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about_data.txt
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########## About the data file (TaqMan chemistry) ##########
### File content
## 12 first rows
#' Header on 7 rows: Global information on the chip (date, machine, parameters...) | not used
#' 2 empty rows
#' Rows 10, 11, 12: columns names (there are generally 11, from A to K) | not used
## Column contents
#' Column A (1): well coordinates in the chip -> S96-A01 stands for sample 96 / assay 01
#' Column B (2): sample name
#' Column C (3): sample type (standard, unknown...)
#' Column D (4): sample concentration. Default value is 1, unless you specify a value when setting up the chip
#' Column E (5): assay name
#' Column F (6): assay type (test, reference...)
#' Column G (7): Ct value
#' Column H (8): relative concentration
#' Column I (9): quality score
#' Column J (10): quality status (Pass or Fail)
#' Column K (11): assay threshold value
#' Column L (12): comments
#' Columns used for analysis: 2, 3, 4, 5, 6, 7, 10
########## About the data file (EvaGreen chemistry) ##########
### File content
## 12 first rows
#' Header on 7 rows: Global information on the chip (date, machine, parameters...) | not used
#' 2 empty rows
#' Rows 10, 11, 12: columns names (there are generally 15, from A to O) | not used
## Column contents
#' Column A (1): well coordinates in the chip -> S96-A01 stands for sample 96 / assay 01
#' Column B (2): sample name
#' Column C (3): sample type (standard, unknown...)
#' Column D (4): sample concentration. Default value is 1, unless you specify a value when setting up the chip
#' Column E (5): assay name
#' Column F (6): assay type (test, reference...)
#' Column G (7): Ct value
#' Column H (8): relative concentration
#' Column I (9): quality score
#' Column J (10): quality status (Pass or Fail)
#' Column K (11): assay threshold value
#' Column L (12): Tm In Range. Melting curve analysis value
#' Column M (13): Tm Out Range
#' Column N (14): Tm Peak Ratio
#' Column O (15): Tm Peak Call (Pass or Fail)
#' Column P (16): User Defined
#' Columns used for analysis: 2, 3, 4, 5, 6, 7, 10