cell_setup_pc2b_control.hoc

/* ____________      CELL SET-UP PROCEEDURE      _____________ */

RmSoma=149999//
RaSoma=42.562//
RmTuft=45373.4//
RaTuft=35//
DistHalfRm=151.741
DistHalfRa=90.8296
SlopeRm=13.8656
SlopeRa=7.76766
soma_hbar = 0.00003//
KirGbar          = 0.00020307*5//5   // Maximum conductance of potassium inward rectifier.
Epas=-71.9879
CmSoma=1


SpineFactorBasal=3.5
SpineFactorTuft=3.5

//curbase was 0.05 in the soma and 0.06 in the dendrites
balance = 0
geomnseg = 1

strdef sectype
objref CAN_temp, CAL_temp, CAT_temp, KAD_temp, KAP_temp, NA_temp


//RTH>> set distance ref
soma distance()

// SEVERELY affects experiment results
celsius      = 34 // Temperature of slice.")
        
//NDB >>>>  $o1.xopen_library("Terrence","cut-sections")
//          cut_sections(maximum_segment_length)
//maximum_segment_length=75
//forall {
  //  nseg=1+int(L/maximum_segment_length)
//}
//<<<< NDB

//ORIG>>// make 3-d mapping of cell sections
//NDB >>>>    $o1.xopen_library("Terrence","map-segments-to-3d")
//           map_segments_to_3d()
forall {
	insert d3
	i=0
	x_d3(0)=x3d(0)
	y_d3(0)=y3d(0)
	z_d3(0)=z3d(0)

	for (x) if (x > 0 && x < 1) {
		while (arc3d(i)/L < x) { i += 1 }
		D=arc3d(i) - arc3d(i-1)
		if (D <= 0) {
			printf("\t\t * %s had a D < 0\n", secname())
		}
		alpha = (x*L - arc3d(i-1))/D
		x_d3(x)=x3d(i-1) + (x3d(i) - x3d(i-1))*alpha
		y_d3(x)=y3d(i-1) + (y3d(i) - y3d(i-1))*alpha
		z_d3(x)=z3d(i-1) + (z3d(i) - z3d(i-1))*alpha
	}

	x_d3(1)=x3d(n3d()-1)
	y_d3(1)=y3d(n3d()-1)
	z_d3(1)=z3d(n3d()-1)
}
//NDB <<<<

//ORIG>>// prepare to make a graph with cell configuration
//NDB >>>>    $o1.tmpo2=new Shape()
objref tmpo2
tmpo2=new Shape()
//NDB <<<<  

//ORIG>>// Set initial conductance values 
mult_cal=1
soma_caL =0.00006*mult_cal
soma_car =0.00003*mult_cal
gsomacar =0.00008*mult_cal
soma_caLH =0// 0.00018
soma_caT =0.0003*mult_cal
soma_km=0*0.001
potNa=50
mykca_init =0*0.9*1.5*0.03 //0.03 flag
soma_kca =0*0.7*4.5*0.0001//0.003 flag
AXKdr=1
AXNa=3.5//.5//.25

gkdrdend=0
gnanotrunk=0

psoma=0.00075//0.0015//0.01
slowsoma=0.15
slownotsoma=0.1
sinfsoma=1.35//1.35

soma_kap =7*0.0005*4*2.75//4//
axon_kap =7*0.0005*4*4//4//
soma_kad =7*0.0005*4*2.75//4//

gna=0.035*1//
axongkdr=0.011//
//gkdrapical=0.01*0.05//
gkd=0.005


gnadend=0.015*1.5//1.5
gkdrapical=0.01*0.05//



gkv2soma=0.00264*5//*0.05//
gkv2=0.00198*10//*0.05//
gkv2axon=0.00198*10//*0.05//
gkv2scale=0.3
scale_Na_conduct=14//15//13for C1O1 v2 0 everywhere 16 for step function
gkdrsoma=gkdrapical*0

distalv=0
proximalv=6



icanfrac= 0.0
icangbar = 0.06*1//*2.75//22//0.065//0.05//*3
cantau = 400//80

gip3= 1.85 // varies alpha as conductance of IP3R

// ***************************************************
// * Definition of exponential function that doesn't *
// * produce out-of-range values.                    *
// ***************************************************

func MyExp() {
    if ($1>50) {
        return exp(50)
    } else {
        if ($1<-50) {
            return 0
        } else {
            return exp($1)
        }
    }
}

