SelectionTwo_proNR2-LGK1-CombinedTiles

Dataset published in:
Comprehensive Sequence-Flux Mapping of a Levoglucosan Utilization Pathway in E. coli
Justin R. Klesmith, John-Paul Bacik, Ryszard Michalczyk, and Timothy A. Whitehead
http://pubs.acs.org/doi/full/10.1021/acssynbio.5b00131
DOI: 10.1021/acssynbio.5b00131

QuickNormalize
Justin R. Klesmith
Contact: klesmit3@msu.edu, justinklesmith@gmail.com, justinklesmith@evodyn.com
Copyright 2015, Justin R. Klesmith
Version: 1.8, Build: 20150520
License: BSD-3
Credits: Justin R. Klesmith, Caitlin A. Kowalsky, Timothy A. Whitehead
Normalization run parameters:
16:21:54
05/22/2015
Start residue: 0
Normalization type: growth
Doublings: 12.8
Tile Length: 80
Enrich output directory: ./12/data/output-all/
Unselected counts to be significant: 5
Wild-type sequence file: ./WTSeq
Wild-type log2 (Ewt): 0.934903902012
Normalizing the data
Location,Mutation,Normalized_ER,Unselected_Reads,Selected_Reads,RawLog2
0,*,-0.4870,8,None,-3.0
0,A,-0.9347,49,None,-5.61470984412
0,C,NS,3,None,None
0,D,-0.5931,6,1,-3.69466173464
0,E,-0.5758,12,None,-3.58496250072
0,F,-0.5122,9,None,-3.16992500144
0,G,-0.6960,20,None,-4.32192809489
0,H,-0.6423,16,None,-4.0
0,I,-0.1131,80,161,-0.100710450688
0,K,-0.2654,24,20,-1.37273363975
0,L,-0.4174,53,20,-2.51569159359
0,M,0.000,None,None,None
0,N,-0.5122,9,None,-3.16992500144
0,P,-1.1038,85,None,-6.40939093614
0,Q,-0.8326,34,None,-5.08746284125
0,R,-0.2711,26,21,-1.41782152928
0,S,-0.9802,53,2,-5.83761968848
0,T,-0.2325,74,74,-1.10969923391
0,V,-0.2543,35,31,-1.28478594047
0,W,NS,1,1,-1.10969923391
0,Y,NS,1,None,None
1,*,-0.5931,6,1,-3.69466173464
1,A,0.1214,37,352,2.14027901909
1,C,0.0738,5,34,1.65583551245
1,D,NS,3,3,-1.10969923391
1,E,-0.2325,5,5,-1.10969923391
1,F,-0.2975,10,7,-1.62427240675
1,G,0.0780,6,42,1.69765568814
1,H,0.0797,35,248,1.71521405953
1,I,NS,4,13,0.590740484229
1,K,NS,2,109,4.65848509086
1,L,0.0203,90,425,1.12976670078
1,M,0.2738,6,181,3.80518415245
1,N,NS,4,33,1.93469488545
1,P,0.000,None,None,None
1,Q,0.0586,25,153,1.503832419
1,R,0.0067,45,194,0.998360511942
1,S,0.3217,73,3247,4.36536787649
1,T,0.0358,46,241,1.27962814626
1,V,NS,4,20,1.21222886097
1,W,-0.0327,6,20,0.627266360252
1,Y,0.0780,7,49,1.69765568815
2,*,-0.6704,18,None,-4.16992500144
2,A,-0.2074,72,83,-0.904584804008
2,C,-0.1443,45,75,-0.372733639751
2,D,-0.1972,18,22,-0.82019261672
2,E,NS,2,1,-2.10969923392
2,F,0.1581,21,261,2.52588933987
2,G,-0.1328,14,25,-0.273197966195
2,H,-0.0135,49,185,0.806972382485
2,I,0.000,None,None,None
2,K,0.2206,9,179,3.20419154191
2,L,-0.1722,75,106,-0.610597469845
2,M,0.0846,6,44,1.764769884
2,N,0.0914,29,223,1.83321967088
2,P,-0.3742,239,112,-2.20321111983
2,Q,-0.0383,14,45,0.574798940359
2,R,-0.0842,47,113,0.155890876817
2,S,-0.1328,89,159,-0.272549709595
2,T,0.0237,100,483,1.16232395515
2,V,-0.0491,45,135,0.475263266809
2,W,-0.1410,10,17,-0.344164487553
2,Y,0.0074,24,104,1.0057779835
3,*,-0.6423,16,None,-4.0
3,A,0.000,None,None,None
3,C,0.3220,5,223,4.36927257112
3,D,0.0460,13,73,1.37968560683
3,E,-0.0173,19,70,0.771656269582
3,F,NS,2,6,0.4752632668
3,G,NS,4,78,3.17570298495
3,H,0.0485,14,80,1.40487393891
3,I,-0.2139,9,10,-0.957696140469
3,K,0.0172,16,74,1.09975413172
3,L,0.0852,36,265,1.7702243141
3,M,NS,2,13,1.59074048423
3,N,0.0107,7,31,1.03714215442
3,P,0.0695,155,1023,1.61276679056
3,Q,0.1162,18,165,2.08669797889
3,R,0.0072,55,238,1.00375881587
3,S,0.1761,112,1590,2.7177568942
3,T,0.0582,84,513,1.50079835891
3,V,-0.0320,69,231,0.633525350725
3,W,NS,3,7,0.112693187419
3,Y,-0.0289,12,41,0.662890269983
4,*,-0.5122,9,None,-3.16992500144
4,A,-0.2847,124,93,-1.52473673319
4,C,-0.0832,31,75,0.164923146195
4,D,0.0943,13,102,1.86228638992
4,E,-0.4427,6,2,-2.69466173464
4,F,0.0288,8,40,1.21222886097
4,G,-0.3666,39,19,-2.14717393934
4,H,0.2291,83,1763,3.29907809406
4,I,0.0978,47,378,1.89795433863
4,K,-0.0677,6,16,0.305338265368
4,L,-0.0314,83,279,0.639382646568
4,M,NS,3,16,1.30533826537
4,N,0.1880,29,450,2.84610096217
4,P,-0.2291,519,529,-1.08216605016
4,Q,-0.1512,10,16,-0.431627328798
4,R,-0.0556,139,400,0.415215883137
4,S,0.0206,141,667,1.13229236487
4,T,0.000,None,None,None
4,V,-0.1724,34,48,-0.612199574444
4,W,NS,4,None,None
4,Y,-0.0459,16,49,0.505010610203
5,*,-0.4484,37,12,-2.73419009883
5,A,-0.3447,66,36,-1.98416835183
5,C,-0.0318,17,57,0.635727939001
5,D,NS,4,11,0.349732384724
5,E,-0.1211,12,23,-0.171099778577
5,F,-0.0491,11,33,0.475263266808
5,G,-0.2211,15,16,-1.01658982952
5,H,-0.0431,34,106,0.530758379399
5,I,-0.0160,7,26,0.783385562171
5,K,-0.1486,8,13,-0.409259515771
5,L,-0.0310,65,219,0.642720012659
5,M,NS,2,31,2.84449707647
5,N,0.0027,5,21,0.960690093978
5,P,0.0001,133,549,0.935680669589
5,Q,0.1401,29,316,2.33610051913
5,R,-0.0891,60,140,0.112693187425
5,S,0.000,None,None,None
5,T,0.3834,70,5238,5.11581809349
5,V,-0.0375,26,84,0.582178470719
5,W,NS,4,9,0.0602257675274
5,Y,0.0131,18,81,1.06022576753
6,*,-0.7619,26,None,-4.70043971814
6,A,0.0209,124,588,1.13577680054
6,C,-0.0131,56,212,0.810866298595
6,D,-0.0543,41,119,0.427566524778
6,E,0.2007,23,393,2.98512431229
6,F,0.2729,19,569,3.79465809495
6,G,-0.0047,39,156,0.890300766083
6,H,0.0488,73,418,1.40783533929
6,I,-0.1498,44,71,-0.419383733049
6,K,-0.0001,41,169,0.933628197754
6,L,0.0792,120,847,1.70962832981
6,M,-0.0491,39,117,0.475263266809
6,N,0.0830,32,232,1.74828176122
6,P,0.0126,310,1390,1.05504552843
6,Q,0.0478,51,290,1.39778451413
6,R,-0.0138,159,599,0.80383000359
6,S,0.1000,180,1471,1.92102920101
6,T,0.000,None,None,None
6,V,-0.0709,44,115,0.276359198392
6,W,0.0337,6,31,1.25953457575
6,Y,-0.0125,20,76,0.81630018464
7,*,-0.6287,21,3,-3.91705415597
7,A,0.0204,94,444,1.13012778076
7,C,0.0644,54,344,1.56167801862
7,D,-0.0194,30,109,0.751594495254
7,E,-0.0780,10,25,0.212228860975
7,F,0.0594,13,80,1.51178914283
7,G,0.000,None,None,None
7,H,-0.0032,26,105,0.90410656561
7,I,0.1178,11,102,2.10329448942
7,K,0.2371,5,113,3.38855163361
7,L,0.1929,82,1321,2.90016351271
7,M,0.1306,6,61,2.23607560293
7,N,0.1294,13,131,2.22328404949
7,P,0.0654,188,1206,1.57172610631
7,Q,NS,3,20,1.62726636025
7,R,-0.0497,86,257,0.469660560579
7,S,0.0345,103,535,1.26719532019
7,T,0.0717,60,402,1.63446186166
7,V,-0.1086,58,120,-0.0607896334344
7,W,-0.0491,8,24,0.475263266809
7,Y,0.0798,11,78,1.71627136632
8,*,-0.8367,32,2,-5.10969923391
8,A,0.1356,243,2561,2.28797980008
8,C,-0.5163,34,8,-3.19716207517
8,D,0.000,None,None,None
8,E,0.0051,41,175,0.983959873301
8,F,-0.0066,20,79,0.872153419369
8,G,-0.0148,86,322,0.794952889501
8,H,-0.1305,37,67,-0.253063409091
8,I,-0.0003,43,177,0.931641561464
8,K,0.0212,8,38,1.13822827953
8,L,-0.0346,82,270,0.609564358518
8,M,0.1902,9,142,2.87012288415
8,N,0.0389,37,198,1.31020402053
8,P,-0.0254,186,650,0.695437862888
8,Q,-0.0388,39,125,0.570682831886
8,R,-0.3716,139,66,-2.18424618728
8,S,-0.0328,112,373,0.625977664282
8,T,0.0486,99,566,1.40560238884
8,V,-0.0291,85,290,0.660818919963
8,W,-0.2514,10,9,-1.26170232736
8,Y,-0.0698,57,150,0.286229442416
9,*,-0.6850,62,7,-4.25654062225
9,A,0.0545,210,1250,1.46376762797
9,C,0.0288,66,330,1.21222886098
9,D,0.0600,127,785,1.5181649231
9,E,-0.0054,57,227,0.883959239211
9,F,-0.0542,73,212,0.428396661769
9,G,-0.0397,70,223,0.561917649061
9,H,-0.0342,200,660,0.612766790553
9,I,0.0494,190,1092,1.41320229867
9,K,-0.0385,71,228,0.573443660747
9,L,-0.0434,197,613,0.527992210315
9,M,0.3520,25,1432,4.73026035358
9,N,0.000,None,None,None
9,P,-0.1503,434,698,-0.424167240144
9,Q,0.1017,71,587,1.9377703397
9,R,0.0145,276,1254,1.07409794211
9,S,0.0447,295,1642,1.36696803362
9,T,0.0703,271,1799,1.62113119598
9,V,0.1448,103,1161,2.38495249577
9,W,-0.2325,9,9,-1.10969923391
9,Y,-0.0184,128,468,0.760665485669
10,*,-0.8326,34,None,-5.08746284125
10,A,0.0218,224,1068,1.14364177572
10,C,0.0192,35,164,1.11856975376
10,D,0.0150,70,319,1.07843036291
10,E,0.0417,11,60,1.33775974305
10,F,-0.2615,47,40,-1.34235999071
10,G,0.1039,65,546,1.96069009398
10,H,-0.1976,41,50,-0.823395048755
10,I,0.0016,112,467,0.950224583736
10,K,0.1043,19,160,1.96430134753
10,L,-0.4975,183,47,-3.07081022052
10,M,-0.1315,5,9,-0.26170232736
10,N,-0.0771,35,88,0.220449367783
10,P,-0.0327,246,820,0.627266360253
10,Q,0.0408,45,244,1.32918500732
10,R,-0.0509,147,436,0.458812746026
10,S,0.0077,129,560,1.00835652761
10,T,-0.0433,88,274,0.528901230409
10,V,0.000,None,None,None
10,W,-0.4589,7,None,-2.80735492206
10,Y,-0.9464,51,None,-5.67242534197
11,*,-1.1479,45,1,-6.60155233024
11,A,-1.4278,192,2,-7.69466173464
11,C,-0.2272,33,34,-1.06663051203
11,D,-0.9103,45,None,-5.49185309633
11,E,-1.2039,53,1,-6.83761968848
11,F,-0.8908,42,None,-5.39231742278
11,G,-0.8701,39,None,-5.28540221886
11,H,-1.4468,217,None,-7.76155123244
11,I,-0.4923,38,10,-3.03569865247
11,K,-0.9632,54,None,-5.75488750216
11,L,0.000,None,None,None
11,M,-0.1822,15,20,-0.694661734635
11,N,-1.0636,75,None,-6.2288186905
11,P,-0.7819,820,63,-4.81189940992
11,Q,-0.6060,146,23,-3.77596183674
11,R,-0.8417,277,17,-5.13597855872
11,S,-1.1020,235,6,-6.40125367977
11,T,-1.2925,339,5,-7.19291260217
11,V,-0.6397,44,6,-3.98416835184
11,W,-0.3905,5,None,-2.32192809489
11,Y,-0.9632,54,None,-5.75488750216
12,*,-0.8451,33,2,-5.15409335328
12,A,-0.1713,197,280,-0.602468036427
12,C,-0.2241,41,43,-1.04098648383
12,D,0.000,None,None,None
12,E,-0.1061,20,42,-0.0393099060242
12,F,-0.7463,34,3,-4.61219957445
12,G,-0.3454,92,50,-1.9894050002
12,H,-0.4905,147,39,-3.02396935989
12,I,-0.9896,59,None,-5.88264304936
12,K,-0.3721,19,9,-2.18770174592
12,L,-1.3743,252,3,-7.50201665669
12,M,-0.4870,8,None,-3.0
12,N,-0.0477,38,115,0.487863303584
