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Copy pathYCplac22_snapgene.gb
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YCplac22_snapgene.gb
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LOCUS . 4854 bp DNA circular UNK 01-JAN-1980
DEFINITION .
ACCESSION <unknown id>
VERSION <unknown id>
KEYWORDS .
SOURCE
ORGANISM .
.
COMMENT pydna cdseguid=BB-iNIXT2u55EuDhEHH0yufrGWg 2024-06-11T18:43:06
FEATURES Location/Qualifiers
CDS 3053..3913
/codon_start=1
/gene="bla"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="color: #ccffcc"
/product="β-lactamase"
/transl_table=1
/translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
SLIKHW*"
/label="AmpR"
rep_origin 4084..4672
/direction=RIGHT
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
/label="ori"
CDS 216..554
/codon_start=1
/gene="lacZ"
/product="LacZα fragment of β-galactosidase"
/transl_table=1
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIYRWAILMKN
ILNLLSYY*"
/label="lacZα"
/note="color: #993366"
promoter 2948..3052
/gene="bla"
/label="AmpR promoter"
/note="color: #ffffff"
misc_feature 233..289
/gene=""
/note="pUC19 multiple cloning site"
/note="color: #99ccff"
/label="MCS"
promoter 142..172
/note="promoter for the E. coli lac operon"
/note="color: #ffffff"
/label="lac promoter"
primer_bind complement(290..306)
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0"
/label="M13 fwd"
primer_bind 204..220
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0"
/label="M13 rev"
protein_bind 180..196
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
/note="color: #31849b"
/label="lac operator"
misc_feature 1064..2227
/note="S. cerevisiae CEN4 centromere fused to the
autonomously replicating sequence ARS1/ARS416"
/note="color: #454f59"
/label="CEN/ARS"
CDS complement(2066..2740)
/codon_start=1
/gene="S. cerevisiae TRP1"
/note="yeast auxotrophic marker"
/note="color: #ff7f50"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/transl_table=1
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK*"
/label="TRP1"
promoter complement(2741..2842)
/gene="S. cerevisiae TRP1"
/label="TRP1 promoter"
/note="color: #ffffff"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tatcgctggg ccattctcat gaagaatatc ttgaatttat
541 tgtcatatta ctagttggtg tggaagtcct aatatcggtg atcaatatag tggttgacat
601 gctggctagt caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca
661 aatatcaaac ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa
721 gaaaaaaatt actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg
781 tctcaccata gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata
841 catataatac acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa
901 attggctagt gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg
961 taaaagttca cattagcata catatattac acgagccaga aatagtaact tttgcctaaa
1021 tcacaaattg caaaatttaa ttgcttgcaa aaggtcacat gcttataatc aactttttta
1081 aaaatttaaa atactttttt attttttatt tttaaacata aatgaaataa tttatttatt
1141 gtttatgatt accgaaacat aaaacctgct caagaaaaag aaactgtttt gtccttggaa
1201 aaaaagcact acctaggagc ggccaaaatg ccgaggcttt catagcttaa actctttaca
1261 gaaaataggc attatagatc agttcgagtt ttcttattct tccttccggt tttatcgtca
1321 cagttttaca gtaaataagt atcacctctt agagttcgat gataagctgt caaacatgag
1381 aattaattcc cacatgttaa aatagtgaag gagcatgttc ggcacacagt ggaccgaacg
1441 tggggtaagt gcactagggt ccggttaaac ggatctcgca ttgatgaggc aacgctaatt
1501 atcaacatat agattgttat ctatctgcat gaacacgaaa tctttacttg acgacttgag
1561 gctgatggtg tttatgcaaa gaaaccactg tgtttaatat gtgtcactgt ttgatattac
1621 tgtcagcgta gaagataata gtaaaagcgg ttaataagtg tatttgagat aagtgtgata
1681 aagtttttac agcgaaaaga cgataaatac aagaaaatga ttacgaggat acggagagag
1741 gtatgtacat gtgtatttat atactaagct gccggcggtt gtttgcaaga ccgagaaaag
1801 gctagcaaga atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt
1861 gattccattg cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa
1921 tcaatcaaaa agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta
1981 tgtttagatc ttttatgctt gcttttcaaa aggcttgcag gcaagtgcac aaacaatact
2041 taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt
2101 tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc
2161 catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat
2221 gtaagctctc ggggctctct tgccttccaa cccagtcaga aatcgagttc caatccaaaa
2281 gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga ggtttctgtg
2341 aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta ataactggca
2401 aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg acgatatcaa
2461 tgccgtaatc attgaccaga gccaaaacat cctccttagg ttgattacga aacacgccaa
2521 ccaagtattt cggagtgcct gaactatttt tatatgcttt tacaagactt gaaattttcc
2581 ttgcaataac cgggtcaatt gttctctttc tattgggcac acatataata cccagcaagt
2641 cagcatcgga atctagtgca cattctgcgg cctctgtgct ctgcaagccg caaactttca
2701 ccaatggacc agaactacct gtgaaattaa taacagacat actccaagct gcctttgtgt
2761 gcttaatcac gtatactcac gtgctcaata gtcaccaatg ccctccctct tggccctctc
2821 cttttctttt ttcgaccgaa ttaattcttg aagacgaaag ggcctcgtga tacgcctatt
2881 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
2941 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
3001 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
3061 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
3121 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
3181 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
3241 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
3301 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
3361 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
3421 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
3481 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
3541 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
3601 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
3661 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
3721 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
3781 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
3841 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
3901 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
3961 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
4021 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
4081 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
4141 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
4201 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
4261 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
4321 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
4381 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
4441 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
4501 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
4561 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
4621 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
4681 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
4741 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
4801 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aaga
//