//ORIG>>// Start inserting mechanisms in cell
sectype ="soma"
forsec "soma" {

	insert ican
		gbar_ican = icangbar
		taur_ican = cantau
	
	insert na16a
	
		gbar_na16a= gna * scale_Na_conduct 
		
		dist_na16a = sinfsoma
		
		persist_na16a = psoma
		
		slowdown_na16a = slowsoma
		
		C1O1v2_na16a=proximalv
		
	insert kd
		gbar_kd=gkdrsoma
		//shift_kdr=shiftkdr
		//scale_kdr=scalekdr
	ena         = potNa
	
	insert Kv2like
	gbar_Kv2like=gkv2soma
	insert nap  //flag
		gnabar_nap = 0*.5*0.000014 
		K_nap = 4.5
		vhalf_nap = -60.4
	
	insert pas
    Rm=RmTuft+1*(RmSoma-RmTuft)/(1+MyExp((-DistHalfRm)/SlopeRm))
    Ra=RaTuft+1*(RaSoma-RaTuft)/(1+MyExp((-DistHalfRa)/SlopeRa))
    g_pas=1/Rm
    cm=CmSoma
	
	insert h     // h current 
		gbar_h  = soma_hbar
		K_h     = 8.8
		vhalf_h = -82
	insert kap  // proximal A current
		gkabar_kap = soma_kap
	ek         = -80
	insert km  // m-type potassium current
		gbar_km    = soma_km
	ek         = -80 
	insert cal // HVA Ca++-L type current
		gcalbar_cal = 0.1*soma_caL
	insert cat // LVA Ca++-T type current
		gcatbar_cat = soma_caT
        //insert somacar // HVAm Ca++-R type current
    	//gcabar_somacar = gsomacar
	insert car // HVAm Ca++-R type current
	gcabar_car = gsomacar
	insert kca   // K(Ca) mAHP potassium type current
		cac_kca=0.00075 //0.0005
		gbar_kca = 0.5*soma_kca
	insert mykca // K(Ca) fAHP potassium type current
		gkbar_mykca = 5.5*mykca_init        
	//insert cad  // calcium pump/buffering mechanism 
		//taur_cad=20
	//insert cabalan  // calcium pump/buffering mechanism 
	tmpo2.color(2) 

}

//ORIG>>  Configure Axon

sectype="axon"
forsec axon_sec_list {	  
	insert nax
		gbar_nax=gna*AXNa
	insert kd
		gbar_kd=axongkdr
		//shift_kdr=shiftkdr
		//scale_kdr=scalekdr
	ena         = potNa
	
	insert pas
    Rm=RmTuft+1*(RmSoma-RmTuft)/(1+MyExp((-DistHalfRm)/SlopeRm))
    Ra=RaTuft+1*(RaSoma-RaTuft)/(1+MyExp((-DistHalfRa)/SlopeRa))
    g_pas=1/Rm
    cm=CmSoma
    
	insert km  // m-type potassium current
		gbar_km     = 3*soma_km
		ek          = -80
	insert kap  // proximal A current
	   gkabar_kap = axon_kap
	   ek         = -80
	insert Kv2like
		gbar_Kv2like=gkv2axon
}
    