12,P,-1.7170,913,5,-8.62224218901
12,Q,-0.0770,33,83,0.220946078074
12,R,-0.4057,185,74,-2.4316273288
12,S,-0.1008,159,345,0.00787036246777
12,T,-0.8655,160,9,-5.26170232736
12,V,-0.3566,78,40,-2.07317335789
12,W,-0.7415,24,None,-4.58496250072
12,Y,-0.1811,76,102,-0.685201405382
13,*,-0.7308,23,None,-4.52356195606
13,A,-1.5904,140,1,-8.23898225086
13,C,-0.3369,44,25,-1.92527466278
13,D,-1.1334,93,None,-6.53915881111
13,E,-0.3905,5,None,-2.32192809489
13,F,0.000,None,None,None
13,G,-1.0922,38,1,-6.35762674736
13,H,-1.1905,102,2,-6.78212457589
13,I,-0.3944,26,11,-2.35070733342
13,K,-0.4870,8,None,-3.0
13,L,-0.0413,143,451,0.547413052563
13,M,NS,1,2,-0.109699233914
13,N,-0.7992,30,None,-4.90689059561
13,P,-1.6885,1034,6,-8.53875720351
13,Q,-1.1625,47,1,-6.6642880856
13,R,-1.1892,254,5,-6.7764558258
13,S,-0.5312,182,40,-3.29556577923
13,T,-1.3071,141,2,-7.24925058632
13,V,-0.1976,41,50,-0.823395048755
13,W,-0.6271,15,None,-3.90689059561
13,Y,-0.3525,21,11,-2.04258503806
14,*,-0.8840,19,1,-5.35762674736
14,A,-0.1841,157,207,-0.710833025306
14,C,-0.0507,32,95,0.460156374421
14,D,-0.7903,29,None,-4.85798099513
14,E,-0.7415,24,None,-4.58496250072
14,F,-0.6287,14,2,-3.91705415597
14,G,-1.2357,58,1,-6.96768022904
14,H,-0.1033,51,109,-0.0139402511079
14,I,0.0325,24,123,1.2478527707
14,K,0.0299,29,146,1.22214432983
14,L,0.1446,81,912,2.38334077737
14,M,-0.0211,27,97,0.735326106111
14,N,-0.1168,31,61,-0.133158206738
14,P,-0.9473,806,34,-5.67687242119
14,Q,-0.0780,32,80,0.212228860976
14,R,0.1834,105,1574,2.79627507393
14,S,-0.1391,157,270,-0.327504385754
14,T,0.000,None,None,None
14,V,0.0413,34,185,1.33421938535
14,W,-0.4272,6,None,-2.58496250072
14,Y,-0.5853,29,5,-3.64575213416
15,*,-0.9996,61,None,-5.93073733756
15,A,-0.5354,487,105,-3.32323167833
15,C,-0.5834,92,16,-3.63326118997
15,D,-0.6568,134,17,-4.08832558312
15,E,-1.0507,72,None,-6.16992500144
15,F,-0.5359,93,20,-3.32692995014
15,G,-0.7000,226,24,-4.34491569561
15,H,-1.3529,318,4,-7.4225821892
15,I,0.0098,57,251,1.02895430587
15,K,-0.8908,42,None,-5.39231742278
15,L,-0.1338,311,552,-0.281945547271
15,M,-0.7387,11,1,-4.56913085255
15,N,-1.1903,110,None,-6.78135971352
15,P,-1.4774,1515,14,-7.86744639021
15,Q,-1.0704,142,4,-6.25944635342
15,R,-1.4836,549,5,-7.88843347803
15,S,-1.2680,444,7,-7.09676017821
15,T,-0.3957,371,156,-2.35957239168
15,V,0.000,None,None,None
15,W,-0.8079,31,None,-4.95419631039
15,Y,-1.4958,113,1,-7.92987819633
16,*,-0.6960,20,None,-4.32192809489
16,A,-1.3305,75,1,-7.33851792441
16,C,-0.4705,24,7,-2.88730681258
16,D,-0.6423,16,None,-4.0
16,E,-0.4589,7,None,-2.80735492206
16,F,-0.0047,8,32,0.890300766087
16,G,-0.7308,23,None,-4.52356195606
16,H,-1.0237,66,None,-6.04439411936
16,I,-0.0279,16,55,0.671660479611
16,K,-0.6835,19,None,-4.24792751344
16,L,0.000,None,None,None
16,M,-0.1211,12,23,-0.171099778577
16,N,-0.8163,32,None,-5.0
16,P,-0.6007,428,69,-3.74264176353
16,Q,-0.4057,50,20,-2.4316273288
16,R,-0.8905,175,9,-5.3909853443
16,S,-1.2266,122,None,-6.93073733756
16,T,-0.9768,131,5,-5.82119414057
16,V,-0.1190,17,33,-0.152767955805
16,W,-0.5122,9,None,-3.16992500144
16,Y,-0.7619,26,None,-4.70043971814
17,*,NS,4,None,None
17,A,-0.8873,173,9,-5.37440246011
17,C,-0.1822,48,64,-0.694661734633
17,D,-0.2856,67,50,-1.5319322346
17,E,-0.5563,11,None,-3.45943161864
17,F,-0.5758,12,None,-3.58496250072
17,G,0.000,None,None,None
17,H,-1.0464,71,None,-6.1497471195
17,I,-1.2169,55,1,-6.89105894744
17,K,-0.4589,7,None,-2.80735492206
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P,-1.1038,0.000,-0.3742,0.0695,-0.2291,0.0001,0.0126,0.0654,-0.0254,-0.1503,-0.0327,-0.7819,-1.7170,-1.6885,-0.9473,-1.4774,-0.6007,-2.0297,-0.5698,-1.6356,-1.2418,-1.1625,-0.6549,-2.3475,-1.2117,-1.5694,-1.6191,-1.2744,-1.4453,-1.3254,-1.1272,-0.9455,-0.9951,-1.3289,-1.6681,-1.5857,-1.2203,0.0460,-0.3696,0.000,-0.6908,-0.7488,0.000,-1.3827,-1.5217,-1.7909,-1.5904,-0.5838,-0.8939,-1.6153,-1.6663,-1.1409,-1.0164,-1.2314,0.000,-0.5992,-0.0191,-0.3324,0.000,-1.3097,-1.3391,-0.9838,-1.0461,-1.4594,-1.3708,-0.9366,-1.2126,-1.3283,-0.7875,-1.4547,-1.1577,-0.8339,-0.7870,-1.0389,0.000,-0.4602,-1.1169,-0.5322,-0.6423,-1.3480,
Q,-0.8326,0.0586,-0.0383,0.1162,-0.1512,0.1401,0.0478,NS,-0.0388,0.1017,0.0408,-0.6060,-0.0770,-1.1625,-0.0780,-1.0704,-0.4057,-0.5563,-0.2811,-0.6960,-0.3905,-0.8326,-0.3905,-0.7811,-0.8840,-0.9406,-0.8766,-1.3790,-0.8701,-0.6942,-0.9359,-0.7478,-1.3305,-0.0577,-1.0567,0.0215,0.000,0.0316,-0.0327,-0.1213,-0.0581,-0.0780,-0.2187,-1.0507,-0.0466,-0.8701,-0.1836,-0.6672,-0.2044,-0.0756,-1.5144,-0.1578,-0.2194,-0.1068,-0.3488,-0.5234,0.1585,0.000,-0.0125,-0.4589,-0.4106,0.000,-0.4073,-0.7308,-0.8481,-0.2145,-1.1768,-0.8974,-0.2586,-0.0425,-0.2780,NS,-1.1553,-0.5205,-0.1693,-0.5563,-0.4350,-0.3699,-0.9256,-0.0083,
R,-0.2711,0.0067,-0.0842,0.0072,-0.0556,-0.0891,-0.0138,-0.0497,-0.3716,0.0145,-0.0509,-0.8417,-0.4057,-1.1892,0.1834,-1.4836,-0.8905,-0.7976,-0.9406,-1.1768,-0.1858,-0.6202,-0.3412,-0.3167,-0.7836,-1.5739,-1.0401,-1.6673,-1.3553,-0.6184,-1.6799,-1.1054,-0.7702,-0.1166,-1.1802,0.1119,-0.4802,0.1103,-0.0855,-0.2861,-0.1033,-0.0541,-0.4859,-0.7148,-0.7308,-1.1661,-0.9687,-0.8612,-0.1491,-0.6459,-1.3545,-0.6643,-0.8956,-0.1012,-0.5822,-0.8865,0.0476,0.0636,-0.1348,-0.6835,-0.3259,0.0614,0.000,-1.1935,-0.8840,0.000,-0.8532,-1.6033,-0.4028,-0.1605,-0.3165,0.1188,-0.7816,-0.0733,-0.7294,-0.7680,-0.7811,-0.2601,-1.1516,-0.8478,
S,-0.9802,0.3217,-0.1328,0.1761,0.0206,0.000,0.1000,0.0345,-0.0328,0.0447,0.0077,-1.1020,-0.1008,-0.5312,-0.1391,-1.2680,-1.2266,-0.4166,-1.1905,-0.3333,0.000,-0.3008,-0.1351,0.000,-1.3995,-1.2243,0.0392,-0.9557,-1.6033,-0.5244,-0.3108,-1.5361,-0.7794,-0.0791,-0.7106,-0.0432,-1.8178,0.0996,-0.0137,-0.2367,0.0188,0.0362,-0.2488,-0.9509,-0.5931,-0.4077,-0.1009,-1.5431,-0.9569,-0.0890,-0.1444,-0.0517,-0.1886,-0.2694,-0.2455,-1.2061,-0.1122,-0.0998,-0.1077,-0.5445,-1.4527,0.0145,-0.7470,-1.6847,-0.8282,-0.4396,-1.4674,-0.5623,-0.6045,-0.1811,-0.4187,0.2489,0.0661,0.000,-0.2984,0.000,-1.2655,-1.0842,0.000,-0.6343,
T,-0.2325,0.0358,0.0237,0.0582,0.000,0.3834,0.000,0.0717,0.0486,0.0703,-0.0433,-1.2925,-0.8655,-1.3071,0.000,-0.3957,-0.9768,-1.3504,-1.1479,-0.6397,-0.4270,-0.9576,0.000,-0.8532,-0.5311,-1.6674,-0.5181,-0.6277,-1.2178,-0.4765,-0.5586,-1.8345,-0.8863,-0.2002,-1.9661,-0.0287,-1.5030,0.0674,0.000,-0.0274,0.0361,-0.0159,-0.1443,-0.4496,-0.1797,-1.1169,0.0233,-1.3813,-0.7505,-0.3013,-0.8003,-0.2017,-0.1469,0.0161,-0.3454,-1.2736,-0.3302,-0.1084,0.0337,-0.2229,-0.6690,-0.1462,-0.7250,-1.0409,-0.3851,-0.3857,-0.6664,-0.4616,-0.4213,0.0949,-0.3599,0.000,0.000,-0.2224,-0.0878,-0.3692,-1.0748,-1.4061,-0.3495,NS,
V,-0.2543,NS,-0.0491,-0.0320,-0.1724,-0.0375,-0.0709,-0.1086,-0.0291,0.1448,0.000,-0.6397,-0.3566,-0.1976,0.0413,0.000,-0.1190,-0.0780,-0.2469,-0.5563,-0.5352,-0.2937,-0.5352,-0.9686,-0.3311,-0.3432,-0.5537,-0.2911,-0.2153,-0.0686,-0.3519,-0.4954,-1.0388,-1.1715,-0.4996,-0.0322,-1.0464,0.0512,-0.0845,-0.0891,-0.0500,-0.0882,-0.1822,0.0699,-0.0652,-0.6354,-0.2028,-0.4427,-0.0160,-0.3463,-1.1939,-0.1387,-0.1625,0.000,-0.3919,-0.4229,-0.2341,0.0279,NS,-0.0193,-0.7196,-0.5288,-0.7287,0.000,-0.2769,-0.8701,-0.5310,-0.2200,-0.3538,-0.3919,-0.4528,0.0932,-1.0074,-0.6960,NS,-0.8404,-0.1621,-0.1797,-0.7308,-0.3729,
W,NS,-0.0327,-0.1410,NS,NS,NS,0.0337,-0.0491,-0.2514,-0.2325,-0.4589,-0.3905,-0.7415,-0.6271,-0.4272,-0.8079,-0.5122,NS,NS,NS,NS,-0.3905,NS,-0.6704,-0.6960,-0.6704,-0.7717,-0.5563,NS,-0.3177,-1.0377,-1.0279,-0.7537,-0.2986,NS,-0.2380,-0.7308,-0.1866,NS,NS,-0.0407,NS,-0.4272,-0.8163,NS,-0.4272,NS,-0.6271,-0.5120,-0.9792,-0.4012,-0.1738,-0.5120,-0.0891,NS,-0.3265,-0.3017,-0.2937,NS,NS,NS,NS,-0.4870,NS,-0.5931,-0.7816,-0.8326,-0.6287,-0.7915,-0.4310,-0.4272,NS,-0.5122,NS,NS,NS,NS,-0.4870,-0.5563,NS,
Y,NS,0.0780,0.0074,-0.0289,-0.0459,0.0131,-0.0125,0.0798,-0.0698,-0.0184,-0.9464,-0.9632,-0.1811,-0.3525,-0.5853,-1.4958,-0.7619,-0.7308,-0.6567,-0.1653,-0.7619,-0.8690,-0.3905,-0.9103,-0.8840,-0.4691,-0.9190,-1.2936,-0.3654,-0.3460,-1.6065,-0.6702,-0.4883,-0.3093,-0.3706,0.000,-0.9745,-0.0605,-0.2234,NS,0.0324,-0.0411,-0.4057,-0.9288,-0.2847,-0.3827,-0.6567,-0.6835,-0.1398,-0.4741,0.000,-0.0173,-1.1697,-0.0865,-0.4870,-0.4249,-0.2737,-0.3802,NS,-0.3905,-0.7903,-0.4589,-0.8840,-0.7196,-0.0969,-0.7415,-1.3972,-0.7735,-0.0446,-0.7662,-0.3309,-0.1713,-0.7196,-0.6423,NS,-0.3008,-0.4870,-0.3905,-0.7619,-0.7308,

QuickNormalize
Justin R. Klesmith
Contact: klesmit3@msu.edu, justinklesmith@gmail.com, justinklesmith@evodyn.com
Copyright 2015, Justin R. Klesmith
Version: 1.8, Build: 20150520
License: BSD-3
Credits: Justin R. Klesmith, Caitlin A. Kowalsky, Timothy A. Whitehead
Normalization run parameters:
16:21:54
05/22/2015
Start residue: 80
Normalization type: growth
Doublings: 12.6
Tile Length: 80
Enrich output directory: ./34/data/output-all/
Unselected counts to be significant: 5
Wild-type sequence file: ./WTSeq
Wild-type log2 (Ewt): 1.39434824608
Normalizing the data
Location,Mutation,Normalized_ER,Unselected_Reads,Selected_Reads,RawLog2
80,*,NS,1,None,None
80,A,-0.2277,39,67,-0.649035863933
80,C,-0.2638,5,7,-0.94429600836
80,D,-0.2103,20,38,-0.503723416977
80,E,NS,3,None,None
80,F,-0.2582,9,13,-0.899208118839
80,G,-0.0820,14,58,0.620903237536
80,H,-0.8147,25,None,-4.64385618977
80,I,-0.0731,13,57,0.70272746049
80,K,-0.8599,11,1,-4.88915445417
80,L,-0.2916,5,6,-1.1666884297
80,M,NS,1,None,None
80,N,-0.5151,7,None,-2.80735492206
80,P,-1.2406,38,1,-6.67765034898
80,Q,-0.4453,5,None,-2.32192809489
