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Miscelaneous_NGS_Scripts

Various and sundry collection of scripts I've used for NGS analysis

ScriptForStrandedness.sh

Strandededness check for RNA-Seq using ReSQC and Bedops

GTF_Ensembl_Gene_IDextraction_Mmusv104.sh

gene_ID and gene_name extraction from ensembl GTF (Mmus GRCm39 as example) to a tsv conversion table

GTF_Ensembl_PWSextraction_v104.sh

Extraction of the PWS imprinted domain genes on Mmus Chr7 based on genomic coordinates from ensembl GTF (Mmus GRCm39 as example)

SamtoBam.sh

Convert .sam files using samtools sort then index as bin/bash script

md5sum_script.sh and md5sum_script_gunzip.sh

short bash scripts to get md5 checksum. used prior to NCBI/Geo upload

SRX_IdentifierMetadata.sh

script to extract SRXxxxxxxxx IDs from SRA Run Selector Meta data. This was needed for input into nf-core fetchngs rather than synapse IDs.

'PRJEB6698_metadata.sh'

long one cat/grep/awk/tr/sed one-liner to extract metadata from an .xml file into a simple .tsv

References

Bedops

https://bedops.readthedocs.io/en/latest/

RSeQC

http://rseqc.sourceforge.net/#