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pTA1.gb
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LOCUS pTA1 6175 bp DNA circular UNK 23-MAY-2024
DEFINITION pTA1 = ampR pBR 2µ LEU2 CRPΔ µ828.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE
ORGANISM . .
.
COMMENT Annotated with pLannotate v1.2.0. pydna cSEGUID
_E-9A37sZOIs9RFYPb3ENzud6FI 2022-11-08T18:16:15
ApEinfo:methylated:1
pydna cdseguid=MtltqcR0b0kVX_FJ4BYL-PjEAfg 2024-06-11T18:53:46
FEATURES Location/Qualifiers
primer_bind 1..29
/locus_tag="577_crp585-557"
/label="577_crp585-557"
/ApEinfo_label="577_crp585-557"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
primer_bind 1..16
/locus_tag="978_Crp.FW"
/label="978_Crp.FW"
/ApEinfo_label="978_Crp.FW"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
primer_bind complement(96..124)
/locus_tag="865_pYPKpwR"
/label="865_pYPKpwR"
/ApEinfo_label="865_pYPKpwR"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
primer_bind 144..173
/locus_tag="866_pYPKpwF"
/label="866_pYPKpwF"
/ApEinfo_label="866_pYPKpwF"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
primer_bind complement(357..385)
/locus_tag="578_crp42-70"
/label="578_crp42-70"
/ApEinfo_label="578_crp42-70"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
primer_bind complement(369..385)
/locus_tag="977_Crp.REV"
/label="977_Crp.REV"
/ApEinfo_label="977_Crp.REV"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
primer_bind 446..470
/locus_tag="1113_Amp.fw.nw"
/label="1113_Amp.fw.nw"
/ApEinfo_label="1113_Amp.fw.nw"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
gene 547..1407
/gene="AmpR"
/locus_tag="AmpR"
/label="AmpR"
/ApEinfo_label="AmpR"
/ApEinfo_fwdcolor="#ff797d"
/ApEinfo_revcolor="#ff102f"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
CDS 547..1407
/gene="AmpR"
/codon_start=1
/transl_table=11
/product="Protocol for ampicillin stock solution resistance
protein"
/protein_id="AAG34530.1"
/db_xref="GI:11344891"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
/locus_tag="Protocol for ampicillin stock solution
resistance protein"
/label="Protocol for ampicillin stock solution resistance
protein"
/ApEinfo_label="Protocol for ampicillin stock solution
resistance protein"
/ApEinfo_fwdcolor="#e9d024"
/ApEinfo_revcolor="#e9d024"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
primer_bind complement(1436..1457)
/locus_tag="987_Amp.REV"
/label="987_Amp.REV"
/ApEinfo_label="987_Amp.REV"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
repeat_region complement(1565..1602)
/note="corresponds to one of the 38bp repeats found in Tn3
(bp 1-38 and complement (4920-4957))"
/rpt_type=inverted
/locus_tag="corresponds to one of the 38bp repeats found in
Tn3 (bp 1-38 and complement (4920-4957))"
/label="corresponds to one of the 38bp repeats found in Tn3
(bp 1-38 and complement (4920-4957))"
/ApEinfo_label="corresponds to one of the 38bp repeats
found in Tn3 (bp 1-38 and complement (4920-4957))"
/ApEinfo_fwdcolor="#0dfff7"
/ApEinfo_revcolor="#0dfff7"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
old_sequence complement(2020..2021)
/note="revision according to (17)"
/citation=[2]
/citation=[17]
/replace="at"
/locus_tag="revision according to (17)"
/label="revision according to (17)"
/ApEinfo_label="revision according to (17)"
/ApEinfo_fwdcolor="#00DF87"
/ApEinfo_revcolor="#00DF87"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
old_sequence complement(2020)
/note="revision according to (16)"
/citation=[2]
/citation=[16]
/citation=[17]
/replace="t"
/locus_tag="revision according to (16)"
/label="revision according to (16)"
/ApEinfo_label="revision according to (16)"
/ApEinfo_fwdcolor="#00D95A"
/ApEinfo_revcolor="#00D95A"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
old_sequence complement(2021)
/citation=[17]
/locus_tag="old_sequence"
/label="old_sequence"
/ApEinfo_label="old_sequence"
/ApEinfo_fwdcolor="#008065"
/ApEinfo_revcolor="#008065"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
rep_origin complement(2215)
/locus_tag="rep_origin"
/label="rep_origin"
/ApEinfo_label="rep_origin"
