --- a/modules.json +++ b/modules.json @@ -8,115 +8,157 @@ "bowtie2/align": { "branch": "master", "git_sha": "0fe30831abbc2ed115e46e92330edf38f56edc3d", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bowtie2/build": { "branch": "master", "git_sha": "1fea64f5132a813ec97c1c6d3a74e0aee7142b6d", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/index": { "branch": "master", "git_sha": "086fa66260595e123b0ea47a6512539b72a9afa3", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/mem": { "branch": "master", "git_sha": "0c34b8159f62cde451c4ff249629c9d0a4f3f9c3", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cat/fastq": { "branch": "master", "git_sha": "4fc983ad0b30e6e32696fa7d980c76c7bfe1c03e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" }, "cutadapt": { "branch": "master", "git_sha": "6618151ed69274863dc6fe6d2920afa90abaca1f", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/cutadapt/cutadapt.diff" }, "fastqc": { "branch": "master", "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "mageck/count": { "branch": "master", "git_sha": "3fd600f14c03766f840acf29e1a6a1bc45d5c9a0", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/count/mageck-count.diff" }, "mageck/mle": { "branch": "master", "git_sha": "a179c03b8933e96cdf232e8de9addf5c83a05d24", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" }, "mageck/test": { "branch": "master", "git_sha": "02ddbebcea3e0aad5ddd090d243e7ceb3ae2f063", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/test/mageck-test.diff" }, "medaka": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/medaka/medaka.diff" }, "minimap2/align": { "branch": "master", "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" }, "minimap2/index": { "branch": "master", "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "pear": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "racon": { "branch": "master", "git_sha": "f5ed3ac0834b68e80a00a06a61d04ce8e896f275", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/index": { "branch": "master", "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "seqtk/seq": { "branch": "master", "git_sha": "7f88aae93c69586c0789322b77743ee0ef469502", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "vsearch/cluster": { "branch": "master", "git_sha": "d97b335eb448073c1b680710303c02a55f40c77c", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" }, "vsearch/sort": { "branch": "master", "git_sha": "97cf4c4a37a8931e19ef728de92f930a366669f2", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] } } }, @@ -125,4 +167,4 @@ } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/mageck/count/mageck-count.diff b/modules/nf-core/mageck/count/mageck-count.diff index a7a7d72d..a701e661 100644 --- a/modules/nf-core/mageck/count/mageck-count.diff +++ b/modules/nf-core/mageck/count/mageck-count.diff @@ -1,16 +1,16 @@ Changes in module 'nf-core/mageck/count' --- modules/nf-core/mageck/count/main.nf +++ modules/nf-core/mageck/count/main.nf -@@ -1,19 +1,22 @@ +@@ -1,6 +1,7 @@ process MAGECK_COUNT { tag "$meta.id" - label 'process_medium' + label 'process_high' - -- conda "${moduleDir}/environment.yml" + + + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mageck:0.5.9.5--py39h1f90b4d_3': +@@ -8,12 +9,15 @@ 'biocontainers/mageck:0.5.9.5--py39h1f90b4d_3' }" input: @@ -28,7 +28,7 @@ Changes in module 'nf-core/mageck/count' path "versions.yml" , emit: versions when: -@@ -22,8 +25,13 @@ +@@ -22,8 +26,13 @@ script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" @@ -44,7 +44,7 @@ Changes in module 'nf-core/mageck/count' """ mageck \\ -@@ -32,7 +40,7 @@ +@@ -32,7 +41,7 @@ -l $library \\ -n $prefix \\ $sample_label \\ @@ -53,7 +53,7 @@ Changes in module 'nf-core/mageck/count' cat <<-END_VERSIONS > versions.yml -@@ -40,17 +48,4 @@ +@@ -40,17 +49,4 @@ mageck: \$(mageck -v) END_VERSIONS """ diff --git a/modules/nf-core/mageck/count/main.nf b/modules/nf-core/mageck/count/main.nf index f81b1d8e..cddcbcf6 100644 --- a/modules/nf-core/mageck/count/main.nf +++ b/modules/nf-core/mageck/count/main.nf @@ -3,6 +3,7 @@ process MAGECK_COUNT { label 'process_high' + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mageck:0.5.9.5--py39h1f90b4d_3': 'biocontainers/mageck:0.5.9.5--py39h1f90b4d_3' }" From 05ade2fb8b58f0e1917bfbd138e41c54479e0b0a Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 19 Jun 2024 12:57:44 +0200 Subject: [PATCH 223/226] ran prettier --- modules.json | 86 ++++++++++++++-------------------------------------- 1 