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pYPKpw.gb
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LOCUS pYPKpw 5603 bp DNA circular SYN 01-JAN-1980
DEFINITION pcr_product_866_pYPKpwF (39-mer)_865_pYPKpwR (39-mer)
cSEGUID_WeyovdMmqwA4bc9EqEwUDmbo3Lg.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE
ORGANISM .
.
COMMENT Annotated with pLannotate v1.2.0
pydna cdseguid=xj7evEO4h83-RGFeHu8q15tL_Qs 2024-06-11T18:53:45
FEATURES Location/Qualifiers
primer_bind complement(96..124)
/label="865_pYPKpwR"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
primer_bind 144..173
/label="866_pYPKpwF"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
promoter complement(292..311)
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="44.4"
/fragment="True"
/other="promoter"
/locus_tag="T5 promoter (fragment)"
/label="T5 promoter (fragment)"
/ApEinfo_label="T5 promoter (fragment)"
/ApEinfo_fwdcolor="#EE92FE"
/ApEinfo_revcolor="#EE92FE"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
misc_feature 312..411
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="70.9"
/fragment="True"
/other="misc_feature"
/locus_tag="bom (fragment)"
/label="bom (fragment)"
/ApEinfo_label="bom (fragment)"
/ApEinfo_fwdcolor="#ccff6b"
/ApEinfo_revcolor="#ccff6b"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
rep_origin complement(597..1185)
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="rep_origin"
/locus_tag="ori"
/label="ori"
/ApEinfo_label="ori"
/ApEinfo_fwdcolor="#2D64FE"
/ApEinfo_revcolor="#2D64FE"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
ncRNA 1157..1261
/note="pLannotate"
/label="RNAI"
/database="Rfam"
/identity="100.0"
/match_length="102.9"
/fragment="False"
/other="ncRNA"
promoter complement(1435..1453)
/note="pLannotate"
/label="AmpR promoter (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="29.7"
/fragment="True"
/other="promoter"
gene complement(1456..2259)
/gene="ura3"
CDS complement(1456..2259)
/gene="ura3"
/codon_start=1
/transl_table=11
/product="orotidine-5'-phosphate decarboxylase"
/protein_id="BAE72675.1"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
CDS complement(1456..2259)
/note="pLannotate"
/label="URA3"
/database="snapgene"
/identity="99.8"
/match_length="100.0"
/fragment="False"
/other="CDS"
misc complement(1456..2259)
/label="URA3"
promoter complement(2260..2475)
/note="pLannotate"
/label="URA3 promoter"
/database="snapgene"
/identity="99.5"
/match_length="100.0"
/fragment="False"
/other="promoter"
promoter complement(2486..2509)
/note="pLannotate"
/label="tet promoter (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="82.8"
/fragment="True"
/other="promoter"
misc_feature complement(2492..2528)
/note="pLannotate"
/label="CEN/ARS (fragment)"
/database="snapgene"
/identity="97.3"
/match_length="3.2"
/fragment="True"
/other="misc_feature"
CDS 2500..2925
/note="pLannotate"
/label="RAF1 (fragment)"
/database="swissprot"
/identity="93.0"
/match_length="78.5"
/fragment="True"
/other="CDS"
rep_origin complement(3079..3959)
/note="pLannotate"
/label="2μ ori"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="rep_origin"
rep_origin complement(3970..4011)
/note="pLannotate"
/label="M13 ori (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="8.2"
/fragment="True"
/other="rep_origin"
gene 4185..5045
/gene="Amp"
CDS 4185..5045
/gene="Amp"
/codon_start=1
/transl_table=11
/product="ampicillin resistant protein"
/protein_id="BAE72674.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS 4185..5045
/note="pLannotate"
/label="AmpR"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="CDS"
CDS 4188..4846
/label="AmpR"
promoter complement(4851..4955)
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="promoter"
/locus_tag="AmpR promoter"
/label="AmpR promoter"
/ApEinfo_label="AmpR promoter"
/ApEinfo_fwdcolor="#00891F"
/ApEinfo_revcolor="#00891F"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
ncRNA 4909..5013
/note="pLannotate"
/database="Rfam"
/identity="100.0"
/match_length="102.9"
/fragment="False"
/other="ncRNA"
/locus_tag="RNAI"
/label="RNAI"
/ApEinfo_label="RNAI"
/ApEinfo_fwdcolor="#00D308"
/ApEinfo_revcolor="#00D308"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
CDS 5046..5081
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="18.8"
/fragment="True"
/other="CDS"
/locus_tag="rop (fragment)"
/label="rop (fragment)"
/ApEinfo_label="rop (fragment)"
/ApEinfo_fwdcolor="#A600FA"
/ApEinfo_revcolor="#A600FA"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
misc_feature 5183..5232
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="35.5"
/fragment="True"
/other="misc_feature"
/locus_tag="bom (fragment)(1)"
/label="bom (fragment)(1)"
