diff --git a/README.MD b/README.MD
index 3548a2a..987f3e1 100644
--- a/README.MD
+++ b/README.MD
@@ -43,6 +43,7 @@ You need only construct the environment from `conda env create --prefix=./spechl
 - SpecHLA does not accept short single-end reads.
 - The conda environment should be located in the local folder.
 - In case of failed installation, pls check your GCC version, it should be `GCC 9.4.0+`, see https://github.com/deepomicslab/SpecHLA/issues/42
+- Please ensure to clear the previous results before rerunning SpecHLA.
 
 
 ## Test