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Are Casanovo per-AA scores reversed? #408

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justin-a-sanders opened this issue Dec 3, 2024 · 2 comments · Fixed by #417
Closed

Are Casanovo per-AA scores reversed? #408

justin-a-sanders opened this issue Dec 3, 2024 · 2 comments · Fixed by #417
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bug Something isn't working

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@justin-a-sanders
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Describe the Issue

It looks like the list of per-amino-acid scores reported by Casanovo might be reversed. For most incorrect predictions, it looks like the lowest scoring amino acids would line up better with the sequencing errors if the list of scores was reversed. For example:

Casanovo peptide: TSSGGTAALGCLVK	
Tide peptide:     STSGGTAALGCLVK	
Per-AA scores: 0.98486,0.98473,0.98619,0.98608,0.98527,0.98520,0.98420,0.98520,0.98551,0.98500,0.98550,0.98627,0.91044,0.98314

Peptides are sequenced from right-to-left and then reversed to get the final prediction. Are the corresponding per-aa scores also reversed accordingly?

@justin-a-sanders justin-a-sanders added the bug Something isn't working label Dec 3, 2024
@bittremieux
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Can you please confirm the Casanovo version you're using?

@justin-a-sanders
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I was seeing this in results from Casanovo version 4.2.1

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