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Copy pathYCplac111_snapgene.gb
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YCplac111_snapgene.gb
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LOCUS . 6103 bp DNA circular UNK 01-JAN-1980
DEFINITION .
ACCESSION <unknown id>
VERSION <unknown id>
KEYWORDS .
SOURCE
ORGANISM .
.
COMMENT pydna cdseguid=7ytS0ma8bAjM9lEcozGnQDYtse8 2024-06-11T18:43:07
FEATURES Location/Qualifiers
CDS 4302..5162
/codon_start=1
/gene="bla"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="color: #ccffcc"
/product="β-lactamase"
/transl_table=1
/translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
SLIKHW*"
/label="AmpR"
rep_origin 5333..5921
/direction=RIGHT
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
/label="ori"
CDS 216..500
/codon_start=1
/gene="lacZ"
/product="LacZα fragment of β-galactosidase"
/transl_table=1
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRI"
/label="lacZα"
/note="color: #993366"
promoter 4197..4301
/gene="bla"
/label="AmpR promoter"
/note="color: #ffffff"
misc_feature 233..289
/gene=""
/note="pUC19 multiple cloning site"
/note="color: #99ccff"
/label="MCS"
promoter 142..172
/note="promoter for the E. coli lac operon"
/note="color: #ffffff"
/label="lac promoter"
primer_bind complement(290..306)
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0"
/label="M13 fwd"
primer_bind 204..220
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0"
/label="M13 rev"
protein_bind 180..196
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
/note="color: #31849b"
/label="lac operator"
misc_feature 1066..2229
/note="S. cerevisiae CEN4 centromere fused to the
autonomously replicating sequence ARS1/ARS416"
/note="color: #454f59"
/label="CEN/ARS"
CDS complement(2589..3683)
/codon_start=1
/gene="S. cerevisiae LEU2"
/note="yeast auxotrophic marker"
/note="color: #ff7f50"
/product="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/transl_table=1
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA*"
/label="LEU2"
promoter complement(3684..4091)
/gene="S. cerevisiae LEU2"
/label="LEU2 promoter"
/note="color: #ffffff"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tatcgctggg ccattctcat gaagaatatc ttgaatttat
541 tgtcatatta ctagttggtg tggaagtcct aatatcggtg atcaatatag tggttgacat
601 gctggctagt caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca
661 aatatcaaac ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa
721 gaaaaaaatt actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg
781 tctcaccata gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata
841 catataatac acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa
901 attggctagt gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg
961 taaaagttca cattagcata catatattac acgagccaga aatagtaact tttgcctaaa
1021 tcacaaattg caaaatttaa ttgcttgcaa aaggtcacat gcttataatc aactttttta
1081 aaaatttaaa atactttttt attttttatt tttaaacata aatgaaataa tttatttatt
1141 gtttatgatt accgaaacat aaaacctgct caagaaaaag aaactgtttt gtccttggaa
1201 aaaaagcact acctaggagc ggccaaaatg ccgaggcttt catagcttaa actctttaca
1261 gaaaataggc attatagatc agttcgagtt ttcttattct tccttccggt tttatcgtca
1321 cagttttaca gtaaataagt atcacctctt agagttcgat gataagctgt caaacatgag
1381 aattaattcc cacatgttaa aatagtgaag gagcatgttc ggcacacagt ggaccgaacg
1441 tggggtaagt gcactagggt ccggttaaac ggatctcgca ttgatgaggc aacgctaatt
1501 atcaacatat agattgttat ctatctgcat gaacacgaaa tctttacttg acgacttgag
1561 gctgatggtg tttatgcaaa gaaaccactg tgtttaatat gtgtcactgt ttgatattac
1621 tgtcagcgta gaagataata gtaaaagcgg ttaataagtg tatttgagat aagtgtgata
1681 aagtttttac agcgaaaaga cgataaatac aagaaaatga ttacgaggat acggagagag
1741 gtatgtacat gtgtatttat atactaagct gccggcggtt gtttgcaaga ccgagaaaag
1801 gctagcaaga atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt
1861 gattccattg cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa
1921 tcaatcaaaa agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta
1981 tgtttagatc ttttatgctt gcttttcaaa aggcttgcag gcaagtgcac aaacaatact
2041 taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt
2101 tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc
2161 catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat
2221 gtaagctctc ggggctctct tgccttccaa cccagtcaga aatcgagttc caatccaaaa
2281 gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga ggtttctgtg
2341 aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta ataactggca
2401 aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg acgatcgatg
2461 ataagctgtc aaacatgaga attaattcta ccctatgaac atattccatt ttgtaatttc
2521 gtgtcgtttc tattatgaat ttcatttata aagtttatgt acaaatatca taaaaaaaga
2581 gaatcttttt aagcaaggat tttcttaact tcttcggcga cagcatcacc gacttccgtg
2641 gtactgttgg aaccacctaa atcaccagtt ctgatacctg catccaaaac ctttttaact
2701 gcatcttcaa tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca
2761 gcagacaaga tagtggcgat agggttgacc ttattctttg gcaaatctgg agcagaaccg
2821 tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca
2881 gatggcaaca aacccaagga acctgggata acggaggctt catcggagat gatatcacca
2941 aacatgttgc tggtgattat aataccattt aggtgggttg ggttcttaac taggatcatg
3001 gcggcagaat caatcaattg atgttgaacc ttcaatgtag gaaattcgtt cttgatggtt
3061 tcctccacag tttttctcca taatcttgaa gaggccaaaa cattagcttt atccaaggac
3121 caaataggca atggtggctc atgttgtagg gccatgaaag cggccattct tgtgattctt
3181 tgcacttctg gaacggtgta ttgttcacta tcccaagcga caccatcacc atcgtcttcc
3241 tttctcttac caaagtaaat acctcccact aattctctga caacaacgaa gtcagtacct
3301 ttagcaaatt gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat
3361 ggtcttaagt tggcgtacaa ttgaagttct ttacggattt ttagtaaacc ttgttcaggt
3421 ctaacactac ctgtacccca tttaggacca cccacagcac ctaacaaaac ggcatcaacc
3481 ttcttggagg cttccagcgc ctcatctgga agtgggacac ctgtagcatc gatagcagca
3541 ccaccaatta aatgattttc gaaatcgaac ttgacattgg aacgaacatc agaaatagct
3601 ttaagaacct taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg
3661 acgatcttct taggggcaga cattagaatg gtatatcctt gaaatatata tatatatatt
3721 gctgaaatgt aaaaggtaag aaaagttaga aagtaagacg attgctaacc acctattgga
3781 aaaaacaata ggtccttaaa taatattgtc aacttcaagt attgtgatgc aagcatttag
3841 tcatgaacgc ttctctattc tatatgaaaa gccggttccg cggctctcac ctttcctttt
3901 tctcccaatt tttcagttga aaaaggtata tgcgtcaggc gacctctgaa attaacaaaa
3961 aatttccagt catcgaattt gattctgtgc gatagcgccc ctgtgtgttc tcgttatgtt
4021 gaggaaaaaa ataatggttg ctaagagatt cgaactcttg catcttacga tacctgagta
4081 ttcccacagt taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
4141 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
4201 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
4261 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
4321 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
4381 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
4441 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
4501 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
4561 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
4621 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
4681 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
4741 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
4801 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
4861 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
4921 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
4981 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
5041 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
5101 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
5161 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
5221 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
5281 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
5341 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
5401 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
5461 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
5521 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
5581 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
5641 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
5701 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
5761 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
5821 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
5881 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
5941 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
6001 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
6061 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aga
//