/ApEinfo_fwdcolor="#999999"
/ApEinfo_revcolor="#999999"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
misc_binding complement(2303..2311)
/bound_moiety="dnaA"
/locus_tag="misc_binding"
/label="misc_binding"
/ApEinfo_label="misc_binding"
/ApEinfo_fwdcolor="#ff4809"
/ApEinfo_revcolor="#ff4809"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
misc_feature 2336..2399
/note="L-strand Y effector site"
/citation=[5]
/locus_tag="L-strand Y effector site"
/label="L-strand Y effector site"
/ApEinfo_label="L-strand Y effector site"
/ApEinfo_fwdcolor="#7eff74"
/ApEinfo_revcolor="#7eff74"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
repeat_region 2501..2505
/note="gamma-delta insertion target sequence"
/rpt_type=direct
/locus_tag="gamma-delta insertion target sequence"
/label="gamma-delta insertion target sequence"
/ApEinfo_label="gamma-delta insertion target sequence"
/ApEinfo_fwdcolor="#0dfff7"
/ApEinfo_revcolor="#0dfff7"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
misc_feature complement(2583..2739)
/note="H-strand Y effector site"
/citation=[5]
/locus_tag="H-strand Y effector site"
/label="H-strand Y effector site"
/ApEinfo_label="H-strand Y effector site"
/ApEinfo_fwdcolor="#7eff74"
/ApEinfo_revcolor="#7eff74"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
CDS complement(2644..2835)
/codon_start=1
/transl_table=11
/product="ROP protein"
/protein_id="AAB59736.1"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
/locus_tag="ROP protein"
/label="ROP protein"
/ApEinfo_label="ROP protein"
/ApEinfo_fwdcolor="#e9d024"
/ApEinfo_revcolor="#e9d024"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
old_sequence complement(2835..2836)
/citation=[17]
/locus_tag="old_sequence(1)"
/label="old_sequence(1)"
/ApEinfo_label="old_sequence"
/ApEinfo_fwdcolor="#13AAFE"
/ApEinfo_revcolor="#13AAFE"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
misc_difference complement(2836..2837)
/note="conflict"
/citation=[23]
/replace="caa"
/locus_tag="conflict"
/label="conflict"
/ApEinfo_label="conflict"
/ApEinfo_fwdcolor="#3A93FE"
/ApEinfo_revcolor="#3A93FE"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
regulatory complement(2840..2845)
/regulatory_class="ribosome_binding_site"
/locus_tag="regulatory:ribosome_binding_site"
/label="regulatory:ribosome_binding_site"
/ApEinfo_label="regulatory:ribosome_binding_site"
/ApEinfo_fwdcolor="#2D64FE"
/ApEinfo_revcolor="#2D64FE"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
regulatory complement(2841..2845)
/note="Shine-Dalgarno sequence"
/regulatory_class="ribosome_binding_site"
/locus_tag="Shine-Dalgarno sequence"
/label="Shine-Dalgarno sequence"
/ApEinfo_label="Shine-Dalgarno sequence"
/ApEinfo_fwdcolor="#00891F"
/ApEinfo_revcolor="#00891F"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
old_sequence complement(2857..2858)
/citation=[2]
/citation=[22]
/locus_tag="old_sequence(2)"
/label="old_sequence(2)"
/ApEinfo_label="old_sequence"
/ApEinfo_fwdcolor="#00C6C3"
/ApEinfo_revcolor="#00C6C3"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
misc_difference complement(2858..2859)
/note="conflict"
/citation=[23]
/replace="att"
/locus_tag="conflict(1)"
/label="conflict(1)"
/ApEinfo_label="conflict"
/ApEinfo_fwdcolor="#00D0FA"
/ApEinfo_revcolor="#00D0FA"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
primer_bind 2875..2893
/locus_tag="1196_Pbr.FW"
/label="1196_Pbr.FW"
/ApEinfo_label="1196_Pbr.FW"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
primer_bind complement(2881..2894)
/locus_tag="1195_Pbr.REV"
/label="1195_Pbr.REV"
/ApEinfo_label="1195_Pbr.REV"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
primer_bind 2925..2943
/locus_tag="984_Micron.FW"
/label="984_Micron.FW"
/ApEinfo_label="984_Micron.FW"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
primer_bind complement(4491..4508)
/locus_tag="983_Micron.REV"
/label="983_Micron.REV"
/ApEinfo_label="983_Micron.REV"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
primer_bind 4539..4556
/locus_tag="980_Leu2.FW"
/label="980_Leu2.FW"
