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metadata.yaml
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about: []
access:
- schemaKey: AccessRequirements
status: dandi:OpenAccess
assetsSummary:
approach: []
dataStandard:
- identifier: RRID:SCR_015242
name: Neurodata Without Borders (NWB)
schemaKey: StandardsType
measurementTechnique: []
numberOfBytes: 2762531957
numberOfFiles: 5
numberOfSubjects: 5
schemaKey: AssetsSummary
species:
- identifier: http://purl.obolibrary.org/obo/NCBITaxon_9606
name: Homo sapiens - Human
schemaKey: SpeciesType
variableMeasured: []
citation: Silkunas, Mantas; Silkuniene, Giedre; Pakhomov, Andrei (2024) Visualization
and characterization of membrane lesions (Version 0.240511.1846) [Data set]. DANDI
archive. https://doi.org/10.48324/dandi.000938/0.240511.1846
contributor:
- identifier: 0000-0002-4568-9265
includeInCitation: true
name: Silkunas, Mantas
schemaKey: Person
- affiliation: []
email: giedre.silkuniene@gmail.com
includeInCitation: true
name: Silkuniene, Giedre
roleName:
- dcite:ContactPerson
schemaKey: Person
- identifier: 0000-0003-3816-3860
includeInCitation: true
name: Pakhomov, Andrei
schemaKey: Person
- awardNumber: NIH 15R21EY034803
includeInCitation: false
name: National Institutes of Health
roleName:
- dcite:Funder
schemaKey: Organization
- awardNumber: NIH 1R21EY034258
includeInCitation: false
name: National Institutes of Health
roleName:
- dcite:Funder
schemaKey: Organization
dateCreated: '2024-03-12T19:12:10.562597+00:00'
datePublished: '2024-05-11T18:46:23.398745+00:00'
description: In this study, real-time imaging was used to visualize individual electropores
and demonstrate their persistence in electroporated cells. HEK 923 cells were cultured
on Indium Tin Oxide (ITO)-coated coverslips and loaded with the Cal520 fluorescent
indicator for visualization. Real-time imaging was performed in Total Internal Reflection
Fluorescent (TIRF) mode. Electrophysiological manipulations were applied using a
patch clamp in voltage clamp mode, set in a whole-cell configuration. The protocol
included a command voltage of 0 mV interrupted by a -50 mV pulse for 50 ms and a
-400 mV pulse for 1 ms at frame 91, returning to -50 mV for 50 ms. Additionally,
0.4 seconds into the second and third stacks, the command voltage was changed from
0 mV to -50 mV for 50 ms. The study was supported in part by NIH 15R21EY034803 and
NIH 1R21EY034258.
doi: 10.48324/dandi.000938/0.240511.1846
ethicsApproval: []
id: DANDI:000938/0.240511.1846
identifier: DANDI:000938
keywords: []
license:
- spdx:CC-BY-4.0
manifestLocation:
- https://dandiarchive.s3.amazonaws.com/dandisets/000938/0.240511.1846/assets.yaml
name: Visualization and characterization of membrane lesions
protocol: []
publishedBy:
endDate: '2024-05-11T18:46:23.398745+00:00'
id: urn:uuid:7145e20f-26dd-4118-9b9b-80a9edb6e8f9
name: DANDI publish
schemaKey: PublishActivity
startDate: '2024-05-11T18:46:23.398745+00:00'
wasAssociatedWith:
- id: urn:uuid:1bc432a4-c7c7-44a3-8e9e-a6652b7b0369
identifier: RRID:SCR_017571
name: DANDI API
schemaKey: Software
version: 0.1.0
relatedResource: []
repository: https://dandiarchive.org
schemaKey: Dandiset
schemaVersion: 0.6.6
studyTarget: []
url: https://dandiarchive.org/dandiset/000938/0.240511.1846
version: 0.240511.1846
wasGeneratedBy:
- identifier: 1R21EY034258
name: Targeted Neuromodulation by Nanosecond Pulsed Electric Fields
schemaKey: Project
- identifier: R21EY034803
name: Next Generation Temporal Interference Stimulation for Non-Invasive Neuromodulation
schemaKey: Project