80,R,-0.8447,28,None,-4.80735492206
80,S,-0.7931,23,None,-4.52356195606
80,T,-0.3566,26,22,-1.67073093504
80,V,0.000,None,None,None
80,W,NS,None,None,None
80,Y,NS,1,None,None
81,*,-0.5437,8,None,-3.0
81,A,-0.8139,111,12,-4.63917620116
81,C,-0.7172,17,None,-4.08746284125
81,D,-0.2551,51,75,-0.873329487008
81,E,NS,3,None,None
81,F,-0.7816,22,None,-4.45943161864
81,G,-1.1708,31,1,-6.38391914592
81,H,-0.6380,95,21,-3.60726102108
81,I,-0.4215,61,37,-2.15100680747
81,K,-0.3252,29,29,-1.42972283553
81,L,-1.5452,83,1,-7.80476226687
81,M,-0.4827,6,None,-2.58496250072
81,N,0.000,None,None,None
81,P,-1.2475,155,4,-6.70584724081
81,Q,-0.8067,9,1,-4.59964783697
81,R,-1.6510,105,1,-8.1439683532
81,S,-0.4260,86,51,-2.18356224827
81,T,-0.8022,115,13,-4.57477316834
81,V,-0.9128,40,3,-5.1666884297
81,W,NS,3,None,None
81,Y,-0.3753,56,43,-1.81081300289
82,*,-0.4453,5,None,-2.32192809489
82,A,-0.1284,30,93,0.202545379969
82,C,NS,4,None,None
82,D,NS,2,None,None
82,E,NS,3,41,2.34286666836
82,F,-0.4453,5,None,-2.32192809489
82,G,-0.0172,16,101,1.22848864722
82,H,NS,4,None,None
82,I,NS,1,None,None
82,K,NS,2,None,None
82,L,-0.3415,24,22,-1.55525371762
82,M,-0.0330,16,91,1.07807180467
82,N,NS,4,None,None
82,P,-0.8721,31,None,-4.95419631039
82,Q,-0.5151,7,None,-2.80735492206
82,R,-0.6869,15,None,-3.90689059561
82,S,-0.6530,13,None,-3.70043971814
82,T,-0.7024,16,None,-4.0
82,V,0.000,None,None,None
82,W,NS,1,None,None
82,Y,NS,2,None,None
83,*,-0.8250,26,None,-4.70043971814
83,A,-0.6550,73,15,-3.7126567988
83,C,-0.4276,17,10,-2.19525758189
83,D,-1.0741,23,1,-5.95328479159
83,E,NS,2,7,0.377632086523
83,F,-0.1587,45,116,-0.0635949367376
83,G,-0.7172,17,None,-4.08746284125
83,H,-1.0410,62,3,-5.7989566452
83,I,0.000,None,None,None
83,K,-0.7600,30,4,-4.33661343114
83,L,-0.0120,168,1098,1.2786220807
83,M,-0.3252,13,13,-1.42972283554
83,N,-0.3779,33,25,-1.83026076512
83,P,-1.7595,394,3,-8.46681215427
83,Q,-0.0011,34,239,1.38368113119
83,R,-0.2867,73,90,-1.12769429808
83,S,-0.2023,111,221,-0.436236142492
83,T,-0.2116,140,264,-0.514611733118
83,V,-0.1338,45,135,0.155239665183
83,W,-0.4827,6,None,-2.58496250072
83,Y,-0.7816,22,None,-4.45943161864
84,*,-0.4453,5,None,-2.32192809489
84,A,-0.6705,14,None,-3.80735492206
84,C,NS,1,None,None
84,D,-0.4827,6,None,-2.58496250072
84,E,NS,1,None,None
84,F,NS,4,2,-2.42972283553
84,G,NS,4,None,None
84,H,-0.8147,25,None,-4.64385618977
84,I,-0.5929,10,None,-3.32192809489
84,K,-0.6145,11,None,-3.45943161864
84,L,0.000,None,None,None
84,M,-0.0726,5,22,0.707780688221
84,N,NS,4,None,None
84,P,-0.4273,146,86,-2.19328263972
84,Q,-0.2402,15,24,-0.751650930421
84,R,-0.4951,26,11,-2.67073093504
84,S,-0.7446,19,None,-4.24792751344
84,T,-0.7816,22,None,-4.45943161864
84,V,-0.2771,10,13,-1.05121121228
84,W,NS,2,None,None
84,Y,-0.5151,7,None,-2.80735492206
85,*,-0.5695,9,None,-3.16992500144
85,A,-0.8086,136,15,-4.61029508117
85,C,-0.2712,67,90,-1.00395892966
85,D,-0.2749,57,75,-1.03379415921
85,E,-0.6869,15,None,-3.90689059561
85,F,-0.8721,31,None,-4.95419631039
85,G,0.000,None,None,None
85,H,-1.3328,49,1,-7.04443267965
85,I,-0.8147,25,None,-4.64385618977
85,K,NS,None,None,None
85,L,-1.3789,149,None,-7.21916852046
85,M,-0.4827,6,None,-2.58496250072
85,N,-0.7172,17,None,-4.08746284125
85,P,-1.5881,366,4,-7.94542267382
85,Q,-1.1486,29,1,-6.28770383066
85,R,-1.0375,286,14,-5.78223925026
85,S,-0.3949,98,68,-1.9569698384
85,T,-1.2428,103,None,-6.68650052718
85,V,-0.3008,85,97,-1.23920092948
85,W,NS,None,None,None
85,Y,-0.7446,19,None,-4.24792751344
86,*,-0.4784,72,33,-2.55525371761
86,A,-0.6344,196,44,-3.58500106101
86,C,-0.6571,54,11,-3.72517871906
86,D,-0.2814,78,99,-1.08576843431
86,E,0.000,None,None,None
86,F,-0.6524,77,16,-3.69650937623
86,G,-0.3507,71,62,-1.62527364465
86,H,-0.3066,104,115,-1.28467250273
86,I,-0.2748,60,79,-1.03283268297
86,K,-0.3820,58,43,-1.86143907596
86,L,-0.4409,158,87,-2.29056008787
86,M,-0.2582,9,13,-0.899208118839
86,N,-0.2997,54,62,-1.23041402731
86,P,-1.7856,553,4,-8.54085850577
86,Q,-0.2941,49,58,-1.18645168452
86,R,-1.2475,155,4,-6.70584724081
86,S,-0.7982,209,24,-4.55211946689
86,T,-0.6357,206,46,-3.59266140666
86,V,-0.2317,103,173,-0.68159513508
86,W,-0.9647,16,1,-5.42972283553
86,Y,-0.4733,66,31,-2.51992064451
87,*,-0.6344,12,None,-3.58496250072
87,A,-0.6720,136,26,-3.81674595864
87,C,-0.2916,10,12,-1.1666884297
87,D,-0.9057,39,3,-5.13016255367
87,E,NS,1,3,0.155239665186
87,F,-0.9461,30,2,-5.33661343115
87,G,-0.8041,24,None,-4.58496250072
87,H,0.000,None,None,None
87,I,-0.6344,12,None,-3.58496250072
87,K,NS,4,5,-1.10779474064
87,L,-0.5079,201,80,-2.75884643183
87,M,-0.3252,6,6,-1.42972283554
87,N,-0.2638,20,28,-0.944296008363
87,P,-1.2759,1091,26,-6.82071850372
87,Q,-0.2906,63,76,-1.15907524559
87,R,-0.3680,227,181,-1.75642543574
87,S,-0.3186,111,115,-1.37864865094
87,T,-0.1047,115,413,0.41478508494
87,V,-0.5621,42,13,-3.12160054018
87,W,-0.4453,5,None,-2.32192809489
87,Y,-0.2829,54,68,-1.09714749644
88,*,-0.6303,35,8,-3.55900585249
88,A,-1.6284,100,1,-8.07357902531
88,C,-0.5961,64,17,-3.34225999429
88,D,-1.0025,49,None,-5.61470984412
88,E,-0.7172,17,None,-4.08746284125
88,F,0.000,None,None,None
88,G,-1.0155,57,3,-5.67765034898
88,H,-0.7544,44,6,-4.30419195345
88,I,-0.5459,48,16,-3.01468533625
88,K,-0.7312,18,None,-4.16992500144
88,L,-0.3591,170,142,-1.68936665217
88,M,NS,3,None,None
88,N,-1.0692,61,None,-5.93073733756
88,P,-2.1293,502,2,-9.40126638949
88,Q,-0.9432,40,None,-5.32192809489
88,R,-1.8138,146,1,-8.61954739441
88,S,-0.5259,205,75,-2.88038424454
88,T,-1.6899,114,1,-8.26261284969
88,V,-0.4577,73,37,-2.41009402878
88,W,-0.5151,7,None,-2.80735492206
88,Y,-0.3920,44,31,-1.93495814378
89,*,-1.0741,23,1,-5.95328479159
89,A,0.000,None,None,None
89,C,-1.1252,54,2,-6.1846103377
89,D,-1.1606,81,None,-6.33985000288
89,E,-0.5766,69,20,-3.21631919743
89,F,-1.2146,95,None,-6.56985560833
89,G,-0.7049,66,11,-4.01468533625
89,H,-1.2847,86,2,-6.85598759024
89,I,-1.2120,70,2,-6.55900585248
89,K,-0.8975,34,None,-5.08746284125
89,L,-1.7045,235,2,-8.3062397821
89,M,-0.7931,23,None,-4.52356195606
89,N,-1.0025,49,None,-5.61470984412
89,P,-0.9884,746,43,-5.54648990109
89,Q,-1.1128,70,None,-6.12928301694
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QuickNormalize
Justin R. Klesmith
Contact: klesmit3@msu.edu, justinklesmith@gmail.com, justinklesmith@evodyn.com
Copyright 2015, Justin R. Klesmith
Version: 1.8, Build: 20150520
License: BSD-3
Credits: Justin R. Klesmith, Caitlin A. Kowalsky, Timothy A. Whitehead
Normalization run parameters:
16:21:54
05/22/2015
Start residue: 160
Normalization type: growth
Doublings: 12.9
Tile Length: 80
Enrich output directory: ./56/data/output-all/
Unselected counts to be significant: 5
Wild-type sequence file: ./WTSeq
Wild-type log2 (Ewt): 1.13012432196
Normalizing the data
Location,Mutation,Normalized_ER,Unselected_Reads,Selected_Reads,RawLog2
160,*,-1.3514,128,1,-7.40125135025
160,A,-1.0423,68,None,-6.08746284125
160,C,-0.3881,288,84,-2.17885892891
160,D,-1.1941,109,None,-6.76818432478
160,E,-0.9021,42,None,-5.39231742278
160,F,-0.8286,32,None,-5.0
160,G,-0.6102,99,10,-3.70867987545
160,H,-0.3395,327,123,-1.81188367041
160,I,-1.2633,133,None,-7.0552824355
160,K,-0.8524,35,None,-5.12928301694
160,L,-0.5880,305,34,-3.56645394145
160,M,-1.0511,70,None,-6.12928301694
160,N,-0.9213,45,None,-5.49185309633
160,P,-1.0992,680,11,-6.35121066775
160,Q,-1.1517,96,None,-6.58496250072
160,R,0.000,None,None,None
160,S,-0.7497,374,21,-4.55582838736
160,T,-1.6658,270,1,-8.47806694731
160,V,-1.4105,197,None,-7.62205181946
160,W,-0.6709,17,None,-4.08746284125
160,Y,-0.9086,43,None,-5.4262647547
161,*,-0.4593,255,52,-2.69516506897
161,A,-1.5609,283,None,-8.14465824283
161,C,-1.0737,57,1,-6.23414136442
161,D,-1.0848,78,None,-6.28540221886
161,E,-0.4632,40,8,-2.72317944513
161,F,-0.9396,48,None,-5.58496250072
161,G,-1.1121,85,None,-6.40939093614
161,H,-0.3634,187,62,-1.99394949975
161,I,-1.0239,64,None,-6.0
161,K,-0.5233,60,9,-3.13821694442
161,L,-0.6648,276,22,-4.05034418839
161,M,-0.6089,13,None,-3.70043971814
161,N,-1.1685,101,None,-6.65821148275
161,P,-1.1353,968,14,-6.51275966548
161,Q,0.000,None,None,None
161,R,-0.4228,270,66,-2.43367282795
161,S,-1.6228,325,None,-8.34429590792
161,T,-1.6282,249,1,-8.36125328232
161,V,-1.2064,113,None,-6.82017896242
161,W,-0.6089,13,None,-3.70043971814
161,Y,-0.9275,46,None,-5.52356195606
162,*,-0.7098,20,None,-4.32192809489
162,A,-0.5457,111,15,-3.288776621
162,C,-0.1982,47,39,-0.670437983067
162,D,-0.1274,48,61,-0.0554765134098
162,E,-0.6845,18,None,-4.16992500144
162,F,-0.5030,8,None,-3.0
162,G,0.000,None,None,None
162,H,-0.5507,10,None,-3.32192809489
162,I,-0.7659,19,1,-4.6491788637
162,K,NS,2,None,None
162,L,-0.8029,29,None,-4.85798099513
162,M,NS,1,None,None
162,N,-0.4752,7,None,-2.80735492206
162,P,-0.8674,37,None,-5.20945336563
162,Q,-0.5716,11,None,-3.45943161864
162,R,-0.6671,114,9,-4.06421636298
162,S,-0.2279,53,37,-0.919718439192
162,T,-0.7749,26,None,-4.70043971814
162,V,-0.3377,79,30,-1.79814150282
162,W,-0.5909,12,None,-3.58496250072
162,Y,-0.5280,9,None,-3.16992500144
163,*,-1.2154,116,None,-6.85798099513
163,A,0.000,None,None,None
163,C,-1.0725,75,None,-6.2288186905
163,D,-1.0511,70,None,-6.12928301694
163,E,-0.5312,83,12,-3.19132828088
163,F,-0.8117,30,None,-4.90689059561
163,G,-0.6102,99,10,-3.70867987545
163,H,-1.2552,130,None,-7.02236781303
163,I,-1.1879,107,None,-6.7414669864
163,K,-0.9021,42,None,-5.39231742278
163,L,-1.3859,185,None,-7.53138146052
163,M,-0.7650,25,None,-4.64385618977
163,N,-1.1447,94,None,-6.55458885168