//ORIG>>  Configure apical trunk
 
forsec apical_trunk_list {

	insert ican
		gbar_ican = icangbar
		taur_ican = cantau

	//ORIG>>// apical_h_insert_sig($o1)    // Inserting h-current
	//NDF>>>	apical_caR_caLH_insert($o1) // Inserting Ca++ R-type and Ca++ L-type currents
	for (x) {  
		xdist = find_vector_distance_precise(secname(),x)
		xdist=distance(x)
		insert car
		gcabar_car(x) = 0.1*soma_car
		insert calH
		if (xdist > 50) {            
			gcalbar_calH(x) = 2*soma_caLH    //4.6*soma_caLH
		} else {
			gcalbar_calH(x) = 0.1*soma_caLH               //0.1*soma_caLH
		}
	}
	//<<<NDF
	//NDF>>> apical_caT_insert($o1)      // Inserting Ca++ T-type current
	caT_distal_maxfactor = 4   //ORIG>> maximum cond. factor in dendrites
	caT_distal_distance  = 350 //ORIG>> distance in dendrites for maximum cond.
	for (x) {  
		xdist = find_vector_distance_precise(secname(),x)
		xdist = distance(x)
		fr = xdist/caT_distal_distance
		insert cat  
		if (xdist < 100) {
			gcatbar_cat(x) = 0
		} else {
			gcatbar_cat(x) = caT_distal_maxfactor*soma_caT*fr
		} 
	}
	//<<<NDF
	//NDF>>> apical_kca_insert($o1)      // Inserting K(Ca) sAHP and mAHP potassium currents
	kca_distal_maxfactor = 1   //ORIG>> maximum cond. factor in dendrites
	//ORIG>>// kca_distal_maxfactor = 0 //maximum cond. factor in dendrites
    kca_distal_distance  = 200 //ORIG>> distance in dendrites for maximum cond.
	for (x) {  
		xdist = find_vector_distance_precise(secname(),x)
		xdist = distance(x)
		fr = xdist/kca_distal_distance 
		//insert cad    // calsium pump/buffering mechanism
			//taur_cad=20
		insert kca    // slow AHP K++ current
			cac_kca=0.00075 //0.0005
		insert mykca  // medium AHP K++ current

		if (xdist < kca_distal_distance && xdist > 50) {       
			gbar_kca(x) = 5*soma_kca
			gkbar_mykca = 2*mykca_init
		} else {
			gbar_kca(x) = 0.5*soma_kca
			gkbar_mykca = 0.5*mykca_init
		}
	}
	//<<<NDF
	//ORIG>>// A_insert($o1)               // Inserting A-current
	insert h
	insert kap
	insert kad
	insert Kv2like
	

	for (x){ 
		xdist = distance(x)
		if (xdist>500) {xdist=500}
		gbar_h(x) = soma_hbar*(1+(6/5)*xdist/100)
		if (xdist > 100){
			if (xdist>300) {ndist=300} else {ndist=xdist}
			vhalf_h(x)=-81-8*(ndist-100)/200
			gkabar_kad(x) = soma_kad*(1+xdist/100)
			gkabar_kap(x)=0
			gbar_Kv2like(x) =gkv2*gkv2scale
		} else {
		
			vhalf_h(x)=-81
			gkabar_kap(x) = soma_kap*(1+xdist/100)
			gbar_Kv2like(x) = gkv2
			gkabar_kad(x)=0
		}
	}
	
	insert na16a
		gbar_na16a=gnadend*scale_Na_conduct
		persist_na16a=psoma
		slowdown_na16a = slownotsoma
for (x) {  
			xdist = find_vector_distance_precise(secname(),x)
			xdist=distance(x)
			
			y=30+45*(1-MyExp(-xdist/126))
			
			
			if (y>=25.5) {
			if (y<=44.6) {
				dist_na16a=(y-11)/14
				persist_na16a=psoma
				C1O1v2_na16a=6-6*xdist/200
			} else { if (y<=58.2) {
					dist_na16a=(y-27)/7.3
					persist_na16a=psoma
					C1O1v2_na16a=6-6*xdist/200
					}else { if(y<=65.79) {					
						dist_na16a=(y-44)/3.3 
						persist_na16a=psoma
						C1O1v2_na16a=6-6*xdist/200
						} else{
						dist_na16a=(y-44)/3.3 
						persist_na16a=psoma
						C1O1v2_na16a=distalv}
			}}} else {
			 dist_na16a=(y+2.1)/26.848
			 persist_na16a=psoma
			 C1O1v2_na16a=6-6*xdist/200}
			
		}
	insert kd
		gbar_kd=gkdrapical
		//shift_kdr=shiftkdr
		//scale_kdr=scalekdr
	ena         = potNa
	insert km  // m-type potassium current
		gbar_km    = soma_km
	ek         = -80 
	insert pas

    for (x) {
        xdist=distance(x)

        if (xdist<=100) {
            SpineFactor=1
        } else {
            if (xdist>394) {
                SpineFactor=SpineFactorTuft
            } else {
                SpineFactor=2+(xdist-100)*(SpineFactorTuft-2)/294
            }
        }