/ApEinfo_label="980_Leu2.FW"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
promoter 4541..4948
/gene="S. cerevisiae LEU2"
/locus_tag="LEU2 promoter"
/label="LEU2 promoter"
/ApEinfo_label="LEU2 promoter"
/ApEinfo_fwdcolor="#00D0FA"
/ApEinfo_revcolor="#00D0FA"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
promoter 4541..4948
/note="pLannotate"
/database="snapgene"
/identity="99.5"
/match_length="100.0"
/fragment="False"
/other="promoter"
/locus_tag="LEU2 promoter(1)"
/label="LEU2 promoter(1)"
/ApEinfo_label="LEU2 promoter"
/ApEinfo_fwdcolor="#7160FE"
/ApEinfo_revcolor="#7160FE"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
variation complement(4750..4751)
/locus_tag="New Feature"
/label="New Feature"
/ApEinfo_label="New Feature"
/ApEinfo_fwdcolor="cyan"
/ApEinfo_revcolor="green"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
CDS 4949..6043
/codon_start=1
/gene="S. cerevisiae LEU2"
/product="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/note="yeast auxotrophic marker"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA"
/locus_tag="LEU2"
/label="LEU2"
/ApEinfo_label="LEU2"
/ApEinfo_fwdcolor="#00C6C3"
/ApEinfo_revcolor="#00C6C3"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
CDS 4949..6043
/note="pLannotate"
/database="snapgene"
/identity="99.5"
/match_length="100.0"
/fragment="False"
/other="CDS"
/locus_tag="LEU2(1)"
/label="LEU2(1)"
/ApEinfo_label="LEU2"
/ApEinfo_fwdcolor="#4856FE"
/ApEinfo_revcolor="#4856FE"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {} 0} width 5 offset 0"
primer_bind complement(6126..6146)
/locus_tag="979_Leu2.REV"
/label="979_Leu2.REV"
/ApEinfo_label="979_Leu2.REV"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
rep_origin complement(3162..4508)
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="rep_origin"
/locus_tag="2μ ori"
/label="2μ ori"
/ApEinfo_label="2μ ori"
/ApEinfo_fwdcolor="#999999"
/ApEinfo_revcolor="#999999"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
rep_origin 1628..2216
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="rep_origin"
/locus_tag="ori"
/label="ori"
/ApEinfo_label="ori"
/ApEinfo_fwdcolor="#999999"
/ApEinfo_revcolor="#999999"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
CDS complement(2644..2835)
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="CDS"
/locus_tag="rop"
/label="rop"
/ApEinfo_label="rop"
/ApEinfo_fwdcolor="#e9d024"
/ApEinfo_revcolor="#e9d024"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
misc_feature complement(2402..2542)
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="misc_feature"
/locus_tag="bom"
/label="bom"
/ApEinfo_label="bom"
/ApEinfo_fwdcolor="#7eff74"
/ApEinfo_revcolor="#7eff74"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
CDS 4949..6043
/note="pLannotate"
/database="snapgene"
/identity="99.5"
/match_length="100.0"
/fragment="False"
/other="CDS"
/locus_tag="LEU2(2)"
/label="LEU2(2)"
/ApEinfo_label="LEU2"
/ApEinfo_fwdcolor="#e9d024"
/ApEinfo_revcolor="#e9d024"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
promoter 425..516
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="100.0"
/fragment="False"
/other="promoter"
/locus_tag="AmpR promoter"
/label="AmpR promoter"
/ApEinfo_label="AmpR promoter"
/ApEinfo_fwdcolor="#346ee0"
/ApEinfo_revcolor="#346ee0"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
CDS 517..1377
/note="pLannotate"
/database="snapgene"
/identity="99.8"
/match_length="100.0"
/fragment="False"
/other="CDS"
/locus_tag="AmpR(1)"
/label="AmpR(1)"
/ApEinfo_label="AmpR"
/ApEinfo_fwdcolor="#e9d024"
/ApEinfo_revcolor="#e9d024"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
promoter 4541..4948
/note="pLannotate"
/database="snapgene"
/identity="99.5"
/match_length="100.0"
/fragment="False"
/other="promoter"
/locus_tag="LEU2 promoter(2)"
/label="LEU2 promoter(2)"
/ApEinfo_label="LEU2 promoter"
/ApEinfo_fwdcolor="#346ee0"
/ApEinfo_revcolor="#346ee0"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
CDS complement(1..384)
/note="pLannotate"
/database="swissprot"
/identity="69.1"
/match_length="61.0"
/fragment="True"