163,P,-0.8762,463,16,-5.25611973351
163,Q,-1.0888,79,None,-6.30378074818
163,R,-1.5374,203,1,-8.06658726744
163,S,-0.5360,283,40,-3.2239814982
163,T,-0.4997,316,53,-2.97711164387
163,V,-0.4162,190,48,-2.38614445786
163,W,-0.6567,16,None,-4.0
163,Y,-0.9455,49,None,-5.61470984412
164,*,NS,None,None,None
164,A,-0.5248,47,7,-3.14848527987
164,C,-0.4438,6,None,-2.58496250072
164,D,NS,None,None,None
164,E,NS,4,None,None
164,F,NS,None,None,None
164,G,-0.4752,7,None,-2.80735492206
164,H,-0.7441,23,None,-4.52356195606
164,I,NS,1,None,None
164,K,-0.5030,8,None,-3.0
164,L,-0.1920,129,111,-0.618062739324
164,M,NS,None,None,None
164,N,NS,None,None,None
164,P,0.000,None,None,None
164,Q,-0.2749,43,23,-1.3039541489
164,R,-0.6732,52,4,-4.10169106839
164,S,-0.2746,84,45,-1.3017156767
164,T,-0.3690,59,19,-2.03596688616
164,V,-0.4752,7,None,-2.80735492206
164,W,NS,2,None,None
164,Y,NS,1,None,None
165,*,-0.9513,50,None,-5.64385618977
165,A,-1.0598,72,None,-6.16992500144
165,C,-0.6845,18,None,-4.16992500144
165,D,-0.4752,7,None,-2.80735492206
165,E,-0.5030,8,None,-3.0
165,F,-0.5909,12,None,-3.58496250072
165,G,-0.6567,16,None,-4.0
165,H,-0.8746,38,None,-5.24792751344
165,I,-0.6258,14,None,-3.80735492206
165,K,-0.5030,8,None,-3.0
165,L,0.000,None,None,None
165,M,-0.1974,12,10,-0.66428575609
165,N,-0.5909,12,None,-3.58496250072
165,P,-0.4177,311,78,-2.39661990152
165,Q,-0.3260,47,19,-1.70791268848
165,R,-0.4025,96,26,-2.28577413283
165,S,-1.0377,67,None,-6.06608919046
165,T,-0.9945,58,None,-5.85798099513
165,V,-0.6445,23,2,-3.92481330631
165,W,-0.4075,5,None,-2.32192809489
165,Y,-0.6567,16,None,-4.0
166,*,-1.4842,358,2,-7.88506712752
166,A,-1.5002,186,1,-7.94041016136
166,C,-0.7789,100,5,-4.72317944514
166,D,-1.2154,116,None,-6.85798099513
166,E,-0.9841,56,None,-5.80735492206
166,F,-0.2580,148,87,-1.16776122003
166,G,-1.1083,84,None,-6.39231742278
166,H,-0.5602,174,22,-3.38476322747
166,I,0.000,None,None,None
166,K,-1.2470,127,None,-6.98868468677
166,L,-1.1620,300,4,-6.63007004075
166,M,-0.7075,45,3,-4.30814194586
166,N,-0.4676,189,37,-2.75404040884
166,P,-1.4569,335,2,-7.7892686356
166,Q,-1.1815,105,None,-6.71424551767
166,R,-1.5202,195,1,-8.008581664
166,S,-0.5217,397,60,-3.12735595179
166,T,-0.4805,348,64,-2.8441948461
166,V,-0.1381,132,157,-0.151024720716
166,W,-0.6974,19,None,-4.24792751344
166,Y,-1.2579,131,None,-7.03342300154
167,*,-1.7209,400,None,-8.64385618977
167,A,0.000,None,None,None
167,C,-1.2791,139,None,-7.11894107272
167,D,-1.3399,164,None,-7.35755200462
167,E,-0.7091,151,10,-4.31772799469
167,F,-1.4557,167,1,-7.78495564273
167,G,-0.1806,151,139,-0.520715016855
167,H,-1.5701,289,None,-8.1749256825
167,I,-1.5725,440,2,-8.18261106378
167,K,-1.0807,77,None,-6.26678654069
167,L,-2.1057,798,None,-9.64024493622
167,M,-1.1961,83,1,-6.7762907816
167,N,-1.3165,154,None,-7.26678654069
167,P,-0.8198,820,35,-4.95144843281
167,Q,-1.4602,223,None,-7.80089989992
167,R,-1.7160,396,None,-8.62935662008
167,S,-0.5286,574,84,-3.17384085415
167,T,-0.5153,655,102,-3.08417710471
167,V,-0.5747,397,47,-3.47965769572
167,W,-0.9945,58,None,-5.85798099513
167,Y,-1.5275,262,None,-8.03342300154
168,*,-1.0467,69,None,-6.10852445678
168,A,-0.9021,42,None,-5.39231742278
168,C,-0.3669,43,14,-2.0201611829
168,D,-0.6089,13,None,-3.70043971814
168,E,-0.4752,7,None,-2.80735492206
168,F,0.000,None,None,None
168,G,-0.7098,20,None,-4.32192809489
168,H,-1.1531,73,1,-6.59107590914
168,I,-0.2449,60,38,-1.06021443242
168,K,NS,4,None,None
168,L,-0.3387,217,82,-1.80525057808
168,M,-0.5030,8,None,-3.0
168,N,-0.7098,20,None,-4.32192809489
168,P,-1.5092,190,1,-7.97110695859
168,Q,-1.0045,60,None,-5.90689059561
168,R,-1.0928,80,None,-6.32192809489
168,S,-0.4223,200,49,-2.43039769591
168,T,-0.9598,78,2,-5.68665356911
168,V,-0.3727,38,12,-2.06421636297
168,W,-0.5716,11,None,-3.45943161864
168,Y,-0.3499,59,21,-1.89157697683
169,*,-0.5286,41,6,-3.17384085415
169,A,-1.3353,162,None,-7.33985000288
169,C,-0.3752,77,24,-2.08307539022
169,D,-0.9086,43,None,-5.4262647547
169,E,-0.4752,7,None,-2.80735492206
169,F,0.000,None,None,None
169,G,-0.8954,41,None,-5.35755200462
169,H,-1.2660,134,None,-7.06608919046
169,I,-0.5553,116,15,-3.35234174977
169,K,-0.9275,46,None,-5.52356195606
169,L,-0.2348,237,159,-0.977111643865
169,M,-0.4075,5,None,-2.32192809489
169,N,-0.9841,56,None,-5.80735492206
169,P,-1.8152,365,1,-8.91300400402
169,Q,-0.7845,27,None,-4.75488750216
169,R,-1.1551,97,None,-6.59991284219
169,S,-0.4567,305,63,-2.6766368592
169,T,-1.5664,217,1,-8.1628025827
169,V,-0.6981,101,7,-4.25210791095
169,W,-0.5507,10,None,-3.32192809489
169,Y,-0.3511,82,29,-1.90082235974
170,*,-1.1083,84,None,-6.39231742278
170,A,-0.3382,169,64,-1.80213078654
170,C,-0.9455,49,None,-5.61470984412
170,D,0.000,None,None,None
170,E,-0.1578,36,38,-0.323248838252
170,F,-1.0598,72,None,-6.16992500144
170,G,-0.2871,80,40,-1.40125135025
170,H,-0.5314,90,13,-3.19266472844
170,I,-1.1341,91,None,-6.5077946402
170,K,-0.9336,47,None,-5.55458885168
170,L,-1.4606,169,1,-7.80213078654
170,M,-0.4752,7,None,-2.80735492206
170,N,-0.1998,73,60,-0.684185313525
170,P,-1.6300,250,1,-8.36703563492
170,Q,-0.9213,45,None,-5.49185309633
170,R,-1.5059,249,None,-7.96000193207
170,S,-1.4639,225,None,-7.81378119122
170,T,-1.2884,108,1,-7.15613885242
170,V,-0.1571,67,71,-0.317593421203
170,W,-0.4438,6,None,-2.58496250072
170,Y,-0.2373,62,41,-0.997895656017
171,*,-1.1941,109,None,-6.76818432478
171,A,0.000,None,None,None
171,C,-0.4978,65,11,-2.96418754464
171,D,-0.4605,74,15,-2.70381412027
171,E,-0.7749,26,None,-4.70043971814
171,F,-0.9086,43,None,-5.4262647547
171,G,-0.6384,56,5,-3.88667817743
171,H,-0.7441,23,None,-4.52356195606
171,I,-1.1517,96,None,-6.58496250072
171,K,-0.8817,39,None,-5.28540221886
171,L,-1.4772,176,1,-7.86068296889
171,M,-0.8600,36,None,-5.16992500144
171,N,-0.8524,35,None,-5.12928301694
171,P,-1.2384,563,6,-6.95327996161
171,Q,-0.5716,11,None,-3.45943161864
171,R,-1.1196,87,None,-6.44294349585
171,S,-0.1358,213,257,-0.130336421285
171,T,-0.3152,196,84,-1.62364377159
171,V,-0.2526,66,40,-1.12371737473
171,W,-0.4438,6,None,-2.58496250072
171,Y,-0.9513,50,None,-5.64385618977
172,*,-1.6300,250,1,-8.36703563492
172,A,-1.6710,273,1,-8.49400849117
172,C,-0.8817,39,None,-5.28540221886
172,D,-1.0888,79,None,-6.30378074818
172,E,-1.0641,73,None,-6.18982455888
172,F,-1.0045,60,None,-5.90689059561
172,G,-1.3092,151,None,-7.23840473933
172,H,-1.2731,207,2,-7.09473830775
172,I,-1.0511,70,None,-6.12928301694
172,K,-0.9789,55,None,-5.78135971352
172,L,0.000,None,None,None
172,M,-0.2602,31,18,-1.1855226592
172,N,-0.9150,44,None,-5.45943161864
172,P,-0.6737,1042,80,-4.10446281764
172,Q,-0.4704,145,28,-2.77380551821
172,R,-1.0764,345,6,-6.2467414012
172,S,-1.5970,307,None,-8.26209484537
172,T,-1.5609,283,None,-8.14465824283
172,V,-0.6981,101,7,-4.25210791095
172,W,-0.7441,23,None,-4.52356195606
172,Y,-0.9625,52,None,-5.70043971814
173,*,-1.4547,220,None,-7.78135971352
173,A,-1.8293,375,1,-8.95199813564
173,C,-1.2242,119,None,-6.89481776331
173,D,-1.2277,91,1,-6.90904599046
173,E,-1.0423,68,None,-6.08746284125
173,F,-0.9789,55,None,-5.78135971352
173,G,-1.3445,166,None,-7.37503943135
173,H,-1.4510,218,None,-7.76818432478
173,I,-1.1910,108,None,-6.75488750216
173,K,-1.0045,60,None,-5.90689059561
173,L,0.000,None,None,None
173,M,-0.2817,33,17,-1.35818262836
173,N,-0.9893,57,None,-5.83289001416
173,P,-0.6854,1423,104,-4.17553157864
173,Q,-0.5159,206,32,-3.08775187744
173,R,-0.6936,538,38,-4.22478619936
173,S,-1.8930,422,1,-9.12235053896
173,T,-1.6367,335,None,-8.38801728535
173,V,-0.7477,159,9,-4.5442093041
173,W,-0.8117,30,None,-4.90689059561
173,Y,-0.9945,58,None,-5.85798099513
174,*,-1.3284,159,None,-7.31288295528
174,A,-1.7774,448,None,-8.80735492206
174,C,-1.2003,111,None,-6.79441586635
174,D,-1.0210,48,1,-5.98621385097
174,E,-0.8524,35,None,-5.12928301694
174,F,-1.3689,177,None,-7.46760555008
174,G,-1.2579,131,None,-7.03342300154
174,H,-1.9200,443,1,-9.19241423881
174,I,-1.3688,134,1,-7.46734054071
174,K,-1.2686,135,None,-7.07681559705
174,L,0.000,None,None,None
174,M,-0.3942,46,13,-2.22437358817
174,N,-1.3829,139,1,-7.52019242298
174,P,-0.6514,1374,116,-3.96743664395
174,Q,-0.7098,318,21,-4.32181688276
174,R,-1.0996,619,10,-6.35311885458
174,S,-1.9142,579,None,-9.17741953799
174,T,-1.8480,513,None,-9.00281501561
174,V,-0.7775,179,9,-4.71514212608
174,W,-0.7547,24,None,-4.58496250072
174,Y,-1.3284,159,None,-7.31288295528
175,*,-1.4888,239,None,-7.90086680798
175,A,-1.3963,190,None,-7.56985560833
175,C,-1.1447,94,None,-6.55458885168
175,D,-0.7135,123,8,-4.34376585559
175,E,-0.9995,59,None,-5.88264304936
175,F,-1.3667,176,None,-7.45943161864
175,G,-1.0569,54,1,-6.15613885242
175,H,0.000,None,None,None
175,I,-1.5437,206,1,-8.08775187744
175,K,-1.0767,76,None,-6.24792751344
175,L,-0.5075,495,80,-3.03060797033
175,M,-1.1298,68,1,-6.4887141915
175,N,-0.5548,108,14,-3.34878393036
175,P,-0.7771,715,36,-4.71312578047
175,Q,-0.2758,109,58,-1.3114546799
175,R,-0.2706,199,109,-1.26969164602
175,S,-1.5452,273,None,-8.09275714092
175,T,-1.5389,269,None,-8.07146236256
175,V,-1.2470,127,None,-6.98868468677
175,W,-0.5507,10,None,-3.32192809489
175,Y,-0.3280,160,64,-1.72317944514
176,*,-1.0272,98,2,-6.01596119437
176,A,-1.8745,408,1,-9.07367669223
176,C,-1.1121,85,None,-6.40939093614
176,D,-0.5307,69,10,-3.18784771215
176,E,-0.8746,38,None,-5.24792751344
176,F,-1.3130,231,2,-7.25300039167
176,G,-1.3146,116,1,-7.25923234538
176,H,0.000,None,None,None
176,I,-1.4282,206,None,-7.68650052718
176,K,-0.9081,65,2,-5.42361916328
