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Add 125 mg Tetracycline Hydrochloride (MW = 480.898 g/mol) powder to a 15 mL FALCON tube.
Add 7 mL of Ethanol 99.5 or 96%.
Add water to 10 mL.
Aliquot 1 mL per tube and store at -20C.
For XL1-Blue add 12.5 µg/mL Tetracycline.
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