/other="CDS"
/locus_tag="crp (fragment)"
/label="crp (fragment)"
/ApEinfo_label="crp (fragment)"
/ApEinfo_fwdcolor="#e9d024"
/ApEinfo_revcolor="#e9d024"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ncRNA complement(1667..1771)
/note="pLannotate"
/database="Rfam"
/identity="100.0"
/match_length="102.9"
/fragment="False"
/other="ncRNA"
/locus_tag="RNAI"
/label="RNAI"
/ApEinfo_label="RNAI"
/ApEinfo_fwdcolor="#00D308"
/ApEinfo_revcolor="#00D308"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
CDS complement(4329..4508)
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="14.2"
/fragment="True"
/other="CDS"
/locus_tag="FLP (fragment)"
/label="FLP (fragment)"
/ApEinfo_label="FLP (fragment)"
/ApEinfo_fwdcolor="#e9d024"
/ApEinfo_revcolor="#e9d024"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
protein_bind complement(4147..4176)
/note="pLannotate"
/database="snapgene"
/identity="100.0"
/match_length="62.5"
/fragment="True"
/other="protein_bind"
/locus_tag="FRT (mutant) (fragment)"
/label="FRT (mutant) (fragment)"
/ApEinfo_label="FRT (mutant) (fragment)"
/ApEinfo_fwdcolor="#2D64FE"
/ApEinfo_revcolor="#2D64FE"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
CDS 2919..3008
/note="pLannotate"
/database="swissprot"
/identity="93.3"
/match_length="16.6"
/fragment="True"
/other="CDS"
/locus_tag="RAF1 (fragment)"
/label="RAF1 (fragment)"
/ApEinfo_label="RAF1 (fragment)"
/ApEinfo_fwdcolor="#e9d024"
/ApEinfo_revcolor="#e9d024"
/ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
1 gttctgatcc tcgagcatct taagaattcg tcccacggtt tgtctagagc agccgacaat
61 ctggccaatt tcctgacggg taattttgat ttgcatgccg tccgggtgag tcatagcgtc
121 tggtgacgtc atgcgcatga tatcttcaca ggcggttttc gcacgtaccc atgcgctacg
181 ttcctggccc tcttcaaaca ggcccagttc gccaataaaa tcaccctgat tcagatagga
241 gaggatcatt tctttaccct cttcgtcttt gatcagcact gccacagagc ctttaacgat
301 gtagtacagc gtttccgctt tttcaccctg gtgaataagc gtgctcttgg atgggtactt
361 atgaatgtgg caatgagaca agaacgaaaa gcgtttacct cggaactcta ttgtagaacc
421 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
481 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
541 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
601 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
661 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
721 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
781 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
841 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
901 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
961 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
1021 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
1081 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
1141 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
1201 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
1261 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
1321 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
1381 tcagaccaag tttactcata tatactttag attgatttaa aacttcaaat ccaatcagcg
1441 taaggtgtag actttctctg tcagaccaag tttactcata tatactttag attgatttaa
1501 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
1561 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
1621 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
1681 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
1741 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
1801 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
1861 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
1921 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
1981 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
2041 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
2101 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
2161 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
2221 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
2281 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
2341 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
2401 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
2461 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
