diff --git a/docs/articles/Omix_vignette_MAP_CV_pseudotemporal.html b/docs/articles/Omix_vignette_MAP_CV_pseudotemporal.html index 1def2fc..87996a5 100644 --- a/docs/articles/Omix_vignette_MAP_CV_pseudotemporal.html +++ b/docs/articles/Omix_vignette_MAP_CV_pseudotemporal.html @@ -88,7 +88,7 @@

Omix - Pseudotemporal multi-omics

This vignette was created by Eléonore Schneegans and Nurun Fancy

-

20 July, 2023

+

12 January, 2024

@@ -171,13 +171,13 @@

Running OmixGO_fp <- file.path(outputDir, "GO_Biological_Process_2021.txt") -download.file(url = "https://raw.githubusercontent.com/eleonore-schneeg/OmixData/main/ctd-2.rds",destfile=ctd_fp ,headers = c(Authorization = paste("token", Sys.getenv("GH_TOKEN")))) +download.file(url = "https://raw.githubusercontent.com/eleonore-schneeg/OmixData/main/ctd-2.rds",destfile=ctd_fp) -download.file(url = "https://raw.githubusercontent.com/eleonore-schneeg/OmixData/main/ensembl_mappings_human.tsv",destfile=ensembl_fp ,headers = c(Authorization = paste("token", Sys.getenv("GH_TOKEN")))) +download.file(url = "https://raw.githubusercontent.com/eleonore-schneeg/OmixData/main/ensembl_mappings_human.tsv",destfile=ensembl_fp) -download.file(url = "https://raw.githubusercontent.com/eleonore-schneeg/OmixData/main/Enrichr_Queries.gmt.txt",destfile=TF_fp ,headers = c(Authorization = paste("token", Sys.getenv("GH_TOKEN")))) +download.file(url = "https://raw.githubusercontent.com/eleonore-schneeg/OmixData/main/Enrichr_Queries.gmt.txt",destfile=TF_fp) -download.file(url = "https://raw.githubusercontent.com/eleonore-schneeg/OmixData/main/GO_Biological_Process_2021.txt",destfile=GO_fp ,headers = c(Authorization = paste("token", Sys.getenv("GH_TOKEN")))) +download.file(url = "https://raw.githubusercontent.com/eleonore-schneeg/OmixData/main/GO_Biological_Process_2021.txt",destfile=GO_fp) synapser::synLogin(email=Sys.getenv("SYNAPSE_ID"), password=Sys.getenv("SYNAPSE_PASSWORD")) @@ -677,7 +677,7 @@

Ext
 weights=extract_weigths(integrated_object,
                            factor=6,
-                           threshold=0.2,
+                           threshold=1.5,
                            sense_check_variable='PHF1')

Weights distribution in Factor 6 @@ -721,20 +721,17 @@

Selected features based on
  • rlength(weights\(weights\)ranked_weights_negative$rna)` RNAs have weights below -0.3 in factor 6

  • -
  • 327 proteins have weights below -0.3 in factor 6

  • +
  • 176 proteins have weights below -0.3 in factor 6

Some driving features may overlap between transcriptomics and proteomics

 intersect(weights$weights$ranked_weights_negative$rna,weights$weights$ranked_weights_negative$protein)
-
##  [1] "ANXA1"   "B2M"     "ICAM5"   "COL18A1" "S100A10" "C4A"     "GFAP"   
-##  [8] "RLBP1"   "ITPR1"   "S100A4"  "GJA1"    "VIM"     "PRELP"   "CLIC1"  
-## [15] "AKAP5"   "FLNA"    "HSPG2"   "CAPS"    "AHNAK"   "COL6A2"  "CAPG"   
-## [22] "TAGLN"   "FABP7"   "C1QA"    "MYL9"    "F13A1"   "C3"      "COL14A1"
-## [29] "OGN"     "ANXA2"   "C1QC"    "CRABP1"  "DCN"     "COL1A1"  "COL6A3" 
-## [36] "CALB2"
+
##  [1] "AKAP5"   "FLNA"    "HSPG2"   "AHNAK"   "COL6A2"  "CAPG"    "TAGLN"  
+##  [8] "FABP7"   "F13A1"   "C3"      "COL14A1" "ANXA2"   "C1QC"    "CRABP1" 
+## [15] "DCN"     "COL1A1"  "CALB2"
    -
  • 13 features overlap between omic layers
  • +
  • 7 features overlap between omic layers

@@ -785,7 +782,7 @@

Community detection withi list(extend = 'csv', exportOptions = list(columns=':visible')), list(extend = 'excel', exportOptions = list(columns=':visible')))))
-