diff --git a/manual-est/images.log b/manual-est/images.log
index 988e656..0176e44 100644
--- a/manual-est/images.log
+++ b/manual-est/images.log
@@ -1,4 +1,4 @@
-This is pdfTeX, Version 3.14159265-2.6-1.40.20 (TeX Live 2019/Debian) (preloaded format=pdflatex 2024.3.15)  13 JUN 2024 09:14
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diff --git a/manual-est/images.pdf b/manual-est/images.pdf
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diff --git a/manual-est/images.tex b/manual-est/images.tex
index 071b556..c7506c5 100644
--- a/manual-est/images.tex
+++ b/manual-est/images.tex
@@ -118,20 +118,20 @@
   \else\expandafter\vss\fi}%
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-\providecommand{\Zeta}{\textrm{Z}}
-\providecommand{\Nu}{\textrm{N}}
-\providecommand{\Omicron}{\textrm{O}}
-\providecommand{\Chi}{\textrm{X}}
+\providecommand{\Beta}{\textrm{B}}
+\providecommand{\Alpha}{\textrm{A}}
 \providecommand{\Rho}{\textrm{R}}
-\providecommand{\Iota}{\textrm{J}}
 \providecommand{\Mu}{\textrm{M}}
-\providecommand{\omicron}{\textrm{o}}
-\providecommand{\Alpha}{\textrm{A}}
+\providecommand{\Zeta}{\textrm{Z}}
+\providecommand{\Chi}{\textrm{X}}
 \providecommand{\Epsilon}{\textrm{E}}
-\providecommand{\Beta}{\textrm{B}}
+\providecommand{\Omicron}{\textrm{O}}
+\providecommand{\Kappa}{\textrm{K}}
+\providecommand{\Nu}{\textrm{N}}
+\providecommand{\Iota}{\textrm{J}}
 \providecommand{\Tau}{\textrm{T}}
+\providecommand{\omicron}{\textrm{o}}
+\providecommand{\Eta}{\textrm{H}}
 
 
 \begin{document}
diff --git a/manual-est/index.html b/manual-est/index.html
index 2ba3ef9..cbd92ee 100644
--- a/manual-est/index.html
+++ b/manual-est/index.html
@@ -351,7 +351,7 @@ <H2><A ID="SECTION00032000000000000000"></A> <A ID="binaries"></A>
 can be easily downloaded from the <A ID="tex2html19"
   HREF="ftp://ftp.ncbi.nlm.nih.gov/blast/executables/">NCBI FTP</A>
 site.
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+The packed binaries are <SPAN  CLASS="textit">blastp</SPAN> and <SPAN  CLASS="textit">makeblastdb</SPAN> from version <SPAN  CLASS="textit">ncbi-blast-2.16.0+</SPAN>. If these do
 not work in your machine or your prefer to use older BLAST versions, then it will be necessary to
 edit file <SPAN  CLASS="textit">lib/phyTools.pm</SPAN>. First, environmental variable <code>$ENV{'BLAST_PATH'}</code> needs to be set to 
 the right path in your system (inside subroutine <code>sub set_phyTools_env</code>).
@@ -2821,7 +2821,7 @@ <H1><A ID="SECTION00090000000000000000">
 The command line arguments were: <BR>
  <kbd>latex2html manual-est -no_antialias_text -split 0 -dir manual-est -no_navigation -show_section_numbers</kbd>
 <P>
-The translation was initiated on 2024-06-13
+The translation was initiated on 2025-01-08
 <BR><HR>
 
 </BODY>
diff --git a/manual-est/manual-est.html b/manual-est/manual-est.html
index 2ba3ef9..cbd92ee 100644
--- a/manual-est/manual-est.html
+++ b/manual-est/manual-est.html
@@ -351,7 +351,7 @@ <H2><A ID="SECTION00032000000000000000"></A> <A ID="binaries"></A>
 can be easily downloaded from the <A ID="tex2html19"
   HREF="ftp://ftp.ncbi.nlm.nih.gov/blast/executables/">NCBI FTP</A>
 site.
-The packed binaries are <SPAN  CLASS="textit">blastp</SPAN> and <SPAN  CLASS="textit">makeblastdb</SPAN> from version <SPAN  CLASS="textit">ncbi-blast-2.14.0+</SPAN>. If these do
+The packed binaries are <SPAN  CLASS="textit">blastp</SPAN> and <SPAN  CLASS="textit">makeblastdb</SPAN> from version <SPAN  CLASS="textit">ncbi-blast-2.16.0+</SPAN>. If these do
 not work in your machine or your prefer to use older BLAST versions, then it will be necessary to
 edit file <SPAN  CLASS="textit">lib/phyTools.pm</SPAN>. First, environmental variable <code>$ENV{'BLAST_PATH'}</code> needs to be set to 
 the right path in your system (inside subroutine <code>sub set_phyTools_env</code>).
@@ -2821,7 +2821,7 @@ <H1><A ID="SECTION00090000000000000000">
 The command line arguments were: <BR>
  <kbd>latex2html manual-est -no_antialias_text -split 0 -dir manual-est -no_navigation -show_section_numbers</kbd>
 <P>
-The translation was initiated on 2024-06-13
+The translation was initiated on 2025-01-08
 <BR><HR>
 