file changed, 22 insertions(+), 64 deletions(-) diff --git a/modules.json b/modules.json index a1df6e43..8e33a623 100644 --- a/modules.json +++ b/modules.json @@ -8,157 +8,115 @@ "bowtie2/align": { "branch": "master", "git_sha": "0fe30831abbc2ed115e46e92330edf38f56edc3d", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bowtie2/build": { "branch": "master", "git_sha": "1fea64f5132a813ec97c1c6d3a74e0aee7142b6d", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/index": { "branch": "master", "git_sha": "086fa66260595e123b0ea47a6512539b72a9afa3", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", "git_sha": "0c34b8159f62cde451c4ff249629c9d0a4f3f9c3", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cat/fastq": { "branch": "master", "git_sha": "4fc983ad0b30e6e32696fa7d980c76c7bfe1c03e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" }, "cutadapt": { "branch": "master", "git_sha": "6618151ed69274863dc6fe6d2920afa90abaca1f", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/cutadapt/cutadapt.diff" }, "fastqc": { "branch": "master", "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "mageck/count": { "branch": "master", "git_sha": "3fd600f14c03766f840acf29e1a6a1bc45d5c9a0", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/count/mageck-count.diff" }, "mageck/mle": { "branch": "master", "git_sha": "a179c03b8933e96cdf232e8de9addf5c83a05d24", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" }, "mageck/test": { "branch": "master", "git_sha": "02ddbebcea3e0aad5ddd090d243e7ceb3ae2f063", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/test/mageck-test.diff" }, "medaka": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/medaka/medaka.diff" }, "minimap2/align": { "branch": "master", "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" }, "minimap2/index": { "branch": "master", "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "pear": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "racon": { "branch": "master", "git_sha": "f5ed3ac0834b68e80a00a06a61d04ce8e896f275", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/index": { "branch": "master", "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "seqtk/seq": { "branch": "master", "git_sha": "7f88aae93c69586c0789322b77743ee0ef469502", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "vsearch/cluster": { "branch": "master", "git_sha": "d97b335eb448073c1b680710303c02a55f40c77c", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" }, "vsearch/sort": { "branch": "master", "git_sha": "97cf4c4a37a8931e19ef728de92f930a366669f2", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } }, @@ -167,4 +125,4 @@ } } } -} \ No newline at end of file +} From ffae1c76ba1089a8119b49a4720b21ac5990cc05 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 19 Jun 2024 14:59:30 +0200 Subject: [PATCH 224/226] Add stub for mageck count --- modules.json | 86 ++++++++++++++----- .../nf-core/mageck/count/mageck-count.diff | 34 +++++--- modules/nf-core/mageck/count/main.nf | 20 +++++ 3 files changed, 104 insertions(+), 36 deletions(-) diff --git a/modules.json b/modules.json index 8e33a623..a1df6e43 100644 --- a/modules.json +++ b/modules.json @@ -8,115 +8,157 @@ "bowtie2/align": { "branch": "master", "git_sha": "0fe30831abbc2ed115e46e92330edf38f56edc3d", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bowtie2/build": { "branch": "master", "git_sha": "1fea64f5132a813ec97c1c6d3a74e0aee7142b6d", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/index": { "branch": "master", "git_sha": "086fa66260595e123b0ea47a6512539b72a9afa3", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/mem": { "branch": "master", "git_sha": "0c34b8159f62cde451c4ff249629c9d0a4f3f9c3", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cat/fastq": { "branch": "master", "git_sha": "4fc983ad0b30e6e32696fa7d980c76c7bfe1c03e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" }, "cutadapt": { "branch": "master", "git_sha": "6618151ed69274863dc6fe6d2920afa90abaca1f", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/cutadapt/cutadapt.diff" }, "fastqc": { "branch": "master", "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "mageck/count": { "branch": "master", "git_sha": "3fd600f14c03766f840acf29e1a6a1bc45d5c9a0", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/count/mageck-count.diff" }, "mageck/mle": { "branch": "master", "git_sha": "a179c03b8933e96cdf232e8de9addf5c83a05d24", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" }, "mageck/test": { "branch": "master", "git_sha": "02ddbebcea3e0aad5ddd090d243e7ceb3ae2f063", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/mageck/test/mageck-test.diff" }, "medaka": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/medaka/medaka.diff" }, "minimap2/align": { "branch": "master", "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" }, "minimap2/index": { "branch": "master", "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "pear": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "racon": { "branch": "master", "git_sha": "f5ed3ac0834b68e80a00a06a61d04ce8e896f275", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/index": { "branch": "master", "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "seqtk/seq": { "branch": "master", "git_sha": "7f88aae93c69586c0789322b77743ee0ef469502", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "vsearch/cluster": { "branch": "master", "git_sha": "d97b335eb448073c1b680710303c02a55f40c77c", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" }, "vsearch/sort": { "branch": "master", "git_sha": "97cf4c4a37a8931e19ef728de92f930a366669f2", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] } } }, @@ -125,4 +167,4 @@ } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/mageck/count/mageck-count.diff b/modules/nf-core/mageck/count/mageck-count.diff index a701e661..fa342b5a 100644 --- a/modules/nf-core/mageck/count/mageck-count.diff +++ b/modules/nf-core/mageck/count/mageck-count.diff @@ -53,23 +53,29 @@ Changes in module 'nf-core/mageck/count' cat <<-END_VERSIONS > versions.yml -@@ -40,17 +49,4 @@ +@@ -43,14 +52,21 @@ + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" +- def input_file = ("$inputfile".endsWith(".fastq.gz")) ? "--fastq ${inputfile}" : "-k ${inputfile}" +- def sample_label = ("$inputfile".endsWith(".fastq.gz") || "$inputfile".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' ++ def sample_label = ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' ++ ++ if (meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) || "$fastq1".endsWith(".bam")) { ++ input = "--fastq $fastq1" ++ } else { ++ input = "--fastq $fastq1 --fastq-2 $fastq2" ++ } + """ + touch ${prefix}.count.txt + touch ${prefix}.count_normalized.txt ++ touch ${prefix}.countsummary.txt ++ touch ${prefix}.log + cat <<-END_VERSIONS > versions.yml + "${task.process}": mageck: \$(mageck -v) END_VERSIONS """ -- stub: -- def args = task.ext.args ?: '' -- def prefix = task.ext.prefix ?: "${meta.id}" -- def input_file = ("$inputfile".endsWith(".fastq.gz")) ? "--fastq ${inputfile}" : "-k ${inputfile}" -- def sample_label = ("$inputfile".endsWith(".fastq.gz") || "$inputfile".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' -- """ -- touch ${prefix}.count.txt -- touch ${prefix}.count_normalized.txt -- cat <<-END_VERSIONS > versions.yml -- "${task.process}": -- mageck: \$(mageck -v) -- END_VERSIONS -- """ -} +} ************************************************************ diff --git a/modules/nf-core/mageck/count/main.nf b/modules/nf-core/mageck/count/main.nf index cddcbcf6..76a0b186 100644 --- a/modules/nf-core/mageck/count/main.nf +++ b/modules/nf-core/mageck/count/main.nf @@ -44,6 +44,26 @@ process MAGECK_COUNT { $input \\ + cat <<-END_VERSIONS > versions.yml + "${task.process}": + mageck: \$(mageck -v) + END_VERSIONS + """ + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def sample_label = ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) ? "--sample-label ${meta.id}" : '' + + if (meta.single_end && ("$fastq1".endsWith(".fastq.gz") || "$fastq1".endsWith(".fq.gz")) || "$fastq1".endsWith(".bam")) { + input = "--fastq $fastq1" + } else { + input = "--fastq $fastq1 --fastq-2 $fastq2" + } + """ + touch ${prefix}.count.txt + touch ${prefix}.count_normalized.txt + touch ${prefix}.countsummary.txt + touch ${prefix}.log cat <<-END_VERSIONS > versions.yml "${task.process}": mageck: \$(mageck -v) From b3b9a9409af249a57c3a92f400918efcd6c4c600 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 19 Jun 2024 15:04:01 +0200 Subject: [PATCH 225/226] add stub in mageck mle --- modules/nf-core/mageck/mle/mageck-mle.diff | 16 +++------------- modules/nf-core/mageck/mle/main.nf | 13 +++++++++++++ 2 files changed, 16 insertions(+), 13 deletions(-) diff --git a/modules/nf-core/mageck/mle/mageck-mle.diff b/modules/nf-core/mageck/mle/mageck-mle.diff index 1d04b5c4..9cb169b0 100644 --- a/modules/nf-core/mageck/mle/mageck-mle.diff +++ b/modules/nf-core/mageck/mle/mageck-mle.diff @@ -21,7 +21,7 @@ Changes in module 'nf-core/mageck/mle' """ mageck \\ -@@ -29,26 +29,13 @@ +@@ -29,8 +29,9 @@ $args \\ --threads $task.cpus \\ -k $count_table \\ @@ -33,23 +33,13 @@ Changes in module 'nf-core/mageck/mle' cat <<-END_VERSIONS > versions.yml "${task.process}": +@@ -49,6 +50,5 @@ mageck: \$(mageck -v) END_VERSIONS """ -- stub: -- def prefix = task.ext.prefix ?: "${meta.id}" -- -- """ -- touch ${prefix}.gene_summary.txt -- touch ${prefix}.sgrna_summary.txt -- -- cat <<-END_VERSIONS > versions.yml -- "${task.process}": -- mageck: \$(mageck -v) -- END_VERSIONS -- """ - - -} ++ +} ************************************************************ diff --git a/modules/nf-core/mageck/mle/main.nf b/modules/nf-core/mageck/mle/main.nf index 458f7ba4..fac59c23 100644 --- a/modules/nf-core/mageck/mle/main.nf +++ b/modules/nf-core/mageck/mle/main.nf @@ -38,4 +38,17 @@ process MAGECK_MLE { mageck: \$(mageck -v) END_VERSIONS """ + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + touch ${prefix}.gene_summary.txt + touch ${prefix}.sgrna_summary.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + mageck: \$(mageck -v) + END_VERSIONS + """ + } \ No newline at end of file From 8ecfd79dbcd8ae36fc448d795ba85f0e0230cdc6 Mon Sep 17 00:00:00 2001 From: laurencekuhl Date: Wed, 19 Jun 2024 15:11:17 +0200 Subject: [PATCH 226/226] Ran prettier --- modules.json | 86 ++++++++++++++-------------------------------------- 1 file changed, 22 insertions(+), 64 deletions(-) diff --git a/modules.json b/modules.json index a1df6e43..8e33a623 100644 --- a/modules.json +++ b/modules.json @@ -8,157 +8,115 @@ "bowtie2/align": { "branch": "master", "git_sha": "0fe30831abbc2ed115e46e92330edf38f56edc3d", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bowtie2/build": { "branch": "master", "git_sha": "1fea64f5132a813ec97c1c6d3a74e0aee7142b6d", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/index": { "branch": "master", "git_sha": "086fa66260595e123b0ea47a6512539b72a9afa3", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", "git_sha": "0c34b8159f62cde451c4ff249629c9d0a4f3f9c3", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cat/fastq": { "branch": "master", "git_sha": "4fc983ad0b30e6e32696fa7d980c76c7bfe1c03e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "crisprcleanr/normalize": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/crisprcleanr/normalize/crisprcleanr-normalize.diff" }, "cutadapt": { "branch": "master", "git_sha": "6618151ed69274863dc6fe6d2920afa90abaca1f", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/cutadapt/cutadapt.diff" }, "fastqc": { "branch": "master", "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "mageck/count": { "branch": "master", "git_sha": "3fd600f14c03766f840acf29e1a6a1bc45d5c9a0", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/count/mageck-count.diff" }, "mageck/mle": { "branch": "master", "git_sha": "a179c03b8933e96cdf232e8de9addf5c83a05d24", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/mle/mageck-mle.diff" }, "mageck/test": { "branch": "master", "git_sha": "02ddbebcea3e0aad5ddd090d243e7ceb3ae2f063", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/mageck/test/mageck-test.diff" }, "medaka": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/medaka/medaka.diff" }, "minimap2/align": { "branch": "master", "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/minimap2/align/minimap2-align.diff" }, "minimap2/index": { "branch": "master", "git_sha": "72e277acfd9e61a9f1368eafb4a9e83f5bcaa9f5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "pear": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "racon": { "branch": "master", "git_sha": "f5ed3ac0834b68e80a00a06a61d04ce8e896f275", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/index": { "branch": "master", "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "seqtk/seq": { "branch": "master", "git_sha": "7f88aae93c69586c0789322b77743ee0ef469502", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "vsearch/cluster": { "branch": "master", "git_sha": "d97b335eb448073c1b680710303c02a55f40c77c", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/vsearch/cluster/vsearch-cluster.diff" }, "vsearch/sort": { "branch": "master", "git_sha": "97cf4c4a37a8931e19ef728de92f930a366669f2", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } }, @@ -167,4 +125,4 @@ } } } -} \ No newline at end of file +}

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