/ApEinfo_label="bom (fragment)"
/ApEinfo_fwdcolor="#ccff6b"
/ApEinfo_revcolor="#ccff6b"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
terminator complement(5300..5327)
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="70.0"
/fragment="True"
/other="terminator"
/locus_tag="fd terminator (fragment)"
/label="fd terminator (fragment)"
/ApEinfo_label="fd terminator (fragment)"
/ApEinfo_fwdcolor="#2D64FE"
/ApEinfo_revcolor="#2D64FE"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
misc_feature 5328..5360
/locus_tag="from Ec chromosome"
/label="from Ec chromosome"
/ApEinfo_label="from Ec chromosome"
/ApEinfo_fwdcolor="cyan"
/ApEinfo_revcolor="green"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
rep_origin complement(3159..4039)
/note="pLannotate"
/label="2μ ori"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="rep_origin"
CDS complement(4185..5045)
/note="pLannotate"
/label="AmpR"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="CDS"
rep_origin complement(792..1380)
/note="pLannotate"
/label="ori"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="rep_origin"
promoter complement(5046..5150)
/note="pLannotate"
/label="AmpR promoter"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="promoter"
CDS complement(1536..2339)
/note="pLannotate"
/label="URA3"
/database="snapgene"
/identity="99.6"
/match_length="100.0"
/fragment="False"
/other="CDS"
promoter complement(2340..2555)
/note="pLannotate"
/label="URA3 promoter"
/database="snapgene"
/identity="99.5"
/match_length="100.0"
/fragment="False"
/other="promoter"
CDS complement(join(5559..5603,1..426))
/note="pLannotate"
/label="crp (fragment)"
/database="swissprot"
/identity="73.3"
/match_length="74.8"
/fragment="True"
/other="CDS"
CDS 2580..3005
/note="pLannotate"
/label="RAF1 (fragment)"
/database="swissprot"
/identity="93.0"
/match_length="78.5"
/fragment="True"
/other="CDS"
misc_feature 507..606
/note="pLannotate"
/label="bom (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="70.9"
/fragment="True"
/other="misc_feature"
ncRNA 1235..1339
/note="pLannotate"
/label="RNAI"
/database="Rfam"
/identity="100.0"
/match_length="102.9"
/fragment="False"
/other="ncRNA"
promoter complement(2566..2589)
/note="pLannotate"
/label="tet promoter (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="82.8"
/fragment="True"
/other="promoter"
terminator complement(5495..5522)
/note="pLannotate"
/label="fd terminator (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="70.0"
/fragment="True"
/other="terminator"
misc_feature 5378..5427
/note="pLannotate"
/label="bom (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="35.5"
/fragment="True"
/other="misc_feature"
promoter complement(487..506)
/note="pLannotate"
/label="T5 promoter (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="44.4"
/fragment="True"
/other="promoter"
CDS 5241..5276
/note="pLannotate"
/label="rop (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="18.8"
/fragment="True"
/other="CDS"
rep_origin complement(4050..4091)
/note="pLannotate"
/label="M13 ori (fragment)"
/database="snapgene"
/identity="100.0"
/match_length="8.2"
/fragment="True"
/other="rep_origin"
misc_feature complement(2572..2608)
/note="pLannotate"
/label="CEN/ARS (fragment)"
/database="snapgene"
/identity="97.3"
/match_length="3.2"
/fragment="True"
/other="misc_feature"
ORIGIN
1 gttctgatcc tcgagcatct taagaattcg tcccacggtt tgtctagagc agccgacaat
61 ctggccaatt tcctgacggg taattttgat ttgcatgccg tccgggtgag tcatagcgtc
121 tggtgacgtc atgcgcatga tatcttcaca ggcggttttc gcacgtaccc atgcgctacg
181 ttcctggccc tcttcaaaca ggcccagttc gccaataaaa tcaccctgat tcagatagga
241 gaggatcatt tctttaccct cttcgtcttt gatcagcact gccacagagc ctttaacgat
301 gtagtacagc gtttccgctt tttcaccctg gtgaataagc gtgctcttgg atgggtactt
361 atgaatgtgg caatgagaca agaaccattc gagagtagga tccgtttgag gtttaccaag
421 taccataaga tccttaaatt tttattatct agctagatga taatattata tcaagaattg
481 tacctgaaag caaataaatt ttttatctgg cttaactatg cggcatcaga gcagattgta
541 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
601 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
661 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
721 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
901 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
961 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
1021 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
1081 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
1141 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
1201 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
1261 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
1321 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
