diff --git a/CHANGELOG.md b/CHANGELOG.md
index b3f3fd6..901a06e 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -1,8 +1,8 @@
-# ecSeq/DNAseq
+# genepi/umi-pipeline-nf
---
# Releases
---
# Prereleases
## v0.1.0 -
-* Initialised repo
\ No newline at end of file
+* Initialised repo
diff --git a/README.md b/README.md
index 76d5527..32673e7 100644
--- a/README.md
+++ b/README.md
@@ -1,18 +1,34 @@
-[
](https://www.ecseq.com)
[![Nextflow](https://img.shields.io/badge/nextflow-20.07.1-brightgreen.svg)](https://www.nextflow.io/)
[![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](http://bioconda.github.io/)
-[![Docker](https://img.shields.io/docker/automated/ecseq/dnaseq.svg)](https://hub.docker.com/r/ecseq/dnaseq)
-umi-pipeline-nf Pipeline
+Umi-pipeline-nf
======================
-**umi-pipeline-nf** is based on a [snakemake pipeline](https://github.com/nanoporetech/pipeline-umi-amplicon) provided by [Oxford Nanopore Technologies (ONT)](https://nanoporetech.com/). To increase efficiency and usability the pipeline was transferred to [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation simple and results highly reproducible.
+**Umi-pipeline-nf** creates highly accurate single-molecule consensus sequences for unique molecular identifier (UMI)-tagged amplicon data.
+The pipeline can be run for the whole fastq_pass folder of your nanopore run and, per default, outputs the aligned consensus sequences of each UMI cluster in bam file. The optional variant calling creates a vcf file for all variants that are found in the consensus sequences.
+umi-pipeline-nf is based on the snakemake [ONT UMI analysis pipeline](https://github.com/nanoporetech/pipeline-umi-amplicon) (workflow originally developed by [Karst et al, Nat Biotechnol 18:165–169, 2021](https://www.nature.com/articles/s41592-020-01041-y)). We transferred the pipeline to [Nextflow](https://www.nextflow.io) and included [additional functionalities](#main-adaptations).
-## Overview
-`umi-pipeline-nf` creates highly accurate single-molecule consensus sequences based on amplicon data tagged by unique molecular identifiers (UMIs). The pipeline can be run for the whole fastq_pass folder of your nanopore run and per default, the output are the aligned consensus sequences in bam file format.
-Additional flags can be set to perform a variant calling ( [freebayes](https://github.com/freebayes/freebayes), [lofreq](http://csb5.github.io/lofreq/) or [mutserve](https://mitoverse.readthedocs.io/mutserve/mutserve/) )
+## Workflow
-> See the [output documentation](docs/output.md) for more details of the results.
+1. Input reads are aligned against a reference genome.
+2. The flanking UMI sequences of all reads are extracted.
+3. The extracted UMIs are used to cluster the reads.
+4. Per cluster, highly accurate consensus sequences are created.
+5. The consensus sequences are aligned against the reference sequenced.
+6. An optional variant calling step can be performed.
+
+> See the [output documentation](docs/output.md) for a detailed overview of the pipeline and its output files.
+
+## Main Adaptations
+
+* It comes with docker containers making **installation simple, portable** and **results highly reproducible**.
+* The pipeline is **optimized for parallelization**.
+* Read filtering strategy per UMI cluster was adapted to **preserve the highest quality reads**.
+* **Three commonly used variant callers** ([freebayes](https://github.com/freebayes/freebayes), [lofreq](http://csb5.github.io/lofreq/) or [mutserve](https://mitoverse.readthedocs.io/mutserve/mutserve/)) are supported by the pipeline.
+* The raw reads can be optionally **subsampled**.
+* The raw reads can be **filtered by read length and quality**.
+
+> See the [usage documentation](docs/usage.md) for all of the available parameters of the pipeline.
## Quick Start
@@ -21,19 +37,20 @@ Additional flags can be set to perform a variant calling ( [freebayes](https://g
2. Download the pipeline and test it on a minimal dataset with a single command
```bash
-nextflow run AmstlerStephan/umi-pipeline-nf -profile test,docker
+nextflow run genepi/umi-pipeline-nf -profile test,docker
```
3. Start running your own analysis!
3.1 Download and adapt the config/custom.config with paths to your data (relative and absolute paths possible)
```bash
-nextflow run AmstlerStephan/umi-pipeline-nf -r main -c -profile docker
+nextflow run genepi/umi-pipeline-nf -r main -c -profile docker
```
-> See the [usage documentation](docs/usage.md) for all of the available options when running the pipeline.
-
### Credits
-These scripts were originally written for use by [GENEPI](https://genepi.i-med.ac.at/), by ([@StephanAmstler](https://github.com/AmstlerStephan)).
+The pipeline was written by ([@StephanAmstler](https://github.com/AmstlerStephan)).
+Nextflow template pipeline: [EcSeq](https://github.com/ecSeq).
+Original snakemake-based pipeline: [nanoporetech/pipeline-umi-amplicon](https://github.com/nanoporetech/pipeline-umi-amplicon).
+Original workflow: [SorenKarst/longread_umi](https://github.com/SorenKarst/longread_umi).
diff --git a/bin/bam_to_phred.py b/bin/bam_to_phred.py
deleted file mode 100755
index dde51de..0000000
--- a/bin/bam_to_phred.py
+++ /dev/null
@@ -1,149 +0,0 @@
-import math
-import pysam
-import glob
-import parasail
-import pysam
-import os
-import sys
-import argparse
-
-from tqdm import tqdm
-
-
-
-def parse_args(argv):
- usage = "Annotate homopolymers in FASTA file"
- parser = argparse.ArgumentParser(
- description=usage,
- formatter_class=argparse.RawDescriptionHelpFormatter)
-
- parser.add_argument("BAM",
- type=str,
- nargs=1,
- help="BAM file")
-
- parser.add_argument("BED",
- type=str,
- nargs=1,
- help="BED file")
-
- args = parser.parse_args(argv)
-
- return args
-
-def _do_hit2tel(hit):
- """
- Converts minimap2 hit object to telemetry JSON entry.
-
- :param name: read name
- :param qry_len: int
- :param mean_qscore: float
- :param hit: minimap2 hit object
- :return: dict
- """
- qry_len = hit.query_length
- query_name = hit.query_name
-
- mean_qscore = 0 # _compute_mean_qscore(hit.query_qualities)
-
- summary = {}
- summary['qry_len'] = qry_len
- summary['qry_name'] = query_name
- summary['read_id'] = query_name
- summary['aligner'] = "minimap2"
- summary['mean_qscore'] = mean_qscore
-
- if hit.is_unmapped:
- return None
-
- reference_name = hit.reference_name
-
- el_count = [0] * 10
- for el in hit.cigartuples:
- el_count[el[0]] += el[1]
-
- nn = hit.get_tag("nn")
- nm = hit.get_tag("NM")
- dels = el_count[2]
- ins = el_count[1]
-
- mismatches = nm - dels - ins
- matches = el_count[0] - mismatches
-
- aln_score = 0
-
- accuracy = float(matches) / (matches + nm) * 100
- identity = float(matches) / (matches + mismatches) * 100
- query_alignment_start = hit.query_alignment_start
- query_alignment_end = hit.query_alignment_end
- query_alignment_length = hit.query_alignment_length
-
- ref_alignment_start = hit.reference_start
- ref_alignment_end = hit.reference_end
- ref_alignment_length = hit.reference_length
-
- qry_coverage = float(query_alignment_length) / qry_len * 100
-
- summary['accuracy'] = round(accuracy, 2)
- summary['identity'] = round(identity, 2)
- summary['matches'] = matches #+ mismatches
- summary['mismatches'] = mismatches
- summary['deletions'] = dels
- summary['insertions'] = ins
- summary['aln_len'] = matches + nm
- summary['qry_cov'] = round(qry_coverage, 3)
- summary['ref_name'] = reference_name
- summary['ref_start'] = ref_alignment_start
- summary['ref_end'] = ref_alignment_end
- summary['qry_start'] = query_alignment_start
- summary['qry_end'] = query_alignment_end
- summary['forward'] = not hit.is_reverse
- summary['aln_score'] = aln_score
-
- return summary
-
-
-def main(argv=sys.argv[1:]):
- args = parse_args(argv=argv)
- bam = args.BAM[0]
- bed_file = args.BED[0]
-
- print("read_name", "aplicon_id", "cluster_size", "forward_reads", "reverse_reads", "accuracy", "phred", "phred_low", "read_len", "aln_len", "matches", "mismatches", 'deletions', 'insertions', 'strand', sep='\t')
- with open(bed_file) as bed:
- for line in bed:
- if not line:
- continue
- cols = line.strip().split("\t")
- chrom = cols[0]
- start = int(cols[1]) + 1
- end = int(cols[2]) + 1
- name = cols[3]
- with pysam.AlignmentFile(bam, "rb") as reads:
- for read in reads.fetch(region="{}:{}-{}".format(chrom, start, end)):
- if read.is_supplementary or read.is_secondary:
- continue
-
- results = _do_hit2tel(read)
- #if "_" in results['qry_name']:
- # qname, cluster_size = results['qry_name'].split('_')
- #else:
- qname = results['qry_name']
- cluster_size = 0
-
- fwd = int(cluster_size) / 2
- p = (results['mismatches'] + results['insertions'] + results['deletions']) / results['aln_len']
- if p:
- qscore = -10 * math.log10(p)
- qscore_low = qscore
- else:
- p = (1) / results['aln_len']
- qscore = 'inf'
- qscore_low = -10 * math.log10(p)
- # qscore = int(qscore + 0.5)
-
- print(qname, name, cluster_size, fwd, fwd, results['accuracy'], qscore, qscore_low, results['qry_len'], results['aln_len'], results['matches'], results['mismatches'], results['deletions'], results['insertions'], results['forward'], sep='\t')
-
-
-if __name__ == '__main__':
- main()
-
diff --git a/bin/extract_umis.py b/bin/extract_umis.py
index 81cfefc..6ab02b3 100755
--- a/bin/extract_umis.py
+++ b/bin/extract_umis.py
@@ -52,13 +52,26 @@ def parse_args(argv):
help="Length of adapter",
)
parser.add_argument(
- "-t", "--threads", dest="THREADS", type=int, default=1, help="Number of threads."
+ "-t",
+ "--threads",
+ dest="THREADS",
+ type=int,
+ default=1,
+ help="Number of threads."
)
parser.add_argument(
- "--tsv", dest="TSV", action="store_true", help="write TSV output file"
+ "--tsv",
+ dest="TSV",
+ action="store_true",
+ help="write TSV output file"
)
parser.add_argument(
- "-o", "--output", dest="OUT", type=str, required=False, help="Output directory"
+ "-o",
+ "--output",
+ dest="OUT",
+ type=str,
+ required=False,
+ help="Output directory"
)
parser.add_argument(
"--output_format",
@@ -82,7 +95,10 @@ def parse_args(argv):
help="Reverse UMI sequence",
)
parser.add_argument(
- "INPUT_FA", type=str, default="/dev/stdin", help="Filtered Reads"
+ "INPUT_FA",
+ type=str,
+ default="/dev/stdin",
+ help="Filtered Reads"
)
args = parser.parse_args(argv)
@@ -109,8 +125,10 @@ def extract_umi(query_seq, query_qual, pattern, max_edit_dist, format):
edit_dist = result["editDistance"]
locs = result["locations"][0]
umi = query_seq[locs[0]:locs[1]+1]
+
if format == "fastq":
umi_qual = query_qual[locs[0]:locs[1]+1]
+
return edit_dist, umi, umi_qual
@@ -123,15 +141,18 @@ def extract_adapters(entry, max_adapter_length, format):
if len(entry.sequence) > max_adapter_length:
read_5p_seq = entry.sequence[:max_adapter_length]
read_3p_seq = entry.sequence[-max_adapter_length:]
+
if format == "fastq":
read_5p_qual = entry.quality[:max_adapter_length]
read_3p_qual = entry.quality[-max_adapter_length:]
return read_5p_seq, read_3p_seq, read_5p_qual, read_3p_qual
+
def get_read_name(entry):
return entry.name.split(";")[0]
+
def get_read_strand(entry):
strand = entry.name.split("strand=")
if len(strand) > 1:
@@ -140,6 +161,7 @@ def get_read_strand(entry):
else:
return "+"
+
def combine_umis_fasta(seq_5p, seq_3p, strand):
if strand == "+":
return seq_5p + seq_3p
diff --git a/bin/setup.py b/bin/setup.py
index ecfd4f6..a0501ba 100755
--- a/bin/setup.py
+++ b/bin/setup.py
@@ -15,7 +15,6 @@
description='Toolset to work with ONT amplicon sequencing using UMIs',
zip_safe=False,
install_requires=[
- 'tqdm',
'pysam',
'numpy',
'pandas',
@@ -32,7 +31,6 @@
'umi_extract = umi_amplicon_tools.extract_umis:main',
'umi_reformat_consensus = umi_amplicon_tools.reformat_consensus:main',
'umi_parse_clusters = umi_amplicon_tools.parse_clusters:main',
- 'umi_bam_to_phred = umi_amplicon_tools.bam_to_phred:main',
'umi_stats = umi_amplicon_tools.umi_stats:main'
]
},
diff --git a/bin/umi_stats.py b/bin/umi_stats.py
deleted file mode 100755
index b46b8c5..0000000
--- a/bin/umi_stats.py
+++ /dev/null
@@ -1,260 +0,0 @@
-import argparse
-import pysam
-import sys
-
-import matplotlib.pyplot as plt
-import numpy as np
-import pandas as pd
-import seaborn as sns
-
-
-def parse_args(argv):
- usage = "Annotate homopolymers in FASTA file"
- parser = argparse.ArgumentParser(
- description=usage, formatter_class=argparse.RawDescriptionHelpFormatter
- )
-
- parser.add_argument("-m", "--min", dest="MIN", help="Min read count for clusters")
- parser.add_argument("-M", "--max", dest="MAX", help="Max read count for clusters")
- parser.add_argument(
- "-n",
- "--name",
- dest="NAME",
- help="Amplicon name. Name used in the target BED file.)",
- )
- parser.add_argument(
- "-f", "--figures", dest="FIGURES", help="Prefix for output figures"
- )
- parser.add_argument(
- "FOLDERS", type=str, nargs="+", help="UMI pipeline output folder"
- )
-
- args = parser.parse_args(argv)
-
- return args
-
-
-def get_readstast_bam(folder):
- bam_1d_file = "{}/align/1d.bam".format(folder)
-
- mapped = 0
- unmapped = 0
- with pysam.AlignmentFile(bam_1d_file, "rb") as bam_1d:
- for r in bam_1d:
- if r.is_unmapped:
- unmapped += 1
- continue
- if not r.is_supplementary and not r.is_secondary:
- mapped += 1
- sequenced = mapped + unmapped
- return sequenced, mapped, unmapped
-
-
-def count_fastx_reads_with_umi(folder, amplicon_name):
- fastq_with_umi_file = "{}/fasta_umi/{}_detected_umis.fasta".format(
- folder, amplicon_name
- )
- return count_fastx_reads(fastq_with_umi_file)
-
-
-def count_fastx_reads_on_target(folder, amplicon_name):
- fastq_ontarget_file = "{}/fasta_filtered/{}.fastq".format(folder, amplicon_name)
- return count_fastx_reads(fastq_ontarget_file)
-
-
-def count_fastx_reads(filename):
- count = 0
- with pysam.FastxFile(filename) as fh:
- for entry in fh:
- count += 1
- return count
-
-
-def get_median_acc(folder, amplicon_name):
- acc_stats_file = "{}/stats/{}_consensus_size_vs_acc.tsv".format(
- folder, amplicon_name
- )
- acc_stats = pd.read_csv(acc_stats_file, sep="\t")
- acc_stats_prim = acc_stats.query("Flags in [16, 0]")
- return acc_stats_prim["Acc"].median()
-
-
-def get_reads_usage_stats(folder, amplicon_name, min_size=20, max_size=60):
- cluster_stats_file = "{}/stats/{}_vsearch_cluster_stats.tsv".format(
- folder, amplicon_name
- )
- cluster_stats = pd.read_csv(cluster_stats_file, sep="\t")
-
- min_fwd = min_size / 2.0
- min_rev = min_size / 2.0
-
- total_reads = cluster_stats["n"].sum()
-
- small_clusters = cluster_stats[
- (cluster_stats["n_fwd"] < min_fwd) | (cluster_stats["n_rev"] < min_rev)
- ]
- reads_in_small_clusters = small_clusters["n"].sum()
-
- ok_clusters = cluster_stats[
- (cluster_stats["n_fwd"] >= min_fwd) & (cluster_stats["n_rev"] >= min_rev)
- ]
- reads_in_large_clusters = ok_clusters["n"].sum()
- cluster_count = len(ok_clusters)
-
- excess_reads = sum(
- cluster_stats[(cluster_stats["n"] - max_size) > 0]["n"] - max_size
- )
-
- usable_reads = reads_in_large_clusters - excess_reads
-
- usable_perc = usable_reads * 100.0 / total_reads
- reads_in_small_clusters_perc = reads_in_small_clusters * 100.0 / total_reads
- excess_reads_perc = excess_reads * 100.0 / total_reads
-
- return (
- cluster_count,
- usable_reads,
- total_reads,
- usable_perc,
- reads_in_small_clusters_perc,
- excess_reads_perc,
- )
-
-
-def get_stats(
- folder,
- amplicon_name,
- display_name,
- min_cluster_size,
- max_cluster_size,
- figure_prefix,
-):
- if figure_prefix:
- cluster_read_hist(folder, amplicon_name, display_name, figure_prefix)
- sequenced, mapped, unmapped = get_readstast_bam(folder)
- on_target = count_fastx_reads_on_target(folder, amplicon_name)
- with_umi = count_fastx_reads_with_umi(folder, amplicon_name)
- median_acc = get_median_acc(folder, amplicon_name)
- (
- cluster_count,
- usable_reads,
- total_reads,
- usable_perc,
- reads_in_small_clusters_perc,
- excess_reads_perc,
- ) = get_reads_usage_stats(folder, amplicon_name, min_cluster_size, max_cluster_size)
-
- if total_reads != with_umi:
- print(
- "Warning: clustering numbers don't add up. {} reads missing. This can happen if vsearch excludes UMIs because of length.".format(
- abs(total_reads - with_umi)
- ),
- file=sys.stderr,
- )
-
- return {
- "name": display_name,
- "sequenced": int(sequenced),
- "mapped": int(mapped),
- "on_target": int(on_target),
- "with_umi": int(with_umi),
- "min_size": min_cluster_size,
- "max_size": max_cluster_size,
- "median_acc": float(median_acc),
- "cluster_count": int(cluster_count),
- "usable_reads": int(usable_reads),
- "usable_perc": round(usable_perc, 2),
- "reads_in_small_clusters_perc": round(reads_in_small_clusters_perc, 2),
- "excess_reads_perc": round(excess_reads_perc, 2),
- }
-
-
-def cluster_read_hist(folder, amplicon_name, display_name, figure_prefix):
- filename = "{}/stats/{}_vsearch_cluster_stats.tsv".format(folder, amplicon_name)
- cluster_stats = pd.read_csv(filename, sep="\t")
-
- current_palette = sns.color_palette()
-
- fig = plt.figure(figsize=(15, 8))
- sns.set_style(
- "whitegrid",
- {
- "grid.linestyle": "--",
- },
- )
- sns.set_context("paper", font_scale=3)
-
- tmp = cluster_stats["n"].value_counts().reset_index()
- tmp["Read_count"] = tmp["n"] * tmp["index"]
-
- ax = sns.barplot(x="index", color=current_palette[0], y="Read_count", data=tmp)
- ax.set_xticklabels(ax.get_xticklabels(), rotation=90)
- ax.set(
- xlabel="Cluster size",
- ylabel="Number of reads in clusters",
- title="Cluster size distribution - Sample: {}".format(display_name),
- )
-
- ax.axvline(20, ls="--", zorder=1, label="Min cluster size", color="red", alpha=0.8)
- ax.axvline(60, ls="--", zorder=1, label="Max cluster size", color="red", alpha=0.8)
- ax.set_xlim(0, 200)
-
- xmin, xmax = ax.get_xlim()
- custom_ticks = np.linspace(xmin, xmax, 5, dtype=int)
- ax.set_xticks(custom_ticks)
- ax.set_xticklabels(custom_ticks)
-
- sns.despine()
- plt.tight_layout()
- fig.savefig(str(figure_prefix) + "_cluster_size_distribution.pdf", dpi=300)
- fig.savefig(str(figure_prefix) + "_cluster_size_distribution.png", dpi=100)
- plt.show()
-
-
-def main(argv=sys.argv[1:]):
- args = parse_args(argv=argv)
-
- folders = args.FOLDERS
- figures = args.FIGURES
- name = args.NAME
- c_min = int(args.MIN)
- c_max = int(args.MAX)
-
- df_stats = pd.DataFrame(
- columns=[
- "name",
- "sequenced",
- "mapped",
- "on_target",
- "with_umi",
- "min_size",
- "max_size",
- "median_acc",
- "cluster_count",
- "usable_reads",
- "usable_perc",
- "reads_in_small_clusters_perc",
- "excess_reads_perc",
- ]
- )
- i = 0
- for folder in folders:
- if ":" in folder:
- cols = folder.split(":")
- display_name = cols[0]
- folder = cols[1]
- else:
- display_name = name
-
- figures_ext = None
- if figures:
- figures_ext = "{}_{}_{}".format(figures, name, i)
- stats = get_stats(folder, name, display_name, c_min, c_max, figures_ext)
- df_stats = df_stats.append(stats, ignore_index=True)
-
- i += 1
- print(df_stats.to_csv(sep="\t", index=False, header=True), end="")
-
-
-if __name__ == "__main__":
- main()
diff --git a/config/custom.config b/config/custom.config
index 111c835..1b6d97a 100644
--- a/config/custom.config
+++ b/config/custom.config