 </BODY>
diff --git a/manual/images.log b/manual/images.log
index a9d3dd7..0e32b89 100644
--- a/manual/images.log
+++ b/manual/images.log
@@ -1,4 +1,4 @@
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 entering extended mode
  restricted \write18 enabled.
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diff --git a/manual/images.pdf b/manual/images.pdf
index 7cb2b5a..bbdb272 100644
Binary files a/manual/images.pdf and b/manual/images.pdf differ
diff --git a/manual/images.tex b/manual/images.tex
index e388ab3..5c2806a 100644
--- a/manual/images.tex
+++ b/manual/images.tex
@@ -118,20 +118,20 @@
   \else\expandafter\vss\fi}%
 \providecommand{\selectlanguage}[1]{}%
 \makeatletter \tracingstats = 1 
-\providecommand{\Eta}{\textrm{H}}
+\providecommand{\Chi}{\textrm{X}}
+\providecommand{\Rho}{\textrm{R}}
+\providecommand{\Omicron}{\textrm{O}}
 \providecommand{\Tau}{\textrm{T}}
-\providecommand{\Beta}{\textrm{B}}
 \providecommand{\Alpha}{\textrm{A}}
-\providecommand{\Zeta}{\textrm{Z}}
 \providecommand{\omicron}{\textrm{o}}
-\providecommand{\Iota}{\textrm{J}}
-\providecommand{\Omicron}{\textrm{O}}
-\providecommand{\Rho}{\textrm{R}}
-\providecommand{\Mu}{\textrm{M}}
+\providecommand{\Eta}{\textrm{H}}
 \providecommand{\Nu}{\textrm{N}}
-\providecommand{\Chi}{\textrm{X}}
 \providecommand{\Kappa}{\textrm{K}}
 \providecommand{\Epsilon}{\textrm{E}}
+\providecommand{\Mu}{\textrm{M}}
+\providecommand{\Iota}{\textrm{J}}
+\providecommand{\Beta}{\textrm{B}}
+\providecommand{\Zeta}{\textrm{Z}}
 
 
 \begin{document}
diff --git a/manual/index.html b/manual/index.html
index b89a8e2..526f427 100644
--- a/manual/index.html
+++ b/manual/index.html
@@ -348,7 +348,7 @@ <H2><A ID="SECTION00032000000000000000"></A> <A ID="binaries"></A>
 can be easily downloaded from the <A ID="tex2html20"
   HREF="ftp://ftp.ncbi.nlm.nih.gov/blast/executables/">NCBI FTP</A>
 site.
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+The packed binaries are <SPAN  CLASS="textit">blastp</SPAN> and <SPAN  CLASS="textit">makeblastdb</SPAN> from version <SPAN  CLASS="textit">ncbi-blast-2.16.0+</SPAN>. If these do
 not work in your machine or your prefer to use older BLAST versions, then it will be necessary to
 edit file <SPAN  CLASS="textit">lib/phyTools.pm</SPAN>. First, environmental variable <code>$ENV{'BLAST_PATH'}</code> needs to be set to 
 the right path in your system (inside subroutine <code>sub set_phyTools_env</code>).
@@ -4171,7 +4171,7 @@ <H1><A ID="SECTION00090000000000000000">
 The command line arguments were: <BR>
  <kbd>latex2html manual -no_antialias_text -split 0 -dir manual -no_navigation -show_section_numbers</kbd>
 <P>
-The translation was initiated on 2024-06-13
+The translation was initiated on 2025-01-08
 <BR><HR>
 
 </BODY>
diff --git a/manual/manual.html b/manual/manual.html
index b89a8e2..526f427 100644
--- a/manual/manual.html
+++ b/manual/manual.html
@@ -348,7 +348,7 @@ <H2><A ID="SECTION00032000000000000000"></A> <A ID="binaries"></A>
 can be easily downloaded from the <A ID="tex2html20"
   HREF="ftp://ftp.ncbi.nlm.nih.gov/blast/executables/">NCBI FTP</A>
 site.
-The packed binaries are <SPAN  CLASS="textit">blastp</SPAN> and <SPAN  CLASS="textit">makeblastdb</SPAN> from version <SPAN  CLASS="textit">ncbi-blast-2.14.0+</SPAN>. If these do
+The packed binaries are <SPAN  CLASS="textit">blastp</SPAN> and <SPAN  CLASS="textit">makeblastdb</SPAN> from version <SPAN  CLASS="textit">ncbi-blast-2.16.0+</SPAN>. If these do
 not work in your machine or your prefer to use older BLAST versions, then it will be necessary to
 edit file <SPAN  CLASS="textit">lib/phyTools.pm</SPAN>. First, environmental variable <code>$ENV{'BLAST_PATH'}</code> needs to be set to 
 the right path in your system (inside subroutine <code>sub set_phyTools_env</code>).
@@ -4171,7 +4171,7 @@ <H1><A ID="SECTION00090000000000000000">
 The command line arguments were: <BR>
  <kbd>latex2html manual -no_antialias_text -split 0 -dir manual -no_navigation -show_section_numbers</kbd>
 <P>
-The translation was initiated on 2024-06-13
+The translation was initiated on 2025-01-08
 <BR><HR>
 
 </BODY>