1381 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
1441 gggattttgg tcatgagggg taataactga tataattaaa ttgaagctct aatttgtgag
1501 tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc gcatcttctc
1561 aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc atcccttccc
1621 tttgcaaata gtcctcttcc aacaataata atgtcagatc ctgtagagac cacatcatcc
1681 acggttctat actgttgacc caatgcgtct cccttgtcat ctaaacccac accgggtgtc
1741 ataatcaacc aatcgtaacc ttcatctctt ccacccatgt ctctttgagc aataaagccg
1801 ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac ccttagtata ttctccagta
1861 gatagggagc ccttgcatga caattctgct aacatcaaaa ggcctctagg ttcctttgtt
1921 acttcttctg ccgcctgctt caaaccgcta acaatacctg ggcccaccac accgtgtgca
1981 ttcgtaatgt ctgcccattc tgctattctg tatacacccg cagagtactg caatttgact
2041 gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta cttggcggat
2101 aatgccttta gcggcttaac tgtgccctcc atggaaaaat cagtcaaaat atccacatgt
2161 gtttttagta aacaaatttt gggacctaat gcttcaacta actccagtaa ttccttggtg
2221 gtacgaacat ccaatgaagc acacaagttt gtttgctttt cgtgcatgat attaaatagc
2281 ttggcagcaa caggactagg atgagtagca gcacgttcct tatatgtagc tttcgacatg
2341 atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt gggttaagaa tactgggcaa
2401 tttcatgttt cttcaacact acatatgcgt atatatacca atctaagtct gtgctccttc
2461 cttcgttctt ccttctgttc ggagattacc gaatcaaaaa aatttcaaag aaaccgaaat
2521 caaaaaaaag aataaaaaaa aaatgatgaa ttgaattgaa aagctagctt atcgatgata
2581 agctgtcaaa gatgagaatt aattccacgg actatagact atactagata ctccgtctac
2641 tgtacgatac acttccgctc aggtccttgt cctttaacga ggccttacca ctcttttgtt
2701 actctattga tccagctcag caaaggcagt gtgatctaag attctatctt cgcgatgtag
2761 taaaactagc tagaccgaga aagagactag aaatgcaaaa ggcacttcta caatggctgc
2821 catcattatt atccgatgtg acgctgcagc ttctcaatga tattcgaata cgctttgagg
2881 agatacagcc taatatccga caaactgttt tacagattta cgatcgtact tgttacccat
2941 cattgaattt tgaacatccg aacctgggag ttttccctga aacagatagt atatttgaac
3001 ctgtataata atatatagtc tagcgcttta cggaagacaa tgtatgtatt tcggttcctg
3061 gagaaactat tgcatctatt gcataggtaa tcttgcacgt cgcatccccg gttcattttc
3121 tgcgtttcca tcttgcactt caatagcata tctttgttaa cgaagcatct gtgcttcatt
3181 ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
3241 tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
3301 catttttgta aaacaaaaat gcaacgcgac gagagcgcta atttttcaaa caaagaatct
3361 gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat
3421 ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc
3481 atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt
3541 tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat
3601 aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt
3661 ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg
3721 tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct
3781 tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat
3841 tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa
3901 cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta
3961 ggttatatag ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg
4021 tggaagcggt attcgcaatg ggaagctcca ccccggttga taatcagaaa agccccaaaa
4081 acaggaagat tattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
4141 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
4201 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
4261 gtcgtgtaga taactacgat acgggagcgc ttaccatctg gccccagtgc tgcaatgata
4321 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
4381 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
4441 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tggcattgct
4501 acaggcatcg tggtgtcact ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
4561 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
4621 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
4681 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
4741 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
4801 atacgggata atagtgtatc acatagcaga actttaaaag tgctcatcat tggaaaacgt
4861 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
4921 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
4981 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
5041 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
5101 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
5161 cgaaaagtgc cacctgctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
5221 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
5281 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
5341 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
5401 agcagattgt actgagagtg caccatagat cctgaggatc ggggtgataa atcagtctgc
5461 gccacatcgg gggaaacaaa atggcgcgag atctaaaaaa aaaggctcca aaaggagcct
5521 ttcgcgctac caggtaacgc gccactccga cgggattaac gagtgccgta aacgacgatg
5581 gttttaccgt gtgcggagat cag
//