@@ -2,53 +2,52 @@
* -------------------------------------------------
* Nextflow config file for custom parameter settings
* -------------------------------------------------
+ * nextflow run umi-pipeline-nf -profile custom
*/
params {
- help = false
- version = false
- debug = true
+ help = false
+ version = false
+ debug = false
- input = "/home/stephanamstler/umi-pipeline_Nextflow/lpa_data/fastq_pass"
- output = "test_nf_pipeline"
- reference = "/home/stephanamstler/umi-pipeline_Nextflow/lpa_data_single_sample/ref/lpa-ref2645.fasta"
- reference_fai = "/home/stephanamstler/umi-pipeline_Nextflow/lpa_data_single_sample/ref/lpa-ref2645.fasta.fai"
- bed = "/home/stephanamstler/umi-pipeline_Nextflow/lpa_data_single_sample/ref/lpa-ref2645.bed"
+ // required parameters
- subsampling = false
- subsampling_readnumber = 30000
+ input = "PATH/TO/fastq_pass/"
+ output = "PATH/TO/OUTPUT_DIR"
+ reference = "PATH/TO/REF.fasta"
+ reference_fai = "PATH/TO/REF.fasta.fai"
+ bed = "PATH/TO/BED.bed"
- write_reports = true
- include_secondary_reads = true
- output_format = "fastq"
+ // adaptable parameters
- filter_strategy_clusters = "quality"
+ output_format = "fastq"
+ filter_strategy_clusters = "quality"
- call_variants = true
- variant_caller = "freebayes"
+ call_variants = true
+ variant_caller = "freebayes"
+ medaka_model = "r1041_e82_400bps_hac_g615"
}
-// NEXTFLOW REPORTING # this defines pipeline metadata
+
+// NEXTFLOW REPORTING
if(params.output != null){
dag {
- overwrite = true
enabled = true
file = "${params.output}/nextflow_stats/dag.mmd"
}
report {
- overwrite = true
enabled = true
file = "${params.output}/nextflow_stats/report.html"
}
timeline {
- overwrite = true
enabled = true
file = "${params.output}/nextflow_stats/timeline.html"
}
trace {
- overwrite = true
enabled = true
+ fields = "task_id, process, tag, status, cpus, time, memory, start, complete, duration, %mem, %cpu, vmem"
file = "${params.output}/nextflow_stats/trace.txt"
}
}
+
diff --git a/config/test.config b/config/test.config
index 5ff85bc..ccfdaa9 100644
--- a/config/test.config
+++ b/config/test.config
@@ -8,26 +8,31 @@
params {
- help = false
- version = false
- debug = true
+ help = false
+ version = false
+ debug = true
- input = "umi-pipeline-nf/data/fastq_pass/"
- output = "umi-pipeline-nf_test-run"
- reference = "umi-pipeline-nf/data/ref/example_egfr_reference.fasta"
- reference_fai = "umi-pipeline-nf/data/ref/example_egfr_reference.fasta.fai"
- bed = "umi-pipeline-nf/data/ref/example_egfr_amplicon.bed"
+ input = "$baseDir/data/fastq_pass/"
+ output = "umi-pipeline-nf_test-run"
+ reference = "$baseDir/data/ref/lpa-ref2645.fasta"
+ reference_fai = "$baseDir/data/ref/lpa-ref2645.fasta.fai"
+ bed = "$baseDir/data/ref/lpa-ref2645.bed"
- write_reports = true
- include_secondary_reads = true
- output_format = "fastq"
+ subsampling = false
- min_reads_per_barcode = 20
+ min_reads_per_cluster = 18
+ max_reads_per_cluster = 20
- call_variants = true
- variant_caller = "freebayes"
+ write_reports = true
+ output_format = "fastq"
+ filter_strategy_clusters = "quality"
+ call_variants = true
+ variant_caller = "freebayes"
+
+ medaka_model = "r1041_e82_400bps_hac_g615"
}
-// NEXTFLOW REPORTING # this defines pipeline metadata
+
+// NEXTFLOW REPORTING
if(params.output != null){
dag {
enabled = true
diff --git a/data/fastq_pass/barcode02/example_egfr_single_cluster.fastq b/data/fastq_pass/barcode02/example_egfr_single_cluster.fastq
deleted file mode 100755
index 3a1fec6..0000000
--- a/data/fastq_pass/barcode02/example_egfr_single_cluster.fastq
+++ /dev/null
@@ -1,200 +0,0 @@
-@f914f21b-12f1-4be6-9aab-c8f4ced363cd runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=135 ch=58 start_time=2020-03-23T16:20:45Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-GTTGTACTTCGTTCCAGTTACGTATTGCTGGTGCACGAAGAAAGTTGTCGGTGTCTTTATTGTTAACCGTATCGTGTAGAGAGACTGCGTAGGTTTAGAAGTTGCATGAGGTTGGGGTTTTAGACCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTGCAAGGGATCTTGAAGGAAACTAAGATTCAAAGATCAAAGTGCTGAGCTCCGGTGCGTTCGGCACGGTGTATAAGTGGGTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGGCTCTCATGGTCTGGTGGGGGAGCCCAGAGTCCTTGCAAGCTGTATATTTCCATCATCTACTTTCTCTTTGTTTCACTGAGTATTTGGGAAACTCAGTGTTTTCCCAAGTTATTGAGAGGAAATCTTTTATAACCACAGTAATCAGTGGTCCTGTGAGACCAATTCACAGACCAAAGGCATTTTTATGAAAGGGGCCATTGACCTTGCCATGGGAGTTGCAGCACAGGGCGGGAGGAGGCCGCCTCTCACCGCACGGCATCAGAATGCAGCCCAGCTGAAATGAAGCCATCTTCGTTTAGCTTCTTCTAGATCCTCTTTGCATGAAATCTGATTTCAGTTGGGCCTAGACGCAGCATCATTAAATTCTGGATGAAATGATCCACACGGACTTTATAACAGGCTTTACAAGCTTGAGATTCTTTTATCTAAATAATCAGTGTGATTCGTGGAGCCCAACAGCTGCAGGGCTGCGAGGGCGTCACAGCCCCAGCAATATCAACCTTAGGTGCGGCTCCACAGCCCCAGTGTCCCCTCACCTTCGGGGTGCATCGCCAGGTAACATCCACCCAGATCACTGGGCAGCATGTAGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCCTTAGGGACTCTGGATCCCAGAAGGTGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAGCAACATCTCCGAAAGCCAACAAGGAAATCCCCTCGATGTGAGTTTCTGCTTTGCTGTGTGGCCAGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGAAACTTCAGCATGAACCGTAAGCCCAAACACTCGCGCTGACTCGATCACTGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCCTTATTACGTAAC
-+
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
-@4519d708-d335-479f-adb3-709364581fdf runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=243 ch=182 start_time=2020-03-23T16:21:31Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CGATGTACTTCGTTCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTATTGTTAACCGTATCGTGTAGAGACTGCATAGGTTTAGAGTTGCGCTTGGGGTTGGGGTTTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAACTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAAGATCAAAGTGCTGAGCTCCGGTGCGTTCGGCACGGTGTATAGGTAAGGTCCCTGGCACAGGCCTCTCTGGAGGCTGGGCCGCAGGGCCCTCTCGCCCGGTCTGGTGGGGAGCCCAGAGTCCTTGCAAGCTGTATATTTCCATCATCTACTTTACTCTTTGTTTCACTGAGTGTTTGGGAAACTCAGTGTTTTCCCAAGTTATTGAGAGGAAATCTTTATAACCACAGTAATCCAGTGGTCCTGTGAGACCAATTCACAGACCAAAGCATTTTGTGAAAGGGGCCATTGACCTTGCCATGGGGTGCAGCACAGGGCGGGAGGAGGGCCGCCTCTCACCACACGGCATCAAGAATGCAGCCCAGCTGAAATGGGCTCATCTTCGTTTGCTTCTTCTAGATCCTCTTTGCATGAAATCTGATTTCAGTTAGGCCCCTGAGCAGCATCATTAAATTCTGGATGAAATGATCCACACGGACTTTATAACGAGCTTTACAAGCTTGAGATTCTTTTATCTAAATAATCAGTGTGATTCGTAGAGGCCCAACAGCTGCTGCCAGGCTGCGGGGGCGTCACAGCCCCCAGCAATATCAGCCTTAGGTGCGGCTCCACAGCCCCAGTGTCCCTCACCTTCGGGGTGCATCGCTGGTAACATCCACCCAGATCACTGGGCAGCATGTAGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCCACAGATCCCAGAAGGTGAAAGTTAAAATTCCGTCGCTATCAAGGAATTAAGAGAAACGTAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGTGGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGAAACTTCAACGCGAACCGTAAACCCAAACACTCGCACTGACTCGATCACTGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCAACAATACGTAGCTTA
-+
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
-@9c516311-61b1-4d43-8224-66fcdb582973 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=320 ch=240 start_time=2020-03-23T16:21:45Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-TGTACTTCGTTTCCGGTTACATTATTTATATTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCAGTAGTCGAGTCAGTGCGAGTGTTTGGGCTTACGGTTCGCGTTGAAGTTTCAGCTGCAGACGCGAGAAAAATTGGGTGAGCCTGAGGTTCAGAGCCATGGACCCCCACACAGCAAAAGCAGAAACTCACATCAGTTTCCTTGTTGGCTTTCGGAGATGTTGCTTCTCTTAATTCCTTGATAGCGACAGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTATGACAGAGAGGAAGACGTTAACAGCAATTGTGAGATGGTGCCACATGCTGCCCAGTGATCTGGAGTGCTTGGATGTTACCAGCGACCACCCCCGAAGGTGAGGACACTGGGGCTGTGGAGCCGCACCTAAGGCTGATATTGCTGAGGCTGTGACCCCCACCCAGCCCTCAGCTGTTGGGCTCCACTGAATCACACCTGTTATTTAGATAAAAGAATCTCAAGCTTGTAAAGCCTGTTATAAAGTCCGTGTGGATCATTTCATCCAGAATTTAATGATGCTGCGTCTGGGTCCTAACTGAAATCAGATTTCATGCAAAGGGATCTAGAAGAAGCAAGCTGAAGATGAGCCCATTTCAGCTGGGCTGCATTCTGATGCCGTGCGGTGAGCGGCCTCCTCCCGCCCTGTGCTGCACCCCATGGCAAGGTCAATGGCCCCTTCATAAAAATGCCTTTGGTCTGTAAGATTGGTCTCACAGGACCACTGATTACTGTGGTTATAAAGATTTCCTCTCAATAACTGGGAAAAACACTGGAGTTTCCCAAACGCTCAGTAGGAAACAAGAGTAAAGTAGATGATGGAAATATACAGCTTGCAAGGACTCTGGGCTCCCCACCCAGACCATGAGGAGCCCTGCGGCCCAACCCAGAGGCCTGTGCCAGGGACCTTACCTTATACACACCGTGCCGAACGCACGGAGCTCAGCACTTTGATCTTTTTGAATTCAGTTTCCTTCCAAGATCCTCAAGAGAGCTTGGTTGAGAACTTCTCCACTGGGTGTGAAGGCTCCAAAGCCCCAACCCCAAGCGCAACTCTAAACCTACGCAGTCTCTACGATACGGTTAACACAAAGACACCGACAACTT
-+
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
-@3033ef91-0437-4df7-b304-a45ce8555294 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=10792 ch=291 start_time=2020-03-23T17:28:52Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CAATGTACTTCGTTCAGTTACGTATTGCTGGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAACCAGTGATCGAGTCAGTGCGAGTGTTTGGGCTTACGGTTCGCGTTGAAGTTTCAGCTGCCAGACATGAGAAAAGGTGGGCCCTGGGTTCGGAAACCATGGACCCCCACACCTTAAACCCGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAGATGTTGCTTCTCTTAATTCCTTATTGCTGACAGGAATTTTAACTTTCTCACCTTCTAGGATCCAGAGTCCTATGACAGAGAGAAGAGGAAGACGTTAACTGGCAATTGTGAGATGGTGCCACATGCTGCCCAGTGATCTGGGTGGATGTTACCAGCGATGCACCCCGAGGTGAGGGACACTGGGGCTGTGGGCCACTAAGGCTGATATTGCTGGGGGCTGTGACGCCCCACAGCCCTGCAGCTGTTGGGCTCACGAATCACTGATTATTTAGATAAAAGAATCTCAAGCTTGTAAAGCCTGTTATAAAGTCCGTGTGGATCATTTTCATCAGAAATTTAATGATGCTGCGTCTAGGCCTAACTAAGGAAATCAGATTTCGTGCAAAGAGGATCTAGAAGAAGCAAACGAAGATGAAGCCCATTTCAGCTGGGCTGCATTCTGATGCCGATGCGGTGAGAGCGGCCCTCACACTCCGCCCTGTGCCACCATAGCTTGAGGTCAATGGCCCCTTTCATAAAAATGCCTTTGGTCTGTGAATTGGTCTCACAGGACCACTGATTACTAATGGTTATAAAAGATTTCCTCTCAATAACTTGGGAAAAAGTACTGGAGTTTCCCCAAACACTCAGTGAAACAAAAGAGTAAAGTAGATGATGGAAATATACAGCTTGCAAGGACTCTGGGCTCCCCACCAGACCATGAGAGGCCTGCGGCCCAGCCCAGAGGCCTGTTGGGACCTTACCTTATACACCGTGCCGAACGCACCGGAGCTCCAGCACTTTGATCTTTTTGGTTCCTTCAAGATCCTCGAAGGCTTGGTTGGGAGCTTCTCCACTGGGTGTAAGAGGCTCCAAAGCCCCAGCCCCAAGCGCAACTCTAAACCTACGCAGTCTCTACACGATACGGTTAACACAAAGACACCGACAACTTTTCTTCCAGCATACAATATTACGTAACT
-+