        if (xdist<394) {
            Rm=RmTuft+(RmSoma-RmTuft)/(1+MyExp((xdist-DistHalfRm)/SlopeRm))
            Ra=RaTuft+(RaSoma-RaTuft)/(1+MyExp((xdist-DistHalfRa)/SlopeRa))
        } else {
            Rm=RmTuft+(RmSoma-RmTuft)/(1+MyExp((394-DistHalfRm)/SlopeRm))
            Ra=RaTuft+(RaSoma-RaTuft)/(1+MyExp((394-DistHalfRa)/SlopeRa))
        }

        cm(x)=SpineFactor*CmSoma
        g_pas(x)=SpineFactor/Rm
    }
	tmpo2.color(4)
	
	insert kir
        gbar_kir=KirGbar

		
	for (x) {  
		xdist = find_vector_distance_precise(secname(),x)
		xdist=distance(x)
		insert kir
		if (xdist > 100) {            
			gbar_kir=KirGbar
		} else {
			gbar_kir=KirGbar*xdist/100
		}
	}
}
 

// Configure the apical-non-trunk section: insert basic mechanisms  
 
sectype = "apical non-trunk"
forsec apical_non_trunk_list {

	insert ican
		gbar_ican = icangbar
		taur_ican = cantau
		
	//ORIG>>//         apical_h_insert_sig($o1)    // Inserting h-current
	//NDF>>>	apical_caR_caLH_insert($o1) // Inserting Ca++ R-type and Ca++ L-type currents
	for (x) {  
		xdist = find_vector_distance_precise(secname(),x)
		xdist=distance(x)
		insert car
		gcabar_car(x) = 0.1*soma_car

		insert calH
		if (xdist > 50) {            
			gcalbar_calH(x) = 2*soma_caLH    //4.6*soma_caLH
		} else {
			gcalbar_calH(x) = 0.1*soma_caLH               //0.1*soma_caLH
		}
	}
	//<<<NDF
	//NDF>>> apical_caT_insert($o1)      // Inserting Ca++ T-type current
	caT_distal_maxfactor = 4  // ORIG>> maximum cond. factor in dendrites")
	caT_distal_distance  = 350 // ORIG>> distance in dendrites for maximum cond.")
	for (x) {  
		xdist = find_vector_distance_precise(secname(),x)
		xdist=distance(x)
		fr = xdist/caT_distal_distance
		insert cat  
		if (xdist < 100) {
			gcatbar_cat(x) = 0
		} else {
			gcatbar_cat(x) = caT_distal_maxfactor*soma_caT*fr
		} 
    }
	//<<<NDF
	//NDF>>> apical_kca_insert($o1)      // Inserting K(Ca) sAHP and mAHP potassium currents
	kca_distal_maxfactor = 1 // ORIG>> maximum cond. factor in dendrites")
	// ORIG>>//kca_distal_maxfactor = 0 // maximum cond. factor in dendrites
	kca_distal_distance = 200 // ORIG>> distance in dendrites for maximum cond.")
  
	for (x) {  
		xdist = find_vector_distance_precise(secname(),x)
		xdist=distance(x)
		fr = xdist/kca_distal_distance 
		//insert cad    // calsium pump/buffering mechanism
		//taur_cad=20
		insert kca    // slow AHP K++ current
		cac_kca=0.00075 //0.0005
		insert mykca  // medium AHP K++ current

		if (xdist < kca_distal_distance && xdist > 50) {       
			gbar_kca(x) = 5*soma_kca
			gkbar_mykca = 2*mykca_init
		} else {
			gbar_kca(x) = 0.5*soma_kca
			gkbar_mykca = 0.5*mykca_init
		}
	}
	//<<<NDF
	//ORIG>>//        A_insert($o1)               // Inserting A-current
			 
	insert h
	insert kap
	insert Kv2like
	insert kad 
	

	for (x){
		xdist = distance(x)
		if (xdist>500) {xdist=500}
		gbar_h(x) = soma_hbar*(1+(6/5)*xdist/100)
		if (xdist > 100){
			if (xdist>300) {ndist=300} else {ndist=xdist}
			vhalf_h(x)=-81-8*(ndist-100)/200
			gkabar_kad(x) = soma_kad*(1+xdist/100)
			gkabar_kap(x) = 0
			gbar_Kv2like(x) = gkv2*gkv2scale

		} else {
			vhalf_h(x)=-81
			gkabar_kap(x) = soma_kap*(1+xdist/100)
			gbar_Kv2like(x) = gkv2
			gkabar_kad(x) = 0
			
