-
Notifications
You must be signed in to change notification settings - Fork 0
/
Copy pathpUG35.gb
executable file
·266 lines (266 loc) · 15.8 KB
/
pUG35.gb
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
LOCUS AF298787_1 6231 bp DNA circular UNK 05-APR-2021
DEFINITION C-terminal GFP fusion vector pUG35, complete sequence
cSEGUID_j5HPv0XMvCKlAodFVKKzgcWzPvk.
ACCESSION AF298787
VERSION AF298787.1 GI:11344888
KEYWORDS .
SOURCE C-terminal GFP fusion vector pUG35
ORGANISM C-terminal GFP fusion vector pUG35 other sequences; artificial
sequences; vectors.
REFERENCE 1 (bases 1 to 6231)
AUTHORS Gueldener,U. and Hegemann,J.H.
TITLE A second generation of GFP-vectors for subcelluar localization
studies in budding yeast
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6231)
AUTHORS Gueldener,U. and Hegemann,J.H.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) Institut fuer Mikrobiologie,
Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf
40225, Germany
COMMENT ApEinfo:methylated:0
pydna cdseguid=4cVPUTb2zz_rtTp5F2mci2l09rQ 2024-06-11T18:53:37
FEATURES Location/Qualifiers
source 1..6231
/organism="C-terminal GFP fusion vector pUG35"
/mol_type="other DNA"
/db_xref="taxon:142955"
/note="derived from Saccharomyces cerevisiae"
/locus_tag="derived from Saccharomyces cerevisiae"
/label="derived from Saccharomyces cerevisiae"
/ApEinfo_label="derived from Saccharomyces cerevisiae"
/ApEinfo_fwdcolor="pink"
/ApEinfo_revcolor="pink"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
/ApEinfo_hidden=""
gene 417..1220
/gene="URA3"
/locus_tag="URA3"
/label="URA3"
/ApEinfo_label="URA3"
/ApEinfo_fwdcolor="pink"
/ApEinfo_revcolor="pink"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
CDS 417..1220
/gene="URA3"
/codon_start=1
/transl_table=11
/product="orotidine-5'-phosphate decarboxylase"
/protein_id="AAG34528.1"
/db_xref="GI:11344889"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
/locus_tag="orotidine-5'-phosphate decarboxylase"
/label="orotidine-5'-phosphate decarboxylase"
/ApEinfo_label="orotidine-5'-phosphate decarboxylase"
/ApEinfo_fwdcolor="pink"
/ApEinfo_revcolor="pink"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
terminator 2004..2262
/note="from CYC1"
/locus_tag="from CYC1"
/label="from CYC1"
/ApEinfo_label="from CYC1"
/ApEinfo_fwdcolor="pink"
/ApEinfo_revcolor="pink"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
gene complement(2271..2987)
/gene="EGFP3"
/locus_tag="EGFP3"
/label="EGFP3"
/ApEinfo_label="EGFP3"
/ApEinfo_fwdcolor="#10c00e"
/ApEinfo_revcolor="#11c01b"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
CDS complement(2271..2987)
/gene="EGFP3"
/codon_start=1
/transl_table=11
/product="enhanced green fluorescent protein 3"
/protein_id="AAG34529.1"
/db_xref="GI:11344890"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
/locus_tag="enhanced green fluorescent protein 3"
/label="enhanced green fluorescent protein 3"
/ApEinfo_label="enhanced green fluorescent protein 3"
/ApEinfo_fwdcolor="#09c016"
/ApEinfo_revcolor="#11c006"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
promoter 3051..3443
/note="from MET25"
/locus_tag="from MET25"
/label="from MET25"
/ApEinfo_label="from MET25"
/ApEinfo_fwdcolor="pink"
/ApEinfo_revcolor="pink"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
rep_origin 3882..3954
/locus_tag="rep_origin"
/label="rep_origin"
/ApEinfo_label="rep_origin"
/ApEinfo_fwdcolor="pink"
/ApEinfo_revcolor="pink"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
gene complement(4657..5517)
/gene="AmpR"
/locus_tag="AmpR"
/label="AmpR"
/ApEinfo_label="AmpR"
/ApEinfo_fwdcolor="pink"
/ApEinfo_revcolor="pink"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
CDS complement(4657..5517)
/gene="AmpR"
/codon_start=1
/transl_table=11
/product="Protocol for ampicillin stock solution resistance
protein"
/protein_id="AAG34530.1"
/db_xref="GI:11344891"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
/locus_tag="Protocol for ampicillin stock solution
resistance protein"
/label="Protocol for ampicillin stock solution resistance
protein"
/ApEinfo_label="Protocol for ampicillin stock solution
resistance protein"
/ApEinfo_fwdcolor="pink"
/ApEinfo_revcolor="pink"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
misc_feature 5656..6170
/note="CEN6/ARSH4"
/locus_tag="CEN6/ARSH4"
/label="CEN6/ARSH4"
/ApEinfo_label="CEN6/ARSH4"
/ApEinfo_fwdcolor="#ccff6b"
/ApEinfo_revcolor="#ccff6b"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
1981 acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg agcgtcccaa