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
-@90f68bd3-9c59-43f7-bbeb-340bf9d9b06d runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=10975 ch=171 start_time=2020-03-23T17:28:56Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-GTTGTACTTCGTTCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCGTATCGTGTAGAGACTACGTAGGTTTAGAGTTGCGCTTTAGGGTTGAGGTTTGGAGCCTCTCTCTTACACCCAGTGGGCTCCCAAACCAAGCTCCTTGAAAGTCTTGAAATGAATTCAAAAGATCAAAGTGCTGAGCTCCGGTGCGTTCGGCACGGTGTATGGGTAAGGTCCCTGGCACAGGCCTCACAGGCTGGGCCGCGGGGCCTCATGGTCTGGTGAGAGCCCAGAGTCCCTTGCAAACTGTATATTTCCATCATCTACTTACTCTTTGTTTCACTGAGTGTTTGGGAAACTCAGTGTTTTTCCCAAGTTATTTAGAGAGGAAATCTTTTATATACACGGTAATCGGTGGTCCTGTGAGACCAATTCACAGACCAAAGGCATTTTTATGAAGGGGCCATTGACCTTGCCATGGGGTGCAGCACAGGGCAGGAGGAGAACCGCCCTCTCCTCCCACCGGCATCCAGAATGCAGCCCAGCTGAGAATGGGCTCATCTTCGTTTGCTTCTTCGTCTCTTTGCATGAAATCTGATTTCAGTTAGGCCTAGACGCAGCATCATTAAATTCTGGATGAAATGATCCACACGGACTTTATAACGAGCTTTACAAGCTTGAGATTCTTTTATCTAAATAATCAGTGTGATTCGTGGGGAGCCCAACAGCTGCAGGAGCTGCGGGGGCGTCACAGCCCCCAGCAATATCAACCTTGGGGTGCGGCTCCACCTTCAGTGTCCCTCACCTTCGGGGTGCATCGCTGGTAACATCCACCAGATCACTGGGCAGCATGTAGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAGAGGTGAAAGTTAAAATTCCCGTCCTTATCAAGGTGAAGCAACATCTCCGAAGCCAACAAGGAAATCCTCGTGTTGAGTTTCTGCTTTGCTGTGTGGGGGGTCCATGGCTCTGAACCTCAGGTACACTACTCATGTCTGGCAGCTGAAACTTCAACGCGAACCGTAGCCCCAAACACTCGCACTGACTCGTCGCTGGGTTAACACAGACACCGACAACTTTCTTCAGCACCAGCAATACGTAGC
-+
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
-@4c8dcebe-7352-419e-a59c-737ec6269c67 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=9625 ch=483 start_time=2020-03-23T17:29:04Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CAGTGGCCATGGTTCGATTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTGTGTTAACCAGTGATCGATCCAGTGCGAGTGTTTGGGCTTACGGTTCGCGTTGAAGTTTCAGCTGCCAGACATGAGAAAAGGTGGGCCTGAGGTTCGAAACCATGGACTACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAGATGTTGCTTCTCTTAATTCCTTGATAGCGACGGGAATTTTAACTTTCTCACAACTAGGATCCAGAGTCCCTGACAGAGAGAGAAGGAAGACGTTAACTGTAATTGTGGATGGTGCCACATGCTGCCAGTGATCTGGGTGGATGTTACCAGCGATGCACCCCGAAGGTGAGGGACACTGGGGCTGTGGAGCCGCACCCCTAAGGCTGATATTGCTGGGCTGTGACGCCCCCACGCAGCCCCTGCAGCTGTTGGGCTCCACGAATCACACTGATTATTTAGATAAAGAATCTCAAGCATAAAGCCTGTTATAAAGTCCGTGTGGATCATTTCATCCAGAATTTAATGATGCTGCGTCTAGGCCTAACTGAAATCAGATTTCATGCAAAGAGGATCTAGAAGAAGCAAACGAAGATGAGCCCATTTCAGCTGGGCTGCATTCTGATGCCGTGCGGTGAGAGGCGGCCCTCCTCCCCGCCCTGTGCTGCACCCCATGGCAAGGTCAATGGCCCCTTTCCTTAAAAATACTGGTCTGTGAATTGGTCTCTGGGACCACTGATTACTGTGGTTATAAAAGATTTCCTCTCAATAACTTGGAAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAGTAAAGTAGATGATGGAAATATACAGCTTGCAAGGACTCTGGGCTCCCCACCAGACCATGAGGAGCCCCTGCGGCCCAGCCCAGAGGCCTGTGCCAGGGACCTTACACTTATACACCGTGCCGAACGCACCGGGCTCAGCACTTTGATCTTTTTTGAATTCAGTTTTCCCTTCAGATCCTCAAGAGAGCTTGGTTGGGAGCTTCTCCACTGGAGTGTAAGAGGCTCCAAAAACCCCACAACTAAGCGCAACTCTAAACCTACACCAGTCTCTACACGATACGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCAACAACAGCT
-+
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
-@19ff1227-3c72-415f-8ce5-db56e668c0b2 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=9384 ch=51 start_time=2020-03-23T17:29:04Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CAGTGTACTTCGTTCAGTTACGTATTGCTGAATTGCTGAAGAAAGTTGTCGGTTCGATCTTTGTGTTAACCATCGAGTCAGTGCGAGTGTTTGGGCTTACGGTTCGCGTTGAAGTTTCAGCGCAGACATGAGAAAAGGTGAATACCGGGTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAGCTCACATCAGAGATTTCCTTGTTGGCTTTCCGGAGATGTTGCTTTCTTAATTCCTTGAGTAGCGACAGAGATTTTAACTTTCTCACTGTCTGGGATAAAGTCCCTATGACAGAGAGAGAAGGAAGACGTTAACTGGCAATTGTGAGATGGTGCCACATGCTGCCCAGTGATCTGGGTGGATGTTGCAGCGATACCCCGAAGGTGAGGACGCGCGGGGCTGTGGGCCGCACCTAAGGCTGATATTGCTGAGGCTGTGACGCCCCCGCAGCCCTGCAGCTGTTGGGCTCCACGAATCACACTGATTATTTGGATAGTCTCAAGCTTGTAAGCCTGCTATAAAGTCCGTGTGGATCATTTTCATCCAGAATTTAATGATGCTGCGTCTGAGCCTAACTGAAATCAGATTTCATGCAAAGAGGATCTAGAAGAGCAAACGAAGATGAGCCCATTTCAGCTGGGCTGCATTCTGATGCTCCGTGCGGTGAGAGGCGGCCCACTCCTCCCGCCTGTGCTGCGCACCCCATGGCAAGGTCAATGGCCCCTTTCATAAAAATGCCTTTGGTCTGTGAATTGAAGTATCACAGGACCACTGATTACTGTGGTTATAAAGATTTCCTCTCAATAACTTGGGAAAAACACTGGAGTTTCCCAAACACTCCAGTGAAACAAAGAGTAAAGTAGATGATGAAATATACAGCTTGCAAGGACTCTGGGCTCCCCACCCAGACCATGAGAGGCCCTGCGGCCCAGCCCAGAAGGCCTGTGCCAGGGACCTTACCTTATACACCGTGCCGAACGCACCGGAGCTCAGCACTTTGATCTTTGAATTCAGTTTCCCTTCAAGATCCTCAAAGAGCTTGGTTGGGAGCCTCCACTGGGTGTAAAGGCTCCAAAACCCCAACCCCAAGCGCAACTCTAAACCTACACTGATCTCTACGATACGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCAGCAATACGTAACTT
-+
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
-@d300bc8e-98d9-4240-a8da-5bf6f718fba2 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=10434 ch=242 start_time=2020-03-23T17:29:46Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-GATGTACTTCGTTCAGTTACGTATTGCTGGTGCGCTGAAAGTTGTCCGTTGTCTTTGTGTTAACCAGTGATCGAGTCAGTGCGAGTGTTTGGGCTTACGGTTCGCGTTGAAGTTTCCAGCTGCCAGACGCCAGAAAGGTGGGCCTGAGGTTCAGAGGCCATGGACCCCCACACAGCAAAGCGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAGATATTACTTCTCTTAATTCCTTGATAGCGACGGGAATTTTAACTCTCACCTTCTGGGATCCAGAGTCCCTATGACAGAGAGAGGAAGACGTTAACTGGCAATTGTGAGATGGTGCCTTGCTGCCCAGTGATCTGGGTGGATGTTGCCAGCGATGCACCCCGAAGGTGAGGGACACTGGGGCTGTGGAGCCGCACCTAAGGCTGATATTGCTGGGGGCTGTGACGCCCCCGCAGGCCCTGCAGCTGTTGGGCTCCACACAGTCACACTGATTATTTAGATAAAAAAGAATCTTAAGAGCTTGTAAAACTCATTTATGAAGTCCATTATTGGATCATTTCAATCAGAATTTAATGATGCTGCGTCTAGGCCTAACTGAAATCAGATTTCATGCAAAGAGGATCTAGAAGAAGCAAGCAAGATGAGCCATTTCAGCTGGGCTGCATTCTGATGCCGTGCGGTGAGGCGGCCCCTCCTCCGCCCTGTGCTGCACCCCATGTATAGGGTCCAGTGGCCCCTTTCATAAAACACCTTTGGTCTGTGAATTGGTCTCTGGGACCACTGATTACTAATGGTTATAAAAGATTTCCTCTCAATAACTTGGGAAAAACACTGGAGTTTCCCAAACACTCAAGTGAAACAAAGAGTAAAGTAGATGATGGAAATATACAGCTTGCAAGGACTCTGGGCTCCCCCACCAGACCATGAGGCCCTGCGGCCCAGCCCAGAGGCCTGTACCAGGGACCTTACCTTATACACCGTGCCGATGCACCGGGCTCAGCACTTTGATCTTTTTTGAATTCAGTTTCCTTCAAGATCCTCAAGAGAGCTTGGTTGCAGGGCTTCTCCACTTTCAGGTGTAAGAGGCTCCAAAACCCCAACCCCAAGCGCAACTCTAAACCTACGCAGTCTCTACACGATACGGTTAACACAAAGACACCGACAAC
-+
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
-@cc81f15f-6bd4-492d-9a9a-b51be223466d runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=9768 ch=367 start_time=2020-03-23T17:29:45Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CAGTGGCAGCGTTTCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCGTCTGCTCGTGTAGAGACTGCGTAGGTTTAGAGTTGCGCTTGAGGTTGGGGTTTTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGATCTTGAAGGGAAACTGATTAAAAGATCAAAGTGCTGAGCTCCGGGTGCACGTTGGCACGGTGTATAGGTAAGGTCCCTGGCACAGGCCTCTGGGCTGGAGCCACAGGGCCTCATGGTCTGAGGTGGGGAGCCCAGAGTCCTTGCAAAGCTATATTTCCATCATCTACTTTACTCTTTGTTTCACTGAGTGTTGGAAACTCAGTGTTTTCCCAAGTTATTGAAATCTTTTATAACCACAGTAATCAGGTCCTGTGAGACAATTCACAGACCAAAAGGCATTTTTATGAAAGGGGCCATTGACCTTGCAGCGGTGCAGCACAGGGCGGGAGGAGGGCCGCCTCTCCTCACACGGCATCAGAATGCAGCCCAGCTGAAATGAATATCTTCGTTTGCTTCTTCTAGATCCTCTTTGCATGAAATCTGATTTCAGTTAGGCCTAGACGCAGCATCATTAAATTCTGGATGAAATGATCCACACGGACTTTATAACAGGCTTTACAAGCTTGAGGGTTCTTACATCTAAATAATCAGTGTGATTCGTGGAGGCCCAACAGCTGCAGAGTGCGGGGGCGTCACAGCCCCCAGCAATATCAGCCCCTTAGGTGCGGCTCCACAGCCCCAGTGTCCCTCACCTTCGGGGTGCATCGCTGGTAACATCCCACCCAGATCACTGGGCGGCATGTGGCACCATCCTCACAGTGCCAGTTAACGTCTTCCTTCTCTCTCATGTCATAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCCTTGTGGGGTCCATGGCTCTGAACCTCAGAACCCACCTTTTCTCATGTCTGGCAGCTGAAACTTCAACGCGAACCGCGTAAGCCCAAACACTCGCTGACTCGATCACTGGTTAACACAAAGACACCGACAACTTTCTTTGGCATAAGCAATATAGCAAC
-+
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
-@013dc296-9434-43c4-9975-7e994052f1c1 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=7402 ch=387 start_time=2020-03-23T17:30:22Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CGGTATGCTTCGTTCAGTTACGTATTGCTGAAAGAAGTTGTCGGTGTCTTTGTGTTAACCAGTGATCGAGTCAGTGCGAGTGTTTGGGCTTACGGTTCGCGTTGAAGTTTCAGCTGCCAGACATGAGAAAAGGTGGGCCCTGAGGTTCAAGCCATGGACCCCCACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAGATGTTGCTTCTCTTAATTCCTTGATAGCGACGGGAATTTTAGCTTTCTCACCTTCACGGATCCCAAAGAGTCCCTATGACAGAGAGAGAAGGAAGACGTTAACTGGCAATTGTGAGATGGTGCCACATGCTGCCCAGTGATCTGGGTGGATGTTACCAACGTGCACCCCGAGGTGAGGGACACTGGGGCTGTGGAGCCGCACCTAAGGCTGATATTGCTGGGGGCTGTGACGCCCCGCAGCCCTGCAGCTGTTGGGCTCCACGAATCACACTGATTATTTAGATAAAAAGAATCTCAAGCTTGTAAAGCCTGTTATAAAGTCCGTGTGGATCATTTCATGTAAAATTTAATGATGCTGCGTCTAGGCCTAACTGAAATCAGATTTCATGCAAAGAGGATCTAGAAGAAGCAAACGAAGATGAGCCCATTTCAGCTGGGCTGCATTCTGATGCCGTGCTGAGGAAGCTGGCCCTCCTCCCGCCCTGTGCTGCACCCATGGCAAGGTCAATGGCCCCTTTCATAAAAATGCCTTTGGTCTGTAGTTGGTCTCACCCAGGACCCTGTGGTGCTATGGTTATAAAAGATTTCCTCTCAATAACTTAGGAAAAACACTGGGAGTTCCAAACACTCAGTGAAACAAAAGAGTAAAGTAGATGATGAAATAGCACAGCTACAGGGCTCTGAAACCCCACCCAGACCATGAGAGGCCCTGCGGCCCAGCCAGAGGCCTGTGCCAGGGACCTTACCTTATACACCGTGCCGAACGCACGGAGCTCAGCACTTTGATCTTTTTGAATTCAGTTTCCTTCAAGATCCTCGAAGGCTTGGTTTAGGGAGCTTCTCCACTGGGTGTAAGAGGCTCCAAACCCCAACCCCAAGCGCAACTCTAAACCTACGCAGTCTCTACACGATACGGTTAACACAAAGAAGACACCGACAACTTTCTTCAGCACCAGCAATACGTAACT
-+
-%$&(%'(1398;D-828=;0B>BBFGAAA=<2&$$$BDF73A@F>?$)9>D86@ADEEH0$(*$$49:.6(1&2=8.+(<;:A>6<1:>80AC@JD23<5*01+$)&7(,;>;9@A1;=J/1*-(B??F889;..++.3506@A>9,;,;=C@CB4B;9;228:7HKK464@<;<=@?CD,4)/8<@:1C>-49535939CDBD>@CKHF8A40:.?&->A<>>>.?$&&*:8<@><<94&$%(.5$$'$%'%#'59:@@A<23)00)?;720*15?BB>C?9:ADBGCBG?B>?A>?B>;A@A=@;=<@C@C@IHDEBHA>:C?61@??BB@>969<7)*)$@=E=FGF;:-)1(78=GB@??@CLEDFFD@A(?=9799B><780=>934:88=?;>67:;800-08/133686*..0;:5/4=9DCDGEF3/:=;%-033-16?99C@;A?=5*/>?:;5?56;99:9=BDC9***06;9@DHD>@BAAIB:::;/(=-3,-8%69/&.//@7.=8?:FGB4'-%/02%81<?=?5B8C?DA96+8**$../)$$'%#&&889878<:=<5?@F=?B>=D9@655:$H8?@569-)=?B?:7433--.112$(BA6900<14,&=<=:=BDBD@C<541(&)-%,+.)$');?>@:9A@@DI;;+=%-.(%"%$$%0/)$('&)$&(&')&%$*.:99;BCBED@;530*-**:447*4929CC>?7668025?@=@;>,+++3;>:6;6958:9>?BEE=?=:H??:211,$'$%$($%;+06*:<AD9,(4@:-945:B?.:<:=8>=?87..-***35(./2)A>>=33:488357;23;;B6?2LO7CEJ.:2;99=@GEF9<>G7:6<<;<;C@>?<9BC%%$$&1115315:...4:B@G8>111174@.7>?A?@CE@<..