176,L,-0.6224,679,65,-3.78615130147
176,M,-0.8367,33,None,-5.04439411936
176,N,-0.4971,159,27,-2.95924680337
176,P,-0.9036,1215,38,-5.4000644353
176,Q,-0.4394,200,45,-2.5532544437
176,R,-0.4739,374,71,-2.79839869063
176,S,-1.6228,325,None,-8.34429590792
176,T,-1.8438,509,None,-8.99152184608
176,V,-1.4675,227,None,-7.82654848729
176,W,-0.5716,11,None,-3.45943161864
176,Y,-0.4943,337,58,-2.93987513631
177,*,-0.8117,30,None,-4.90689059561
177,A,-0.7485,195,11,-4.54915004536
177,C,-0.5716,11,None,-3.45943161864
177,D,-0.7547,24,None,-4.58496250072
177,E,-0.2930,31,15,-1.44855706503
177,F,-1.4301,207,None,-7.6934869575
177,G,-1.1664,76,1,-6.6491788637
177,H,-1.2277,91,1,-6.90904599046
177,I,-0.9995,59,None,-5.88264304936
177,K,-0.7232,16,1,-4.40125135025
177,L,-0.4672,474,93,-2.75083578804
177,M,-0.8203,31,None,-4.95419631039
177,N,-0.8447,34,None,-5.08746284125
177,P,0.000,None,None,None
177,Q,-0.4569,189,39,-2.67809155561
177,R,-0.7649,246,13,-4.64332613745
177,S,-0.4175,247,62,-2.39542227145
177,T,-0.6220,219,21,-3.78372098708
177,V,-1.1233,88,None,-6.45943161864
177,W,-0.7650,25,None,-4.64385618977
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Q,-1.1517,0.000,-0.5716,-1.0888,-0.2749,-0.3260,-1.1815,-1.4602,-1.0045,-0.7845,-0.9213,-0.5716,-0.4704,-0.5159,-0.7098,-0.2758,-0.4394,-0.4569,0.0026,-0.4029,-0.2702,-0.9513,-1.1941,-0.9447,0.000,-0.8447,-1.0807,-0.5338,NS,NS,-0.6416,-1.0725,-0.6416,-0.4075,-0.8817,-0.7749,-0.1467,-0.2693,-0.7547,NS,-0.1574,-0.8817,-0.0298,0.0079,-0.5507,-0.5949,-0.1314,-1.8251,-0.9778,-1.3353,-1.5479,-0.8746,-0.4398,-1.3213,-0.1640,-0.9735,-1.2213,-1.0239,-1.4565,-1.3038,-1.1551,-1.0791,-0.7606,-0.7441,-0.9625,-0.2246,-0.8814,-0.5507,-0.0515,-0.2571,-0.0187,0.000,-0.3280,-1.2277,-1.1517,-0.0982,-0.8905,-1.5008,0.0232,-1.1664,
R,0.000,-0.4228,-0.6671,-1.5374,-0.6732,-0.4025,-1.5202,-1.7160,-1.0928,-1.1551,-1.5059,-1.1196,-1.0764,-0.6936,-1.0996,-0.2706,-0.4739,-0.7649,-0.2633,-0.1238,-0.8754,0.000,-1.8797,-0.3062,-0.3949,-1.1341,-1.6189,-0.1994,-0.2111,-0.8746,-0.9569,-1.2944,-0.6709,-0.2225,-1.2552,-0.9513,-0.4224,-0.4875,-0.8817,0.0573,-0.2849,-0.7474,0.000,0.000,-0.8954,-1.4204,-1.4074,-1.4533,-1.5085,-1.4065,-0.8708,-0.4942,-1.1290,-1.0001,-0.6469,-0.7959,-2.0656,-1.4295,-2.2629,-1.6662,-1.3009,-1.5583,-1.2365,-0.8524,0.000,-0.3324,-1.3114,-1.0641,-0.1192,-0.6597,-0.0298,0.0385,-1.2200,-1.1653,-1.6057,-0.0357,-0.9984,-0.8038,-0.0562,-0.1113,
S,-0.7497,-1.6228,-0.2279,-0.5360,-0.2746,-1.0377,-0.5217,-0.5286,-0.4223,-0.4567,-1.4639,-0.1358,-1.5970,-1.8930,-1.9142,-1.5452,-1.6228,-0.4175,-0.0577,-0.1154,-1.2500,-0.5260,-0.6634,-0.4411,-1.2686,-0.2754,-0.8720,-0.0432,-0.1196,-0.5275,-0.2517,-0.5512,-0.7217,-0.1351,-0.4148,-0.6032,-0.3211,-0.3870,-0.9447,-0.2274,-0.2166,-0.2748,-0.0139,-0.4864,0.0305,-0.3821,-0.1988,-0.9557,-1.0493,-1.2121,-0.6636,-0.9920,-0.4296,-0.9047,-0.6683,-0.4702,-0.4548,-1.1649,-0.6624,-1.3130,-1.3688,-0.4983,-1.5762,-1.2277,-0.4647,-0.9491,-0.5197,0.0009,-0.0341,-0.2544,-0.1183,-0.0790,-1.1857,-0.7374,-1.8123,-0.6429,-0.6254,-0.5847,-0.0014,-0.9172,
T,-1.6658,-1.6282,-0.7749,-0.4997,-0.3690,-0.9945,-0.4805,-0.5153,-0.9598,-1.5664,-1.2884,-0.3152,-1.5609,-1.6367,-1.8480,-1.5389,-1.8438,-0.6220,0.000,-0.6815,-0.5852,-1.0848,-0.5416,-1.2358,-0.9369,-0.5606,-0.5075,-0.7098,-0.6845,-0.3068,-0.0676,-0.6866,-0.6567,-0.0950,-1.3330,-0.2083,-0.1224,-0.3594,-0.9893,-0.1330,-1.0748,-1.6066,-0.1432,-0.2204,0.000,-0.9671,-0.9145,-1.3406,-2.2620,-1.2192,-1.8495,-1.2001,0.000,-1.4911,-0.3971,-1.1878,-0.7275,-1.0511,-2.0787,-0.7365,-1.3167,-0.3597,-0.5932,-0.9789,-1.2944,-0.9928,-1.3092,0.000,-0.1873,-1.2064,0.0323,-0.2116,-1.3688,-1.1027,-1.1772,-0.3008,-1.2696,-1.1757,-0.0492,-0.5617,
V,-1.4105,-1.2064,-0.3377,-0.4162,-0.4752,-0.6445,-0.1381,-0.5747,-0.3727,-0.6981,-0.1571,-0.2526,-0.6981,-0.7477,-0.7775,-1.2470,-1.4675,-1.1233,-0.3101,-1.3513,-0.4070,-0.6089,-0.2231,-1.0737,-0.8286,-1.1045,-0.0461,-0.1367,-0.1860,-0.1101,-0.1926,-1.2919,0.000,-0.5909,-0.6544,-0.0975,-0.5030,-0.6974,-0.1389,0.000,-0.3373,-0.5960,-0.1113,-0.1293,-0.4438,-0.2141,-0.1589,-1.4657,-1.4620,-0.5246,-0.7444,-0.2816,-0.3559,-0.2352,-0.9598,-0.5170,-0.4820,0.000,-0.4676,-0.3705,-0.4908,-0.4113,0.000,0.000,-1.0423,-0.2619,-0.5672,-0.6709,-0.5716,-0.3666,-0.0764,-0.0162,-0.2397,-0.7914,-0.3531,-0.1552,-0.2996,-0.7381,0.0087,-0.3251,
W,-0.6709,-0.6089,-0.5909,-0.6567,NS,-0.4075,-0.6974,-0.9945,-0.5716,-0.5507,-0.4438,-0.4438,-0.7441,-0.8117,-0.7547,-0.5507,-0.5716,-0.7650,NS,-0.7217,-0.6089,-0.4752,-0.8149,-0.4012,-0.6416,-0.5716,-0.7217,-0.6089,NS,NS,NS,-0.7938,NS,-0.0881,NS,NS,-0.4075,-0.5507,NS,NS,-0.7749,-0.8369,-0.6258,-0.5010,NS,-0.8203,-0.5280,-0.9455,-0.7098,-0.7075,-1.1551,-0.7650,-0.8600,-0.9150,-0.8524,-0.6416,-0.6416,-0.6416,0.0727,-0.8954,-0.9207,-0.6709,-0.8473,NS,NS,-0.2062,-0.6671,NS,NS,-0.4056,-0.5124,-0.0705,-0.6258,-0.5909,-0.6089,-0.9124,NS,-0.8817,-0.1301,0.0018,
Y,-0.9086,-0.9275,-0.5280,-0.9455,NS,-0.6567,-1.2579,-1.5275,-0.3499,-0.3511,-0.2373,-0.9513,-0.9625,-0.9945,-1.3284,-0.3280,-0.4943,-0.9336,-0.1196,-0.2193,-0.7845,-0.9735,-1.2442,-0.2211,-0.8886,-0.1637,-1.1718,-0.6845,NS,-0.6258,-0.8746,-0.4454,-0.4438,-0.1714,-0.2030,-0.6845,-0.4752,-0.5716,-0.0890,NS,-0.5245,-1.0773,-0.3255,-0.7852,-0.6974,-0.3241,-0.3946,0.000,0.000,-0.7429,-0.4413,-0.4695,-1.5002,-1.6043,-0.9513,-1.2390,-0.3428,-0.8600,-0.2368,-1.6245,-0.5635,-0.9635,-0.9523,-0.7845,-0.8674,-0.0205,0.1742,-0.8286,-0.0797,-0.4528,-0.2590,-0.0411,-0.9396,0.000,-0.3740,-0.6909,-0.4690,-1.3177,-0.0729,-0.8720,

QuickNormalize
Justin R. Klesmith
Contact: klesmit3@msu.edu, justinklesmith@gmail.com, justinklesmith@evodyn.com
Copyright 2015, Justin R. Klesmith
Version: 1.8, Build: 20150520
License: BSD-3
Credits: Justin R. Klesmith, Caitlin A. Kowalsky, Timothy A. Whitehead
Normalization run parameters:
16:21:55
05/22/2015
Start residue: 240
Normalization type: growth
Doublings: 12.3
Tile Length: 80
Enrich output directory: ./78/data/output-all/
Unselected counts to be significant: 5
Wild-type sequence file: ./WTSeq
Wild-type log2 (Ewt): 1.25081449709
Normalizing the data
Location,Mutation,Normalized_ER,Unselected_Reads,Selected_Reads,RawLog2
240,*,-0.7771,21,None,-4.39231742278
240,A,-0.1238,309,641,0.136550570109
240,C,-0.2448,81,84,-0.863699528711
240,D,-0.2446,105,109,-0.862228141496
240,E,-0.8388,28,2,-4.72352187066
240,F,-1.1154,35,1,-6.04544996556
240,G,0.000,None,None,None
240,H,-1.2222,90,None,-6.49185309633
240,I,-0.8834,31,None,-4.95419631039
240,K,-0.5697,9,None,-3.16992500144
240,L,-1.1956,177,4,-6.38377249869
240,M,-0.5135,7,None,-2.80735492206
240,N,-0.9499,39,None,-5.28540221886
240,P,-2.8921,754,1,-10.4745876619
240,Q,-1.0960,33,1,-5.96056106797
240,R,-0.7322,365,39,-4.14251738351
240,S,-0.4203,263,110,-2.17372622438
240,T,-1.9550,237,1,-8.80491019751
240,V,-0.3897,144,70,-1.9568089331
240,W,-0.7509,19,None,-4.24792751344
240,Y,-0.8741,30,None,-4.90689059561
241,*,-0.4442,110,41,-2.33997465751
241,A,0.0360,448,2551,1.59332531294
241,C,-0.2988,80,62,-1.28389873311
241,D,-0.9373,99,5,-5.2235954738
241,E,-0.1643,41,67,-0.207629762759
241,F,-0.7886,93,8,-4.45532575972
241,G,-0.2105,153,192,-0.588592290577
241,H,-0.4313,116,46,-2.25058598768
241,I,-0.3107,169,123,-1.37453187955
241,K,0.000,None,None,None
241,L,-0.1654,303,492,-0.216826426739
241,M,0.0164,32,160,1.40576114628
241,N,-0.2514,85,85,-0.916166948609
241,P,-1.3473,1266,19,-6.97430112459
241,Q,-0.0011,118,526,1.24010899133
241,R,-0.0501,359,1170,0.78828583204
241,S,-0.0005,339,1517,1.24569695851
241,T,-0.6319,324,52,-3.55557723335
241,V,-0.5533,142,32,-3.06591406811
241,W,-1.1248,36,1,-6.08609195005
241,Y,-0.6808,61,8,-3.84690428617
242,*,-1.0197,26,1,-5.61660666675
242,A,-0.2188,253,303,-0.655986539825
242,C,-0.1444,73,134,-0.0399023170318
242,D,-1.0728,58,None,-5.85798099513
242,E,NS,2,None,None
242,F,-0.9499,39,None,-5.28540221886
242,G,-0.7127,104,12,-4.03164416602
242,H,-0.4129,256,111,-2.12175108225
242,I,-0.7315,28,3,-4.13855936994
242,K,-0.2184,5,6,-0.653132542773
242,L,-0.2251,295,341,-0.707110163831
242,M,NS,None,None,None
242,N,-0.2731,27,24,-1.08609195005
242,P,-1.3480,1068,16,-6.97686288029
242,Q,-0.3065,43,32,-1.34243170331
242,R,0.000,None,None,None
242,S,-0.5151,198,53,-2.81760311412
242,T,-0.6673,202,28,-3.7670235093
242,V,-0.4863,36,11,-2.62666033141
242,W,NS,4,None,None
242,Y,-0.8237,25,None,-4.64385618977
243,*,-0.5135,7,None,-2.80735492206
243,A,-0.7504,211,21,-4.24494871453
243,C,-0.1492,65,116,-0.0805537665025
243,D,-0.1888,43,61,-0.41169436575
243,E,-0.5939,10,None,-3.32192809489
243,F,-0.7370,18,None,-4.16992500144
243,G,0.000,None,None,None
243,H,-0.7893,22,None,-4.45943161864
243,I,-0.7072,16,None,-4.0
243,K,-0.6911,15,None,-3.90689059561
243,L,-1.0960,33,1,-5.96056106797
243,M,NS,None,None,None
243,N,-0.4801,6,None,-2.58496250072
243,P,-1.1763,79,None,-6.30378074818
243,Q,-0.6368,12,None,-3.58496250072
243,R,-0.6860,109,14,-3.87699635132
243,S,-0.3201,153,106,-1.44563433674
243,T,-1.1152,66,None,-6.04439411936
243,V,-0.6806,579,76,-3.84565897315
243,W,NS,2,None,None
243,Y,-0.8548,28,None,-4.80735492206
244,*,-0.5426,165,39,-2.99708694399
244,A,-0.1432,245,453,-0.0294376475624
244,C,-0.1371,60,115,0.0224325067365
244,D,-0.2232,95,111,-0.691606690583
244,E,-0.1184,98,210,0.183368724943
244,F,-0.1668,59,95,-0.22895438964
244,G,-1.0197,130,5,-5.61660666675
244,H,-0.0920,146,367,0.413644745358
244,I,0.0012,118,534,1.26188593372
244,K,0.000,None,None,None
244,L,0.0467,172,1052,1.69648728599
