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2020 Pittsburgh CCDL Training @ Carnegie Mellon University

Traning material developed by Childhood Cancer Data Lab
Alex's Lemonade Stand Foundation https://www.alexslemonade.org

Location: Mellon Institute Library, 4th Floor Mellon Institute

Dates: 03/24/2020 - 03/26/2020

To apply: https://forms.gle/a4uHbSRDHrsx13dP7

Tentative Schedule

Time Topic
Day 0 (03/24) Pre-workshop to set up your computer
3 -5:00 PM Getting Started: Install Docker, Get files from Box, Docker container set up
Day 1 (03/25)
9:00 Welcome
9:15 Introduction to R
10:00 R Intermediate Topics (including Tidyverse)
10:45 Exercises Introduction: Intro to R, Intro to Tidyverse
11:30 PM Bulk RNA-seq I: QC, trim, and quantification with Salmon
12:00 Lunch
1:00 Bulk RNA-seq II: Gene-Level Summaries with tximport
1:45 Bulk RNA-seq III: Exploratory Analyses
2:00 Bulk RNA-seq IV: Differential Expression Analysis: tximport, Differential Expression
3:30 Exercise Introduction: Bulk RNA-seq
3:45 Break
4:00 Machine Learning: Data Preparation, Clustering, PLIER, Differential PLIER LVs
5:15 Exercise Introduction: Machine Learning
5:30 End
6:00 Dinner: TBD
Day 2 (03/26)
9:00 AM Analyze your own data (or some stuff we have)!
12:30 PM Lunch
2:00 Presentations, interpretations, and discussion
3:30 Break
4:00 Talk from CCDL data science team and wrap-up
5:00 Adjourn