-@3d1d93fe-7292-493f-98d5-38170e5f77ca runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=11160 ch=171 start_time=2020-03-23T17:30:41Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-GTTACCCACTTCGTTCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTATTTAACCGTATCATTGTAGAGACTGCGTAGGTTTAGAGTTGCGCTTGGGGTTGGGGTTTGGAGCCTCCGCAGTGGAAGCTCCTTAAGCTCTCGAGGATCTTGAAGGAAACTGAATTCAAAAGATCAAAGTGCTGAGCTCAGTGCGTTCGGCACGGTGCAAGGTAAGTTCCCTGGCACAGGCCCTCTGGGCTGGGCCGCAGAACTCTCATGGTCTGGTGGGAGCCTTGGAGTCGCGCCCCGCAAGCTGTATATTTCCATCATCTACTTTACTCTTTGTTCACTGGTGTTTAAGGCTTTCAGTGTTTCCCAAGTTATTGAGAGGAAATCTTTTATAACCACAGTAATCAGTGGTCCTGTGAGACCAATTCACAGACCAAAGGCATTTTTATGAAGGGGCCATTGACCTTGCCATGGGGGTGCAGCACGGGGCAGGAGGAGGGCCACCCTCTCACCACGGCATCAGAATGCAGCCCAGCTGAAATGGGCTCATCTTCGTTACACTTCTTCTAGATCCTCTTTGCATGAAATCTGATTTCAGTTGAGCTGGGCACAACATCATTAAATTCTGGATGAAATGATCCACACGGACTTTATAACAGGCTTTGAAGCTTGAGGTTCTTTTATCTAAATAATCAGTGTGATTTCGTAGGACCCAACAACTGCAGGGCTGCAGGGACGTCACAGCCCCCAGCAATATCAGCCTTAGGTGCAGCTCCACAGCCCCCAGTGTCCCTCACCTTCAGGGGTGCATCATGGTAACATCCACCCAGATCACTGGGCAGCATGACACCATCTCACAATTGCCCAGTTAACGTCTTCCTTCTCTGTCATAGGGACTCTGGATCCCAGAAGGTGAGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGTGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGAAACTTCAACACCGAACCGTAAGCCCAAACACTCACTGACTCGATCACTGGTTAACACAAAGAAGACACCGACAACTTTCTTCAGCGACAATACGTAATA
-+
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
-@b5571b18-6335-4b5f-8fe2-7d7ba5b66e1f runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=10625 ch=162 start_time=2020-03-23T17:31:06Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-GATGTACTTCGTTCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCGTATCGTGTAGAGACTGCGTAGGGTTTGGGGAGTTGCGCTTGGGGTTGGGGTTTTGGACTCTTACACCCAGTGGAGAAGCTCCCAACCAAACTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAAAGATCAAAGTGCTGAGCTCCGGTGCGTTCGGCACGGTGTATAGGTAAGGTCCCTGGCACAGGCCTCTGGGGCTGGGCCGCAGGGCCTCATGAGGGTCTGGTGGGAGCCCAGAGTCCTTGCAAGCTGTATATTTCCATCATCTACTTTACTCTTTGTTTCACTGAGTGTTTGGGAAACTCAGTGTTTTTCCCAAGTTATTGAGGAGGAAATCTTTTATAACCACAGTAATCAGTGGTCCTGTGAGACCAATTCACAGACCAAAGAGCATTTTTATGGGGGCCATTGACCTTGCCATGGGGTGCAACACAGGGCGGGAGGAGGGAGCCGCCTCTCACCGCACGGCATCAGAATGCAGCCCAGCTGAAATGGGCTCATCTTCGTTTGCTTCTTCTGATCCTCTTTGCATGAAATCTGATTTCCAGTTGGCCTAGACGCAGCATCATTAAATTCTGGGTAAATGAAAGTCCACACCCGGACTTTATAACAGGCTTTACAAGCTTGAGATTCGCATATCTAATAATCAATTGTGATTCGTGGAGCCCAACAGCTGCAGGGCTGCGGGGCGTCACAGCCCCCAGCAATATCAGCCTTAGGTGCGGCTCCACAGCCCCAGTGTCCCTCACCCACTGGGAGTTGCATCATTTGGTAACATCCACCCAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAATAACAAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGTGAGAGTCCATGGCTCTGAACCCCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGAAACTTCAACGCGAACCGTAAGCCCAAACACTCGCACTGACTCGATGCTGTTACTTACAAAGGCCACCGACAACTTTCTTCAACATAGCAATACGTAAC
-+
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
-@7c1d5f3d-238c-43d5-8921-75d7f23d4a9e runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=10948 ch=369 start_time=2020-03-23T17:31:14Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-GTTGTACTTCGTTCGGTTACGTATTGCTGGTTGCTGAAGAAAGTTGTCGGTGTCTTTATTGTTAACCGTATCGTGTAGAGACTGCGTAGGTTTAGAGTTGCGCTTGGGGTTGGGGTTTTGGAGCCTCTTACACCCAGTGGGAAGCTCCCCAACCAAGCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAGATCAAAGTGCTGAGCTCCGGTGCGTTCGGCACGGTGTATAAGGTAAGGTCCTGGCGCAGGCCTCTGGGCTGGGCCGCAGGGCTATCATGGTCTGGTGGGGAGCCCAGAGTCCTTGCAAAGCTGTATATTTCCATCATCTACTTTACTCTTTGTTTCACTGAGTGTTTGGGAAACTCCAGTGTTTTTTCCCAAGTTATTGAGAGAAATCTTTTATAACCACAGTAATCAAGTGGTCCTGTGAGACCAATTCACAGACCAAAGGCATTTTTATGAAAGGGGCCAGTGACCTTGCCATGGGGTGCAGCACAGGGCGGGAGGGCCGCCTCTCCTGCACGGCATCAGAATGCAGCCCAGCTGAAATGGGCTCATCTTCGTTTCAGCTTCTTCGAATCCTCTTTGCATGAAATCTGATTTCCAGTTAGGCCTAGACGCAGCATCAGTATTAAATTCTGGATGAAGAATGATCCACACACGGACTTTATAACAGGCTTTACAAGCTTGAGATTCTTTTATCTAAATAATCAGTGTGATTCGTGGAGCCCAACAGCTGCAGGGGCTGCGGGGCGTCACAGCTAGCAATATCAGCCTTAGGTGCGGCTCCACAGCCCCAGTGTCCCTCACCTTCGGGGTGCATCGCTGGTAACATCACAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGGTGAAAGTTAAAATTCCCACGTCGCTATCAAGGAATTAAGAGAAACAACATCTCCGAAAGCCAACAAGGAAACCTCGATGTGGTTCTGCTTTGCTGTGTGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGAAACTTCAACGCGAACCGTAAGCCCAAACACTCGCACTGGCTTCGATCACTGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCAGCAATACGTAAC
-+
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
-@23c037b8-cd60-4748-9e62-e65ba453c88f runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=10587 ch=299 start_time=2020-03-23T17:31:28Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-GTTGTACTTCGTTCGGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCGTATCGTGTAGAACTGCGTAGAGTTTAGAGTTGCGCTTGGGTTGAGTTTTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGATCTTGAAGGAAACTGAATTCAAAAAGATCAAGTGTGACTCGGTGCGTTCGGCACGGTGTGGAAATAGGTCCTGGCACAGGCCTCTGGGCTGGGCCCGCAGGGCTCTCATGGTCTGGTGGGAGCCCAGAGTCCTTGCAAGCTGTATATTTCCATCATCTACTTTACTCTTTGTTTCACTGAGTGTTTGGGAAACTCCAGTGTTTTCCCAAGTTATTGAGAGGAAATCTTTTATAACCACAGCAATCAAGTGGTCCTGTGAGACCAATTCACAGACCAAAGGCATTGCACATGAAAGGGCCATTGACCTTGCCATAGGGTGCAGCACAGGGCGGGAGGAGGGGAGCCGCCTCTCCTCACGGCATCAGAATGCAGCCCAGCTGAAATGGGCTCATCTTCGTTTAGCTTCTTCTAGATCCTCTTTGCATGAAATCTGATTTCAGTTAGGCCTAGACGCAGCATCATTAAATTCTGGATGAAATGATCCACGGACTTTATAACAGGCTTTACAAGCTTGAGGTTCTTTTATCTAAATAATCAGTGTGATTCATTGGAGCCCAACAGCTGCAGGGCTGCGGGGGCGTCACAGCCCCAGCAATATCAGCCTTAGGTGCGGCTCCACAGCCCCAGTGTCCTCACCTTCCAGGGTGCATCGCTGGTAACATCCACCCAGATCACTGGGCAGCATGTGGCACCATCTCCACAATTGCTGATTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGAAATCCTCGATGTGAGTTTCTGCTTTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTTCTCATGTCTGGCAGCTGAAACTTCAACGCGAACCATAAGCCCAAACACTCGCACTGACTCGATCACTGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCGGCAATACGTAAC
-+
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
-@f5c539bd-b104-40d5-89b9-8b5c769399c0 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=9874 ch=399 start_time=2020-03-23T17:31:44Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CAGTACTTCGTTCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCGTATCGTGTGAACTGCGTAGGTTTAGAGTTGCGCTTGGGGTTGGGGTTTTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAATCTTGAAGGGAAACTGAATTCAAAGATCAAAGTGCTGAGCTCCGGTGCGTTCGGCACGGTGTATAAGGTAAGGTCCTGGCACAGGCCTCTGGGCTGGGCCACCGGGCCTCTCATGGTCTGGTGGGGAGCCCAGAGTCCGCCTAGCTGTATTTCCATCATCTACTTTACTCTTTGTTTCACTGAGTGTTTGGGAAACTCCAGTGTTTTCCCAAGTTATTGAGAGGAAATCTTTTATAACCACAGTAATCAGTGGTCCCTGTGAGACCAATTCACAGACCAAAGGCATTTTATGAAAGGGCCATTGACCTTGCCATGGGGTGCAGCACAGGGGCGGGGAGGAGAACCGCCTCTCACCGCACGGCATCAGAATGCAGCCCAGCTGAAATGGGCTCATCTTCGTTTGCTTCTTCTAGATCCTCTTTGCATGAAATCTGATTTCCAGTTAGGCCTAGACGCAGCATCATTAAATTCTGGATGAAATGATCCACACGGACTTTATAACAGGCTTTACAAGCTTGAGATTCTTTTATCTAAATAATCAGTGTGATTCGTGGAGGCCCAACGGCTGCAGGGCTGCGGGGCGTCACAGCCCCCGCAATATCAGCCTTAGGTGCGGCTCCACCCAGCCCCAGTGTCCTCACCTTTGGGGTGCATCGCTGGTAACATCCTGCCAGATCACTGGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCCTTTGCTGTGTGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTTCTCATGTCTGGCAGCTGAAACTTCAACGCGAACCGTGGCCAAACACTCGCACTGACTCGATCACTGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCAGCAACTTCATAACT
-+
