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YIplac128_snapgene.gb
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LOCUS Exported 4293 bp DNA circular SYN 12-MAR-2019
DEFINITION Yeast integrating plasmid with a LEU2 marker. See also YIplac204 and
YIplac211.
ACCESSION .
VERSION .
KEYWORDS YIplac128.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 4293)
AUTHORS Gietz RD, Sugino A.
TITLE New yeast-Escherichia coli shuttle vectors constructed with in vitro
mutagenized yeast genes lacking six-base pair restriction sites.
JOURNAL Gene 1988;74:527-34.
PUBMED 3073106
REFERENCE 2 (bases 1 to 4293)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 28, 2022 from SnapGene Viewer 6.0.2
https://www.snapgene.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene";
date: "1988"; volume: "74"; pages: "527-34"
The sequence of this plasmid was reconstructed using the description
from Gietz and Sugino.
pydna cdseguid=Ch6Y7zxAoVN5PAGDHKdzT2AFmm8 2024-06-11T18:43:06
FEATURES Location/Qualifiers
source 1..4293
/lab_host="Saccharomyces cerevisiae"
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 142..172
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 180..196
/label="lac operator"
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 204..220
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 216..539
/codon_start=1
/gene="lacZ"
/product="LacZ-alpha fragment of beta-galactosidase"
/label="lacZ-alpha"
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
DAA"
misc_feature 233..289
/label="MCS"
/note="pUC19 multiple cloning site"
primer_bind complement(290..306)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(797..1891)
/codon_start=1
/gene="S. cerevisiae LEU2"
/product="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/label="LEU2"
/note="yeast auxotrophic marker"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA"
promoter complement(1892..2299)
/gene="S. cerevisiae LEU2"
/label="LEU2 promoter"
promoter 2387..2491
/gene="bla"
/label="AmpR promoter"
CDS 2492..3352
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3523..4111
/direction=RIGHT
/label="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
661 caccgtcatc accgaaacgc gcgagacgaa agggcctacc ctatgaacat attccatttt
721 gtaatttcgt gtcgtttcta ttatgaattt catttataaa gtttatgtac aaatatcata
781 aaaaaagaga atctttttaa gcaaggattt tcttaacttc ttcggcgaca gcatcaccga
841 cttccgtggt actgttggaa ccacctaaat caccagttct gatacctgca tccaaaacct
901 ttttaactgc atcttcaatg gccttacctt cttcaggcaa gttcaatgac aatttcaaca
961 tcattgcagc agacaagata gtggcgatag ggttgacctt attctttggc aaatctggag
1021 cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca
1081 aggacgcaga tggcaacaaa cccaaggaac ctgggataac ggaggcttca tcggagatga
1141 tatcaccaaa catgttgctg gtgattataa taccatttag gtgggttggg ttcttaacta
1201 ggatcatggc ggcagaatca atcaattgat gttgaacctt caatgtagga aattcgttct
1261 tgatggtttc ctccacagtt tttctccata atcttgaaga ggccaaaaca ttagctttat
1321 ccaaggacca aataggcaat ggtggctcat gttgtagggc catgaaagcg gccattcttg
1381 tgattctttg cacttctgga acggtgtatt gttcactatc ccaagcgaca ccatcaccat
1441 cgtcttcctt tctcttacca aagtaaatac ctcccactaa ttctctgaca acaacgaagt
1501 cagtaccttt agcaaattgt ggcttgattg gagataagtc taaaagagag tcggatgcaa
1561 agttacatgg tcttaagttg gcgtacaatt gaagttcttt acggattttt agtaaacctt
1621 gttcaggtct aacactacct gtaccccatt taggaccacc cacagcacct aacaaaacgg
1681 catcaacctt cttggaggct tccagcgcct catctggaag tgggacacct gtagcatcga
1741 tagcagcacc accaattaaa tgattttcga aatcgaactt gacattggaa cgaacatcag
1801 aaatagcttt aagaacctta atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg
1861 gcaaaacgac gatcttctta ggggcagaca ttagaatggt atatccttga aatatatata
1921 tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat tgctaaccac
1981 ctattggaaa aaacaatagg tccttaaata atattgtcaa cttcaagtat tgtgatgcaa
2041 gcatttagtc atgaacgctt ctctattcta tatgaaaagc cggttccgcg gctctcacct
2101 ttcctttttc tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga cctctgaaat
2161 taacaaaaaa tttccagtca tcgaatttga ttctgtgcga tagcgcccct gtgtgttctc
2221 gttatgttga ggaaaaaaat aatggttgct aagagattcg aactcttgca tcttacgata
2281 cctgagtatt cccacagttg gcctcgtgat acgcctattt ttataggtta atgtcatgat
2341 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
2401 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
2461 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
2521 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
2581 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
2641 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
2701 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
2761 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
2821 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
2881 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
2941 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
3001 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
3061 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
3121 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
3181 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
3241 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
3301 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
3361 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
3421 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
3481 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
3541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
3601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
3661 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
3721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
3781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
3841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
3901 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
3961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
4021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
4081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
4141 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
4201 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
4261 gcgcagcgag tcagtgagcg aggaagcgga aga
//