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Copy pathYIplac204_snapgene.gb
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YIplac204_snapgene.gb
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LOCUS . 3545 bp DNA circular UNK 01-JAN-1980
DEFINITION .
ACCESSION <unknown id>
VERSION <unknown id>
KEYWORDS .
SOURCE
ORGANISM .
.
COMMENT pydna cdseguid=24fcVzVrHWTQXwerHr3_OyEw9D0 2024-06-11T18:43:03
FEATURES Location/Qualifiers
promoter complement(1450..1551)
/gene="S. cerevisiae TRP1"
/label="TRP1 promoter"
/note="color: #ffffff"
CDS complement(775..1449)
/codon_start=1
/gene="S. cerevisiae TRP1"
/note="yeast auxotrophic marker"
/note="color: #ff7f50"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/transl_table=1
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK*"
/label="TRP1"
rep_origin 696..936
/note="S. cerevisiae autonomously replicating sequence
ARS1/ARS416"
/note="color: #ffff00"
/label="ARS1"
protein_bind 180..196
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
/note="color: #31849b"
/label="lac operator"
primer_bind 204..220
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0"
/label="M13 rev"
primer_bind complement(290..306)
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0"
/label="M13 fwd"
promoter 142..172
/note="promoter for the E. coli lac operon"
/note="color: #ffffff"
/label="lac promoter"
misc_feature 233..289
/gene=""
/note="pUC19 multiple cloning site"
/note="color: #99ccff"
/label="MCS"
promoter 1639..1743
/gene="bla"
/label="AmpR promoter"
/note="color: #ffffff"
CDS 216..539
/codon_start=1
/gene="lacZ"
/product="LacZα fragment of β-galactosidase"
/transl_table=1
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
DAA*"
/label="lacZα"
/note="color: #993366"
rep_origin 2775..3363
/direction=RIGHT
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
/label="ori"
CDS 1744..2604
/codon_start=1
/gene="bla"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="color: #ccffcc"
/product="β-lactamase"
/transl_table=1
/translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
SLIKHW*"
/label="AmpR"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
661 caccgtcatc accgaaacgc gcgagacgaa agggcgatct tttatgcttg cttttcaaaa
721 ggcttgcagg caagtgcaca aacaatactt aaataaatac tactcagtaa taacctattt
781 cttagcattt ttgacgaaat ttgctatttt gttagagtct tttacaccat ttgtctccac
841 acctccgctt acatcaacac caataacgcc atttaatcta agcgcatcac caacattttc
901 tggcgtcagt ccaccagcta acataaaatg taagctctcg gggctctctt gccttccaac
961 ccagtcagaa atcgagttcc aatccaaaag ttcacctgtc ccacctgctt ctgaatcaaa
1021 caagggaata aacgaatgag gtttctgtga agctgcactg agtagtatgt tgcagtcttt
1081 tggaaatacg agtcttttaa taactggcaa accgaggaac tcttggtatt cttgccacga
1141 ctcatctcca tgcagttgga cgatatcaat gccgtaatca ttgaccagag ccaaaacatc
1201 ctccttaggt tgattacgaa acacgccaac caagtatttc ggagtgcctg aactattttt
1261 atatgctttt acaagacttg aaattttcct tgcaataacc gggtcaattg ttctctttct
1321 attgggcaca catataatac ccagcaagtc agcatcggaa tctagtgcac attctgcggc
1381 ctctgtgctc tgcaagccgc aaactttcac caatggacca gaactacctg tgaaattaat
1441 aacagacata ctccaagctg cctttgtgtg cttaatcacg tatactcacg tgctcaatag
1501 tcaccaatgc cctccctctt ggccctctcc ttttcttttt tcgaccgaat tggcctcgtg
1561 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
1621 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
1681 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
1741 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
1801 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
1861 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
1921 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
1981 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
2041 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
2101 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
2161 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
2221 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
2281 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
2341 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
2401 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
2461 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
2521 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
2581 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
2641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
2701 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
2761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
2821 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
2881 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
2941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
3001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
3061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
3121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
3181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
3241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
3301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
3361 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
3421 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
3481 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
3541 gaaga
//