Process dual-sgRNA CRISPR functional genetic screening data
Install nextflow (DSL2) following the instructions at https://www.nextflow.io/docs/latest/getstarted.html
Install apptainer following the instructions at
https://apptainer.org/docs/user/latest/quick_start.html#installation
The most convenient way is to install dual-crispr-process-nf is to use nextflow's built-in pull command
nextflow pull zuberlab/dual-crispr-process-nfBefore you start, make sure nextflow and apptainer are properly installed on your system.
Clone git repository from Github and run the pipeline using the provided test data.
git clone https://github.com/ZuberLab/dual-crispr-process-nf.git
cd dual-crispr-process-nf
./testnextflow run zuberlab/dual-crispr-process-nf --help