244,M,-0.1265,24,49,0.113580394788
244,N,0.0109,102,492,1.35392221476
244,P,-2.0624,846,3,-9.055718301
244,Q,0.0589,84,558,1.81563694045
244,R,0.0359,238,1354,1.59202731166
244,S,-0.0442,180,609,0.842278372969
244,T,-0.0541,212,674,0.752517378013
244,V,0.2086,80,1549,3.3590263853
244,W,NS,4,6,-0.331204447877
244,Y,-0.1233,75,156,0.140416579758
245,*,-0.6560,13,None,-3.70043971814
245,A,-0.5027,173,49,-2.73608533213
245,C,-1.3069,60,1,-6.82305754422
245,D,-0.4459,92,34,-2.35226606341
245,E,-0.6162,11,None,-3.45943161864
245,F,-0.3216,48,33,-1.45673532997
245,G,-0.8248,133,10,-4.64952128922
245,H,-1.0256,50,None,-5.64385618977
245,I,-0.0683,44,128,0.624401432754
245,K,-0.7643,20,None,-4.32192809489
245,L,-0.7618,168,16,-4.30848437138
245,M,NS,1,1,-0.916166948612
245,N,-0.9649,41,None,-5.35755200462
245,P,-0.5763,162,33,-3.21162283213
245,Q,-0.6560,13,None,-3.70043971814
245,R,-1.1763,79,None,-6.30378074818
245,S,-1.3038,119,2,-6.81098471191
245,T,-1.1893,82,None,-6.35755200462
245,V,0.000,None,None,None
245,W,-0.4493,11,4,-2.37559856724
245,Y,-0.8548,28,None,-4.80735492206
246,*,-1.6281,128,1,-7.91616694861
246,A,-0.5876,330,64,-3.28248916285
246,C,-0.0293,46,171,0.978123610219
246,D,0.000,None,None,None
246,E,-0.4042,137,62,-2.06000272118
246,F,-0.9095,72,4,-5.08609195005
246,G,-0.5450,171,40,-3.0120913686
246,H,-0.2237,103,120,-0.695776880177
246,I,-1.4261,153,None,-7.25738784269
246,K,-1.2776,111,2,-6.71058281495
246,L,-1.3231,313,5,-6.88425770065
246,M,-0.9547,42,2,-5.30848437139
246,N,-0.1663,104,168,-0.224289243969
246,P,-2.4605,485,1,-9.83800788569
246,Q,-1.2101,87,None,-6.44294349585
246,R,-2.1856,339,1,-9.32130841175
246,S,-0.3117,192,139,-1.3821883766
246,T,-1.4237,403,5,-7.24887488225
246,V,-0.3869,215,106,-1.93643934363
246,W,-0.9252,19,1,-5.16409446205
246,Y,-0.2320,71,79,-0.762133319936
247,*,-0.4617,140,48,-2.46048746483
247,A,-0.7191,80,9,-4.06817004206
247,C,-1.0430,28,1,-5.72352187067
247,D,-0.2418,37,39,-0.840218095371
247,E,-0.2323,18,20,-0.764163855161
247,F,-0.7509,19,None,-4.24792751344
247,G,-0.8012,23,None,-4.52356195606
247,H,-0.2411,103,109,-0.834483151014
247,I,-0.9754,45,2,-5.40802004494
247,K,-0.3535,48,28,-1.69377452727
247,L,-0.3370,115,73,-1.57183244066
247,M,-0.4776,22,7,-2.56824364519
247,N,-0.4174,33,14,-2.1532061459
247,P,-0.7731,285,26,-4.37054533952
247,Q,0.000,None,None,None
247,R,-0.3347,137,88,-1.55476741292
247,S,-0.8015,122,10,-4.52497619128
247,T,-0.3086,87,64,-1.35911044445
247,V,-0.6768,30,4,-3.82305754421
247,W,-0.5697,9,None,-3.16992500144
247,Y,-0.6713,22,3,-3.79063606652
248,*,-0.3840,96,48,-1.9161669486
248,A,0.0811,225,1739,2.03409407748
248,C,-0.2694,43,39,-1.05702948445
248,D,-0.0198,105,415,1.06655505996
248,E,0.000,None,None,None
248,F,-0.3324,20,13,-1.53765532535
248,G,0.0548,111,717,1.77524649375
248,H,-0.1754,77,118,-0.300310439937
248,I,-0.4164,61,26,-2.14646456803
248,K,-0.1106,53,119,0.250730360137
248,L,-0.3368,159,101,-1.57083842114
248,M,-0.2681,23,21,-1.04741148188
248,N,-0.2103,43,54,-0.58754420115
248,P,-0.3159,322,228,-1.41419381256
248,Q,-0.0582,93,288,0.714599241733
248,R,-0.3268,145,97,-1.49616319643
248,S,0.0558,151,982,1.78500752639
248,T,-0.0640,163,487,0.662882859246
248,V,-0.1786,109,164,-0.326799268767
248,W,NS,2,2,-0.916166948604
248,Y,-0.3436,44,27,-1.62071106508
249,*,-1.4308,82,1,-7.27371895323
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QuickNormalize
Justin R. Klesmith
Contact: klesmit3@msu.edu, justinklesmith@gmail.com, justinklesmith@evodyn.com
Copyright 2015, Justin R. Klesmith
Version: 1.8, Build: 20150520
License: BSD-3
Credits: Justin R. Klesmith, Caitlin A. Kowalsky, Timothy A. Whitehead
Normalization run parameters:
16:21:55
05/22/2015
Start residue: 320
Normalization type: growth
Doublings: 12.8
Tile Length: 80
Enrich output directory: ./910/data/output-all/
Unselected counts to be significant: 5
Wild-type sequence file: ./WTSeq
Wild-type log2 (Ewt): 1.31305710501
Normalizing the data
Location,Mutation,Normalized_ER,Unselected_Reads,Selected_Reads,RawLog2
320,*,-1.1112,77,None,-6.26678654069
320,A,-0.2807,377,293,-1.18202763735
320,C,-1.0988,42,1,-6.21068120128
320,D,0.000,None,None,None
320,E,-0.1582,29,46,-0.15278281758
320,F,-1.0766,69,None,-6.10852445678
320,G,-0.4288,175,62,-2.31537857995
320,H,-0.1841,98,133,-0.377791187122
320,I,-1.3581,157,None,-7.29462074889
320,K,-0.6332,13,None,-3.70043971814
320,L,-1.5072,259,2,-7.8351720662
320,M,-0.6150,12,None,-3.58496250072
320,N,-0.2438,140,134,-0.881557604998
320,P,-1.6198,503,3,-8.20781586759
320,Q,-1.1416,48,1,-6.40332627923
320,R,-1.4917,220,None,-7.78135971352
320,S,-0.3271,284,171,-1.55025838313
320,T,-1.5937,279,None,-8.12412131183
320,V,-0.3758,200,93,-1.92306115718
320,W,-0.5744,10,None,-3.32192809489
320,Y,-0.6330,140,19,-3.69971928201
321,*,-0.4896,221,58,-2.74828534277
321,A,-0.8557,563,31,-5.0011585802
321,C,-1.3102,138,None,-7.10852445678
321,D,-0.3945,192,81,-2.06347627635
321,E,0.000,None,None,None
321,F,-1.5153,132,1,-7.86275789786
321,G,-0.3801,231,105,-1.95586730226
321,H,-1.7139,205,1,-8.49784387801
321,I,-1.5903,157,1,-8.1129845274
321,K,-0.2295,76,79,-0.762510543773
321,L,-2.2752,536,1,-9.88445296896
321,M,-0.7699,23,None,-4.52356195606
321,N,-1.1935,99,None,-6.62935662008
321,P,-1.6330,863,5,-8.2496524328
321,Q,-0.5729,169,30,-3.31235261918
321,R,-2.2236,498,1,-9.77836571057
321,S,-2.3543,597,1,-10.0399508998
321,T,-1.5120,231,None,-7.85174904142
321,V,-0.4033,186,75,-2.12870389912
321,W,-0.6959,17,None,-4.08746284125
321,Y,-1.3180,141,None,-7.1395513524
322,*,-1.5937,279,None,-8.12412131183
322,A,-1.8854,859,3,-8.97991559893
322,C,-1.2614,205,3,-6.91288137729
322,D,-1.8049,246,1,-8.76087828384
322,E,-1.4172,183,None,-7.51569983828
322,F,-0.6308,270,37,-3.68572600993
322,G,-1.9110,529,None,-9.04712391211
322,H,-1.9757,593,None,-9.21188829455
322,I,0.000,None,None,None
322,K,-1.6502,316,None,-8.30378074818
322,L,-0.2407,548,534,-0.855699929781
322,M,-0.9520,103,4,-5.50486430569
322,N,-0.7827,343,25,-4.59657235491
322,P,-1.9440,1909,6,-9.13200266519
322,Q,-1.7146,362,None,-8.49984588708
322,R,-1.5472,996,7,-7.97101078852
322,S,-1.9329,936,3,-9.10376599737
322,T,-1.0254,1158,35,-5.86650029955
322,V,-0.2629,184,158,-1.03814498639
322,W,-1.0338,60,None,-5.90689059561
322,Y,-1.6590,322,None,-8.33091687811
323,*,-1.3953,173,None,-7.43462822764
323,A,-2.5306,744,1,-10.3575225896
323,C,-0.6612,308,37,-3.87569695358
323,D,-1.5593,258,None,-8.01122725542
323,E,-1.5672,149,1,-8.03753229897
323,F,0.000,None,None,None
323,G,-1.7826,471,2,-8.69794702812
323,H,-2.1525,448,1,-9.62571870056
323,I,-0.6494,182,23,-3.80259646265
323,K,-1.3066,155,2,-7.09448818378
323,L,-0.6208,810,116,-3.62216088115
323,M,-1.1112,77,None,-6.26678654069
323,N,-1.8998,294,1,-9.01803612334
323,P,-2.1029,2074,5,-9.51463586244
323,Q,-1.9165,303,1,-9.06153776198
323,R,-1.9729,1004,3,-9.20494483174
323,S,-0.7300,966,87,-4.29129966149
323,T,-1.3000,761,10,-7.06818832712
323,V,-0.7393,173,15,-4.34610141053
323,W,-1.0338,60,None,-5.90689059561
323,Y,-0.1270,115,221,0.124048729941
324,*,-1.4527,200,None,-7.64385618977
324,A,-1.8815,853,3,-8.96980320911
324,C,-1.2599,120,None,-6.90689059561
324,D,-1.3817,167,None,-7.38370429247
324,E,-1.7186,207,1,-8.511850736
324,F,-0.6706,26,3,-3.93384099593
324,G,-1.8625,484,None,-8.91886323727
324,H,-1.7460,386,None,-8.59245703727
324,I,-0.0835,146,369,0.519288668672
324,K,-0.6758,186,21,-3.96520516684
324,L,0.0868,390,3135,2.18855563543
324,M,0.000,None,None,None
324,N,-1.7530,222,1,-8.61277964485
324,P,-2.3899,2502,4,-10.1072298527
324,Q,-1.6264,300,None,-8.2288186905
324,R,-1.3443,1030,12,-7.24182989986
324,S,-2.1171,748,None,-9.54689445989
324,T,-0.7495,733,61,-4.40529582908
324,V,-0.2092,171,200,-0.59236010362
324,W,-0.9739,49,None,-5.61470984412
324,Y,-1.3180,141,None,-7.1395513524
325,*,-0.6809,172,19,-3.99670101977
325,A,-2.0909,407,1,-9.48724876277
325,C,0.000,None,None,None
325,D,-1.2629,121,None,-6.91886323727
325,E,-1.1726,93,None,-6.53915881111
325,F,-0.1909,155,202,-0.436276701029
325,G,-1.0610,372,10,-6.03559449473
325,H,-1.8332,458,None,-8.8392037881
325,I,-1.5229,237,None,-7.8887432489
325,K,-0.9021,38,None,-5.24792751344
325,L,-2.4003,1118,None,-10.1267044728
325,M,-1.0534,64,None,-6.0
325,N,-1.3360,148,None,-7.20945336563
325,P,-1.9398,3474,11,-9.12131419858
325,Q,-1.3636,271,3,-7.31555031914
325,R,-0.8119,1322,86,-4.76060548531
325,S,-0.4256,489,176,-2.29262281482
325,T,-2.2685,531,1,-9.87093182931
325,V,-1.5905,277,None,-8.11374216605
325,W,-0.7350,68,6,-4.32086411904
325,Y,-0.4444,116,38,-2.42841726019
326,*,-0.9761,28,1,-5.62571870056
326,A,-0.9455,302,12,-5.47180601711
326,C,-0.2948,103,74,-1.29541094006
326,D,-0.4797,138,38,-2.67896072184
326,E,-0.8470,31,None,-4.95419631039
326,F,-0.8796,35,None,-5.12928301694
326,G,0.000,None,None,None
326,H,-1.1654,91,None,-6.5077946402
326,I,-0.8873,36,None,-5.16992500144
326,K,-0.9232,41,None,-5.35755200462
326,L,-1.5599,293,2,-8.01312063293
326,M,-0.6332,13,None,-3.70043971814
326,N,-1.0766,69,None,-6.10852445678
326,P,-1.7170,619,3,-8.507196877
326,Q,-0.8873,36,None,-5.16992500144
326,R,-0.8223,384,24,-4.81836377851
326,S,-0.5110,456,108,-2.89636629052
326,T,-1.3770,165,None,-7.36632221425
326,V,-0.4822,125,34,-2.69668522192
326,W,-0.6959,17,None,-4.08746284125
326,Y,-0.9912,52,None,-5.70043971814
327,*,-0.8796,35,None,-5.12928301694
327,A,-0.7853,194,14,-4.61092169864
327,C,-0.1267,40,77,0.126494667306
327,D,-0.3563,66,34,-1.77529505662
327,E,-0.6332,13,None,-3.70043971814
