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Copy pathYCplac33_snapgene.gb
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YCplac33_snapgene.gb
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LOCUS . 5603 bp DNA circular UNK 01-JAN-1980
DEFINITION .
ACCESSION <unknown id>
VERSION <unknown id>
KEYWORDS .
SOURCE
ORGANISM .
.
COMMENT pydna cdseguid=XyTO5X31FUQgGt9m4roqPDTDzZs 2024-06-11T18:43:06
FEATURES Location/Qualifiers
CDS 3802..4662
/codon_start=1
/gene="bla"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="color: #ccffcc"
/product="β-lactamase"
/transl_table=1
/translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
SLIKHW*"
/label="AmpR"
rep_origin 4833..5421
/direction=RIGHT
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
/label="ori"
CDS 216..554
/codon_start=1
/gene="lacZ"
/product="LacZα fragment of β-galactosidase"
/transl_table=1
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIYRWAILMKN
ILNLLSYY*"
/label="lacZα"
/note="color: #993366"
promoter 3697..3801
/gene="bla"
/label="AmpR promoter"
/note="color: #ffffff"
misc_feature 233..289
/gene=""
/note="pUC19 multiple cloning site"
/note="color: #99ccff"
/label="MCS"
promoter 142..172
/note="promoter for the E. coli lac operon"
/note="color: #ffffff"
/label="lac promoter"
primer_bind complement(290..306)
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0"
/label="M13 fwd"
primer_bind 204..220
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0"
/label="M13 rev"
protein_bind 180..196
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
/note="color: #31849b"
/label="lac operator"
misc_feature 1064..2227
/note="S. cerevisiae CEN4 centromere fused to the
autonomously replicating sequence ARS1/ARS416"
/note="color: #454f59"
/label="CEN/ARS"
CDS complement(2562..3365)
/codon_start=1
/gene="S. cerevisiae URA3"
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/note="color: #ff7f50"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/transl_table=1
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
/label="URA3"
promoter complement(3366..3586)
/gene="S. cerevisiae URA3"
/label="URA3 promoter"
/note="color: #ffffff"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tatcgctggg ccattctcat gaagaatatc ttgaatttat
541 tgtcatatta ctagttggtg tggaagtcct aatatcggtg atcaatatag tggttgacat
601 gctggctagt caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca
661 aatatcaaac ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa
721 gaaaaaaatt actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg
781 tctcaccata gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata
841 catataatac acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa
901 attggctagt gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg
961 taaaagttca cattagcata catatattac acgagccaga aatagtaact tttgcctaaa
1021 tcacaaattg caaaatttaa ttgcttgcaa aaggtcacat gcttataatc aactttttta
1081 aaaatttaaa atactttttt attttttatt tttaaacata aatgaaataa tttatttatt
1141 gtttatgatt accgaaacat aaaacctgct caagaaaaag aaactgtttt gtccttggaa
1201 aaaaagcact acctaggagc ggccaaaatg ccgaggcttt catagcttaa actctttaca
1261 gaaaataggc attatagatc agttcgagtt ttcttattct tccttccggt tttatcgtca
1321 cagttttaca gtaaataagt atcacctctt agagttcgat gataagctgt caaacatgag
1381 aattaattcc cacatgttaa aatagtgaag gagcatgttc ggcacacagt ggaccgaacg
1441 tggggtaagt gcactagggt ccggttaaac ggatctcgca ttgatgaggc aacgctaatt
1501 atcaacatat agattgttat ctatctgcat gaacacgaaa tctttacttg acgacttgag
1561 gctgatggtg tttatgcaaa gaaaccactg tgtttaatat gtgtcactgt ttgatattac
1621 tgtcagcgta gaagataata gtaaaagcgg ttaataagtg tatttgagat aagtgtgata
1681 aagtttttac agcgaaaaga cgataaatac aagaaaatga ttacgaggat acggagagag
1741 gtatgtacat gtgtatttat atactaagct gccggcggtt gtttgcaaga ccgagaaaag
1801 gctagcaaga atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt
1861 gattccattg cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa
1921 tcaatcaaaa agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta
1981 tgtttagatc ttttatgctt gcttttcaaa aggcttgcag gcaagtgcac aaacaatact
2041 taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt
2101 tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc
2161 catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat
2221 gtaagctctc ggggctctct tgccttccaa cccagtcaga aatcgagttc caatccaaaa
2281 gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga ggtttctgtg
2341 aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta ataactggca
2401 aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg acgatcgatg
2461 ataagctgtc aaacatgaga attgggtaat aactgatata attaaattga agctctaatt
2521 tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg ctggccgcat
2581 cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac cttagcatcc
2641 cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt agagaccaca
2701 tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa acccacaccg
2761 ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct ttgagcaata
2821 aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt agtatattct
2881 ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc tctaggttcc
2941 tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc caccacaccg
3001 tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga gtactgcaat
3061 ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa attgtacttg
3121 gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt caagatatcc
3181 acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc cagtaattcc
3241 ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg catgatatta
3301 aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata tgtagctttc
3361 gacatgattt atcttcgttt cctgcatgtt tttgttctgt gcagttgggt taagaatact
3421 gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct aagtctgtgc
3481 tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt tcaaagaaac
3541 cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga attgaaaagc taattcttga
3601 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
3661 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
3721 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
3781 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
3841 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
3901 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
3961 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
4021 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
4081 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
4141 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
4201 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
4261 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
4321 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
4381 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
4441 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
4501 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
4561 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
4621 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
4681 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
4741 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
4801 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
4861 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
4921 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
4981 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
5041 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
5101 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
5161 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
5221 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
5281 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
5341 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
5401 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
5461 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
5521 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
5581 tcagtgagcg aggaagcgga aga
//