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run_ScarMapper_Custom.sh
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#!/bin/bash
#Parameter file to run ScarMapper
#File generated 2020-01-19
python3 /mnt/hgfs/OneDrive_UNC/Projects/Programs/ScarMapper/scarmapper.py --options_file /mnt/hgfs/Drive_D/ScarMapper_Data/run_ScarMapper_Custom.sh
exit
--IndelProcessing True
--FASTQ1 /mnt/hgfs/Drive_D/ScarMapper_Data/RJ_JCG1_S1_L001_R1_001.fastq.gz
--FASTQ2 /mnt/hgfs/Drive_D/ScarMapper_Data/RJ_JCG1_S1_L001_R2_001.fastq.gz
--RefSeq /mnt/hgfs/OneDrive/Bioinformatics/RefSeq/GRCm38/GRCm38.p6.fa.gz
--Master_Index_File /mnt/hgfs/OneDrive/Bioinformatics/Indices/Master_Lists/Ramsden_Rosa26a_Oligos.csv
--SampleManifest /mnt/hgfs/Drive_D/ScarMapper_Data/RamsdenSampleManifest.csv
--TargetFile /mnt/hgfs/OneDrive_UNC/Projects/ScarMapper_Runs/ScarMapper_Targets.txt
--PrimerPhasingFile /mnt/hgfs/OneDrive_UNC/Projects/ScarMapper_Runs/PrimerPhasing.txt
--WorkingFolder /mnt/hgfs/Drive_D/ScarMapper_Data/
--Verbose DEBUG
--Job_Name Testing
--Spawn 5
--Demultiplex False
--Species Mouse
--Cell_Line hTERT_RPE1
--Platform Ramsden
--N_Limit 0.1
--Minimum_Length 100 # Length after trimming
--OutputRawData False