327,F,-0.8055,30,2,-4.72525437412
327,G,0.000,None,None,None
327,H,-0.8384,30,None,-4.90689059561
327,I,-1.0437,62,None,-5.95419631039
327,K,-0.6815,16,None,-4.0
327,L,-1.2480,116,None,-6.85798099513
327,M,NS,3,None,None
327,N,-0.6815,16,None,-4.0
327,P,-1.7348,214,1,-8.55983076491
327,Q,-0.6663,15,None,-3.90689059561
327,R,-0.9270,165,7,-5.3773310707
327,S,-0.5308,204,44,-3.03135750184
327,T,-0.6159,123,18,-3.59095328241
327,V,-0.1888,50,66,-0.417825848929
327,W,NS,4,None,None
327,Y,-0.8948,37,None,-5.20945336563
328,*,-0.8108,27,None,-4.75488750216
328,A,-0.9774,225,8,-5.63214496973
328,C,-0.3910,142,61,-2.03737356045
328,D,-0.4786,112,31,-2.67152239018
328,E,-0.4981,7,None,-2.80735492206
328,F,-0.7491,24,2,-4.40332627923
328,G,0.000,None,None,None
328,H,-0.9093,39,None,-5.28540221886
328,I,-0.6815,16,None,-4.0
328,K,-0.4981,7,None,-2.80735492206
328,L,-1.3284,145,None,-7.17990909001
328,M,NS,4,None,None
328,N,-0.9798,50,None,-5.64385618977
328,P,-1.5193,235,None,-7.87651694657
328,Q,-0.7699,23,None,-4.52356195606
328,R,-1.0323,237,7,-5.89975210535
328,S,-0.4518,209,66,-2.48132879123
328,T,-1.0338,60,None,-5.90689059561
328,V,-0.4495,216,69,-2.4647268239
328,W,-0.7473,21,None,-4.39231742278
328,Y,-0.6959,17,None,-4.08746284125
329,*,-0.9163,40,None,-5.32192809489
329,A,0.000,None,None,None
329,C,-0.3511,51,27,-1.73590161832
329,D,-0.4931,62,16,-2.7725600889
329,E,-0.6502,14,None,-3.80735492206
329,F,-0.9557,46,None,-5.52356195606
329,G,-1.1836,109,2,-6.58654810329
329,H,-1.3700,162,None,-7.33985000288
329,I,-1.0236,58,None,-5.85798099513
329,K,-0.5262,8,None,-3.0
329,L,-1.2527,200,3,-6.87725746756
329,M,NS,2,None,None
329,N,-0.7473,21,None,-4.39231742278
329,P,-0.9794,793,28,-5.64218591215
329,Q,-1.0899,72,None,-6.16992500144
329,R,-1.2597,68,1,-6.90582661976
329,S,-0.1262,115,222,0.130562036899
329,T,-0.4094,261,102,-2.17384443311
329,V,-0.5491,101,20,-3.15464716638
329,W,-0.7473,21,None,-4.39231742278
329,Y,-0.9855,51,None,-5.67242534197
330,*,-0.2850,58,44,-1.216913155
330,A,-0.3028,16,11,-1.35893215987
330,C,0.0246,27,140,1.55603173628
330,D,-0.1434,19,33,-0.0218971725934
330,E,-0.8223,16,1,-4.81836377851
330,F,-0.0418,32,106,0.909556676054
330,G,-0.8718,34,None,-5.08746284125
330,H,-0.1170,132,270,0.214057699187
330,I,-0.5954,11,None,-3.45943161864
330,K,NS,4,4,-0.818363778502
330,L,-0.0727,127,344,0.619216289422
330,M,-0.3619,6,3,-1.81836377851
330,N,-0.0993,21,48,0.374281299433
330,P,-1.3601,358,4,-7.30217955577
330,Q,-0.1674,10,15,-0.233401277783
330,R,0.0248,42,218,1.55750312349
330,S,-0.2049,80,96,-0.555329372678
330,T,-1.2329,63,1,-6.79564370201
330,V,-0.1531,11,18,-0.107870395701
330,W,NS,2,4,0.181636221493
330,Y,0.000,None,None,None
331,*,-0.6150,12,None,-3.58496250072
331,A,-1.3975,174,None,-7.44294349585
331,C,-0.9761,28,1,-5.62571870056
331,D,-0.2772,53,42,-1.15396681029
331,E,-0.4297,5,None,-2.32192809489
331,F,-0.8555,32,None,-5.0
331,G,-1.0766,69,None,-6.10852445678
331,H,-0.0625,331,958,0.714830660418
331,I,-0.0916,25,60,0.444670627326
331,K,-0.2362,24,24,-0.81836377851
331,L,-0.9366,43,None,-5.4262647547
331,M,NS,1,None,None
331,N,0.000,None,None,None
331,P,-1.9120,530,None,-9.04984854945
331,Q,-0.8295,29,None,-4.85798099513
331,R,-1.2658,122,None,-6.93073733756
331,S,-0.4885,163,43,-2.74082717804
331,T,-0.7310,301,27,-4.2970959531
331,V,-0.8948,37,None,-5.20945336563
331,W,NS,2,None,None
331,Y,-0.5952,81,13,-3.45777406325
332,*,-0.8470,31,None,-4.95419631039
332,A,-0.4284,62,22,-2.31312847026
332,C,-0.9436,25,1,-5.46221996828
332,D,-0.4981,7,None,-2.80735492206
332,E,-0.3421,9,5,-1.66636068506
332,F,NS,2,None,None
332,G,-0.8678,19,1,-5.06629129195
332,H,-0.8950,21,1,-5.21068120129
332,I,-0.5868,12,2,-3.40332627923
332,K,-0.5868,12,2,-3.40332627923
332,L,-0.2970,155,110,-1.31312847026
332,M,-0.7277,11,1,-4.27779539714
332,N,-0.6150,12,None,-3.58496250072
332,P,0.000,None,None,None
332,Q,-0.2477,47,44,-0.913521011544
332,R,-0.5566,115,22,-3.20442221081
332,S,-0.2855,78,59,-1.22112294801
332,T,-0.0482,73,232,0.849792657741
332,V,0.0395,20,115,1.70519817755
332,W,-0.6332,13,None,-3.70043971814
332,Y,-0.6332,13,None,-3.70043971814
333,*,-1.1762,94,None,-6.55458885168
333,A,-1.2518,133,2,-6.87364621401
333,C,-0.3563,33,17,-1.77529505662
333,D,-0.2327,49,50,-0.789217432847
333,E,-0.7352,20,None,-4.32192809489
333,F,-0.8948,37,None,-5.20945336563
333,G,-0.9366,43,None,-5.4262647547
333,H,-0.5382,72,15,-3.08139818434
333,I,-0.1295,37,70,0.101465872806
333,K,-0.3336,43,25,-1.60077234344
333,L,-1.4293,107,1,-7.55983076491
333,M,-0.6663,15,None,-3.90689059561
333,N,0.000,None,None,None
333,P,-1.8833,503,None,-8.97441458981
333,Q,-0.9299,42,None,-5.39231742278
333,R,-1.4466,197,None,-7.62205181946
333,S,-0.5348,222,47,-3.05819079318
333,T,-0.5406,165,34,-3.0972231515
333,V,-1.0023,54,None,-5.75488750216
333,W,-0.5744,10,None,-3.32192809489
333,Y,-0.2123,74,85,-0.618426207991
334,*,-0.7227,19,None,-4.24792751344
334,A,-1.2419,114,None,-6.83289001416
334,C,-0.9619,47,None,-5.55458885168
334,D,-0.5514,9,None,-3.16992500144
334,E,NS,4,None,None
334,F,-0.2042,44,53,-0.54987494258
334,G,-1.1353,83,None,-6.37503943135
334,H,-1.0437,62,None,-5.95419631039
334,I,0.000,None,None,None
334,K,-0.9431,44,None,-5.45943161864
334,L,-0.3984,133,55,-2.09228650049
334,M,-0.4204,46,17,-2.25446289332
334,N,-0.2886,39,29,-1.24578500224
334,P,-1.5743,267,None,-8.06069593169
334,Q,-1.0023,54,None,-5.75488750216
334,R,-1.3154,140,None,-7.12928301694
334,S,-0.5110,190,45,-2.89636629051
334,T,-0.2732,116,94,-1.12175592196
334,V,-0.3650,63,31,-1.84144739162
334,W,-0.5262,8,None,-3.0
334,Y,-1.1468,86,None,-6.4262647547
335,*,-0.9495,45,None,-5.49185309633
335,A,-0.0529,147,453,0.805331116701
335,C,-0.4357,38,13,-2.36585157381
335,D,-1.0629,66,None,-6.04439411936
335,E,-0.2391,60,59,-0.842611324758
335,F,-0.7588,22,None,-4.45943161864
335,G,-0.4679,120,35,-2.59597135717
335,H,-0.8011,26,None,-4.70043971814
335,I,-0.0210,41,156,1.10948643573
335,K,-0.3619,16,8,-1.81836377851
335,L,-0.1206,164,328,0.18163622149
335,M,-0.1166,40,82,0.217260131225
335,N,-0.8796,35,None,-5.12928301694
335,P,-1.5157,233,None,-7.86418614465
335,Q,-0.3344,38,22,-1.60685967332
335,R,-0.2581,102,90,-0.998936024149
335,S,-0.3444,133,73,-1.68382165513
335,T,-0.0389,53,179,0.937531544193
335,V,0.000,None,None,None
335,W,-0.0873,15,37,0.484198991511
335,Y,-0.6795,27,3,-3.98828877995
336,*,-0.3151,98,63,-1.45579369912
336,A,-0.2231,191,206,-0.709292079361
336,C,-1.0721,68,None,-6.08746284125
336,D,-0.0200,76,291,1.11858405096
336,E,0.000,None,None,None
336,F,-0.7352,20,None,-4.32192809489
336,G,-0.1293,38,72,0.103633709492
336,H,-0.3605,129,65,-1.8072232209
336,I,-0.8108,27,None,-4.75488750216
336,K,-0.1980,44,55,-0.49643568362
336,L,-1.5139,232,None,-7.85798099513
336,M,NS,4,None,None
336,N,-0.1388,28,50,0.0181374892089
336,P,-0.7761,882,66,-4.55860450471
336,Q,-0.1596,82,129,-0.164688527704
336,R,-0.8364,152,9,-4.89636629051
336,S,-0.1696,77,114,-0.252260305038
336,T,-0.6248,135,19,-3.64725186211
336,V,-0.4221,161,59,-2.26663760726
336,W,NS,2,None,None
336,Y,-1.0582,65,None,-6.02236781303
337,*,-0.7096,18,None,-4.16992500144
337,A,-1.4363,192,None,-7.58496250072
337,C,-0.2395,54,53,-0.845330826106
337,D,-0.8718,34,None,-5.08746284125
337,E,-0.8873,36,None,-5.16992500144
337,F,0.000,None,None,None
337,G,-0.9202,69,3,-5.34192573456
337,H,-0.2231,76,82,-0.708739287335
337,I,-0.2174,26,29,-0.660822501522
337,K,-0.5744,10,None,-3.32192809489
337,L,-0.4922,301,78,-2.76658123641
337,M,-0.5954,11,None,-3.45943161864
337,N,-0.8718,34,None,-5.08746284125
337,P,-3.4234,1625,1,-11.4845877813
337,Q,-1.0855,71,None,-6.1497471195
337,R,-1.5034,385,3,-7.82211591337
337,S,-0.5929,333,54,-3.44285464341
337,T,-1.4506,199,None,-7.63662462054
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Q,-1.1416,-0.5729,-1.7146,-1.9165,-1.6264,-1.3636,-0.8873,-0.6663,-0.7699,-1.0899,-0.1674,-0.8295,-0.2477,-0.9299,-1.0023,-0.3344,-0.1596,-1.0855,-1.0582,0.000,0.000,-0.0915,-1.0023,-0.5118,-0.0135,-0.6815,-0.0087,-1.6314,-0.5954,-0.7076,-0.8503,-1.2450,-0.2034,-1.2384,-0.8948,-0.6502,-0.4997,-1.1615,-0.1954,-1.2857,-0.0323,-0.0126,-1.0582,-1.1615,-0.6150,-0.5262,-0.9680,-1.3557,0.000,-1.1867,-0.9619,-0.4409,-0.8384,-0.5727,-1.3019,-1.5494,-1.3334,-1.2046,-0.8678,-0.3808,-0.5514,-0.3992,-0.0805,-0.4662,NS,-0.3351,-0.4297,-0.2067,0.000,-0.2278,-0.0107,-0.5744,-0.4803,-0.6959,-0.7314,-0.6332,-0.8108,-0.4049,-0.5262,-0.0204,
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S,-0.3271,-2.3543,-1.9329,-0.7300,-2.1171,-0.4256,-0.5110,-0.5308,-0.4518,-0.1262,-0.2049,-0.4885,-0.2855,-0.5348,-0.5110,-0.3444,-0.1696,-0.5929,-1.4664,-0.3361,-0.0566,0.000,-1.2104,-0.3943,-0.1073,-0.0349,-0.0990,-0.8946,-1.0899,-0.7604,-2.0436,-1.8517,-0.2001,-0.5066,-0.7836,-1.5405,-0.4940,-0.1717,-0.1040,-0.1231,-1.4858,-0.8470,-0.2941,-0.5521,-0.3146,-0.3249,-0.3070,-0.9086,-1.0988,-0.4981,-1.5433,-1.1543,-0.4192,-1.6801,-1.1753,-0.5971,-0.9704,0.000,-1.2180,-0.4005,-1.1836,-0.4721,-0.0376,-0.5354,-1.2544,-1.1679,-0.1489,-0.4049,-0.1358,-0.0009,0.1484,-0.6332,-1.5341,-0.5995,-1.5584,-1.0943,0.000,-0.4570,-0.4736,-0.0752,
T,-1.5937,-1.5120,-1.0254,-1.3000,-0.7495,-2.2685,-1.3770,-0.6159,-1.0338,-0.4094,-1.2329,-0.7310,-0.0482,-0.5406,-0.2732,-0.0389,-0.6248,-1.4506,-0.6148,-1.2371,-0.1539,-0.3175,-1.5731,-0.4915,-0.1397,0.000,-0.1554,-0.6716,-0.2362,-0.3388,-1.5542,-1.3896,-0.2869,-0.0892,-1.1543,-1.2940,-0.3934,-0.0797,-0.0647,-0.5477,-0.7909,-0.7473,-0.5951,-0.3602,0.000,-0.7807,-0.4080,-1.8438,-1.3407,-1.1362,-0.4230,-1.0855,-0.9656,-1.6662,-1.3318,-1.9091,-1.6207,-0.8101,-0.3798,-0.4409,-1.0388,-0.6833,0.000,-0.8382,-0.8678,-0.8203,0.000,-0.4297,-0.1501,-1.0060,0.0859,-0.4981,-1.2570,-0.9091,-0.6467,-1.0154,-0.3925,-0.3710,-1.2480,-0.0961,