2041 aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa
2101 aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata
2161 aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg
2221 gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaccag ttatttgtac
2281 aattcatcca taccatgggt aataccagca gcagtaacaa attctaacaa gaccatgtgg
2341 tctctctttt cgtttggatc tttggataag gcagattgag tggataagta atggttgtct
2401 ggtaacaaga ctggaccatc accaattgga gtattttgtt gataatggtc agctaattga
2461 acagaaccat cttcaatgtt gtgtctaatt ttgaagttaa ctttgatacc attcttttgt
2521 ttgtcagcca tgatgtaaac attgtgagag ttatagttgt attccaattt gtgacctaaa
2581 atgttaccat cttctttaaa atcaatacct tttaattcga ttctattaac taaggtatca
2641 ccttcaaact tgacttcagc tctggtcttg tagttaccgt catctttgaa aaaaatagtt
2701 ctttcttgaa cataaccttc tggcatggca gacttgaaaa agtcatgttg tttcatatga
2761 tctgggtatc tagcaaaaca ttgaacacca taaccgaaag tagtgactaa ggttggccat
2821 ggaactggca atttaccagt agtacaaata aattttaagg tcaatttacc gtaagtagca
2881 tcaccttcac cttcaccgga gacagaaaat ttgtgaccat taacatcacc atctaattca
2941 accaaaattg ggacaacacc agtgaataat tcttcacctt tagacatgtc gaggtcgacg
3001 gtatcgataa gcttgatatc gaattcctgc agcccggggg atccactagt tctagagtat
3061 ggatgggggt aatagaattg tatctatgta tctgacgacc ctgtattaca cgaaggaaat
3121 agtagatgaa aagacaagag agcaagaaaa aggaaaaact tcctttctaa cagacgcttt
3181 acttaacctt atatatatct tatttttttc atcgagcgtg ttcaattgga caaggtgcca
3241 ccttttcgac acttcggtta ttatgttaca cagttttcat gaggatggcg ccttgactaa
3301 cttaattagt catttgccaa ccaccacagt tccccaatat cgacagcttc acgtgccatt
3361 tgccattttg cgatcatgtg acctcaagag aaaaagcaaa aaataatgag agctaaggga
3421 ttcgaaccct tgcatccgga gctccagctt ttgttccctt tagtgagggt taattgcgcg
3481 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
3541 acacaacata ggagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgaggta
3601 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
3661 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
3721 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
3781 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
3841 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
3901 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
3961 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
4021 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
4081 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
4141 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
4201 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
4261 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
4321 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
4381 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
4441 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
4501 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
4561 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
4621 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
4681 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
4741 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
4801 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
4861 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
4921 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
4981 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
5041 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
5101 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
5161 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
5221 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
5281 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
5341 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
5401 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
5461 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
5521 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
5581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
5641 gccacctggg tccttttcat cacgtgctat aaaaataatt ataatttaaa ttttttaata
5701 taaatatata aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt
5761 tttccgaaga tgtaaaagac tctaggggga tcgccaacaa atactacctt ttatcttgct
5821 cttcctgctc tcaggtatta atgccgaatt gtttcatctt gtctgtgtag aagaccacac
5881 acgaaaatcc tgtgatttta cattttactt atcgttaatc gaatgtatat ctatttaatc
5941 tgcttttctt gtctaataaa tatatatgta aagtacgctt tttgttgaaa ttttttaaac
6001 ctttgtttat ttttttttct tcattccgta actcttctac cttctttatt tactttctaa
6061 aatccaaata caaaacataa aaataaataa acacagagta aattcccaaa ttattccatc
6121 attaaaagat acgaggcgcg tgtaagttac aggcaagcga tccgtcctaa gaaaccatta
6181 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt c
//