		}
	}
	
	insert na16a
		gbar_na16a    = gnadend*scale_Na_conduct
		persist_na16a=psoma
		slowdown_na16a = slownotsoma

	for (x) {  
			xdist = find_vector_distance_precise(secname(),x)
			xdist=distance(x)
			
			y=30+45*(1-MyExp(-xdist/126))
			
			
						if (y>=25.5) {
			if (y<=44.6) {
				dist_na16a=(y-11)/14
				persist_na16a=psoma
				C1O1v2_na16a=6-6*xdist/200
			} else { if (y<=58.2) {
					dist_na16a=(y-27)/7.3
					persist_na16a=psoma
					C1O1v2_na16a=6-6*xdist/200
					}else { if(y<=65.79) {					
						dist_na16a=(y-44)/3.3 
						persist_na16a=psoma
						C1O1v2_na16a=6-6*xdist/200
						} else{
						dist_na16a=(y-44)/3.3 
						persist_na16a=psoma
						C1O1v2_na16a=distalv}
			}}} else {
			 dist_na16a=(y+2.1)/26.848
			 persist_na16a=psoma
			 C1O1v2_na16a=6-6*xdist/200}
			
		}
	insert kd
		gbar_kd = gkdrapical
		//shift_kdr=shiftkdr
		//scale_kdr=scalekdr
	ena         = potNa
	insert km  // m-type potassium current
		gbar_km    = soma_km
	ek         = -80 

	insert pas

    for (x) {
        xdist=distance(x)

        if (xdist<=100) {
            SpineFactor=1
        } else {
            if (xdist>394) {
                SpineFactor=SpineFactorTuft
            } else {
                SpineFactor=2+(xdist-100)*(SpineFactorTuft-2)/294
            }
        }

        if (xdist<394) {
            Rm=RmTuft+(RmSoma-RmTuft)/(1+MyExp((xdist-DistHalfRm)/SlopeRm))
            Ra=RaTuft+(RaSoma-RaTuft)/(1+MyExp((xdist-DistHalfRa)/SlopeRa))
        } else {
            Rm=RmTuft+(RmSoma-RmTuft)/(1+MyExp((394-DistHalfRm)/SlopeRm))
            Ra=RaTuft+(RaSoma-RaTuft)/(1+MyExp((394-DistHalfRa)/SlopeRa))
        }

        cm(x)=SpineFactor*CmSoma
        g_pas(x)=SpineFactor/Rm
    }

	//ORIG>>//Rm_sigmoid($o1)   // configure Rm along apical trunk
	//ORIG>>//Ra_sigmoid($o1)   // configure Ra along apical trunk
	tmpo2.color(3)
	
	for (x) {  
		xdist = find_vector_distance_precise(secname(),x)
		xdist=distance(x)
		insert kir
		if (xdist > 100) {            
			gbar_kir=KirGbar
		} else {
			gbar_kir=KirGbar*xdist/100
		}
	}
}

//ORIG>>//khoblique_peri_decay($o1)  // Configure the apical oblique dendrites

// Configure the basal dendrites

sectype = "basal tree"
forsec basal_tree_list {
	insert na3dend
	insert nap
		gnabar_nap = 0*.5*0.000014 //flag 
		K_nap = 4.5
		vhalf_nap = -60.4
	insert kap 
		gkabar_kap = 0.0025036
	insert h                
		gbar_h = soma_hbar
	//ORIG>>//ek = -80 
	insert kd
		//shift_kdr=shiftkdr
		//scale_kdr=scalekdr
		gbar_na3dend=gnadend
		gbar_kd=gkdrdend
	ena         = potNa
	
	insert Kv2like
	gbar_Kv2like = gkv2*gkv2scale

	insert pas

    for (x) {
        xdist=distance(x)

        if (xdist<=40) {
            SpineFactor=1
        } else {
            SpineFactor=SpineFactorBasal
        }