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-@8766f35f-096d-422b-af1e-8eaf679f6d65 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=8958 ch=454 start_time=2020-03-23T17:32:21Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-GTTGTACTTCCCGTTCAGTTACATGTATAATTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCGTACATCCATCTTATATAGAACATAGGTTTAGAGTTGCGCTTGGGGTTGGGGTTTTGGAGCCTCACACCCAAGTGGAAGCTCACAACAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAAAGATCAAAGTGCGCTACAGGCTCGGTGCGTTCGGCACGATTGTAAGGTAAGGTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGGTGGGGGCCAGAGTCCTTGCAAGCTGTATATTTCCATCATCTACTTTACTCTTTGTTTCACTGAGTGTTTGGGGAAACTCAGTGTTTTTCCCAAGTTATTGAGAGGAAATCTTTATAACCACGGTAATCAGTGGTCCTGTGAGACCAATTCACAGACCAAAGGCATTTTTATGAAAGGGGCCATTGGCCTTGCCATGGGGTGCAGCACAGGGCGGGAGGAGGGCCGCCTCTCCTGCACGGCATCAGAATGCAGCCCAGCTGAAATGGGCTCATCTTCGTTTGCTCTTCTAGATCCTCTTCACCTTGAAATCTGATTTCAGTTAGGCCTAGACGCAGCATCATTAAATTCTGGATGAAATGATCCACACGGACTTTATAACAGGCTTTACAAGCTTGAGATTCTTTTATCTAAATAATCGGTGTGATTCGTGGAGCCCAACAGCTGCGGGGAGCTGCAGGGGGGCGTCTGGCTAACAATATCAGCCTTAGGTGCGGCTCCACAGCTAGTGTCCTCACCTTCGGGGTGCATCGCTGGTAACATCCACCCAGATCCACTGGGCAGCGTAACTAACATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCGCGGGACTCTGGATCCCAGAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAAGGAATTGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGGTTTCTGCTTTGCTGTGTGAGGGTCCTTGGCTCTGAACCTCAGGCCCGCAGCACTTTCTCATGTCTAGCGGCTGAAACTTCAACGCGAACCGTAAGCCCAAACACTCGCACTGACTCGATCACTGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCAGCAATACGTAAACA
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-@ed75680d-abc3-4cb6-aca2-fb78365839ae runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=9071 ch=351 start_time=2020-03-23T17:33:17Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-TGTTGTACTTCGTTCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCAGTGATCGAGTCAGTGCGAGTGTTTGGGCTTACGGTTCGCGTTGAAGTTTGACTGCCAGACATGAGAAAGGTGGGCCTGAGTTCAGAGCCATGGACCCCACACAGCAAAGCAGAAACTCATCGAGGATTTCCTTGTTGGCTTTCGGAGATGTTGCTTCTCTTAATTCCTTATTAGCAGAACTGAATTTTTAACTTTCTCACCTTCTGGGATCCAGAAGTCCCTATGACGAGAGAAGGAAGACGTTAACTTTGGCAATTGTGAGATGGTACCACATGCTGCCAAATGATCTGGGTGGATGTTACCAGCGATGCACCCCGAAGGTGAGGGACACTGGGGCTGTGGAGCCGCACCTAAGGCTGTTGCTGGGGGCGTAAAGCGCCCCGCAGCCCTGCAGCTGTTGGGCTCCACGAATCACACTGATTATTTAGATAAAAGAGTCTCAAGCTTGTAAAGCCTGTTATAAAGTCCGTGTGGATCATTTCATCAGGAATTTAATGATGCTGCGTCCTGACCTAACTGAAATCAGATTTCATGCAAGAGGATCTAGAAGCAAACGAAGATGAGCCCATTTCAGCTGGGCTGCATTCTGATGCCGTGCGGTGAGAGGCGGCCCTCCTCCCGCCCTGTGCTGCACCCCGCAGGTCAATGGCCCCTTTCATAAAAATGCCTTTGGTCTGTGAATTGGTCTCACAGGACCACTGATTACTGTGGTTATAAGATTTCTCTCAATAACTTGGGAAAAACACTGGAGTTTCCCAAACACTCCAGTGAAACAAAGAGTAAAGTAGATGATGGAAATATACGGCTTGCAAGGACTCTGGGCTCCTACCCAGACCATGAGAGGCCCCTGCAGCTGGCCCAGAAGGCCTGTGCCAGGGGCCTTACCTTATACACCGTGCCGAACGCACCCGGAGCTCAGCACTTTGATCTTTTTGAATTCAGTTTCCTTCAAGATCCTCAAAGAGAGCTTGGTTGGGAGCTCTCCCACTGGGTGTAAGGAAACAAGAGCCCCAACCCCAAGCGCAACTCTAAACCTACGCAGTCTCTACACGATACGGTTAACACAAAGACACCGACAACTTTCTAGCAATACGTAGC
-+
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
-@cdb917f3-05c3-46ad-818b-fdfc43ce2285 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=9733 ch=59 start_time=2020-03-23T17:33:22Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CACATGCTTCGTTCAGTTACACGTATTACTGGTGCTGAAAGTTGTCGGTGTCTTTATTGTTAACCGTATCGTGTAGAGACTGCGTAGGTTTCACGTTACAGTGCCATTGCGGTTTTGGAGCCTCTGCCGCAGTGGAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAAGATCCAAAGTGCTGAGCTCCGGTGCGTTCGGCACGGTGTATAAGGTAAGGTCCCTGGCACAGGCCTCTGGGCTGGAGCCGCAGGGGCCTCTCATGGTCTGGTGGGGAGCCCAGAGTCCTTGCAAGCTGCTATATTTCCATCATCTACTTTACTCTTTGTTTCACTGAGTGTTTAGGAAACTCAGTGTTTTCCCAAGTTATTGAAGGAAATCTTTTATAACCACAGTAATCAGTGGTCCTGTGAGACCAATTCACAGACAGGCATTTTTTTATAGAAAGGGGCAGTGACCTTGCCATAGAGGGTGCAGCACAGGGCGGGAGGAAACCGCCTCTCACCACACGGCATCAGAATGCAGCCCGCTGAAATGGGCTCATCTTCGTTTGCTTCTTCTAGATCCTCTTTGCATGAAATCTGATTTCCAGTTAGGCCTAGACGCAGCATCATTAAATTCTGGATGAAATGATCTACGGACTTTATAACAGGCTTTACAAGCAGGAATTCTTTTATCTAAATAATCAGTGTGATTCGTAAGGCTACCCAACAGCTGCACGGGGCTGCGGGGGCGTCACAGCCCCAGCAATATCAGCCTTAGGTGCGGCTCCACAGCCCCAGTGTCCCTCACCTTCGGGTGCATCGCTGGTAACATCCCACCCAGATCACTGCTGGGCGGCATGTAACACATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCTTAGGACTCTGGATCCCAGAAGGTGAAAGTTAAAATTCCACGTCGCTATAAGGAATTGAAGAAGCAACATGCTCGAAAGCCAACAAGGAAATCCCCCTCGTGTAGAGGTTTCCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGAAACTTCAACGCGAACCGTAAGCCCAAACACTCACTGACTCGATCACTAGTTAACACAAAGACACCGACAACACCTTCCAGCACCAGCAATACGTAAC
-+
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-@d3d771c8-1691-4841-906b-93f96c74b07a runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=10202 ch=279 start_time=2020-03-23T17:34:12Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-GTTGTATTACTTCGTTCAGTTACACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTGCCAATGTTAACCGGTGATCGAGTCAGTGCAGTGTTTGGGCTTACGGTTCGCGTTGAAGTTTCAGCTGCCAGACATGAAAAGGTGGGCCTGAGGTTCAGAGCCATGGACCCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAGATGTTGCTTCTCTTAATTCCTTGATGTGACGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTATGACAGAGAGAAGGAGAAGACGTTAACTGGCAATTGTGAGATGGTGCCACATGCTACTTACAGTGATCTGGGTGGATATTTTCCAACGATATTTAGAAAGGTGGGGACACTGGGGCTGTGGAGCCGCACCTAAGGCTGATATTGCTGAGCTGGCCTTACAGCCCTGCAGCTGTTGGGCTCCACGAATCACACTGATTATTTAGATAAAAGAATCTCAAGCTTGTAGAAGCCTGTTATAAAGTCCGTGGATCATTTCATCCAGAAGATTTAATGATGCTGCGTCTAGGCCTAACTGAAATCAGATTTCATGCAAGAGGATCTAGAAGAAACAAGCAGATGAAGCCCATTTCAGCTGGGCTGCATTCTGATGCCGTGCGGTGAGAGGCGGCCCTCCTCCCCGCCTGTGCTGCACCCCATGGCAGGTCAATGGCCCCTTTCATATACAAAAATGCCTTTTGGTCTGTGAATTGGTCTCACAGGACCACTGATTACTGTGGTTATAAAGATTTCCTCTCAATAACTTGGGAAAAACACTGGAGTTTCCCTCAGTGAAACAAAGAGTAAAGTAGATGATCAAATATACAGCGCTTGCAAGGACTCTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCCAGCAGAGGCCTGTGCCAGGGACCTTACACTATGCACCGTGCCAGGAACGCACCCGGAGCTCAGCACTTTGATCTTTTTGAATTCAGTTTCCTTCAGATCCTCAAAGAGAGCTTGGTTGGGAGCTTCTCCACTGGGTGTAAGAGGCTCCAAAACCCCAACCCCAAAGCGCAACTCTAAACCTACGCAGTCTCTACACAGAAATACTGGTTAACACCAAAGAACACCGACAACTTTCTTCAGCACCAGCAATACGTAAC
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-@a89f31ac-c69e-4526-a10e-d63974e0f409 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=1830 ch=392 start_time=2020-03-23T16:29:50Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CGTACTTCGTTCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTTGTGTTAACCGTATCGTGTAGAACTGCGTAGGTTTAGAGTTGCGCTTGGGGTTGGGTTTTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCCAACCAAGCTCTCTTGAGATCTTGAAGGAGCTGAATTCAAAAAGATCAAAGTGCTGAGCTCCGGTGCGTTCGGCACGGTGTATAAAAGGTAAGGTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGGTGGGGGAGCCCAGAGTCCTTGCAAACTGTATATTTCCATCATCTACTTTACTCTTTGTTTCACTGAGTGTTTGGGAAACTCCAGTGTTTTTCCCAAGTTATTGAGAGGGAAATCTTTTATAACCACAGTAATCAGTGGTCCTGTGAGACCAATTCACAGGCCAAGGCATTTTTATGAAAGGGGCCATTGACCTTGCCATGGGGTGCAGCACAGGGCGGGAGGAGGCCGCCTCTCACCGCACGGCATCAGAATGCAGCCCAGCTGAAATGGGCTCATCTTCGTTTGCTTCTTCTAGATCCTCTTTGCATGAAATCTGATTTCAGTTAGGCCTAGACGCAGCATCATTAAATTCTGGATGAAATGATCCACACGGACTTTATAACAGGCTTTACAAGCGGGATTCTTTTATCTAAATAATCCGGTGTGATTCGTGGAGCCCAACAGCTGCAGGGCTGCGGGGCGTCACAGCCCCCAGCAATATCCAGCCTTAGGTGCGGCTCCACAGCCCCAGTGTCCCTCACCTTCGGGGTGCATCGCTGATAACATCCACCCAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAAGCAACAGCTCGAAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGTGAAGAGTCCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGAAACTTCAACGCGAACCACCGTAAGCCCAAACACTCATACACTGACTCGATCACTGGTTAACACTACGAGACACCGACAACTTTCTTCAGCACCAGCAATACGTAAC