2521 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
2581 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
2641 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
2701 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
2761 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
2821 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
2881 accgatgaaa cgagatcgta tctgctgcgt aaatagtagt caacgatcgt acttgttacc
2941 catcattgaa ttttgaacat ccgaacctgg gagttttccc tgaaacagat agtatatttg
3001 aacctgtata ataatatata gtctagcgct ttacggaaga caatgtatgt atttcggttc
3061 ctggagaaac tattgcatct attgcatagg taatcttgca cgtcgcatcc ccggttcatt
3121 ttctgcgttt ccatcttgca cttcaatagc atatctttgt taacgaagca tctgtgcttc
3181 attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
3241 gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg
3301 cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct
3361 gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat
3421 ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc
3481 atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt
3541 tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat
3601 aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt
3661 ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg
3721 tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct
3781 tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat
3841 tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa
3901 cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta
3961 ggttatatag ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg
4021 tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt
4081 tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt
4141 ctagctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct
4201 tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct
4261 gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa
4321 atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat
4381 attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct
4441 atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat
4501 attggatcta aaatctcgta aaggaactgt ctgctctgtt aactgtggga atactcaggt
4561 atcgtaagat gcaagagttc gaatctctta gcaaccatta tttttttcct caacataacg
4621 agaacacaca ggggcgctat cgcacagaat caaattcgat gactggaaat tttttgttaa
4681 tttcagaggt cgcctgacgc atataccttt ttcaactgaa aaattgggag aaaaaggaaa
4741 ggtgagagcc gcggaaccgg cttttcatat agaatagaga agcgttcatg actaaatgct
4801 tgcatcacaa tacttgaagt tgacaatatt atttaaggac ctattgtttt ttccaatagg
4861 tggttagcaa tcgtcttact ttctaacttt tcttaccttt tacatttcag caatatatat
4921 atatatattt caaggatata ccattctaat gtctgcccct aagaagatcg tcgttttgcc
4981 aggtgaccac gttggtcaag aaatcacagc cgaagccatt aaggttctta aagctatttc
5041 tgatgttcgt tccaatgtca agttcgattt cgaaaatcat ttaattggtg gtgctgctat
5101 cgatgctaca ggtgtcccac ttccagatga ggcgctggaa gcctccaaga aggttgatgc
5161 cgttttgtta ggtgctgtgg gtggtcctaa atggggtaca ggtagtgtta gacctgaaca
5221 aggtttacta aaaatccgta aagaacttca attgtacgcc aacttaagac catgtaactt
5281 tgcatccgac tctcttttag acttatctcc aatcaagcca caatttgcta aaggtactga
5341 cttcgttgtt gtcagagaat tagtgggagg tatttacttt ggtaagagaa aggaagacga
5401 tggtgatggt gtcgcttggg atagtgaaca atacaccgtt ccagaagtgc aaagaatcac
5461 aagaatggcc gctttcatgg ccctacaaca tgagccacca ttgcctattt ggtccttgga
5521 taaagctaat gttttggcct cttcaagatt atggagaaaa actgtggagg aaaccatcaa
5581 gaacgaattt cctacattga aggttcaaca tcaattgatt gattctgccg ccatgatcct
5641 agttaagaac ccaacccacc taaatggtat tataatcacc agcaacatgt ttggtgatat
5701 catctccgat gaagcctccg ttatcccagg ttccttgggt ttgttgccat ctgcgtcctt
5761 ggcctctttg ccagacaaga acaccgcatt tggtttgtac gaaccatgcc acggttctgc
5821 tccagatttg ccaaagaata aggtcaaccc tatcgccact atcttgtctg ctgcaatgat
5881 gttgaaattg tcattgaact tgcctgaaga aggtaaggcc attgaagatg cagttaaaaa
5941 ggttttggat gcaggtatca gaactggtga tttaggtggt tccaacagta ccacggaagt
6001 cggtgatgct gtcgccgaag aagttaagaa aatccttgct taaaaagatt ctcttttttt
6061 atgatatttg tacataaact ttataaatga aattcataat agaaacgaca cgaaattaca
6121 aaatggaata tgttcatagg gtagaaactg taaaatcagg tatctcgtag tccgt
//