        Rm=RmTuft+1*(RmSoma-RmTuft)/(1+MyExp((-DistHalfRm)/SlopeRm))
        Ra=RaTuft+1*(RaSoma-RaTuft)/(1+MyExp((-DistHalfRa)/SlopeRa))
        cm(x)=SpineFactor*CmSoma
        g_pas(x)=SpineFactor/Rm
    }
	
	for (x) {  
		xdist = find_vector_distance_precise(secname(),x)
		xdist=distance(x)
		insert kir
		if (xdist > 40) {            
			gbar_kir=KirGbar
		} else {
			gbar_kir=KirGbar*xdist/40
		}
	}
		
	tmpo2.color(5)
}

//ORIG>>//khbasal_fixed($o1) // Configure basal dendrites         
   
//forsec "soma" { g_pas= 1/Rm_soma} // force Rm at all soma sections

//ORIG>>// forall if (ismembrane("kdr") ) {  
//ORIG>>//           ek         = -80      //-77 
//ORIG>>//      }



forall if(ismembrane("ca_ion")) {
	eca =140
	ion_style("ca_ion",0,1,0,0,0)
	vshift_ca = 0
}

maximum_segment_length=75
//freq = 150      // Hz, frequency at which AC length constant will be computed
freq = 100      // Hz, frequency at which AC length constant will be computed
d_lambda = 0.1

func lambda_f() { local i, x1, x2, d1, d2, lam
        if (n3d() < 2) {
                return 1e5*sqrt(diam/(4*PI*$1*Ra*cm))
        }
// above was too inaccurate with large variation in 3d diameter
// so now we use all 3-d points to get a better approximate lambda
        x1 = arc3d(0)
        d1 = diam3d(0)
        lam = 0
        for i=1, n3d()-1 {
                x2 = arc3d(i)
                d2 = diam3d(i)
                lam += (x2 - x1)/sqrt(d1 + d2)
                x1 = x2   d1 = d2
        }
        //  length of the section in units of lambda
        lam *= sqrt(2) * 1e-5*sqrt(4*PI*$1*Ra*cm)

        return L/lam
}


  
if(balance) forall {
      for (x) {
        if (ismembrane("na_ion") && ismembrane("ca_ion") && ismembrane("Ca_ion") && (ismembrane("k_ion"))) {
            e_pas(x)=(ina(x)+ik(x)+ica(x)+iCa(x)+g_pas(x)*v(x))/g_pas(x) 
        } else if (ismembrane("na_ion") && ismembrane("ca_ion") && (ismembrane("k_ion"))){
            e_pas(x)=(ina(x)+ik(x)+ica(x)+g_pas(x)*v(x))/g_pas(x) 
        } else if (ismembrane("na_ion") && (ismembrane("k_ion"))) {
            e_pas(x)=(ina(x)+ik(x)+g_pas(x)*v(x))/g_pas(x)
          printf("Section %s ina: %g ik: %g\n", secname(), ina(x), ik(x))
          psection()
        } else {
          print "simply assigning v(x)"
          e_pas(x)=v(x)
        }
      fcurrent()
    }
  } 
  
forall{
	for(x){
		e_pas=Epas}
		}
		
if(geomnseg) {forall {
    nseg = int((L/(d_lambda*lambda_f(freq))+0.9)/2)*2 + 1
}
}

forall {
	for(x){
		insert cal4
		DCa_cal4=0.22
		cao0_ca_ion=2
		ip3i_cal4=0.16e-3
		
		if(ismembrane("ican")){
				concrelease_ican=1
				}
				
		//if(ismembrane("na16a")){
			//	dist_na16a=12.5
			//	}
		
		}
	}
	
forsec "soma" {

		for (x) {
			rdist=distance(x)
			alpha_cal4(x)=gip3*(0.75+.25*MyExp(-rdist/100))
			print x, rdist, alpha_cal4(x)
		}
	
	}
	
forsec apical_trunk_list {
	
		for (x) {
			rdist=distance(x)
			alpha_cal4(x)=gip3*(0.75+.25*MyExp(-rdist/100))
			print x, rdist, alpha_cal4(x)
		}
	
	}
	
forsec apical_non_trunk_list {

		for (x) {
			rdist=distance(x)
			alpha_cal4(x)=gip3*(0.75+.25*MyExp(-rdist/100))
			print x, rdist, alpha_cal4(x)
		}
	
	}
	
forsec basal_tree_list {
	
		for (x) {
			rdist=distance(x)
			alpha_cal4(x)=gip3*(0.75+.25*MyExp(-rdist/100))
			print x, rdist, alpha_cal4(x)
		}
	
	}

		
if(geomnseg) {forall {
    nseg = int((L/(d_lambda*lambda_f(freq))+0.9)/2)*2 + 1 //1
}
}