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-@8c389264-fc06-48a3-a899-f2fd15905421 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=11637 ch=314 start_time=2020-03-23T17:34:54Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CAGTGTACTTCGTTCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCAGTGATCGAGTCAGTGCGAGTGTTTGGGCTTACGGTTCGCGTTGAAGTTTCAGCTGCCAGACATGAGAAAGGGTGGGCCTGAGGTTCAGAGCCATGGACCCCCACACAGCAAAGCGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAGATGTTGCTTCTCTTAATTCCTTGATAGCGACGAATTTAACTTTCTCACCTTCTGGGATCCAGGAGTCCCTATGACAGAGAGAGAAGGAAGACGTTAACTGGCAATTGTGAGATGGTGCCACATGCTGCCCAGTGATCTGGGTGGATGTTACCAGCGATGCACCCCGAAGGTGAGGGGACACTGGGGCGTGGGCGCACCTAAGGCTGATATTGCTGGAAACAGCGAGCGCCCCCGCAGCCTCGCTGTTGGGCTCCACGAATCACACTGATTATTTAGATAAAAGAATCTCAAGCTTGTAAAGCCTGTTATAAGTCCCGTGTGGATCATTTCATCAGGAATTTAATGATGCTGCGTCTAGGCCTAACTGAAATCAGATTTCATGCAAAGAGGATCTAGAAGAAGCAAACGAAGATATGAGCCCATTTCAGCTGGGCTGCATTCTGATGCCGTGCGGTGAGAGGCGGCCCTCCTCCGCCCTGTGCTGCACTTAGCAGGTCAATGGCCCCTTTCGCCAAAATGCCTTTGGTCTGTGAATTGGTCTCACAGGACCACTGATTACTGGCATAAAGATTTCCCTCTCAATAACTTGGGAAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAGAGTAAAGTAGATGATGGAAATATACAGCTTGCAAGGACTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCCAGCCCAGAGGCCTGTGCCAGGGACCCATACATATACACCGTGCCGAACGCACCGGAGCTCAACACTTTGATCTTTTTGAATTCAGTTTCCTTCAAGATCCTCAAGAGAAGCTTGGTTGGGAGCTTCTCCACTGGGTGTAAGAGGCTCCAAAACCCCAACCCCAAGCGCAACTCTAAACCTACACCAGTCTCTACACGACACGGTTAACACAAAGACACCATTAACTTTCTTCAGCACCAGCAATAAGC
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-@030968cb-7cd7-4419-bf6d-c40d3463efe6 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=7296 ch=452 start_time=2020-03-23T17:35:41Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CGATGGCGCGTTCAGTTGTGTGCTGGTGCTGAAAGAAAGTTGTCGGTGTCTTTGTGTTAACCAGTGATCGAGTCAGTGCGAGTGTTGGGCTTACGGTTCGCGTTGAAGTTTCAGCTACCAGACATGAAAAGGTGGGCCTGAGGTTCAGAGCAAATGGACCCACACAGCAAAGCAGAAACTCACATCGAAGTTTCCCTTGTTGGCTTTCCGGAGATGTTGCTTCTCATAATTCCTTGATAGCGACGAGATTTTAACTTTCTCACCTTCATAAGGATCAGAGTCCCCTATGATAAGAGAAGGAAGACGTTAACTAGCAATTGTGAGATGGTGCCACATGCTGCCCAGTGATCTGGGTACGGATGTTACCAGCGATGCACCCAGGTGAGGGACACTGGGGCTGTGGAGCCGCACCTAAGGCTGATATTGCTGGGGGCTGTGACGCCCCACCGCAGCCCCTGCAGCTGTTGGGCTCCACGAATCCACACTGATTATTTGAATAAAAGGAATCTCAAGCTTGTAAAGCCTGTTATAAAGTCCATTGTGGATCATTTCATCAGAATTTAATGATGCTCGTCTAGGCCTAACTTGAAATCAGATTTCATACAAGAAAGGATCTAGAAGAAGCAAGCAGATGAGCCCATTTCAGCTGGGCTGCATTCTGATGCCGTGCGGTGAGGGCGGCCTCCTCCCCGCCACATGCTGCACCATGGCAAAGGTCAATGGCCCCTTTCATAAAAATACCTTTGGTCTGTAGATTGATGCTCCTACAGGACCACTGATTACTAATGGTTATAAAAATTTCCTCTCAATAACTTGGGAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAGAGTCGTAGATGATGAAATATATACAGCTTGCAAGGACTCTGGGCTCCCACGCAGACCATGAGAGGCCCTGCGGCCCAGCCAGAGGCCTGTGCCGGGGACACCACGCCACGCAAATTGCCGAGACGCACCCGGAGCTCAGCACTTTGATCTTTTAATTCAGTTTCCTTCAAGATCCTCAAGGAGCTTAGTTTGGGAGCTTCTCCACTGGTGTAAGAGGCTCCAAAACCCCAACCCCAAGCGCAACTCTAAACCTACGCAGTCTCTACACGATACGGTTAACACAAAGACACCGACAACTTTCTTCCAGCACCAGCAATACATAACTT
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-@ce5afae8-456f-4c7d-a10b-acdd18a54e28 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=12021 ch=448 start_time=2020-03-23T17:37:03Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-GATGTACTTCGTTCGAGTTTGTGTTGCTGGTGCTGAAGGAGGTTTGTCGGTGTCTTTGTGTTAACCAGTGATCGAGTCAGTGCGAAGTGTTTGGGCTTACGGTTCGCGTTGAAGTTTCAGCTGCCAGACGCAAAAAGGTGGGCCTGAGGTTCAGAGCCACATGGATTCCACACAGCAAAAGCAGAAACTCACATCGAGTTTCCTTGTTGGCTTTCGGAGATGTTGCTTCTCTTAATTCCTTGATAGCGACGGGAATTTGCTTTCTCACCTTCTGGGGTCAGGTGCTATGACAGAAGGAAGGCAGAGATTAAACCTGGCAATTGTGAGATGGTGCCGCATGCTGCCCAGTGATCTGGAGTGGATGTTACCAGCGATGCACCCCGGGTGAGGGACACTGGGGCTGTGGAGCCGCACCTAAGGCTGATATTGCTGGGGCTGTGACACCCGCAGCCCTGCTTCTGGCTGTTGGGCTCCACGAATCACATGACCCCAGATAAAAGAGATCTCAGAACATGAAAGCCTGTTATAAAGTCCCGTGTGGATCATTTCATCAGAATTTAATGATGCTGCGTCTAGGCTAACTGAAATCAGATTCATGCAAAGAGGATCTAGAAGAAGCAAACGAAGATGAGCCCATTTCAGCTGGGCTGCATTCTGATGCCGTGCGGTGAGAAGGCGGCCCCTCCTCCCGCCTGTGCTGCACCCCATGGCAGGTCAATGGCCCCTTTCATAAAAATGCCTTTGGTCTGTGGTGCGGTCTCACAGGACCACTGATTACTGTGGTTATAAAGATTTCCTCCTCAATAACCACTGGGAAAAACTGGAGTTTTCTAACACTCAGTGAACAAGAGTAAAGTAGATGAGTGGAAATATACAGCTTGCAAGGACTCTGGGCTCCCCACCCAGACCATGAGGGTACTTTGGGGGGCGAAAGCAGAGGCCTGTGCCAGGGACACATATACACCGTGCCAGAACACACAGACTCAGCACTTTGATCTTTTTTGAATTCAGTTTCCTTCAAGATCCTCAAGGAAGCTTGGTTGGGGAGCTCTCCACTGGGTGTAAGAGGCTCCAAAACCCCAACCCCAAGCGCAACTCTAAACCTACTTAGTCTCTGCACGATACGGTTAACACAAAGACACCGACAACTTTCTTCAGCACAGCAATACGTAACT
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-@e1dfbb50-890c-4c1c-bd2e-ad2858c054a3 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=11490 ch=369 start_time=2020-03-23T17:37:06Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-GTAGCTACTTCGTTCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTATTGTTAACCGTATCGTGTAGAGACTGCGTAGGTTTAGAGTTGCGCTTGGGGTTGCTTTTGGAGCCCTCTTACACCCAGTGGAGAAGCTCCCCAACCAAGCTCTCTTGAGATCTTGAAACTGAATTCAAAAAGATCAAAGTGCTGAGCTCCGGTGCGTTCGGCACGGTGTATAGTGGGTCCTGGCACGGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGGTGGGGAGCCCAGAGTCCTTGCAAGCTGTATATTTCCATCATCTACTTTACTCTTTGTTTCACTGAGTGTTTGGGAAACTCAGTGTTTTTCCCAAGTTATTGAGGAGGAAATCTTTATAACCACAGTAATCAGAGTGGTCCTGTGAGACCAATTCACAGACCAAAGGCATTTTTATGAAAGGGGCCATTGACCTTGCCATGGGGTGCAGCACAGGGCGGGGAGGAGGGCCGCCTCTCACCACACGGCATCAGAATGCAGCCCAGCTGAAATGGGCTCATCTTCGTTTGCTTCTTCTAGATCCTCTTTGCATGAAATCTGATTTCAGTTAGGCCTAGACGCAGCATCATTAAATTCTGGATGAAATGATCCACACGGACTATAACAGGCTTTACACCAAGCTTGGGGTTCTTTATCCTAAATAATCAGTGTGATTCGTGGAGAGCCCAACAGCTGCAGGGCTGCGGGGCGTCACAGCCCCAACAATATCAGCCTTAGGTGCGGCTCCACCCAGCCCCAGTGTCCCTCACCTTCGGGTGCATCGCTGGTAACATCCACCCAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAATAGGAAATCCTCGATGTGAGTTTTCACGCTTTGCTGTGTGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGAAACTTCAACGCGAACCGTAAGCCCAAACACTCGCACTGACTCGATCACTGCTGGTTAACACAAAAAGACACCGACAACTTTCTTCCAGCACCAGCAATACGTAACT
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-@2d312ed0-34a2-4b89-b6b1-d2c9495f0501 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=10104 ch=59 start_time=2020-03-23T17:38:04Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CGGTAGTATTCGTTCCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTTGTGTTAGCCATGTGATGTAGAGACTGCGTAGGTTTAGAGTTGCGCTTGGGGTTGGGGTTTTGGGCCTCTTGCCCAGTGGAGAAGCTCCCAGCAAGCTCTCTTGAGATCTTGAAGGAAACTGAATTCAAAAAGATCAAAGTGCTGAGCTCCGGTGCGTTCGGCACGGTGTGAGTAGGTCCTGGCACAGGCCTCTGGGCTGGGCCCCTGGGGGCTCTCATGGTCTGGTGGGAGGCCCAGAGTCCTTGCAAGCTGTATATTTCCATCATCTACTTTACTCTTTGTTTCACTGAGTGTTTGGGAAACTCGATGTTTTTCCCAAGTTATTGAGAAATCTTTTATAGCCACAGTAATCAGTGGTCTGTGAGACCAATTCACAGACCAAAGGCATTTTTTATGAGGGCCATTGACCTTGCCATGGGGTGCAGCACAGGGCGGGAGGAGGAGCCGCCTCTCACCGCACGGCATCAGAATGCAGCCCAGCTGAAATGGGCTCATCTTCGTTTTGCTTCTTCTAGATCCTCTTTGCGTGATCTGATTTCAGTTAGGCCTAGACGCAGCATCATTAAATTCTGGATGAAATGATCCACACGGACTTTATAACAGGCTGAAAGCGGGGTTCTTTTATCTAAATAATCAGTGTGATTCGTAGAGCCCAACAGCTGCCAGGGCTGCGGGGCGTCACAGCCCCCAGCAATATCCCAGCCTTAGGTGCGCTCCACAGCCCCAGTGTCCCTCACCTTCGGGGGTGCATCGCGGTAACATCCACCCAGATCACTGGGCAGCATCGTAGCACCATCTCACAATTGCTCAGTTAACGTCTTCCTTCTCTCTCTGTCACTTAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAGGGTAAGAGAAGCAACATCTCCGAAAGCCAACAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGAAACTTCAACGCGAACCGTAGCCCAAACACTCGCACTGACTCGATCACTGGTTAACACAAAGACACCGACAACTTTCTTCAGCACAGCAATGAAC
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-@22540624-2740-45fc-ab6b-55ad4e519dc7 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=6911 ch=220 start_time=2020-03-23T17:38:08Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CTTTTCGTTCGGTTACATCTTGTGGTGCTGAAGAAAATTGTCGGTGTCTTTCGTATTAACCATATCGTGTAGAGACTGCATAGGTTTAGGTTGCCGCTTGGGGTTGGGGTTTTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGATCTTGAAACTGAATTCAAAAGATCAATGCTGGCTCCGGTGCGTTCGGCACGGTGTATAAGGTAAGGTCCCTGGCACAGGCCTCTCTGGAGGCTGGGCCGCAGGGCCTCTCATGGTCTGGTGGGGAGCCCAAGAGTCGCGCCGCAAGCTGTATATTTCCATCATCTACTTTTTACTCTTTGTTTCACTGAGTGTTGGGAAACTCCAGTGTTTTTCCCAGTGTGAGAGGAAATCTTTTATAGCTTACAGTAATCAAGCGTGGTCCTGTGAGACCAATTCACAGACCAAGGCATTTTTATGAAAGGGCCATTGACATATGAGGTGCAGCACAGGGGCGGAGGGGGCCGCCTCTCACCGCGGCATCAGAATTACAGCCCAGCTGAAATGGAAACCATCTTCGTTTGCTTCTTCTAGATCCTCTTTGCATGGAAATCTGATTTCAGTTAGGCCTGAACGCAGCATCATTAAATTCTGGATGAAATGATCCACACGGACTTTATAACAGGCTTTACAAGCTTGGGGTTCTTTTATCTAAATAATCAGTGTGATTCGTGGGCCCAACAGCTGCAGGGCTGCAGGGGGCGTCACAGCCCCAGCAATATCGGCCAGGTGCGGCTCCACAGCCCCAGTGTCCCTCACCTTCGGGGTGCATCTGGTAACATCCCACCAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTATTAACGTCTTCCTTCTCTCTCTGTACTGCCAGGACTCTGGATCCCAGGGTGAAAGTTAAAATTCTTCGTCGCTATCAAGAGATAAGAGAAACATCTCAAAGAAGCCAACAAAGGAAATCCTCAGTATTGCGGTTTCTTTATAATATTGAGGTCCATGGCTCTGAACCTCAGGCCCACCTTTCTCATGTCTGGCAGCTGAAACTTCAACGCGAACCGTAAGCCCAAACACTCGCACTGACTCGATCACTGGTTAACACAAAGACACCGACAACTTTCTTCCAGCACCAGCAATACGTAGC