V,-0.3758,-0.4033,-0.2629,-0.7393,-0.2092,-1.5905,-0.4822,-0.1888,-0.4495,-0.5491,-0.1531,-0.8948,0.0395,-1.0023,-0.3650,0.000,-0.4221,-0.4424,0.0135,-1.1274,-0.2715,-1.0635,-0.8295,-0.7911,-1.0467,-0.5322,-0.3290,-0.1400,-0.1158,-0.2606,-0.5977,-0.4284,-0.1053,-0.2219,-0.6786,0.000,-0.7699,-0.1135,-0.1430,-0.4869,-0.7879,-0.0602,-0.1349,-0.1240,-0.5262,-0.4175,-0.4159,-1.2994,-1.0721,-0.3485,-0.1595,-0.2037,-0.8796,-0.2896,0.000,-0.7277,-1.3102,-1.1506,-0.0423,-0.7699,0.000,-0.5514,-0.0981,-0.8433,0.000,-0.2253,-0.4981,-0.7352,0.0163,-0.8055,-0.2309,0.000,-0.6461,-0.3539,-0.8582,0.000,-0.7911,-0.8011,-0.3542,-0.0135,
W,-0.5744,-0.6959,-1.0338,-1.0338,-0.9739,-0.7350,-0.6959,NS,-0.7473,-0.7473,NS,NS,-0.6332,-0.5744,-0.5262,-0.0873,NS,-0.5868,-0.5262,-0.8203,NS,-0.3389,-0.6959,-0.5514,-0.6520,-0.4663,-0.6502,-0.5744,NS,-0.8718,-0.8470,-0.6332,-1.2161,-0.7096,-0.6815,-0.7588,NS,-0.7352,-0.5245,-0.7473,-0.4297,NS,-0.6502,-0.5954,-0.4663,NS,NS,0.000,-0.8817,-0.7699,-0.8108,-0.4297,-0.2836,-0.9963,-1.0336,-0.7699,-0.7227,-0.7096,-0.7691,-0.5954,-0.7096,-0.4981,-0.4297,-0.2568,NS,-0.4297,NS,NS,-0.4663,NS,-0.1145,NS,-0.7699,-0.6150,-0.7588,NS,-0.4663,NS,-0.8108,NS,
Y,-0.6330,-1.3180,-1.6590,-0.1270,-1.3180,-0.4444,-0.9912,-0.8948,-0.6959,-0.9855,0.000,-0.5952,-0.6332,-0.2123,-1.1468,-0.6795,-1.0582,-0.0440,-1.1774,-1.2104,-0.2541,-0.4377,0.000,-0.6663,-0.1545,-0.9431,-0.6882,-1.3930,-0.0905,-0.4364,-0.8203,-0.3646,-0.3946,-1.1416,-1.1112,-0.8011,-0.4663,-0.9232,-0.4409,-1.0437,-0.8382,NS,-0.9163,-1.0336,-0.5744,-0.1305,-0.9232,-0.9232,-1.0023,-1.3747,-0.9830,-0.8796,-0.1713,-1.3360,-1.0445,-1.5121,-1.2003,-0.8678,-0.8948,-0.6214,-1.0593,-0.4663,-0.2362,-0.4817,-0.7588,-0.6502,NS,-0.5262,-0.8382,-0.1764,0.000,NS,-0.3704,-0.9163,-0.8637,-0.7096,-0.7473,-0.4297,-0.6371,NS,

QuickNormalize
Justin R. Klesmith
Contact: klesmit3@msu.edu, justinklesmith@gmail.com, justinklesmith@evodyn.com
Copyright 2015, Justin R. Klesmith
Version: 1.8, Build: 20150520
License: BSD-3
Credits: Justin R. Klesmith, Caitlin A. Kowalsky, Timothy A. Whitehead
Normalization run parameters:
16:21:55
05/22/2015
Start residue: 400
Normalization type: growth
Doublings: 13.2
Tile Length: 39
Enrich output directory: ./11/data/output-all/
Unselected counts to be significant: 5
Wild-type sequence file: ./WTSeq
Wild-type log2 (Ewt): 0.696641380963
Normalizing the data
Location,Mutation,Normalized_ER,Unselected_Reads,Selected_Reads,RawLog2
400,*,-0.9532,65,None,-6.02236781303
400,A,-1.2700,162,3,-7.43780312534
400,C,-0.8197,50,4,-5.32677181295
400,D,-0.3068,45,37,-1.96531535388
400,E,-0.5950,16,None,-4.0
400,F,-1.1396,37,1,-6.89236898881
400,G,-0.9253,59,None,-5.88264304936
400,H,-0.9061,103,6,-5.78445364964
400,I,-0.4054,59,29,-2.70757767741
400,K,-0.3095,58,47,-1.98630776662
400,L,-1.6030,123,1,-8.62543012851
400,M,-0.4924,10,None,-3.32192809489
400,N,0.000,None,None,None
400,P,-1.2289,433,9,-7.27121383646
400,Q,-1.1055,107,None,-6.7414669864
400,R,-1.9252,232,1,-9.5408966183
400,S,-0.6353,297,50,-4.2533785542
400,T,-0.8352,212,16,-5.41083607774
400,V,-1.3328,64,1,-7.68291562317
400,W,-0.8381,43,None,-5.4262647547
400,Y,-0.2755,40,39,-1.7194414992
401,*,-0.1692,53,95,-0.840980469403
401,A,-1.2672,172,None,-7.4262647547
401,C,-0.4200,81,37,-2.81331226043
401,D,-0.6654,81,12,-4.43780312534
401,E,-0.6344,19,None,-4.24792751344
401,F,-0.1460,34,70,-0.641095447481
401,G,-0.9620,67,None,-6.06608919046
401,H,-0.4056,112,55,-2.70891083171
401,I,-1.2633,53,1,-7.41083607774
401,K,-0.6464,20,None,-4.32192809489
401,L,-1.3169,197,None,-7.62205181946
401,M,-0.6344,19,None,-4.24792751344
401,N,-0.7260,52,6,-4.7983928406
401,P,-1.6564,414,3,-8.79144007996
401,Q,-1.1306,36,1,-6.85284062462
401,R,-1.3208,248,4,-7.63711193357
401,S,-0.9326,245,13,-5.91911384403
401,T,-0.6899,172,23,-4.58561842182
401,V,-1.3833,73,1,-7.87274018206
401,W,NS,4,None,None
401,Y,0.000,None,None,None
402,*,-0.6464,20,None,-4.32192809489
402,A,-1.1655,120,3,-7.00484371806
402,C,-0.2967,76,66,-1.88644901726
402,D,-0.6088,17,None,-4.08746284125
402,E,-0.4462,8,None,-3.0
402,F,-0.8865,16,1,-5.68291562318
402,G,-0.8301,52,4,-5.38335534132
402,H,-0.4502,155,61,-3.02830269089
402,I,-0.8687,15,1,-5.58980621879
402,K,-0.5314,12,None,-3.58496250072
402,L,-0.5532,157,38,-3.72960885862
402,M,NS,2,None,None
402,N,-0.8442,44,None,-5.45943161864
402,P,-0.7978,184,16,-5.20647757923
402,Q,-1.1738,41,1,-7.04046762779
402,R,0.000,None,None,None
402,S,-0.4446,89,36,-2.9887240527
402,T,-1.2832,56,1,-7.49027054523
402,V,-0.7984,37,None,-5.20945336563
402,W,-0.4462,8,None,-3.0
402,Y,-0.5950,16,None,-4.0
403,*,-0.6689,22,None,-4.45943161864
403,A,0.0015,101,531,0.711440944878
403,C,-0.2646,28,29,-1.6322895501
403,D,-0.2093,12,17,-1.18041528264
403,E,-0.1884,5,8,-1.00484371805
403,F,-0.1972,50,76,-1.07884429951
403,G,-0.2262,14,18,-1.32034554379
403,H,-0.1233,33,78,-0.441907523673
403,I,-0.1394,14,30,-0.583379949625
403,K,-0.3203,17,13,-2.06993874628
403,L,-0.2182,78,105,-1.25407232437
403,M,NS,1,4,0.317084376825
403,N,-0.1343,19,42,-0.538525713841
403,P,-0.0250,293,1292,0.457717877101
403,Q,-0.0400,23,92,0.317084376829
403,R,-0.0668,41,138,0.0680568289872
403,S,0.000,None,None,None
403,T,0.0236,119,726,0.926092351514
403,V,-0.1565,30,58,-0.731825223652
403,W,-0.5461,8,2,-3.68291562318
403,Y,-0.0752,21,67,-0.00914385549426
404,*,-1.3779,72,1,-7.85284062462
404,A,-0.6092,488,92,-4.09009100467
404,C,-1.1151,103,3,-6.78445364964
404,D,-1.1055,107,None,-6.7414669864
404,E,-0.4459,112,45,-2.9984174489
404,F,-0.9487,64,None,-6.0
404,G,-0.6936,372,49,-4.60736459016
404,H,-1.5571,111,1,-8.47733148953
404,I,-0.1754,22,38,-0.894419728368
404,K,-0.7124,41,5,-4.7185395329
404,L,-0.4223,155,70,-2.8297570115
404,M,-0.3000,68,58,-1.9123974693
404,N,-0.9707,43,2,-6.10918037788
404,P,-1.7591,681,4,-9.09442661119
404,Q,-1.2667,107,2,-7.42438260958
404,R,-1.6201,383,3,-8.67915370438
404,S,-0.6923,287,38,-4.5998950364
404,T,-0.7379,100,11,-4.86734019431
404,V,0.000,None,None,None
404,W,-0.9399,58,3,-5.95593411758
404,Y,-0.8790,50,None,-5.64385618977
405,*,-1.5266,207,2,-8.37640258068
405,A,-0.9830,269,12,-6.16941548501
405,C,-0.9831,72,None,-6.16992500144
405,D,-1.0884,158,5,-6.66476827646
405,E,-1.6733,143,1,-8.84278695995
405,F,-1.3444,66,1,-7.72730974254
405,G,-1.3779,144,2,-7.85284062462
405,H,-2.0451,284,1,-9.83266274268
405,I,-0.5031,249,76,-3.3949900418
405,K,-0.6441,185,30,-4.30740648808
405,L,-0.1765,430,738,-0.903631466706
405,M,0.000,None,None,None
405,N,-1.5956,242,2,-8.60177886045
405,P,-1.3709,990,14,-7.82684541609
405,Q,-1.6615,279,2,-8.807036935
405,R,-1.1071,536,16,-6.74900481363
405,S,-1.5139,402,4,-8.33396731435
405,T,-0.7155,623,75,-4.7371852857
405,V,-0.4729,156,55,-3.18695812851
405,W,-1.1213,35,1,-6.81219864012
405,Y,-1.3697,141,2,-7.82246697558
406,*,-0.5289,218,59,-3.56845689859
406,A,-0.2574,191,206,-1.57384392403
406,C,-1.6067,124,1,-8.63711193356
406,D,-1.2717,217,4,-7.44446685562
406,E,-0.4418,139,57,-2.96896668173
406,F,-1.3991,76,1,-7.93084313662
406,G,-0.9756,153,7,-6.13294854381
406,H,-0.7332,223,25,-4.83995933331
406,I,-0.5113,160,47,-3.45025486638
406,K,0.000,None,None,None
406,L,-1.1200,244,7,-6.80629803869
406,M,-0.6377,18,3,-4.2678781239
406,N,-0.6298,203,35,-4.21896852341
406,P,-1.6591,694,5,-8.79977938087
406,Q,-0.2210,123,163,-1.27670197428
406,R,0.1039,336,3590,1.73453508276
406,S,-0.1955,239,367,-1.06414617832
406,T,-0.4650,325,119,-3.13239376778
406,V,-1.4743,183,2,-8.19861546146
406,W,-0.6047,26,5,-4.06142724643
406,Y,-0.9678,149,7,-6.09472922158
407,*,-0.7619,270,27,-5.00484371806
407,A,-0.2164,383,521,-1.23897664282
407,C,-1.3208,124,2,-7.63711193356
407,D,-1.3425,197,3,-7.72000494191
407,E,-0.4462,152,61,-3.00010579905
407,F,-1.0709,96,None,-6.58496250072
407,G,-1.3283,253,4,-7.66590919786
407,H,-1.0276,208,8,-6.38335534131
407,I,-0.7581,266,27,-4.98331055651
407,K,0.000,None,None,None
407,L,-1.1238,529,15,-6.82314893968
407,M,-0.7682,41,4,-5.04046762779
407,N,-0.6385,277,46,-4.27309583317
407,P,-1.2476,1268,25,-7.34739846354
407,Q,-0.4385,168,70,-2.94595002901
407,R,0.0060,300,1625,0.754489689135
407,S,-0.7594,505,51,-4.99062985884
407,T,-0.9253,790,43,-5.88235971154
407,V,-1.1889,300,7,-7.10437939161
407,W,-0.9004,54,None,-5.75488750216
407,Y,-1.8241,193,1,-9.27537266045
408,*,-0.4704,9,None,-3.16992500144
408,A,-0.9638,126,6,-6.07523304595
408,C,-0.4238,29,13,-2.84045690016
408,D,-0.4489,101,40,-3.01919901104
408,E,NS,4,1,-3.68291562318
408,F,-1.1484,38,1,-6.93084313662
408,G,0.000,None,None,None
408,H,-0.9748,70,None,-6.12928301694
408,I,-0.7863,11,1,-5.14234724181
408,K,-0.4192,7,None,-2.80735492206
408,L,-0.9730,65,3,-6.12032093548
408,M,NS,1,None,None
408,N,-0.6796,23,None,-4.52356195606
408,P,-1.6546,550,4,-8.78620343158
408,Q,-0.5127,11,None,-3.45943161864
408,R,-1.1619,277,7,-6.98930286717
408,S,-0.4559,81,31,-3.06856931567
408,T,-0.7840,35,None,-5.12928301694
408,V,-0.3062,80,66,-1.9604495987
408,W,-0.7063,8,1,-4.68291562318
408,Y,-0.5651,14,None,-3.80735492206
409,*,-1.7990,184,1,-9.20647757923
409,A,-0.8929,262,16,-5.71633862471
409,C,-0.9496,100,5,-6.00484371806
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Normalized Heatmap
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