Releases: nextgenusfs/amptk
Releases · nextgenusfs/amptk
ufits v0.5.0
- update to
ufits cluster
to correctly output chimeras detected during clustering and reference filtering - update to automatically detect delimiters in parsing OTU tables
- enhancement to allow for barcode mismatches in the
ufits illumina2
script - note the current implementation is slow and for 99% of uses I don't recommend setting barcode mismatches > 0.
ufits v0.4.9
- remove requirement of otu table for
ufits taxonomy
- add support for dual barcodes for
ufits illumina2
andufits 454
- default merge PE reads for
ufits illumina2
now rescues the forward reads if the pair cannot be merged
ufits v0.4.8
- fix bug in
ufits illumina
where period (.) where not processed correctly in sample names - fix bug in
ufits filter
when passing the--cleanup
option. Totally dumb mistake.... - Add support for creating BIOM v2.1 OTU tables if you have the
biom
package installed. Also added a unified taxonomy 2-column output for each OTU. - added
--cleanup
option toufits illumina
ufits v0.4.7
- fix for pre-processing reads from Illumina platform where custom sequencing primers are used,
ufits illumina
command now handles those datasets better. Note I would not recommend using custom primers, but that is of course up to you... - minor update to docs
ufits v0.4.6
- some bug fixes related to use of USEARCH9, UFITS now supports v9.0.2124 (note: do not use 9.0.2123 has error in -cluster_otus command)
- updates to how UFITS outputs the system info, now cleaner with more info on the OS
- fix bug on failed import for
ufits keep
andufits remove
ufits v0.4.5
*bug fix in ufits taxonomy
where would output csv file - this is only output tsv now
ufits v0.4.4
- bug fix related to downloading databases
ufits v0.4.3
- update to Databases: UNITE v7.1, LSU (based on RDP data), 16S (based on RDP gold), COI (based on BOLD database of arthropods and chordates).
- databases are installed now with
ufits install -i ITS LSU 16S COI
- Robert Edgar now says that chimera reference filtering should use largest database (as opposed to UCHIME paper that says a small curated database is better), thus chimera reference filtering is now configured to do exactly that, options during clustering are:
ufits cluster --uchime_ref [ITS,16S,LSU,COI, custom_path]
ufits cluster_ref
has been updated with the above database information - note this script is still experimental and I would not recommended using it for any environmental data at this point - there may be some targeted usage where it is appropriate- re-write of
ufits taxonomy
to coincide with updates to databases, now you can pass one of the pre-installed databases to the-d, --db
flag or you can specify manually a database using--fasta_db, --usearch_db, and/or --utax_db
ufits v0.4.2
- bug fix to
ufits keep
andufits remove
- bug fix to
ufits taxonomy
where passing a--fasta_db
failed - update to README
ufits v0.4.1
- update to menu of
ufits taxonomy
to be more consistent with rest of the scripts,-i
for input OTU table,-f
for input fasta - improve flexibility of
ufits taxonomy
to work with other groups, removed--only_fungi
and replace with--tax_filter
, i.e.--tax_filter Fungi
. - fix menu in wrapper scripts to reflect changes