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-@5bdf58af-d748-4c23-aadf-499e95e621e3 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=9485 ch=35 start_time=2020-03-23T17:38:25Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CAGTAATACTTCGTTCAGTTACGTATTGCTGGTGCTGAAAAAGTTGTCGGTGTCTTTATAACAACAGCATGTGTAGAGACTGCGTAGGTTTAGAGTTGCGCTTGGGGTTGGGGTTTGGAGCCTCTTACACCCAGTGGAAGCTCCCCAACCAAAGCTCTCTTGAGAGATCTTGAAGGAAACTGAATTCAAAAGATCAAAGTGCTGAGCTCCGGTGCGTTCGGCACGGTGTATAAGGTAAGGTCCCTGGCACGGGCCTCTGGGCTGGGCCGCAGACCTCATGGTCTGGTGGGGAGCCCAGAGGTCCTTGCAAGCTGTATATTTCCATCATCTACTTTACTCTTTGTTTCACTGAGTGTTTAGGAAACTCCAGTGTTTTTCCCAAGTTATTGAGAGAAATCTTTTATAACCACAGTAATCAGTGGTCTGTGAGACCAATTCACAGACCAAAGGCATTTATTTTTTATGAAAGGGGCCATTGACCTTGCCATGGGGTGCAGCACAGGGCGGGAGGAGGGCCGCCTCTCTCACACGGCATCAGAATGCAGCCCAGCTGAAATGGGCTCATCTTCGTTTGCTTCTTCTAGATCCTCTTTGCATGAAATCTGATTTCAGTTAAGGCCTAGACGCAGCATCATTAAATTCTGGATGAAATGATCCACACGGACTTTATAACAGGCTTTACAAGCTTGAGGTTCTTTTATCTAAATAATCAGTGTGATTCGTGGAGCCCAACAGCTGCAGGGGCTGCGGGGGCGTCCACAGCCCCCAGCAATATCAGCCCCTTAGGTGCGGCTCCACAGCCCCAGTGTGTCCCTCACCTTCGGGGTGCATCGCTGGTAACATCCACCAGATCACTGGGCAGCATGTAGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGGTGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGTGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCACGAAACTTCAGCATGAACCGTAAGCCCAAACACTCGCACGACTCGATCACTGGTTAGCACAAAGACACCGACAACTTTCTTCTTCAGCACCAGCAATACGTAAC
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-@9f038812-8608-45df-aaae-c0cd7b0365a4 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=11553 ch=177 start_time=2020-03-23T17:38:56Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CAAGTACTTCGTTCAGTTACGTATTGCTGGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAACCAGTGATCGAGTCAGTGCGAGTGTTTGGGCTTACGGTTCGCGTTGAAGTTTCATGCCAGACATGAGAAAAGGTAGAGCCTGAGGTTCAGAGCCATGGACTACACATGTAAGCAGAAACTCACATCAGTTTCCTTGTTGGCTTTCGGAGATGTTGCTTCTCTGTTCCTTGATAGCGACAGAATTTAACTTTCTCACCTTCTGGGATCAAGTCCCTATGACAGAGAGAAGGAAGACGTTAACTGGCAATTGTGAGATGGTGCCACATGCTGCCCAGTGATCTGGGTGGATGTTACCAGCGATGCACCCCGAAGGTGAGGACACTGGGGCTGTGGAGCCGCACTAAGGCTGATATTGCTGGGGGCTGTGACGCCCCCGCAGCCCTGCAGCTGTTGAGGCTCCCACGAATCACACTGATTATTTAGATGAAAATCTCAAGCTTGTAAAGCCTGTTATAAAGTCCGTGTGGATCATTTCATCCAGAATTTGTGATGCTGCGTCTAGGCCTAACTGAAATCAGATTTCATGCAGAGGATCTGAAGAAACAAAAACGAATGAGCCCATTTCGGCTGAACCACATTCTGATGCCGTGCGGTGAGAGGCGGCCCTCCTCCCGCCCTGTGCTGCCCATGGCAAGGTCAATGGCCCCTTTCATAAAAATGCCTTTGGTCTGTGAATTGGTCTCACAGGACCACTGATTACTGTGGTTATAAAGATTTCCTCTAATAGCAGGAAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAAAGTAAAGTAGATGATGGGAAATATACAGCTTGCAGGACTCTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCCAGCCAGAGGCCTGTGCCAGGGACCTTACCTTATACACCGTGCCGAACGCACCGGAGCTCAGCACTTTGATCTTTTTGAATTCAGTTTCCTTCAAGATCTCAAGAGAGCTTGGTTGGGAGCTTCTCCACTGGGTGTAGAGGCTCCAAAACCCCAACCCCAAGCGCAACTCTAAACCTACACCCAGTCTCTACACGATACGGTTAACACAAAGACACAAACAACTTTCTTCAGCACCAGCAATACGTAACT
-+
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
-@53bf9a7b-e983-4968-bbdf-e1c946ea5733 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=10554 ch=426 start_time=2020-03-23T17:39:07Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-TATTCGTTCAGTTACGTATTGCTGGTGCTGAAGAAAGTTGTCCGGTGTCTTTGTGTTAACCGATGATCGAGTCAGTGCAAAGTGTTTGGGCTTACGGTTCGCGTTGAAGTTTCAGCTGCCAGACATGAGAAAAGGTGGGCCTGAGTTCAGAGCCATGGACCCCCACACAGCAAAAGCAGAAACTCACATCGATTTCCTTGTTGGCTTTCGGAGATGTTGCTTCTCTTCATTACTTGATAGCGACAGGAATTTTAACTTTCTCACCTTCTGGGATCCAAGTCCCTATGACAGAGAGAGAAGACGTTAACTGGCAATTGTGAGATGGTGCCACATGCTGCCCAGTGATCTGGGTGGATGTTACCAGCGATTTTGAAGGTGAGGGACACTGGGGCTGTGGAGCCGCACTAAGGCTGATCAGCGCTGGGGGCTGTGACGCCCCCGCAGCCTGCAGCTGTTGGGCTCCACGAATCACACCCCTGATTATTTAGATAAAGAATCTCAAGCTTGTAAAGCCTGTTATAAAGTCCGGTAATGAATCATTTCATCAGAATTTAATGATGCTGCGTCTAGGCCTAGCTTACAAATCAGATTTCATGCAAAGAGGATCTAGAAGCGACGAATGAGCCCATTTCTTGGCTGGGCTTGCATTCTGATGCCGTGCGGTGAGAGGCGGCCCTCCTCCGCCTGTGCTGCACCCCATGGCAAGGTCAATGGCCCCTTTCATAAAAATGCCTTTGGTCTGTGAATTGGTCTCACGGGACCGCTGATTGTGGTTATAAAGATTTCCTCTCAATAACGGAAAAACACGCAGATTTCCCAAACACTCAGTGAAGACAAGTAAAGTAGATGATGGAAATATACAGCTTGCAAGGACTCTGGGCTCCCCACCCAGACCATGAGAGGCCCACGGCCCAGCCCAGGAGCCTGCTTGAGGACCTTACCTTATACACCGTGCCGAACGCACCGGAGCTCAGCACTTTGATCTTTTTGAATTCAGTTTCCTTCAAGATCCTCAAGAGAGCTTGGTTGGGAGCTTCTCCACACAAGGTGTAAGAGGCTCCAAAACCCCAACCCCCAAGCGCAACTCTAAACCTACGCAGTCTCGATACAGTTGCTGCAAAGACACCGACAACTTTCTTCAGCACCAGCAATACGTAAC
-+
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
-@658aa0a8-fa16-47e6-960e-68ffa3d82976 runid=392b802938efed15c9bb1bbc4272f499e4bcd84a read=8405 ch=8 start_time=2020-03-23T17:39:57Z flow_cell_id=FAN54139 protocol_group_id=SS_200323_custom_umi sample_id=917A
-CGGTATTACTTCGTTCCGGTTATGTATTGCTGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCAGTGATCGAGTCAGTGCGAGTGTTTGGGCTTACGGTTCGCGTTGAAGTTTCAGCTGCCAGACATGAGAAAAGGTGGGCCTGAGGTTCAAGAGCCATGGACCCCCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAGATATTTGCTTCTCTTAATTCCTTGATAGCGACAGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTATGACAGAGAGAGAAGGAAGACGTTAACTGGCAATTGTGAGATGGTGCCACATGCTGCCCAGTGATCTGGGTGGATGTTACCAGCGATGCACCCCGAAAGGTGAGGGACACTGGGGCTGTGGAGCACACCTAAGGCTGATATTGCTGGGGGCTGTGACGCCCCACGCAGCCTGCAGCTGTTGGGCTCCACGAATCACACTGATTATTTAGATAAAAGAATCTCAAGCTTGTAAAGAAGCCTGTTATAAAGTCCATTGTGGATCATTTCATCCAGAATTTAATGATGCTGCGTCTAGGCCTAACTGAAATCAGATTTCATGCAAGAGGATCTAGAAGAAGCAAACGAAGATGAGCCCATTTCAGCTGGGCTGCATTCTGATGCCGTGCGGTGACGGCCCTCCTCCCGCCCTGTGCTGCACCCCATGGCAAGGTCAATGGCCCCTTTCATAAAAATGCCTTTGGTCTGTGAATTGGTCTCACAGGACCACTGATTACTGTGGTTATAAAGATTTCCTCTCAATAACTTGGGAAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAGTAAAGTAGATGATGGAAATATACAGCTTCCAAGGACTCTGGGCTCCCACCAGACCATGAGAGGCCCTGCGGCCCAGCCCAGAGGCCTGTGCCAGGGACCTTACCTTATACACCGTGCCGAACGCACCCGGAGCTCCAGCACTTTGATCTTTTTTGAATTCAGTTTCCTTCCAAGATCCTCAAGAGAGCTTGGTTGGGAGCTTCTCCACTGGGTGTAAGAGGCTCCAAACCCCAACCCCAAGCGCAACTCTAAACCTACGCAGTCTCTACGATACGGTTAACACAAAAGACACCGACAACTTTCTTCGCTTTGACAATACGTAACT
-+
-'%,**)+,2279<46BCB@D=7-=4?AEC=6>6@CA;=EDD0@A6;A>93+1>2/258/5*3).*#(,*1;8F@E'11+23CG3;ED3CFDH9B39KBK5@:7@B3BEDBKF;BED>:>1)6<4?==;<=967=<6;'(,2&79/6:;=@@E1-4/A?B::A@?)6++,38;>A88.';<;=D>;;8'(8?;9=8;>KE-947:36.,@@A'7>BAHCDA?@:<8=C9->;A4781+'2,-/0467;:6:>>>=BE>@?-00.4:>++8C/1A:8;C@;9>?-5:1,/:>?:$:+=:;2>68:>A@)'%%%''';AAEE==>:=>)1114''),534;7;;?BI@5((<66/28;=<..@66945;>BA17-/(,8GA,DMHA>?>DCJ>=E@ADHJ9=555:>,435=><=DED@A=A@;?C?@=DE?F<9:8FBA23*+&5+3@A8:*0.=BEBCD@A=>:785%(C>@:.&'48348A8A;?E>9**8;8GG=A=DC@9>332974-311-./=@0620//78.*&*55907C@DG9/;?BE<=>:8E>?766((&*/07;20-(&&1264@FHI?DA--7:4=::HDDA@24>@C@<67>?;77&*:%'AB3=?A4528'+=B>66=;?::::@A;:=>5?8>2/;142//-0;9;<=;<72(4/6542/445:7;=@@DD=93E;:+**-,()2087*/',99%?>6?B33:4?=99EFKB>/*,,25676;;B+/+>)79:>C668@CCD83,:6ABA=AKB/22/0;=>=@9@;?E=<:920''2-%.**.2'*-355499A?>?7::C3B<=C7@-9=554()+17670=?B5(9=;@BG:AKGCB?:?DF1(8B=E>;9I?==AEE?>>5.$$$'*)*3;<<H42.1:@:>D@@93432..=9CDF?;A;>BE92&%:%-&'->8BC>9:<,,2=HCEJGFBC@@<>=?32A;<99BDCA<36/$1474'4?0&49/3<:=>+-J:.:-68DC=C:=9,-+*%$(''(-.()1&'%%'.(%&3362(0(5I;<:>?@8,EE==C=B:@>>CF?D058@HB:D46::8%+@DG@>??79<;>;))-;+&BHE=A@62%231--=:2;<6.(24?A=-$<<;<;=6498@CBBDCC=C7@7''/8,6626B>::;6,-6=BD??632-67&'3..5*&'.23311,)17ACDGB?;=